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Temporal segregation in spawning between native Yellowstone cutthroat trout and introduced rainbow trout

Hybridisation with introduced taxa poses a threat to native fish populations. Mechanisms of reproductive isolation can limit or prevent hybridisation between closely related species. Understanding how these mechanisms interact between the same species across geographically distinct occurrences of secondary contact, and how regional factors influence them, can inform our understanding of hybridisation as a threat and management actions to mitigate this threat. We used data collected on adult fish migration timing and approximate emergence timing of subsequent juvenile fish paired with genomic data to assess whether temporal isolation in the timing of spawning exists between Yellowstone cutthroat trout, rainbow trout and hybrids in the North Fork Shoshone River drainage in northwest Wyoming. We found evidence that Yellowstone cutthroat trout spawn, on average, two to four weeks later than rainbow trout and hybrids and two environmental covariates related to water temperature and discharge were associated with differences in spawning migration timing. Despite statistical support for Yellowstone cutthroat trout spawning later, disproportionately high numbers of rainbow trout and hybrids, paired with extended spawning seasons, lead to substantial overlap between all genotypes. Our results provide further evidence of temporal segregation in the timing of spawning as a mechanism of reproductive isolation between closely related species, but substantial spawning overlap suggests temporal segregation alone will not be enough to curtail hybridisation in conservation populations.

Wyoming↗

Growth responses of subalpine fir to climatic variability in the Pacific Northwest

We studied regional variation in growth-limiting factors and responses to climatic variability in subalpine forests by analyzing growth patterns for 28 tree-ring growth chronologies from subalpine fir ( Abies lasiocarpa (Hook.) Nutt.) stands in the Cascade and Olympic Mountains (Washington and Oregon, U.S.A.). Factor analysis identified four distinct time series of common growth patterns; the dominant growth pattern at any site varied with annual precipitation and temperature (elevation). Throughout much of the region, growth is negatively correlated with winter precipitation and spring snowpack depth, indicating that growth is limited primarily by short growing seasons. On the driest and warmest sites, growth is negatively correlated with previous summer temperature, suggesting that low summer soil moisture limits growth. Growth patterns in two regions were sensitive to climatic variability associated with the Pacific Decadal Oscillation, apparently responding to low-frequency variation in spring snowpack and summer soil moisture (one negatively, one positively). This regional-scale analysis shows that subalpine fir growth in the Cascades and Olympics is limited by different climatic factors in different subregional climates. Climate–growth relationships are similar to those for a co-occurring species, mountain hemlock ( Tsuga mertensiana (Bong.) Carrière), suggesting broad biogeographic patterns of response to climatic variability and change by subalpine forest ecosystems in the Pacific Northwest.

Oregon, Washington↗

Western water and climate change

The western United States is a region long defined by water challenges. Climate change adds to those historical challenges, but does not, for the most part, introduce entirely new challenges; rather climate change is likely to stress water supplies and resources already in many cases stretched to, or beyond, natural limits. Projections are for continued and, likely, increased warming trends across the region, with a near certainty of continuing changes in seasonality of snowmelt and streamflows, and a strong potential for attendant increases in evaporative demands. Projections of future precipitation are less conclusive, although likely the northernmost West will see precipitation increases while the southernmost West sees declines. However, most of the region lies in a broad area where some climate models project precipitation increases while others project declines, so that only increases in precipitation uncertainties can be projected with any confidence. Changes in annual and seasonal hydrographs are likely to challenge water managers, users, and attempts to protect or restore environmental flows, even where annual volumes change little. Other impacts from climate change (e.g., floods and water-quality changes) are poorly understood and will likely be location dependent. In this context, four iconic river basins offer glimpses into specific challenges that climate change may bring to the West. The Colorado River is a system in which overuse and growing demands are projected to be even more challenging than climate-change-induced flow reductions. The Rio Grande offers the best example of how climate-change-induced flow declines might sink a major system into permanent drought. The Klamath is currently projected to face the more benign precipitation future, but fisheries and irrigation management may face dire straits due to warming air temperatures, rising irrigation demands, and warming waters in a basin already hobbled by tensions between endangered fisheries and agricultural demands. Finally, California's Bay-Delta system is a remarkably localized and severe weakness at the heart of the region's trillion-dollar economy. It is threatened by the full range of potential climate-change impacts expected across the West, along with major vulnerabilities to increased flooding and rising sea levels.

