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At least 649 records · Page 36Linked to original sources

Genetic variation in bacterial kidney disease (BKD) susceptibility in Lake Michigan Chinook Salmon and its progenitor population from the Puget Sound

Mass mortality events in wild fish due to infectious diseases are troubling, especially given the potential for long-term, population-level consequences. Evolutionary theory predicts that populations with sufficient genetic variation will adapt in response to pathogen pressure. Chinook Salmon Oncorhynchus tshawytscha were introduced into Lake Michigan in the late 1960s from a Washington State hatchery population. In the late 1980s, collapse of the forage base and nutritional stress in Lake Michigan were thought to contribute to die-offs of Chinook Salmon due to bacterial kidney disease (BKD). Previously, we demonstrated that Lake Michigan Chinook Salmon from a Wisconsin hatchery have greater survival following BKD challenge relative to their progenitor population. Here, we evaluated whether the phenotypic divergence of these populations in BKD susceptibility was due to selection rather than genetic drift. Comparison of the overall magnitude of quantitative trait to neutral marker divergence between the populations suggested selection had occurred but a direct test of quantitative trait divergence was not significant, preventing the rejection of the null hypothesis of differentiation through genetic drift. Estimates of phenotypic variation (V P ), additive genetic variation (V A ) and narrow-sense heritability (h 2 ) were consistently higher in the Wisconsin relative to the Washington population. If selection had acted on the Wisconsin population there was no evidence of a concomitant loss of genetic variation in BKD susceptibility. The Renibacterium salmoninarum exposures were conducted at both 14°C and 9°C; the warmer temperature accelerated time to death in both populations and there was no evidence of phenotypic plasticity or a genotype-by-environment (G × E) interaction. High h 2 estimates for BKD susceptibility in the Wisconsin population, combined with a lack of phenotypic plasticity, predicts that future adaptive gains in BKD resistance are still possible and that these adaptive gains would be stable under the temperature range evaluated here.

Washington;Wisconsin↗

The geology of asbestos in the United States and its practical applications

Recently, naturally occurring asbestos (NOA) has drawn the attention of numerous health and regulatory agencies and citizen groups. NOA can be released airborne by (1) the disturbance of asbestos-bearing bedrocks through human activities or natural weathering, and (2) the mining and milling of some mineral deposits in which asbestos occurs as an accessory mineral(s). Because asbestos forms in specific rock types and geologic conditions, this information can be used to focus on areas with the potential to contain asbestos, rather than devoting effort to areas with minimal NOA potential. All asbestos minerals contain magnesium, silica, and water as essential constituents, and some also contain major iron and/or calcium. Predictably, the geologic environments that host asbestos are enriched in these components. Most asbestos deposits form by metasomatic replacement of magnesium-rich rocks. Asbestos-forming environments typically display shear or evidence for a significant influx of silica-rich hydrothermal fluids. Asbestos-forming processes can be driven by regional metamorphism, contact metamorphism, or magmatic hydrothermal systems. Thus, asbestos deposits of all sizes and styles are typically hosted by magnesium-rich rocks (often also iron-rich) that were altered by a metamorphic or magmatic process. Rock types known to host asbestos include serpentinites, altered ultramafic and some mafic rocks, dolomitic marbles and metamorphosed dolostones, metamorphosed iron formations, and alkalic intrusions and carbonatites. Other rock types appear unlikely to contain asbestos. These geologic insights can be used by the mining industry, regulators, land managers, and others to focus attention on the critical locales most likely to contain asbestos.

