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The influence of gender on the relationship between wildlife value orientations, beliefs, and the acceptability of lethal deer control in Cuyahoga Valley National Park

This study examines how wildlife value orientations, attitudes, and gender influence acceptance of lethal actions to control deer in Cuyahoga Valley National Park in Ohio. Data were collected from female and male residents (n = 659) in a nine-county area, the primary service area of the park. Females and males demonstrated significant differences in their wildlife value orientations, attitudes toward lethal deer control, beliefs about the outcome of lethal deer control, and perceived personal impacts of lethal deer control. Gender also acted as a moderator of the relationship between values, beliefs and attitudes. Results indicate that a focus on understanding differences between males and females is essential to public participation in decision making concerning this and similar issues.

Society and Natural Resources

Statistical learning mitigation of false positives from template-detected data in automated acoustic wildlife monitoring

Audio sampling of the environment can provide long-term, landscape-scale presence-absence data to model populations of sound-producing wildlife. Automated detection systems allow researchers to avoid manually searching through large volumes of recordings, but often produce unacceptable false positive rates. We developed methods that allow researchers to improve template-based automated detection using a suite of statistical learning algorithms when false positive rates are problematic. To test our method, we acquired 668 hours of recordings in the Sonoran Desert, California USA between March 2016 and May 2017, and created spectrogram cross-correlation templates for three target avian species. We trained and tested five classification algorithms and four performance-weighted ensemble classifier methods on target signals and false alarms from March 2016, and then selected high-performing ensemble classifiers from the train/test phase to predict the class of new detections thereafter. For three target species, our ensemble classifiers were able to identify 98%, 81%, and 100% of false alarms compared with the baseline template detection system, and comparative positive predictive values improved from 6% to 69%, 87% to 95%, and 2% to 77%. We show that statistical learning approaches can be implemented to mitigate false detections acquired via template-based automated detection in automated acoustic wildlife monitoring.

California

Toxicity of Zectran insecticide to several wildlife species

Zectran® (4-dimethylamino-3,5-xylyl methycarbamate), all insecticide proposed for control of spruce budworm, Choristoneura fumiferana (Clemens), was tested against 16 species of pen-reared or captivity-acclimated animals to determine its toxicity and potential hazard to wildlife. The acute oral LD 60 values in mg/kg were: lesser sandhill crane, Grus canadensis canadensis L.., 1.0-4.5; Canada goose, Branta canadensis L.., 2.64; mourning dove, Zenaidura macroura L.., 2.83; mallard duck, Anas platyrhyndura L.., 3.0; coturnix quail, Coturnix coturnix japonica (Teminck & Schlep get), 3.21; mallard duckling 4.2; ring-necked pheasant, Phasianus colchicus L.., 4.5; house finch, Garpodacus mexicanus (Müller), 4.76; chukar partridge, Alectoris graeca (Meisner), 5.24; domestic pigeon, Columba livia (Gmelin), 6.47; sharp-tailed grouse, Pedioecetes phasianellus L.., 10.0; albino Sprague-Dawley rat, Rattus noruegicis (Fischer), 14.1; domestic goat, Capra aegagnzs L., 15.0-30.0; mule deer, Odocoileus hemionus (Rafinesque), 20.0-30.0; house (English) sparrow, Passer domesticus 1.., 50.4; and bullfrog, Rana catesbeiana (Shaw), 283.0-800.0. In general, birds were more susceptible to single oral doses than mammals and reacted more quickly to intoxication and were more quickly affected. In both groups, the first easily recognized symptom of Zectran poisoning was an increase in the respiratory rate. Deaths appeared to occur from respiratory failure following tachypnea and Cheyne-Stokes respiration. Zectran did not show any marked cumulative effect in subacute oral tests with mallards, chukars, and mule deer. The animals were able to tolerate up to about 40% of a single oral LD 50 dose per day for 30 days, and there was some evidence of a low degree of acquired resistance. Zectran applied dermally to albino New Zealand rabbits, Oryctolagus cuniculus (Lilljeborg), at 2000 mg/kg produced no discernible effects. The reproduction of chukars surviving repeated daily doses of Zectran was similar to that of controls. Low application rates, rapid degradation, low dermal toxicity, and the low degree of cumulative toxic action indicate that little hazard to wildlife should result under field conditions, even though Zectran is highly toxic in acute oral tests.

