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At least 613 records · Page 34Linked to original sources

Direct measurements of copper speciation in basaltic glasses: Understanding the relative roles of sulfur and oxygen in copper complexation in melts

Micro-analytical determination of copper (Cu) speciation in natural magmatic glasses, equilibrated below the nickel – nickel oxide (NNO) buffer, reveals that two copper species are commonly stabilized in such basaltic melts. X-ray absorption fine structure (XAFS) spectroscopic analysis of basaltic matrix glasses and melt inclusions (MI) from samples of mid-ocean ridge basalt (MORB), and from Nyamuragira, Etna and Kīlauea volcanoes show that both Cu(I)-sulfide and Cu(I)-oxide species are stabilized. The proportion of each species correlates with the measured sulfur (S) abundance of the glass. In glasses with S abundances greater than ~1000 ppm, Cu(I)-sulfide species are dominant, whereas in glasses with S abundances between 500 and 1000 ppm, both species are found to coexist. The Cu(I)-oxide species dominate at S concentrations below 500 ppm. In 1 atm S-free experimental glasses of basaltic composition that we analyzed, only Cu(I)-oxide species are detectable, regardless of the oxygen fugacity (fO2), even at relatively high fO2 values well above the NNO buffer. Our results demonstrate that XAFS techniques are highly sensitive in measuring Cu speciation in reduced (below NNO) basaltic glasses and that both oxide and sulfide complexes can be stabilized. The relative proportion of these two species is highly dependent on the concentration of S in the melt, and thus the Cu speciation in natural melts changes as S is lost from the melt by low pressure degassing.

Hawaii↗

Modeling hexavalent chromium reduction in groundwater in field-scale transport and laboratory batch experiments

A plausible and consistent model is developed to obtain a quantitative description of the gradual disappearance of hexavalent chromium (Cr(VI)) from groundwater in a small-scale field tracer test and in batch kinetic experiments using aquifer sediments under similar chemical conditions. The data exhibit three distinct timescales. Fast reduction occurs in well-stirred batch reactors in times much less than 1 hour and is followed by slow reduction over a timescale of the order of 2 days. In the field, reduction occurs on a timescale of the order of 8 days. The model is based on the following hypotheses. The chemical reduction reaction occurs very fast, and the longer timescales are caused by diffusion resistance. Diffusion into the secondary porosity of grains causes the apparent slow reduction rate in batch experiments. In the model of the field experiments, the reducing agent, heavy Fe(II)-bearing minerals, is heterogeneously distributed in thin strata located between larger nonreducing sand lenses that comprise the bulk of the aquifer solids. It is found that reducing strata of the order of centimeters thick are sufficient to contribute enough diffusion resistance to cause the observed longest timescale in the field. A one-dimensional advection/dispersion model is formulated that describes the major experimental trends. Diffusion rates are estimated in terms of an elementary physical picture of flow through a stratified medium containing identically sized spherical grains. Both reduction and sorption reactions are included. Batch simulation results are sensitive to the fraction of reductant located at or near the surface of grains, which controls the amount of rapid reduction, and the secondary porosity, which controls the rate of slow reduction observed in batch experiments. Results of Cr(VI) transport simulations are sensitive to the thickness and relative size of the reducing stratum. Transport simulation results suggest that nearly all of the reductant must be located in the reducing stratum. Within this context and as long as there is adequate reductive capacity present, the transport simulation results are insensitive to the parameters important for the batch simulations. The results illustrate how a combination of field measurements and batch laboratory studies can be used to improve predictive modeling of contaminant transport.

