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Completion Summary for Well NRF-16 near the Naval Reactors Facility, Idaho National Laboratory, Idaho

In 2009, the U.S. Geological Survey in cooperation with the U.S. Department of Energy's Naval Reactors Laboratory Field Office, Idaho Branch Office cored and completed well NRF-16 for monitoring the eastern Snake River Plain (SRP) aquifer. The borehole was initially cored to a depth of 425 feet below land surface and water samples and geophysical data were collected and analyzed to determine if well NRF-16 would meet criteria requested by Naval Reactors Facility (NRF) for a new upgradient well. Final construction continued after initial water samples and geophysical data indicated that NRF-16 would produce chemical concentrations representative of upgradient aquifer water not influenced by NRF facility disposal, and that the well was capable of producing sustainable discharge for ongoing monitoring. The borehole was reamed and constructed as a Comprehensive Environmental Response Compensation and Liability Act monitoring well complete with screen and dedicated pump. Geophysical and borehole video logs were collected after coring and final completion of the monitoring well. Geophysical logs were examined in conjunction with the borehole core to identify primary flow paths for groundwater, which are believed to occur in the intervals of fractured and vesicular basalt and to describe borehole lithology in detail. Geophysical data also were examined to look for evidence of perched water and the extent of the annular seal after cement grouting the casing in place. Borehole videos were collected to confirm that no perched water was present and to examine the borehole before and after setting the screen in well NRF-16. Two consecutive single-well aquifer tests to define hydraulic characteristics for well NRF-16 were conducted in the eastern SRP aquifer. Transmissivity and hydraulic conductivity averaged from the aquifer tests were 4.8 x 103 ft2/d and 9.9 ft/d, respectively. The transmissivity for well NRF-16 was within the range of values determined from past aquifer tests in other wells near NRF of 4.4 x 102 to 5.1 x 105 ft2/d. Water samples were analyzed for metals, nutrients, total organic carbon, volatile organic compounds, semi-volatile organic compounds, herbicides, pesticides, polychlorinated biphenols, and radionuclides. All chloride, nitrate, and sulfate concentrations were less than background concentrations for the eastern SRP aquifer north of the NRF. Concentrations in water samples for most of the organic compounds and radionuclides were less than the reporting limits and reporting levels.

Scientific Investigations Report↗

Low occurrence of ranavirus in the Prairie Pothole Region of Montana and North Dakota (USA) contrasts with prior surveys

Ranaviruses are emerging pathogens that have caused mortality events in amphibians worldwide. Despite the negative effects of ranaviruses on amphibian populations, monitoring efforts are still lacking in many areas, including in the Prairie Pothole Region (PPR) of North America. Some PPR wetlands in Montana and North Dakota (USA) have been contaminated by energy-related saline wastewaters, and increased salinity has been linked to greater severity of ranavirus infections. In 2017, we tested tissues from larvae collected at 7 wetlands that ranged in salinity from 26 to 4103 mg Cl l -1 . In 2019, we used environmental DNA (eDNA) to test for ranaviruses in 30 wetlands that ranged in salinity from 26 to 11754 mg Cl l -1 . A previous study (2013-2014) found that ranavirus-infected amphibians were common across North Dakota, including in some wetlands near our study area. Overall, only 1 larva tested positive for ranavirus infection, and we did not detect ranavirus in any eDNA samples. There are several potential reasons why we found so little evidence of ranaviruses, including low larval sample sizes, mismatch between sampling and disease occurrence, larger pore size of our eDNA filters, temporal variation in outbreaks, low host abundance, or low occurrence or prevalence of ranaviruses in the wetlands we sampled. We suggest future monitoring efforts be conducted to better understand the occurrence and prevalence of ranaviruses within the PPR.

