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At least 865 records · Page 48Linked to original sources
USGS2014

Remote sensing of Sonoran Desert vegetation structure and phenology with ground-based LiDAR

Long-term vegetation monitoring efforts have become increasingly important for understanding ecosystem response to global change. Many traditional methods for monitoring can be infrequent and limited in scope. Ground-based LiDAR is one remote sensing method that offers a clear advancement to monitor vegetation dynamics at high spatial and temporal resolution. We determined the effectiveness of LiDAR to detect intra-annual variability in vegetation structure at a long-term Sonoran Desert monitoring plot dominated by cacti, deciduous and evergreen shrubs. Monthly repeat LiDAR scans of perennial plant canopies over the course of one year had high precision. LiDAR measurements of canopy height and area were accurate with respect to total station survey measurements of individual plants. We found an increase in the number of LiDAR vegetation returns following the wet North American Monsoon season. This intra-annual variability in vegetation structure detected by LiDAR was attributable to a drought deciduous shrub Ambrosia deltoidea, whereas the evergreen shrub Larrea tridentata and cactus Opuntia engelmannii had low variability. Benefits of using LiDAR over traditional methods to census desert plants are more rapid, consistent, and cost-effective data acquisition in a high-resolution, 3-dimensional context. We conclude that repeat LiDAR measurements can be an effective method for documenting ecosystem response to desert climatology and drought over short time intervals and at detailed-local spatial scale.

Arizona↗
USGS2017

Fine-resolution repeat topographic surveying of dryland landscapes using UAS-based structure-from-motion photogrammetry: Assessing accuracy and precision against traditional ground-based erosion measurements

Structure-from-motion (SfM) photogrammetry from unmanned aerial system (UAS) imagery is an emerging tool for repeat topographic surveying of dryland erosion. These methods are particularly appealing due to the ability to cover large landscapes compared to field methods and at reduced costs and finer spatial resolution compared to airborne laser scanning. Accuracy and precision of high-resolution digital terrain models (DTMs) derived from UAS imagery have been explored in many studies, typically by comparing image coordinates to surveyed check points or LiDAR datasets. In addition to traditional check points, this study compared 5 cm resolution DTMs derived from fixed-wing UAS imagery with a traditional ground-based method of measuring soil surface change called erosion bridges. We assessed accuracy by comparing the elevation values between DTMs and erosion bridges along thirty topographic transects each 6.1 m long. Comparisons occurred at two points in time (June 2014, February 2015) which enabled us to assess vertical accuracy with 3314 data points and vertical precision (i.e., repeatability) with 1657 data points. We found strong vertical agreement (accuracy) between the methods (RMSE 2.9 and 3.2 cm in June 2014 and February 2015, respectively) and high vertical precision for the DTMs (RMSE 2.8 cm). Our results from comparing SfM-generated DTMs to check points, and strong agreement with erosion bridge measurements suggests repeat UAS imagery and SfM processing could replace erosion bridges for a more synoptic landscape assessment of shifting soil surfaces for some studies. However, while collecting the UAS imagery and generating the SfM DTMs for this study was faster than collecting erosion bridge measurements, technical challenges related to the need for ground control networks and image processing requirements must be addressed before this technique could be applied effectively to large landscapes.

Remote Sensing↗
USGS2025

A real-time fish detection system for partially dewatered fish to support selective fish passage

Recent advances in fish transportation technologies and deep machine learning-based fish classification have created an opportunity for real-time, autonomous fish sorting through a selective passage mechanism. This research presents a case study of a novel application that utilizes deep machine learning to detect partially dewatered fish exiting an Archimedes Screw Fish Lift (ASFL). A MobileNet SSD model was trained on images of partially dewatered fish volitionally passing through an ASFL. Then, this model was integrated with a network video recorder to monitor video from the ASFL. Additional models were also trained using images from a similar fish scanning device to test the feasibility of this approach for fish classification. Open source software and edge computing design principles were employed to ensure that the system is capable of fast data processing. The findings from this research demonstrate that such a system integrated with an ASFL can support real-time fish detection. This research contributes to the goal of automated data collection in a selective fish passage system and presents a viable path towards realizing optical fish sorting.

