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The importance of the riparian zone and in-stream processes in nitrate attenuation in undisturbed and agricultural watersheds – a review of the scientific literature

We reviewed published studies from primarily glaciated regions in the United States, Canada, and Europe of the (1) transport of nitrate from terrestrial ecosystems to aquatic ecosystems, (2) attenuation of nitrate in the riparian zone of undisturbed and agricultural watersheds, (3) processes contributing to nitrate attenuation in riparian zones, (4) variation in the attenuation of nitrate in the riparian zone, and (5) importance of in-stream and hyporheic processes for nitrate attenuation in the stream channel. Our objectives were to synthesize the results of these studies and suggest methodologies to (1) monitor regional trends in nitrate concentration in undisturbed 1st order watersheds and (2) reduce nitrate loads in streams draining agricultural watersheds. Our review reveals that undisturbed headwater watersheds have been shown to be very retentive of nitrogen, but the importance of biogeochemical and hydrological riparian zone processes in retaining nitrogen in these watersheds has not been demonstrated as it has for agricultural watersheds. An understanding of the role of the riparian zone in nitrate attenuation in undisturbed watersheds is crucial because these watersheds are increasingly subject to stressors, such as changes in land use and climate, wildfire, and increases in atmospheric nitrogen deposition. In general, understanding processes controlling the concentration and flux of nitrate is critical to identifying and mapping the vulnerability of watersheds to water quality changes due to a variety of stressors. In undisturbed and agricultural watersheds we propose that understanding the importance of riparian zone processes in 2nd order and larger watersheds is critical. Research is needed that addresses the relative importance of how the following sources of nitrate along any given stream reach might change as watersheds increase in size and with flow: (1) inputs upstream from the reach, (2) tributary inflow, (3) water derived from the riparian zone, (4) groundwater from outside the riparian zone (intermediate or regional sources), and (5) in-stream (hyporheic) processes.

Journal of Hydrology

A review of the literature on the use of TFM-Bayluscide in fisheries

Since 1956 the Great Lakes Fishery Commission has been responsible for formulating and implementing a program to eradicate or control the sea lamprey in the Great Lakes. The U.S. Fish and Wildlife Service and the Sea Lamprey Control Centre of the Canadian Department of the Environment act as agents for the Commission in sea lamprey control. In the search for a selective lampricide that would control lampreys without destroying fish and other aquatic organisms, about 6,000 chemicals were tested at the Hammond Bay Biological Station of the U.S. Fish and Wildlife Service, largely during the mid 1950's. One compound, TFM, which is selectively toxic to sea lampreys was developed for field use. In 1963 Bayluscide was discovered to be extremely toxic to sea lampreys. Because Bayluscide is also very toxic to fish, being virtually nonselective between lampreys and rainbow trout, only 3% by weight can be added to TFM without losing the selective toxicity of TFM. Addition of small amounts of Bayluscide to TFM, however, effects substantial savings by greatly reducing the amount of TFM needed for effective treatment of lamprey populations. Since 1963 mixtures of TFM and Bayluscide have been used as a lampricide in tributaries of the Great Lakes by both the U.S. and Canadian governments.

Literature Review

Evidence gaps and diversity among potential win–win solutions for conservation and human infectious disease control

As sustainable development practitioners have worked to “ensure healthy lives and promote well-being for all” and “conserve life on land and below water”, what progress has been made with win–win interventions that reduce human infectious disease burdens while advancing conservation goals? Using a systematic literature review, we identified 46 proposed solutions, which we then investigated individually using targeted literature reviews. The proposed solutions addressed diverse conservation threats and human infectious diseases, and thus, the proposed interventions varied in scale, costs, and impacts. Some potential solutions had medium-quality to high-quality evidence for previous success in achieving proposed impacts in one or both sectors. However, there were notable evidence gaps within and among solutions, highlighting opportunities for further research and adaptive implementation. Stakeholders seeking win–win interventions can explore this Review and an online database to find and tailor a relevant solution or brainstorm new solutions.