Environmental & Engineering Geoscience↗

Occurrence of organic wastewater compounds in selected surface-water supplies, Triangle area of North Carolina, 2002-2005

Selected organic wastewater compounds, such as household, industrial, and agricultural-use compounds, sterols, pharmaceuticals, and antibiotics, were measured at eight sites classified as drinking-water supplies in the Triangle Area of North Carolina. From October 2002 through July 2005, seven of the sites were sampled twice, and one site was sampled 28 times, for a total of 42 sets of environmental samples. Samples were analyzed for as many as 126 compounds using three laboratory analytical methods. These methods were developed by the U.S. Geological Survey to detect low levels (generally less than or equal to 1.0 microgram per liter) of the target compounds in filtered water. Because analyses were conducted on filtered samples, the results presented in this report may not reflect the total concentration of organic wastewater compounds in the waters that were sampled. Various quality-control samples were used to quality assure the results in terms of method performance and possible laboratory or field contamination. Of the 108 organic wastewater compounds that met method performance criteria, 24 were detected in at least one sample during the study. These 24 compounds included 3 pharmaceutical compounds, 6 fire retardants and plasticizers, 3 antibiotics, 3 pesticides, 6 fragrances and flavorants, 1 disinfectant, and 2 miscellaneous-use compounds, all of which likely originated from a variety of domestic, industrial, and agricultural sources. The 10 most frequently detected compounds included acetyl-hexamethyl tetrahydronaphthalene and hexahydro-hexamethyl cyclopentabenzopyran (synthetic musks that are widely used in personal-care products and are known endocrine disruptors); tri(2-chloroethyl) phosphate, tri(dichloroisopropyl) phosphate, and tributyl phosphate (fire retardants); metolachlor (herbicide); caffeine (nonprescription stimulant); cotinine (metabolite of nicotine); acetaminophen (nonprescription analgesic); and sulfamethoxazole (prescription antibiotic). The occurrence and distribution of organic wastewater compounds varied considerably among sampling sites, but at least one compound was detected at every location. The most organic wastewater compounds (19) were detected at the Neuse River above U.S. 70 at Smithfield, where two-thirds of the total number of samples were collected. The fewest organic wastewater compounds (1) were detected at the Eno River at Hillsborough. The detection of multiple organic wastewater compounds was common, with a median of 3.5 and as many as 12 compounds observed in individual samples. Some compounds, including acetaminophen, cotinine, tri(2-chloroethyl) phosphate, and metolachlor, were detected at numerous sites and in numerous samples, indicating that they are widely distributed in the environment. Other organic wastewater compounds, including acetyl-hexamethyl tetrahydronaphthalene and hexahydro-hexamethyl cyclopentabenzopyran, were detected in numerous samples but at only one location, indicating that sources of these compounds are more site specific. Results indicate that municipal wastewater may be a source of antibiotics and synthetic musks; however, the three sites in this study that are located downstream from wastewater discharges also receive runoff from agricultural, urban, and rural residential lands. Source identification was not an objective of this study. Concentrations of individual compounds generally were less than 0.5 microgram per liter. No concentrations exceeded Federal drinking-water standards or health advisories, nor water-quality criteria established by the State of North Carolina; however, such criteria are available for only a few of the compounds that were studied. Compared with other surface waters that have been sampled across the United States, the Triangle Area water-supply sites had fewer detections of organic wastewater compounds; however, differences in study design and analytical methods used among studies must be considered when mak

North Carolina↗

Tritium and plutonium in waters from the Bering and Chukchi Seas

During the summer of 1993, seawater in the Bering and Chukchi Seas was sampled on a joint Russian-American cruise [BERPAC] of the RV Okean to determine concentrations of tritium, 239Pu and 240Pu. Concentrations of tritium were determined by electrolytic enrichment and liquid scintilation counting. Tritium levels ranged up to 420 mBq L-1 showed no evidence of inputs other than those attribute atmospheric nuclear weapons testing. Plutonium was recovered from water samples by ferric hydroxide precipitation, and concentrations were determined by thermal ionization mass spectrometry. 239+240Pu concentrations ranged from <1 to 5.5 [mu]Bq L-1. These concentrations are lower than those measured in water samples from other parts of the ocean during the mid-1960's to the late 1980's. The 240Pu:239Pu ratios, although associated with large uncertainties, suggest that most of the plutonium is derived from world-wide fallout. As points of comparison, the highest concentrations of tritium and plutonium observed here were about five orders of magnitude lower than the maximum permissible concentrations allowed in water released to the off-site environs from licensed nuclear facilities in the United States. This study and others sponsored by the International Atomic Energy Agency and the Office of Naval Research's Arctic Nuclear Waste Assessment Program are providing data for the assessment of potential radiological impacts in the Arctic regions associated with nuclear waste disposal by the former Soviet Union.

