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At least 829 records · Page 46Linked to original sources
USGS2012

Three pathogens in sympatric populations of pumas, bobcats, and domestic cats: Implications for infections disease transmission

Anthropogenic landscape change can lead to increased opportunities for pathogen transmission between domestic and non-domestic animals. Pumas, bobcats, and domestic cats are sympatric in many areas of North America and share many of the same pathogens, some of which are zoonotic. We analyzed bobcat, puma, and feral domestic cat samples collected from targeted geographic areas. We examined exposure to three pathogens that are taxonomically diverse (bacterial, protozoal, viral), that incorporate multiple transmission strategies (vector-borne, environmental exposure/ingestion, and direct contact), and that vary in species-specificity. Bartonella spp., Feline Immunodeficiency Virus (FIV), and Toxoplasma gondii IgG were detected in all three species with mean respective prevalence as follows: puma 16%, 41% and 75%; bobcat 31%, 22% and 43%; domestic cat 45%, 10% and 1%. Bartonella spp. were highly prevalent among domestic cats in Southern California compared to other cohort groups. Feline Immunodeficiency Virus exposure was primarily associated with species and age, and was not influenced by geographic location. Pumas were more likely to be infected with FIV than bobcats, with domestic cats having the lowest infection rate. Toxoplasma gondii s eroprevalence was high in both pumas and bobcats across all sites; in contrast, few domestic cats were seropositive, despite the fact that feral, free ranging domestic cats were targeted in this study. Interestingly, a directly transmitted species-specific disease (FIV) was not associated with geographic location, while exposure to indirectly transmitted diseases – vector-borne for Bartonella spp. and ingestion of oocysts via infected prey or environmental exposure for T. gondii – varied significantly by site. Pathogens transmitted by direct contact may be more dependent upon individual behaviors and intra-specific encounters. Future studies will integrate host density, as well as landscape features, to better understand the mechanisms driving disease exposure and to predict zones of cross-species pathogen transmission among wild and domestic felids.

PLoS ONE↗
USGS2015

Avian influenza ecology in North Atlantic sea ducks: Not all ducks are created equal

Wild waterfowl are primary reservoirs of avian influenza viruses (AIV). However the role of sea ducks in the ecology of avian influenza, and how that role differs from freshwater ducks, has not been examined. We obtained and analyzed sera from North Atlantic sea ducks and determined the seroprevalence in those populations. We also tested swab samples from North Atlantic sea ducks for the presence of AIV. We found relatively high serological prevalence (61%) in these sea duck populations but low virus prevalence (0.3%). Using these data we estimated that an antibody half-life of 141 weeks (3.2 years) would be required to attain these prevalences. These findings are much different than what is known in freshwater waterfowl and have implications for surveillance efforts, AIV in marine environments, and the roles of sea ducks and other long-lived waterfowl in avian influenza ecology.

PLoS ONE↗
USGS1965

Infectious pancreatic necrosis: its detection and identification

Ultimate control of infectious pancreatic necrosis (IPN) in hatcheries depends largely upon learning where the virus occurs. To detect the presence of virus either susceptible fish or susceptible fish cell cultures may be used as test systems. In modern virology, it is generally agreed that cell cultures are more convenient, are usually a much more sensitive test system, and allow more rapid determinations.

Progressive Fish-Culturist↗
USGS1947

Mortality of smelt, Osmerus mordax (Mitchill), in Lakes Huron and Michigan during the fall and winter of 1942-1943

