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U.S. Geological Survey, National Wildlife Health Center, 2011 report of selected wildlife diseases

The National Wildlife Health Center (NWHC) was founded in 1975 to provide technical assistance in identifying, controlling, and preventing wildlife losses from diseases, conduct research to understand the impact of diseases on wildlife populations, and devise methods to more effectively manage these disease threats. The impetus behind the creation of the NWHC was, in part, the catastrophic loss of tens of thousands of waterfowl as a result of an outbreak of duck plague at the Lake Andes National Wildlife Refuge in South Dakota during January 1973. In 1996, the NWHC, along with other Department of Interior research functions, was transferred from the U.S. Fish and Wildlife Service to the U.S. Geological Survey (USGS), where we remain one of many entities that provide the independent science that forms the bases of the sound management of the Nation’s natural resources. Our mission is to provide national leadership to safeguard wildlife and ecosystem health through dynamic partnerships and exceptional science. The main campus of the NWHC is located in Madison, Wis., where we maintain biological safety level 3 (BSL–3) diagnostic and research facilities purposefully designed for work with wildlife species. The NWHC provides research and technical assistance on wildlife health issues to State, Federal, and international agencies. In addition, since 1992 we have maintained a field station in Hawaii, the Honolulu Field Station, which focuses on marine and terrestrial natural resources throughout the Pacific region. The NWHC conducts diagnostic investigations of unusual wildlife morbidity and mortality events nationwide to detect the presence of wildlife pathogens and determine the cause of death. This is also an important activity for detecting new, emerging and resurging diseases. The NWHC provides this crucial information on the presence of wildlife diseases to wildlife managers to support sound management decisions. The data and information generated also allows for further indepth analyses for determining the biological and ecological significance of disease events, detecting disease trends over time and space, as well as detecting any significant changes to how diseases manifest in the field. Moreover, this information allows us to gain insight into the significance of future wildlife disease events. The purpose of this report is to provide a sample of NWHC data that are available from our Laboratory Information Management System (LIMS). These data are presented in summary format with minimal statistical analysis and interpretation. The goal is to share these data with wildlife managers and other stakeholders, promote the use of NHWC data, and encourage the sharing of wildlife disease data to improve temporal and geographic surveillance coverage. Continued national surveillance for wildlife diseases is essential for providing early detection and warning of events that have the potential to result in harm to human health, economic losses, declines in wildlife populations, and subsequent ecological disturbances. Increased collaboration, coordination, and sharing of surveillance data will enhance this Nation’s ability to detect and respond to wildlife disease threats.

Scientific Investigations Report↗

Mitochondrial DNA variability among Lake Baikal omul Coregonus autumnalis migratorius (Georgi)

Omul, Coregonus autumnalis, are a commercially important coregonine fish from Lake Baikal, Siberia, Russia. In Lake Baikal, three morphotypes recognized by fishery experts occupy different zones in the lake: they are referred to as "littoral," "pelagic," and "benthic". Expressed character divergence was supported by whole-body morphometric analysis, but it is not known whether discrete genetic differences accompany the observed morphological variation. This study was designed to assess the genetic variation of three different omul morphotypes sampled from different locations in Lake Baikal that were segregated by morphotype in multivariate analysis. We surveyed genetic variation with restriction fragment length polymorphism analysis of specific gene loci amplified with the polymerase chain reaction. Sequence variation was localized in the mitochondrial control region. Though no discrete genetic markers were found, there is evidence of reproductive segregation by geographic location that corroborates geographic variation in morphological characters.

Advances in Limnology↗

Inventory of land use and land cover of the Puget Sound region using Landsat digital data

Landsat multispectral scanner digital data from four bands were analyzed using computers to produce land use and land cover information of the Puget Sound region, Wash., for use by agencies in that area. The data were first geographically registered to map coordinates. This registration enabled samples of known land cover types to be digitized from the maps. Samples of the same land cover were grouped together and then subdivided by cluster analysis into spectrally similar classes. Spectral categories were associated with specific land cover classes and used to determine spectral signatures for classification of the entire region. Reclustering and reclassification techniques were developed and then employed to minimize certain classification errors. The classified data were displayed in color using a film recorder. This color image was enlarged photographically to a 1:100000 scale to match new base maps of the region. Although the result resembles a conventional polygonal land use and land cover map, certain image-like qualities remain and yield additional information about the landscape.