Health Physics↗

Arsenic in ground water: Geochemistry and occurrence

Interest in arsenic in ground water has greatly increased in the past decade because of the increased awareness of human health effects and the costs of avoidance or treatment of ground water supplies used for consumption. The goal of this book is to provide a description of the basic processes that affect arsenic occurrence and transport by providing sufficient background information on arsenic geochemistry and descriptions of hi- arsenic ground water, both affected and unaffected by human activity. An understanding of thermodynamics, adsorption, and the speciation of arsenic in solid phases, which are described in first three chapters, is needed to predict the fate of arsenic in ground water systems. Large-scale and deep movement of ground water can and has redistributed arsenic in the near surface environment, as described in the next two chapters. These large-scale systems can affect large volumes of both ground water and surface water, such as in the Yellowstone system, and can produce mineralised zones that subsequently release arsenic to ground water supplies. Regional identification of high-arsenic ground water and its consumption as described in the next three chapters clearly demonstrates a need for increased wat- quality monitoring, particularly in south and southeast Asia. Chapters 9-11 provide examples of high arsenic ground water associated with sulfide mineral oxidation and alkaline conditions. Finally, smaller scale studies of the effects of human activities that have produced high-arsenic ground water and methods for attenuation of ground water are presented

Book↗

Perspectives: Gene Expression in Fisheries Management

Functional genes and gene expression have been connected to physiological traits linked to effective production and broodstock selection in aquaculture, selective implications of commercial fish harvest, and adaptive changes reflected in non-commercial fish populations subject to human disturbance and climate change. Gene mapping using single nucleotide polymorphisms (SNPs) to identify functional genes, gene expression (analogue microarrays and real-time PCR), and digital sequencing technologies looking at RNA transcripts present new concepts and opportunities in support of effective and sustainable fisheries. Genomic tools have been rapidly growing in aquaculture research addressing aspects of fish health, toxicology, and early development. Genomic technologies linking effects in functional genes involved in growth, maturation and life history development have been tied to selection resulting from harvest practices. Incorporating new and ever-increasing knowledge of fish genomes is opening a different perspective on local adaptation that will prove invaluable in wild fish conservation and management. Conservation of fish stocks is rapidly incorporating research on critical adaptive responses directed at the effects of human disturbance and climate change through gene expression studies. Genomic studies of fish populations can be generally grouped into three broad categories: 1) evolutionary genomics and biodiversity; 2) adaptive physiological responses to a changing environment; and 3) adaptive behavioral genomics and life history diversity. We review current genomic research in fisheries focusing on those that use microarrays to explore differences in gene expression among phenotypes and within or across populations, information that is critically important to the conservation of fish and their relationship to humans.

Current Zoology↗

Analysis of body condition indices reveals different ecotypes of the Antillean manatee

Assessing the body condition of wild animals is necessary to monitor the health of the population and is critical to defining a framework for conservation actions. Body condition indices (BCIs) are a non-invasive and relatively simple means to assess the health of individual animals, useful for addressing a wide variety of ecological, behavioral, and management questions. The Antillean manatee ( Trichechus manatus manatus ) is an endangered subspecies of the West Indian manatee, facing a wide variety of threats from mostly human-related origins. Our objective was to define specific BCIs for the subspecies that, coupled with additional health, genetic and demographic information, can be valuable to guide management decisions. Biometric measurements of 380 wild Antillean manatees captured in seven different locations within their range of distribution were obtained. From this information, we developed three BCIs (BCI 1 = UG/SL, BCI 2 = W/SL 3 , BCI 3 = W/(SL*UG 2 )). Linear models and two-way ANCOVA tests showed significant differences of the BCIs among sexes and locations. Although our three BCIs are suitable for Antillean manatees, BCI 1 is more practical as it does not require information about weight, which can be a metric logistically difficult to collect under particular circumstances. BCI 1 was significantly different among environments, revealing that the phenotypic plasticity of the subspecies have originated at least two ecotypes—coastal marine and riverine—of Antillean manatees.