Ecological Applications↗

Analysis of ambient conditions and simulation of hydrodynamics and water-quality characteristics in Beaver Lake, Arkansas, 2001 through 2003

Beaver Lake is a large, deep-storage reservoir located in the upper White River Basin in northwestern Arkansas. The purpose of this report is to describe the ambient hydrologic and water-quality conditions in Beaver Lake and its inflows and describe a two-dimensional model developed to simulate the hydrodynamics and water quality of Beaver Lake from 2001 through 2003. Water-quality samples were collected at the three main inflows to Beaver Lake; the White River near Fayetteville, Richland Creek at Goshen, and War Eagle Creek near Hindsville. Nutrient concentrations varied among the tributaries because of land use and contributions of nutrients from point sources. The median concentrations of total ammonia plus organic nitrogen were greater for the White River than Richland and War Eagle Creeks. The greatest concentrations of nitrite plus nitrate and total nitrogen, however, were observed at War Eagle Creek. Phosphorus concentrations were relatively low, with orthophosphorus and dissolved phosphorus concentrations mostly below the laboratory reporting limit at the three sites. War Eagle Creek had significantly greater median orthophosphorus and total phosphorus concentrations than the White River and Richland Creek. Dissolved organic-carbon concentrations were significantly greater at the White River than at War Eagle and Richland Creeks. The White River also had significantly greater turbidity than War Eagle Creek and Richland Creek. The temperature distribution in Beaver Lake exhibits the typical seasonal cycle of lakes and reservoirs located within similar latitudes. Beaver Lake is a monomictic system, in which thermal stratification occurs annually during the summer and fall and complete mixing occurs in the winter. Isothermal conditions exist throughout the winter and early spring. Nitrogen concentrations varied temporally, longitudinally, and vertically in Beaver Lake for 2001 through 2003. Nitrite plus nitrate concentrations generally decreased from the upstream portion of Beaver Lake to the downstream portion and generally were greater in the hypolimnion. Total ammonia plus organic nitrogen concentrations also decreased from the upstream end of Beaver Lake to the downstream end and were substantially greater in the hypolimnion of Beaver Lake. Phosphorus concentrations mostly were near or below laboratory detection limits in the epilimnion and metalimnion in Beaver Lake and were substantially greater in the hypolimnion in the upstream and middle parts of the reservoir. Measured total and dissolved organic carbon in Beaver Lake was relatively uniform spatially, longitudinally, and vertically in the reservoir from January 2001 through December 2003. Chlorophyll a concentrations measured at sites in the upstream portion of the lake were significantly greater than at the other sites in the downstream portion of Beaver Lake. During the study period, water clarity in Beaver Lake was significantly greater at the downstream end of the reservoir than at the upstream end. The greatest Secchi depths at the downstream end of the reservoir generally were observed in 2001 compared to 2002 and 2003, but did not have a seasonal pattern as observed at sites in the middle and upstream portion of the reservoir. Similar to Secchi depth results, turbidity results indicated greater water clarity in the downstream portion of Beaver Lake compared to the upstream portion. Turbidity also was greater in the hypolimnion than in the epilimnion in the reservoir during the stratification season. A two-dimensional, laterally averaged, hydrodynamic, and water-quality model using CE-QUAL-W2 Version 3.1 was developed for Beaver Lake and calibrated based on vertical profiles of temperature and dissolved oxygen, and water-quality constituent concentrations collected at various depths at four sites in the reservoir from April 2001 to April 2003. Simulated temperatures and dissolved-oxygen concentrations compared reasonably well with measured t

Scientific Investigations Report↗

Nutrient loading and selected water-quality and biological characteristics of Dickinson Bayou near Houston, Texas, 1995-97

