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Low-cost field estimation of yellow perch daily ration

We used 2 yearsˈ diel food weight data from stomachs of Lake Erie yellow perch Perca flavescens to make Elliott‐Persson (E–P) estimates of daily ration and to construct consumption response surface (CRS) regression models. The CRS models provided relatively accurate, low‐cost estimates of daily ration from only one to four fish collections – compared with nine for the E–P procedure. Besides reducing daily sampling effort, CRS models allowed flexibility in fish collection times, thus alleviating the need for 24‐h associations with sampling sites. Independent variables for CRS models included the food weight in fish stomachs from a particular collection (adjusted for water temperature) and the time of day when a fish collection was made. Accuracy of CRS model estimates of yellow perch daily ration relative to E–P estimates was evaluated with a jackknife‐like procedure. Effectiveness of CRS models arose from significant relationships that existed between food weights in yellow perch stomachs throughout certain periods of the day and average food weight in stomachs over 24 h. Similar relationships, and hence low‐cost estimation potential, are expected for other fish species with regular diel feeding activity. Because CRS models reduce time requirements in the field, they should allow more detailed coverage of important spatial and temporal variability in multiday food consumption studies for fishes. The CRS models also showed spatial robustness and a desirable capacity to estimate daily rations in years beyond those for which the models were developed.

Transactions of the American Fisheries Society↗

Physiological response of largemouth bass to angling stress

The physiological effects of catch-and-release fishing on largemouth bass Micropterus salmoides from Lake Powell and Mantua Reservoir, Utah, were evaluated, and an estimate of the time needed for recovery from hooking stress was obtained. Fatigue in Lake Powell fish, as indicated by elevated blood lactate, was directly proportional to hooking time (1–5 min) and water temperature, but recovery from the hyperlacticemia was relatively rapid (about 24 h). Hyperglycemia, an indicator of stress hormone production, did not occur in largemouth bass hooked and played for 1–5 min in the coldest water (11–13°C), was moderate in fish hooked and played at l6–20°C, and was severe in fish played for 5 min at 28–30°C. Fish held for recovery in live cages suffered further hyperglycemia, presumably because of the stress of confinement. Ionoregulation, as indicated by relatively stable plasma chloride values, was not immediately affected in largemouth bass caught at water temperatures of 11–13°C or 28–30°C, but an unusual hyperchloremia developed in fish hooked and played at 16–20°C. During recovery, the expected progressive hypochloremia developed. Plasma osmolality was somewhat affected by hooking at all water temperatures tested, but recovery was almost complete within about 8 h. Mantua Reservoir fish were hooked and played only at water temperatures of 23–26°C. The hyperlacticemia and hyperglycemia that occurred were generally more severe than in the Lake Powell fish hooked and played at either 16–20°C or 28–30°C. However, effects on plasma chloride and osmolality were similar to those occurring in Lake Powell fish.

Transactions of the American Fisheries Society↗

Variation in feeding, aggression, and position choice between hatchery and wild cutthroat trout in an artificial stream

I compared feeding, aggressive behavior, and spatial distribution of differently ranked individuals of hatchery and wild coastal cutthroat trout Oncorhynchus clarki clarki in an artificial stream. Both hatchery and wild groups established stable dominance hierarchies that seemed to be based on size differences. Hatchery and wild fish within a hierarchical rank fed at similar rates. Hatchery fish were more aggressive than their wild conspecifics, irrespective of rank. Dominant hatchery fish were evenly distributed in pools and riffles, whereas dominant wild fish were three times more often in pools than in riffles. In both groups, socially intermediate fish were almost evenly distributed between pools and riffles, and subordinate fish spent most of their time in pools. On average, hatchery fish spent 57% of their time in pools and 43% in riffles, whereas wild fish spent 71% of their time in pools and 29% in riffles. These results support the hypothesis that excessive expenditure of energy for unnecessary aggression, use of fast-flowing water, or other purposes contributes to poor survival of hatchery fish after they are stocked in streams. Poor survival would reduce the efficacy of using hatchery stocks to supplement wild production.