Montana, North Dakota↗

Stream salamander species richness and abundance in relation to environmental factors in Shenandoah National Park, Virginia

Stream salamanders are sensitive to acid mine drainage and may be sensitive to acidification and low acid neutralizing capacity (ANC) of a watershed. Streams in Shenandoah National Park, Virginia, are subject to episodic acidification from precipitation events. We surveyed 25 m by 2 m transects located on the stream bank adjacent to the water channel in Shenandoah National Park for salamanders using a stratified random sampling design based on elevation, aspect and bedrock geology. We investigated the relationships of four species ( Eurycea bislineata , Desmognathus fuscus , D. monticola and Gyrinophilus porphyriticus ) to habitat and water quality variables. We did not find overwhelming evidence that stream salamanders are affected by the acid-base status of streams in Shenandoah National Park. Desmognathus fuscus and D. monticola abundance was greater both in streams that had a higher potential to neutralize acidification, and in higher elevation (>700 m) streams. Neither abundance of E. bislineata nor species richness were related to any of the habitat variables. Our sampling method preferentially detected the adult age class of the study species and did not allow us to estimate population sizes. We suggest that continued monitoring of stream salamander populations in SNP will determine the effects of stream acidification on these taxa.

Virginia↗

Alternative tissue analysis method developed for organochlorine contaminants in aquatic organisms

The exposure of aquatic life to organochlorine contaminants has been investigated during the past two decades because of human and ecosystem health concerns related to the bioaccumulation of hazardous, lipophilic substances. The toxic effects of polychlorodibenzo-12-dioxins and polychlorinated biphenyls (PCBs) are well known, and recent evidence also suggests that low level exposure to lipophilic organochlorines may interfere with normal development during sensitive early life history stages (Hileman 1993). As the use of lipophilic organochlorines, such as DDT, in third world countries continues and with the purported global cycling (Bidleman and Olney 1974; Tanabe et al. 1983) and food chain accumulation (Thomann and Connoly 1984, Thomann 1989) of persistent organochlodnes, the occurrence of these compounds in aquatic organisms is a critical global environmental issue. An understanding of the fate of organochlorines in the environment clearly remains an extremely important subject related to water quality. The U.S. Geological Survey (USGS) has recently gained congressional approval in the United States to track nation wide trends in water quality through the establishment of the National Water Quality Assessment Program (NAWQA). Among the goals defined by NAWQA, aquatic organisms, including fish, shellfish, and plants, collected from major drainage basins will be analyzed for, along with other contaminants, polychlorinated biphenyls (PCBs), organochlorine insecticides, and chlorobenzenes (Crawford and Luoma 1992). The purpose of this report is to present quality assurance data obtained from the development of a PCB, chlorobenzene, and organochlorine insecticide tissue analysis method in support of NAWQA and other large-scale water quality programs conducted through our laboratory.

Bulletin of Environmental Contamination and Toxico↗

Geologic and physiographic controls on bed-material yield, transport, and channel morphology for alluvial and bedrock rivers, western Oregon

The rivers of western Oregon have diverse forms and characteristics, with channel substrates ranging from continuous alluvial gravel to bare bedrock. Analysis of several measurable morphologic attributes of 24 valley reaches on 17 rivers provides a basis for comparing nonalluvial and alluvial channels. Key differences are that alluvial reaches have greater bar area, greater migration rates, and show systematic correlation among variables relating grain size to bed-material transport capacity. We relate these differences between channel types to bed-material transport rates as derived from a coupled regional analysis of empirical sediment yield measurements and physical experiments of clast attrition during transport. This sediment supply analysis shows that overall bed-material transport rates for western Oregon are chiefly controlled by (1) lithology and basin slope, which are the key factors for bed-material supply into the stream network, and (2) lithologic control of bed-material attrition from in-transport abrasion and disintegration. This bed-material comminution strongly affects bed-material transport in the study area, reducing transport rates by 50%–90% along the length of the larger rivers in the study area. A comparison of the bed-material transport estimates with the morphologic analyses shows that alluvial gravel-bed channels have systematic and bounding relations between bed-material transport rate and attributes such as bar area and local transport capacity. By contrast, few such relations are evident for nonalluvial rivers with bedrock or mixed-bed substrates, which are apparently more influenced by local controls on channel geometry and sediment supply. At the scale of western Oregon, the physiographic and lithologic controls on the balance between bed-material supply and transport capacity exert far-reaching influence on the distribution of alluvial and nonalluvial channels and their consequently distinctive morphologies and behaviors—differences germane for understanding river response to tectonics and environmental perturbations, as well as for implementing effective restoration and monitoring strategies.