Washington↗

Problems of the geographic and stratigraphic distribution of American Middle Eocene larger Foraminifera

Larger Foraminifera from the middle Eocene of Florida and southern Georgia are discussed, including 2 new species, and their geographic and stratigraphic distribution, not only in the U. S., but also at selected localities in the Caribbean region, is analyzed. Several species not previously reported from the U. S. are discussed and illustrated. Analysis of the larger Foraminifera suggests that the Inglis limestone can not be separated from the underlying Avon Park Limestone and represents the upper part of the Avon Park Limestone and represents the upper part of the Avon Park Limestone. The Helicostegina gyralis zone (uppermost zone) of the Oldsmar Limestone (lower Eocene) is transferred to the basal Lake City Limestone (middle Eocene).

Florida, Georgia↗

Seafloor habitat mapping of the New York Bight incorporating sidescan sonar data

The efficacy of using sidescan sonar imagery, image classification algorithms and geographic information system (GIS) techniques to characterize the seafloor bottom of the New York Bight were assessed. The resulting seafloor bottom type map was compared with fish trawl survey data to determine whether there were any discernable habitat associations. An unsupervised classification with 20 spectral classes was produced using the sidescan sonar imagery, bathymetry and secondarily derived spatial heterogeneity to characterize homogenous regions within the study area. The spectral classes, geologic interpretations of the study region, bathymetry and a bottom landform index were used to produce a seafloor bottom type map of 9 different bottom types. Examination of sediment sample data by bottom type indicated that each bottom type class had a distinct composition of sediments. Analysis of adult summer flounder, Paralichthys dentatus, and adult silver hake, Merluccius bilinearis, presence/absence data from trawl surveys did not show evidence of strong associations between the species distributions and seafloor bottom type. However, the absence of strong habitat associations may be more attributable to the coarse scale and geographic uncertainty of the trawl sampling data than conclusive evidence that no habitat associations exist for these two species. ?? 2006 Elsevier Ltd. All rights reserved.

Estuarine, Coastal and Shelf Science↗

A possible link between Balkan endemic nephropathy and the leaching of toxic organic compounds from Pliocene lignite by groundwater: Preliminary investigation

Balkan endemic nephropathy (BEN) is a fatal kidney disease that is known to occur only in clusters of villages in alluvial valleys of tributaries of the Danube River in Bulgaria, Romania, Yugoslavia, Bosnia, and Croatia. The confinement of this disease to a specific geographic area has led to speculation that an environmental factor may be involved in the etiology of BEN. Numerous environmental factors have been suggested as causative agents for producing BEN, including toxic metals in drinking water, metal deficiency in soils of BEN areas, and environmental mycotoxins to name a few. These hypotheses have either been disproved or have failed to conclusively demonstrate a connection to the etiology of BEN, or the clustering of BEN villages. In previous work, we observed a distinct geographic relationship between the distribution of Pliocene lignites in the Balkans and BEN villages. We hypothesized that the long-term consumption of well water containing toxic organic compounds derived from the leaching of nearby Pliocene lignites by groundwater was a primary factor in the etiology of BEN. In our current work, chemical analysis using 13C nuclear magnetic resonance (13CNMR) spectroscopy indicated a high degree of organic functionality in Pliocene lignite from the Balkans, and suggested that groundwater can readily leach organic matter from these coal beds. Semi-quantitative gas chromatography/mass spectroscopy analysis of solvent extracts of groundwater from shallow wells in BEN villages indicated the presence of potentially toxic aromatic compounds, such as napthalene, fluorene, phenanthrene, and pyrene at concentrations in the ppb range. Laboratory leaching of Balkan Pliocene lignites with distilled water yielded soluble organic matter (> 500 MW) containing large amounts of aromatic structures similar to the simple/discrete aromatic compounds detected in well water from BEN villages. These preliminary results are permissive of our hypothesis and suggest that further work on the possible relationship between the etiology of BEN and toxic aromatic substances leached from Pliocene lignites in well water is warranted.A distinct geographic relationship between the distribution of Pliocene lignites in the Balkans and villages where Balkan endemic nephropathy (BEN) occurs has been observed, indicating a possible link between BEN and the long-term consumption of well water containing toxic organic compounds derived from the leaching of nearby Pliocene lignites. Preliminary investigations by NMR spectroscopy, gas chromatography/mass spectroscopy and leaching experiments show a high degree of organic functionality in the Pliocene lignites, high-leachability by groundwater of organic matter from these beds, and the presence of toxic aromatic compounds.