Puerto Rico↗

Target and suspect per- and polyfluoroalkyl substances in fish from an AFFF-impacted waterway

A major source of toxic per - and polyfluoroalkyl substances (PFAS) is aqueous film-forming foams (AFFF) used in firefighting and training at airports and military installations, however, PFAS have many additional sources in consumer products and industrial processes. A field study was conducted on fish tissues from three reaches of the Columbia Slough, located near Portland International Airport, OR, that are affected by AFFF and other PFAS sources. Fishes including largescale sucker ( Catostomus macrocheilus ), goldfish ( Carassius auratus ), and largemouth bass ( Micropterus salmoides ) were collected in 2019 and 2020. Fish blood, liver, and fillet (muscle) were analyzed for target and suspect PFAS by liquid chromatography high resolution mass spectrometry (LC-HRMS). Data were analyzed for patterns by fish species, tissue type, and river reach. Thirty-three out of 50 target PFAS and additional suspect compounds were detected at least once during the study, at concentrations up to 856 ng/g. Seven carboxylic acids (PFOA, PFNA, PFDA, PFUdA, PFDoA, PFTrDA, PFTeDA), three sulfonates (PFHxS, PFOS, PFDS), three electrofluorination-based compounds (FBSA, FHxSA, FOSA), and two fluorotelomer-based compounds (8:2 FTS, 10:2 FTS) were the most frequently detected compounds in all tissue types. The C6 (PFHxS) to C10 (PFDS) homologs were detected with PFOS and FHxSA at concentrations 1–3 orders of magnitude greater than the other PFAS detected. This is the first report of Cl-PFOS, FPeSA, and FHpSA detected in fish tissue. In all fish samples, fillet concentrations of PFAS were the lowest, followed by liver, and blood concentrations of PFAS were the highest. Differences in PFAS concentrations were driven primarily by tissue types and to a lesser extent fish species, but weakly by river reach. The Oregon Health Authority modified an existing fish consumption advisory on the Columbia Slough to recommend no whole-body consumption of most fish to avoid elevated levels of PFOS in fish liver. Measured PFAS concentrations in fish tissues indicate the potential for adverse ecological effects.

Oregon↗

Mercury burdens in Chinese mitten crabs (Eriocheir sinensis) in three tributaries of southern San Francisco Bay, California, USA

Chinese mitten crabs (Eriocheir sinensis), endemic to Asia, were first reported in the San Francisco Bay in 1992. They are now established in nearly all San Francisco Bay tributaries. These crabs accumulate more metals, such as mercury, than crustaceans living in the water column. Because their predators include fish, birds, mammals and humans, their mercury burdens have an exceptional potential to impact the ecosystem and public health. We sought to elucidate the potential threat of mitten crab mercury burdens in three adjacent streams in southern San Francisco Bay, one of which is known to be contaminated with mercury. Mitten crabs had hepatopancreas concentrations of total mercury and methylmercury that did not differ among streams. The maximum burden we measured was below the action level of 1 ppm recommended by the USEPA. Hepatopancreas concentrations of methylmercury declined with increasing crab size, suggesting a mechanism for mercury excretion and that predators might reduce mercury exposure if they select larger crabs. Because mercury may be heterogeneously distributed among tissues, estimation of the impacts of crab mercury burdens on the environment requires more data on the feeding preferences of predators. Hepatopancreas concentrations of mercury decline with crab size, which may have important consequences for bio-magnification in food webs. ?? 2004 Elsevier Ltd. All rights reserved.

