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Weak support for disappearance and restricted emergence/persistence of highly pathogenic influenza A in North American waterfowl

Krauss et al. (1) use lack of detection of highly pathogenic (HP) H5 clade 2.3.4.4 (henceforth "H5") influenza A viruses (IAVs) from >22,000 wild bird samples collected in North America in 2014–2015 to argue that HP H5 IAVs disappeared from waterfowl and that unresolved mechanisms restrict emergence and perpetuation of HP IAVs in natural reservoir species. Here we offer an alternative interpretation.

Proceedings of the National Academy of Sciences↗

Formation and regression of the corpus luteum of the American alligator

Luteal morphology of the American alligator is unique when compared to other reptiles but is similar to that of its phylogenetic relatives, the birds. The theca is extensively hypertrophied, but the granulosa never fills the cavity formed following the ovulation of the ovum. The formation of the corpus luteum (CL) is correlated with elevated plasma progesterone concentrations, which decline dramatically after oviposition with the onset of luteolysis. Unlike those of most other reptiles, the central luteal cell mass is composed of two cell types; one presumably is derived from the granulosa, whereas the other is from the theca interna. Both cell types are present throughout gravidity but only one cell type is seen during mid to late luteolysis. A significant decline in luteal volume occurs following oviposition and continues throughout the post-oviposition period. The fastest decline in luteal volume occurs in the month immediately after oviposition; this rate then slows. Luteolysis appears to continue for a year or more following oviposition, as distinct structures of luteal origin can still be identified in animals 9 months after oviposition. The size of persistent CL can be used to determine whether a given female oviposited during the previous nesting season. Females with CL having volumes greater than 0.2 cm 2 or CL diameters greater than 0.4 cm were active the previous season.

Journal of Morphology↗

Dietary toxicity and tissue accumulation of methylmercury in American kestrels

American kestrels (Falco sparverius) were fed meat diets containing 0, 3, 6, or 12 ppm (dry weight) methylmercury chloride. Birds fed the 12-ppm diet started to show signs of neurotoxicity after 26 days and all died in 39?49 days. One male kestrel fed the 6-ppm diet died after 75 days of exposure and several others showed signs of neurotoxicity after 45 days. None of the birds fed the 3-ppm diet died or showed signs of toxicity. After 59 days of exposure, mercury concentrations in the liver, kidney, and blood of nonreproducing kestrels increased with increasing dietary concentration. Tissue concentrations of mercury also steadily increased over time in birds fed diets with 6 ppm mercury, which were necropsied at 8, 15, 29, or 59 days of exposure, reaching mean total mercury concentrations of 57, 46, and 45 ppm (wet weight) at 59 days in the liver, kidney, and whole blood, respectively. Two pairs of kestrels at each dietary concentration were allowed to breed. Eggs averaged 8.3 and 18.1 ppm (wet weight) total mercury from birds fed 3- and 6-ppm diets, respectively. Feathers grown during mercury exposure contained high concentrations of mercury: Birds fed 3- and 6-ppm diets contained 275 and 542 ppm total mercury, respectively.

Archives of Environmental Contamination and Toxico↗

Non-random pairing in American kestrels: mate choice versus intra-sexual competition

Natural selection may influence the arrangement of individuals into mated pairs through either inter-sexual (mate choice) or intra-sexual selection (competition). A study of the American kestrel, Falco sparverius , in northern Saskatchewan distinguished between these two processes using size as a measure of the bird's competitive ability, and condition (mass scaled to body size) as an index of quality. Both sexes arrive on the study area after spring migration in equal numbers and males establish territories. Males and females that moved among territories at the time of pair formation were not different in size or condition from those that did not move, suggesting that birds were not being displaced by superior competitors, and that females moved to encounter potential mates. Within mated pairs, there was no relationship between a bird's size and the condition of its mate for either sex as would be predicted if intra-sexual competitition explained mating patterns. Instead, there was positive assortative mating by condition, suggesting that both sexes used quality as the criterion in choosing mates. There was no correlation between the sizes of males and females in mated paird. Because there were no differences in size or condition of breeding and non-breeding males, factors other than physical attributes, such as prior experience with the area, may determine a male's success in obtaining a territory. Because females that did not obtain mates were in poorer condition than those that did, males may have rejected poor quality females. The results suggest that intra-sexual competition was not important for pair formation, and that kestrels chose mates on the basis of quality.