Journal of Economic Entomology

Subspecific affinity of black bears in the White River National Wildlife Refuge

The black bear population of the White River National Wildlife Refuge (NWR) is adjacent to populations of black bear in Louisiana ( Urusus americanus luteolus ) which are listed as threatened under the U.S. Endangered Species Act. Wildlife management plans can pose restrictions on bear harvests and timber extraction; therefore the management plan for the White River NWR is sensitive to subspecific classification of its bear population. The objective of this study was to analyze genetic variation in the White River NWR and seven adjacent populations of black bears to assess the subspecific affinity of the White River NWR population. Here we report the variation at seven microsatellite DNA loci among eight black bear populations. The patterns of genetic variation gave strong support for distinguishing a southern group of black bears comprised of the White River, Arkansas; Tensas River, Louisiana; Upper Atchafalaya, Louisiana; Lower Atchafalaya, Louisiana; and Alabama/Mississippi populations. Phylogenetic analysis of individual variation suggested that historical black bear introductions into Arkansas and Louisiana affected gene pools of certain southern receiving populations, but did not significantly change interpopulation relatedness. Phylogenetic inferences at both the population and individual levels support the hypothesis that the White River NWR population of black bears belongs to the U. a. luteolus subspecies.

Arkansas

Ecological feedbacks can reduce population-level efficacy of wildlife fertility control

1. Anthropogenic stress on natural systems, particularly the fragmentation of landscapes and the extirpation of predators from food webs, has intensified the need to regulate abundance of wildlife populations with management. Controlling population growth using fertility control has been considered for almost four decades, but nearly all research has focused on understanding effects of fertility control agents on individual animals. Questions about the efficacy of fertility control as a way to control populations remain largely unanswered. 2. Collateral consequences of contraception can produce unexpected changes in birth rates, survival, immigration and emigration that may reduce the effectiveness of regulating animal abundance. The magnitude and frequency of such effects vary with species-specific social and reproductive systems, as well as connectivity of populations. Developing models that incorporate static demographic parameters from populations not controlled by contraception may bias predictions of fertility control efficacy. 3. Many population-level studies demonstrate that changes in survival and immigration induced by fertility control can compensate for the reduction in births caused by contraception. The most successful cases of regulating populations using fertility control come from applications of contraceptives to small, closed populations of gregarious and easily accessed species. 4. Fertility control can result in artificial selection pressures on the population and may lead to long-term unintentional genetic consequences. The magnitude of such selection is dependent on individual heritability and behavioural traits, as well as environmental variation. 5. Synthesis and applications . Understanding species' life-history strategies, biology, behavioural ecology and ecological context is critical to developing realistic expectations of regulating populations using fertility control. Before time, effort and funding are invested in wildlife contraception, managers may need to consider the possibility that many species and populations can compensate for reduction in fecundity, and this could minimize any reduction in population growth rate.

Journal of Applied Ecology

Harvesting wildlife affected by climate change: a modelling and management approach for polar bears

The conservation of many wildlife species requires understanding the demographic effects of climate change, including interactions between climate change and harvest, which can provide cultural, nutritional or economic value to humans. We present a demographic model that is based on the polar bear Ursus maritimus life cycle and includes density-dependent relationships linking vital rates to environmental carrying capacity ( K ). Using this model, we develop a state-dependent management framework to calculate a harvest level that (i) maintains a population above its maximum net productivity level (MNPL; the population size that produces the greatest net increment in abundance) relative to a changing K , and (ii) has a limited negative effect on population persistence. Our density-dependent relationships suggest that MNPL for polar bears occurs at approximately 0·69 (95% CI = 0·63–0·74) of K . Population growth rate at MNPL was approximately 0·82 (95% CI = 0·79–0·84) of the maximum intrinsic growth rate, suggesting relatively strong compensation for human-caused mortality. Our findings indicate that it is possible to minimize the demographic risks of harvest under climate change, including the risk that harvest will accelerate population declines driven by loss of the polar bear's sea-ice habitat. This requires that (i) the harvest rate – which could be 0 in some situations – accounts for a population's intrinsic growth rate, (ii) the harvest rate accounts for the quality of population data (e.g. lower harvest when uncertainty is large), and (iii) the harvest level is obtained by multiplying the harvest rate by an updated estimate of population size. Environmental variability, the sex and age of removed animals and risk tolerance can also affect the harvest rate. Synthesis and applications . We present a coupled modelling and management approach for wildlife that accounts for climate change and can be used to balance trade-offs among multiple conservation goals. In our example application to polar bears experiencing sea-ice loss, the goals are to maintain population viability while providing continued opportunities for subsistence harvest. Our approach may be relevant to other species for which near-term management is focused on human factors that directly influence population dynamics within the broader context of climate-induced habitat degradation.