The Lancet Planetary Health

Flood-frequency estimation for very low annual exceedance probabilities using historical, paleoflood, and regional information with consideration of nonstationarity

Streamflow estimates for floods with an annual exceedance probability of 0.001 or lower are needed to accurately portray risks to critical infrastructure, such as nuclear powerplants and large dams. However, extrapolating flood-frequency curves developed from at-site systematic streamflow records to very low annual exceedance probabilities (less than 0.001) results in large uncertainties in the streamflow estimates. Traditionally, methods for statistically estimating flood frequency have relied on the systematic streamflow record, which provides a time series of annual maximum flood peaks, often including some historical peaks. However, most peak-flow records are less than 100 years, and uncertainties are large when trying to extrapolate magnitudes of very low annual exceedance probability events. Other data may be available that extend the record beyond the systematic dataset. Historical data are defined as data from outside the period of systematic records but within the period of human records. Examples of historical information include flood estimates from other agencies and newspaper accounts that can be translated to flood magnitude point estimates, interval estimates, or perception thresholds (such as a statement that an 1880 flood was the largest since 1869). Paleoflood data, which may also extend the dataset, include a broad range of information about flood occurrence or magnitude from sources like sediment deposits or tree rings. Several assumptions are made in flood-frequency analysis, and an understanding of whether the data conform to these assumptions is desired. A particularly difficult assumption to evaluate for flood-frequency analysis is the underlying assumption that the flood series is stationary—the assumption that a time series of peak flow varies around a constant mean within a particular range of values (constant variance). As the hydrologic community’s understanding of natural systems and anthropogenic effects on streamflows has evolved, the community has come to understand that many surface-water systems exhibit one or more forms of nonstationarity, and thus the stationarity assumption is often violated to some degree. However, there is currently (2020) no consensus among hydrologists regarding the most appropriate flood-frequency-analysis methods for nonstationary systems, and this topic remains an active area of research. A literature review was completed to summarize the state of the science of flood frequency. The literature review highlights tools available to detect nonstationarities and identifies approaches that include external information to inform flood-frequency analysis. To demonstrate methods for initial data analysis and for incorporating historical and paleoflood information in flood-frequency analysis, five sites were selected: the Red River of the North at James Avenue Pumping Station, Winnipeg, Manitoba, Canada; lower reach, Rapid Creek, South Dakota; Spring Creek, South Dakota; Cherry Creek near Melvin, Colorado; and Escalante River near Escalante, Utah. The sites were chosen for the availability of published historical and paleoflood data and for their geographic diversity and unique characteristics, which highlighted issues such as autocorrelation, change points, trends, outlier peaks, or short periods of record. An initial data analysis that involved examining records for autocorrelation, change points, and trends was completed for all sites. The flood-frequency analysis completed for this study used version 7.2 of the U.S. Geological Survey PeakFQ program. Multiple analyses were done on each site documenting the change in the flood-frequency curve when additional historical or paleoflood data were added. When other flood-frequency studies were available, their results were compared to the results here. The comparisons in some cases simply show the effect of additional years of data, whereas other comparisons show results from probability distributions or fitting methods other than those used in PeakFQ. For the Red River of the North, flood-frequency analysis shows that paleoflood data appear necessary to reasonably estimate very low annual exceedance probabilities. For the analysis of the lower reach of Rapid Creek and Spring Creek, paleoflood information helped put a high outlier from the systematic period in context; however, very low annual exceedance probabilities at these sites still had extraordinarily large confidence bounds. These sites also showed that paleoflood information might be transferred from one site to another, with the caveat that this is a case where we had existing paleoflood data to test the transfer of paleoflood information—this is not the case at many sites, and transferring paleoflood information requires assumptions about the comparability of floods at the sites. The Cherry Creek analysis affirmed the result of an earlier study that showed that the generalized Pareto distribution was not a good distribution for estimating very low annual exceedance probabilities. The Escalante River analysis showed that adding paleoflood information might increase uncertainty for very low annual exceedance probabilities, compared to analysis with the systematic period of record information only, when the paleoflood peaks are of much larger magnitudes than the systematic record.