Environmental Pollution↗

Re-prioritization of the U.S. Geological Survey Federal Priority Streamgage Network, 2022

The Federal Priority Streamgage (FPS) network of the U.S. Geological Survey (USGS), created in 1999 as the National Streamflow Information Program, receives Congressional appropriations to support the operation of a federally-funded “backbone” network of streamflow gages across the United States that are designated to meet the “Federal needs” or priorities of the country. Anticipating the evolution of Federal stakeholder water-data needs, the USGS launched a re-evaluation of the fundamental priorities for the FPS network in October 2020. In March 2022, the FPS Re-Prioritization Project used an online survey to solicit feedback from 767 stakeholders representing 22 Federal agencies who benefit from the FPS network. Additional feedback from survey respondents was obtained during online listening sessions to validate the USGS’s understanding of current Federal water-data needs. Results of the feedback show that the original five network priorities identified by the U.S. Geological Survey in 1999 are still valid but require modification to better incorporate additional needs, including Federal water operations, streamflow trends and extremes, water rights involving Federal lands, and streamflow data supporting ecosystem health. Federal stakeholder feedback also indicated that the inclusion of precipitation and water-temperature data collection, along with stream imagery, would enhance the value of the FPS network. Results of the FPS Re-Prioritization Project and Open Season that ended in May 2024 revealed that the number of FPS locations meeting the updated eligibility criteria nearly tripled, which illustrates the value of the information provided by the FPS network. The Water Forecasting & Operations and the Water Quality network priorities contributed to the largest number of new eligible FPS sites, demonstrating the importance of the FPS network in supporting informed decisions related to the protection of life, property, the environment, and the economy of the United States.

Open-File Report↗

Monitoring forest changes in the southwestern United States using multitemporal Landsat data

Landsat time series data sets were acquired for the Santa Fe National Forest in New Mexico. This area includes the San Pedro Parks Wilderness area, which was designated as an official wilderness in 1964. Eight autumnal Landsat Thematic Mapper (TM) scenes acquired from 1988 to 2006 were analyzed to determine whether significant changes have occurred throughout the region during the past 18 years and, if so, to assess whether the changes are long-term and gradual or short-term and abrupt. It was found that, starting in about 1995, many of the conifer stands within the Wilderness area showed consistently gradual and marked increases in the Shortwave Infrared/Near Infrared Index. These trends generally imply decreases in canopy greenness or increases in mortality. Other high-elevation conifer forests located outside of the Wilderness area showed similar spectral trends, indicating that changes are potentially widespread. The spatial patterns of forest damage as inferred from the image analyses were very similar to the general patterns of insect defoliation damage mapped via aerial sketch mapping by the United States Department of Agriculture Forest Service Forest Health Monitoring Program. A field visit indicated that zones of spectral change are associated with high levels of forest damage and mortality, likely caused by a combination of insects and drought. The study demonstrates the effectiveness of using historical Landsat data for providing objective and consistent long-term assessments of the gradual ecosystem changes that are occurring within the western United States.

Remote Sensing of Environment↗

Divergent gene expression profiles in Alaskan sea otters: An indicator of chronic domoic acid exposure?

An opportunistic investigation into ecosystem instability in Kachemak Bay (KBay), Alaska, has led us to investigate exposure to toxic algae in sea otters. We used gene expression to explore the physiological health of sea otters sampled in KBay in May 2019. We found altered levels of gene transcripts in comparison with reference sea otters from clinically normal, oil-exposed, and nutritionally challenged populations sampled over the past decade. KBay sea otters were markedly divergent from the other groups for five genes, which indicated the involvement of neurological, cardiac, immune, and detoxification systems. Further, analyses of urine and fecal samples detected domoic acid in the KBay sea otters. In combination, these results may point to chronic, low-level exposure to an algal toxin, such as domoic acid. With a warming climate, the frequency and severity of harmful algal blooms in marine environments is anticipated to increase, and novel molecular technologies to detect sublethal or chronic exposure to algal toxins will help provide an early warning of threats to the stability of populations and ecosystems.