Journal of Applied Ecology

Improving estimation of flight altitude in wildlife telemetry studies

Altitude measurements from wildlife tracking devices, combined with elevation data, are commonly used to estimate the flight altitude of volant animals. However, these data often include measurement error. Understanding this error may improve estimation of flight altitude and benefit applied ecology. There are a number of different approaches that have been used to address this measurement error. These include filtering based on GPS data, filtering based on behaviour of the study species, and use of state-space models to correct measurement error. The effectiveness of these approaches is highly variable. Recent studies have based inference of flight altitude on misunderstandings about avian natural history and technical or analytical tools. In this Commentary, we discuss these misunderstandings and suggest alternative strategies both to resolve some of these issues and to improve estimation of flight altitude. These strategies also can be applied to other measures derived from telemetry data. Synthesis and applications. Our Commentary is intended to clarify and improve upon some of the assumptions made when estimating flight altitude and, more broadly, when using GPS telemetry data. We also suggest best practices for identifying flight behaviour, addressing GPS error, and using flight altitudes to estimate collision risk with anthropogenic structures. Addressing the issues we describe would help improve estimates of flight altitude and advance understanding of the treatment of error in wildlife telemetry studies.

Journal of Applied Ecology

Spatially explicit power analyses to inform occupancy‐based multi‐species wildlife monitoring programmes

1. Current and accurate information on wildlife populations is integral to successful biodiversity management and conservation globally. Nevertheless, many monitoring programs fail in their attempts to accurately monitor populations of interest due to interlinked issues including insufficient sample sizes, inappropriate duration, lack of reproducibility, and lack of clearly stated objectives. These common pitfalls could be avoided through the elicitation of explicit monitoring objectives and the a priori use of simulations to inform minimum sampling design requirements to meet said objectives. 2. Here, we provide a blueprint for using spatially explicit power analyses to inform the design and implementation of multi-species monitoring programs on landscape-scales. As a demonstration, we used spatially explicit simulations to devise a suitable sampling regime to meet clearly specified monitoring objectives in New York State: to use annual occupancy-based monitoring to be able to detect 25% and 50% changes in abundance of populations over five- and ten- year periods for all species of management interest in New York State, USA. We focused our simulation efforts on three challenging focal species (black bear, Ursus americanus, bobcat, Lynx rufus, and American marten, Martes americana) that differ notably in their morphology, life histories, space use, detection probability, habitat suitability, and population sizes/trajectories, and thus provide extremes in the challenges presented when it comes to sampling appropriately to detect changes in abundance. 3. Our simulations demonstrate variable context dependent trade-offs in sampling designs (i.e. number of sites [J] and number of sampling occasions [K]), and identify necessary minimum detection probabilities that must be attained to achieve statistical power to detect changes of varying magnitudes in populations of varying sizes in the three focal species. The simulations also highlight that monitoring population increases is likely beyond the reach of occupancy-based monitoring programs for wide-ranging or locally abundant species. 4. Synthesis and applications : We combine the results from the single-species simulations to produce a multi-species sampling design that meets the specified objectives for all three species. While the case study is centered on developing a multi-species sampling regime for New York State, it provides a reproducible step-by-step framework using established methods for wildlife managers and other practitioners to inform their own context- and objective- specific multi-species occupancy-based monitoring programs.

New York

Hydroclimate projections and effects on runoff at National Wildlife Refuges in the semi-arid western U.S.

This study evaluated hydroclimate projections and effects on runoff at National Wildlife Refuges in a semiarid region of the western United States (U.S. Fish and Wildlife Service Region 6) using mean air temperature (TAVE) and precipitation (PPT) inputs and runoff (RO) output from a national application of a Monthly Water Balance Model (MWBM). An ensemble of statistically downscaled global circulation models for two future emissions scenarios from Coupled Model Intercomparison Project 3 and 5 (CMIP3 and 5) were assessed at the refuges for the years 1950–2099. TAVE, PPT, and RO and departures from mean baseline conditions were analyzed from MWBM hydrologic response units within refuge boundaries. Seasonal results were evaluated across four periods: historical (1951–1969), baseline (1981–1999), 2050 (2041–2059), and 2080 (2071–2089). Projected TAVE increases for all refuges and time periods, whereas PPT and RO are much more variable across ecoregions. Using the high emission scenario, summer mean monthly TAVE increases range from 4.8°C to 5.5°C by 2080. Summer mean monthly PPT departures vary from −5.7 to 3.9 mm (up to 14% decrease), with decreases at 41% of refuges. Summer RO departures range from −16.7 to 0.2 mm (up to 60% decrease), with decreases at 71% of refuges. Under the same emission scenario, winter PPT and RO increase at most refuges by 2080. These variable departures will create substantial challenges for future conservation management in the region.