Data were collected at 10 stations in the Dickinson Bayou watershed near Houston, Texas, from March 1995 through February 1997 to estimate the concentrations, loads, and yields of selected nutrients that enter the bayou; to characterize the effects on nutrient concentrations of flow conditions, seasonality, and land use; and to identify nutrient sources (point or nonpoint) inferred from the occurrence and abundance of algal species in the benthic algal community. These data included rainfall samples, streamflow measurements, stream-water-quality samples, and biological samples, in addition to quality-assurance/quality-control samples. Estimates of loads of selected nutrients for the 106-square-mile watershed during the study were made for point sources and nonpoint sources. Point-source loading data are available only for ammonia nitrogen. Approximately 21.3 pounds per day of ammonia nitrogen is estimated from point sources during the study period. Nonpoint-source loads are estimated for eight nutrient forms: 7.84 pounds per day of dissolved ammonia nitrogen, 5.79 pounds per day of dissolved nitrite nitrogen, 215 pounds per day of dissolved Kjeldahl nitrogen, 350 pounds per day of total Kjeldahl nitrogen, 40.1 pounds per day of dissolved nitrite plus nitrate nitrogen, 67.6 pounds per day of total phosphorus, 46.6 pounds per day of dissolved phosphorus, and 42.8 pounds per day of dissolved orthophosphate. Rainfall-deposition rates also are estimated for comparison with point- and nonpoint-source loads. Deposition rates are 110 pounds per day of dissolved ammonia nitrogen, 120 pounds per day of dissolved nitrate nitrogen, and 15.8 pounds per day of dissolved phosphorus. Statistical tests were used to determine whether there are significant differences between nutrient concentrations during low-flow and during high-flow conditions. For basins with rural/mixed and urban land uses, nutrient concentrations generally are significantly different (greater) during storm events than during low flow, indicating accumulation in the watershed and subsequent washoff of nutrients. However, nutrient concentrations in storm-event samples consisting predominantly of runoff from a pasture are not significantly greater than those in low-flow samples. Statistical tests for seasonality indicate that dissolved ammonia nitrogen is significantly different in at least one season for all land uses (rural/residential, rural/mixed, and pasture) except urban. Concentrations tend to increase in the spring and early summer months, possibly from fertilizer application and subsequent washoff. Constituent-yield data were used to make direct comparisons of the nonpoint-source load contributions from four stations with watersheds of different land use. These comparisons lead to three conclusions: (1) For all nutrient species except orthophosphate, urban land use is the largest nonpoint-source contributor, (2) Kjeldahl nitrogen is the most abundant nutrient species, and (3) organic nitrogen accounts for the major part of the Kjeldahl nitrogen. Algal samples were collected at seven stations and were analyzed for periphyton identification and enumeration, and chlorophyll a and chlorophyll b concentrations. The large relative abundance of soil algae at stations in the middle of the watershed likely indicates the cumulative effects on water quality of agricultural nonpoint sources. Farther downstream near the State Highway 3 bridge, and downstream of three major tributary inflows, the increase in abundance of soil algae to a larger-than-expected level might reflect water-quality influences from predominantly urban nonpoint sources in the drainage basins of the three major tributary inflows. Nutrient concentrations do not appear to limit algal production in the upper (non-tidal) reach of Dickinson Bayou; but nutrient concentrations could have been limiting benthicalgal production in the lower (tidal) reach of the bayou during the time of the synoptic survey. If nitrogen is the limiting resource for algal productivity in the tidal reach of Dickinson Bayou, eutrophication of the system could be (at least partially) mitigated if nonpoint-source nutrient loads into the Bayou were reduced.

Texas↗

Infectivity of wild-bird origin Influenza A viruses in Minnesota wetlands across seasons

The environmental tenacity of influenza A viruses (IAVs) in the environment likely plays a role in their transmission; IAVs are able to remain infectious in aquatic habitats and may have the capacity to seed outbreaks when susceptible wild bird hosts utilize these same environments months or even seasons later. Here, we aimed to assess the persistence of low-pathogenicity IAVs from naturally infected ducks in Northwestern Minnesota through a field experiment. Viral infectivity was measured using replicate samples maintained in distilled water in a laboratory setting as well as in filtered water from four natural water bodies maintained in steel perforated drums (hereafter, mesocosms) within the field from autumn 2020 to spring 2021. There was limited evidence for the extended persistence of IAVs held in mesocosms; from 65 initial IAV-positive samples, only six IAVs persisted to at least 202 days in the mesocosms compared to 17 viruses persisting at least this long when held under temperature-controlled laboratory settings in distilled water. When accounting for the initial titer of samples, viruses detected at a higher concentration at the initiation of the experiment persisted longer than those with a lower starting titer. A parallel experimental laboratory model was used to further explore the effects of water type on viral persistence, and the results supported the finding of reduced tenacity of IAVs held in mesocosms compared to distilled water. The results of this investigation provide evidence that many factors, including temperature and physicochemical properties, impact the duration of viral infectivity in natural settings, further extending our understanding of the potential and limitations of environmental-based methodologies to recover infectious IAVs.