International Journal of Coal Geology↗

Spatial analysis of geologic and hydrologic features relating to sinkhole occurrence in Jefferson County, West Virginia

In this study the influence of geologic features related to sinkhole susceptibility was analyzed and the results were mapped for the region of Jefferson County, West Virginia. A model of sinkhole density was constructed using Geographically Weighted Regression (GWR) that estimated the relations among discrete geologic or hydrologic features and sinkhole density at each sinkhole location. Nine conditioning factors on sinkhole occurrence were considered as independent variables: distance to faults, fold axes, fracture traces oriented along bedrock strike, fracture traces oriented across bedrock strike, ponds, streams, springs, quarries, and interpolated depth to groundwater. GWR model parameter estimates for each variable were evaluated for significance, and the results were mapped. The results provide visual insight into the influence of these variables on localized sinkhole density, and can be used to provide an objective means of weighting conditioning factors in models of sinkhole susceptibility or hazard risk.

West Virginia↗

Combining NLCD and MODIS to create a land cover-albedo database for the continental United States

Land surface albedo is an essential climate variable that is tightly linked to land cover, such that specific land cover classes (e.g., deciduous broadleaf forest, cropland) have characteristic albedos. Despite the normative of land-cover class specific albedos, there is considerable variability in albedo within a land cover class. The National Land Cover Database (NLCD) and the Moderate Resolution Imaging Spectroradiometer (MODIS) albedo product were combined to produce a long-term (14 years) integrated land cover-albedo database for the continental United States that can be used to examine the temporal behavior of albedo as a function of land cover. The integration identifies areas of homogeneous land cover at the nominal spatial resolution of the MODIS (MCD43A) albedo product (500 m × 500 m) from the NLCD product (30 m × 30 m), and provides an albedo data record per 500 m × 500 m pixel for 14 of the 16 NLCD land cover classes. Individual homogeneous land cover pixels have up to 605 albedo observations, and 75% of the pixels have at least 319 MODIS albedo observations (≥ 50% of the maximum possible number of observations) for the study period (2000–2013). We demonstrated the utility of the database by conducting a multivariate analysis of variance of albedo for each NLCD land cover class, showing that locational (pixel-to-pixel) and inter-annual variability were significant factors in addition to expected seasonal (intra-annual) and geographic (latitudinal) effects.

Remote Sensing of Environment↗

The frequency and distribution of recent landslides in three montane tropical regions of Puerto Rico

