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At least 559 records · Page 31Linked to original sources

Early evolution of Tubulogenerina during the Paleogene of Europe

The early evolution of Tubulogenerina took place in Europe where eight species occur in lower Eocene to uppermost Oligocene or lower Miocene strata. Species diversity within Tubulogenerina dropped significantly in the early Oligocne; only a single species persisted from the late Eocene, and it became extinct before the end of the early Oligocene. Morphologic changes during the European phylogeny of Tubulogenerina include (1) the development of costate and more complex tubulopore ornamentation, and (2) the change from a single elongated apertural slit with a single toothplate to multiple apertures and toothplates. Three new Tubulogenerina species are described. -from Authors

Journal of Foraminiferal Research↗

An eddy-resolving numerical model to study turbulent flow, sediment and bed evolution using detached eddy simulation in a lateral separation zone at the field-scale

Turbulence-resolving simulations elucidate key elements of fluid dynamics and sediment transport in fluvial environments. This research presents a feasible strategy for applying state-of-the-art computational fluid mechanics to the study of sediment transport and morphodynamic processes in lateral separation zones, which are common features in canyon rivers where massive lateral flow separation causes large-scale turbulence that controls sediment erosion and deposition. An eddy-resolving model was developed and tested at the field-scale, coupling a viscous flow and sediment transport solver using Detached Eddy Simulation techniques. A morphodynamic model was applied to the viscous flow/sediment solver to calculate erosion and deposition. A simulation of turbulence was performed at the grid resolution for a straight channel to determine the relative contributions of modeled and resolved diffusivity. The time-dependent, energetically important, correlative, non-stationary signals of the simulated quantities were captured at the lateral separation zone. Strong periodic signals featured by high amplitude were found at the separation zone, while low frequency pulsations were observed at the reattachment zone of the lateral separation zone. Interactions between the eddies and the loose bed boundaries resulted in erosion of sediment at the main channel followed by deposition at the primary eddy and eddy bars. tions elucidate key elements of fluid dynamics and sediment transport in fluvial environments. This research presents a feasible strategy for applying state-of-the-art computational fluid mechanics to the study of sediment transport and morphodynamic processes in lateral separation zones, which are common features in canyon rivers where massive lateral flow separation causes large-scale turbulence that controls sediment erosion and deposition. An eddy-resolving model was developed and tested at the field scale, coupling viscous flow and sediment transport solver using Detached Eddy Simulation (DES) techniques. A morphodynamic model was applied to the viscous flow/sediment solver to calculate erosion and deposition. A simulation of turbulence was performed at the grid resolution for a straight channel to determine the relative contributions of modeled and resolved diffusivity. The time-dependent, energetically important, correlative non-stationary signals of the simulated quantities were captured at the lateral separation zone. Strong periodic signals featured by high amplitude were found at the separation zone, while low frequency pulsations were observed at the reattachment zone of the lateral separation zone. Interactions between the eddies and the loose bed boundaries resulted in massive erosion of sediment at the main channel followed by deposition at the primary eddy and eddy bars.

Arizona↗

Petrogenetic evolution of the torfajökull volcanic complex, Iceland I. relationship between the magma types

The Torfajökull volcano, Iceland's largest silicic centre, is situated close to the junction of the active, southerly propagating Eastern Rift Zone and the South Eastern Zone, an older crustal segment. This paper provides major, trace, and some Sr isotope data on postglacial (<10000 y) rocks, i.e., tholeiitic magmas of the Eastern Rift Zone and transitional basalts, icelandites, and rhyolites of the Torfajökull centre, and assesses the relationships between the magma types in terms of the development of the Icelandic crust.Tholeiitic magmas from the Eastern Rift Zone are LILE-enriched relative to MORB. They have undergone extensive olivine-plagioclase-clinopyroxene fractionation at low pressures. Compared with the tholeiites, Torfajökull transitional basalts show LILE/HFS enrichment and higher ( 87 Sr/ 86 Sr) 1 ratios. They define several magmatic lineages and have equilibrated over a wide range of pressures. Both basalt types were derived by very small degrees of partial melting of compositionally similar mantle sources, the main difference being that the tholeiites were generated in the spinel Iherzolite, and the transitional basalts in the garnet lherzolite, stability fields, a conclusion previously reached by Meyer et al. (1985). The mantle sources may have contained LILE-enriched streaks.Low-pressure differentiation of Torfajökull transitional basalt produced an iceiandite to sub-alkaline rhyolite sequence by crystal fractionation, the rhyolites representing >90% crystallization of parental basalts. The rhyolites were emplaced as nine separate lava fields, formed during 11 eruptive episodes. The compositional range within each field is limited, and, although similar, the ranges define several magmatic lineages. Continued fractionation of plagioclase-alkali feldspar-clinopyroxene-magnetite-apatite-zircon assemblages generated peralkaline rhyolites in certain post-glacial eruptions. Chemical variations in the deposits from the Hrafntinnusker peralkaline eruption were related predominantly to alkali feldspar fractionation, and the melts were erupted from a zoned magma chamber. All postglacial volcanic rocks at Torfajokull have been mantle derived and thus represent new additions to the Icelandic crust. © 1990 Oxford University Press.