Minnesota↗

Hydrogeologic Framework, Groundwater Movement, and Water Budget in Tributary Subbasins and Vicinity, Lower Skagit River Basin, Skagit and Snohomish Counties, Washington

A study to characterize the groundwater-flow system in four tributary subbasins and vicinity of the lower Skagit River basin was conducted by the U.S. Geological Survey to assist Skagit County and the Washington State Department of Ecology in evaluating the effects of potential groundwater withdrawals and consumptive use on tributary streamflows. This report presents information used to characterize the groundwater and surface-water flow system in the subbasins, and includes descriptions of the geology and hydrogeologic framework of the subbasins; groundwater recharge and discharge; groundwater levels and flow directions; seasonal groundwater-level fluctuations; interactions between aquifers and the surface-water system; and a water budget for the subbasins. The study area covers about 247 mi2 along the Skagit River and its tributary subbasins (East Fork Nookachamps Creek, Nookachamps Creek, Carpenter Creek, and Fisher Creek) in southwestern Skagit County and northwestern Snohomish County, Washington. The geology of the area records a complex history of accretion along the continental margin, mountain building, deposition of terrestrial and marine sediments, igneous intrusion, and the repeated advance and retreat of continental glaciers. A simplified surficial geologic map was developed from previous mapping in the area, and geologic units were grouped into nine hydrogeologic units consisting of aquifers and confining units. A surficial hydrogeologic unit map was constructed and, with lithologic information from 296 drillers'logs, was used to produce unit extent and thickness maps and four hydrogeologic sections. Groundwater in unconsolidated aquifers generally flows towards the northwest and west in the direction of the Skagit River and Puget Sound. This generalized flow pattern is likely complicated by the presence of low-permeability confining units that separate discontinuous bodies of aquifer material and act as local groundwater-flow barriers. Groundwater-flow directions in the sedimentary aquifer likely reflect local topographic relief (radial flow from bedrock highs) and more regional westward flow from the mountains to the Puget Sound. The largest groundwater-level fluctuations observed during the monitoring period (October 2006 through September 2008) occurred in wells completed in the sedimentary aquifer, and ranged from about 3 to 27 feet. Water levels in wells completed in unconsolidated hydrogeologic units exhibited seasonal variations ranging from less than 1 to about 10 feet. Synoptic streamflow measurements made in August 2007 and June 2008 indicate a total groundwater discharge to creeks in the tributary subbasin area of about 13.15 and 129.6 cubic feet per second (9,520 and 93,830 acre-feet per year), respectively. Streamflow measurements illustrate a general pattern in which the upper reaches of creeks in the study area tended to gain flow from the groundwater system, and lower creek reaches tended to lose water. Large inflows from tributaries to major creeks in the study area suggest the presence of groundwater discharge from upland areas underlain by bedrock. The groundwater system within the subbasins received an average (September 1, 2006 to August 31, 2008) of about 92,400 acre-feet or about 18 inches of recharge from precipitation a year. Most of this recharge (65 percent) discharges to creeks, and only about 3 percent is withdrawn from wells. The remaining groundwater recharge (32 percent) leaves the subbasin groundwater system as discharge to the Skagit River and Puget Sound.