Landslides are common in sttep mountainous areas of Puerto Rico where mean annual rainfall and the frequency of intense storms are high. Each year, landslides cause extensive damage to property and coccasionally result in loss of life. Average population density is high, 422 people/km2, and is increasing. This increase in population density is accompanied by growing stress on the natural environment and physical infrastructure. As a result, human populations are more vulnerable to landslide hazards. The Blanco, Cibuco, and Coamo study areas range in surface area from 276 to 350 km2 and represent the climatologic, geographic, and geologic conditions that typify Puerto Rico. Maps of recent landslides developed from 1:20 000-scale aerial photographs, in combination with a computerized geographic information system, were used to evaluate the frequency and distribution of shallow landslides in these areas. Several types of landslides were documented-rainfall-triggered debris flows, shallow soil slips, and slumps were most abundant. Hillslopes in the study area that have been anthropogenically modified, exceed 12?? in gradient, and greater than 300 m in elevation, and face the east-northeast, are most prone to landsliding. A set of simplified matrices representing geographic conditions in the three study areas was developed and provides a basis for the estimation of the spatial controls on the frequency of landslides in Puerto Rico. this approach is an example of an analysis of the frequency of landslides that is computationally simple,. and therefore, may be easily transferable to other settings.

Geomorphology↗

Caribou distribution during the post-calving period in relation to infrastructure in the Prudhoe Bay oil field, Alaska

There is concern that caribou ( Rangifer tarandus ) may avoid roads and facilities (i.e., infrastructure) in the Prudhoe Bay oil field (PBOF) in northern Alaska, and that this avoidance can have negative effects on the animals. We quantified the relationship between caribou distribution and PBOF infrastructure during the post-calving period (mid-June to mid-August) with aerial surveys from 1990 to 1995. We conducted four to eight surveys per year with complete coverage of the PBOF. We identified active oil field infrastructure and used a geographic information system (GIS) to construct ten 1 km wide concentric intervals surrounding the infrastructure. We tested whether caribou distribution is related to distance from infrastructure with a chi-squared habitat utilization-availability analysis and log-linear regression. We considered bulls, calves, and total caribou of all sex/age classes separately. The habitat utilization-availability analysis indicated there was no consistent trend of attraction to or avoidance of infrastructure. Caribou frequently were more abundant than expected in the intervals close to infrastructure, and this trend was more pronounced for bulls and for total caribou of all sex/age classes than for calves. Log-linear regression (with Poisson error structure) of numbers of caribou and distance from infrastructure were also done, with and without combining data into the 1 km distance intervals. The analysis without intervals revealed no relationship between caribou distribution and distance from oil field infrastructure, or between caribou distribution and Julian date, year, or distance from the Beaufort Sea coast. The log-linear regression with caribou combined into distance intervals showed the density of bulls and total caribou of all sex/age classes declined with distance from infrastructure. Our results indicate that during the post-calving period: 1) caribou distribution is largely unrelated to distance from infrastructure; 2) caribou regularly use habitats in the PBOF; 3) caribou often occur close to infrastructure; and 4) caribou do not appear to avoid oil field infrastructure.

Alaska↗

Potentially toxic elements in wild Agassiz’s desert tortoises: Tissue concentrations and association with disease

Background: Desert tortoise ( Gopherus agassizii ) populations have continued to decline due to infectious and other diseases, predation, and habitat alteration. The potential contribution of minerals and heavy metals to tortoise health and susceptibility to disease remains uncertain. Objective: The objective of this study was to evaluate the results of elemental analysis of trace minerals and macrominerals in scute keratin, kidney, and liver tissue from ill and dying desert tortoises salvaged for necropsy between 1993 and 2000. Methods: Salvaged tortoises were categorized by age (adult, juvenile), geographic location, and primary disease based on necropsy findings. A subset of tortoises that were injured or killed by vehicular trauma or predation but with no notable pathologic abnormalities were used as controls. A panel of 21 trace minerals and 6 macrominerals was analyzed in scute keratin, kidney, and liver tissue samples by inductively-coupled plasma spectrometry and atomic absorption spectrophotometry. Results: Necropsies were done on 46 tortoises, including 9 juveniles salvaged from 5 regions in the Colorado and Mojave deserts of California. Primary diseases were cutaneous dyskeratosis (n=9), infection/ inflammation (n=8), malnutrition (n=7), mycoplasmosis (n=5), and urolithiasis (n=3); 14 tortoises were classified as controls. Concentrations of elements differed significantly by tissue, age, desert region, and disease (P < 0.05). Tortoises with cutaneous dyskeratosis had significantly higher Se concentrations, primarily in keratin and liver, than tortoises with other diseases (P < 0.001). Juveniles were more likely than adults to have high Pb, Sn, and Zn levels (P < 0.05). All tortoises had detectable levels of more than one potentially toxic heavy metal, including As, Cd, Cr, Hg, Ni, Pb, Sn and V. Conclusions: Potentially toxic elements are frequently found in tissue from tortoises in desert regions of California, with significantly higher concentrations in diseased tortoises. Metal exposure from soils, mining, historic and ongoing military activities, and other human activities could increase susceptibility to disease in desert tortoises.