Alaska↗

Polar organic chemical integrative sampling and liquid chromatography- electrospray/ion-trap mass spectrometry for assessing selected prescription and illicit drugs in treated sewage effluents

The purpose of the research presented in this paper was twofold: (1) to demonstrate the coupling of two state-of-the-art techniques: a time-weighted polar organic chemical integrative sampler (POCIS) and microliquid chromatography–electrospray/ion-trap mass spectrometry and (2) to assess the ability of these methodologies to detect six drugs (azithromycin, fluoxetine, omeprazole, levothyroxine, methamphetamine, methylenedioxymethamphetamine [MDMA]) in a real-world environment, e.g., waste water effluent. In the effluent from three wastewater treatment plants (WWTPs), azithromycin was detected at concentrations ranging from 15 to 66 ng/L, which is equivalent to a total annual release of 1 to 4 kg into receiving waters. Detected and confirmed in the effluent from two WWTPs were two illicit drugs, methamphetamine and MDMA, at 2 and 0.5 ng/L, respectively. Although the ecotoxicologic significance of drugs in environmental matrices, particularly water, has not been closely examined, it can only be surmised that these substances have the potential to adversely affect biota that are continuously exposed to them even at very low levels. The potential for chronic effects on human health is also unknown but of increasing concern because of the multiuse character of water, particularly in densely populated, arid areas.

Archives of Environmental Contamination and Toxico↗

Landowners' cognitions and motivations coupled with practice durability influence persistence in grazing agricultural conservation practices in southwest Virginia

Agricultural conservation practices are often used to protect stream health while continuing food production. However, recovery of stream health is often not as rapid or extensive as planned. The efficacy of practices may be improved by promoting their continued use by landowners (i.e., persistence) after cost-share contracts with government agencies end. Persistence rates and their drivers are typically unknown. Therefore, we mail-surveyed 889 landowners to understand persistence in grazing practices in southwest Virginia. Survey responses were analyzed quantitatively using logistic regression and qualitatively via coding. We found that persistence rates for vegetative practices, cattle-exclusion fencing, off-stream watering structures, and pasture management were 74%, 84%, 94%, and 94%, respectively. Both the quantitative and qualitative analyses indicated that landowner cognitions (i.e., attitudes toward practices, environment, and agencies), environmental motivations, and practice durability influence persistence. Our results highlight that persistence could be encouraged by: (1) providing targeted messaging that demonstrates persistence benefits and aligns with landowners' motivations, (2) ensuring that responsibility to maintain practices is transferred during land-tenure changes, and (3) allocating more agency funding to practice maintenance.

Virginia↗

Coral calcification in a changing ocean

Animals and plants that live in the ocean form skeletons and other hard parts by combining calcium ions and carbonate ions to create calcium carbonate. This process is called calcification. In tropical and subtropical oceans, the calcification of corals and other organisms creates reefs that protect islands, produce beautiful white-sand beaches, and create habitat for thousands of species that live on coral reefs. Many reefs around the globe are declining in health. Live, reef-building corals are becoming scarcer, often being replaced by fleshy algae (seaweeds) that do not build reefs. Many factors contribute to reef decline, but scientific consensus is that coral bleaching, coral disease, overfishing, and coastal development are largely to blame. It is the role of the U.S. Geological Survey (USGS) to provide science that informs the decision-making process of those who are tasked with managing reef resources under U.S. jurisdiction. One of the goals of the Coral Reef Ecosystem Studies (CREST) project is to examine how calcification rates in reef-building corals and encrusting coralline algae are changing in response to changes in the ocean environment.

