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1.13 – Emerging contaminants

Since the Industrial Revolution, a diversity of large-scale chemical innovations has impacted aquatic systems in urban environments. Beginning in the 1990s, there has been a growing scientific interest and public awareness of the effects of the chemicals used in domestic, commercial, industrial, and agricultural applications, referred to in this article as ‘emerging contaminants’ (ECs), on ecosystem and human health. The growing global population and its increasing demands on water supplies in conjunction with climate-induced changes in hydrologic regimes place stress on freshwater resources, resulting in a greater reliance on reuse of reclaimed municipal wastewater treatment plant (WWTP) effluents to meet human and environmental needs. WWTP effluents are a major source of ECs, and it is important to have an understanding of the chemical composition of the reclaimed water, because many ECs are biologically active and the effects of chronic exposure to low concentration complex mixtures are unknown. Several classes of ECs that have been shown to be widespread in the aquatic environment are discussed in this chapter, including surfactants, complexing agents, fragrances, antimicrobials, industrial chemicals, pharmaceuticals, natural and synthetic estrogens, and disinfection byproducts. All of these compounds are biologically active via a variety of modes of action, and can occur in aquatic systems at concentrations ranging from <0.001 to >100 μg l −1 .

Book chapter↗

The Value of Data – The Qatar Geologic Mapping Project

The State of Qatar is in a period of rapid development, modernization, and population growth. One of the most important factors influencing the long-term success and sustainability of future development is a comprehensive understanding of the region’s geologic regime, geotechnical conditions, natural resources, and environmental constraints. To obtain this understanding, the Ministry of Municipality and Environment (MME) of the State of Qatar has undertaken the Qatar Geological Mapping Project (QGMP). The project was envisioned with the strategic foresight to compile and utilize existing and legacy subsurface data collected as part of its massive infrastructure and development projects as the foundation for developing modern scientific resources including geologic maps, digital thematic maps, and a 3-dimensional geological model of the Doha metropolitan area. Recently, the MME, in consultation with Gannett Fleming, Inc. (GF) and the United States Geological Survey (USGS) concluded the data collection and analysis phase (Phase I) of the two-phase QGMP. Phase I included: the development of a comprehensive geotechnical relational database populated with data digitized from more than 13,000 subsurface data logs; a detailed data quality analysis and distribution assessment; an extensive gap analysis and needs assessment; and careful design of the geologic mapping and subsurface investigation programs for the next phase of the project.

Conference Paper↗

Effect of passive acoustic sampling methodology on detecting bats after declines from white nose syndrome

Concomitant with the emergence and spread of white-nose syndrome (WNS) and precipitous decline of many bat species in North America, natural resource managers need modified and/or new techniques for bat inventory and monitoring that provide robust occupancy estimates. We used Anabat acoustic detectors to determine the most efficient passive acoustic sampling design for optimizing detection probabilities of multiple bat species in a WNS-impacted environment in New York, USA. Our sampling protocol included: six acoustic stations deployed for the entire duration of monitoring as well as a 4 x 4 grid and five transects of 5-10 acoustic units that were deployed for 6-8 night sample durations surveyed during the summers of 2011-2012. We used Program PRESENCE to determine detection probability and site occupancy estimates. Overall, the grid produced the highest detection probabilities for most species because it contained the most detectors and intercepted the greatest spatial area. However, big brown bats ( Eptesicus fuscus ) and species not impacted by WNS were detected easily regardless of sampling array. Endangered Indiana ( Myotis sodalis ) and little brown ( Myotis lucifugus ) and tri-colored bats ( Perimyotis subflavus ) showed declines in detection probabilities over our study, potentially indicative of continued WNS-associated declines. Identification of species presence through efficient methodologies is vital for future conservation efforts as bat populations decline further due to WNS and other factors.