Scientific Investigations Report

Impacts of periodic dredging on macroinvertebrate prey availability for benthic foraging fishes in central San Francisco Bay, California

Background Because of its importance for species covered under Federal Fishery Management Plans (FMPs), the San Francisco Bay (SFB) estuary has been designated as Essential Fish Habitat (EFH) under the Magnuson-Stevens Fishery Conservation and Management Act (MSA; 16 United States Code §18559b). Within this estuary, benthic macroinvertebrate communities provide important prey resources for many economically significant fish species that rely on EFH. Periodic maintenance dredging can impact benthic communities; however, there is a lack of scientific information specific to SFB regarding dredging effects on macroinvertebrates in fish foraging areas. In addition, rates of benthic community recolonization and recovery following dredging and subsequent effects on foraging fish are unknown. For this reason, it is difficult for regulatory and resource agencies to determine the impacts of maintenance dredging. Thus, the National Marine Fisheries Service (NMFS) and the consortium of agencies (U.S. Environmental Protection Agency [EPA], U.S. Army Corp of Engineers [USACE], San Francisco Regional Water Quality Control Board [SFRWQCB], and San Francisco Bay Conservation and Development Commission [BCDC]) that make up the San Francisco Bay Long Term Management Strategy for Dredging (LTMS) identified a study of dredging impacts on SFB fish foraging habitat as one of their highest priorities in their 2011 Programmatic EFH Agreement (U.S. Army Corp of Engineers and U.S. Environmental Protection Agency, 2011). The LTMS agencies identified the region of interest as shallow (<13 feet [<4 meters (m)] mean lower low water [MLLW]), soft-bottom (silt/clay soil texture) areas in the Central Bay of SFB that were periodically dredged (every 1–3 years). Fish species of interest were compiled by NMFS and included those managed by the Pacific Groundfish, Pacific Salmon, and Coastal Pelagic FMPs (pursuant to the MSA) as well as those listed under the California State or Federal Endangered Species Act (ESA; 16 U.S.C. §1531–1544) as threatened or endangered. Target species included leopard shark ( Triakis semifasciata ), big skate ( Raja binoculata ), English sole ( Parophrys vetulus ), starry flounder ( Platichthys stellatus) , brown rockfish ( Sebastes auriculatus ), green sturgeon ( Acipenser medirostris ; threatened species under Federal ESA), northern anchovy ( Engraulis mordax ), longfin smelt ( Spirinchus thaleichthys, threatened under California ESA), and Pacific sardine ( Sardinops sagax ). In addition, Dungeness crab ( Cancer magister ), California halibut ( Paralichthys californicus ), and white sturgeon ( Acipenser transmontanus ) also were included because they are substantial contributors to the California State fishery. To address LTMS priorities, U.S. Geological Survey, Western Ecological Research Center, San Francisco Bay Estuary Field Station (hereafter USGS) conducted a multi-phased project including an initial literature review, study design, pilot study, and implementation of a full study. The overarching goal was to assess the effects of periodic dredge operations (every 1–3 years) on benthic habitat for foraging fish in the Central Bay, with emphasis on the foraging requirements of target fish species and analyses of benthic macroinvertebrates in dredged areas compared to adjacent undredged reference areas. The USGS partnered with University of California, Davis, fisheries expert James Hobbs to synthesize existing knowledge of fish foraging ecology and review benthic infauna community composition in SFB with a focus on the Central Bay. The literature review (Phase I; De La Cruz and others, 2016) addressed key questions identified by the LTMS on benthic foraging fish in the study area, including the following: (1) What are target fish eating? (2) What are the seasonal differences in prey items and macroinvertebrate assemblages? (3) What are the annual differences in prey items and macroinvertebrate assemblages? (4) What are the predominant macroinvertebrate functional groups from the perspective of fish foraging? Phase II consisted of creating a framework for a functional assessment of maintenance dredging effects on foraging fish and drafting a full study design (De La Cruz and others, 2017), which was then tested in the Phase III pilot study. The Phase IV full study incorporated lessons learned from the pilot study. Here we focus on the results of the full study and implications for benthic foraging fishes.