California↗

Fine-scale population structure Atlantic salmon from Maine's Penobscot River drainage

We report a survey of microsatellite DNA variation in Atlantic salmon from the unimpounded lower reaches of Maine's Penobscot River. Our analysis indicates that Atlantic salmon in the Penobscot River are distinct from other populations that have little or no history of human-mediated repopulation, including two of its tributaries, Cove Brook and Kenduskeag Stream, another Maine river, the Ducktrap, and Canada's Miramichi and Gander rivers. Significant heterogeneity was detected in allele frequency among all three subpopulations sampled in the Penobscot drainage. The high resolution of the 12-locussuite was quantified using maximum likelihood assignment tests, which correctly identified the source of 90.4–96.1% of individuals from within the Penobscot drainage. Current populations are clearly isolated from each other, however we are unable to determine from the present data whether the populations in Cove Brook and Kenduskeag Stream are recently diverged from populations stocked into the Penobscot River over the last century, or are aboriginal in origin. The degree of population structure identified in the Penobscot drainage is noteworthy in light of its lengthy history of systematic restocking, the geographic proximity of the subpopulations, and the extent of the differentiation. Similar population structure on this extremely limited geographic scale could exist among Atlantic salmon runs elsewhere in Maine and throughout the species' range and should be taken into account for future management decisions.

Maine↗

Methods for estimating magnitude and frequency of floods in Arizona, developed with unregulated and rural peak-flow data through water year 2010