Fact Sheet↗

Routine screening of harmful microorganisms in beach sands: implications to public health

Beaches worldwide provide recreational opportunities to hundreds of millions of people and serve as important components of coastal economies. Beach water is often monitored for microbiological quality to detect the presence of indicators of human sewage contamination so as to prevent public health outbreaks associated with water contact. However, growing evidence suggests that beach sand can harbor microbes harmful to human health, often in concentrations greater than the beach water. Currently, there are no standards for monitoring, sampling, analyzing, or managing beach sand quality. In addition to indicator microbes, growing evidence has identified pathogenic bacteria, viruses, and fungi in a variety of beach sands worldwide. The public health threat associated with these populations through direct and indirect contact is unknown because so little research has been conducted relating to health outcomes associated with sand quality. In this manuscript, we present the consensus findings of a workshop of experts convened in Lisbon, Portugal to discuss the current state of knowledge on beach sand microbiological quality and to develop suggestions for standardizing the evaluation of sand at coastal beaches. The expert group at the “Microareias 2012” workshop recommends that 1) beach sand should be screened for a variety of pathogens harmful to human health, and sand monitoring should then be initiated alongside regular water monitoring; 2) sampling and analysis protocols should be standardized to allow proper comparisons among beach locations; and 3) further studies are needed to estimate human health risk with exposure to contaminated beach sand. Much of the manuscript is focused on research specific to Portugal, but similar results have been found elsewhere, and the findings have worldwide implications.

Science of the Total Environment↗

Alternative tissue analysis method developed for organochlorine contaminants in aquatic organisms

The exposure of aquatic life to organochlorine contaminants has been investigated during the past two decades because of human and ecosystem health concerns related to the bioaccumulation of hazardous, lipophilic substances. The toxic effects of polychlorodibenzo-12-dioxins and polychlorinated biphenyls (PCBs) are well known, and recent evidence also suggests that low level exposure to lipophilic organochlorines may interfere with normal development during sensitive early life history stages (Hileman 1993). As the use of lipophilic organochlorines, such as DDT, in third world countries continues and with the purported global cycling (Bidleman and Olney 1974; Tanabe et al. 1983) and food chain accumulation (Thomann and Connoly 1984, Thomann 1989) of persistent organochlodnes, the occurrence of these compounds in aquatic organisms is a critical global environmental issue. An understanding of the fate of organochlorines in the environment clearly remains an extremely important subject related to water quality. The U.S. Geological Survey (USGS) has recently gained congressional approval in the United States to track nation wide trends in water quality through the establishment of the National Water Quality Assessment Program (NAWQA). Among the goals defined by NAWQA, aquatic organisms, including fish, shellfish, and plants, collected from major drainage basins will be analyzed for, along with other contaminants, polychlorinated biphenyls (PCBs), organochlorine insecticides, and chlorobenzenes (Crawford and Luoma 1992). The purpose of this report is to present quality assurance data obtained from the development of a PCB, chlorobenzene, and organochlorine insecticide tissue analysis method in support of NAWQA and other large-scale water quality programs conducted through our laboratory.

Bulletin of Environmental Contamination and Toxico↗

The Nitrogen Footprint Tool network: A multi-institution program to reduce nitrogen pollution

Anthropogenic sources of reactive nitrogen have local and global impacts on air and water quality and detrimental effects on human and ecosystem health. This paper uses the nitrogen footprint tool (NFT) to determine the amount of nitrogen (N) released as a result of institutional consumption. The sectors accounted for include food (consumption and upstream production), energy, transportation, fertilizer, research animals, and agricultural research. The NFT is then used for scenario analysis to manage and track reductions, which are driven by the consumption behaviors of both the institution itself and its constituent individuals. In this paper, the first seven completed institution nitrogen footprint results are presented. The institution NFT network aims to develop footprints for many institutions to encourage widespread upper-level management strategies that will create significant reductions in reactive nitrogen released to the environment. Energy use and food purchases are the two largest sectors contributing to institution nitrogen footprints. Ongoing efforts by institutions to reduce greenhouse gas emissions also help to reduce the nitrogen footprint, but the impact of food production on nitrogen pollution has not been directly addressed by the higher-ed sustainability community. The NFT Network found that institutions could reduce their nitrogen footprints by optimizing food purchasing to reduce consumption of animal products and minimize food waste, as well as reducing dependence on fossil fuels for energy.

Sustainability↗