Journal of Ecology and the Natural Environment↗

Resource availability and heterogeneity affect space use and resource selection of a feral ungulate

Animals adjust their habitat use patterns in response to changes in their physiological needs and environmental conditions. Understanding the mechanisms underlying resource selection and space use across time and space reveals effects of the environment on animals' decisions. We explored the effects of habitat availability and heterogeneity on the seasonal and annual space use and resource selection of two free-roaming feral burro ( Equus asinus ) populations in the United States within distinct climate and habitat conditions: the Sonoran Desert and the Colorado Plateau. As an introduced yet protected species in the United States, understanding burros' interactions with habitat elements is important for their conservation and management, as well as the conservation of sympatric wildlife. We used GPS locations of female burros (72 animals across both study areas) to delineate annual and seasonal ranges and resource selection patterns. We evaluated effects of mean and CV of habitat covariates, including forage, distance to water, and topography, representing availability and heterogeneity of resources, on seasonal and annual range size of burros. Moreover, we explored how burro seasonal and annual resource selection patterns were affected by availability and heterogeneity of resources. In the Sonoran Desert study area, burros had smaller seasonal and annual ranges and constant resource selection patterns across a year, likely due to a freshwater lake in the area, making water a nonlimiting resource. Human presence was the greatest factor affecting range size and resource selection in the Sonoran Desert, where burros selected for areas near roads and human recreation. In the Colorado Plateau study area, where resources were more seasonal, we found larger range sizes and fluctuating resource selection patterns compared to the Sonoran Desert population. Spatial variation in forage, water, and topography significantly affected range size of burros inhabiting the Colorado Plateau study area. Productive habitats with available water support smaller ranges and a more consistent pattern of resource selection. Our results highlight the positive effect of habitat heterogeneity and the negative effect of habitat productivity on range size of animals. Our findings contribute to an improved understanding of habitat requirements for free-roaming burros that currently live under various climate and habitat conditions globally.

Arizona, Utah↗

A landscape perspective of the stream corridor invasion and habitat characteristics of an exotic (Dioscorea oppositifolia) in a pristine watershed in Illinois

The spatial distribution of exotics across riparian landscapes is not uniform, and research elaborating the environmental constraints and dispersal behavior that underlie these patterns of distribution is warranted. This study examined the spatial distribution, growth patterns, and habitat constraints of populations of the invasive Dioscorea oppositifolia in a forested stream corridor of a tributary of Drury Creek in Giant City State Park, IL. The distribution of D. oppositifolia was determined at the watershed scale mainly by floodplain structure and connectivity. Populations of D. oppositifolia were confined to the floodplain, with overbank flooding from the stream. Dioscorea oppositifolia probably originates in disturbed areas upstream of natural corridors, and subsequently, the species disperses downstream into pristine canyons or ravines via bulbils dispersing in the water. In Giant City State Park, populations of D. oppositifolia were distributed on the floodplain across broad gradients of soil texture, light, slope, and potential radiation. The study also examined the longevity of bulbils in various micro-environments to illuminate strategies for the management of the species in invaded watersheds. After 1 year, the highest percentages of bulbils were viable under leaves, and much lower percentages were viable over leaves, in soil, and in the creek (76.0±6.8, 21.2±9.6, 21.6±3.6, and 5.2±5.2%), respectively. This study suggests that management procedures that reduce leaf litter on the forest floor (e.g., prescribed burning) could reduce the number of bulbils of D. oppositifolia stored in the watershed.

Illinois↗

Life history trade-offs and community dynamics of small fishes in a seasonally pulsed wetland

We used a one-dimensional, spatially explicit model to simulate the community of small fishes in the freshwater wetlands of southern Florida, USA. The seasonality of rainfall in these wetlands causes annual fluctuations in the amount of flooded area. We modeled fish populations that differed from each other only in efficiency of resource utilization and dispersal ability. The simulations showed that these trade-offs, along with the spatial and temporal variability of the environment, allow coexistence of several species competing exploitatively for a common resource type. This mechanism, while sharing some characteristics with other mechanisms proposed for coexistence of competing species, is novel in detail. Simulated fish densities resembled patterns observed in Everglades empirical data. Cells with hydroperiods less than 6 months accumulated negligible fish biomass. One unique model result was that, when multiple species coexisted, it was possible for one of the coexisting species to have both lower local resource utilization efficiency and lower dispersal ability than one of the other species. This counterintuitive result is a consequence of stronger effects of other competitors on the superior species. ?? 2005 NRC.