Flooding is among the worst natural disasters responsible for loss of life and property in Arizona, underscoring the importance of accurate estimation of flood magnitude for proper structural design and floodplain mapping. Twenty-four years of additional peak-flow data have been recorded since the last comprehensive regional flood frequency analysis conducted in Arizona. Periodically, flood frequency estimates and regional regression equations must be revised to maintain the accurate estimation of flood frequency and magnitude. Annual peak-flow data collected through water year 2010 were compiled from 448 unregulated streamflow-gaging stations, hereafter referred to as streamgages, in Arizona having a minimum of 10 years of record. Flood frequency estimates were first computed with station (or at-site) skew using the Expected Moments Algorithm with a multiple Grubbs-Beck test to identify multiple potentially influential low flows to fit a Pearson Type III distribution. Next, a multiple step Bayesian least-squares-regression approach was used to determine a new statewide regional skew of &minus;0.09. No basin characteristics analyzed were statistically significant in explaining the variation in skew and as a result, the constant model was chosen as the best regional skew model for the Arizona study area. The mean square error used in Bulletin 17B (B17B) of the Interagency Advisory Committee on Water Data is used to describe the precision of the regional skew. The constant model had a mean square error equal to 0.08, which corresponds to an effective record length of 85 years. This is a marked improvement over a previous Arizona regional skew analysis, with a reported mean square error of 0.31, for a corresponding effective record length of around 17 years. Thus the new regional model had almost five times the information content (as measured by effective record length) of that calculated in USGS Water Supply Paper 2433, published in 1997, or the value of 0.302 reported in the B17B generalized skew map. The flood frequency estimates were recalculated using a weighted skew of the station and regional skew. Station flood frequency estimates for each streamgage are presented for the 50-, 20-, 10-, 4-, 2-, 1-, 0.5-, and 0.2-percent annual exceedance probabilities. Geographical information systems were used to compute basin characteristic information for each streamgage for the purpose of developing regional equations to estimate flood statistics at ungaged basins. Five hydrologic flood regions in Arizona were defined in a multivariate regionalization process based on mean basin elevation, mean annual precipitation, and soil permeability. A regional generalized least-squares-regression analysis was used to develop five sets of equations from 344 nonredundant streamgages, corresponding to five regions, for estimating the 50-, 20-, 10-, 4-, 2-, 1-, 0.5-, and 0.2-percent annual exceedance probabilities at ungaged basins in Arizona. The regression equations developed for these five regions were based on one or more of the statistically significant explanatory variables: drainage area, mean basin elevation, and mean annual precipitation. Average standard errors of prediction for the regression regions for the five regions ranged from 27 to 122 percent and the pseudo-coefficients of determination (pseudo-R 2 ), a measure of the proportion of peak-flow variation that is explained by the basin characteristics, ranged from 68 to 98 percent. Regression equations for Central Highlands (region 4) had the lowest model error and the greatest pseudo-R 2 metrics. The equations for Colorado Plateau (region 2) regression equations generally had greater model error and lower pseudo-R 2 metrics. The improvement of regional regression equation model error and pseudo-R 2 metrics was related to higher numbers of streamgages, longer period of record, and even spatial coverage within a region. The regional regression equations were integrated into the U.S. Geological Survey&rsquo;s StreamStats program. The StreamStats program is a national map-based web application that allows the public to easily access published flood frequency and basin characteristic statistics. The interactive web application allows a user to select a point within a watershed (gaged or ungaged) and retrieve flood-frequency estimates derived from the current regional regression equations and geographic information system data within the selected basin. StreamStats provides users with an efficient and accurate means for retrieving the most up to date flood frequency and basin characteristic data. StreamStats is intended to provide consistent statistics, minimize user error, and reduce the need for large datasets and costly geographic information system software.

Arizona↗

Riverine pesticide trends in the United States: Assessing a decade of national-scale monitoring

Pesticides in freshwater systems can compromise water availability by degrading water quality, with implications for human health and aquatic life. Despite recognition of the need for national-scale monitoring and analysis, few studies have documented long-term trends in surface water pesticide contamination across the US. This study addresses that need by analyzing temporal trends and acute and chronic benchmark exceedances for aquatic life and human health from 81 river sites sampled from 2013 to 2022 using an analytical method targeting 80 pesticides. The majority (79%) of single site and pesticide combinations had too few pesticide detections to estimate trends. When detections were more frequent, increasing trends in concentration were twice as common as decreasing trends. Increasing pesticide concentrations were common in primary drainages of the Mississippi River Basin. Aquatic life benchmarks were exceeded by 19 pesticides, and exceedances were geographically widespread, with both acute and chronic aquatic life benchmark exceedances at 62% of sites. The herbicides atrazine and metolachlor and the insecticide imidacloprid were identified as the greatest threats to surface water availability based on their trends and aquatic life benchmark exceedances. These findings demonstrate the need for continued monitoring and trend analysis, driver investigation, and management strategies to protect freshwater resources.

conterminous United States↗

A 37 K SNP array for the management and conservation of Golden Eagles (Aquila chrysaetos)