California

Workshops report for mesophotic and deep benthic community fish, mobile invertebrates, sessile invertebrates and infauna

Two workshops with subject matter experts in the appropriate fields, were held in November and December 2021 to elicit guidance and feedback from the broader mesophotic and deep benthic scientific community. These workshops focused on best practices/approaches and identifying data gaps relative to habitat assessment and evaluation goals of the Mesophotic and Deep Benthic Community (MDBC) restoration portfolio. The first workshop was a combined effort of the Habitat Assessment and Evaluation (HAE) Project Team and the Deepwater Horizon (DWH) Program. Industrial Economics, Inc. (IEc) provided extensive workshop planning, organizing, execution, and facilitation support during all stages of the workshop. Based on a questionnaire sent to scientists in August, 2021, the workshop focused on fish and mobile invertebrate habitat associations, abundance trends, community metrics, and food web functionality. Topical presentations and discussions focused not only on demersal fish and mobile invertebrates that are directly associated with mesophotic and deep benthic habitats, but also considered water column species and communities that benefit from these habitats more broadly. The second workshop, intended to complement the first workshop, focused on identifying best practices and critical information gaps for key community metrics, larval dispersal modeling, connectivity, effects and variability of environmental parameters, and recovery trajectories of corals, infauna, and other sessile invertebrates. Through literature review, internal HAE scientists considered these topics to be critical for restoration success. Products from the literature review included topical summaries (see Appendix B) that summarized the current state-of-the-science and provided the framework for the workshop. Information generated from the workshops will assist the MDBC HAE Project, and more broadly the DWH Program, identify data gaps and develop a suite of best practices for restoration activities.

DWH MDBC Summary Report

Assessment of a habitat equivalency analysis for freshwater mussels in the upper Mississippi River

The upper Mississippi River (UMR) contains diverse, dense, and reproducing assemblages of native freshwater mussels. In the case of an injury to mussels and their habitats, such as a hazardous material spill, train derailment, or barge grounding, resource managers have few restoration strategies. Resource managers need a means to document, quantify, and mitigate adverse effects on mussels resulting from injury. Habitat equivalency analysis (HEA), developed for use with a wide variety of habitat types, is a restoration scaling technique that compares ecological services lost from injury to ecological services gained through restoration actions. The U.S. Fish and Wildlife Service and Iowa Department of Natural Resources modified the HEA for use with native mussels. The mussel HEA has been applied within the UMR to estimate the quantity of restoration needed to compensate the public for injuries to mussels due to contaminant spills and construction projects. Our objective was to describe the UMR HEA for a general audience and assess if the four biological input variables used in the mussel HEA were reasonable based on literature values. We also evaluated the performance of HEA under a range of input scenarios. Although the input estimates used in HEA were within ranges reported in the peer-reviewed literature or were supported by professional judgment in the absence of peer-reviewed literature, outcomes of the mussel HEA were highly variable and would benefit from additional research to reduce uncertainty in the biological inputs. The application of HEA to mussels provides resource managers with a tool to quantify mussel-related ecological services lost from injury and to guide restoration efforts in the UMR.