JAWRA Journal of the American Water Resources Asso

Quantitative support for the benefits of proactive management for wildlife disease control

Finding effective pathogen mitigation strategies is one of the biggest challenges humans face today. In the context of wildlife, emerging infectious diseases have repeatedly caused widespread host morbidity and population declines of numerous taxa. In areas yet unaffected by a pathogen, a proactive management approach has the potential to minimize or prevent host mortality. However, typically critical information on disease dynamics in a novel host system is lacking, empirical evidence on efficacy of management interventions is limited, and there is a lack of validated predictive models. As such, quantitative support for identifying effective management interventions is largely absent, and the opportunity for proactive management is often missed. We considered the potential invasion of the chytrid fungus, Batrachochytrium salamandrivorans (Bsal), whose expected emergence in North America poses a severe threat to hundreds of salamander species in this global salamander biodiversity hotspot. We developed and parameterized a dynamic multistate occupancy model to forecast host and pathogen occurrence, following expected emergence of the pathogen, and evaluated the response of salamander populations to different management scenarios. Our model forecasted that taking no action is expected to be catastrophic to salamander populations. Proactive action was predicted to maximize host occupancy outcomes relative to wait-and-see reactive management, thus providing quantitative support for proactive management opportunities. The eradication of Bsal was unlikely under all the evaluated management options. Contrary to our expectations, even early pathogen detection had little effect on Bsal or host occupancy outcomes. Our results provide quantitative support that proactive management is the optimal strategy for promoting persistence of disease-threatened salamander populations. Our approach fills a critical gap by defining a framework for evaluating management options prior to pathogen invasion and can thus serve as a template for addressing novel disease threats that jeopardize wildlife and human health.

Conservation Biology

Polar bears from space: Assessing satellite imagery as a tool to track Arctic wildlife

Development of efficient techniques for monitoring wildlife is a priority in the Arctic, where the impacts of climate change are acute and remoteness and logistical constraints hinder access. We evaluated high resolution satellite imagery as a tool to track the distribution and abundance of polar bears. We examined satellite images of a small island in Foxe Basin, Canada, occupied by a high density of bears during the summer ice-free season. Bears were distinguished from other light-colored spots by comparing images collected on different dates. A sample of ground-truthed points demonstrated that we accurately classified bears. Independent observers reviewed images and a population estimate was obtained using mark- recapture models. This estimate (N: 94; 95% Confidence Interval: 92-105) was remarkably similar to an abundance estimate derived from a line transect aerial survey conducted a few days earlier (N: 102; 95% CI: 69-152). Our findings suggest that satellite imagery is a promising tool for monitoring polar bears on land, with implications for use with other Arctic wildlife. Large scale applications may require development of automated detection processes to expedite review and analysis. Future research should assess the utility of multi-spectral imagery and examine sites with different environmental characteristics.

PLoS ONE

Evaluating the effectiveness of wildlife detection and observation technologies at a solar power tower facility

Solar power towers produce electrical energy from sunlight at an industrial scale. Little is known about the effects of this technology on flying animals and few methods exist for automatically detecting or observing wildlife at solar towers and other tall anthropogenic structures. Smoking objects are sometimes observed co-occurring with reflected, concentrated light (“solar flux”) in the airspace around solar towers, but the identity and origins of such objects can be difficult to determine. In this observational pilot study at the world’s largest solar tower facility, we assessed the efficacy of using radar, surveillance video, and insect trapping to detect and observe animals flying near the towers. During site visits in May and September 2014, we monitored the airspace surrounding towers and observed insects, birds, and bats under a variety of environmental and operational conditions. We detected and broadly differentiated animals or objects moving through the airspace generally using radar and near solar towers using several video imaging methods. Video revealed what appeared to be mostly small insects burning in the solar flux. Also, we occasionally detected birds flying in the solar flux but could not accurately identify birds to species or the types of insects and small objects composing the vast majority of smoking targets. Insect trapping on the ground was somewhat effective at sampling smaller insects around the tower, and presence and abundance of insects in the traps generally trended with radar and video observations. Traps did not tend to sample the larger insects we sometimes observed flying in the solar flux or found dead on the ground beneath the towers. Some of the methods we tested (e.g., video surveillance) could be further assessed and potentially used to automatically detect and observe flying animals in the vicinity of solar towers to advance understanding about their effects on wildlife.