Water Resources Research↗

Widespread occurrence of former anhydrite phenocrysts in Laramide-age magmas related to porphyry-skarn Cu mineralization at Santa Rita and Hanover-Fierro, New Mexico, USA

Reports of magmatic anhydrite are relatively rare, with only ~30 occurrences documented worldwide so far. However, magmatic anhydrite saturation is difficult to recognize because anhydrite decomposes rapidly in near-surface environments. In most cases, only anhydrite inclusions shielded within other phenocryst phases were able to survive. Alternatively, since anhydrite phenocrysts preserved in fresh volcanic rocks are characteristically intergrown with apatite phenocrysts, the former presence of anhydrite phenocrysts can be recognized based on the occurrence of lath-shaped cavities that show a strong spatial association with apatite phenocrysts. These cavities can be either empty or filled with low-temperature, secondary minerals such as zeolites, carbonates, or microcrystalline silica. A systematic search for the occurrence of such cavities, combined with optical and Raman-spectroscopic identification of anhydrite inclusions preserved within apatite, hornblende and quartz phenocrysts, demonstrates that most of the Laramide-age magmas associated with the Santa Rita and Hanover-Fierro porphyry-skarn Cu (Zn, Mo, Au, Pb) deposits were saturated in magmatic anhydrite. The anhydrite typically coexisted with monosulfide solid solution (MSS), suggesting oxygen fugacities of ~2.0±0.5 log units above the fayalite-magnetite-quartz buffer. The magmas range from andesitic to rhyodacitic in composition, and from shortly pre-mineralization (~61 Ma) to shortly post-mineralization (~57 Ma) in age. In three samples with particularly well-recognizable former anhydrite phenocrysts, their modal abundance could be quantified based on high-resolution scans of polished hand specimens. The observed modal anhydrite abundances of 0.63–1.8 vol% translate into minimum magma sulfur contents of 0.20–0.56 wt% S. The highest sulfur content of 0.56 wt% S is difficult to reconcile with available anhydrite solubility models, but it could be reproduced in an anhydrite solubility experiment performed at 950 °C and 1.15 GPa on a natural latite containing 13.1 wt% dissolved H2O. The sample with the second-highest sulfur content of 0.26 wt% S requires ~10 wt% H2O in the silicate melt, and, consequently, a minimum pressure of ~0.5 GPa. Taken together, the results suggest that the magmas of the Central Mining District were extremely hydrous and thus originated from great depth. Indeed, their major element compositions and reconstructed H2O and S contents agree well with experimentally observed and numerically predicted compositions of residual silicate melts after 50–70 wt% crystallization of ordinary arc basalts at high pressure and high oxygen fugacities.

New Mexico↗

Avian influenza in shorebirds: experimental infection of ruddy turnstones (Arenaria interpres) with avian influenza virus

Background: Low pathogenic avian influenza viruses (LPAIV) have been reported in shorebirds, especially at Delaware Bay, USA, during spring migration. However, data on patterns of virus excretion, minimal infectious doses, and clinical outcome are lacking. The ruddy turnstone (Arenaria interpres) is the shorebird species with the highest prevalence of influenza virus at Delaware Bay. Objectives: The primary objective of this study was to experimentally assess the patterns of influenza virus excretion, minimal infectious doses, and clinical outcome in ruddy turnstones. Methods: We experimentally challenged ruddy turnstones using a common LPAIV shorebird isolate, an LPAIV waterfowl isolate, or a highly pathogenic H5N1 avian influenza virus. Cloacal and oral swabs and sera were analyzed from each bird. Results: Most ruddy turnstones had pre-existing antibodies to avian influenza virus, and many were infected at the time of capture. The infectious doses for each challenge virus were similar (103·6–104·16 EID50), regardless of exposure history. All infected birds excreted similar amounts of virus and showed no clinical signs of disease or mortality. Influenza A-specific antibodies remained detectable for at least 2 months after inoculation. Conclusions: These results provide a reference for interpretation of surveillance data, modeling, and predicting the risks of avian influenza transmission and movement in these important hosts.