The mortality that nearly exterminated the huge stocks of smelt in Lakes Huron and Michigan during the fall and winter of 1942–1943 appears to have originated in central Lake Huron in the Saginaw Bay area in late September or early October 1942. The mortality spread rapidly northward reaching the Drummond Island area about the latter part of October and the St. Ignace region of the Straits of Mackinac near the end of the month. In the latter part of October smelt died also in the Canadian waters of Lake Huron including North Channel and Georgian Bay but exact details as to time and course are lacking. There is some evidence that the epidemic had not reached the Ontario shore of central Lake Huron by late May 1943. Spreading through northern Lake Michigan the mortality had penetrated as far south as Grand Traverse Bay by November 19 and as far west as Point Aux Barques, Michigan, by November 26, 1942. Smelt were reported to be dying in Lake Charlevoix, Michigan, in early February 1943, and in Green Bay toward the middle of that month. The mortality did not reach Crystal Lake where in contrast to Lake Charlevoix a dam barred the passage of fish from Lake Michigan. At the time of the 1943 spring spawning run (April) only a few scattered survivors remained from the vast populations. After consideration of possible causes, it was concluded that the mortality could be explained only as resulting from a communicable disease (bacteria or virus). This explanation alone is in harmony with the following facts: the mortality was progressive, spreading from one area into adjacent areas over a period of at least 4 1/2 months and under a great diversity of habitat conditions; only smelt were affected but within the species death overtook fish of all sizes from 2 inches on and all ages of both sexes, mature and immature; the mortality penetrated Lake Charlevoix where the passage of fish to and from Lake Michigan was possible but did not reach Crystal Lake where the passage from Lake Michigan was barred by a dam; the epidemic did not reach other inland lakes where free and easy access from the Great Lakes was impossible nor did it extend to Lakes Superior, Erie, and Ontario. Considered at first as a nuisance and a threat to the native fishes of the Great Lakes, the smelt ultimately became a fish of primary importance to commercial fishermen, sportsmen, and others. In Green Bay, the center of the commercial fishery, smelt became the dominant commercial species, yielding more than 4 million pounds in some years. Almost all of the commercial production was from nets set under the ice. The take by amateurs and others who dipped smelt from streams during the spawning run was even greater, amounting to as much as 5 1/2 million pounds in a single year in the State of Michigan alone (the yield in Wisconsin may have been nearly as great). The mortality of smelt was a severe blow to the nation's war-time food-production program. It is estimated that in 1943, in which year elaborate preparations had been made for the efficient utilization of the spawning-run production, the mortality reduced the output of smelt by about 13 million pounds. The total loss through the present (1946) season can be set in the neighborhood of 50 million pounds. The first indication of a recovery of the smelt came in 1945 when a small amount was produced commercially in Green Bay and numerous light runs occurred in streams tributary to Lakes Huron and Michigan. The general level of abundance in 1945, however, is believed to have been less than 10 per cent of that of “pre-mortality” years. Such information as is available for 1946 suggests considerable further improvement in this year. Given good survival of young, it is anticipated that a large rise in the abundance of smelt can occur in 1947 and that by 1948 or 1949 the size of the populations should no longer be influenced by the number of spawners available in preceding years. The smelt from the Escanaba area of Green Bay were without exception significantly longer and heavier in 1944 and 1945 than were fish of corresponding age captured in the same region in 1941. Furthermore, three of four comparisons indicated significantly greater size in 1945 than in 1944. This improvement in growth rate is believed to have been associated with the reduction in the smelt population brought about by the 1942–1943 mortality.

Transactions of the American Fisheries Society↗
USGS2010

Susceptibility of three stocks of pacific herring to viral hemorrhagic septicemia

Laboratory challenges using specific-pathogen-free Pacific herring Clupea pallasii from three distinct populations indicated that stock origin had no effect on susceptibility to viral hemorrhagic septicemia (VHS). All of the populations were highly susceptible to the disease upon initial exposure, with significantly greater cumulative mortalities occurring in the exposed treatment groups (56.3-64.3%) than in the unexposed control groups (0.8-9.0%). Interstock differences in cumulative mortality were not significant. The virus loads in the tissues of fish experiencing mortality were 10-10,000 times higher during the acute phase of the epizootics (day 13 postexposure) than during the recovery phase (days 30-42). Survivors of the epizootics were refractory to subsequent VHS, with reexposure of VHS survivors resulting in significantly less cumulative mortality (1.2-4.0%) than among positive controls (38.1-64.4%); interstock differences in susceptibility did not occur after reexposure. These results indicate that data from experiments designed to understand the ecology of VHS virus in a given stock of Pacific herring are broadly applicable to stocks throughout the northeastern Pacific.

Journal of Aquatic Animal Health↗
USGS2010

Migration of waterfowl in the east asian flyway and spatial relationship to HPAI H5N1 outbreaks