Scientific Investigations Report↗

Seasonal distribution of Gulf of Mexico sturgeon in the pensacola bay system, Florida

Temporal and spatial distributions of Gulf of Mexico (Gulf) sturgeon Acipenser oxyrinchus desotoi were assessed in the Pensacola bay system, Florida, using stationary ultrasonic telemetry. Fifty‐eight Gulf sturgeon were tagged within the Escambia (n = 26), Yellow (n = 8), Blackwater (n = 12) and Choctawhatchee Rivers (n = 12) in June, July, September and October, 2005. Fifty‐four Gulf sturgeon were detected at least once during the study. Migration of sturgeon occurred throughout the bay system in fall, to various winter habitats in the Gulf of Mexico and Santa Rosa Sound. In spring, tagged sturgeon migrated back through the bay system to summer habitats in rivers. Based on the duration and number of detections, Gulf sturgeon primarily used the upper portions of East and Escambia Bays as migration routes in and out of all rivers during spring and summer and inhabited the lower portion of Pensacola Bay for longer durations in fall and winter. Specific areas within the Pensacola bay system were used in summer and winter that were not previously documented as essential sturgeon habitat. Areas in southeastern Pensacola Bay were heavily used during winter by a large portion of the population. Gulf sturgeon also exhibited long‐term winter residency in Santa Rosa Sound for two consecutive years. An area in northeastern Escambia Bay supported Gulf sturgeon during the summer, which was unexpected and can not be explained by the data from this study. However, the discovery that Gulf sturgeon remain in the bay during the summer has important ecological and management implications that need further investigation.

Florida↗

Assessing historical and projected carbon balance of Alaska: A synthesis of results and policy/management implications

We summarize the results of a recent interagency assessment of land carbon dynamics in Alaska, in which carbon dynamics were estimated for all major terrestrial and aquatic ecosystems for the historical period (1950–2009) and a projection period (2010–2099). Between 1950 and 2009, upland and wetland (i.e., terrestrial) ecosystems of the state gained 0.4 Tg C/yr (0.1% of net primary production, NPP), resulting in a cumulative greenhouse gas radiative forcing of 1.68 × 10 −3 W/m 2 . The change in carbon storage is spatially variable with the region of the Northwest Boreal Landscape Conservation Cooperative (LCC) losing carbon because of fire disturbance. The combined carbon transport via various pathways through inland aquatic ecosystems of Alaska was estimated to be 41.3 Tg C/yr (17% of terrestrial NPP). During the projection period (2010–2099), carbon storage of terrestrial ecosystems of Alaska was projected to increase (22.5–70.0 Tg C/yr), primarily because of NPP increases of 10–30% associated with responses to rising atmospheric CO 2 , increased nitrogen cycling, and longer growing seasons. Although carbon emissions to the atmosphere from wildfire and wetland CH 4 were projected to increase for all of the climate projections, the increases in NPP more than compensated for those losses at the statewide level. Carbon dynamics of terrestrial ecosystems continue to warm the climate for four of the six future projections and cool the climate for only one of the projections. The attribution analyses we conducted indicated that the response of NPP in terrestrial ecosystems to rising atmospheric CO 2 (~5% per 100 ppmv CO 2 ) saturates as CO 2 increases (between approximately +150 and +450 ppmv among projections). This response, along with the expectation that permafrost thaw would be much greater and release large quantities of permafrost carbon after 2100, suggests that projected carbon gains in terrestrial ecosystems of Alaska may not be sustained. From a national perspective, inclusion of all of Alaska in greenhouse gas inventory reports would ensure better accounting of the overall greenhouse gas balance of the nation and provide a foundation for considering mitigation activities in areas that are accessible enough to support substantive deployment.

Alaska↗

Using diatom assemblages to assess urban stream conditions

We characterized changes in diatom assemblages along an urban-to-rural gradient to assess impacts of urbanization on stream conditions. Diatoms, water chemistry, and physical variables of riffles at 19 urban and 28 rural stream sites were sampled and assessed during the summer base flow period. Near stream land use was characterized using GIS. In addition, one urban and one rural site were sampled monthly throughout a year to assess temporal variation of diatom assemblages between the urban and rural stream sites. Canonical correspondence analysis (CCA) showed that the 1st ordination axis distinctly separated rural and urban sites. This axis was correlated with conductivity (r = 0.75) and % near-stream commercial/industrial land use (r = 0.55). TWINSPAN classified all sites into four groups based on diatom assemblages. These diatom-based site groups were significantly different in water chemistry (e.g., conductivity, dissolved nutrients), physical habitat (e.g., % stream substrate as fines), and near-stream land use. CCA on the temporal diatom data set showed that diatom assemblages had high seasonal variation along the 2nd axis in both urban and rural sites, however, rural and urban sites were well separated along the 1st ordination axis. Our results suggest that changes in diatom assemblages respond to urban impacts on stream conditions. ?? Springer 2006.