Freshwater Mollusk Biology and Conservation

Inventory of Amphibians and Reptiles at Mojave National Preserve: Final Report

As part of the National Park Service Inventory and Monitoring Program in the Mojave Network, we conducted an inventory of amphibians and reptiles at Mojave National Preserve in 2004-2005. Objectives for this inventory were to use fieldwork, museum collections, and literature review to document the occurrence of reptile and amphibian species occurring at MOJA. Our goals were to document at least 90% of the species present, provide one voucher specimen for each species identified, provide GIS-referenced distribution information for sensitive species, and provide all deliverables, including NPSpecies entries, as outlined in the Mojave Network Biological Inventory Study Plan. Methods included daytime and nighttime visual encounter surveys and nighttime road driving. Survey effort was concentrated in predetermined priority sampling areas, as well as in areas with a high potential for detecting undocumented species. We recorded 31 species during our surveys. During literature review and museum specimen database searches, we found records for seven additional species from MOJA, elevating the documented species list to 38 (two amphibians and 36 reptiles). Based on our surveys, as well as literature and museum specimen review, we estimate an overall inventory completeness of 95% for Mojave National Preserve herpetofauna; 67% for amphibians and 97% for reptiles.

Open-File Report

Inventory of amphibians and reptiles at Death Valley National Park

As part of the National Park Service Inventory and Monitoring Program in the Mojave Network, we conducted an inventory of amphibians and reptiles at Death Valley National Park in 2002-04. Objectives for this inventory were to: 1) Inventory and document the occurrence of reptile and amphibian species occurring at DEVA, primarily within priority sampling areas, with the goal of documenting at least 90% of the species present; 2) document (through collection or museum specimen and literature review) one voucher specimen for each species identified; 3) provide a GIS-referenced list of sensitive species that are federally or state listed, rare, or worthy of special consideration that occur within priority sampling locations; 4) describe park-wide distribution of federally- or state-listed, rare, or special concern species; 5) enter all species data into the National Park Service NPSpecies database; and 6) provide all deliverables as outlined in the Mojave Network Biological Inventory Study Plan. Methods included daytime and nighttime visual encounter surveys, road driving, and pitfall trapping. Survey effort was concentrated in predetermined priority sampling areas, as well as in areas with a high potential for detecting undocumented species. We recorded 37 species during our surveys, including two species new to the park. During literature review and museum specimen database searches, we recorded three additional species from DEVA, elevating the documented species list to 40 (four amphibians and 36 reptiles). Based on our surveys, as well as literature and museum specimen review, we estimate an overall inventory completeness of 92% for Death Valley and an inventory completeness of 73% for amphibians and 95% for reptiles. Key Words: Amphibians, reptiles, Death Valley National Park, Inyo County, San Bernardino County, Esmeralda County, Nye County, California, Nevada, Mojave Desert, Great Basin Desert, inventory, NPSpecies.

Open-File Report

Science in action or science inaction? Evaluating the implementation of "best available science" in hydropower relicensing

Over the next two decades, half of all hydropower projects in the USA will require relicensing by the Federal Regulatory Commission (FERC). Relicensing proceedings invoke a range of informational sources and agency regulators are tasked with using the “best available science” (BAS) to make informed decisions about hydropower operations and management. Although embraced as the standard, BAS is not well-defined. The Kennebec and Penobscot River watersheds in Maine provide an ideal opportunity for studying BAS in the relicensing process. Using citation analysis and an online survey, we identified informational sources used in relicensing decisions for dams in this system and assessed agency perceptions of BAS. Analysis of relicensing documents (n=62) demonstrates that FERC and licensee documents are highly similar in citation composition. National Oceanic and Atmospheric Administration (NOAA) documents typically cite more sources and are three times more likely to cite peer-reviewed sources than FERC and licensee documents. Survey data reveals that federal and state agency respondents (n=49) rate peer-reviewed literature highly as BAS, followed by university, agency, and expert sources while industry and community sources rate poorly. Federal respondents report using peer-reviewed/academic sources more frequently and expert sources less frequently than state respondents. Overall, the agreement between individuals with respect to the valuation of sources is low. The reported differences in information use may be linked to disparities in the access to certain sources of information, particularly peer-reviewed literature. Enhanced understanding of information use may aid in identifying pathways for better informed relicensing decisions.

