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Science to support aquatic animal health

Healthy aquatic ecosystems are home to a diversity of plants, invertebrates, fish and wildlife. Aquatic animal populations face unprecedented threats to their health and survival from climate change, water shortages, habitat alteration, invasive species and environmental contaminants. These environmental stressors can directly impact the prevalence and severity of disease in aquatic populations. For example, periodic fish kills in the upper Chesapeake Bay Watershed are associated with many different opportunistic pathogens that proliferate in stressed fish populations. An estimated 80 percent of endangered juvenile Puget Sound steelhead trout die within two weeks of entering the marine environment, and a role for disease in these losses is being investigated. The introduction of viral hemorrhagic septicemia virus (VHSV) into the Great Lakes—a fishery worth an estimated 7 billion dollars annually—resulted in widespread fish die-offs and virus detections in 28 different fish species. Millions of dying sea stars along the west coast of North America have led to investigations into sea star wasting disease. U.S. Geological Survey (USGS) scientists are assisting managers with these issues through ecological investigations of aquatic animal diseases, field surveillance, and research to promote the development of mitigation strategies.

Fact Sheet↗

Evaluating the negative effect of benthic egg predators on bloater recruitment in northern Lake Michigan

As the only extant deepwater cisco in Lake Michigan, bloater is currently at record low levels of abundance. Several mechanisms to regulate their recruitment have been proposed, including skewed sex ratios, predation on their larvae by adult alewife, and climatic factors during early life history stages, but none has unequivocal support. In this research, we evaluated an alternative mechanism of egg predation that was supported by an inverse relationship between bloater recruitment and biomass of slimy sculpin , which are known to be effective egg predators. To that end, we used a combination of field sampling, laboratory experiments, and modeling to estimate the proportion of bloater eggs consumed by sculpins each year between 1973 and 2008. Monthly field sampling between January through May 2009-2010 (when bloater eggs were incubating) offshore of Frankfort (Michigan), Sturgeon Bay (Wisconsin), Two Rivers (Wisconsin), and Muskegon (Michigan) provided benthivore diets for subsequent laboratory processing. Identification and enumeration of stomach contents and subsequent genetic analyses of eggs revealed that the mean proportion of bloater eggs in slimy sculpin diets (N = 1016) equaled 0.04. Bloater eggs also were consumed by deepwater sculpins (N = 699) at a slightly lower mean proportion (0.02), and only one round goby diet among 552 enumerated revealed a bloater egg. Based on the diet results, we developed daily ration models to estimate consumption for both deepwater and slimy sculpins . We conducted feeding experiments to estimate gastric evacuation (GEVAC) for water temperatures ranging 2-5 °C, similar to those observed during egg incubation. GEVAC rates equaled 0.0115/ h for slimy sculpin and 0.0147/h for deepwater sculpin , and did not vary between 2.7 and 5.1 °C for either species or between prey types ( Mysis relicta and fish eggs) for slimy sculpin . Index of fullness [(g prey/g fish weight)100%] was estimated from sculpins sampled in bottom trawls in the same seasons and years as the diets, and varied with fish size (averaging 1.93% and 1.85% for slimy and deepwater sculpins , respectively). Estimates of daily consumption ranged from 0.2-0.8% of sculpin body weight. Annual estimates of bloater egg consumption predicted higher values for deepwater sculpin than slimy sculpin between 1973 and 2005. This pattern was reversed in 2006, 2008, 2009, 2010 as slimy sculpin abundance increased while that of deepwater sculpin declined. The sum of sculpin consumption of bloater eggs exceeded 25% of bloater population egg production early (1975-1980) and late (2008-2010) in the time series. Despite the strong field pattern implicating egg predation by slimy sculpin , our consumption models failed to fully support this hypothesis. In particular, our results were unable to explain why bloater recruitment was relatively poor during 1995-2005 when the proportion of bloater eggs consumed was very low ( < 0.06). The results did, however, demonstrate that bloater recruitment was consistently poor when the proportion of eggs consumed was relatively high. In conclusion, consumption by native benthivores can be a contributing factor to poor recruitment of bloater, especially when slimy sculpin reach high levels of abundance. This result exemplifies the importance of ecosystem-based fishery management, given that the maintenance of healthy lake trout populations in the Great Lakes should control the abundance of slimy sculpin egg predators. In addition, future research will be required to fully understand the primary bottleneck to bloater recruitment in Lake Michigan so that efforts to stock and restore bloater in Lake Ontario have a greater probability of resulting in naturalized and sustainable populations.