California

Differential changes in the onset of spring across US National Wildlife Refuges and North American migratory bird flyways

Warming temperatures associated with climate change can have indirect effects on migratory birds that rely on seasonally available food resources and habitats that vary across spatial and temporal scales. We used two heat-based indices of spring onset, the First Leaf Index (FLI) and the First Bloom Index (FBI), as proxies of habitat change for the period 1901 to 2012 at three spatial scales: the US National Wildlife Refuge System; the four major bird migratory flyways in North America; and the seasonal ranges (i.e., breeding and non-breeding grounds) of two migratory bird species, Blue-winged Warbler ( Vermivora cyanoptera ) and Whooping Crane ( Grus americana ). Our results show that relative to the historical range of variability, the onset of spring is now earlier in 76% of all wildlife refuges and extremely early (i.e., exceeding 95% of historical conditions) in 49% of refuges. In all flyways but the Pacific, the rate of spring advance is generally greater at higher latitudes than at lower latitudes. This differential rate of advance in spring onset is most pronounced in the Atlantic flyway, presumably because of a “warming hole” in the southeastern US. Both FLI and FBI have advanced markedly in the breeding ranges–but not the non-breeding ranges–of the two selected bird species, albeit with considerable intra-range variation. Differences among species in terms of migratory patterns and the location and extent of seasonal habitats, as well as shifts in habitat conditions over time, may complicate predictions of the vulnerability of migratory birds to climate change effects. This study provides insight into how differential shifts in the phenology of disparate but linked habitats could inform local- to landscape-scale management strategies for the conservation of migratory bird populations.

PLoS ONE

Wildlife resistance and protection in a changing New England landscape

Rapid changes in climate and land use threaten the persistence of wildlife species. Understanding where species are likely to occur now and in the future can help identify areas that are resistant to change over time and guide conservation planning. We estimated changes in species distribution patterns and spatial resistance in five future scenarios for the New England region of the northeastern United States. We present scenario-specific distribution change maps for nine harvested wildlife species, identifying regions of increasing, decreasing, or stable habitat suitability within each scenario. Next, we isolated areas of greatest resistance across all future scenarios. Resistance was also evaluated relative to current land protection to identify patterns in and out of Protected Areas (PAs). Generally, species distributions declined in area over the 50-year assessment period (2010-2060), with the greatest declines occurring for moose (62.4%) and wild turkey (24.2%). Species resistance varied considerably across the region with coyote demonstrating the highest regional resistance (91.81% of the region) and moose demonstrating the lowest (0.76 % of the region). At the state level, average focal species resistance was highest in Maine and lowest in Massachusetts. Many of the focal species showed high overlap in resistance and land protection. Coyote, white-tailed deer, and black bear had the highest representation of resistance within PAs, while moose and wild turkey had the largest proportions of regional resistance occurring within PAs. Overall, relatively small portions of New England – ranging between 0.25% and 21.12% – were both protected and resistant for the focal species. Our results provide estimates of resistance that can inform conservation planning for commonly harvested species that are important ecologically, economically, and culturally to the region. Expanding protected area coverage to include resistant areas may provide longer term benefits to these species.

Connecticut, Maine, Massachusetts, New Hampshire,

A state-based national network for effective wildlife conservation

State wildlife conservation programs provide a strong foundation for biodiversity conservation in the United States, building on state wildlife action plans. However, states may miss the species that are at the most risk at rangewide scales, and threats such as novel diseases and climate change increasingly act at regional and national levels. Regional collaborations among states and their partners have had impressive successes, and several federal programs now incorporate state priorities. However, regional collaborations are uneven across the country, and no national counterpart exists to support efforts at that scale. A national conservation-support program could fill this gap and could work across the conservation community to identify large-scale conservation needs and support efforts to meet them. By providing important information-sharing and capacity-building services, such a program would advance collaborative conservation among the states and their partners, thus increasing both the effectiveness and the efficiency of conservation in the United States.