Animal Behaviour↗

Feeding ecology of long-tailed ducks Clangula hyemalis wintering on the Nantucket Shoals

A substantial proportion, perhaps 30%, of the North American breeding population of Long-tailed Ducks ( Clangula hyemalis ) winter in the vicinity of Nantucket Island, Massachusetts. These birds spend the night on Nantucket Sound and commute during daylight hours to the Nantucket Shoals, which extend about 65 km offshore from the southeastern corner of Nantucket. Strip transects done from a single-engine plane in 1997 and 1998 indicated that Long-tailed Ducks foraged over the shallower (≤ 20 m depth) portions of the Nantucket Shoals, up to 70 km offshore. Diet analyses of ten birds collected in February 1999 and five in December 2006 showed that they fed principally (106.6 /- 42.0 individuals per crop) on Gammarus annulatus , a pelagic amphipod that often forms large aggregations, and is consumed by several species of fish and marine mammals. Our findings emphasize the importance of conservation of the Nantucket Shoals and the prevention of oil spills or other potentially harmful accidents.

Massachusetts↗

Sex-specific differences in winter distribution patterns of canvasbacks

Winter band recovery distributions of North American Canvasbacks (Aythya valisineria) suggested that males and females exhibit comparable degrees of fidelity to general wintering areas. Of birds banded during the winter, the proportion of males was found to be higher in northern than in southern areas. Winter band recovery distributions of birds banded in particular areas during the summer were found to differ significantly between sexes, with females being recovered farther south. Factors that may have affected the evolution of sex-specific wintering distributions include: (1) possible reproductive benefits derived by males who winter in the north and thus reach northerly breeding areas early; (2) sexual dimorphism in body size, which may render the smaller females especially susceptible to periods of inclement weather and food shortages; and (3) interactions between sexes in which males may control food supply when food is scarce. Two lines of evidence from field data on Canvasbacks in the Chesapeake Bay suggest the existence of competition between males and females. First, Canvasbacks trapped during winter in smaller bodies of water tended to have higher proportions of females and weigh less than birds trapped in large open bodies of water. Second, analysis of aerial photographs of wintering rafts of Canvasbacks showed patterns of intersexual segregation, with females being found more frequently on peripheral areas of rafts.

Condor↗

Nest-site characteristics and linear abundance of cliff-nesting American kestrels on San Clemente Island, California

American Kestrels( Falco sparverius) are typically secondary-cavity nesters, and use of natural cliff cavities for nest sites is less-commonly reported. On San Clemente Island (SCI), California, however, American Kestrels nest primarily on cliffs in major canyons(93%), to a lesser extent on seacliffs(4%), as well as in man-made structures (3%). We located and mapped 99 American Kestrel territories on SCI, and recorded 11 nest-site characteristics at 40 cliff nests during 2001-02. Nest cliffs were typically fractured igneous rock with mean height of 16.1 m +_ 1.8 SE. Mean slope of nest cliffs was vertical (x=91 degrees). Nest cliffs and cavities were significantly oriented to the southeast, away from the prevailing wind direction(NW). In eight canyons, where we believe that we found all occupied American Kestrel territories, the mean linear abundance was 2.1 pairs/km, greater than most published estimates. Contrary to most previous studies, no American Kestrels nested in tree cavities despite their presence in SCI canyons. The absence of cavity-excavating breeding birds from the island likely restricts kestrels to nesting in naturally-formed cavities and man-made structures.

Journal of Raptor Research↗

Shorebirds: East of the 105th meridian

The North American group of shorebirds includes 48 kinds of sandpipers, plovers, and their allies, many of which live for most of the year in coastal marine habitats; other live principally in nonmarine habitats including grasslands, freshwater wetlands, and even second-growth woodlands. Most North American shorebirds are highly migratory, while others are weakly migratory, and even nonmigratory in some parts of their range. Here we discuss shorebirds east of the 105th meridian (roughly east of the Rocky Mountains). Historically, populations of many North American species were dramatically reduces by excessive gunning (Forbush 1912). Most populations recovered after the passage of the Migratory Bird Treaty Act of 1918, although some species never recovered and others have declined again. High proportions of entire populations of shorebirds migrate by visiting one or a small number of "staging sites", areas where the birds accumulate fat to provide fuel before continuing with their long-distance, nonstop flights to the next site (Morrison and Harrington 1979; Senner and Howe 1984; Harrington et al. 1991). Growing evidence (Schneider and Harrington 1981) indicates that staging areas are unusually productive sites with highly predictable but seasonally ephemeral "blooms" of invertebrates, which shorebirds use for fattening. In some cases, especially for "obligate" coastal species, specific sites are traditionally used; even other species sites may shift between years. Because pf this, conservationists believe some species are at risk through loss of strategic migration site (Myers et al. 1987). Other species are threatened by the less of breeding and wintering habitats (Page et al. 1991; Haig and Plissner 1993; B. Leachman and B. Osmundsom, U.S. Fish and Wildlife Service, unpublished data). The predicted consequences of global warming, such as sea-level change, will also strongly affect the intertidal marine habitats, which many species of shorebirds depend upon. Some of the strongest warming effects will be at high latitudes, including those where many shorebirds migrate to breed, as well as south temperate latitudes, where many of them winter.

