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At least 91 records · Page 5Linked to original sources

Small mammal populations at hazardous waste disposal sites near Houston, Texas, USA

Small mammals were trapped, tagged and recaptured in 0?45 ha plots at six hazardous industrial waste disposal sites to determine if populations, body mass and age structures were different from paired control site plots. Low numbers of six species of small mammals were captured on industrial waste sites or control sites. Only populations of hispid cotton rats at industrial waste sites and control sites were large enough for comparisons. Overall population numbers, age structure, and body mass of adult male and female cotton rats were similar at industrial waste sites and control sites. Populations of small mammals (particularly hispid cotton rats) may not suffice as indicators of environments with hazardous industrial waste contamination.

Environmental Pollution↗

Detection limits and cost comparisons of human- and gull-associated conventional and quantitative PCR assays in artificial and environmental waters

Some molecular methods for tracking fecal pollution in environmental waters have both PCR and quantitative PCR (qPCR) assays available for use. To assist managers in deciding whether to implement newer qPCR techniques in routine monitoring programs, we compared detection limits (LODs) and costs of PCR and qPCR assays with identical targets that are relevant to beach water quality assessment. For human-associated assays targeting Bacteroidales HF183 genetic marker, qPCR LODs were 70 times lower and there was no effect of target matrix (artificial freshwater, environmental creek water, and environmental marine water) on PCR or qPCR LODs. The PCR startup and annual costs were the lowest, while the per reaction cost was 62% lower than the Taqman based qPCR and 180% higher than the SYBR based qPCR. For gull-associated assays, there was no significant difference between PCR and qPCR LODs, target matrix did not effect PCR or qPCR LODs, and PCR startup, annual, and per reaction costs were lower. Upgrading to qPCR involves greater startup and annual costs, but this increase may be justified in the case of the human-associated assays with lower detection limits and reduced cost per sample.

Journal of Environmental Management↗

Organochlorine contaminants in eggs of common terns from the Canadian Great Lakes, 1981

To determine if contaminant levels in Common Terns had changed over the last decade, we collected and analyzed eggs from four nesting colonies on the three lower Great Lakes during 1981. DDE and PCBs were detected in every egg from the four colonies. Dieldrin, mirex and trans -nonachlor were detected in more than 45% of the eggs. Seven other organochlorine contaminants (DDD, DDT, hexachlorobenzene, oxychlordane, cis -chlordane, cis -nonachlor and toxaphene) were detected in less than 25% of the eggs. Eggs from the Lake Ontario colony were generally the most heavily contaminated. Comparisons of DDE and PCB data with earlier studies of Common Terns indicated that contaminant levels in eggs from the four sampled colonies, or nearby sites, have decreased by up to 80–90% from 1969–1973 to 1981. Interspecies comparisons showed that Common Tern eggs have lower organochlorine residue levels than eggs of Caspian Terns or Herring Gulls. Dietary variation and migratory status are possible explanations for the differences in residue levels among species. Eggshell thickness, log-PCBs, and log-DDE were not significantly intercorrelated. Elevated contaminant levels in the early 1970s might be at least partly responsible for the decline of the Great Lakes Common Tern population over the past decade. Stabilization of population numbers during the early 1980s suggests that organochlorine pollution levels have been reduced to a point where they are no longer an important factor in the population dynamics of this species on the Great Lakes.

Fighting Island, Georgian Bay, Great Lakes, Lake E↗

Improving the ecological relevance of toxicity tests on scleractinian corals: Influence of season, life stage, and seawater temperature