Journal of Petrology↗

CO2 hydrate: Synthesis, composition, structure, dissociation behavior, and a comparison to structure I CH4 hydrate

Structure I (sI) carbon dioxide (CO 2 ) hydrate exhibits markedly different dissociation behavior from sI methane (CH 4 ) hydrate in experiments in which equilibrated samples at 0.1 MPa are heated isobarically at 13 K/h from 210 K through the H 2 O melting point (273.15 K). The CO 2 hydrate samples release only about 3% of their gas content up to temperatures of 240 K, which is 22 K above the hydrate phase boundary. Up to 20% is released by 270 K, and the remaining CO 2 is released at 271.0 plusmn; 0.5 K, where the sample temperature is buffered until hydrate dissociation ceases. This reproducible buffering temperature for the dissociation reaction CO 2 ·nH 2 O = CO 2 (g) + nH 2 O(1 to s) is measurably distinct from the pure H 2 O melting point at 273.15 K, which is reached as gas evolution ceases. In contrast, when si CH 4 hydrate is heated at the same rate at 0.1 MPa, >95% of the gas is released within 25 K of the equilibrium temperature (193 K at 0.1 MPa). In conjunction with the dissociation study, a method for efficient and reproducible synthesis of pure polycrystalline CO 2 hydrate with suitable characteristics for material properties testing was developed, and the material was characterized. CO 2 hydrate was synthesized from CO 2 liquid and H 2 O solid and liquid reactants at pressures between 5 and 25 MPa and temperatures between 250 and 281 K. Scanning electron microscopy (SEM) examination indicates that the samples consist of dense crystalline hydrate and 50-300 μm diameter pores that are lined with euhedral cubic hydrate crystals. Deuterated hydrate samples made by this same procedure were analyzed by neutron diffraction at temperatures between 4 and 215 K; results confirm that complete conversion of water to hydrate has occurred and that the measured unit cell parameter and thermal expansion are consistent with previously reported values. On the basis of measured weight gain after synthesis and gas yields from the dissociation experiments, approximately all cages in the hydrate structure are filled such that n ≈ 5.75.

Journal of Physical Chemistry B↗

Global seismic networks operated by the U.S. Geological Survey

The U.S. Geological Survey (USGS) Global Seismographic Network (GSN) Program operates two thirds of the GSN, a network of state‐of‐the‐art, digital seismological and geophysical sensors with digital telecommunications. This network serves as a multiuse scientific facility and a valuable resource for research, education, and monitoring. The other one third of the GSN is funded by the National Science Foundation (NSF), and the operations of this component are overseen by EarthScope. This collaboration between the USGS, EarthScope, and NSF has allowed for the development and operations of the GSN to be a truly multiuse network that provides near real‐time open access data, facilitating fundamental discoveries by the Earth science community, supporting the earthquake hazards mission of the USGS, benefitting tsunami monitoring by the National Oceanic and Atmospheric Administration, and contributing to nuclear test monitoring and treaty verification. In this article, we describe the installation and evolution of the seismic networks operated by the USGS that ultimately led to the USGS portion of the GSN (100 stations under network codes IU, IC, and CU) as they are today and envision technological advances and opportunities to further improve the utility of the network in the future. This article focuses on the USGS‐operated component of the GSN; a companion article on the GSN stations funded by the NSF and operated by the Cecil and Ida Green Institute of Geophysics and Planetary Physics, Scripps Institution of Oceanography, University of California at San Diego by Davis et al. (2023) appears in this volume.