Michigan↗
USGS2011

Hyperkyphosis in longnose gar ( Lepisosteus osseus ) of North Central Texas

Two mature female longnose gar ( Lepisosteus osseus ) exhibiting severe hyperkyphotic spinal deformities were captured during the 2010 spring spawn at Lake Arrowhead, Clay County, Texas. Yet, despite their deformities and impaired motility, both longnose gar were in overall good condition. Hyperkyphosis in both longnose gar resulted from fused trunk vertebrae in the affected areas. Results of morphological examinations and computerized tomography (CT) scans showed no evidence of injury-induced responses and suggested a congenital or possibly environmentally induced aetiology for disruption of the normal vertebral segmentation process.

Texas↗
USGS2014

A new clarification method to visualize biliary degeneration during liver metamorphosis in sea lamprey ( Petromyzon marinus )

Biliary atresia is a rare disease of infancy, with an estimated 1 in 15,000 frequency in the southeast United States, but more common in East Asian countries, with a reported frequency of 1 in 5,000 in Taiwan. Although much is known about the management of biliary atresia, its pathogenesis is still elusive. The sea lamprey ( Petromyzon marinus ) provides a unique opportunity to examine the mechanism and progression of biliary degeneration. Sea lamprey develop through three distinct life stages: larval, parasitic, and adult. During the transition from larvae to parasitic juvenile, sea lamprey undergo metamorphosis with dramatic reorganization and remodeling in external morphology and internal organs. In the liver, the entire biliary system is lost, including the gall bladder and the biliary tree. A newly-developed method called “CLARITY” was modified to clarify the entire liver and the junction with the intestine in metamorphic sea lamprey. The process of biliary degeneration was visualized and discerned during sea lamprey metamorphosis by using laser scanning confocal microscopy. This method provides a powerful tool to study biliary atresia in a unique animal model.

Journal of Visualized Experiments↗
USGS2024

Visual interpretation of high-resolution aerial imagery: A tool for land managers

Remotely sensed imagery from various collection platforms (e.g., satellites, crewed and uncrewed aircraft) are used by biologists and other conservation personnel to support management activities ranging from monitoring invasive species to assessing land cover and vegetation characteristics. Although remote sensing–based vegetation indices and models have been developed and used for some management applications, straightforward visual interpretation of imagery by on-the-ground personnel may be a pragmatic approach for obtaining time-sensitive and spatially relevant information to support and guide local management activities. Our primary objective was to qualitatively assess our ability to identify patches of target invasive plant species based on simple visual interpretation of high-resolution aerial imagery. We also sought to compare the high-resolution imagery to widely available imagery (e.g., National Agriculture Imagery Program) to determine the efficacy of each for assessing vegetation communities and land-cover features in support of management activities. To accomplish these objectives, we obtained high-resolution imagery and visually scanned and assessed the imagery by using standard geographic information system software. We were able to differentiate patches of crownvetch Securigera varia (L.) Lassen and wild parsnip Pastinaca sativa L., but not spotted knapweed Centaurea stoebe L. or leafy spurge Euphorbia esula L. The relative success in identifying these species had a relationship to plant characteristics (e.g., flower color and morphology, height), time of year (phenology), patch size and density, and potentially site characteristics such density of the underlying vegetation (e.g., grasses), substrate color characteristics (i.e., color contrast with flowers), and physical disturbance. Our straightforward, qualitative assessment suggests that visual interpretation of high-resolution imagery, but not some lower-resolution imagery, may be an efficient and effective tool for supporting local invasive species management through activities such as monitoring known patches, identifying undetected infestations, assessing management actions, guiding field work, or prioritizing on-the-ground monitoring activities.