Metal pollutants in marine systems are broadly acknowledged as deleterious: however, very little data exist for tropical scleractinian corals. We address this gap by investigating how life-history stage, season and thermal stress influence the toxicity of copper (Cu) and lead (Pb) in the coral Pocillopora damicornis . Our results show that under ambient temperature, adults and larvae appear to tolerate exposure to unusually high levels of copper (96 h-LC 50 ranging from 167 to 251 μg Cu L −1 ) and lead (from 477 to 742 μg Pb L −1 ). Our work also highlights that warmer conditions (seasonal and experimentally manipulated) reduce the tolerance of adults and larvae to Cu toxicity. Despite a similar trend observed for the response of larvae to Pb toxicity to experimentally induced increase in temperature, surprisingly adults were more resistant in warmer condition to Pb toxicity. In the summer adults were less resistant to Cu toxicity (96 h-LC 50 = 175 μg L −1 ) than in the winter (251 μg L −1 ). An opposite trend was observed for the Pb toxicity on adults between summer and winter (96 h-LC 50 of 742 vs 471 μg L −1 , respectively). Larvae displayed a slightly higher sensitivity to Cu and Pb than adults. An experimentally induced 3 °C increase in temperature above ambient decreased larval resistance to Cu and Pb toxicity by 23–30% (96 h-LC 50 of 167 vs 129 μg Cu L −1 and 681 vs 462 μg Pb L −1 ). Our data support the paradigm that upward excursions in temperature influence physiological processes in corals that play key roles in regulating metal toxicity. These influences are more pronounced in larva versus adult corals. These findings are important when contextualized climate change-driven warming in the oceans and highlight that predictions of ecological outcomes to metal pollutants will be improved by considering environmental context and the life stages of organism under study.

Environmental Pollution↗

Reproductive success and nest attentiveness of mallard ducks fed Aroclor 1254

A dietary dosage of 25 ppm Aroclor 1254 fed to nine-month-old mallards Anas platyrhynchos for at least a month before egg-laying had no detrimental effect on reproductive success or nest attentiveness when hens were allowed to incubate their own eggs. The treatment caused no effect on number of hens laying, date of first egg laid or clutch size. Fertility of eggs was greater among Aroclor-treated birds (87·7%) than among controls (73·2%). Aroclor may have stimulated males to come into reproductive condition sooner than controls. Hatching of fertile eggs and survival of ducklings to 3 weeks of age were similar in treated and control groups. The number of times off the nest per day and total time off the nest per day were the same for control and Aroclor-treated hens in days 14–17 of incubation.

Environmental Pollution (Series A)↗

Mercury and other element exposure in tree swallows nesting at low pH and neutral pH lakes in northern Wisconsin USA

The primary objective of this study was to determine whether tree swallows ( Tachycineta bicolor ) demonstrate similar responses to lake pH and mercury (Hg) contamination in northern Wisconsin as do common loons ( Gavia immer ). Similar to common loons, Hg concentrations in the blood of tree swallow nestlings were higher, Hg concentrations in eggs tended to be higher, and egg size tended to be smaller at low (<6.2) pH lakes. In contrast to common loons, tree swallow nestling production was not lower at low pH lakes. Based on modeling associations, Hg concentrations in tree swallow eggs and nestling blood can be used to predict Hg concentrations in common loons without the invasive or destructive sampling of loons. Mean concentrations of cadmium, manganese, and mercury in nestling livers were higher at low pH lakes than neutral pH lakes. Concentrations of cadmium, chromium, mercury, selenium, and zinc were not at toxic levels.

Wisconsin↗

Floristic summary of plant species in the air pollution literature

A floristic summary and analysis was performed on a list of the plant species that have been studied for the effects of gaseous and chemical air pollutants on vegetation in order to compare the species with the flora of North America north of Mexico. The scientific names of 2081 vascular plant species were extracted from almost 4000 journal articles stored in two large literature databases on the effects of air pollutants on plants. Three quarters of the plant species studied occur in North America, but this was only 7% of the total North American flora. Sixteen percent and 56% of all North American genera and families have been studied. The most studied genus is Pinus with 70% of the North American species studied, and the most studied family is the grass family, with 12% of the species studied. Although Pinus is ranked 86th in the North American flora, the grass family is ranked third, indicating that representation at the family level is better than at the genus level. All of the top ten families in North America are represented in the top 20 families in the air pollution effects literature, but only one genus ( Lupinus ) in the top ten genera in North America is represented in the top thirteen genera in the air pollution literature.

Environmental Pollution↗

Inhibition of hydroxyl radical reaction with aromatics by dissolved natural organic matter