Seismological Research Letters↗

A geologic history of the north-central Appalachians, part 2: The Appalachian basin from the Silurian through the Carboniferous

The north-central Appalachians occupy a critical position within the 3000+ km-long Appalachian orogen, lying southwest of the boundary between the central and northern Appalachians (CNAB). The one-billion-year-long history of tectonic activity in the north-central Appalachians includes the assembly and breakup of a late Proterozoic supercontinent, the creation and evolution of the Appalachian orogen during the Paleozoic, and the Mesozoic transformation of the active orogen into a passive margin during Pangea's disassembly. An important part of the Middle and Late Paleozoic history is the formation and growth of the Appalachian basin, an enormous, elongate continental basin lying cratonward of the active Appalachian internides along Laurentia's eastern margin. The Appalachian basin developed out of the Taconic orogeny, the second of the four orogenies that formed and modified the character of the Appalachian orogen. Prior to the Taconic orogeny, the eastern margin of Laurentia consisted of a broad carbonate shelf facing the Theic Ocean. The Taconic orogeny obduced various Theic components, including microcontinents, magmatic arcs, and accretionary prisms, onto the carbonate shelf. The resulting Taconic highlands formed a topographic barrier between Theia and the craton's interior. Because tectonic activity in the internides continued more or less throughout the remainder of the Paleozoic, the Appalachian basin never had a direct connection with Theia. The coarse-grained molasse from the newly uplifted Taconic highland spread westward over most of the basin during the Early Silurian. Carbonate deposition gradually encroached eastward over the basin as the siliciclastic input from the southeast waned. A resurgence in the earliest Late Silurian, perhaps related to orogenesis in Newfoundland and the Maritimes, expanded the clastic wedge somewhat. Before long, however, carbonate deposition once again dominated most of the north-central basin for the remainder of the Silurian and into the Early Devonian. The Early-to-Middle Devonian Acadian orogeny began introducing siliciclastic material into the eastern part of the Appalachian basin, thereby ending the largely paralic environment that persisted from the Late Silurian. A number of deltas formed around local sediment-input centers during the Middle Devonian. Continued uplift and/or orogenesis must have continued into the Late Devonian because a vast amount of terrigenous sediment was introduced into the Appalachian basin to form the vast Upper Devonian Catskill delta. Although the principal tectonic activity appears to have centered in New England, the large volume of sediment input suggests that orogenesis may have extended southward to the north-central Appalachians. However, direct evidence of actual Acadian deformation and metamorphism is lacking at this latitude. The north-central Appalachian basin underwent a significant change during the Carboniferous. Prior to this time, the shoreline shifted laterally through transgressions and regressions largely as a function of sediment input and regional subsidence. In the Carboniferous, vertical movements in the basin became more important. During the Early Carboniferous, much of the Mauch Chunk delta was eroded in the central and western parts of the basin. At the end of the Early Carboniferous, an unconformity truncated progressively older rocks to the north toward New York. New sediment transport patterns and depositional environments within the basin during the Late Carboniferous probably reflect significant topographic and tectonic changes, not only in the hinterland, but in the basin itself, perhaps in conjunction with climatic changes. Extensive interfluvial swamps on a lower delta plain accumulated thick organic deposits which would become coal. This delta plain graded southwestward into tidal and marine environments. The Alleghany orogeny in the Early Permian interrupted deposition in much of the north-central Appalachian basin and profoundly altered its structure.

American Journal of Science↗

Tectonic map of Indonesia: A progress report

Orogeny, volcanism, and seismicity are now intensely active in Indonesia. Many Dutch tectonists--Brouwer, Umbgrove, van Bemifielen, Smit4Sibinga, Vening Meinesz, Westerveld, and others--recognized that this complex cluster of islands represents an early stage in the evolution of orogenic belts. Not until Indonesia is understood can we comprehend the Alps. This report summarizes some aspects of work to date on the Tectonic Map of Indonesia. The preparation of this map is a joint project of the Geological Survey of Indonesia and the United States Geological Survey, sponsored by the Government of Indonesia and the United States Agency for International Development. The Tectonic Map of Indonesia will be published at a scale of 1:5,000,000. Adjacent regions in other countries will be included to provide a broader context. The map limits presently envisaged are the parallels of 12° N. and 15° S., and the meridians of 91° and 148° E. Tectonic features will be shown in many colors and patterns. Bathymetry is being newly compiled, and will be shown with contours and shades of blue. Figure 1 shows the islands of Indonesia.