Conference Paper↗

Effects of nutrient patches and root systems on the clonal plasticity of a rhizomatous grass

Clonal plant foraging has been examined primarily on individual clones exposed to resource-poor and resource-rich environments. We designed an experiment to examine the clonal foraging behavior of the rhizomatous grass Elymus lanceolatus ssp. lanceolatus under the influence of neighboring plant root systems in a heterogeneous nutrient environment. Individual Elymus clones were planted in large bins together with one of three neighboring grass species, Agropyron desertorum, Pseudoroegneria spicata, or Bromus tectorum, which differ in rooting density and growth activity. The position of Elymus clones was manipulated so rhizomes encountered a short-duration nutrient patch and subsequently root systems of the neighboring plants. Unexpectedly, the morphological plasticity of the perennial grass Elymus lanceolatus ssp. lanceolatus was influenced by the presence of the neighboring species much more than by the local nutrient enrichments, although nutrient patches did amplify some of the foraging responses. Elymus rhizomes branched readily and initiated large daughter plants as they encountered the low-density root systems of Pseudoroegneria. When Elymus encountered the fine, dense root systems of the annual Bromus, clonal expansion was initially reduced. Yet, after the short growing season of Bromus, Elymus resumed clonal expansion and produced several daughter plants. Elymus clones were most constrained by the fine, dense root systems of Agropyron desertorum. In this case, a few, long rhizomes avoided the densely rooted soil environment by growing aboveground as stolons crossing over the Agropyron tussocks. Elymus clonal biomass was largest in neighborhoods of Pseudoroegneria, intermediate in neighborhoods with Bromus, and smallest in neighborhoods with Agropyron. The latter were approximately half the size of those in the Pseudoroegneria environments. Elymus growth could not be explained by simple resource competition alone; other mechanisms must have been involved in the apparent differences in interference patterns of neighboring plants with Elymus.

Ecology↗

Earthquake damage to transportation systems

Earthquakes represent one of the most destructive natural hazards known to man. A large magnitude earthquake near a populated area can affect residents over thousands of square kilometers and cause billions of dollars in property damage. Such an event can kill or injure thousands of residents and disrupt the socioeconomic environment for months, sometimes years. A serious result of a large-magnitude earthquake is the disruption of transportation systems, which limits post-disaster emergency response. Movement of emergency vehicles, such as police cars, fire trucks and ambulances, is often severely restricted. Damage to transportation systems is categorized below by cause including: ground failure, faulting, vibration damage, and tsunamis.

Report↗

The interface between rare plant management and genetics: Conserving Pitcher’s thistle (Cirsium pitcheri) in the Great Lakes region, USA

Background and Aims Tools showing best conservation practices are becoming critical for rare plant conservation as populations become isolated through habitat fragmentation and changing ecosystem processes. We illustrate the importance of reintroduction and assisted gene flow, using reintroduction of the USA federally threatened Pitcher’s thistle ( Cirsium pitcheri ), a monocarpic perennial endemic to the western Great Lakes sand dunes. We evaluate the success of experimental reintroductions of this species into its dynamic coastal environment and address fundamental issues for rare plant reintroduction, including questions of maintenance of genetic diversity, founder size, introduction methods and assisted gene flow effectiveness. Methods In 1994 we initiated experimental reintroductions at three new locations along a habitat successional gradient using 4200 seeds collected from 54 maternal lines. At each site, seed sources were distributed among replicate blocks that were split by sowing method (sowing vs broadcasting) to examine establishment success. We monitored population demography for 30 years, assessing genetic variation in 2009 and regionally from 1997 to 2014. Key Results Two of three populations persisted for 30 years from a single-seeding founder event. Reintroduction populations had greater expected heterozygosity than regional native populations. Despite persistence, moderate inbreeding coefficients showed that reintroduction populations have not achieved an effective size to reduce the likelihood of inbreeding depression. However, the mid-successional reintroduction had the lowest kinship of all populations sampled, indicating a healthy restoration. Seed sowing produced three times as many seedlings as broadcasting, but seed source did not affect germination success. Persistence has been facilitated by local migration to suitable habitat patches, an important metapopulation process. Conclusions Seeds can be an effective method for reintroducing monocarpic plants in high-quality habitats. However, low numbers of reproductive adults and moderate inbreeding illustrate the need for repeated additions of plants or seed to reduce inbreeding to improve rare plant genetic evolutionary potential.