Reaction of aromatic compounds with hydroxyl radical is inhibited by dissolved natural organic matter (NOM). The degree of inhibition is significantly greater than that expected based on a simple model in which aromatic compound molecules bound to NOM are considered to be unreactive. In this study, hydroxyl radical was produced at steady-state concentrations using Fenton chemistry (H2O2 + Fe2+ ??? Fe3+ + HO- + HO??). Suwannee River fulvic acid and humic acid were used as NOM. The most likely mechanism for the observed inhibition is that hydroxyl radical formation occurs in microenvironmental sites remote from the aromatic compounds. In addition to changes in kinetics, pyrene hydroxyl radical reaction also exhibited a mechanistic change in the presence of fulvic acid. The mechanism changed from a reaction that was apparently firstorder in pyrene to one that was apparently secondorder in pyrene, indicating that pyrene self-reaction may have become the dominant mechanism in the presence of fulvic acid. Dissolved NOM causes significant changes in the rate and mechanism of hydroxyl radical degradation of aromatic compounds. Consequently, literature rate constants measured in pure water will not be useful for predicting the degradation of pollutants in environmental systems. The kinetic and mechanistic information in this study will be useful for developing improved degradation methods involving Fenton chemistry.Reaction of aromatic compounds with hydroxyl radical is inhibited by dissolved natural organic matter (NOM). The degree of inhibition is significantly greater than that expected based on a simple model in which aromatic compounds molecules bounds to NOM are considered to be unreactive. In this study, hydroxyl radical was produced at steady-state concentrations using Fenton chemistry (H2O2 + Fe2+ ??? Fe3+ + HO- + HO??). Suwannee River fulvic acid and humic acid were used as NOM. The most likely mechanisms for the observed inhibition is that hydroxyl radical formation occurs in microenvironmental sites remote from the aromatic compounds. In addition to changes in kinetics, pyrene hydroxyl radical reaction also exhibited a mechanistic change in the presence of fulvic acid. The mechanism changed from a reaction that was apparently first-order in pyrene to one that was apparently second-order in pyrene, indicating that pyrene self-reaction may have become the dominant mechanism in the presence of fulvic acid. Dissolved NOM causes significant changes in the rate and mechanism of hydroxyl radical degradation of aromatic compounds. Consequently, literature rate constants measured in pure water will not be useful for predicting the degradation of pollutants in environmental systems. The kinetic and mechanistic information in this study will be useful for developing improved degradation methods involving Fenton chemistry.

Environmental Science & Technology↗

The WOAH global wildlife health collaborating centre network (WOAH-WildNet): A coordinated and transformative approach to global wildlife health challenges

Wildlife health is integral to functioning, complex ecosystems [ 1 ], directly and indirectly influencing the health of people, animals, plants, and the environment [ 2 – 4 ]. Healthy wildlife populations are essential for ecosystem services and are at the heart of the One Health approach [ 3 , 4 ], which aims to sustainably balance and optimize the health of people, animals, and ecosystems through multisectoral and transdisciplinary collaboration [ 5 ]. Despite its importance, wildlife health initiatives often operate in silos, limiting capacity to address transboundary threats such as emerging diseases, pollution, and environmental changes. Anthropogenic changes, including habitat loss, degradation, fragmentation, and unsustainable harvesting, exacerbate wildlife health challenges [6–9]. These pressures disrupt species biology and alter host-pathogen dynamics [10–12], underscoring the importance of coordinated collective action in addressing harmful effects on the health of wild animals. While local conservation efforts are vital, long-term success in safeguarding biodiversity requires a unified, global network. For instance, without harmonized surveillance and response systems, individual institutions cannot effectively track pathogens across borders or share diagnostic capabilities. The World Organisation for Animal Health (WOAH) Collaborating Centre Network for Wildlife Health—WOAH-WildNet—was established to bridge these gaps. By fostering global collaboration, sharing resources, and enabling data exchange, WOAH-WildNet provides a transformative, systems-based approach to wildlife health, managing risks, and enhancing ecosystem resilience. Central to this mission is breaking down silos to promote intersectoral coordinated responses to complex wildlife health challenges.

PLOS Sustainability and Transformation↗

Ranking terrestrial vertebrate species for utility in biomonitoring and vulnerability to environmental contaminants