Open-File Report↗

Evolution of the MOSS geographic information system for 32-bit computer systems

The authors discuss the current status and plans regarding the 32-bit implementation of the Map Overly and Statistical System (MOSS) geographic information system. Increasing interest in this system is promoting significant expansion of its capabilities, but any such enhancements will require careful analysis and planning to ensure that the resulting system is functionally complete and efficient yet flexible enough to adapt to a variety of user requirements and systems. All enhancements must be coordinated among centers of development in order to ensure the continued viability of a single MOSS. A baseline software configuration must be defined, and procedures must be developed to ensure coordination of any modifications to the baseline. Finally, there is significant interest in maintaining the public-domain aspects of MOSS to promote its shared use in the Department of the Interior. For these reasons, coordination efforts such as those initiated by the IDCCC and the MOSS Users Workshop should be strongly supported by the MOSS user community.

Conference Paper↗

Geomorphic history of Lake Manix, Mojave Desert, California: Evolution of a complex terminal lake basin

The US Environmental Protection Agency's short-term freshwater effluent test methods include a fish ( Pimephales promelas ), a cladoceran ( Ceriodaphnia dubia ), and a green alga ( Raphidocelis subcapitata ). There is a recognized need for additional taxa to accompany the three standard species for effluent testing. An appropriate additional taxon is unionid mussels because mussels are widely distributed, live burrowed in sediment and filter particles from the water column for food, and exhibit high sensitivity to a variety of contaminants. Multiple studies were conducted to develop a relevant and robust short-term test method for mussels. We first evaluated the comparative sensitivity of two mussel species ( Villosa constricta and Lampsilis siliquoidea ) and two standard species ( P. promelas and C. dubia ) using two mock effluents prepared by mixing ammonia and five metals (cadmium, copper, nickel, lead, and zinc) or a field-collected effluent in 7-day exposures. Both mussel species were equally or more sensitive (more than two-fold) to effluents compared with the standard species. Next, we refined the mussel test method by first determining the best feeding rate of a commercial algal mixture for three age groups (1, 2, and 3 weeks old) of L. siliquoidea in a 7-day feeding experiment, and then used the derived optimal feeding rates to assess the sensitivity of the three ages of juveniles in a 7-day reference toxicant (sodium chloride [NaCl]) test. Juvenile mussels grew substantially (30%–52% length increase) when the 1- or 2-week-old mussels were fed 2 ml twice daily and the 3-week-old mussels were fed 3 ml twice daily. The 25% inhibition concentrations (IC25s) for NaCl were similar (314–520 mg Cl/L) among the three age groups, indicating that an age range of 1- to 3-week-old mussels can be used for a 7-day test. Finally, using the refined test method, we conducted an interlaboratory study among 13 laboratories to evaluate the performance of a 7-day NaCl test with L. siliquoidea . Eleven laboratories successfully completed the test, with more than 80% control survival and reliable growth data. The IC25s ranged from 296 to 1076 mg Cl/L, with a low (34%) coefficient of variation, indicating that the proposed method for L. siliquoidea has acceptable precision. Environ Toxicol Chem 2021;40:3392–3409. © 2021 SETAC

California↗

Evolution of the Rodgers Creek–Maacama right-lateral fault system and associated basins east of the northward-migrating Mendocino Triple Junction, northern California