Indiana↗

Occupancy modeling species–environment relationships with non‐ignorable survey designs

Statistical models supporting inferences about species occurrence patterns in relation to environmental gradients are fundamental to ecology and conservation biology. A common implicit assumption is that the sampling design is ignorable and does not need to be formally accounted for in analyses. The analyst assumes data are representative of the desired population and statistical modeling proceeds. However, if data sets from probability and non‐probability surveys are combined or unequal selection probabilities are used, the design may be non‐ignorable. We outline the use of pseudo‐maximum likelihood estimation for site‐occupancy models to account for such non‐ignorable survey designs. This estimation method accounts for the survey design by properly weighting the pseudo‐likelihood equation. In our empirical example, legacy and newer randomly selected locations were surveyed for bats to bridge a historic statewide effort with an ongoing nationwide program. We provide a worked example using bat acoustic detection/non‐detection data and show how analysts can diagnose whether their design is ignorable. Using simulations we assessed whether our approach is viable for modeling data sets composed of sites contributed outside of a probability design. Pseudo‐maximum likelihood estimates differed from the usual maximum likelihood occupancy estimates for some bat species. Using simulations we show the maximum likelihood estimator of species–environment relationships with non‐ignorable sampling designs was biased, whereas the pseudo‐likelihood estimator was design unbiased. However, in our simulation study the designs composed of a large proportion of legacy or non‐probability sites resulted in estimation issues for standard errors. These issues were likely a result of highly variable weights confounded by small sample sizes (5% or 10% sampling intensity and four revisits). Aggregating data sets from multiple sources logically supports larger sample sizes and potentially increases spatial extents for statistical inferences. Our results suggest that ignoring the mechanism for how locations were selected for data collection (e.g., the sampling design) could result in erroneous model‐based conclusions. Therefore, in order to ensure robust and defensible recommendations for evidence‐based conservation decision‐making, the survey design information in addition to the data themselves must be available for analysts. Details for constructing the weights used in estimation and code for implementation are provided.

Ecological Applications↗

Surficial sediment character of the New York-New Jersey offshore continental shelf region: A GIS compilation

Broad continental shelf regions such as the New York Bight are the product of a complex geologic history and dynamic oceanographic processes, dominated by the Holocene marine transgression (>100 m sea-level rise) following the end of the last Pleistocene ice advance ~ 20,000 years ago. The area of the U.S. Exclusive Economic Zone (U.S. EEZ) territory, extending 200 nautical miles seaward from the coast, is larger than the continental U.S. and contains submerged landforms that provide a variety of natural functions and societal benefits, such as: critical habitats for fisheries, ship navigation and homeland security, and engineering activities (i.e. oil and gas platforms, pipeline and cable routes, potential wind-energy-generation sites). Some parts of the continental margins, particularly inner-continental shelf regions, also contain unconsolidated hard-mineral deposits such as sand and gravel that are regarded as potential aggregate resources to meet or augment needs not met by onshore deposits (Williams, 1992). The present distribution of surficial sediment off the northeastern United States is shaped from the deposits left by the last glaciation and reflects the cumulative effects of sediment erosion, transport, sorting, and deposition by storm and tidal processes during the Holocene rise in sea level. As a result, the sediments on the sea floor represent both an historical record of former conditions and a guide to possible future sedimentary environments. The U.S. Geological Survey (USGS) through the Coastal and Marine Geology Program, in cooperation with the University of Colorado and other partners, has compiled extant sediment character and textural data as well as other geologic information on the sea floor from all regions around the U.S. into the usSEABED data system (Reid and others, 2005; Buczkowski and others, 2006; Reid and others, 2006). The usSEABED system, which contains information on sediment grain size and lithology for more than 340,500 stations within the U.S. EEZ. has been developed and populated with data as part of the USGS Marine Aggregate Resources and Processes and the National Benthic Habitats projects in order to provide the base-line data needed to update the current maps of offshore surficial geologic character and sediment distribution. The maps are also used to characterize benthic sea floor environments important for marine ecosystems. U.S. Geological Survey, Data Series 118 (Reid and others, 2005), of the usSEABED data release series, represents the combined efforts of the USGS and several other government agencies to provide a unified resource for accessing and preserving records of U.S. east coast sea floor geologic information and sediment texture data. For this present report, we have chosen to focus on the New York-New Jersey region, an area that has been intensely studied by the USGS for many years to address many complex issues. This report illustrates the uses of the usSEABED database for GIS applications, while offering additional insight into the resources and data available from the USGS and its collaborative institutions. This report is based on data contained in U.S. Geological Survey Data Series 118 (Reid and others, 2005) and shows an assortment of example GIS products that are possible using usSEABED. All data are intended to be GIS-ready and should not require any additional cleanup, formatting, or renaming of fields in order to use the data in a Geographic Information System. This project employs the Environmental Systems Research Institute's (ESRI) ArcView™ software. Many of these maps were made as part of the ongoing USGS study to assess marine aggregate resources offshore New York and New Jersey, but these maps can serve many other purposes. The marine science community, educators, students and others are encouraged to use these data to generate GIS products for their own purposes. The objectives of the Marine Aggregate Resources and Processes project are to produce a series of new geologic maps and reports of the sea floor that will provide scientific insights into the character and geologic development of U.S. continental margins and to use these maps and information to assess the potential availability of offshore sand and gravel resources. The mapping and aggregate resource assessments are being conducted on a national scale using the usSEABED data base as described in Williams and others (2003). Potential uses for these data include: (1) defining the geological variability of the sea floor in relation to benthic habitat diversity; (2) improving our understanding of the processes that control the distribution and transport of bottom sediments and benthic habitats; (3) locating aggregate resources for beach nourishment and industrial applications; and (4) providing a detailed geospatial framework for future marine science research, monitoring, and management activities. The initial assessments are in progress for the New York Bight and Louisiana offshore areas.