Book chapter↗

Breeding bird response to cattle grazing of a cottonwood bottomland

We studied avian habitat relationships and the impact of grazing on breeding densities of selected migratory birds in a plains cottonwood ( Populus sargentii ) bottomland in northeastern Colorado. Five 16-ha plots served as controls and 5 were fenced and fall-grazed October-November 1982-84 following a season of pre-treatment study in the spring of 1982. We focused our analysis on bird species directly dependent on the grass-herb-shrub layer of vegetation for foraging, nesting, or both. The guild included house wren ( Troglodytes aedon ), brown thrasher ( Toxostoma rufum ), American robin ( Turdus migratorius ), common yellowthroat ( Geothlypis trichas ), yellow-breasted chat ( Icteria virens ), and rufous-sided towhee ( Pipilo erythropthalmus ). Moderate, late-fall grazing had no detectable impact on calculated densities of any of the 6 species, implying that proper seasonal grazing of a cottonwood floodplain is, at least initially (3 years), compatible with migratory bird use of a site for breeding. Habitat associations suggested that common yellowthroats and yellow-breasted chats were most unique and most likely to respond negatively to higher levels of grazing. We suggest that these latter 2 species are appropriate ecological indicators of the quality of ground-shrub vegetation as breeding bird habitats in lowland floodplains of the Great Plains.

Journal of Wildlife Management↗

Sources of variation in survival and recovery rates of American black ducks

Band recovery data from 10 preseason and 10 winter populations of the American black duck ( Anas rubripes ) were analyzed and survival and recovery rates estimated. Adults showed higher survival rates and lower recovery rates than 1st-year birds. Higher harvest rates of juveniles probably explain much of the age-related mortality differential, but higher vulnerability to nonhunting mortality factors probably also plays a role. Adult males had higher survival rates than adult females but recovery rates were similar to those of adult females. Young males and females had similar survival rates, but recovery rates of young males were higher than those of young females. Further analysis and monitoring of the American black duck population is needed to evaluate the efficacy of current management.

Journal of Wildlife Management↗

Accumulation in and effects of lead and cadmium on waterfowl and passerines in northern Idaho

Waterfowl and passerines in northern Idaho in 1987 had high levels of lead in their blood and tissues that originated primarily from mining and smelting activities. Four Canada geese ( Branta canadensis ) and one common goldeneye ( Bucephala clangula ) found dead contained 8 to 38 μg/g (wet mass) of lead in their livers. These levels exceed the lower lethal limit of 5 μg/g in experimental birds. Two of the Canada geese (one each from the contaminated and reference areas) died with ingested lead shotgun pellets (shot) in their gizzards, whereas the other three birds from the contaminated area contained no ingested shot and evidently died from ingesting environmental lead in sediment or biota. Lead burdens in most American robins ( Turdus migratorius ) and mallards ( Anas platyrhynchos ) were high, whereas those in tree swallows ( Tachycineta bicolor ) were slightly elevated. Lead accumulated to potentially hazardous levels in blood and tissues of some nestling robins (maxima of 0·87 μg/g in blood and 5·6 μg/g in liver) and mallards (maxima of 10·2 μg/g in blood and 2·8 μg/g in liver). In mallards, lead levels and associated physiological characteristics of blood were significantly different in juveniles (HY) versus adults (AHY). Activity of δ-aminolevulinic acid dehydratase (ALAD) was about 87 to 95% lower than values for control birds in experimental studies. Activity of ALAD was significantly inversely correlated with blood lead levels. Cadmium was detected in kidneys of most birds, but even the maximum concentration of 7·5 μg/g in an AHY mallard was below known harmful levels.