Maine

Identifying key stressors driving biological impairment in freshwater streams in the Chesapeake Bay watershed, USA

Biological communities in freshwater streams are often impaired by multiple stressors (e.g., flow or water quality) originating from anthropogenic activities such as urbanization, agriculture, or energy extraction. Restoration efforts in the Chesapeake Bay watershed, USA seek to improve biological conditions in 10% of freshwater tributaries and to protect the biological integrity of existing healthy watersheds. To achieve these goals, resource managers need to better understand which stressors are most likely driving biological impairment. Our study addressed this knowledge gap through two approaches: 1) reviewing and synthesizing published multi-stressor studies, and 2) examining 303(d) listed impairments linked to biological impairment as identified by jurisdiction regulatory agencies (the states within the watershed and the District of Columbia). Results identified geomorphology (i.e., physical habitat), salinity, and toxic contaminants as important for explaining variability in benthic community metrics in the literature review. Geomorphology (i.e., physical habitat and sediment), salinity, and nutrients were the most reported stressors in the jurisdictional impairment analysis. Salinity is likely a major stressor in urban and mining settings, whereas geomorphology was commonly reported in agricultural settings. Toxic contaminants, such as pesticides, were rarely measured; more research is needed to quantify the extent of their effects in the region. Flow alteration was also highlighted as an important urban stressor in the literature review but was rarely measured in the literature or reported by jurisdictions as a cause of impairment. These results can be used to prioritize stressor monitoring by managers, and to improve stressor identification methods for identifying causes of biological impairment.

Chesapeake Bay Watershed

Predicting broad-scale occurrences of vertebrates in patchy landscapes

Spatially explicit landscape-scale models that predict species distributions, where patches of habitat are shown as having potential to be occupied or unoccupied, are increasingly common. To successfully use such data, one should understand how these predicted distributions are created and how their relative accuracies are assessed. Geographic ranges, defined upon observations (e.g., atlases), literature review, and expert review, are a primary data layer. A map of land cover is created, often from interpretation of satellite imagery or other remotely-sensed data. Species/habitat associations are defined based upon a literature review and expert review, describing associations for habitats derived from the cover map. Included as ancillary associations are how species relate to physical features, where appropriate, such as elevation and hydrography. The three layers of information (range, land cover, and associations) are merged, often using raster-based algebraic statements that exclude unused habitats or patches outside the range of a species. The accuracy of predictions for a suite of species is typically assessed with surveys by comparing the species predicted to occur in an area to the species observed. Omission (i.e., present in species lists but not predicted) and commission (i.e., predicted but not present in lists) errors are reported. Errors may be due to many sources. For example, ranges of species change, cover types may be misidentified, species/habitat associations may be incorrect or change, or species may be rare and unlikely to be seen in surveys and judged in-error even though the species may be present. An example is given of an appropriate use of broad-scale species predicted distributions, in which patterns and threats to Maine terrestrial vertebrate diversity are summarized.

Landscape Ecology

Analysis of a human-mediated microbioinvasion: The global spread of the benthic foraminifer Trochammina hadai Uchio, 1962