Hydrobiologia↗

On the relative roles of hydrology, salinity, temperature, and root productivity in controlling soil respiration from coastal swamps (freshwater)

Background and aims Soil CO 2 emissions can dominate gaseous carbon losses from forested wetlands (swamps), especially those positioned in coastal environments. Understanding the varied roles of hydroperiod, salinity, temperature, and root productivity on soil respiration is important in discerning how carbon balances may shift as freshwater swamps retreat inland with sea-level rise and salinity incursion, and convert to mixed communities with marsh plants. Methods We exposed soil mesocosms to combinations of permanent flooding, tide, and salinity, and tracked soil respiration over 2 1/2 growing seasons. We also related these measurements to rates from field sites along the lower Savannah River, Georgia, USA. Soil temperature and root productivity were assessed simultaneously for both experiments. Results Soil respiration from mesocosms (22.7-1678.2 mg CO 2 m -2 h -1 ) differed significantly among treatments during four of the seven sampling intervals, where permanently flooded treatments contributed to low rates of soil respiration and tidally flooded treatments sometimes contributed to higher rates. Permanent flooding reduced the overall capacity for soil respiration as soils warmed. Salinity did reduce soil respiration at times in tidal treatments, indicating that salinity may affect the amount of CO 2 respired with tide more strongly than under permanent flooding. However, soil respiration related greatest to root biomass (mesocosm) and standing root length (field); any stress reducing root productivity (incl. salinity and permanent flooding) therefore reduces soil respiration. Conclusions Overall, we hypothesized a stronger, direct role for salinity on soil respiration, and found that salinity effects were being masked by varied capacities for increases in respiration with soil warming as dictated by hydrology, and the indirect influence that salinity can have on plant productivity.

Georgia↗

Spatiotemporal variation in occurrence and co-occurrence of pesticides, hormones, and other organic contaminants in rivers in the Chesapeake Bay Watershed, United States

Investigating the spatiotemporal dynamics of contaminants in surface water is crucial to better understand how introduced chemicals are interacting with and potentially influencing aquatic organisms and environments. Within the Chesapeake Bay Watershed, USA, there are concerns about the potential role of contaminant exposure on fish health. Evidence suggests that exposure to contaminants in surface water is causing immunosuppression and intersex in freshwater fish species. Despite these concerns, there is a paucity of information regarding the complex dynamics of contaminant occurrence and co-occurrence in surface water across both space and time. To address these concerns, we applied a Bayesian hierarchical joint-contaminant model to describe the occurrence and co-occurrence patterns of 28 contaminants and total estrogenicity across six river sites and over three years. We found that seasonal occurrence patterns varied by contaminant, with the highest occurrence probabilities during the spring and summer months. Additionally, we found that the proportion of agricultural landcover in the immediate catchment, as well as stream discharge, did not have a significant effect on the occurrence probabilities of most compounds. Four pesticides (atrazine, metolachlor, fipronil and simazine) co-occurred across sites after accounting for environmental covariates. These results provide baseline information on the contaminant occurrence patterns of several classes of compounds within the Chesapeake Bay Watershed. Understanding the spatiotemporal dynamics of contaminants in surface water is the first step in investigating the effects of contaminant exposure on fisheries and aquatic environments.

Delaware, Maryland, New York, Pennsylvania, Virgin↗

Effects of permafrost melting on CO2 and CH4 exchange of a poorly drained black spruce lowland