New Jersey, New York↗

The effects of land use on fluvial sediment chemistry for the conterminous U.S. - Results from the first cycle of the NAWQA Program: Trace and major elements, phosphorus, carbon, and sulfur

In 1991, the U.S. Geological Survey (USGS) began the first cycle of its National Water Quality Assessment (NAWQA) Program. The Program encompassed 51 river basins that collectively accounted for more than 70% of the total water use (excluding power generation), and 50% of the drinking water supply in the U.S. The basins represented a variety of hydrologic settings, rock types (geology), land-use categories, and population densities. One aspect of the first cycle included bed sediment sampling; sites were chosen to represent baseline and important land-use categories (e.g., agriculture, urban) in each basin. In total, over 1200 bed sediment samples were collected. All samples were size-limited (< 63????m) to facilitate spatial and/or temporal comparisons, and subsequently analyzed for a variety of chemical constituents including major (e.g., Fe, Al,) and trace elements (e.g., Cu, Zn, Cd), nutrients (e.g., P), and carbon. The analyses yielded total (??? 95% of the concentrations present), rather than total-recoverable chemical data. Land-use percentages, upstream underlying geology, and population density were determined for each site and evaluated to asses their relative influence on sediment chemistry. Baseline concentrations for the entire U.S. also were generated from a subset of all the samples, and are based on material collected from low population (??? 27??p km- 2) density, low percent urban (??? 5%), agricultural or undeveloped areas. The NAWQA baseline values are similar to those found in other national and global datasets. Further, it appears that upstream/underlying rock type has only a limited effect (mostly major elements) on sediment chemistry. The only land-use category that appears to substantially affect sediment chemistry is percent urban, and this result is mirrored by population density; in fact, the latter appears more consistent than the former.

Science of the Total Environment↗

East versus West: organic contaminant differences in brown pelican ( Pelecanus occidentalis ) eggs from South Carolina, USA and the Gulf of California, Mexico