A non-indigenous species (NIS) of benthic foraminifera was first identified in a core collected in 1993 in San Francisco Bay, California, USA, and subsequently identified as Trochammina hadai Uchio, 1962. Archived samples and literature reviews were used to determine that the species, which is native to Asia, arrived in San Francisco Bay between the early 1960s and 1983. Through molecular analyses of specimens, archived samples and literature reviews from 1930–1983, and site surveys of harbors and estuaries along the western North American seaboard in 1994–2024, in total more than 2500 samples, we documented the presence of T. hadai at 73 locations in the USA and four in Canada. Trochammina hadai has also been recovered at nine sites in Sweden, two in France, three in Brazil, and two locations at one site in Australia. The rapid temporal and geographic spread of the NIS T. hadai in a non-native location is illustrated by a time series from 1930 to 2024 in San Francisco Bay. Between 1980 and 1986, the species' range expanded from low abundance (1.5 %) at a single site to cover nearly the entire South Bay with > 70 % abundance at some locations. By 1995 and continuing into 2010, the species expanded its range into the central and northern portions of San Francisco Bay, commonly with abundances of > 30 % and sometimes exceeding 70 %. This expansion may predate 1995, but a lack of samples makes it difficult to be more precise. Unfortunately, two Pb-210 and Cs-137-dated cores (BC01 and BC02) recovered from northern South Bay and Central Bay did not clarify this point, but additional cores may. Trochammina hadai is an infaunal opportunist that thrives in polluted locations. We surmise the species was introduced along the west coast of the USA in Puget Sound between 1902 and the 1920s, with cultivated oysters and oyster larvae and associated plant matter and residual sediment. This probably also happened in some areas of France, Sweden, and Brazil, where Japanese oysters were introduced in 1966, 1970, and 1975, respectively. After World War II, commercial shipping expanded dramatically and, with it, the release of ballast water and sediment in receiving ports, which introduced NIS worldwide. This primary vector of introduction occurred in large industrial harbors in several countries, sometimes followed by secondary introductions in small industrial centers and marinas by mud attached to the anchors and anchor chains of smaller boats.

Journal of Micropalaeontology

Distribution, abundance, and genomic diversity of the endangered antioch dunes evening primrose (Oenothera deltoides subsp. howellii) surveyed in 2019

Sand dune ecosystems are highly dynamic landforms found along coastlines and riverine deltas where a supply of sand-sized material is available to be delivered by aquatic and wind environments. These unique ecosystems provide habitat for a variety of endemic and rare plant and animal species. Sand dunes have been affected by human development, sand mining, and shoreline stabilization from invasive weeds. This report provides a summary of a comprehensive literature review, field survey, and genomic analysis for the Antioch Dunes evening primrose ( Oenothera deltoides subsp. howellii , hereafter howellii ), an endemic species to the San Francisco Bay-Delta, California, which was listed as a federally endangered subspecies in 1978. Howellii is found on a historic dune sheet (the Antioch sand sheet) near the confluence of the Sacramento and San Joaquin Rivers. The Antioch sand sheet has been greatly altered by sand mining and land conversion into agriculture and urban development. In chapter A, we describe results of the literature review and field survey. We found howellii at eight locations with over 90 percent of the adult population and nearly 99 percent of juveniles observed on the Antioch Dunes National Wildlife Refuge. We measured a negative relationship between howellii numbers and invasive weed cover, illustrating the importance of mobilized open sand for this species. In chapter B, we describe the genomic study results. We surveyed genomic diversity by using double-digest restriction-site associated sequencing to estimate population genetic structure and levels of diversity across all surveyed occurrences. The genomic analyses included outgroup samples of the closely related Oenothera deltoides subsp. cognata and three occurrences of an unknown taxon with intermediate morphology to cognata and howellii , which also occurs on the Antioch sand sheet, east of the Antioch Dunes National Wildlife Refuge. These three morphologically distinctive groups formed genetically distinctive clusters and well-supported monophyletic clades in clustering and phylogenetic analyses, respectively. There was no indication of recent hybridization among any of the groups. Among howellii occurrences, the Antioch Dunes National Wildlife Refuge contained the greatest genetic diversity. Our approach, which combined field surveys, habitat assessments, and genetic analyses, can provide useful information for the conservation and management of rare and at-risk plant species and highlights the uniqueness of the Antioch sand sheet floral diversity through the discovery of a putative new taxon within the bird-cage evening primrose species complex.