California, Nevada, Oregon, Washington↗

Implications of the Bangor Hydro decision on FERC relicensings

In the next 15 years, over 400 existing nonfederal dams will require new licenses by the Federal Energy Regulatory Commission (FERC) in order to continue operations. In many cases, state or federal agencies have a unique opportunity to condition the hydropower licenses to protect environmental values. In 1996, the Court of Appeals for the District of Columbia Circuit issued an opinion striking down a mandatory license condition imposed by the U.S. Department of the Interior in a FERC relicensing. The case, Bangor Hydro-Electric Company v.Federal Energy Regulatory Commission, is instructive for agencies regarding the procedures to be followed in imposing mandatory FERC licensing conditions in the future. After Bangor Hydro, agencies should: (1) support their decisions by substantial evidence in the FERC record, (2) request applicant studies to support agency decisions and allow public comment on the decisions as a means to test the facts and analysis, and (3) consider intervening in a case to defend the condition on appeal. Bangor Hydro also raises, but does not decide, the issue of whether the agency imposing the mandatory condition should engage in balancing the economics of the hydropower project with the public resources affected by the project.

Rivers↗

Occurrence and in vitro bioactivity of estrogen, androgen, and glucocorticoid compounds in a nationwide screen of United States stream waters

In vitro bioassays are sensitive, effect-based tools used to quantitatively screen for chemicals with nuclear receptor activity in environmental samples. We measured in vitro estrogen (ER), androgen (AR), and glucocorticoid receptor (GR) activity, along with a broad suite of chemical analytes, in streamwater from 35 well-characterized sites (3 reference and 32 impacted) across 24 states and Puerto Rico. ER agonism was the most frequently detected with nearly all sites (34/35) displaying activity (range, 0.054–116 ng E2Eq L –1 ). There was a strong linear relationship ( r 2 = 0.917) between in vitro ER activity and concentrations of steroidal estrogens after correcting for the in vitro potency of each compound. AR agonism was detected in 5/35 samples (range, 1.6–4.8 ng DHTEq L –1 ) but concentrations of androgenic compounds were largely unable to account for the in vitro activity. Similarly, GR agonism was detected in 9/35 samples (range, 6.0–43 ng DexEq L –1 ); however, none of the recognized GR-active compounds on the target-chemical analyte list were detected. The utility of in vitro assays in water quality monitoring was evident from both the quantitative agreement between ER activity and estrogen concentrations, as well as the detection of AR and GR activity for which there were limited or no corresponding target-chemical detections to explain the bioactivity. Incorporation of in vitro bioassays as complements to chemical analyses in standard water quality monitoring efforts would allow for more complete assessment of the chemical mixtures present in many surface waters.

Environmental Science & Technology↗

Germanium redistribution during weathering of Zn mine wastes: Implications for environmental mobility and recovery of a critical mineral