Influenza and Other Respiratory Viruses↗

A missing dimension in measures of vaccination impacts

Immunological protection, acquired from either natural infection or vaccination, varies among hosts, reflecting underlying biological variation and affecting population-level protection. Owing to the nature of resistance mechanisms, distributions of susceptibility and protection entangle with pathogen dose in a way that can be decoupled by adequately representing the dose dimension. Any infectious processes must depend in some fashion on dose, and empirical evidence exists for an effect of exposure dose on the probability of transmission to mumps-vaccinated hosts [1] , the case-fatality ratio of measles [2] , and the probability of infection and, given infection, of symptoms in cholera [3] . Extreme distributions of vaccine protection have been termed leaky (partially protects all hosts) and all-or-nothing (totally protects a proportion of hosts) [4] . These distributions can be distinguished in vaccine field trials from the time dependence of infections [5] . Frailty mixing models have also been proposed to estimate the distribution of protection from time to event data [6] , [7] , although the results are not comparable across regions unless there is explicit control for baseline transmission [8] . Distributions of host susceptibility and acquired protection can be estimated from dose-response data generated under controlled experimental conditions [9] – [11] and natural settings [12] , [13] . These distributions can guide research on mechanisms of protection, as well as enable model validity across the entire range of transmission intensities. We argue for a shift to a dose-dimension paradigm in infectious disease science and community health.

PLoS Pathogens↗

Experimental warming changes phenology and shortens growing season of the dominant invasive plant Bromus tectorum (cheatgrass)

Bromus tectorum (cheatgrass) has successfully invaded and established throughout the western United States. Bromus tectorum grows early in the season and this early growth allows B. tectorum to outcompete native species, which has led to dramatic shifts in ecosystem function and plant community composition after B. tectorum invades. If the phenology of native species is unable to track changing climate as effectively as B. tectorum ’s phenology then climate change may facilitate further invasion. To better understand how B. tectorum phenology will respond to future climate, we tracked the timing of B. tectorum germination, flowering, and senescence over a decade in three in situ climate manipulation experiments with treatments that increased temperatures (2°C and 4°C above ambient), altered precipitation regimes, or applied a combination of each. Linear mixed-effects models were used to analyze treatment effects on the timing of germination, flowering, senescence, and on the length of the vegetative growing season (time from germination to flowering) in each experiment. Altered precipitation treatments were only applied in early years of the study and neither precipitation treatments nor the treatments’ legacies significantly affected B. tectorum phenology. The timing of germination did not significantly vary between any warming treatments and their respective ambient plots. However, plots that were warmed had advances in the timing of B. tectorum flowering and senescence, as well as shorter vegetative growing seasons. The phenological advances caused by warming increased with increasing degrees of experimental warming. The greatest differences between warmed and ambient plots were seen in the length of the vegetative growing season, which was shortened by approximately 12 and 7 days in the +4°C and +2°C warming levels, respectively. The effects of experimental warming were small compared to the effects of interannual climate variation, suggesting that interactive controls and the timing of multiple climatic factors are important in determining B. tectorum phenology. Taken together, these results help elucidate how B. tectorum phenology may respond to future climate, increasing our predictive capacity for estimating when to time B. tectorum control efforts and how to more effectively manage this exotic annual grass.

Utah↗

Virulence and infectivity of UC, MD and L strains of infectious hematopoietic necrosis virus (IHNV) in four populations of Columbia River Basin Chinook salmon

Infectious Hematopoietic Necrosis Virus (IHNV) infects juvenile salmonid fish in conservation hatcheries and aquaculture facilities, and in some cases, causes lethal disease. This study assesses intra-specific variation in the IHNV susceptibility of Chinook salmon ( Oncorhynchus tshawytscha ) in the Columbia River Basin (CRB), in the northwestern United States. The virulence and infectivity of IHNV strains from three divergent virus genogroups are measured in four Chinook salmon populations, including spring-run and fall-run fish from the lower or upper regions of the CRB. Following controlled laboratory exposures, our results show that the positive control L strain had significantly higher virulence, and the UC and MD strains that predominate in the CRB had equivalently low virulence, consistent with field observations. By several experimental measures, there was little variation in host susceptibility to infection or disease. However, a small number of exceptions suggested that the lower CRB spring-run Chinook salmon population may be less susceptible than other populations tested. The UC and MD viruses did not differ in infectivity, indicating that the observed asymmetric field prevalence in which IHNV detected in CRB Chinook salmon is 83% UC and 17% MD is not due to the UC virus being more infectious. Overall, we report little intra-species variation in CRB Chinook salmon susceptibility to UC or MD IHNV infection or disease, and suggest that other factors may instead influence the ecology of IHNV in the CRB.