The measurement of contaminant tissue concentrations or exposure-related effects in biota has been used extensively to monitor pollution and environmental health. Terrestrial vertebrates have historically been an important group of species in such evaluations, not only because many are excellent sentinels of environmental contamination, but also because they are valued natural resources in their own right that may be adversely affected by toxicant exposure. Selection of appropriate vertebrates for biomonitoring studies frequently relies on expert opinion, although a few rigorous schemes are in use for predicting vulnerability of birds to the adverse effects of petroleum crude oil. A Utility Index that ranks terrestrial vertebrate species as potential sentinels of contaminants in a region, and a Vulnerability Index that assesses the threat of specific groups of contaminants to these species , have been developed to assist decision makers in risk assessments of persistent organic pollutants, cholinesterase-inhibiting pesticides, petroleum crude oil, mercury, and lead shot. Twenty-five terrestrial vertebrate species commonly found in Atlantic Coast estuarine habitat (Rattner et al. 2001a) were ranked for their utility as biomonitors of contamination and their vulnerability to pollutants in this region. No single species , taxa, or class of vertebrates was found to be an ideal sentinel for all groups of contaminants . Although birds have overwhelmingly been used to monitor contaminants compared to other terrestrial vertebrate classes, the nonmigratory nature and dietary habits of the snapping turtle and mink consistently resulted in ranking these species as excellent sentinels as well. Vulnerability of Atlantic Coast populations of these species varied considerably among groups of contaminants . Usually a particular species was found to be at high risk to only one or two groups of contaminants , although a noteworthy exception is the bald eagle, which is highly vulnerable to all five of the contaminant groups examined. This index could be further enhanced by generation of additional comparative toxicity data to facilitate interspecific extrapolations. The Utility and Vulnerability Indices have application to many types of habitats in addition to estuaries and are of value to natural resource and risk managers that routinely conduct local, regional, or national environmental quality assessments.

Archives of Environmental Contamination and Toxico↗

Spatial distribution of tropospheric ozone in western Washington, USA

We quantified the distribution of tropospheric ozone in topographically complex western Washington state, USA (total area a??6000 km2), using passive ozone samplers along nine river drainages to measure ozone exposure from near sea level to high-elevation mountain sites. Weekly average ozone concentrations were higher with increasing distance from the urban core and at higher elevations, increasing a mean of 1.3 ppbv per 100 m elevation gain for all mountain transects. Weekly average ozone concentrations were generally highest in Cascade Mountains drainages east and southeast of Seattle (maximum=55a??67 pbv) and in the Columbia River Gorge east of Portland (maximum=59 ppbv), and lowest in the western Olympic Peninsula (maximum=34 ppbv). Higher ozone concentrations in the Cascade Mountains and Columbia River locations downwind of large cities indicate that significant quantities of ozone and ozone precursors are being transported eastward toward rural wildland areas by prevailing westerly winds. In addition, temporal (week to week) variation in ozone distribution is synchronous within and between all drainages sampled, which indicates that there is regional coherence in air pollution detectable with weekly averages. These data provide insight on large-scale spatial variation of ozone distribution in western Washington, and will help regulatory agencies optimize future monitoring networks and identify locations where human health and natural resources could be at risk.

Environmental Pollution↗

Sequestration of microfibers and other microplastics by green algae, Cladophora, in the US Great Lakes

Daunting amounts of microplastics are present in surface waters worldwide. A main category of microplastics is synthetic microfibers, which originate from textiles. These microplastics are generated and released in laundering and are discharged by wastewater treatment plants or enter surface waters from other sources. The polymers that constitute many common synthetic microfibers are mostly denser than water, and eventually settle out in aquatic environments. The interaction of these microfibers with submerged aquatic vegetation has not been thoroughly investigated but is potentially an important aquatic sink in surface waters. In the Laurentian Great Lakes, prolific growth of macrophytic Cladophora creates submerged biomass with a large amount of surface area and the potential to collect and concentrate microplastics. To determine the number of synthetic microfibers in Great Lakes Cladophora , samples were collected from Lakes Erie and Michigan at multiple depths in the spring and summer of 2018. After rinsing and processing the algae, associated synthetic microfibers were quantified. The average loads of synthetic microfibers determined from the Lake Erie and Lake Michigan samples were 32,000 per kg (dry weight (dw)) and 34,000 per kg (dw), respectively, 2–4 orders of magnitude greater than loads previously reported in water and sediment. To further explore this sequestration of microplastics, fresh and aged Cladophora were mixed with aqueous mixtures of microfibers or microplastic in the laboratory to simulate pollution events. Microscopic analyses indicated that fresh Cladophora algae readily interacted with microplastics via adsorptive forces and physical entanglement. These interactions mostly cease upon algal senescence, with an expected release of microplastics in benthic sediments. Collectively, these findings suggest that synthetic microfibers are widespread in Cladophora algae and the affinity between microplastics and Cladophora may offer insights for removing microplastic pollution. Macroalgae in the Laurentian Great Lakes contain high loads of synthetic microfibers, both entangled and adsorbed, which likely account for an important fraction of microplastics in these surface waters.