[1] Permafrost melting is occurring in areas of the boreal forest region where large amounts of carbon (C) are stored in organic soils. We measured soil respiration, net CO 2 flux, and net CH 4 flux during May–September 2003 and March 2004 in a black spruce lowland in interior Alaska to better understand how permafrost thaw in poorly drained landscapes affects land‐atmosphere CO 2 and CH 4 exchange. Sites included peat soils underlain by permafrost at ∼0.4 m depth (permafrost plateau, PP), four thermokarst wetlands (TW) having no permafrost in the upper 2.2 m, and peat soils bordering the thermokarst wetlands having permafrost at ∼0.5 m depth (thermokarst edges, TE). Soil respiration rates were not significantly different among the sites, and 5‐cm soil temperature explained 50–91% of the seasonal variability in soil respiration within the sites. Groundcover vegetation photosynthesis (calculated as net CO 2 minus soil respiration) was significantly different among the sites (TW > TE > PP), which can be partly attributed to the difference in photosynthetically active radiation reaching the ground at each site type. Methane emission rates were 15 to 28 times greater from TW than from TE and PP. We modeled annual soil respiration and groundcover vegetation photosynthesis using soil temperature and radiation data, and CH 4 flux by linear interpolation. We estimated all sites as net C gas sources to the atmosphere (not including tree CO 2 uptake at PP and TE), although the ranges in estimates when accounting for errors were large enough that TE and TW may have been net C sinks.

Journal of Geophysical Research G: Biogeosciences↗

Seasonal habitat use of brook trout and juvenile steelhead in a Lake Ontario tributary

Brook trout ( Salvelinus fontinalis ) are generally restricted to headwaters in New York tributaries of Lake Ontario. In only a few streams are brook trout abundant in lower stream reaches that are accessible to adult Pacific salmonids migrating from the lake. Consequently, because of the rarity of native brook trout populations in these lower stream reaches it is important to understand how they use stream habitat in sympatry with juvenile Pacific salmonids which are now naturalized in several Lake Ontario tributaries. In this study, we examined the seasonal (spring, summer, and fall) habitat use of brook trout and juvenile steelhead ( Oncorhynchus mykiss ) in Hart Brook, a tributary of eastern Lake Ontario. We found interspecific, intraspecific, and seasonal variation in habitat use. Subyearling steelhead were associated with faster water velocities than subyearling brook trout and, overall, had the least habitat similarity to the other salmonid groups examined. Overyearling brook trout and yearling steelhead exhibited the greatest degree of habitat selection and habitat selection by all four salmonid groups was greatest in summer. The availability of pool habitat for overyearling salmonids may pose the largest impediment to these species in Hart Brook.

Journal of Freshwater Ecology↗

Nesting ecology and reproductive rate of the red-winged blackbird in tidal marshes of the upper Chesapeake Bay region

The nesting ecology and reproductive rate of the polygynous red-winged blackbird, Agelaius phoeniceus, were studied in the tidal marshes of Maryland during the period of 1958 through 1961. Sixteen nesting colonies were located in six major marsh communities of the Eastern Shore and were visited approximately twice a week from late April to mid-August. The average clutch size for 537 clutches was 3.3 eggs, with a range of 2 to 5 eggs. The ratio of territorial males to nesting females was 1:1.9. There was direct evidence of double broods by four females. The average number of young produced was 4.2 per breeding female or 8.1 per breeding male. Nesting success for the total of 675 active nests was 57%, with a range from 38% to 69% in the colonies. Robust plants that held constant form throughout the nesting season supported 95% of the nests, and the success of these nests was 58%; in contrast, non-robust plants supported only 5% of the nests, and success of this group of nests was only 26%. Nesting success varied with height from ground: 45% for nests less than 2 feet from the ground, 55% for those 2 to 4 feet, and 62% for those more than 4 feet. Histories of 749 nests were summarized by stages: 749 newly built, 675 with eggs, 424 with nestlings, and 388 with fledging young.

Chesapeake Bay region↗

Sample size and allocation of effort in point count sampling of birds in bottomland hardwood forests