Columbia River Basin↗

U.S. Geological Survey Grand Canyon Monitoring and Research Center: Proceedings of the fiscal year 2023 annual reporting meeting to the Glen Canyon Dam Adaptive Management Program

This proceedings is prepared for the USBR and Glen Canyon Dam Adaptive Management Program (GCDAMP) to account for work conducted and products delivered in FY 2023 by SBSC's Grand Canyon Monitoring and Research Center (GCMRC) and to inform the Technical Work Group of science conducted by GCMRC and its cooperators in support of the GCDAMP. It includes a summary of accomplishments, modifications to work plans, and results related to projects included in GCMRC’s FY 2021-23 Triennial Work Plan. This work was done to support the 11 resource goals identified in the Glen Canyon Dam Long-Term Experimental and Management Plan (LTEMP) Environmental Impact Statement and Record of Decision.

Arizona, Nevada, Utah↗

α-Radioactivity of cerium-142

JOHNSON AND NIER 1 have measured the atomic masses of some of the rare-earth isotopes and have shown that the mass difference cerium-142—(barium-138 + helium-4) is equivalent to 1.68 ± 0.10 MeV. Similar results for the naturally occurring samarium and neodymium isotopes show that the α -active isotope of each element is the one having the largest possible decay energy. Rasmussen and others 2 suggest that the two or three neutrons just beyond the closed shell of 82 neutrons have decreased binding energies and hence the α -energy has a maximum about 84 neutrons. Johnson and Nier suggest that the α -decay of cerium-142 may take place with enough energy to be experimentally observable. Porschen and Riezler 3 examined a sample of un-enriched cerium ammonium citrate using nuclear track plates sensitive to α -particles. No α -activity was observed after a 30-day exposure of 1.2 mgm. of the cerium salt. In 1957 Riezler and Kauw 4 reported an alpha activity for an enriched sample of cerium-142. From their results they calculated a half-life of 5.1 × 10 15 years with an uncertainty factor of 2.

Nature↗

USGS Workshop on Scientific Aspects of a Long-Term Experimental Plan for Glen Canyon Dam, April 10-11, 2007, Flagstaff, Arizona

Executive Summary Glen Canyon Dam is located in the lower reaches of Glen Canyon National Recreation Area on the Colorado River, approximately 15 miles upriver from Grand Canyon National Park (fig. 1). In 1992, Congress passed and the President signed into law the Grand Canyon Protection Act (GCPA; title XVIII, sec. 1801?1809, of Public Law 102-575), which seeks ?to protect, mitigate adverse impacts to, and improve the values for which Grand Canyon National Park and Glen Canyon National Recreation Area were established.? The Glen Canyon Dam Adaptive Management Program (GCDAMP) was implemented as a result of the 1996 Record of Decision on the Operation of Glen Canyon Dam Final Environmental Impact Statement to ensure that the primary mandate of the GCPA is met through advances in information and resources management (U.S. Department of the Interior, 1995). On November 3, 2006, the Bureau of Reclamation (Reclamation) announced it would develop a long-term experimental plan environmental impact statement (LTEP EIS) for operational activities at Glen Canyon Dam and other management actions on the Colorado River. The purpose of the long-term experimental plan is twofold: (1) to increase the scientific understanding of the ecosystem and (2) to improve and protect important downstream resources. The proposed plan would implement a structured, longterm program of experimentation to include dam operations, potential modifications to Glen Canyon Dam intake structures, and other management actions such as removal of nonnative fish species. The development of the long-term experimental plan continues efforts begun by the GCDAMP to protect resources downstream of Glen Canyon Dam, including Grand Canyon, through adaptive management and scientific experimentation. The LTEP EIS will rely on the extensive scientific studies that have been undertaken as part of the adaptive management program by the U.S. Geological Survey?s (USGS) Grand Canyon Monitoring and Research Center (GCMRC), one of the four research stations within the USGS Southwest Biological Science Center. On April 10 and 11, 2007, at the behest of Reclamation, the GCMRC convened a workshop with scientific experts to identify one or more scientifically credible, long-term experimental options for Reclamation to consider for the LTEP EIS that would be consistent with the purpose and need for the plan. Workshop participants included government, academic, and private scientists with broad experience in the Colorado River in Grand Canyon and regulated rivers around the world. Resource managers and GCDAMP participants were also present on the second day of the workshop. In advance of the workshop, Reclamation and LTEP EIS cooperating agencies identified 14 core scientific questions. Workshop participants were asked to consider how proposed options would address these questions, which fall primarily into four areas: (1) conservation of endangered humpback chub (Gila cypha) and other high-priority biological resources, (2) conservation of sediment resources, (3) enhancement of recreational resources, and (4) preservation of cultural resources. A secondary objective of the workshop was the evaluation of four long-term experimental options developed by the GCDAMP Science Planning Group (SPG) (appendix B). The flow and nonflow treatments called for in the four experimental options were an important starting point for workshop discussions. At the beginning of the workshop, participants were provided with the final LTEP EIS scoping report prepared by Reclamation. Participants were also advised that Reclamation had committed to ?make every effortEto ensure that a new population of humpback chub is established in the mainstem or one or more of the tributaries within Grand Canyon? in the 1995 Operation of Glen Canyon Dam Final Environmental Impact Statement (U.S. Department of the Interior, 1995). This decision was consistent with the U.S. Fish and Wildlife Service?s 1995 bi