Lake Michigan↗

Total mercury concentrations in lionfish (Pterois volitans/miles) from the Florida Keys National Marine Sanctuary, USA

Strategies to control invasive lionfish in the western Atlantic and Caribbean are likely to include harvest and consumption. Until this report, total mercury concentrations had been documented only for lionfish from Jamaica, and changes in concentrations with increasing fish size had not been evaluated. In the Florida Keys, total mercury concentrations in dorsal muscle tissue from 107 lionfish ranged from 0.03 to 0.48 ppm, with all concentrations being less than the regulatory threshold for limited consumption. Mercury concentrations did not vary consistently with standard lengths or wet weights of lionfish. In 2010, lionfish from the upper Keys had mean concentrations that were 0.03–0.04 ppm higher than lionfish from the middle Keys, but mean concentrations did not differ consistently among years and locations. Overall, total mercury concentrations in lionfish were lower than those in several predatory fishes that support commercial and recreational fisheries in Florida.

Florida↗

Modeling management scenarios and the effects of an introduced apex predator on a coastal riverine fish community

The flathead catfish Pylodictis olivaris, a carnivorous fish species native to most of the central interior basin of North America, has been introduced into at least 13 U.S. states and 1 Canadian province. Concurrent declines in abundance of native fishes have been reported in aquatic systems where flathead catfish have been introduced. To evaluate the potential impact of this invasive species on the native fish community we developed an ecosystem simulation model (including flathead catfish) based on empirical data collected from a North Carolina coastal river. The model results suggest that flathead catfish suppress native fish community biomass by 5-50% through both predatory and competitive interactions. However, our model suggests these reductions could be mitigated through sustained exploitation of flathead catfish by recreational or commercial fishers at rates equivalent to those for native flathead catfish populations (annual exploitation = 6-25%). These findings demonstrate the potential for using directed harvest of an invasive species to assist in restoring native communities. ?? Copyright by the American Fisheries Society 2007.

Transactions of the American Fisheries Society↗

Growth, condition, diet, and consumption rates of northern pike in three Arizona reservoirs

Northern pike (Esox lucius L.) introductions are controversial in the western United States due to suspected impacts they might have on established sport fisheries and potential illegal introductions. Tbree Arizona reservoirs, Parker Canyon Lake, Upper Lake Mary and Long Lake were sampled to examine the diet, consumption dynamics, and growth of northern pike. Northern pike diets varied by season and reservoir. In Parker Canyon Lake, diets were dominated by rainbow trout in winter and spring and bluegill and green sunfish in the fall. In Long Lake the northern pike ate crayfish in spring and early summer and switched to young of the year common carp in summer and fall. Black crappie, golden shiners, and crayfish were the major prey in Upper Lake Mary during spring, but they switched to stocked rainbow trout in the fall. Northern pike growth was in the high range of growth reported throughout the United States. Estimated northern pike specific consumption rate (scr) of rainbow trout (g/g/d ?? 10-6) was greatest in Upper Lake Mary (scr = 329.1 ?? 23.7 g/g/d ?? 10-6) where stocked fingerling (<120 mm total length [TL]) rainbow trout were most vulnerable to these predators, compared to larger (>280 mm TL) rainbow trout stocked in Long Lake (scr = 1.4 ?? 0.1 g/g/d ?? 10-6) and Parker Canyon Lake (scr = 287.2 ?? 15.1 g/g/d ?? 10-6) where catchable-sized rainbow trout were stocked. Managers should consider the cost-benefits of stocking fish >200 mm TL in lakes containing northern pike. ?? Copyright by the North American Lake Management Society 2008.

Lake and Reservoir Management↗

Fishing for Northern Pike in Minnesota: A comparison of anglers and dark house spearers

In order to project fishing effort and demand of individuals targeting Northern Pike Esox lucius in Minnesota, it is important to understand the catch orientations, management preferences, and site choice preferences of those individuals. Northern Pike are specifically targeted by about 35% of the approximately 1.5 million licensed anglers in Minnesota and by approximately 14,000–15,000 dark house spearers. Dark house spearing is a traditional method of harvesting fish through the ice in winter. Mail surveys were distributed to three research strata: anglers targeting Northern Pike, dark house spearing license holders spearing Northern Pike, and dark house spearing license holders angling for Northern Pike. Dark house spearers, whether spearing or angling, reported a stronger orientation toward keeping Northern Pike than did anglers. Anglers reported a stronger orientation toward catching large Northern Pike than did dark house spearers when spearing or angling. Northern Pike regulations were the most important attribute affecting site choice for respondents in all three strata. Models for all strata indicated a preference for lakes without protected slot limits. However, protected slot limits had a stronger negative influence on lake preference for dark house spearing licensees (whether spearing or angling) than for anglers.