Poyang Lake is situated within the East Asian Flyway, a migratory corridor for waterfowl that also encompasses Guangdong Province, China, the epicenter of highly pathogenic avian influenza (HPAI) H5N1. The lake is the largest freshwater body in China and a significant congregation site for waterfowl; however, surrounding rice fields and poultry grazing have created an overlap with wild waterbirds, a situation conducive to avian influenza transmission. Reports of HPAI H5N1 in healthy wild ducks at Poyang Lake have raised concerns about the potential of resilient free-ranging birds to disseminate the virus. Yet the role wild ducks play in connecting regions of HPAI H5N1 outbreak in Asia is hindered by a lack of information about their migratory ecology. During 2007-08 we marked wild ducks at Poyang Lake with satellite transmitters to examine the location and timing of spring migration and identify any spatiotemporal relationship with HPAI H5N1 outbreaks. Species included the Eurasian wigeon ( Anas penelope ), northern pintail ( Anas acuta ), common teal ( Anas crecca ), falcated teal ( Anas falcata ), Baikal teal ( Anas formosa ), mallard ( Anas platyrhynchos ), garganey ( Anas querquedula ), and Chinese spotbill ( Anas poecilohyncha ). These wild ducks (excluding the resident mallard and Chinese spotbill ducks) followed the East Asian Flyway along the coast to breeding areas in northern China, eastern Mongolia, and eastern Russia. None migrated west toward Qinghai Lake (site of the largest wild bird epizootic), thus failing to demonstrate any migratory connection to the Central Asian Flyway. A newly developed Brownian bridge spatial analysis indicated that HPAI H5N1 outbreaks reported in the flyway were related to latitude and poultry density but not to the core migration corridor or to wetland habitats. Also, we found a temporal mismatch between timing of outbreaks and wild duck movements. These analyses depend on complete or representative reporting of outbreaks, but by documenting movements of wild waterfowl, we present ecological knowledge that better informs epidemiological investigations seeking to explain and predict the spread of avian influenza viruses. ?? 2010 American Association of Avian Pathologists.

Avian Diseases↗
USGS2012

Reversion to virulence and efficacy of an attenuated canarypox vaccine in Hawai'i 'Amakihi ( Hemignathus Virens )

Vaccines may be effective tools for protecting small populations of highly susceptible endangered, captive-reared, or translocated Hawaiian honeycreepers from introduced Avipoxvirus , but their efficacy has not been evaluated. An attenuated Canarypox vaccine that is genetically similar to one of two passerine Avipoxvirus isolates from Hawai‘i and distinct from Fowlpox was tested to evaluate whether Hawai‘i ‘Amakihi ( Hemignathus virens ) can be protected from wild isolates of Avipoxvirus from the Hawaiian Islands. Thirty-one (31) Hawai‘i ‘Amakihi were collected from high-elevation habitats on Mauna Kea Volcano, where pox transmission is rare, and randomly divided into two groups. One group was vaccinated with Poximune C®, whereas the other group received a sham vaccination with sterile water. Four of 15 (27%) vaccinated birds developed life-threatening disseminated lesions or lesions of unusually long duration, whereas one bird never developed a vaccine-associated lesion or “take.” After vaccine lesions healed, vaccinated birds were randomly divided into three groups of five and challenged with either a wild isolate of Fowlpox (FP) from Hawai‘i, a Hawai‘i ‘Amakihi isolate of a Canarypox-like virus (PV1), or a Hawai‘i ‘Amakihi isolate of a related, but distinct, passerine Avipoxvirus (PV2). Similarly, three random groups of five unvaccinated ‘Amakihi were challenged with the same virus isolates. Vaccinated and unvaccinated ‘Amakihi challenged with FP had transient infections with no clinical signs of infection. Mortality in vaccinated ‘Amakihi challenged with PV1 and PV2 ranged from 0% (0/5) for PV1 to 60% (3/5) for PV2. Mortality in unvaccinated ‘Amakihi ranged from 40% (2/5) for PV1 to 100% (5/5) for PV2. Although the vaccine provided some protection against PV1, both potential for vaccine reversion and low efficacy against PV2 preclude its use in captive or wild honeycreepers.

Hawaii↗
USGS2015

Pathogen exposure varies widely among sympatric populations of wild and domestic felids across the United States