Open-File Report↗

Determination of nitrogen in coal macerals using electron microprobe technique-experimental procedure

This paper discusses nitrogen determination with the Cameca SX50 electron microprobe using PCO as an analyzing crystal. A set of conditions using differing accelerating voltages, beam currents, beam sizes, and counting times were tested to determine parameters that would give the most reliable nitrogen determination. The results suggest that, for the instrumentation used, 10 kV, current 20 nA, and a counting time of 20 s provides the most reliable nitrogen determination, with a much lower detection limit than the typical concentration of this element in coal. The study demonstrates that the electron microprobe technique can be used to determine the nitrogen content of coal macerals successfully and accurately. ?? 2001 Elsevier Science B.V. All rights reserved.

International Journal of Coal Geology↗

Fitness homeostasis across an experimental water gradient predicts species' geographic range and climatic breadth

Species range sizes and realized niche breadths vary tremendously. Understanding the source of this variation has been a long-term aim in evolutionary ecology and is a major tool in efforts to ameliorate the impacts of changing climates on species distributions. Species ranges that span a large climatic envelope can be achieved by a collection of specialized genotypes locally adapted to a small range of conditions, by genotypes with stable fitness across variable environments, or a combination of these factors. We asked whether fitness expressed along a key niche axis, water availability, could explain a species' realized niche breadth, its geographic range and climate breadth, in 11 species from a clade of jewelflowers whose range sizes vary by two orders of magnitude. Specifically, we explored whether the range size of a species was related to the ability of genotypes (maternal families) to maintain fitness across a range of experimental water availabilities based on 30-year historical field precipitation regimes. We operationally characterized fitness homeostasis through the coefficient of variation in fitness of a genotype (family) across the experimental water gradient. We found that species with genotypes that had high fitness homeostasis, low variation in fitness over our treatments, had larger climatic niche breadth and geographic range in their field distributions. The result was robust to alternate measures of fitness homeostasis. Our results show that the fitness homeostasis of genotypes can be a major factor contributing to niche breadth and range size in this clade. Fitness homeostasis can buffer species from loss of genetic diversity and under changing climates, provides time for adaptation to future conditions.

Ecology↗

Effects of behavioral and morphological plasticity on risk of predation in a Neotropical tadpole