Brown pelicans ( Pelecanus occidentalis ) were listed as endangered in the United States in 1970, largely due to reproductive failure and mortality caused by organochlorine contaminants, such as DDT. The southeast population, P.o. carolinensis , was delisted in 1985, while the west coast population, P.o. californicus , was not delisted until 2009. As fish-eating coastal seabirds, brown pelicans may serve as a biomonitors. Organic contaminants were examined in brown pelican eggs collected from the Gulf of California in 2004 and South Carolina in 2005 using gas chromatography/mass spectrometry (GC/MS). Contaminants were compared using all individual data as well as statistically pooled samples to provide similar sample sizes with little difference in results. Principal components analysis separated the Gulf of California brown pelican eggs from the South Carolina eggs based on contaminant patterns. The South Carolina population had significantly ( P < 0.05) higher levels of polychlorinated biphenyls (PCBs), chlordanes, dieldrin and mirex, while the Gulf of California eggs had higher levels of dichlorodiphenyltrichloroethanes (DDTs) and hexachlorocyclohexanes (HCHs). With the exception of dieldrin and brominated diphenyl ether (BDE) 47, this pattern was observed for mussel and oyster tissues from these regions, indicating the need for further study into the differences between east and west coast brown pelican populations and ecosystem contamination patterns.

South Carolina↗

Managing dams for energy and fish tradeoffs: What does a win-win solution take?

Management activities to restore endangered fish species, such as dam removals, fishway installations, and periodic turbine shutdowns, usually decrease hydropower generation capacities at dams. Quantitative analysis of the tradeoffs between energy production and fish population recovery related to dam decision-making is still lacking. In this study, an integrated hydropower generation and age-structured fish population model was developed using a system dynamics modeling method to assess basin-scale energy-fish tradeoffs under eight dam management scenarios. This model ran across 150 years on a daily time step, applied to five hydroelectric dams located in the main stem of the Penobscot River, Maine. We used alewife ( Alosa pseudoharengus ) to be representative of the local diadromous fish populations to link projected hydropower production with theoretical influences on migratory fish populations on the model river system. Our results show that while the five dams can produce around 427 GWh/year of energy, without fishway installations they would contribute to a 90% reduction in the alewife spawner abundance. The effectiveness of fishway installations is largely influenced by the size of reopened habitat areas and the actual passage rate of the fishways. Homing to natal habitat has an insignificant effect on the growth of the simulated spawner abundance. Operating turbine shutdowns during alewives' peak downstream migration periods, in addition to other dam management strategies, can effectively increase the spawner abundance by 480–550% while also preserving 65% of the hydropower generation capacity. These data demonstrate that in a river system where active hydropower dams operate, a combination of dam management strategies at the basin scale can best balance the tradeoff between energy production and the potential for migratory fish population recovery.

Maine↗

Water availability drives instream conditions and life-history of an imperiled desert fish: A case study to inform water management

In arid ecosystems, available water is a critical, yet limited resource for human consumption, agricultural use, and ecosystem processes—highlighting the importance of developing management strategies to meet the needs of multiple users. Here, we evaluated how water availability influences stream thermal regimes and life-history expressions of Lahontan cutthroat trout ( Oncorhynchus clarkii henshawi ) in the arid Truckee River basin in the western United States. We integrated air temperature and stream discharge data to quantify how water availability drives stream temperature during annual spawning and rearing of Lahontan cutthroat trout. We then determined how in situ stream discharge and temperature affected adult spawning migrations, juvenile growth opportunities, and duration of suitable thermal conditions. Air temperatures had significant, large effects (+) on stream temperature across months; the effects of discharge varied across months, with significant effects (−) during May through August, suggesting increased discharge can help mitigate temperatures during seasonally warm months. Two models explained adult Lahontan cutthroat trout migration, and both models indicated that adult Lahontan cutthroat trout avoid migration when temperatures are warmer (~ > 12 °C) and discharge is higher (~ > 50 m 3 *s −1 ). Juvenile size was best explained by a quadratic relationship with cumulative degree days (CDD; days>4 °C) as size increased with increasing CDDs but decreased at higher CDDs. We also found an interaction between CDDs and discharge explaining juvenile size: when CDDs were low, higher discharge was associated with larger size, but when CDDs were high, higher discharge was associated with smaller size. Stream temperatures also determined the duration of juvenile rearing, as all juvenile emigration ceased at temperatures >24.4 °C. Together, our results illustrated how stream discharge and temperature shape the life-history of Lahontan cutthroat trout at multiple stages and can inform management actions to offset warming temperatures and facilitate life-history diversity and population resilience.

California, Nevada↗

Presence of avian influenza viruses in waterfowl and wetlands during summer 2010 in California: Are resident birds a potential reservoir?