Understanding how landscape, host, and pathogen traits contribute to disease exposure requires systematic evaluations of pathogens within and among host species and geographic regions. The relative importance of these attributes is critical for management of wildlife and mitigating domestic animal and human disease, particularly given rapid ecological changes, such as urbanization. We screened >1,000 samples from sympatric populations of puma (Puma concolor), bobcat (Lynx rufus) and domestic cat (Felis catus) across urban gradients in six sites, representing three regions, in North America for exposure to a representative suite of bacterial, protozoal and viral pathogens (Bartonella sp., Toxoplasma gondii, feline herpesvirus-1, feline panleukopenea virus, feline calicivirus, feline immunodeficiency virus). We evaluated prevalence within each species, and examined host trait and land cover determinants of exposure-providing an unprecedented analysis of factors relating to potential for infections in domesticated and wild felids. Prevalence differed among host species (highest for puma and lowest for domestic cat) and was greater for indirectly transmitted pathogens. Sex was inconsistently predictive of exposure to directly transmitted pathogens only, and age infrequently predictive of both direct and indirectly transmitted pathogens. Determinants of pathogen exposure were widely divergent between the wild felid species. For puma, suburban landuse predicted increased exposure to Bartonella sp. in southern California, and FHV-1 exposure increased near urban edges in Florida. This may suggest inter-specific transmission with domestic cats via flea vectors (California) and direct contact (Florida) around urban boundaries. Bobcats captured near urban areas had increased exposure to T. gondii in Florida, suggesting an urban source of prey. Bobcats captured near urban areas in Colorado and Florida had higher FIV exposure, possibly suggesting increased intra-specific interactions through pile-up of home ranges. Beyond these regional and pathogen specific relationships, proximity to the wildland urban interface did not generally increase the probability of disease exposure in wild or domestic felids, emphasizing the importance of local ecological determinants. Indeed, pathogen exposure was often negatively associated with the wildland urban interface for all felids. Our analyses suggest cross-species pathogen transmission events around this interface may be infrequent, but followed by self-sustaining propagation within the new host species.

California, Colorado,Florida↗
USGS2008

Elk calf survival and mortality following wolf restoration to Yellowstone National Park

We conducted a 3‐year study (May 2003–Apr 2006) of mortality of northern Yellowstone elk ( Cervus elaphus ) calves to determine the cause for the recruitment decline (i.e., 33 calves to 13 calves/100 adult F) following the restoration of wolves ( Canis lupus ). We captured, fit with radiotransmitters, and evaluated blood characteristics and disease antibody seroprevalence in 151 calves ≤6 days old (68M:83F). Concentrations (x̄, SE) of potential condition indicators were as follows: thyroxine (T4; 13.8 μg/dL, 0.43), serum urea nitrogen (SUN; 17.4 mg/dL, 0.57), γ‐glutamyltransferase (GGT; 66.4 IU/L, 4.36), gamma globulins (GG; 1.5 g/dL, 0.07), and insulin‐like growth factor‐1 (IGF‐1; 253.6 ng/mL, 9.59). Seroprevalences were as follows: brucellosis ( Brucella abortus ; 3%), bovine‐respiratory syncytial virus (3%), bovine‐viral‐diarrhea virus type 1 (25%), infectious‐bovine rhinotracheitis (58%), and bovine parainfluenza‐3 (32%). Serum urea nitrogen, GGT, GG, and IGF‐1 varied with year; T4, SUN, and GG varied with age ( P ≤ 0.01); and SUN varied by capture area ( P = 0.02). Annual survival was 0.22 (SE = 0.035, n = 149) and varied by calving area but not year. Neonates captured in the Stephens Creek/Mammoth area of Yellowstone National Park, USA, had annual survival rates >3× higher (0.54) than those captured in the Lamar Valley area (0.17), likely due to the higher predator density in Lamar Valley. Summer survival (20 weeks after radiotagging) was 0.29 (SE = 0.05, n = 116), and calving area, absolute deviation from median birth date, and GG were important predictors of summer survival. Survival during winter (Nov‐Apr) was 0.90 (SE= 0.05, n = 42), and it did not vary by calving area or year. Sixty‐nine percent ( n = 104) of calves died within the first year of life, 24% ( n = 36) survived their first year, and 7% ( n = 11) had unknown fates. Grizzly bears ( Ursus arctos ) and black bears ( Ursus americanus ) accounted for 58–60% ( n = 60–62) of deaths, and wolves accounted for 14–17% ( n = 15–18). Summer predation (95% of summer deaths) increased, and winter malnutrition (0% of winter deaths) decreased, compared with a similar study during 1987–1990 (72% and 58%, respectively). Physiological factors (e.g., low levels of GG) may predispose calves to predation. Also, the increase in bear numbers since wolf restoration and spatial components finer than the northern range should be considered when trying to determine the causes of the northern Yellowstone elk decline. This is the first study to document the predation impacts from reintroduced wolves on elk calf mortality in an ecosystem already containing established populations of 4 other major predators (i.e., grizzly and black bears, cougars [ Puma concolor ], and coyotes [ Canis latrans ]). The results are relevant to resource managers of the Yellowstone ecosystem in understanding the dynamics of the elk population, in providing harvest quota recommendations for local elk hunts to the Montana Department of Fish, Wildlife and Parks, the United States Fish and Wildlife Service regarding wolf and grizzly bear recovery, and to all areas worldwide where predators are increasing, by providing managers with information about potential carnivore impacts on elk populations.