Predator-induced phenotypic plasticity is widespread among aquatic animals, however the relative contributions of behavioral and morphological shifts to reducing risk of predation remain uncertain. We tested the phenotypic plasticity of a Neotropical tadpole (Rana palmipes) in response to chemical cues from predatory Belostoma water bugs, and how phenotype affects risk of predation. Behavior, morphology, and pigmentation all were plastic, resulting in a predator-induced phenotype with lower activity, deeper tail fin and muscle, and darker pigmentation. Tadpoles in the predator cue treatment also grew more rapidly, possibly as a result of the nutrient subsidy from feeding the caged predator. For comparison to phenotypes induced in the experiment, we quantified the phenotype of tadpoles from a natural pool. Wildcaught tadpoles did not match either experimentally induced phenotype; their morphology was more similar to that produced in the control treatment, but their low swimming activity was similar to that induced by predator cues. Exposure of tadpoles from both experimental treatments and the natural pool to a free-ranging predator confirmed that predator-induced phenotypic plasticity reduces risk of predation. Risk of predation was comparable among wild-caught and predator-induced tadpoles, indicating that behavioral shifts can substantially alleviate risk in tadpoles that lack the typical suite of predator-induced morphological traits. The morphology observed in wild-caught tadpoles is associated with rapid growth and high competition in other tadpole species, suggesting that tadpoles may profitably combine a morphology suited to competition for food with behaviors that minimize risk of predation. ?? Springer-Verlag 2004.

Oecologia↗

The effects of estradiol-17β on the sex reversal, survival, and growth of green sunfish Lepomis cyanellus

The feminization of green sunfish Lepomis cyanellus could expand their utility as a game fish or aquacultured species by preventing overcrowding and precocious reproduction in stocked systems. Feminization of green sunfish could also help elucidate information on their sex determination system. We report the feminization of green sunfish cohorts via oral administration of estradiol-17β (E2) during early development. A low-dose (100 E2 mg per kg of diet) and a high-dose (150 E2 mg per kg of diet) experimental E2 treatment were fed to juvenile green sunfish from 30 to 90 days post-hatch. Fish were subsequently evaluated for any treatment effect on gonadal development, survival, and growth. Both E2 treatments resulted in 100% feminization, with no morphological or histological differences detected between E2 treated ovaries and those from a control group. The control group was composed mostly of males (82.61%). Overall, there was no effect of E2 on survival ( P = 0.310) and growth rate data suggested no statistical differences ( P = 0.0805). However, the growth rate of the high-dose group increased slightly higher after the treatment ended than the other treatments ( P = 0.042), suggesting that E2 might suppress growth in green sunfish. In addition, the control group did not exhibit a higher survival rate after the treatment period ended ( P = 0.266), whereas both E2 treated groups did ( P = 0.0003–0.0050). We found that the low dose, 100 E2 mg per kg of diet, was sufficient for fully feminizing green sunfish if administered during development from 30 to 90 days post-hatch and E2 dosages may result in deleterious effects on green sunfish's health and growth.

Aquaculture↗

Incubation stage and polychlorinated biphenyl (PCB) congener patterns in an altricial and precocial bird species

The composition of polychlorinated biphenyl (PCB) congeners was compared between non-incubated and embryonated eggs of tree swallows ( Tachycineta bicolor ) and little terns ( Sterna albifrons ) to determine if measurable changes in PCB congeners occurred during the embryonic period. There was no indication of changes in PCB congener patterns over the incubation period in tree swallows in 1999 and 2000 at a site with very high PCB exposure or a site with more modest PCB exposure. Additionally, congeners known to be either quickly metabolized or conserved based on experimental studies did not generally respond as predicted. Similarly, PCB congener patterns in eggs of little terns from Bottsand, Schleswig-Holstein, Germany, did not differ between non-incubated and embryonated eggs. The results from both species suggest that the stage of incubation is not an important consideration when evaluating PCB congener patterns; comparisons and assessments can be made with eggs collected at all stages of incubation.

Environmental Pollution↗

Gut microbiome composition associates with corticosteroid treatment, morbidity, and senescence in Chinook salmon (Oncorhynchus tshawytscha)