California

Risk assessment for bull trout introduction into Sullivan Lake and Harvey Creek, northeastern Washington

The Kalispel Tribe of Indians (KT), U. S. Fish and Wildlife Service, and Washington Department of Fish and Wildlife are engaged in conservation of bull trout ( Salvelinus confluentus ) in the Lake Pend Oreille (LPO) Core Area. The LPO is a complex habitat core area which falls within three states (Montana, Idaho, and Washington) and a tribal entity. As part of the conservation process, KT worked in cooperation with the U. S. Geological Survey (USGS) to complete a risk assessment for introduction of bull trout into Sullivan Lake/Harvey Creek, northeastern, Washington. The risk assessment was designed to evaluate potential risks to resident fish species, to bull trout introduced into Sullivan Lake, and to bull trout donor source populations. This risk assessment describes the potential risks associated with pathogens (introduction of pathogens and increased pathogen burden), genetics (such as risk to donor sources, straying and breeding with native bull trout, and introduction of bull-brook hybrids), and ecological interactions (such as predation and competition). Potential donor source populations were identified and evaluated using a qualitative approach based on expert opinion and a decision framework. Literature reviews were completed for fish species composition and abundance in Sullivan Lake basin to assess potential ecological interactions and risks to these populations and to the introduced bull trout. The USGS assessed pathogen risks through two major questions: (1) whether introduced bull trout might bring pathogens into the Sullivan Lake basin that were not previously present and (2) whether the health of introduced bull trout could be adversely affected by pathogens already present in the basin. Assessment of genetic risks included demographic risks to donor source populations, potential for hybridization with native bull trout, and the risk of introducing bull-brook hybrids. Literature reviews were used in conjunction with discussions among regional biologists to identify potential donor source populations and their population attributes. A decision framework was developed by USGS in collaboration with KT biologists that identified desirable population attributes (life history behavior, abundance, population viability, feasibility of collection, and environmental match) associated with donor source populations and established ranking criteria. The population attribute information was used with the (1) decision framework, (2) established ranking criteria, and (3) expert opinion of regional biologists, to assign scores for overall ranking of donor source populations. The LPO source population was the highest ranked and is considered a robust and stable population. The risk of introducing pathogens from LPO into Sullivan Lake via a bull trout introduction program seems low, and indirect pathogen burden risks to resident species can be mitigated using established pathogen surveillance methods. The likelihood that bull trout, introduced into Sullivan Lake, stray and spawn with native bull trout is low. Nearest-neighbor donor source populations, such as LPO, could minimize negative fitness impacts that might occur from straying and interbreeding of individuals that become entrained and help maintain natural patterns of genetic diversity in native populations. The ecological risk that a bull trout introduction presents to resident species seems to be low but with some uncertainty. Pygmy whitefish, a Washington State Sensitive species, is likely most vulnerable to extirpation with increased predation pressure with introduction of an additional piscivore into the ecosystem. The status of the pygmy whitefish in Sullivan Lake is unknown. The ecological risks most likely to reduce the viability of introduced bull trout are predation by burbot and an adequate forage base in Sullivan Lake. Prior fish surveys provided data on resident species abundance, provided an established baseline for effective monitoring, and identifying ecosystem changes post-bull trout introduction to inform future adaptive management decisions.

Washington

Diagnostic methodology is critical for accurately determining the prevalence of ichthyophonus infections in wild fish populations

Several different techniques have been employed to detect and identify Ichthyophonus spp. in infected fish hosts; these include macroscopic observation, microscopic examination of tissue squashes, histological evaluation, in vitro culture, and molecular techniques. Examination of the peer-reviewed literature revealed that when more than 1 diagnostic method is used, they often result in significantly different results; for example, when in vitro culture was used to identify infected trout in an experimentally exposed population, 98.7% of infected trout were detected, but when standard histology was used to confirm known infected tissues from wild salmon, it detected ~50% of low-intensity infections and ~85% of high-intensity infections. Other studies on different species reported similar differences. When we examined a possible mechanism to explain the disparity between different diagnostic techniques, we observed non-random distribution of the parasite in 3-dimensionally visualized tissue sections from infected hosts, thus providing a possible explanation for the different sensitivities of commonly used diagnostic techniques. Based on experimental evidence and a review of the peer-reviewed literature, we have concluded that in vitro culture is currently the most accurate diagnostic technique for determining infection prevalence of Ichthyophonus, particularly when the exposure history of the population is not known.

Journal of Parasitology