Transactions of the American Fisheries Society↗

An intergeneric hybrid of a native minnow, the golden shiner, and an exotic minnow, the rudd

The hybrid golden shiner Notemigonus crysoleucas × rudd Scardinius erythrophthalmus is the first known nonsalmonid, intergeneric hybrid of an exotic species and a North American native species. The cross is also the first valid record of a viable hybrid involving the native golden shiner. Meristic and mensural characters of 30 artificially produced hybrids of male golden shiners and female rudds were analyzed. Forty-seven percent of the meristic traits exhibited character states intermediate between those of parents. Twenty-seven percent of the meristic characters were supernumerary, suggesting developmental instability of the hybrid genome. Mensural hybrid characters were significantly skewed to the golden shiner phenotype. The skewed mensural inheritance and other skewed patterns of morphological inheritance also suggest problems in canalization of the hybrid phenome or atypical patterns of dominance. All hybrids were identifiable by intermediate squamation of the cultrate abdomen: the keel was mostly scaled but exhibited a small fleshy ridge posteriorly. This minnow hybrid allows general inferences to be made about the phylogenetic affinity of the golden shiner to other cultrate cyprinids of Eurasia. The hybrid cross has important management and conservation implications for fishes in North America. The hybrid is an example of how an exotic species may negatively affect a native species.

Transactions of the American Fisheries Society↗

Toxicity of agricultural subsurface drainwater from the San Joaquin Valley, California to juvenile chinook salmon and striped bass

Juvenile chinook salmon Oncorhynchus tshawytscha (40-50 mm total length, TL) and striped bass Morone saxatilis (30-40 mm TL) were exposed to serial dilutions (100, 50, 25, and 12.5%) of agricultural subsurface drainwater (WWD), reconstituted drainwater (RWWD), and reconstituted seawater (IO). Agricultural subsurface drainwater contained naturally elevated concentrations of major ions (such as sodium and sulfate) and trace elements (especially boron and selenium), RWWD contained concentrations of major ions that mimicked those in WWD but trace elements were not elevated, and IO contained concentrations of total dissolved salt that were similar to those in WWD and RWWD but chloride replaced sulfate as the dominant anion. After 28 d of static exposure, over 75% of the chinook salmon in 100% WWD had died, whereas none had died in other dilutions and water types. Growth of chinook salmon in WWD and RWWD, but not in IO, exhibited dilution responses. All striped bass died in 100% WWD within 23 d, whereas 19 of 20 striped bass had died in 100% RWWD after 28 d. In contrast, none died in 100% IO. Growth of striped bass was impaired only in WWD. Fish in WWD accumulated as much as 200 μg/g (dry-weight basis) of boron, whereas fish in control water accumulated less than 3.1 μg/g. Although potentially toxic concentrations of selenium occurred in WWD (geometric means, 158-218 μg/L), chinook salmon and striped bass exposed to this water type accumulated 5.7 μg Se/g or less. These findings indicate that WWD was toxic to chinook salmon and striped bass. Judging from available data, the toxicity of WWD was due primarily to high concentrations of major ions present in atypical ratios, to high concentrations of sulfate, or to both. High concentrations of boron and selenium also may have contributed to the toxicity of WWD, but their effects were not clearly delineated.

California↗

Genetic variation in steelhead of Oregon and northern California

Steelhead Oncorhynchus mykiss from various sites between the Columbia River and the Mad River, California, were genetically characterized at 10 protein-coding loci or pairs of loci by starch gel electrophoresis. Fish from coastal streams differed from fish east of the Cascade Mountains and from fish of the Willamette River (a tributary of the Columbia River, west of the Cascade Mountains). Coastal steelhead from the northern part of the study area differed from those in the southern part. Genetic differentiation within and among drainages was not statistically significant; however, gene diversity analysis and the life history of steelhead suggested that fish from different drainages should be considered as separate populations. Genetic variation among fish in separate drainages was similar to that reported in northwestern Washington and less than that reported in British Columbia. Allele frequencies varied significantly among year-classes. Genetic variation within samples accounted for 98.3% of the total genetic variation observed in this study. Most hatchery populations differed from wild populations, suggesting that conservation of genetic diversity among and within wild populations could be facilitated by altering hatchery programs.