The Rodgers Creek–Maacama fault system in the northern California Coast Ranges (United States) takes up substantial right-lateral motion within the wide transform boundary between the Pacific and North American plates, over a slab window that has opened northward beneath the Coast Ranges. The fault system evolved in several right steps and splays preceded and accompanied by extension, volcanism, and strike-slip basin development. Fault and basin geometries have changed with time, in places with younger basins and faults overprinting older structures. Along-strike and successional changes in fault and basin geometry at the southern end of the fault system probably are adjustments to frequent fault zone reorganizations in response to Mendocino Triple Junction migration and northward transit of a major releasing bend in the northern San Andreas fault. The earliest Rodgers Creek fault zone displacement is interpreted to have occurred ca. 7 Ma along extensional basin-forming faults that splayed northwest from a west-northwest proto-Hayward fault zone, opening a transtensional basin west of Santa Rosa. After ca. 5 Ma, the early transtensional basin was compressed and extensional faults were reactivated as thrusts that uplifted the northeast side of the basin. After ca. 2.78 Ma, the Rodgers Creek fault zone again splayed from the earlier extensional and thrust faults to steeper dipping faults with more north-northwest orientations. In conjunction with the changes in orientation and slip mode, the Rodgers Creek fault zone dextral slip rate increased from ∼2–4 mm/yr 7–3 Ma, to 5–8 mm/yr after 3 Ma. The Maacama fault zone is shown from several data sets to have initiated ca. 3.2 Ma and has slipped right-laterally at ∼5–8 mm/yr since its initiation. The initial Maacama fault zone splayed northeastward from the south end of the Rodgers Creek fault zone, accompanied by the opening of several strike-slip basins, some of which were later uplifted and compressed during late-stage fault zone reorganization. The Santa Rosa pull-apart basin formed ca. 1 Ma, during the reorganization of the right stepover geometry of the Rodgers Creek–Maacama fault system, when the maturely evolved overlapping geometry of the northern Rodgers Creek and Maacama fault zones was overprinted by a less evolved, non-overlapping stepover geometry. The Rodgers Creek–Maacama fault system has contributed at least 44–53 km of right-lateral displacement to the East Bay fault system south of San Pablo Bay since 7 Ma, at a minimum rate of 6.1–7.8 mm/yr.

Geosphere↗

Volcano geodesy: A critical tool for assessing the state of volcanoes and their potential for hazardous eruptive activity

Since the beginning of the 20th century, volcano geodesy has evolved from time- and personnel-intensive methods for collecting discrete measurements to automated and/or remote tools that provide data with exceptional spatiotemporal resolution. By acknowledging and overcoming limitations related to data collection and interpretation, geodesy becomes a powerful tool for forecasting the onset and tracking the evolution of volcanic eruptions. In addition, geodetic data can be used for novel applications, such as mapping surface and topographic change due to the emplacement of volcanic deposits, detecting volcanic plumes, and constraining the properties of magmatic systems. These collective capabilities provide critical support for understanding magmatic processes at erupting volcanoes, while also offering important baseline data in advance of potential volcanic unrest. Future developments in volcano geodesy will involve not just new technology, but also advanced modeling and automated analysis methods that will provide a new understanding of the volcanic activity.

Book chapter↗

Online-coupling of widely-ranged timescales to model coral reef development

The increasing pressure on Earth's ecosystems due to climate change is becoming more and more evident and the impacts of climate change are especially visible on coral reefs. Understanding how climate change interacts with the physical environment of reefs to impact coral growth and reef development is critically important to predicting the persistence of reefs into the future. In this study, a biophysical model was developed including four environmental factors in a feedback loop with the coral's biology: (1) light; (2) hydrodynamics; (3) temperature; and (4) pH. The submodels are online coupled, i.e. regularly exchanging information and feedbacks while the model runs. This ensures computational efficiency despite the widely-ranged timescales. The composed biophysical model provides a significant step forward in understanding the processes that modulate the evolution of coral reefs, as it is the first construction of a model in which the hydrodynamics are included in the feedback loop.

Environmental Modeling and Software↗

Stratigraphic, sedimentologic, and dendrogeomorphic analyses of rapid floodplain formation along the Rio Grande in Big Bend National Park, Texas

The channel of the lower Rio Grande in the Big Bend region rapidly narrows during years of low mean and peak flow. We conducted stratigraphic, sedimentologic, and dendrogeomorphic analyses within two long floodplain trenches to precisely reconstruct the timing and processes of recent floodplain formation. We show that the channel of the Rio Grande narrowed through the oblique and vertical accretion of inset floodplains following channel-widening floods in 1978 and 1990–1991. Vertical accretion occurred at high rates, ranging from 16 to 35 cm/yr. Dendrogeomorphic analyses show that the onset of channel narrowing occurred during low-flow years when channel bars obliquely and vertically accreted fine sediment. This initial stage of accretion occurred by both bed-load and suspended-load deposition within the active channel. Vegetation became established on top of these fine-grained deposits during years of low peak flow and stabilized these developing surfaces. Subsequent deposition by moderate floods (between 1.5 and 7 yr recurrence intervals) caused additional accretion at rapid rates. Suspended-sediment deposition was dominant in the upper deposits, resulting in the formation of natural levees at the channel margins and the deposition of horizontally bedded, fining-upward deposits in the floodplain trough. Overall, channel narrowing and floodplain formation occurred through an evolution from active-channel to floodplain depositional processes. High-resolution dendrogeomorphic analyses provide the ability to specifically correlate the flow record to the onset of narrowing, the establishment of riparian vegetation, the formation of natural levees, and ultimately, the conversion of portions of the active channel to floodplains.