Journal of Fish and Wildlife Management↗
USGS2013

Thermogenic gases in near-surface sediments of Norton Sound, Alaska

A plume of hydrocarbon gases, assumed to be of thermogenic origin based on chemical compositions, has been noted by others in the water column of Norton Sound about 40 km south of Nome, Alaska. We used detailed geophysical transects, side-scan sonar, underwater television, and chromatographic analyses of gases in near-surface sediments to define a probable source area of hydrocarbon gases at the southern apex of the water plume epicenter. Geophysical, geotechnical, and geochemical evidence together indicate that hydrocarbon gases of subsurface, thermogenic origin apparently migrate into the near surface sediments along a fault zone. Subbottom reflector terminations on continuous seismic profiles outline a large zone of anomalous acoustic responses about 9 km in diameter and at 100-m depth that may be caused by a subsurface accumulation of gas. Gas migration from the accumulation to the surface sediment is indicated by smaller zones of reflector terminations observed in high-resolution profiles and by seafloor craters seen on underwater television at one station. The presence of gas-charged surface sediment also is suggested by low percent pore water saturation, greater penetration of the penetrometer, and more rapid penetration of the vibracorer in sediment at the station with near-surface acoustic anomalies and seafloor craters. Analyses of hydrocarbon gases in sediments from a 1.6-m vibracore taken at this station showed unusually high concentrations of hydrocarbon gases heavier than methane; the ethane, propane, n-butane, and isobutane were 76, 4, 6, and 52 times greater than in other near-surface sediments in this region, and also significant quantities of gasoline-range hydrocarbons were present. The gas composition and presence in near-surface sediments above a thick underlying section with acoustic anomalies points to the possibility of petroleum at depth in this region. Our work indicates that surface and near-surface studies of the continental shelf may contribute significantly to petroleum-resource evaluations in addition to defining areas of potential hazard from gas-charged sediment.

Alaska↗
USGS2003

Exopolymer microenvironments of microbial flora: Multiple and interactive effects on trophic relationships

Microbial cells in natural environments are often encased in different types of exopolymer secretions (EPS), ranging from tight capsules surrounding individual cells to the looser slime matrices of biofilms. The different physical and chemical properties of exopolymers could have secondary effects on trophic interactions between microbial cells and consumer animals. Laboratory studies showed that capsule EPS is significantly less digestible to consumers than slime EPS, even when extracted from the same bacterial strain. Bacterial cells with EPS capsules are less efficiently digested than noncapsuled cells, suggesting that capsules protect against digestion. Follow-up experiments determined that polysaccharide-rich fractions of slime EPS are absorbed with very high efficiencies while protein portions, which are more abundant in capsular polymers, are absorbed relatively poorly. Another series of experiments showed that dissolved organic matter (DOM), when adsorbed directly to the mineralogical portions of sediment particles, is available to deposit feeders. However, the further presence of an exopolymer coating on sediments more than doubled the bioavailability of adsorbed DOM to the consumer. Observations using cold-stage scanning electron microscopy indicated that exopolymer microenvironments are a common feature of natural marine sediments. Microbial exopolymers range from easily digestible carbon sources to relatively refractory ones that effectively protect some microbial cells from consumer digestion. Exopolymer microenvironments may also make recently adsorbed DOM highly accessible to particle-ingesting animals.

Limnology and Oceanography↗
USGS2021

Synoptic analysis of a decade of daily measurements of SO2 emission in the troposphere from volcanoes of the global ground-based Network for Observation of Volcanic and Atmospheric Change