Minnesota↗

The effect of the lamprey larvicide, 3-trifluormethyl-4-nitrophenol, on selected aquatic invertebrates

The chemical compound 3-trifluoromethyl-4-nitrophenol (TFM) is used to control the sea lamprey (Petromyzon marinus) in the upper Great Lakes. It is introduced into streams in which sea lampreys have spawned, to kill the larvae. These 'treatments' are carried out at intervals shorter than the larval phase of the sea lamprey's life cycle (about 4 to 7 years) to prevent movement of the metamorphosed parasitic lampreys into the lakes. Most of the streams which contain sea lamprey larvae also have valuable resident fish or serve as spawning and nursery areas for fish of the Great Lakes. These species must be protected from both the direct toxic effects of the control method and from indirect effects such as destruction of food supplies. Studies have shown that TFM is nontoxic to most species of fish when used at the concentrations that kill larval lampreys. Information on the effect of TFM on aquatic invertebrates is meager. Applegate et al. reported that TFM was not harmful to selected invertebrates which they included in simulated stream tests. They also stated that no harmful effects to invertebrates were observed during actual stream application. The variety of invertebrate species used in simulated stream tests was limited, and close observation of invertebrates under stream conditions is difficult. Therefore, the present laboratory bioassays were conducted to determine the toxicity of TFM to representatives of a number of groups of invertebrates.

Transactions of the American Fisheries Society↗

Age and growth of the whitefish Coregonus clupeaformis, of Munising Bay, Lake Superior

This study is based on a sample of 415 whitefish collected in 1953 from an unexploited population in Munising Bay, Lake Superior. Gill-net and trawl catches had different length-frequency distributions and age compositions, but estimates of growth from the two catches were very similar. The body-scale relation is a straight line with an intercept of 1.5 inches. Weight of Munising Bay whitefish captured in June increased as the 3.17 power of the length. Growth in length and weight of Munising Bay whitefish was the slowest yet reported from Great Lakes waters. Munising Bay whitefish required nearly 8 years to reach a length of 10 inches and a full 13 years to reach 15 inches. Slightly more than 8 years were required to reach a weight of 8 ounces and a little less than 14 years to reach 1 pound. The sex ratio of the Munising Bay whitefish exhibited no systematic change with an increase in age. The entire sample for which ages were determined contained 52 percent males. Munising Bay whitefish matured at much shorter lengths but at a greater age than reported for other Great Lakes whitefish populations. The smallest mature males were in the 11.5- to 11.9-inch length interval (age-group VII) and all males longer than 14.4 inches (age-group XII) were mature. The first mature females appeared at 12.0-12.4 inches (age-group X) and all females longer than 14.9 inches (age-group XII) were mature. The more rapidly growing individuals matured earlier than the slow-growing ones.

Transactions of the American Fisheries Society↗

West Virginia and Landsat

Many may recall “Take Me Home, Country Roads,” made famous by John Denver, leads with the lyric “almost heaven, West Virginia, Blue Ridge Mountains, Shenandoah River.” The descriptors are apt. Nicknamed the “Mountain State,” West Virginia inspires thoughts of coal mining or logging in the Appalachian Mountains and valleys, or the leaping trout in the winding waters of New River Gorge National Park & Reserve . West Virginia is second only to Wyoming in coal production nationwide. Its mines produced more than 67 million tons of coal in 2020. Logging pumped about $3.4 billion into the State’s economy in 2019; tourist spending added another $4.6 billion. Those industries are key for West Virginia, but agriculture and fisheries also play a role in the State's economic fortunes. Peaches and apples are major drivers of food production, as are beef and poultry. Trout, meanwhile, are caught and sold commercially, and are stocked throughout the State's rivers for local and visiting anglers. Scientists, land managers, and others use imagery from the U.S. Geological Survey Landsat satellite program's deep historical archive to better understand and manage West Virginia’s storied forests, fields, mountains, and foothills.