Texas↗

North American Bird Banding and quantitative population ecology

Early bird-banding programs in North America were developed to provide descriptions of bird migration and movement patterns. This initial interest in description quickly evolved into more quantitative interests in two ways. There was (1) interest in quantifying migration and movement patterns, and (2) rapid recognition that re-observations of marked birds provided information about other parameters relevant to population dynamics. These included survival rate, recruitment rate, and population size. The evolution of methods for estimating population size, survival, recruitment, and movement is reviewed and we show it to be closely tied to bird-banding data. These estimation methods have been used with bird-banding data to draw important inferences about evolutionary ecology, population ecology, and population management. Illustrative examples of such inferences are provided.

Book chapter↗

Enhancing mineral systems exploration through geochronology, thermochronology, and isotope analysis: USGS Geochron and USGS Isotope databases

A mineral systems approach to mineral exploration provides a comprehensive framework for understanding ore deposit formation by examining the geodynamic, magmatic, hydrothermal, and sedimentary processes responsible for mineralization, alteration, and remobilization of economic mineral deposits. Temporal and thermal constraints on ore genesis are crucial for refining mineral system models and guiding predictive exploration strategies. Geochronology and thermochronology offer invaluable insights into the timing and thermal evolution of ore-forming processes, whereas isotopic analyses provide critical information on the source and geochemical history of ore-forming fluids. Combining these methodologies have proven highly effective for mineral exploration in regions like Australia, however, their combined application has been limited in the United States. To apply these tools to mineral systems-based exploration, the U.S. Geological Survey (USGS) has developed two products: (1) The USGS Geochron Database, and (2) the USGS Isotope Database. These databases provide centralized repositories of geo/thermochronological dates and data (Geochron Database) and both radiogenic and stable isotope data (Isotope Database) generated by the USGS and partners over the past decades. Integrating these datasets together and with traditional exploration approaches provides the mineral exploration community with powerful tools for determining the temporal and thermal histories of ore systems and identifying metallogenic source provinces.

Continental United States↗

Foundational uncertainties in terminal Ediacaran chronostratigraphy revealed by high-precision zircon U-Pb geochronology of the Nama Group, Namibia

The Nama Group of southern Namibia and northwestern South Africa hosts the best-dated mixed carbonate-siliciclastic foreland basin succession of the terminal Ediacaran [ca. 551 million years (Ma) ago to <538 Ma] and is key for resolving the chronology of early metazoan evolution. Numerous silicified volcanic tuff interbeds are present, but differing interpretations regarding the fidelity of their ages lead to different regional stratigraphic correlations, especially for the Urusis Formation of the Schwarzrand Subgroup. An expanded record of the Urusis Formation is found in the Swartpunt area of southern Namibia, which has yielded an important metazoan biota. But the succession in this area is preserved as a series of thrusts at the leading edge of the Gariep orogenic belt and zircon U-Pb data show systematic age repetition. We use regional stratigraphic and structural mapping, integrated with carbonate carbon isotope (δ 13 C carb ) chemostratigraphy and high-precision radioisotope U-Pb zircon geochronology from outcrop and recently acquired drill core to develop a temporally calibrated basin-wide depositional model. This integrated dataset either reflects complex zircon reworking, inheritance, or potential analytical biases (Scenario 1) or the presence of a Gariep-related cryptic décollement within the Spitskop Member that has resulted in stratigraphic repetition (Scenario 2). We investigate the evidence for and against both scenarios and consider their implications for stratigraphic and δ 13 C carb correlations between the Swartpunt area and coeval exposures along the Orange River border with South Africa. Given that these issues are in an area that hosts numerous silicified ash beds and extensive exposure, an inability to confidently discount either scenario highlights a level of compounding uncertainty in zircon U-Pb geochronology that must be considered when attempting to build global chronostratigraphic frameworks. Scenario 1 implies that some of the weighted mean ages and Bayesian eruption ages from the Swartpunt area may be >1 Myr older than the depositional age of their respective ash beds when assuming existing stratigraphic correlations. If this scenario is preferred, then a cautious approach would be to consider all weighted mean zircon U-Pb ages from ash beds to reflect maximum depositional ages. Both scenarios support deposition of the Huns Member >540 Ma in the Swartpunt area if the oldest weighted mean age reported here represents a near-depositional age, which has significant implications for the temporal calibration of important terminal Ediacaran ichnofossil assemblages and future cyclostratigraphic studies. Stratigraphic correlations common to both scenarios allow us to temporally calibrate a basin evolution model for the Nama Group. Temporal trends in initial hafnium isotope (εHf) compositions of zircon grains from ash beds throughout the succession may support progressive crustal thickening associated with underplating of the Damara orogenic belt along the northern periphery of the Kalahari craton from ca. 547 Ma to ca. 538 Ma. The compilation of new and published zircon U-Pb ages may also imply that the locus of carbonate platform development migrated from north to south (present co-ordinates), tracking the migration of foredeep subsidence.