Germanium (Ge) is a metal used in emerging energy technologies, communications, and defense, and has been deemed critical by the United States due to its essential applications and scarce supply. Germanium is recovered as a byproduct of zinc (Zn) sulfides, and mining and processing of these materials lead to waste that could act both as a source of extractable Ge and a source for exposure to humans and ecosystems. Yet the distribution, speciation, and mineral hosts of Ge in mining-impacted areas are poorly understood. The Tar Creek Superfund Site, a former Zn mining area and Ge producer, is a natural laboratory to understand the environmental behavior and economic implications of Ge in mine wastes. We studied the distribution and behavior of Ge in solid wastes at the Tar Creek Superfund Site using bulk and microanalytical techniques. In wastes at this site we find that Ge has been redistributed from its original host, sphalerite (ZnS), to the fine-grained weathering product hemimorphite (Zn4Si2O7(OH)2·H2O), which impacts germanium's mobility, bioaccessibility, and potential for recovery. We provide chemical and mineralogical evidence of this redistribution, along with an evaluation of the oxidation state and molecular-scale substitution of Ge into sphalerite, hemimorphite, and quartz. Geochemical modeling shows that hemimorphite is more stable than sphalerite in waste piles and provides a stable secondary repository for Ge. However, hemimorphite is fine-grained, and if ingested or inhaled is readily soluble, with the potential to release Ge. Lastly, we discuss other sites internationally where similar behavior may be important. This study shows that weathering can have a significant impact on the distribution, speciation, and mineral hosts of Ge in mine wastes; directly influence mobilization from waste piles and subsequent availability to humans and ecosystems; and dictate metallurgical strategies to target Ge for recovery.

Oklahoma↗

A hierarchical modelling framework for estimating individual- and population-level reproductive success from movement data

Rapidly advancing animal telemetry technologies paired with new statistical models can provide insight into the behaviour of otherwise unobservable free-living animals. Changes in behaviour apparent from pairing telemetry with statistical models often occur as animals undertake key life-history activities, such as reproduction. For many species that are secretive or occupy remote areas, these life-history events are difficult to detect with conventional survey techniques, and consequently, vital rates are difficult to estimate. We present a hierarchical modelling framework, which integrates movement data observed via animal-borne telemetry and optional, infrequent survey data, to estimate individual- and population-level reproductive success. The approach combines a mechanistic movement model and survival model, and allows for assessing the effects of hypothesized individual and environmental covariates on reproductive success. We first tested our approach with simulated data, and then applied it to movement data from migratory golden eagles ( Aquila chrysaetos) breeding in southcentral Alaska across four breeding seasons. We show that results supported our biological hypotheses that changes in movement coincided with the timing of reproductive failures, and that changes in movement could be used to assess breeding success (and failure) at the individual and population levels. The analysis also provided evidence of inter-annual variation in population-level nest success and the timing of nesting failures. This new approach is adaptable to many species that care for young and can be tracked with telemetry devices, and can provide not only individual-level information useful for testing ecological hypotheses, but estimates of demographic parameters that can directly inform conservation and management if tagged animals are representative of the population.

Methods in Ecology and Evolution↗

Assessment of salinity retention or mobilization by sediment-retention ponds near Delta, Colorado, 2019

Salinity control efforts in the Colorado River Basin have focused on mobilization of salts from irrigated land, but nonirrigated rangelands are also a source of salinity. In particular, lands where soils have formed from the Late Cretaceous Mancos Shale under arid and semiarid climates contain considerable quantities of salt, mainly in the subsurface. Hundreds of thousands of contour furrows and check dams (gully plugs) were constructed by the Bureau of Land Management (BLM) and Bureau of Reclamation in the late 1950s and 1960s to reduce runoff, sedimentation, and salt mobilization from ephemeral stream channels on rangelands. Sediment-retention ponds associated with check dams are dry most of the year, except immediately following substantial rain events. Generally, no maintenance has been performed on these structures, some have degraded over time, and their current and past influence on salinity is poorly understood. To assess the influence of check dams and their associated ponds on salt retention and mobilization, the U.S. Geological Survey, in cooperation with the BLM, conducted a study of such ponds within the Gunnison Gorge National Conservation Area (GGNCA) near Delta, Colorado. This report includes conceptual models of how sediment-retention ponds function relative to salinity, and a collection of environmental data to evaluate the conceptual models. An inventory of 69 ponds indicated that 38 percent no longer had water holding capacity, and another 20 percent could hold 1 foot or less of water. Check-dam degradation was the main cause, but sediment infill of ponds contributed as well. Water content of soil profiles collected beneath ponds and immediately downstream from check dams indicated little penetration of water below 60 centimeters for most ponds and little evidence for lateral movement of water beneath check dams. Patterns of salt content in the soil profiles indicated no accumulation of salts at the pond surface from evaporating waters and little evidence for salt redistribution in the form of salt bulges or salt depletion curves at intermediate depths. Based on the conceptual models presented and interpretations of data collected by this study, it appears that the sediment-retention ponds in the GGNCA have neither mobilized nor retained substantial quantities of salt during their lifetimes.