West Virginia↗

Translating climate change effects on species into everyday language: an example of more driving and less fishing

Climate change is expected to result in widespread changes in species distributions (e.g., shifting, shrinking, expanding species ranges; e.g., Parmesan and Yohe, 2003), especially for freshwater fish species (Heino et al. 2009). Although anglers and other resource users could be greatly affected by changes in species distributions, predicted changes are rarely reported in ways that can be easily understood by the general public. In contrast, climate science that more directly affects human welfare or livelihoods is often more readily communicated to the general public because it is of greater concern or closely related to everyday life. For example, most people can readily interpret how increases in the number of &ldquo;hot&rdquo; days above a given temperature threshold might affect their lives, and property owners in coastal areas can use predictive maps to determine how they might be affected by sea level rise (for more examples, see the Third National Assessment of the U.S. Environmental Protection Agency at globalchange.gov ). However, the effects of climate change on species are usually reported to the general public using summary metrics or maps designed to communicate concepts that are not normally encountered in everyday life, including changes in habitat suitability, range shifts, or increasing risks from disease or extreme events (e.g., National Audubon Society 2009; Groffman et al. 2014). Though these metrics are necessary, meaningful, and understood by scientists, many people lack the necessary training and background to readily understand them. Further, scientists and nonscientists alike may struggle to convert these metrics into a currency that directly affects day-to-day life. Climate science is a complex issue, and we argue that when communicating potential responses of vegetation, fish, and wildlife to nonscientists, creative thinking with respect to the currency of communication will facilitate discussions between scientists, policy makers, and the public. We posit that with some additional thought and relatively simple summaries, the responses of fish and other species to climate change can be translated into everyday language that will facilitate climate science communication. Although such translations are rare, one example of this type of creativity is the translation from changes in habitat suitability for tree species to potential reductions in maple syrup production (West over 2012), which is arguably more interesting and understandable for the general public. Similar translations could be especially important for communicating climate change effects on game fish and other species that are socially and economically important to large groups of people. We demonstrate this translation by communicating the potential effects of climate change on the distribution of a coldwater fish species, the eastern Brook Trout Salvelinus fontinalis . Rather than communicating the potential forecasted contraction of the Brook Trout's distribution in terms of habitat loss, we report the predicted increases in the driving distance to streams likely offering Brook Trout angling opportunities under a climate change scenario. Travel costs based on distance have been widely used to value ecosystem services such as angling under climate change scenarios (e.g., Pendleton and Mendelsohn 1998; Mendelsohn and Markowski 1999; Ahn et al. 2000) but, to the best of our knowledge, have not been used for communicating potential changes to the public despite the intrinsic link to everyday life.

New York, Pennsylvania↗

Preliminary study of gill NA+,K+-ATPase activity in juvenile spring chinook salmon following electroshock or handling stress

We compared gill Na+,K+-ATPase in subyearling and yearling spring chinook salmon Oncorhynchus tshawytscha 3 h, 24 h, and 7 d after exposure to either a short pulsed DC electroshock (300 V, 50 Hz, 8-ms pulse duration) or an acute handling stress. Mean gill Na+,K+-ATPase values ranged from 7.5 to 11.8 ??mol inorganic phosphate (Pi) ?? (mg protein)-1 ?? h-1. No significant differences were detected, with the exception of electroshocked subyearlings 7 d after treatment. Increased activity was attributed to the presence of two influential values. No significant differences were detected after removal of these observations, so the increase was not considered biologically significant. Inclusion of the outliers did not alter our interpretation of the results given that the observed increase was slight compared with the magnitude of changes reported under experimental conditions and in migrating juvenile salmonids. The treatment groups underwent a typical stress response and had significantly elevated cortisol and glucose levels 3 h after treatment. Recovery to control levels occurred within 24 h for cortisol and from 24 h to 7 d for glucose. Our results lead to the conclusion that neither acute electroshock nor acute handling stress alters Na+,K+-ATPase activity in juvenile spring chinook salmon.

North American Journal of Fisheries Management↗

The commercial fishery in Lake Oahe, North and South Dakota, 1964-70

Ten species were commercially harvested in Lake Oahe, among which bigmouth buffalo (Ictiobus cyprinellus) predominated (63.8% of the total weight), smallmouth buffalo (Ictiobus bubalus) and goldeye (Hiodon alosoides) ranked second, and third. Variations in the seasonal and annual production of buffalo were governed by market conditions and availability. Production of minor species depended on the amount of fishing effort directed toward the harvest of buffalo. Gill and hoop nets were the principal fishing gears. The fishing season usually extended from April through December; most fishing was in May through August.