Volcanic plumes are common and far-reaching manifestations of volcanic activity during and between eruptions. Observations of the rate of emission and composition of volcanic plumes are essential to recognize and, in some cases, predict the state of volcanic activity. Measurements of the size and location of the plumes are important to assess the impact of the emission from sporadic or localized events to persistent or widespread processes of climatic and environmental importance. These observations provide information on volatile budgets on Earth, chemical evolution of magmas, and atmospheric circulation and dynamics. Space-based observations during the last decades have given us a global view of Earth's volcanic emission, particularly of sulfur dioxide ( SO 2 ). Although none of the satellite missions were intended to be used for measurement of volcanic gas emission, specially adapted algorithms have produced time-averaged global emission budgets. These have confirmed that tropospheric plumes, produced from persistent degassing of weak sources, dominate the total emission of volcanic SO 2 . Although space-based observations have provided this global insight into some aspects of Earth's volcanism, it still has important limitations. The magnitude and short-term variability of lower-atmosphere emissions, historically less accessible from space, remain largely uncertain. Operational monitoring of volcanic plumes, at scales relevant for adequate surveillance, has been facilitated through the use of ground-based scanning differential optical absorption spectrometer (ScanDOAS) instruments since the beginning of this century, largely due to the coordinated effort of the Network for Observation of Volcanic and Atmospheric Change (NOVAC). In this study, we present a compilation of results of homogenized post-analysis of measurements of SO 2 flux and plume parameters obtained during the period March 2005 to January 2017 of 32 volcanoes in NOVAC. This inventory opens a window into the short-term emission patterns of a diverse set of volcanoes in terms of magma composition, geographical location, magnitude of emission, and style of eruptive activity. We find that passive volcanic degassing is by no means a stationary process in time and that large sub-daily variability is observed in the flux of volcanic gases, which has implications for emission budgets produced using short-term, sporadic observations. The use of a standard evaluation method allows for intercomparison between different volcanoes and between ground- and space-based measurements of the same volcanoes. The emission of several weakly degassing volcanoes, undetected by satellites, is presented for the first time. We also compare our results with those reported in the literature, providing ranges of variability in emission not accessible in the past. The open-access data repository introduced in this article will enable further exploitation of this unique dataset, with a focus on volcanological research, risk assessment, satellite-sensor validation, and improved quantification of the prevalent tropospheric component of global volcanic emission.

Earth System Science Data↗
USGS2026

CoralCache: A virtual coral core repository for transparent and reproducible annual growth rate analyses

As science fields enter the Big Data revolution, open-access repositories are essential for addressing larger-scale questions than are possible for single researchers by making data findable, accessible, interoperable, and reusable (FAIR). Furthermore, transparent data and code are increasingly important for reproducible research, especially for data types that inherently require subjective human interpretations. These ideas are applicable to coral sclerochronology, as the field has long been characterized by individual researchers collecting and analyzing coral skeletal cores from their study sites without substantial data sharing or archiving of the core images to meet FAIR principles. Here, we present CoralCache, a virtual coral core repository that not only archives image datasets (i.e., digitized X-rays and computed tomography scans), but also observer interpretations of the density banding patterns. CoralCache is linked to a graphical user interface, CoralCT, which together offer a way forward for coral growth rate analysis that is reproducible and collaborative. The data organization systems presented here could also be readily applied to related archives such as tree rings or bivalve shells.

Earth System Science Data↗
USGS2013

Large scale snow water status monitoring: Comparison of different snow water products in the upper Colorado basins