Colorado↗

Hydrologic investigations and a preliminary conceptual model of the groundwater system at North Penn Area 1 Superfund Site, Souderton, Montgomery County, Pennsylvania

The U.S. Geological Survey (USGS) conducted hydrogeologic investigations, reviewed existing data, and developed a preliminary conceptual model of the groundwater system as part of technical support of the U.S. Environmental Protection Agency (EPA) at the North Penn Area 1 Superfund Site (hereafter, the NP1 Site) located within the Borough of Souderton in Montgomery County, Pennsylvania. Field work and monitoring took place during 2012–18. The area is underlain by sedimentary formations that form a fractured-rock aquifer used for drinking water and industrial supply. The EPA placed the Site on the National Priorities List in 1989, identifying tetrachloroethylene (PCE) and trichloroethylene (TCE) as contaminants of concern. During 2012–18, the USGS conducted field activities that included drilling an 82-foot (ft)-deep monitoring well (MG 2220) in 2016, reconstructing a 208-ft-deep former industrial production well (MG 668 [Granite Knitting Mill]), and collecting borehole geophysical and video logs and water levels from those and five additional wells, which ranged in depth from about 50 to 200 ft below land surface. Continuous water levels were collected during 2014–17, and a synoptic set of water levels were measured in April 2018 in the seven wells. The borehole geophysical logs (caliper, acoustic televiewer, natural gamma, single-point resistance, vertical flow, and fluid temperature and resistivity) and borehole video logs in the seven wells were evaluated to assess potential for lithologic correlation and to identify and describe water-bearing features, which included both low- and high-angle fractures and other openings oriented along dipping bedding planes, joints, or possible faults. Borehole geophysical logs collected by USGS in 1992 in a 300-ft-deep former production well near the Site were also evaluated. Few to no distinctive features were identified on geophysical logs (natural gamma and single-point resistance) that could be used for correlation, thus limiting this approach to determining local geologic structure. Extensive fracturing in the upper 62 ft of monitoring well MG 2220 indicates that the well was likely drilled through a zone of faulting, and other evidence of faulting is present in the area near the Site. Assessment of continuous water levels showed hydraulic connections among some wells as indicated by rising or falling water levels in response to changes in pumping rates at nearby wells. A map of water levels measured in April 2018 indicates potential for groundwater flow generally toward the stream to the south and southwest of the Site, but the limited water-level data are insufficient to describe vertical groundwater gradients or lateral gradients in any detail. Review of 1999–2022 volatile organic compound (VOC) monitoring data collected by the Pennsylvania Department of Environmental Protection for five monitoring wells indicates that the highest groundwater concentrations of PCE and TCE were found in samples from extraction well MG 2201 (S-1) downgradient from, and nearest to, the previously identified Site contaminant source area, and these concentrations fluctuated through time. PCE concentrations were higher than TCE concentrations in samples from all five monitoring wells and were much higher than TCE concentrations in samples from extraction well MG 2201 (S-1). Temporally variable recharge is a possible factor affecting observed fluctuations in PCE concentrations in groundwater samples from well extraction MG 2201 (S-1), as indicated by a general inverse relation between PCE concentrations and water levels in a nearby long-term observation well. The PCE concentration of 1,830 micrograms per liter (μg/L) in a May 2018 water sample from monitoring well MG 2220 was more than four times the PCE concentration of 444 μg/L in a December 2017 sample from the nearby extraction well MG 2201 (S-1), which is open to fewer fractures. Low concentrations of VOCs were measured in surface water at two stream sites downgradient from wells with the highest groundwater VOC concentrations at the Site, indicating that discharge of contaminated groundwater to the stream is likely. Development of a conceptual model of the groundwater system was constrained by limited data. In areas with no pumping, groundwater-flow directions generally are thought to be controlled by topography and geologic structure (bedding orientation) and likely to the south and southwest of the Site, with local flow directions affected by orientations of fractures, joints, and local faults. Additional investigations that could help improve the conceptual model of the groundwater system and help delineate the extent of groundwater contamination and its transport are discussed.