Idaho, Montana, Wyoming↗
USGS1991

Isolation of poxvirus from debilitating cutaneous lesions on four immature grackles (Quiscalus sp.)

Poxvirus was isolated from nodules on four immature grackles ( Quiscalus sp.) collected in two residential areas of Victoria, Texas. All of the birds were emaciated and had nodules on the eyelids, bill, legs, toes, and areas of the skin on the wings, neck, and ventral abdomen. These pox nodules were extensive and probably interfered with both sight and flight. The preliminary diagnosis was confirmed by virus isolation, histopathology, and electron microscopy. Poxvirus was isolated on the chorioallantoic membrane of embryonated hen's eggs and in Muscovy duck embryo fibroblast cell culture. Phaenicia calliphoridae (blowfly) larvae were found in one of the pox nodules, raising the possibility of mechanical transmission of the virus by contaminated adult blowfiles.

Texas↗
USGS1993

Duck viral enteritis in domestic muscovy ducks in Pennsylvania

Duck viral enteritis (DVE) outbreaks occurred at two different locations in Pennsylvania in 1991 and 1992. In the first outbreak, four ducks died out of a group of 30 domestic ducks; in the second outbreak, 65 ducks died out of a group of 114 domestic ducks, and 15 domestic geese died as well. A variety of species of ducks were present on both premises, but only muscovy ducks (Cairina moschata) died from the disease. On necropsy, gross lesions included hepatomegaly with petechial hemorrhages, petechial hemorrhages in the abdominal fat, petechial hemorrhages on the epicardial surface of the heart, and multifocal to coalescing areas of fibrinonecrotic material over the mucosal surface of the trachea, esophagus, intestine, and cloaca. Histologically, the liver had random multifocal areas of necrosis and eosinophilic intranuclear inclusion bodies in hepatocytes. DVE virus was isolated and identified using muscovy duck embryo fibroblast inoculation and virus neutralization.

Pennsylvania↗
USGS1987

Microbial infections in a declining wild turkey population in Texas

A survey was conducted at 5 locations in Texas for avian pathogens that might adversely affect wild turkey ( Meleagris gallopavo ) productivity and survival. At 1 site, the Rob and Bessie Welder Wildlife Refuge (WWR), turkeys have declined precipitously in recent years. During the winters of 1983-85, 442 wild turkeys were caught with cannon and drop nets, 161 of these on WWR. Blood samples were drawn for serologic evaluation, and cloacal and tracheal swabs were collected for isolation attempts. Salmonella spp. bacteria, Newcastle disease virus (NDV), and avian influenza virus (AIV) were not detected in any samples tested. Serologic tests for antibodies to NDV and AIV also were negative. Many mycoplasma isolates were recovered from turkeys from every location. Characterization of these isolates indicated that several species were present. None were species typically associated with mycoplasmosis in domestic turkeys, such as Mycoplasma gallisepticum (MG), M. meleagridis (MM), or M. synoviae (MS), although antibodies to these pathogens were detected in turkeys at every location sampled. There was no evidence to link any of these disease causing agents to the decline observed in the population of wild turkeys on the WWR.

Texas↗
USGS2012

AquaPathogen X--A template database for tracking field isolates of aquatic pathogens

AquaPathogen X is a template database for recording information on individual isolates of aquatic pathogens and is available for download from the U.S. Geological Survey (USGS) Western Fisheries Research Center (WFRC) website (http://wfrc.usgs.gov). This template database can accommodate the nucleotide sequence data generated in molecular epidemiological studies along with the myriad of abiotic and biotic traits associated with isolates of various pathogens (for example, viruses, parasites, or bacteria) from multiple aquatic animal host species (for example, fish, shellfish, or shrimp). The simultaneous cataloging of isolates from different aquatic pathogens is a unique feature to the AquaPathogen X database, which can be used in surveillance of emerging aquatic animal diseases and clarification of main risk factors associated with pathogen incursions into new water systems. As a template database, the data fields are empty upon download and can be modified to user specifications. For example, an application of the template database that stores the epidemiological profiles of fish virus isolates, called Fish ViroTrak (fig. 1), was also developed (Emmenegger and others, 2011).