We illustrate the ability to monitor the status of snow water content over large areas by using a spatially distributed snow accumulation and ablation model that uses data from a weather forecast model in the upper Colorado Basin. The model was forced with precipitation fields from the National Weather Service (NWS) Multi-sensor Precipitation Estimator (MPE) and the Tropical Rainfall Measuring Mission (TRMM) data-sets; remaining meteorological model input data were from NOAA's Global Forecast System (GFS) model output fields. The simulated snow water equivalent (SWE) was compared to SWEs from the Snow Data Assimilation System (SNODAS) and SNOwpack TELemetry system (SNOTEL) over a region of the western US that covers parts of the upper Colorado Basin. We also compared the SWE product estimated from the special sensor microwave imager (SSM/I) and scanning multichannel microwave radiometer (SMMR) to the SNODAS and SNOTEL SWE data-sets. Agreement between the spatial distributions of the simulated SWE with MPE data was high with both SNODAS and SNOTEL. Model-simulated SWE with TRMM precipitation and SWE estimated from the passive microwave imagery were not significantly correlated spatially with either SNODAS or the SNOTEL SWE. Average basin-wide SWE simulated with the MPE and the TRMM data were highly correlated with both SNODAS ( r = 0.94 and r = 0.64; d.f. = 14 – d.f. = degrees of freedom) and SNOTEL ( r = 0.93 and r = 0.68; d.f. = 14). The SWE estimated from the passive microwave imagery was significantly correlated with the SNODAS SWE ( r = 0.55, d.f. = 9, p = 0.05) but was not significantly correlated with the SNOTEL-reported SWE values ( r = 0.45, d.f. = 9, p = 0.05).The results indicate the applicability of the snow energy balance model for monitoring snow water content at regional scales when coupled with meteorological data of acceptable quality. The two snow water contents from the microwave imagery (SMMR and SSM/I) and the Utah Energy Balance forced with the TRMM precipitation data were found to be unreliable sources for mapping SWE in the study area; both data sets lacked discernible variability of snow water content between sites as seen in the SNOTEL and SNODAS SWE data. This study will contribute to better understanding the adequacy of data from weather forecast models, TRMM, and microwave imagery for monitoring status of the snow water content.

Colorado, Utah, Wyoming↗
USGS2025

Geochemistry of quartz and fluid inclusions associated with gold, tungsten, and antimony in the Stibnite-Yellow Pine district, Idaho