Technical Paper↗

Non-native fish control below Glen Canyon Dam - Report from a structured decision-making project

This report describes the results of a structured decision-making project by the U.S. Geological Survey to provide substantive input to the Bureau of Reclamation (Reclamation) for use in the preparation of an Environmental Assessment concerning control of non-native fish below Glen Canyon Dam. A forum was created to allow the diverse cooperating agencies and Tribes to discuss, expand, and articulate their respective values; to develop and evaluate a broad set of potential control alternatives using the best available science; and to define individual preferences of each group on how to manage the inherent trade-offs in this non-native fish control problem. This project consisted of two face-to-face workshops, held in Mesa, Arizona, October 18-20 and November 8-10, 2010. At the first workshop, a diverse set of objectives was discussed, which represented the range of concerns of those agencies and Tribes present. A set of non-native fish control alternatives ('hybrid portfolios') was also developed. Over the 2-week period between the two workshops, four assessment teams worked to evaluate the control alternatives against the array of objectives. At the second workshop, the results of the assessment teams were presented. Multi-criteria decision analysis methods were used to examine the trade-offs inherent in the problem, and allowed the participating agencies and Tribes to express their individual judgments about how those trade-offs should best be managed in Reclamation`s selection of a preferred alternative. A broad array of objectives was identified and defined, and an effort was made to understand how these objectives are likely to be achieved by a variety of strategies. In general, the objectives reflected desired future conditions over 30 years. A rich set of alternative approaches was developed, and the complex structure of those alternatives was documented. Multi-criteria decision analysis methods allowed the evaluation of those alternatives against the array of objectives, with the values of individual agencies and tribes deliberately preserved. Trout removal strategies aimed at the Paria to Badger Rapid reach (PBR), with a variety of permutations in deference to cultural values, and with backup removal at the Little Colorado River reach (LCR) if necessary, were identified as top-ranking portfolios for all agencies and Tribes. These PBR/LCR removal portfolios outperformed LCR-only removal portfolios, for cultural reasons and for effectiveness - the probability of keeping the humpback chub population above a desired threshold was estimated to be higher under the PBR/LCR portfolios than the LCR-only portfolios. The PBR/LCR removal portfolios also outperformed portfolios based on flow manipulations, primarily because of the effect of sport fishery and wilderness recreation objectives, as well as cultural objectives. The preference for the PBR/LCR removal portfolios was quite robust to variation in the objective weights and to uncertainty about the underlying dynamics, at least over the ranges of uncertainty investigated. Examination of the effect of uncertainty on the recommended outcomes allowed us to complete a 'value of information' analysis. The results of this analysis led to an adaptive strategy that includes three possible long-term management actions (no action; LCR removal; or PBR removal) and seeks to reduce uncertainty about the following two issues: the degree to which rainbow trout limit chub populations, and the effectiveness of PBR removal to reduce trout emigration downstream into Marble and eastern Grand Canyons, where the largest population of humpback chub exist. In the face of uncertainty about the effectiveness of PBR removal, a case might be made for including flow manipulations in an adaptive strategy, but formal analysis of this case was not conducted. The full set of conclusions described above is not definitive, however. This analysis described in this report is a simplified depiction of the t

Open-File Report↗

Establishment of white perch, Morone americana , in Lake Erie

White perch, Morone americana, was first reported in Lake Erie in 1953. No further reports of capture were recorded until 1973, when one fish was taken. Three other captures were confirmed in 1974, and 34 in 1975. All but 3 of the 38 specimens were taken in the warm, shallow western basin and the extreme eastern end of the central basin. The widespread distribution and the sizable numbers of specimens collected in 1975 strongly indicate that this nonendemic species has become established in Lake Erie.

Journal of the Fisheries Research Board of Canada↗

Final Project Memorandum: Ecological implications of mangrove forest migration in the southeastern U.S.

Winter climate change has the potential to have a large impact on coastal wetlands in the southeastern United States. Warmer winter temperatures and reductions in the intensity of freeze events would likely lead to mangrove forest range expansion and salt marsh displacement in parts of the U.S. Gulf of Mexico and Atlantic coast. The objective of this research was to better evaluate the ecological implications of mangrove forest migration and salt marsh displacement. The potential ecological impacts of mangrove migration are diverse ranging from important biotic impacts (e.g., coastal fisheries, land bird migration; colonial-nesting wading birds) to ecosystem stability (e.g., response to sea level rise and drought; habitat loss; coastal protection) to biogeochemical processes (e.g., carbon storage; water quality). This research specifically investigated the impact of mangrove forest migration on coastal wetland soil processes and the consequent implications for coastal wetland responses to sea level rise and carbon storage.