Michigan↗

Diversity, composition, and geographical distribution of microbial communities in California salt marsh sediments

The Pacific Estuarine Ecosystem Indicators Research Consortium seeks to develop bioindicators of toxicant-induced stress and bioavailability for wetland biota. Within this framework, the effects of environmental and pollutant variables on microbial communities were studied at different spatial scales over a 2-year period. Six salt marshes along the California coastline were characterized using phospholipid fatty acid (PLFA) analysis and terminal restriction fragment length polymorphism (TRFLP) analysis. Additionally, 27 metals, six currently used pesticides, total polychlorinated biphenyls and polycyclic aromatic hydrocarbons, chlordanes, nonachlors, dichlorodiphenyldichloroethane, and dichlorodiphenyldichloroethylene were analyzed. Sampling was performed over large (between salt marshes), medium (stations within a marsh), and small (different channel depths) spatial scales. Regression and ordination analysis suggested that the spatial variation in microbial communities exceeded the variation attributable to pollutants. PLFA analysis and TRFLP canonical correspondence analysis (CCA) explained 74 and 43% of the variation, respectively, and both methods attributed 34% of the variation to tidal cycles, marsh, year, and latitude. After accounting for spatial variation using partial CCA, we found that metals had a greater effect on microbial community composition than organic pollutants had. Organic carbon and nitrogen contents were positively correlated with PLFA biomass, whereas total metal concentrations were positively correlated with biomass and diversity. Higher concentrations of heavy metals were negatively correlated with branched PLFAs and positively correlated with methyl- and cyclo-substituted PLFAs. The strong relationships observed between pollutant concentrations and some of the microbial indicators indicated the potential for using microbial community analyses in assessments of the ecosystem health of salt marshes. Copyright ?? 2006, American Society for Microbiology. All Rights Reserved.

Applied and Environmental Microbiology↗

Synthesis of isotopically modified ZnO nanoparticles and their potential as nanotoxicity tracers

Understanding the behavior of engineered nanoparticles in the environment and within organisms is perhaps the biggest obstacle to the safe development of nanotechnologies. Reliable tracing is a particular issue for nanoparticles such as ZnO, because Zn is an essential element and a common pollutant thus present at elevated background concentrations. We synthesized isotopically enriched (89.6%) with a rare isotope of Zn ( 67 Zn) ZnO nanoparticles and measured the uptake of 67 Zn by L. stagnalis exposed to diatoms amended with the particles. Stable isotope technique is sufficiently sensitive to determine the uptake of Zn at an exposure equivalent to lower concentration range (<15 μg g −1 ). Without a tracer, detection of newly accumulated Zn is significant at Zn exposure concentration only above 5000 μg g −1 which represents some of the most contaminated Zn conditions. Only by using a tracer we can study Zn uptake at a range of environmentally realistic exposure conditions.

Environmental Pollution↗

Limitations on analysis of small particles with an electron probe: Pollution studies

Recent literature concerning the size and composition of airborne lead particles in automobile exhaust emission determined by electron microprobe analysis reports fourteen distinct lead compounds. Particle sizes reported were from 0·2 μm to 2 μm in diameter. The determination of chemical formulae for compounds requires quantitative elemental data for individual particles. It was also assumed that the lead-bearing particles analysed were solid (specifically non-porous or non-fluffy) compounds which occurred as discrete (non-aggregate) particles. Intensity data obtained in our laboratory from the excited volume in a 1 μm-diameter sphere of solid lead chloride indicate insufficient precision and sensitivity to obtain chemical formulae as reported in the literature for exhaust emission products.

Environmental Pollution↗

Rapid regional recovery from sulfate and nitrate pollution in streams of the western Czech Republic - Comparison to other recovering areas

Hydrochemical changes between 1991 and 2001 were assessed based on two synoptic stream surveys from the 820-km2 region of the Slavkov Forest and surrounding area, western Czech Republic. Marked declines of sulfate, nitrate, chloride, calcium and magnesium in surface waters were compared with other areas of Europe and North America recovering from acidification. Declines of sulfate concentration in the Slavkov Forest (-30 ??eq L-1 yr-1) were more dramatic than declines reported from other sites. However, these dramatic declines of strong acid anions did not generate a widespread increase of stream water pH in the Slavkov Forest. Only the most acidic streams experienced a slight increase of pH by 0.5 unit. An unexpected decline of stream water pH occurred in slightly alkaline streams. ?? 2004 Elsevier Ltd. All rights reserved.