Pacific salmon experience prolonged elevation in corticosteroid hormones during important life history events including migration, reproduction, and senescence. These periods of elevated corticosteroids correspond with changes to immunity and energy metabolism; therefore, fish may be particularly vulnerable to mortality at these times. Recent studies found that stress-induced cortisol release associated with microbial community shifts in salmonids, raising the question of how longer-term corticosteroid dynamics that accompany life history transitions affect salmonid microbiomes. In this work, we experimentally evaluated the relationships between gut microbiome composition, chronically elevated corticosteroids, and mortality in juvenile Chinook salmon ( Oncorhynchus tshawytscha) . We found that treatment with slow-release implants of the corticosteroids cortisol or dexamethasone resulted in persistent changes to the gut microbiome. Morbidity was also associated with microbiome composition, suggesting that the gut microbiome reflects individual differences in susceptibility to opportunistic pathogens. Additionally, we analyzed a small number of samples from adult fish at various stages of senescence. Results from these samples suggest that microbiome composition associated with gut integrity, and that the microbial communities of corticosteroid treated juveniles shift in composition toward those of senescent adults. Overall, findings from this work suggest that the gut microbiome associates with mortality risk during periods of chronic corticosteroid elevation.

Scientific Reports↗

Experimental challenge and pathology of highly pathogenic avian influenza virus H5N1 in dunlin ( Calidris alpina ), an intercontinental migrant shorebird species

Background Shorebirds (Charadriiformes) are considered one of the primary reservoirs of avian influenza. Because these species are highly migratory, there is concern that infected shorebirds may be a mechanism by which highly pathogenic avian influenza virus (HPAIV) H5N1 could be introduced into North America from Asia. Large numbers of dunlin ( Calidris alpina ) migrate from wintering areas in central and eastern Asia, where HPAIV H5N1 is endemic, across the Bering Sea to breeding areas in Alaska. Low pathogenic avian influenza virus has been previously detected in dunlin, and thus, dunlin represent a potential risk to transport HPAIV to North America. To date no experimental challenge studies have been performed in shorebirds. Methods Wild dunlin were inoculated intranasally and intrachoanally various doses of HPAIV H5N1. The birds were monitored daily for virus excretion, disease signs, morbidity, and mortality. Results The infectious dose of HPAIV H5N1 in dunlin was determined to be 10 1.7 EID 50 /100 μl and that the lethal dose was 10 1.83 EID 50 /100 μl. Clinical signs were consistent with neurotropic disease, and histochemical analyses revealed that infection was systemic with viral antigen and RNA most consistently found in brain tissues. Infected birds excreted relatively large amounts of virus orally (10 4 EID 50 ) and smaller amounts cloacally. Conclusions Dunlin are highly susceptible to infection with HPAIV H5N1. They become infected after exposure to relatively small doses of the virus and if they become infected, they are most likely to suffer mortality within 3–5 days. These results have important implications regarding the risks of transport and transmission of HPAIV H5N1 to North America by this species and raises questions for further investigation.

Alaska↗

Effects of exercise and bioprocessed soybean meal diets during rainbow trout rearing

Background: Alternative protein sources to fishmeal in fish feeds are needed. Objectives: Evaluate rearing performance of adult rainbow trout (Oncorhynchus mykiss) (initial weight 139.0 ±1.5 g, length 232.9 ± 0.8 mm, mean ± SE) fed one of the two isonitrogenous and isocaloric diets (46% protein, 16% lipid) and reared at one of the two levels of exercise (water velocities of either 3.6 cm/s or 33.2 cm/s). Methods: Protein in the control diet was based on fishmeal. In the experimental diet, bioprocessed soybean meal replaced approximately 60% of the fishmeal. Fish were fed by hand once-per-day to near satiation, and the food was increased daily. The experiment lasted 90-days. Results: There were no significant differences in gain, percent gain, or specific growth rate between the dietary treatments. However, the amount of food fed and feed conversion ratio was significantly lower in the 60% bioprocessed soybean meal diet. Intestinal morphology, relative fin length, splenosomatic index, hepatosomatic index, and viscerosomatic index were not significantly different in the trout fed either diet. Fish reared at 3.6 cm/s had a significantly lower feed conversion ratio (1.02 ± 0.02) than fish reared at 33.2 cm/s (1.13 ± 0.02). However, there were no significant differences in gain, percent gain, specific growth rate, or percentage mortality in fish reared with or without exercise. No significant interactions were observed between diet and exercise (higher water velocity). Conclusion:Based on these results, at least 60% of the fishmeal in adult rainbow trout diets can be replaced by bioprocessed soybean meal, even if higher water velocities are used to exercise the fish.

The Open Biology Journal↗