Transactions of the American Fisheries Society↗

Performance of nine external tags on hatchery-reared rainbow trout

We evaluated nine commercially available tags to determine their suitability for marking yearling rainbow trout Oncorhynchus mykiss reared in raceways and circular tanks. The tags tested were Floy vinyl tubing tags FD‐67 anchor, FT‐2 dart, FT‐4 cinch‐up, and FT‐4 lock‐on; modified Carlin; modified Petersen disk; Monel strap 4‐1005; Monel butt end 4‐1242; and Stoffel fish seal. After 9 months, tag losses from raceway fish were lowest (8%) for the FT‐4 lock‐on tag; in tanks, none of the FT‐2 dart, FT‐4 cinch‐up, FT‐4 lock‐on, or the Monel butt end tags were lost. At 90 d after tagging, raceway fish marked with the Monel strap or the modified Carlin tags showed significantly less growth than did fish marked with the FD‐67 anchor tag; the converse was true for fish in tanks. After 9 months, raceway fish marked with the Monel butt end tag and tank fish marked with the FD‐67 anchor tag were least injured. Injury was greater in raceways than in tanks for all tag types. Judged by overall performance, the FD‐67 anchor was the most effective tag for fish maintained in raceways and the FT‐4 cinch‐up was a suitable alternative; the FT‐2 dart, FT‐4 cinch‐up, and Monel butt end tags were equally suitable for fish in tank culture.

Transactions of the American Fisheries Society↗

Notes: Group-size-mediated metabolic rate reduction in American shad

The relation of oxygen consumption to ambient temperature and group size was studied in juvenile American shad Alosa sapidissima at three group sizes: 5 or 6 (small), 9–13 (medium), and 41–48 (large) fish per 500‐L tank. Oxygen consumption rates, water temperatures, and behavior were measured concurrently in the three group‐size treatments. Schooling predominated in large groups, whereas aggregating behavior dominated medium and small groups. Fish in small or medium groups consumed oxygen at two to four times the rate of fish in large groups (0.36 or 0.25 versus 0.11 mg O 2 /[g biomass·h]). This group‐size‐related respiratory rate reduction could be caused by the effect of calming, type of swimming, swimming speed, or hydrodynamic advantage.

Transactions of the American Fisheries Society↗

Larval American shad: Effects of age and group size on swimming and feeding behavior

We analyzed the behavior of 3–4‐d‐old prolarval and 28–33‐d‐old metalarval American shad Alosa sapidissima in groups of 3–1,000 fish per 22‐L glass tank, to determine whether (1) previously described juvenile behavior patterns first develop in larvae, (2) group size or density alters the behavior of larvae, and (3) schooling or other forms of cohesive behavior develop in larvae to promote social interactions. Twelve discrete behaviors or modal action patterns (MAPS) oflarvae were observed at all group sizes; halfthese patterns are unique to larval stages. Conversely, larvae do not develop five previously described juvenile MAPS. Stereotyped metalarval feeding sequences were absent or poorly developed in prolarvae. Group size was directly related to duration of free swimming in water column (metalarvae only) and to frequencies of “proximity to another fish” (all larvae), “contact another fish” (all larvae), and “escape or flee” (all larvae). Age or larval stage significantly affected all swimming‐related activities and three feeding behaviors. Larvae foraged and fed independently of one another and used MAPs typical of other larval fishes (“fixate,” “sigmoid,” “lunge,” and “capture”). With one exception (a direct relationship between frequency of food capture and metalarval size), group size and individual size did not significantly affect larval feeding success. Neither schooling nor forms of behavior leading to coordinated group swimming were observed at either larval stage. Larval behavior differed from juvenile behavior in a way that suggests survival in riverine habitats is promoted by behavior that disperses larvae and enables them to function nonsocially.