BioScience

Ecotoxicology of mercury in fish and wildlife: Recent advances

A number of recent studies have documented subtle, yet potentially important effects of mercury on behavior, neurochemistry, and endocrine function in fish and wildlife at currently realistic levels of environmental exposure. Current levels of environmental methylmercury exposure are sufficient to cause significant biological impairment, both in individuals and in whole populations, in some ecosystems. Future toxicological studies on fish and wildlife will focus on linking biomarkers of methylmercury exposure and associated oxidative stress to effects on reproduction and population change; determining the genetic basis for mercury-related neurotoxic and other biological changes; determining the genetic basis for species differences in sensitivity to methylmercury; and linking toxic effects of methylmercury in individual animals to population-level changes.

Book chapter

Spatial and temporal patterns of chronic wasting disease: Fine-scale mapping of a wildlife epidemic in Wisconsin

Emerging infectious diseases threaten wildlife populations and human health. Understanding the spatial distributions of these new diseases is important for disease management and policy makers; however, the data are complicated by heterogeneities across host classes, sampling variance, sampling biases, and the space-time epidemic process. Ignoring these issues can lead to false conclusions or obscure important patterns in the data, such as spatial variation in disease prevalence. Here, we applied hierarchical Bayesian disease mapping methods to account for risk factors and to estimate spatial and temporal patterns of infection by chronic wasting disease (CWD) in white-tailed deer (Odocoileus virginianus) of Wisconsin, USA. We found significant heterogeneities for infection due to age, sex, and spatial location. Infection probability increased with age for all young deer, increased with age faster for young males, and then declined for some older animals, as expected from disease-associated mortality and age-related changes in infection risk. We found that disease prevalence was clustered in a central location, as expected under a simple spatial epidemic process where disease prevalence should increase with time and expand spatially. However, we could not detect any consistent temporal or spatiotemporal trends in CWD prevalence. Estimates of the temporal trend indicated that prevalence may have decreased or increased with nearly equal posterior probability, and the model without temporal or spatiotemporal effects was nearly equivalent to models with these effects based on deviance information criteria. For maximum interpretability of the role of location as a disease risk factor, we used the technique of direct standardization for prevalence mapping, which we develop and describe. These mapping results allow disease management actions to be employed with reference to the estimated spatial distribution of the disease and to those host classes most at risk. Future wildlife epidemiology studies should employ hierarchical Bayesian methods to smooth estimated quantities across space and time, account for heterogeneities, and then report disease rates based on an appropriate standardization. ?? 2009 by the Ecological Society of America.

Wisconsin

Wildlife effects of DDT dust used for tick control on a Texas prairie

The effect of DDT dust on wildlife was studied at Camp Bullis, Bexar County, Texas, in the summer of 1947. Studies were made on a 206.6 acre plot that was treated with DDT for experimental control of the Lone Star tick ( Amblyomma americanum ). A dust consisting of one part of DDT to nine parts of pyrophyllite was applied at an average rate of 4.4 pounds of DDT per acre. The limits of DDT concentration that affected wildlife cannot be stated exactly because of a heavy rain that fell near the end of the dusting, and because of irregularity in DDT deposition. Since absolute uniformity of dusting could not be expected in any large scale DDT application, the effects observed in these trials were probably fairly representative. However, continued dry weather would have permitted longer exposure to DDT, possibly with more severe effects than those found in this study. The vegetation of the experimental area was roughly 70 percent ungrazed tall-grass prairie and 30 percent trees and shrubs. Ground and bush feeding birds were severely affected. Cardinals, lark sparrows, field sparrows, Bewick's wrens, Carolina wrens, Kentucky warblers, yellow-breasted chats, blue grosbeaks, and painted buntings were nearly or entirely eliminated from the treated area. Birds affected, but less drastically reduced in numbers, were yellow-billed cuckoo, black and white warbler, yellow-throated vireo, and white-eyed vireo. Birds found dead in the DDT area were 9 cardinals, 2 painted buntings, 2 lark sparrows, 1 yellow-breasted chat, and 1 white-eyed vireo. Bird mortality had begun by the day after dusting and was largely over by the end of the fifth day. Census of deer in DDT and check areas before and after treatment showed no reduction in deer numbers and no diminution in use of the DDT area. No deer or fawns were found dead or affected. Box-trapping of raccoons in DDT and check areas before and after treatment showed no effects that could be attributed to DDT. Limited observations on armadillos, striped skunks, and rabbits gave no indication of pronounced damage to these forms. No mammals of any kind were found dead or affected in or near the DDT area. Four rough green snakes and one Texan spiny lizard were found dead in the DDT area. Mortality was probably high among insectivorous reptiles.

Texas