Deposits in the historic Stibnite-Yellow Pine district in east-central Idaho were mined for Ag, Au, Hg, Sb, and W. Ore is hosted in breccia and fault zones along NE-striking faults that transect the Atlanta lobe of the Late Cretaceous Idaho batholith and metamorphosed Neoproterozoic to lower Paleozoic rocks. Geochronological studies have delineated five superimposed and zoned hydrothermal events in the mining district which range in age from Late Cretaceous to the Eocene. They formed the following consecutive assemblages: (1) vein and disseminated pre-ore milky quartz (± calcite), muscovite, and brassy pyrite with minor molybdenite and base metal sulfides; (2) vein and disseminated quartz, calcite, sericite, auriferous-pyrite, and arsenopyrite; (3) potassium feldspar, quartz, dolomite, and calcite with vein and disseminated scheelite ± pyrite and arsenopyrite; (4) Au-Ag veins that contain adularia; and (5) breccia, vein, and disseminated quartz, calcite, and stibnite with minor Ag sulfosalts and distal zones of cinnabar and realgar at Fern and Hermes mines. In this study, the pressure, temperature, and composition of ore-forming fluids were measured in Au-, W-, and Sb-bearing samples collected from drill core and surface exposures. Using optical petrography, scanning electron microscopy cathodoluminescence (SEM-CL) and electron microprobe cathodoluminescence (EMPA-CL) imaging, as well as electron microprobe (EMP) and laser ablation-inductively coupled plasma-mass spectrometry trace element analyses, four major overprinting generations of hydrothermal quartz are defined. Fluid inclusion assemblages (FIAs) in each generation of quartz were characterized by microthermometry and laser Raman spectroscopy. Quartz and fluid inclusion paragenesis are as follows: (Qi) altered and overprinted Qi with no coeval FIAs that formed before or during event 1; (Q1) early veins as well as disseminated quartz with dull gray SEM-CL and H 2 O-NaCl-CO 2 FIAs that formed at minimum temperatures and pressures of 281° to 358°C and 1346 to 1789 bar during event 2 (Au); (Q2) vein and disseminated quartz, often euhedral, with H 2 O-NaCl ± CO 2 ± CH 4 FIAs that formed at minimum temperatures and pressures of 183° to 213°C and 72 to 602 bar during event 3 (W); and (Q3) euhedral and disseminated quartz with H 2 O-NaCl FIAs that formed at minimum temperature and pressures of 156° to 161°C and 5 to 6 bar during event 5 (Sb). Stibnite hosts H 2 O-NaCl FIAs trapped at minimum temperatures and pressures of 150° to 189°C and 5 to 12 bar that are similar to those of Q3-hosted FIAs. The quartz paragenetic history in the district follows a progressive temporal path from Tienriched quartz deposited by a relatively deep and higher temperature aqueous-carbonic fluid to a Li, Al, and Sb enriched quartz precipitated from a lower temperature aqueous fluid. Gases in fluid inclusion extracts from ore and gangue minerals were analyzed by mass spectrometry. Noble gas isotope analysis of fluid inclusion extracts revealed three signatures. Pre-ore Qi has R/R A (the isotopic ratio of the sample 3 He/ 4 He to atmospheric 3 He/ 4 He (1.384 × 10 −6 )) values that reflect the composition of the crustal melt-derived Atlanta lobe of the Idaho batholith. Quartz (Q1) and auriferous arsenian pyrite of hydrothermal event 2 have 20 Ne/ 4 He and R/R A values that plot along the air-crust mixing line, and these gases were likely introduced by metamorphic fluids derived from metasedimentary rocks. Stibnite with associated quartz (Q3 ± Q2) have 20 Ne/ 4 He and R/R A values that extend from the air-crust mixing line to the air-mantle mixing line, showing a shift from a crustal signature to an overprinting mantle signature. Late calcite has 20 Ne/ 4 He and R/R A values that extend from air toward the mantle end member, which suggests that magmatic volatiles condensed into meteoric groundwater late in the paragenesis. A mantle volatile source is also supported by high 20 Ne/ 22 Ne ratios. The Stibnite-Yellow Pine district is similar to other Au-W-Sb districts around the world in ore fluid chemistries, with late-stage stibnite being a product of cooling. The fluid pressure-temperature-composition (P-T-X) estimates and the crustal source of He during hydrothermal event 2 gold mineralization are characteristic of orogenic Au deposits, and the observed inclusion reequilibration textures indicate that trapped fluid inclusions were deformed during transitions between the brittle-ductile environment. Scheelite was deposited during event 3 with Q2 quartz and is overgrown by Q3 quartz and stibnite. Our interpretation of the quartz texture, chemistry, and fluid inclusion P-T-X data suggest that Au, W, and Sb mineralization occurred as hydrothermal fluids evolved from H 2 O-NaCl-CO 2 at >300°C and depths of ~4 to 6 km near the ductile to brittle transition to H 2 O-NaCl at ~150°C and epithermal depths as the Idaho batholith was exhumed. Geochronology indicates that from the Late Cretaceous to early Eocene, overprinting hydrothermal events formed deep orogenic Au, intermediate W, and shallow epithermal Au-Ag and Sb. Without comprehensive geochronologic constraints, our fluid inclusion data and textural observations could be interpreted as a single evolving hydrothermal system, as has been done in many other mining districts with similar mineralization.

Idaho↗
USGS2014

Evaluation of Landsat-7 SLC-off image products for forest change detection

Since July 2003, Landsat-7 ETM+ has been operating without the scan line corrector (SLC), which compensates for the forward motion of the satellite in the imagery acquired. Data collected in SLC-off mode have gaps in a systematic wedge-shaped pattern outside of the central 22 km swath of the imagery; however, the spatial and spectral quality of the remaining portions of the imagery are not diminished. To explore the continued use of Landsat-7 ETM+ SLC-off imagery to characterize change in forested environments, we compare the change detection results generated from a reference image pair (a 1999 Landsat-7 ETM+ image and a 2003 Landsat-5 TM image) with change detection results generated from the same 1999 Landsat-7 ETM+ image coupled with three different 2003 Landsat-7 SLC-off products: unremediated SLC-off (i.e., with gaps); histogram-based gap-filled; and segment-based gap-filled. The results are compared on both a pixel and polygon basis; on a pixel basis, the unremediated SLC-off product missed 35% of the change identified by the reference data, and the histogram- and segment-based gap-filled products missed 23% and 21% of the change, respectively. When using forest inventory polygons as a context for change (to reduce commission error), the amount of change missed was 31%, 14%, and 12% for the each of the unremediated, histogram-based gap-filled, and segment-based gap-filled products, respectively. Our results indicate that over the time period considered, and given the types and spatial distribution of change events within our study area, the gap-filled products can provide a useful data source for change detection in forested environments. The selection of which product to use is, however, very dependent on the nature of the application and the spatial configuration of change events. ?? 2008 Government of Canada.