Pennsylvania↗

Influences of water hardness on chronic toxicity of potassium chloride to a unionid mussel (Lampsilis siliquoidea)

Elevated concentrations of potassium (K) often occur in effluents from wastewater treatment plants, oil and gas production operations, mineral extraction processes, and from other anthropogenic sources. Previous studies have demonstrated that freshwater mussels are highly sensitive to K in acute and chronic exposures, and acute toxicity of K decreases with increasing water hardness. However, little is known about the influence of hardness on the chronic toxicity of K. The objective of this study was to evaluate the chronic toxicity of K (tested as KCl) to a commonly tested unionid mussel (fatmucket , Lampsilis siliquoidea ) at five hardness levels (25, 50, 100, 200, 300 mg/L as CaCO 3 ) representing most surface waters in the United States. Chronic 28-d K toxicity tests were conducted with 3-week-old juvenile fatmucket in the five hardness waters using an ASTM standard method. The maximum acceptable toxicant concentrations (geometric mean of the no-observed-effect concentration and the lowest-observed-effect concentration) increased from 15.1 to 69.3 mg K/L for survival and from 15.1 to 35.8 mg K/L for growth (length and dry weight) and biomass when water hardness was increased from 25 mg/L (soft) to 300 mg/L (very hard). These results provided evidence to support water hardness influence on chronic K toxicity to juvenile fatmucket. However, the chronic effect concentrations based on the more sensitive endpoint (growth or biomass) increased only 2.4-fold from the soft water to the very hard water, indicating that water hardness had limited influence on the chronic toxicity of K to the mussels. These results can be used to establish chronic toxicity thresholds for K across a broad range of water hardness and to derive environmental guideline values for K to protect freshwater mussels and other organisms.

Environmental Toxicology and Chemistry↗

Population trends, bend use relative to available habitat and within-river-bend habitat use of eight indicator species of Missouri and Lower Kansas River benthic fishes: 15 years after baseline assessment

A baseline assessment of the Missouri River fish community and species-specific habitat use patterns conducted from 1996 to 1998 provided the first comprehensive analysis of Missouri River benthic fish population trends and habitat use in the Missouri and Lower Yellowstone rivers, exclusive of reservoirs, and provided the foundation for the present Pallid Sturgeon Population Assessment Program (PSPAP). Data used in such studies are frequently zero inflated. To address this issue, the zero-inflated Poisson (ZIP) model was applied. This follow-up study is based on PSPAP data collected up to 15 years later along with new understanding of how habitat characteristics among and within bends affect habitat use of fish species targeted by PSPAP, including pallid sturgeon. This work demonstrated that a large-scale, large-river, PSPAP-type monitoring program can be an effective tool for assessing population trends and habitat usage of large-river fish species. Using multiple gears, PSPAP was effective in monitoring shovelnose and pallid sturgeons, sicklefin, shoal and sturgeon chubs, sand shiner, blue sucker and sauger. For all species, the relationship between environmental variables and relative abundance differed, somewhat, among river segments suggesting the importance of the overall conditions of Upper and Middle Missouri River and Lower Missouri and Kansas rivers on the habitat usage patterns exhibited. Shoal and sicklefin chubs exhibited many similar habitat usage patterns; blue sucker and shovelnose sturgeon also shared similar responses. For pallid sturgeon, the primary focus of PSPAP, relative abundance tended to increase in Upper and Middle Missouri River paralleling stocking efforts, whereas no evidence of an increasing relative abundance was found in the Lower Missouri River despite stocking.