We describe the development of a custom 37 K Affymetrix Axiom myDesign single nucleotide polymorphism (SNP) array for a culturally and ecologically important apex predator, the golden eagle ( Aquila chrysaetos ). Using this SNP array, we performed population genomic analysis on 154 individuals of known natal localities and detected three genetic clusters that we designated as Taiga/High Arctic, Great Basin, and Rocky Mountains/Great Plains. Each of these clusters appears to display clinal variation within these geographic regions. After determining genetic structure, we performed an assignment test of 32 individuals, five of which were siblings of individuals used in the assessment of genetic structure, three had associated telemetry data, and the remaining individuals were of unknown natal locations. Using this array, four siblings were correctly assigned to the same geographic region as their sibling and the genetic assignment of the radio telemetered birds agreed with the expected movement patterns displayed by these individuals. For the remaining individuals, we were able to assign all but five individuals to one of the three genetic clusters. Our genetic assignments illustrates the utility of this SNP array to accurately assign most individuals to predesignated geographical regions. While further compiling genetic and other data types, we can increase the power of this tool for identifying those breeding populations that may need assistance due to anthropogenic stressors that negatively impact their population viability. The use of this genetic resource will help substantiate decisions by multiple conservation groups that seek to preserve the natural population structure of the golden eagle.

Conservation Genetics↗

Lagtime relations for urban streams in Georgia

Urban flood hydrographs are needed for the design of many highway drainage structures, embankments, and entrances to detention ponds. The three components that are needed to simulate urban flood hydrographs at ungaged sites are the design flood, the dimensionless hydrograph, and lagtime. The design flood and the dimensionless hydrograph have been presented in earlier studies for urban streams in Georgia. The objective of this study was to develop equations for estimating lagtime for urban streams in Georgia. Lagtimes were computed for 329 floods at 69 urban gaging stations in 11 cities in Georgia. These data were used to compute an average lagtime for each gaging station. Multiple regression analysis was then used to define relations between lagtime and certain physical basin characteristics, of which drainage area, slope, and impervious area were found to be significant. A qualitative variable was used to account for a geographical bias in flood-frequency region 4, a small area of southwestern Georgia. Information from this report can be used to simulate a flood hydrograph using a dimensionless hydrograph, the design flood, and the lagtime obtained from regression equations for any urban site with less than a 25-square-mile drainage area in Georgia.

Georgia↗

Delineating bird populations using ring recoveries

We delineate bird populations using cluster analysis to group ringing sites based on pairwise comparisons of recoveries. Clustering provides a quantitative (but non-unique) grouping that can be used to examine the relationships of bird distributions at both local and regional geographic scales. Clustering is based on similarity matrices composed of pairwise comparisons of recovery distributions from ringing sites. We demonstrate the method using mallard (Anas platyrhynchos) ring recoveries to group ringing sites in south-central Canada, and discuss the possibilities for these analyses for non-hunted species with few recoveries.

Book chapter↗

Design and implementation of estimation-based monitoring programs for flora and fauna: A case study on the Cherokee National Forest

Science-based monitoring of biological resources is important for a greater understanding of ecological systems and for assessment of the target population using theoretic-based management approaches. When selecting variables to monitor, managers first need to carefully consider their objectives, the geographic and temporal scale at which they will operate, and the effort needed to implement the program. Generally, monitoring can be divided into two categories: index and inferential. Although index monitoring is usually easier to implement, analysis of index data requires strong assumptions about consistency in detection rates over time and space, and parameters are often biasednot accounting for detectability and spatial variation. In most cases, individuals are not always available for detection during sampling periods, and the entire area of interest cannot be sampled. Conversely, inferential monitoring is more rigorous because it is based on nearly unbiased estimators of spatial distribution. Thus, we recommend that detectability and spatial variation be considered for all monitoring programs that intend to make inferences about the target population or the area of interest. Application of these techniques is especially important for the monitoring of Threatened and Endangered (T&E) species because it is critical to determine if population size is increasing or decreasing with some level of certainty. Use of estimation-based methods and probability sampling will reduce many of the biases inherently associated with index data and provide meaningful information with respect to changes that occur in target populations. We incorporated inferential monitoring into protocols for T&E species spanning a wide range of taxa on the Cherokee National Forest in the Southern Appalachian Mountains. We review the various approaches employed for different taxa and discuss design issues, sampling strategies, data analysis, and the details of estimating detectability using site occupancy. These techniques provide a science-based approach for monitoring and can be of value to all resource managers responsible for management of T&E species.

Proceedings of the Annual Conference of the Southe↗