Canadian Journal of Remote Sensing↗
USGS2014

Establishing the Antarctic Dome C community reference standard site towards consistent measurements from Earth observation satellites

Establishing satellite measurement consistency by using common desert sites has become increasingly more important not only for climate change detection but also for quantitative retrievals of geophysical variables in satellite applications. Using the Antarctic Dome C site (75°06′S, 123°21′E, elevation 3.2 km) for satellite radiometric calibration and validation (Cal/Val) is of great interest owing to its unique location and characteristics. The site surface is covered with uniformly distributed permanent snow, and the atmospheric effect is small and relatively constant. In this study, the long-term stability and spectral characteristics of this site are evaluated using well-calibrated satellite instruments such as the Moderate Resolution Imaging Spectroradiometer (MODIS) and Sea-viewing Wide Field-of-view Sensor (SeaWiFS). Preliminary results show that despite a few limitations, the site in general is stable in the long term, the bidirectional reflectance distribution function (BRDF) model works well, and the site is most suitable for the Cal/Val of reflective solar bands in the 0.4–1.0 µm range. It was found that for the past decade, the reflectivity change of the site is within 1.35% at 0.64 µm, and interannual variability is within 2%. The site is able to resolve calibration biases between instruments at a level of ~1%. The usefulness of the site is demonstrated by comparing observations from seven satellite instruments involving four space agencies, including OrbView-2–SeaWiFS, Terra–Aqua MODIS, Earth Observing 1 (EO-1) – Hyperion, Meteorological Operational satellite programme (MetOp) – Advanced Very High Resolution Radiometer (AVHRR), Envisat Medium Resolution Imaging Spectrometer (MERIS) – dvanced Along-Track Scanning Radiometer (AATSR), and Landsat 7 Enhanced Thematic Mapper Plus (ETM+). Dome C is a promising candidate site for climate quality calibration of satellite radiometers towards more consistent satellite measurements, as part of the framework for climate change detection and data quality assurance for the Global Earth Observation System of Systems (GEOSS).

Canadian Journal of Remote Sensing↗
USGS2023

Characterizing lung particulates using quantitative microscopy in coal miners with severe pneumoconiosis

Context.— Current approaches for characterizing retained lung dust using pathologists' qualitative assessment or scanning electron microscopy with energy-dispersive spectroscopy (SEM/EDS) have limitations. Objective.— To explore polarized light microscopy coupled with image-processing software, termed quantitative microscopy–particulate matter (QM-PM), as a tool to characterize in situ dust in lung tissue of US coal miners with progressive massive fibrosis. Design.— We developed a standardized protocol using microscopy images to characterize the in situ burden of birefringent crystalline silica/silicate particles (mineral density) and carbonaceous particles (pigment fraction). Mineral density and pigment fraction were compared with pathologists' qualitative assessments and SEM/EDS analyses. Particle features were compared between historical (born before 1930) and contemporary coal miners, who likely had different exposures following changes in mining technology. Results.— Lung tissue samples from 85 coal miners (62 historical and 23 contemporary) and 10 healthy controls were analyzed using QM-PM. Mineral density and pigment fraction measurements with QM-PM were comparable to consensus pathologists' scoring and SEM/EDS analyses. Contemporary miners had greater mineral density than historical miners (186 456 versus 63 727/mm 3 ; P = .02) and controls (4542/mm 3 ), consistent with higher amounts of silica/silicate dust. Contemporary and historical miners had similar particle sizes (median area, 1.00 versus 1.14 μm 2 ; P = .46) and birefringence under polarized light (median grayscale brightness: 80.9 versus 87.6; P = .29). Conclusions.— QM-PM reliably characterizes in situ silica/silicate and carbonaceous particles in a reproducible, automated, accessible, and time/cost/labor-efficient manner, and shows promise as a tool for understanding occupational lung pathology and targeting exposure controls.