Report↗

Exploring life history characteristics of naturalized versus stocked chinook

Naturalization of stocked populations can result in divergence of life-history traits from domestic stocks. Lake Michigan supports popular Chinook (Oncorhynchus tshawytscha) Salmon fisheries that have been sustained by stocking since the late 1960s. Natural recruitment of Chinook Salmon in Lake Michigan has increased in the last few decades and currently contributes over 50% of Chinook Salmon recruits. Samples collected as part of a lakewide mass-marking of Lake Michigan Chinook Salmon, starting with the 2006 year class, indicated hatchery fish average 30-mm longer and 130 grams heavier than naturalized fish at age-1. We hypothesized that selective forces differ for naturalized and hatchery populations resulting in divergent life-history characteristics with implications for Chinook Salmon population production and the Lake Michigan fishery. Specific life-history metrics of interest include: age- and size- at maturity, spawning run timing, fecundity, and sex ratio. Objectives: We evaluated life history characteristics between naturally recruited and stocked Chinook Salmon in Lake Michigan to help discern potential changes resulting from naturalization and implications for fisheries. A. Conduct an analysis of historical data to determine if life-history parameters changed through time as the Chinook Salmon population became increasingly naturalized. B. Conduct a two-year field study of naturalized and hatchery stocked Chinook Salmon spawning populations to quantify differences in life-history metrics of adults. C. Determine if reproductive potential differs between naturalized and hatchery stocked Chinook salmon by measuring egg thiamine levels.

Michigan↗

Response of fish assemblages to decreasing acid deposition in Adirondack Mountain lakes

The CAA and other federal regulations have clearly reduced emissions of NOx and SOx, acidic deposition, and the acidity and toxicity of waters in the ALTM lakes, but these changes have not triggered widespread recovery of brook trout populations or fish communities. The lack of detectable biological recovery appears to result from relatively recent chemical recovery and an insufficient period for species populations to take advantage of improved water quality. Recovery of extirpated species’ populations may simply require more time for individuals to migrate to and repopulate formerly occupied lakes. Supplemental stocking of selected species may be required in some lakes with no remnant (or nearby) populations or with physical barriers between the recovered lake and source populations. The lack of detectable biological recovery could also be related to our inability to calculate measures of uncertainty or error and, thus, examine temporal changes or differences in populations and community metrics in more depth (e.g., within individual lakes) using existing datasets. Indeed, recovery of brook trout populations and partial recovery of fish communities are documented in several lakes of the region, both with and without human intervention. Multiple fish surveys (annually or within the same year) or the use of mark and recapture methods within individual lakes would help alleviate the issue (provide measures of error for key fishery metrics) within the context of a more focused sampling strategy. Efforts to evaluate and detect recovery in fish assemblages from streams may be more effective than in lakes because various life stages, species’ populations, and entire assemblages are easier to quantify, with known levels of error, in streams than in lakes. Such long-term monitoring efforts could increase our ability to detect and quantify biological recovery in recovering (neutralizing) surface waters throughout the Adirondack Region.

New York↗

Pacific lamprey artificial propogation and rearing investigations: Rocky Reach Lamprey Management Plan

The impetus for developing this document is through implementing the Rocky Reach Pacific Lamprey Management Plan (PLMP), a component of the Rocky Reach Comprehensive Settlement Agreement, both of which are discussed more thoroughly in Section 1.2. The ultimate goal of the PLMP is to achieve No Net Impact (NNI) to Pacific lamprey of ongoing operations of the Rocky Reach Hydroelectric Project. Conducting artificial propagation of Pacific lamprey was considered by the state and federal fishery agencies and Tribes that are parties to the Settlement Agreement as a potential Protection, Mitigation, and Enhancement measure (PME) for achieving NNI during the term of the current Rocky Reach license. This document is intended to provide guidance as to the feas ibility of culturing Pacific lamprey, the associated facilities necessary for culture practices, and identifying uncertainties for monitoring culture efficacy and rationale for implementing Pacific lamprey artificial propagation

Washington↗