Fact Sheet↗
USGS2016

Science to support aquatic animal health

Healthy aquatic ecosystems are home to a diversity of plants, invertebrates, fish and wildlife. Aquatic animal populations face unprecedented threats to their health and survival from climate change, water shortages, habitat alteration, invasive species and environmental contaminants. These environmental stressors can directly impact the prevalence and severity of disease in aquatic populations. For example, periodic fish kills in the upper Chesapeake Bay Watershed are associated with many different opportunistic pathogens that proliferate in stressed fish populations. An estimated 80 percent of endangered juvenile Puget Sound steelhead trout die within two weeks of entering the marine environment, and a role for disease in these losses is being investigated. The introduction of viral hemorrhagic septicemia virus (VHSV) into the Great Lakes—a fishery worth an estimated 7 billion dollars annually—resulted in widespread fish die-offs and virus detections in 28 different fish species. Millions of dying sea stars along the west coast of North America have led to investigations into sea star wasting disease. U.S. Geological Survey (USGS) scientists are assisting managers with these issues through ecological investigations of aquatic animal diseases, field surveillance, and research to promote the development of mitigation strategies.

Fact Sheet↗
USGS2010

Microbial and geochemical investigations of dissolved organic carbon and microbial ecology of native waters from the Biscayne and Upper Floridan Aquifers

Groundwater resources in the United States are under ever-increasing demands for potable, irrigation, and recreational uses. Additionally, aquifer systems are being used or targeted for use as storage areas for treated surface waters and (or) groundwaters via injection (for example, aquifer storage and recovery). To date, the influence that the nutrients, including carbon, in the injected water have on native microbial communities and the biogeochemistry in the subsurface zones used for storage of the injectate has not been determined. In this report, we describe a series of experiments that establishes a baseline dataset for the quantity and quality of organic and inorganic carbon and nutrients in the Biscayne Aquifer (BA) and Upper Floridan Aquifer (UFA) in south Florida. The most significant differences between the BA (26 meters below surface) and UFA (366 meters below surface) are the average specific conductance (0.552 and 6.12 microsiemens per centimeter, respectively), dissolved oxygen (1.6 and 0 milligrams per liter, respectively), and oxidation-reduction potential (40.3 and -358 millivolts, respectively). The dissolved organic carbon from the BA is characterized by carbon originating from terrestrial sources and microbial activities, while the UFA has a distinctive microbial signature. Acetate and lactate are the dominant carbon constituents in both aquifers. Additionally, components of the dissolved organic carbon from the UFA have a total trihalomethane-formation potential that is approximately threefold greater than the maximum contaminat level of 80 micrograms per liter established by the U.S. Environmental Protection Agency. The average native bacterial abundances in the aquifers are similar with 4.69x10^4 cells per milliliter in the BA and 1.33x10^4 cells per milliliter in the UFA. The average bacteriophage abundances are also similar with 1.15x10^5 virus-like particles in the BA and 1.92x10^5 virus-like particles in the UFA. Interestingly, ciliated protozoa are present in both aquifers. The average abundance of ciliates in the BA (2.97x10^3 ciliates per milliliter) is approximately twentyfold greater than abundances in the UFA (1.39x10^2 ciliates per milliliter). Collectively, these data indicate that microbial processes are the dominant contributor to the cycling of carbon and inorganic carbon in the BA and may be the only carbon cycling process in the UFA, as this aquifer has not had a terrestrial influx of carbon for more than 15,000 years. The rates of carbon, in the form of acetate, utilization by the native microbial communities are significantly different between the two aquifers. Based on data from 14C-acetate-utilization experiments, the microbial communities in the BA turn over the native acetate in 2.5 years, whereas communities in the UFA turn over native acetate in 6.8 years. These data support the hypothesis derived from the microbial-abundance data, in that the carbon for bacterial maintainence and growth is recycled from bacterial biomass released during cell lysis, especially in the UFA. An in situ diffusion chamber was designed to retain bacterial cells within the chamber while allowing native water constituents to move through the chamber. A series of 1-week deployments of chambers filled with fluorescent beads, inactivated native bacteria and laboratory grown and viable bacteria into the UFA, permitted by the State of Florida Environmental Protection Agency, was successfully completed. This was the first time this type of deployment into an aquifer system that is used for potable water supply has been permitted within the United States. This technology will allow, for the first time, in situ studies on the survival of microbial indicators of fecal pollution and true pathogens in groundwater systems.