Archives of Pathology and Laboratory Medicine↗
USGS2001

Lack of surface-associated microorganisms in a mixed species community of freshwater Unionidae

To determine whether unionids contain surface-attached endosymbiotic bacteria, ciliates, or fungi, we used scanning electron microscopy to examine the epithelial surface of various organs within the digestive systems and mantle cavity of temperate river and lake unionids on a seasonal basis. We also cultured material removed from the lumen of these same organs and from the mantle cavity to detect cellobiose-, cellulose-, and chitin- degrading microbes. No true endosymbiotic fauna were observed attached to the surface of the digestive or mantle tissues of any species of unionid. Microbial growth on cellulose or chitin bacteriological media varied by season and habitat, but not by unionid species or source of the isolate. Lake unionids did not contain microbes capable of digesting cellulose or chitin, whereas unionids from the river site did in March and August, but not in December. Since these cultured cellulose- and chitin-degrading bacteria were never found attached to any unionid tissues, they appeared to be transient forms, not true endosymbionts. Microbes capable of digesting cellobiose were found in every unionid collected in all seasons and habitats, but again, no microbes were directly observed attached to unionid tissues. If unionids, like most other vertebrates, lack digestive enzymes required to initiate primary bond breakage, then the lack of cellulolytic and chitinolytic endosymbionts would affect the ability to utilize cellulose or chitin foods. Thus, in captivity dry feeds based on corn, soybeans, or nauplii should be pre-digested to ensure maximum absorption of nutrients by unionids. The lack of celluloytic or chitinolytic endosymbionts should not affect relocation success, though the seasonal role of transient microbes in unionid nutrition requires further investigation.

Journal of Shellfish Research↗
USGS2005

Discovery of 100-160-year-old iceberg gouges and their relation to halibut habitat in Glacier Bay, Alaska

Side-scan sonar and multibeam imagery of Glacier Bay, Alaska, revealed complex iceberg gouge patterns at water depths to 135 m on the floor of Whidbey Passage and south to the bay entrance. These previously undiscovered gouges likely formed more than 100 years ago as the glacier retreated rapidly up Glacier Bay. Gouged areas free of fine sediment supported greater biodiversity of Pacific halibut Hippoglossus stenolepsis than nearby sediment-filled gouges, probably due to increased habitat complexity. Small Pacific halibut were forund more frequently in sediment-free gouged areas, presumably due to higher prey abundance. In contrast, large Pacific halibut were found more frequently on soft substrates such as sediment-filled gouges, where they could bury themselves and ambush prey.

American Fisheries Society Symposium↗
USGS1992

Surficial substrates and bathymetry of five historical lake trout spawning reefs in near-shore waters of the Great Lakes

The reestablishment of self-sustaining stocks of lake trout (Salvelinus namaycush) in the lower four Great Lakes has been substantially impeded because planted fish do not produce enough progeny that survive and reproduce. The causes for this failure are unknown, but many historical spawning sites of lake trout have been degraded by human activities and can no longer produce viable swim-up fry. In this study, we used side-scan sonar and an underwater video camera to survey, map, and evaluate the sustainability of one reef in each of the five Great Lakes for lake trout spawning and fry production. At four of the reef sites, we found good-to-excellent substrate for spawning and fry production by the shallow-water strains of lake trout that are now being planted. These substrates were in water 6-22 m deep and were composed largely of rounded or angular rubble and cobble. Interstitial spaces in these substrates were 20 cm or deeper and would protect naturally spawned eggs and fry from predators, ice scour, and buffeting by waves and currents. Subsequent studies of egg survival by other researchers confirmed our evaluation that the best substrates at two of these sites still have the potential to produce viable swim-up fry.

Technical Report↗