Transactions of the American Fisheries Society↗

Predation by northern squawfish on live and dead juvenile Chinook salmon

Northern squafish Ptychocheilus oregonensis is a major predator of juvenile salmonids Oncorhynchus spp. migrating downstream through the Columbia River. High predation rates occur just below dams. If northern squafish selectively consume salmonids killed or injured during dam passage, previous estimates of predation mortality may be too high. We conducted laboratory experiments that indicate northern squafish prefer dead juvenile chinook salmon O. tshawytscha over live individuals. When equal numbers of dead and live chinook salmon were offered to northern squafish maintained on a natural photoperiod (15 h light: 9 h darkness), significantly more ( P < 0.05) dead than live fish were consumed, both in 1,400-L circular tanks and in an 11,300-L raceway (62% and 79% of prey consumed were dead, respectively). When dead and live juvenile chinook salmon were provided in proportions more similar to those below dams (20% dead, 80% live), northern squafish still selected for dead prey (36% of fish consumed were dead). In additional experiments, northern squafish were offered a proportion of 20% dead juvenile chinook salmon during 4-h periods of either light or darkness. The predators were much more selective for dead chinook salmon during bright light (88% of fish consumed were dead) than during darkness (31% were dead).

Transactions of the American Fisheries Society↗

Development of a practical diet for juvenile striped bass

We formulated four diets that simulated the amino acid profiles of eggs of striped bass Morone saxatilis , carcasses of larval striped bass, eggs of rainbow trout Oncorhynchus mykiss , or nauplii of Artemia salina ; a fifth diet contained a high concentration (33.6%) of shrimp meal. These diets were fed to juvenile striped bass for 14 weeks. Striped bass grew best when fed diets with an amino acid pattern that simulated the patterns of rainbow trout eggs or Artemia salina . Fish fed these two diets grew as well as, and had carcass compositions and protein efficiency ratios similar to those of, control fish fed the federally specified ASD2‐30 salmon diet. No histological or morphological abnormalities were noted. These two diets, which have potentially lower production costs and contain slightly less protein than ASD2‐30, may be used as prototype diets specifically for striped bass. Through further minor refinements, the diets could be used in production hatcheries.

Transactions of the American Fisheries Society↗

Spawning and rearing habitat use by white sturgeons in the Columbia River downstream from McNary Dam

Spawning and rearing habitats used by white sturgeons Acipenser transmontanus were described from water temperature, depth, and velocity measurements and substrate types present at sites where eggs, larvae, young-of-the-year, and juveniles (ages 1–7) were collected. Spawning and egg incubation occurred in the swiftest water available (mean water column velocity, 0.8–2.8 m/s), which was within 8 km downstream from each of the four main-stem Columbia River dams in our study area. Substrates where spawning occurred were mainly cobble, boulder, and bedrock. Yolk-sac larvae were transported by the river currents from spawning areas into deeper areas with lower water velocities and finer substrates. Young-of-the-year white sturgeons were found at depths of 9–57 m, at mean water column velocities of 0.6 m/s and less, and over substrates of hard clay, mud and silt, sand, gravel, and cobble. Juvenile fish were found at depths of 2–58 m, at mean water column velocities of 1.2 m/s and less, and over substrates of hard clay, mud and silt, sand, gravel, cobble, boulder, and bedrock.