Florida↗
USGS2026

Evaluation of pathogen risks and testing considerations for Chinook salmon egg movements between New Zealand and California

Executive Summary Oncorhynchus tshawytscha (Walbaum in Artedi, 1792; Chinook salmon) were historically abundant in the McCloud River but are now extirpated from this tributary owing to dam construction and lack of passage. Planning efforts to restore populations above Shasta and Keswick Dams are currently underway, including an evaluation of potential source populations. One potential source is New Zealand Chinook salmon, which are believed to have originated from tributaries of the Sacramento River. These fish could be returned to California if reintroduction risks, including risks of pathogen introduction, could be sufficiently mitigated. The U.S. Geological Survey was contracted to provide scientific support for reintroduction efforts, including evaluating the risks of pathogen transmission via the movement of Chinook salmon eggs from New Zealand to the McCloud River. This report estimates pathogen risks associated with egg movement and considers epidemiological and biosecurity measures to minimize these risks. Pathogen risks associated with the movement of Chinook salmon eggs from New Zealand were evaluated based on pathogen virulence, transmission route, and geographic distribution. These criteria identified 14 moderate- and high-risk pathogens out of the 30 pathogens evaluated. Pathogen species and strains were considered high risk if they have the potential for vertical transmission (that is, transmission from parent to offspring), are moderately or highly virulent, and are exotic to the Sacramento River Basin. According to these criteria, we identified the following pathogens as high risk: New Zealand rickettsia-like organisms 1 and 2. —These bacterial pathogens have been associated with mortality events in farmed Chinook salmon from the South Island of New Zealand but have not been detected in other regions. Pilchard orthomyxovirus (POMV). —POMV has been detected in Sardina pilchardus (Walbaum, 1792; pilchards) and Salmo salar (Linnaeus, 1758; Atlantic salmon) from the coasts of southern Australia and Tasmania. POMV can cause relatively high mortality rates and may be indirectly transmitted via contaminated water sources. Infectious pancreatic necrosis virus (IPNV). —IPNV has a wide geographic distribution and is present in the Sacramento River Basin, but the IPNV-like viruses detected in Australia and New Zealand are unique from those found in the United States. Yersinia ruckeri . —This bacterial pathogen is the causative agent of enteric redmouth disease and has a widespread geographic distribution. However, the strains that are present in Australia and New Zealand are unique from those found in North America. Strategic use of testing and biosecurity measures can minimize pathogen risks associated with the movement of eggs. The most effective measures include iodophor treatment of eggs to remove external pathogens, testing of all the adult fish from which gametes are obtained, and a quarantine period after transport to confirm pathogen testing results. Additional measures to enhance biosecurity could include testing the quarantined fish following emergence and (or) developing a fish health history of the source population through pathogen monitoring.

California↗
USGS2006

Microbial Quality of the Nation's Ground-Water Resources, 1993-2004

As part of the National Water-Quality Assessment (NAWQA) program, microbiological data were collected from wells in 22 NAWQA study units during 1993-2004. The wells constituted the sampling networks for three major NAWQA efforts--the major aquifer study, the land-use study, and source-water quality assessments of ground water used for public supplies. Sixteen principal aquifers were represented by these well networks. Samples of untreated ground water were analyzed for concentrations of fecal-indicator bacteria, which included the total-coliform bacteria, fecal-coliform bacteria, and Escherichia coli, and for the presence of somatic and male-specific coliphage viruses. Analyses of the samples showed that coliform bacteria occur relatively frequently-nearly 30 percent of all wells tested positive-and that domestic wells commonly are contaminated by total coliform bacteria, with 33 percent of these wells testing positive. Coliphage viruses were present in 10 percent or fewer of the wells sampled in the Central Columbia Plateau-Yakima, Georgia-Florida, San Joaquin, and Trinity study units, which represent the Columbia Plateau, Floridan, Central Valley, and Coastal Lowlands principal aquifers, respectively. The frequency of detections and concentrations of total coliform bacteria generally were higher in samples from domestic wells than in samples from public-supply wells; in fractured or porous rock materials (carbonate rocks) than in unconsolidated materials (mixtures of sand, gravel, clay); and in principal aquifers with median depths of sampled wells ranging from 100 to 200 feet than in principal aquifers with median depths of sampled wells less than 100 feet or greater than 200 feet. The waters most affected by the presence of coliform bacteria were those in the Valley and Ridge, the Floridan, and the Piedmont and Blue Ridge aquifers, where more than 50 percent of the study wells tested positive for these bacteria. The numbers of wells with detections of coliform bacteria were significantly lower for the Glacial Deposits, Stream and River Valley, Columbia Plateau, Basin and Range, High Plains, Southeastern Coastal Plain, and Coastal Lowlands aquifers. Of the 16 principal aquifers sampled, wells in the Valley and Ridge had the highest overall concentrations of total coliforms, with a median of 2 colony-forming units per 100 milliliters. Elevated concentrations of coliform bacteria (greater than 300 colony-forming units per 100 milliliters) also were reported for wells completed in the Mississippian-Pennsylvanian aquifer and the Ordovician aquifer in lower Tennessee. More than 50 percent of wells completed in carbonate or crystalline rocks tested positive for coliform bacteria. The Floridan, Piedmont and Blue Ridge, Ordovician (lower Tennessee), and Valley and Ridge aquifers are composed of these types of rocks. The lowest detection frequencies (less than 5 percent) were for wells in the Basin and Range and the Snake River aquifers. The materials that constitute these aquifers primarily are unconsolidated sand, gravel, and clay, or basalt with interbeds of sand, gravel, or clay. No strong correlations between concentrations of total coliforms and well depths were identified, nor were there any correlations between total coliform concentrations and selected chemical constituents in the waters. Other factors, such as geohydrologic characteristics, proximity of contaminating sources, interactions with surface water, or well-construction features (including the age of the well), likely control the presence and transport of coliform bacteria in the ground water.