To examine sample size requirements and optimum allocation of effort in point count sampling of bottomland hardwood forests, we computed minimum sample sizes from variation recorded during 82 point counts (May 7-May 16, 1992) from three localities containing three habitat types across three regions of the Mississippi Alluvial Valley (MAV). Also, we estimated the effect of increasing the number of points or visits by comparing results of 150 four-minute point counts obtained from each of four stands on Delta Experimental Forest (DEF) during May 8-May 21, 1991 and May 30-June 12, 1992. For each stand, we obtained bootstrap estimates of mean cumulative number of species each year from all possible combinations of six points and six visits. ANOVA was used to model cumulative species as a function of number of points visited, number of visits to each point, and interaction of points and visits. There was significant variation in numbers of birds and species between regions and localities (nested within region); neither habitat, nor the interaction between region and habitat, was significant. For a = 0.05 and a = 0.10, minimum sample size estimates (per factor level) varied by orders of magnitude depending upon the observed or specified range of desired detectable difference. For observed regional variation, 20 and 40 point counts were required to accommodate variability in total individuals (MSE = 9.28) and species (MSE = 3.79), respectively, whereas ? 25 percent of the mean could be achieved with five counts per factor level. Sample size sufficient to detect actual differences of Wood Thrush (Hylocichla mustelina) was >200, whereas the Prothonotary Warbler (Protonotaria citrea) required <10 counts. Differences in mean cumulative species were detected among number of points visited and among number of visits to a point. In the lower MAV, mean cumulative species increased with each added point through five points and with each additional visit through four visits. Although no interaction was detected between number of points and number of visits, when paired reciprocals were compared, more points invariably yielded a significantly greater cumulative number of species than more visits to a point. Still, 36 point counts per stand during each of two breeding seasons detected only 52 percent of the known available species pool in DEF.

Book chapter↗

The reproductive success of lake herring in habitats near shipping channels and ice-breaking operations in the St. Marys River, Michigan, USA

A study of the reproductive success of lake herring ( Coregonus artedi ) in the St. Marys River was conducted in the winters and springs of 1994, 1995, and 1996. The St. Marys River connects Lake Superior to the lower Great Lakes making it an important route for ship traffic. Recent pressure by commercial carriers to extend the shipping season by breaking ice earlier in spring, has raised concerns over the possible adverse effects on lake herring reproduction in the river caused by increased turbidity associated with vessel passage. Lake herring spawn in fall and their eggs overwinter under ice cover on the bottom of the St. Marys River. Hatching occurs in the spring after ice-out when water temperatures rise. Specialized incubators were used to hold fertilized lake herring eggs at four experimental sites, chosen to represent the range of various bottom substrate types of the St. Marys River from boulder rock reefs to soft sediments. In winter, incubators were placed under the ice on the bottom of the river at three sites each year. After ice-out, sites were relocated, and the incubators were retrieved and opened to determine the number of live and dead lake herring eggs and larvae. Survival was consistent from year to year at each site with the lowest survival percentage found at the site with the softest sediments, directly adjacent to the St. Marys River channel and downstream of the mouth of the Charlotte River. River bottom type and geographic location were the most important factors in determining egg survival. Sampling for indigenous larval lake herring was done throughout the spring hatching season in the areas adjacent to the incubator sites using nets and a diver-operated suction sampler. Result indicate that a small population (3) of larval lake herring was present throughout the sampling areas during the springs of 1994, 1995, and 1996 in the St. Marys River.

St. Marys River↗

Longitudinal and seasonal changes of organic matter sources through a semi-arid river-reservoir system

The quality and quantity of organic matter (OM) in a river system directly affects ecosystem health; thus, managers benefit from an in-depth understanding of the drivers and sources of OM. In the Snake River, a highly altered river-reservoir system in the semi-arid western United States, OM production and loading are key drivers of reservoir anoxia, which leads to several deleterious processes such as mercury methylation. However, sources and quantities of OM to the Snake River, and the effects of impoundment on OM moving through the river-reservoir system, are not well understood. Particulate organic carbon (POC), dissolved organic carbon (DOC), particulate nitrogen (PN), chlorophyll a (chl- a ), and δ 15 N–PN and δ 13 C–POC isotopic ratios were measured bi-weekly for over 2 years at four locations through the Snake River Hells Canyon Reservoir Complex to determine spatial and temporal patterns of OM quantities and sources. POC concentrations increased through the riverine zone upstream of the reservoirs, likely due to in situ primary production and/or inputs from tributaries and agricultural drains; then decreased through the most upstream reservoir likely due to particle settling. Isotopic ratios and other OM source indicators (δ 15 N–PN, δ 13 C–POC, POC:PN, chl- a :POC) show that the dominant source of particulate OM was phytoplankton with seasonal terrestrial/macrophytic inputs. Results highlight the effects of major tributary and agricultural drain inputs, primary production, and impoundment on OM composition and concentration through a large river-reservoir system and may inform water quality management efforts in this and similar systems.

Idaho, Oregon↗