Transactions of the American Fisheries Society↗

Gamete ripening and hormonal correlates in three strains of lake trout

In our 2-year laboratory study of hatchery-reared adult lake trout Salvelinus namaycush of the Seneca Lake, Marquette (Lake Superior Lean), and Jenny Lake strains, we compared gamete ripening times and changes in plasma concentrations of seven hormones. If interstrain differences in these traits were found, such differences might help explain the apparent failure of stocked fish of these strains to develop large, naturally reproducing populations in the Great Lakes. The complex temporal changes in plasma hormone levels that occur during sexual maturation in lake trout have not been previously described. We detected little evidence of temporal isolation that would prevent interbreeding among the three strains. Strain had no effect on ovulation date (OD) in either year. Strain did not affect spermiation onset date (SOD) in year 1 but did in year 2, when the mean SOD of Jenny Lake males was earlier than that of Seneca Lake males but not different from that of Marquette males. Hormonal data were normalized around ODs for individual females and SODs for individual males. In females, estradiol-17&beta; (E2) was highest 8 weeks before the OD; the highest testosterone (T) level occurred 6 weeks before the OD, and the next highest level occurred simultaneously with the highest level of 11-ketotestosterone (11-KT) 2 weeks before the OD. Plasma levels of 17&prop;-hydroxy-20&beta;-dihydroprogesterone (DHP) peaked 1 week before the OD, then abruptly declined immediately after. Cortisol (F), triiodothyronine (T 3 ), and thyroxine (T 4 ) were highly variable, but F was the only hormone that showed no trend with week in either year. In males, plasma E2 levels were highest 3 weeks before the SOD, highest levels of T and of 11-KT occurred simultaneously 2 weeks after the SOD, and DHP peaked 5 weeks after the SOD and 3 weeks after the highest levels of T and 11-KT. As in females, plasma levels of F, T 3 , and T 4 were highly variable, and F was the only hormone that showed no trend with week in either year. Strain had no effect on any hormones in females and only on T and F in males. The lack of pronounced interstrain differences in gamete ripening dates and reproductive endocrinology and the similarity of the temporal patterns and relative concentrations of hormones to those reported for other salmonids suggest nothing unusual or dysfunctional about these reproductive traits that would impede lake trout rehabilitation in the Great Lakes.

Transactions of the American Fisheries Society↗

Factors affecting feeding behavior and survival of juvenile lake trout in the Great Lakes

We explored the importance of experience with feeding on live prey, of cataracts, of strain, and of maternally transferred contaminants for the feeding rate and predator avoidance behavior of young lake trout Salvelinus namaycush . Hatchery-reared and feral juvenile lake trout were tested separately as predators on lake trout fry in tanks with artificial cobble reefs. Feral fish captured more prey per day and more prey per strike than did hatchery lake trout. The predatory performance of hatchery and feral fish did not improve significantly with experience. Feeding rates did not differ between lake trout with unilateral cataracts and normal-eyed fish, but significantly diminished for lake trout with bilateral cataracts. Neither strain nor contaminant background affected the ability of fry to feed or to avoid predators. Of the factors studied, previous experience with live food under natural conditions (i.e., the experience of feral fish) was the most important factor affecting feeding behavior of young lake trout.

Transactions of the American Fisheries Society↗

Simulation of the effects of time and size at stocking on PCB accumulation in lake trout

Manipulations of size at stocking and timing of stocking have already been used to improve survival of stocked salmonines in the Great Lakes. It should be possible to stock salmonines into the Great Lakes in a way that reduces the rate of polychlorinated biphenyl (PCB) accumulation in these fishes. An individual-based model (IBM) was used to investigate the effects of size at stocking and timing of stocking on PCB accumulation by lake trout Salvelinus namaycush in Lake Michigan. The individual-based feature of the model allowed lake trout individuals to encounter prey fish individuals and then consume sufficiently small prey fish. The IBM accurately accounted for the variation in PCB concentrations observed within the Lake Michigan lake trout population. Results of the IBM simulations revealed that increasing the average size at stocking from 110 to 160 mm total length led to an increase in the average PCB concentration in the stocked cohort at age 5, after the fish had spent 4 years in the lake, from 2.33 to 2.65 mg/kg; the percentage of lake trout in the cohort at the end of the simulated time period with PCB concentration of 2 mg/kg or more increased from 62% to 79%. Thus, PCB contamination was reduced when the simulated size at stocking was smallest. An overall stocking strategy for lake trout into Lake Michigan should weigh this advantage regarding PCB contamination against the poor survival of lake trout that may occur if the trout are stocked at too small a size.