Environmental Pollution↗

Accumulation and bioconcentration of polycyclic aromatic hydrocarbons in a nearshore estuarine environment near a Pensacola (Florida) creosote contamination site

Long-term accumulation of creosote wastes at a wood-preserving facility near Pensacola, Florida, has produced high levels of organic contamination of groundwaters near Pensacola Bay. Impacts of this contamination on the nearshore environment of the bay were examined by analysis of water, sediment and tissues of two mollusc species. One of the species (Thais haemastoma) was native to the study area. Individuals of the other test species (Crassostrea virginica) were placed in cages at the test sites for a 6-week period. Contamination at the nearshore estuarine sites was assessed by comparison to a control site in an uncontaminated area of the bay, as well as a small stream which forms a direct surface-water link between the creosote storage ponds and the bay. The study focused on polycyclic aromatic hydrocarbons (PAH), the primary components of creosote. Very little PAH in water or in the surface layer of estuarine sediments was detected, despite heavy pollution of the stream sediments. This is attributed to various degradation processes which attack the PAH compounds once they discharge into the estuary, and to the likelihood of intermittent and localised release of contaminants to the estuary. Examination of sediment cores and mollusc tissues, which provide a record integrated over time and space, revealed some accumulation of a few PAH, notably fluoranthene, pyrene, benzo(a)anthracene, chrysene and phenanthrene. In the sediments, the highest concentrations of these compounds appeared below the surface, within a depth range of 8-13 cm. Bioaccumulation of fluoranthene, pyrene and phenanthrene in both mollusc species was up to ten times greater at test sites than at the control site. This contrasts with naphthalene, the bioaccumulation of which was no greater at test sites than at the control site. These differences in bioaccumulation factors relate to structural chemistry of the compounds which control their solubility, bioavailability, susceptibility to degradation and capacity for depuration by the organism.Long-term accumulation of creosote wastes at a wood-preserving facility near Pensacola, Florida, has produced high levels of organic contamination of groundwaters near Pensacola Bay. Impacts of this contamination on the nearshore environment of the bay were examined by analysis of water, sediment and tissues of two mollusc species. Very little PAH in water or in the surface layer of estuarine sediments was detected, despite heavy pollution of the stream sediments. This is attributed to various degradation processes which attack the PAH compounds once they discharge into the estuary, and to the likelihood of intermittent and localized release of contaminants to the estuary. There was some accumulation of a few PAH, notably fluoranthene, pyrene, benzo(a)anthracene, chrysene and phenanthrene. In the sediments, the highest concentrations of these compounds appeared below the surface, within a depth range of 8-13 cm. Bioaccumulation of fluoranthene, pyrene and phenanthrene in both mollusc species was up to ten times greater at test sites than at the control site. Differences in bioaccumulation factors relate to structural chemistry of the compounds which control their solubility, bioavailability, susceptibility to degradation and capacity for depuration by the organism.

Florida↗

Concentrations of chromium, manganese, and lead in air and in avian eggs

The expansion of urbanization introduces air pollution to wildlife areas. Some metal contaminants occurring in concentrations too small to have any measurable impact on adult birds may seriously affect embryos that are more sensitive to contaminants than the adult. Chromium, manganese, and lead are toxic and can be passed from the hen to the egg. This study relates the concentrations of these metals in eggs to their concentrations in air in three cities. Rock dove eggs were sampled and air pollution records were examined in the California cities of Riverside, Los Angeles, and San Francisco. The eggs from San Francisco did not differ from those of Los Angeles in lead concentration but the air did differ. The eggs collected in Los Angeles in 1998 had concentrations of chromium greater than in those from Riverside and from Los Angeles 1999 but the air had concentrations of chromium that did not differ among those three collections. Concentrations of manganese did not differ among the eggs but did differ among the air samples of the three cities. Exposures of embryos to chromium and manganese in this study were not at levels warranting concern. Although the concentration at which lead in eggs impairs avian health is not established, the highest concentrations found in this study exceed estimated safe concentrations. There is no indication that embryo exposure is directly related to atmospheric levels of these metals in the cities of this study.

California↗