Earth-Science Reviews↗

An analytical framework for estimating aquatic species density from environmental DNA

Environmental DNA (eDNA) analysis of water samples is on the brink of becoming a standard monitoring method for aquatic species. This method has improved detection rates over conventional survey methods and thus has demonstrated effectiveness for estimation of site occupancy and species distribution. The frontier of eDNA applications, however, is to infer species density. Building upon previous studies, we present and assess a modeling approach that aims at inferring animal density from eDNA. The modeling combines eDNA and animal count data from a subset of sites to estimate species density (and associated uncertainties) at other sites where only eDNA data are available. As a proof of concept, we first perform a cross-validation study using experimental data on carp in mesocosms. In these data, fish densities are known without error, which allows us to test the performance of the method with known data. We then evaluate the model using field data from a study on a stream salamander species to assess the potential of this method to work in natural settings, where density can never be known with absolute certainty. Two alternative distributions (Normal and Negative Binomial) to model variability in eDNA concentration data are assessed. Assessment based on the proof of concept data (carp) revealed that the Negative Binomial model provided much more accurate estimates than the model based on a Normal distribution, likely because eDNA data tend to be overdispersed. Greater imprecision was found when we applied the method to the field data, but the Negative Binomial model still provided useful density estimates. We call for further model development in this direction, as well as further research targeted at sampling design optimization. It will be important to assess these approaches on a broad range of study systems.

Ecology and Evolution↗

Microbiomes from biorepositories? 16S rRNA bacterial amplicon sequencing of archived and contemporary intestinal samples of wild mammals (Eulipotyphla: Soricidae)

Interest in gut microbial community composition has exploded recently as a result of the increasing ability to characterize these organisms and a growing understanding of their role in host fitness. New technologies, such as next generation amplicon (16S rRNA) sequencing, have enabled identification of bacterial communities from samples of diverse origin (e.g., fecal, skin, genital, environmental, etc.). Relatively little work, however, has explored the feasibility of utilizing historical samples (e.g., museum archived samples) of varying age, quality, and preservation type. Because natural history collections span multiple decades, these biorepositories have the potential to provide fundamental historical baselines to measure and better understand biodiversity on a changing planet. Utilizing even a small proportion of museum specimens could provide a means of sampling past microbial communities, allowing for direct comparison to contemporary communities and more complete understanding of dynamic shifts through time. We examined the feasibility of obtaining 16S rRNA amplicon microbiome data from whole gastrointestinal tracts (GIs) of shrews of varying age and preservation method, including 5 freshly collected shrew GIs immediately fixed in liquid nitrogen (LN2), 10 ten-year old shrew GIs frozen at −20°C (whole animal), and 10 shrews of varying ages (4 from 1968, 1 from 1980, 1 from 2001, 1 from 2004, 1 from 2007, 1 from 2011 and 2 from 2013) fixed and stored whole in 70% ethanol. Not surprisingly, results of 16S rDNA amplicon sequencing reveal significantly different bacterial communities between different preservation techniques and age of samples. Ten-year old frozen samples had bacterial communities most similar to freshly collected (LN2) samples, while the bacterial communities of both were significantly different from the 70% ethanol preserved samples of various ages. Amongst those preserved in 70% ethanol, age of samples also influenced bacterial community composition. Additionally, we compare results of OTU based and ASV based analyses. Looking ahead, field collectors and museums should develop and adopt best practices related to frozen preservation to ensure adequate material for future microbiome investigations.

Frontiers in Ecology and Evolution↗