Transactions of the American Fisheries Society↗

Distribution, abundance, and resting microhabitat of burbot on Julian's Reef, southwestern Lake Michigan

We used a remotely operated submersible vehicle equipped with a color video camera to videotape the lake bed and document the distribution and abundance of burbot Lota lota on a 156‐hectare portion of Julianˈs Reef in southwestern Lake Michigan. The substrates and bathymetry of the study area had been mapped recently by side‐scan sonar. Burbot density determined from videotapes covering 6,900 m 2 of lake bed at depths of 23–41 m averaged 139 individuals/ hectare (range, 0–571/hectare). This density was substantially higher than the highest burbot density (59–95/hectare) reported in the literature. Burbot were present on the lake bed at depths of 23–36 m, but were most abundant near the crest of the reef at 23–28 m, where the water temperature was 8–13°C, their preferred summer temperature range. Substrates in that temperature range on the reef were bedrock, bedrock ridges, and bedrock and rubble. Burbot were most abundant on the bedrock and rubble. Small fish and macroinvertebrates typically eaten by burbot elsewhere in western Lake Michigan were distributed on the reef according to their summer preferred temperatures and were not seen in abundance where burbot density was highest. We saw no lake trout Salvelinus namaycush on Julianˈs Reef, although large numbers of juvenile lake trout have been stocked there annually and temperatures on the reef were in the preferred summer temperature range for lake trout.

Michigan, Wisconsin↗

Interaction between native and nonnative fish of the upper Muddy River, Nevada

I investigated interactions between native and nonnative fishes in the upper Muddy River system to add insight into (1) the mechanism causing the decline of the Moapa dace Moapa coriacea after the introduction of the shortfin molly Poecilia mexicana , (2) the reason Moapa White River springfish Crenichthys bailevi moapae were less affected by the introduction, and (3) the reason interactions between natives is relatively benign. I investigated the hypothesis that the shortfin molly caused the decline of the Moapa dace through competition or predation on larvae, pressures not experienced by the Moapa White River springfish. Relative interspecific competition was analyzed by contrasting the ranges of spatial and dietary overlap among larval, juvenile, and adult life stages. There appeared to be moderate to low spatial overlap between the various life stages of native and nonnative fishes. Overlap in diet was highest between adult Moapa White River springfish and shortfin mollies. Laboratory experiments suggested that shortfin mollies prey vigorously upon fish larvae. In terms of spatial habitat use, Moapa White River springfish larvae were less available to adult shortfin mollies for consumption than were Moapa dace larvae. When predation on larvae is the mechanism by which nonnative fish reduce native forms, aggressiveness of the predator and the degree to which the predator overlaps in habitat with the prey may influence the degree to which a native fish population is affected.

Transactions of the American Fisheries Society↗

Sustainability of hatchery-dependent salmonine fisheries in Lake Ontario: The conflict between predator demand and predator supply

The offshore fish community of Lake Ontario is presently dominated by intensively managed, nonnative species: Alewife Alosa pseudoharengus and rainbow smelt Osmerus mordax at the planktivore level and stocked salmonines at the piscivore level. Salmonine stocking rates per unit area of Lake Ontario are the highest in the Great Lakes, and fishery managers are concerned about the sustainability of the fishery under present stocking policies, particularly with the recent collapse of the Lake Michigan fishery for chinook salmon Oncorhynchus tshawytscha . In this paper, we describe and present the results of a simulation model that integrates predator demand estimates derived from bioenergetics, prey and predator population dynamics, and a predation model based on the multiple-species functional response, Model reconstructions of historical alewife biomass trends and salmonine diets corresponded reasonably well with existing data for the period 1978&ndash;1992. The simulations suggest that current predator demand does not exceed the threshold beyond which alewife biomass cannot be sustained, but they indicate that the sustainability of the prey fish community is extremely sensitive to fluctuations in overwinter survival of alewife; an additional mortality of 25% in a single winter would be sufficient to cause the collapse of the alewife population. The model includes a number of assumptions and simplifications with a limited empirical basis; better estimates of salmonine survival rates, an evaluation of the importance of spatial and temporal interactions among predators and prey, and incorporation of the effects of recently observed declines in system productivity at lower trophic levels would significantly increase confidence in the model's projections.

Transactions of the American Fisheries Society↗