Although wild waterfowl are the main reservoir for low pathogenic avian influenza viruses (LPAIv), the environment plays a critical role for the circulation and persistence of AIv. LPAIv may persist for extended periods in cold environments, suggesting that waterfowl breeding areas in the northern hemisphere may be an important reservoir for AIv in contrast to the warmer southern wintering areas. We evaluated whether southern wetlands, with relatively small populations (thousands) of resident waterfowl, maintain AIv in the summer, prior to the arrival of millions of migratory birds. We collected water and fecal samples at ten wetlands in two regions (Yolo Bypass and Sacramento Valley) of the California Central Valley during three bi-weekly intervals beginning in late July, 2010. We detected AIv in 29/367 fecal samples (7.9%) and 12/597 water samples (2.0%) by matrix real time Reverse Transcription Polymerase Chain Reaction (rRT-PCR). We isolated two H3N8, two H2N3, and one H4N8 among rRT-PCR positive fecal samples but no live virus from water samples. Detection of AIv RNA in fecal samples was higher from wetlands in the Sacramento Valley (11.9%) than in the Yolo Bypass (0.0%), but no difference was found for water samples (2.7 vs. 1.7%, respectively). Our study showed that low densities of hosts and unfavorable environmental conditions did not prevent LPAIv circulation during summer in California wetlands. Our findings justify further investigations to understand AIv dynamics in resident waterfowl populations, compare AIv subtypes between migratory and resident waterfowl, and assess the importance of local AIv as a source of infection for migratory birds.

California↗

Pesticide residues in the ecosystem

Pesticide residues have become a component of nearly all living organisms. Nearly all California birds and fish collected in a 1963 pesticide survey contained residues. Discovery of DDT and metabolites in Antarctic animals in 1964 pushed the distribution of pesticides to the remotest portions of the globe. Exchange of pesticides in the aquatic world progresses rapidly, even in the quiet waters of a pond. Any segment of the ecosystem–marshland, pond, forest, or field–receives pesticides of varied amounts and kinds at irregular and unknown intervals. Phytoplankton communities are an important food base in aquatic environments whose productivity can be affected by rather small exposure to pesticides. Degradation and loss of chlorinated hydrocarbon pesticides from the ecosystem has been shown to proceed slowly. Pesticide-tolerant or resistant populations of fish and possibly other cold-blooded vertebrates have been shown to exist in areas of the south long subjected to pesticide treatments.

Book chapter↗

Insecticide contaminations in wetland habitats and their effects on fish- eating birds

An unusual mortality of fish-eating birds occurred at the Tule Lake National Wildlife Refuge in California between 1960 and 1962. Over 1100 dead birds of ten species were found during that period. Investigations of the mortality indicated that birds died as a result of their exposure to toxaphene, which was applied to adjoining agricultural areas, transported to the refuge in waste irrigation water, and accumulated in fish eaten by the birds. Toxaphene was used in agriculture for only 3 years, 1958-60, and by 1962 residues were no longer found in the marsh habitat. However, results also showed that a potentially hazardous contamination of DDT and its metabolites was present in the birds and the environment. Studies were, therefore, continued to determine the food-chain relationships of DDT in marsh ecosystems, and the effects of the insecticide on birds. Sampling for insecticide residues was undertaken at four waterfowl refuges, each representing a somewhat different set of environmental conditions. DDT residues in water at each refuge were found to be relatively low, but filtered samples showed that residues in suspended material were 10 000 to 20 000 times as great as those in the filtrate. Much of the suspended material was organic matter, which is an important source of energy for the ecosystems. In environments with invertebrates, DDT residues were relatively low in sediments and high in fish, while residues in vegetation were highest where invertebrates were absent. Results suggest that organic materials and invertebrates can be important factors in determining the fate and involvement of insecticides in aquatic environments. Studies of the movements, exposure to pesticides, productivity, and longevity of individuals in three nesting colonies of White Pelicans were begun in 1962 to depict the relationships of pesticides to a migratory population of birds dependent upon wetland habitats. To date there is no evidence that the exposure of pelicans to DDT is influencing the population dynamics of the birds.

California, Oregon↗