Kansas River, Missouri River↗

Prior knowledge-based approach for associating contaminants with biological effects: A case study in the St. Croix River basin, MN, WI, USA

Evaluating potential adverse effects of complex chemical mixtures in the environment is challenging. One way to address that challenge is through more integrated analysis of chemical monitoring and biological effects data. In the present study, water samples from five locations near two municipal wastewater treatment plants in the St. Croix River basin, on the border of MN and WI, USA, were analyzed for 127 organic contaminants. Known chemical-gene interactions were used to develop site-specific knowledge assembly models (KAMs) and formulate hypotheses concerning possible biological effects associated with chemicals detected in water samples from each location. Additionally, hepatic gene expression data were collected for fathead minnows ( Pimephales promelas ) exposed in situ , for 12 d, at each location. Expression data from oligonucleotide microarrays were analyzed to identify functional annotation terms enriched among the differentially-expressed probes. The general nature of many of the terms made hypothesis formulation on the basis of the transcriptome-level response alone difficult. However, integrated analysis of the transcriptome data in the context of the site-specific KAMs allowed for evaluation of the likelihood of specific chemicals contributing to observed biological responses. Thirteen chemicals (atrazine, carbamazepine, metformin, thiabendazole, diazepam, cholesterol, p-cresol, phenytoin, omeprazole, ethyromycin, 17β-estradiol, cimetidine, and estrone), for which there was statistically significant concordance between occurrence at a site and expected biological response as represented in the KAM, were identified. While not definitive, the approach provides a line of evidence for evaluating potential cause-effect relationships between components of a complex mixture of contaminants and biological effects data, which can inform subsequent monitoring and investigation.

Minnesota, Wisconsin↗

Geomorphology, acoustic backscatter, and processes in Santa Monica Bay from multibeam mapping

Santa Monica Bay was mapped in 1996 using a high-resolution multibeam system, providing the first substantial update of the submarine geomorphology since the initial compilation by Shepard and Emery [(1941) Geol. Soc. Amer. Spec. Paper 31]. The multibeam mapping generated not only high-resolution bathymetry, but also coregistered, calibrated acoustic backscatter at 95 kHz. The geomorphology has been subdivided into six provinces; shelf, marginal plateau, submarine canyon, basin slope, apron, and basin. The dimensions, gradients, and backscatter characteristics of each province is described and related to a combination of tectonics, climate, sea level, and sediment supply. Fluctuations of eustatic sea level have had a profound effect on the area; by periodically eroding the surface of Santa Monica plateau, extending the mouth of the Los Angeles River to various locations along the shelf break, and by connecting submarine canyons to rivers. A wetter glacial climate undoubtedly generated more sediment to the rivers that then transported the increased sediment load to the low-stand coastline and canyon heads. The trends of Santa Monica Canyon and several bathymetric highs suggest a complex tectonic stress field that has controlled the various segments. There is no geomorphic evidence to suggest Redondo Canyon is fault controlled. The San Pedro fault can be extended more than 30 km to the northwest by the alignment of a series of bathymetric highs and abrupt changes in direction of channel thalwegs.