Idaho↗

Disseminated granulomas caused by an unidentified protozoan in sandhill cranes

Oral granulomas were observed in 31 (33%) of 95 captive sandhill cranes (Grus canadensis) at the Patuxent Wildlife Research Center. Necropsy of six of the afflicted cranes revealed granulomatous nodules throughout many of their organ systems. Intracellular protozoan organisms morphologically resembling schizogonic stages were observed within the granulomas by light and electron microscopy. Sexual and asexual stages of coccidia were seen in sections of the intestines of 4 of 5 cranes examined microscopically, and Eimerian oocysts were seen in fecal flotation specimens from 3 of 4 birds.

Journal of the American Veterinary Medical Associa↗

Leucism in a family group and a review of color aberrations in Burrowing Owls ( Athene cunicularia )

Avian color aberrations capture public interest and sometimes indicate ecological problems. Diagnosing color aberration type can be difficult because nomenclature is inconsistent, appearance of color aberrations vary, typical coloration varies, and there are many undocumented color aberration types. One type of recognized avian color aberration is leucism. Leucism is often colloquially used to refer to all birds that are paler than normal but not albino, but it specifically refers to a plumage aberration in which, from hatching onward, birds are either all white or have patches of bilaterally symmetrical white feathers mixed with normally colored feathers, while retaining black pupils. Here, we document a case of leucism in a family of North American Burrowing Owls ( Athene cunicularia ). We observed a family group (two adults and five juveniles) of five leucistic, nearly all white owls, and two typically colored owls, in the Conata Basin, South Dakota, USA, in summer 2024. We also compiled all searchable records of color aberrations in Burrowing Owls across their North and South American range. We found 60 records of color aberrations, seven of which were cases of potential leucism. Twenty-five color aberrations were an unknown type in which only eye color was affected, all of them in Florida (USA) or south/central Brazil. Documenting color aberrations in birds can help expand our understanding of the types, causes, and consequences of color aberrations as well as the pigments and genetics that underly normal coloration.

South Dakota↗

Evaluation of thermal antinociceptive effects after intramuscular administration of hydromorphone hydrochloride to American kestrels ( Falco sparverius )

Objective —To evaluate the antinociceptive and sedative effects and duration of action of hydromorphone hydrochloride after IM administration to American kestrels ( Falco sparverius ). Animals —11 healthy 2-year-old American kestrels. Procedures —Hydromorphone (0.1, 0.3, and 0.6 mg/kg) and an equivalent volume of saline (0.9% NaCl) solution (control treatment) were administered IM to kestrels in a masked randomized complete crossover study design. Foot withdrawal response to a thermal stimulus was determined 30 to 60 minutes before (baseline) and 0.5, 1.5, 3, and 6 hours after treatment administration. Agitation-sedation scores were determined 3 to 5 minutes before each thermal test. Results —Hydromorphone at 0.6 mg/kg, IM, significantly increased the thermal foot withdrawal threshold, compared with the response after administration of saline solution, for up to 3 hours, and hydromorphone at 0.1, 0.3, and 0.6 mg/kg, IM, significantly increased withdrawal responses for up to 6 hours, compared with baseline values. No significant differences in mean sedation-agitation scores were detected between hydromorphone and saline solution treatments; however, appreciable sedation was detected in 4 birds when administered 0.6 mg of hydromorphone/kg. Conclusions and Clinical Relevance —Hydromorphone at the doses evaluated significantly increased the thermal nociception threshold for American kestrels for 3 to 6 hours. Additional studies with other types of stimulation, formulations, dosages, routes of administration, and testing times are needed to fully evaluate the analgesic and adverse effects of hydromorphone in kestrels and other avian species and the use of hydromorphone in clinical settings.