Scientific Investigations Report↗
USGS2011

Effects of experimental passive artificial recharge of treated surface water on water quality in the Equus Beds Aquifer, 2009-2010

Declining water levels and concerns about the migration of a known saltwater plume upgradient from public supply wells prompted the City of Wichita to investigate the feasibility of using artificial recharge to replenish the water supply in the Equus Beds aquifer. After preliminary testing, the City of Wichita began Phase I of the Equus Beds Aquifer Storage and Recovery Project in 2006. In 2009, the City of Wichita installed an experimental passive gravity recharge well and trench system to increase artificial recharge at Recharge Basin 1, one of the six Phase ? recharge sites. The U.S. Geological Survey collected water samples from 13 sites and maintained 8 continuous monitors to test the recharge capacity of the experimental passive recharge system, the effect of the recharge on geochemistry of the aquifer, and the fate of bacteria and viruses present in the recharge water. About 576,000 gallons of treated surface water from the Little Arkansas River were recharged through the passive recharge well and trench system into the Equus Beds aquifer during April 2009. In May 2009, U.S. Geological Survey tests detected that bacterial and viral indicators (total coliform, fecal coliform, Escherichia coli, coliphage virus, and Clostridium perfringens) were entering the Recharge Basin 1 wells through the recharge system and recharge was discontinued. The City of Wichita disconnected the trench collection system from the passive gravity recharge well in July 2009, and in July and August 2009 withdrew 1,825,000 gallons of water from the aquifer at Recharge Basin 1 to remove the recharged water and avoid contamination of the aquifer. The original recharge rate in Recharge Basin 1 was about 10.8 gallons per day per square foot. After installation of the passive recharge system, recharge water entered the aquifer through the passive well at a rate of about 19.2 gallons per day per square foot, a per unit area increase of about 78 percent. During artificial recharge, continuous monitors recorded rising water-level altitudes in the passive gravity recharge well and nearby monitoring wells as water flowed at about 10 feet per day from the passive recharge well toward nearby downgradient monitoring wells. The increase in water level in this area would have the effect of temporarily slowing the eastward migration of saltwater from the nearby Burrton plume. Bacterial and viral indicators were detected in water samples from Recharge Basin 1 sites before and immediately after the installation of the passive gravity recharge well and trench system, during artificial recharge, and after artificial recharge. After water withdrawal in August 2009 and through the end of data collection in March 2010, detections of bacterial and viral indicators in groundwater decreased to densities similar to those before installation of the passive recharge system. Concentrations of chloride in samples collected from the trench, passive gravity recharge well, and nearby monitoring wells increased from an average of 34 milligrams per liter before artificial recharge to an average of 64 milligrams per liter during artificial recharge, reflecting the addition of recharge water with measured chloride concentrations of 62 to 94 milligrams per liter. When water was being pumped out of the aquifer through the passive gravity recharge well, chloride concentrations increased to 94 milligrams per liter in the removed water and increased to 150 milligrams per liter in the deep monitoring well nearest the passive gravity recharge well, indicating that, as water was being pumped from the passive well, water with a large chloride concentration from elsewhere in the aquifer was flowing toward the passive well. Chloride concentrations did not exceed the U.S. Environmental Protection Agency Secondary Drinking Water Regulation of 250 milligrams per liter in any Recharge Basin 1 samples collected as part of the study. Iron concentrations exceeded the U.S. Environmental Protection Agency

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