California↗

Geogenic sources of benzene in aquifers used for public supply, California

Statistical evaluation of two large statewide data sets from the California State Water Board's Groundwater Ambient Monitoring and Assessment Program (1973 wells) and the California Department of Public Health (12417 wells) reveals that benzene occurs infrequently (1.7%) and at generally low concentrations (median detected concentration of 0.024 &mu;g/L) in groundwater used for public supply in California. When detected, benzene is more often related to geogenic (45% of detections) than anthropogenic sources (27% of detections). Similar relations are evident for the sum of 17 hydrocarbons analyzed. Benzene occurs most frequently and at the highest concentrations in old, brackish, and reducing groundwater; the detection frequency was 13.0% in groundwater with tritium <1 pCi/L, specific conductance >1600 &mu;S/cm, and anoxic conditions. This groundwater is typically deep (>180 m). Benzene occurs somewhat less frequently in recent, shallow, and reducing groundwater; the detection frequency was 2.6% in groundwater with tritium ≥1 pCi/L, depth <30 m, and anoxic conditions. Evidence for geogenic sources of benzene include: higher concentrations and detection frequencies with increasing well depth, groundwater age, and proximity to oil and gas fields; and higher salinity and lower chloride/iodide ratios in old groundwater with detections of benzene, consistent with interactions with oil-field brines.

California↗

Clinal variation or validation of a subspecies? A case study of the Graptemys nigrinoda complex (Testudines: Emydidae)

Widely distributed species often display intraspecific morphological variation due to the abiotic and biotic gradients experienced across their ranges. Historically, in many vertebrate taxa, such as birds and reptiles, these morphological differences within a species were used to delimit subspecies. Graptemys nigrinoda is an aquatic turtle species endemic to the Mobile Bay Basin. Colour pattern and morphological variability were used to describe a subspecies ( G. n. delticola ) from the lower reaches of the system, although it and the nominate subspecies also reportedly intergrade over a large portion of the range. Other researchers have suggested that these morphological differences merely reflect clinal variation. Our molecular data (mtDNA) did not support the existence of the subspecies, as the haplotypes were differentiated by only a few base pairs and one haplotype was shared between the putative subspecies. While there were significant morphological and pattern differences among putative specimens of G. n. nigrinoda, G. n. delticola and G. n. nigrinoda × delticola , these differences probably represent clinal variation as they were also related to environmental variables [i.e. cumulative drainage area and drainage (categorical)]. Specimens occupying slow-current, high-turbidity river reaches (e.g. the Tensaw River) exhibited greater relative carapace heights and more dark pigmentation, while specimens occupying fast-current, clearer rivers (e.g. the upper Alabama, Cahaba and Tallapoosa rivers) exhibited lower carapace heights and more yellow pigmentation. Given the absence of clear molecular and morphological differences that are related to drainage characteristics, we suggest that there is not sufficient evidence for the recognition of G. n. delticola as a distinct subspecies.

Alabama;Mississippi↗

Worldwide phylogenetic relationship of avian poxviruses

Poxvirus infections have been found in 230 species of wild and domestic birds worldwide in both terrestrial and marine environments. This ubiquity raises the question of how infection has been transmitted and globally dispersed. We present a comprehensive global phylogeny of 111 novel poxvirus isolates in addition to all available sequences from GenBank. Phylogenetic analysis of the Avipoxvirus genus has traditionally relied on one gene region (4b core protein). In this study we expanded the analyses to include a second locus (DNA polymerase gene), allowing for a more robust phylogenetic framework, finer genetic resolution within specific groups, and the detection of potential recombination. Our phylogenetic results reveal several major features of avipoxvirus evolution and ecology and propose an updated avipoxvirus taxonomy, including three novel subclades. The characterization of poxviruses from 57 species of birds in this study extends the current knowledge of their host range and provides the first evidence of the phylogenetic effect of genetic recombination of avipoxviruses. The repeated occurrence of avian family or order-specific grouping within certain clades (e.g., starling poxvirus, falcon poxvirus, raptor poxvirus, etc.) indicates a marked role of host adaptation, while the sharing of poxvirus species within prey-predator systems emphasizes the capacity for cross-species infection and limited host adaptation. Our study provides a broad and comprehensive phylogenetic analysis of the Avipoxvirus genus, an ecologically and environmentally important viral group, to formulate a genome sequencing strategy that will clarify avipoxvirus taxonomy.

Journal of Virology↗