American Journal of Veterinary Research↗

Avian eggshell thickness in relation to egg morphometrics, embryonic development, and mercury contamination

Eggshell thickness is important for physiological, ecological, and ecotoxicological studies on birds; however, empirical eggshell thickness measurements for many species and regions are limited. We measured eggshell thickness at the equator and the egg poles for 12 avian species and related eggshell thickness to egg morphometrics, embryonic development, egg status, and mercury contamination. Within an egg, eggshells were approximately 5.1% thicker at the equator than the sharp pole of the egg, although this difference varied among species (0.6%–9.8%). Within Forster's tern ( Sterna forsteri ), where eggshell thickness was measured at 5 equally spaced positions along the longitude of the egg, eggshell thickness changed more rapidly near the sharp pole of the egg compared to near the blunt pole of the egg. Within species, eggshell thickness was related to egg width and egg volume for six of the 12 species but was not related to egg length for any species. Among species, mean eggshell thickness was strongly related to species mean egg width, egg length, egg volume, and bird body mass, although species mean body mass was the strongest predictor of species mean eggshell thickness. Using three species (American avocet [ Recurvirostra americana ], black‐necked stilt [ Himantopus mexicanus ], and Forster's tern), whose nests were carefully monitored, eggshell thickness (including the eggshell membrane) did not differ among viable, naturally abandoned, dead, or failed‐to‐hatch eggs; was not related to total mercury concentrations of the egg content; and did not decrease with embryonic age. Our study also provides a review of all existing eggshell thickness data for these 12 species.

Ecology and Evolution↗

Waterbird nest-site selection is influenced by neighboring nests and island topography

Avian nest-site selection is influenced by factors operating across multiple spatial scales. Identifying preferred physical characteristics (e.g., topography, vegetation structure) can inform managers to improve nesting habitat suitability. However, social factors (e.g., attraction, territoriality, competition) can complicate understanding physical characteristics preferred by nesting birds. We simultaneously evaluated the physical characteristics and social factors influencing selection of island nest sites by colonial-nesting American avocets ( Recurvirostra americana ) and Forster's terns ( Sterna forsteri ) at 2 spatial scales in San Francisco Bay, 2011–2012. At the larger island plot (1 m 2 ) scale, we used real-time kinematics to produce detailed topographies of nesting islands and map the distribution of nests. Nesting probability was greatest in island plots between 0.5 m and 1.5 m above the water surface, at distances <10 m from the water's edge, and of moderately steep (avocets) or flat (terns) slopes. Further, avocet and tern nesting probability increased as the number of nests initiated in adjacent plots increased up to a peak of 11–12 tern nests, and then decreased thereafter. Yet, avocets were less likely to nest in plots adjacent to plots with nesting avocets, suggesting an influence of intra-specific territoriality. At the smaller microhabitat scale, or the area immediately surrounding the nest, we compared topography, vegetation, and distance to nearest nest between nest sites and paired random sites. Topography had little influence on selection of the nest microhabitat. Instead, nest sites were more likely to have vegetation present, and greater cover, than random sites. Finally, avocet, and to a lesser extent tern, nest sites were closer to other active conspecific or heterospecific nests than random sites, indicating that social attraction played a role in selection of nest microhabitat. Our results demonstrate key differences in nest-site selection between co-occurring avocets and terns, and indicate the effects of physical characteristics and social factors on selection of nesting habitat are dependent on the spatial scale examined. Moreover, these results indicate that islands with abundant area between 0.5 m and 1.5 m above the water surface, within 10 m of the water's edge, and containing a mosaic of slopes ranging from flat to moderately steep would provide preferred nesting habitat for avocets and terns. © 2016 The Wildlife Society.

California↗

Avian influenza at both ends of a migratory flyway: characterizing viral genomic diversity to optimize surveillance plans for North America

Although continental populations of avian influenza viruses are genetically distinct, transcontinental reassortment in low pathogenic avian influenza (LPAI) viruses has been detected in migratory birds. Thus, genomic analyses of LPAI viruses could serve as an approach to prioritize species and regions targeted by North American surveillance activities for foreign origin highly pathogenic avian influenza (HPAI). To assess the applicability of this approach, we conducted a phylogenetic and population genetic analysis of 68 viral genomes isolated from the northern pintail (Anas acuta) at opposite ends of the Pacific migratory flyway in North America. We found limited evidence for Asian LPAI lineages on wintering areas used by northern pintails in California in contrast to a higher frequency on breeding locales of Alaska. Our results indicate that the number of Asian LPAI lineages observed in Alaskan northern pintails, and the nucleotide composition of LPAI lineages, is not maintained through fall migration. Accordingly, our data indicate that surveillance of Pacific Flyway northern pintails to detect foreign avian influenza viruses would be most effective in Alaska. North American surveillance plans could be optimized through an analysis of LPAI genomics from species that demonstrate evolutionary linkages with European or Asian lineages and in regions that have overlapping migratory flyways with areas of HPAI outbreaks.

Alaska, California↗