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The INI North American Regional Nitrogen Center: 2011–2015 nitrogen activities in North America

The North American Nitrogen Center (NANC) carries out three main charges: (1) conducting assessments on nitrogen (N) flows within North America and the consequences for human health, water resources, biodiversity, and greenhouse gas emissions; (2) facilitating efforts to develop solutions to the problem of excess nitrogen in agricultural, institutional, and natural resource management sectors; and (3) presenting these results to policy makers. There are formidable challenges in reducing N loss from all parts of the North American food production and supply chain, including altering consumer behavior. The NANC is working with producers, trade groups, universities, and supply chains to develop effective practices for minimizing loss of reactive nitrogen (N r ) to the environment. The NANC is also helping public land management and regulatory agencies prepare effective policy approaches toward minimizing ecological damage from atmospheric N r deposition.

Book chapter

Insect pollinator crossing of international border barriers along the U.S.-Mexico border

Infrastructure along the U.S.-Mexico Border may not be equally permeable to all types of insect pollinators with potential implications for pollen and gene flow between plant populations. Pollinators were observed on their approach to two types of border barriers (slatted and cemented) along the U.S.-Mexico Border from March 2023 to January 2024. Near the barrier, four insect behaviors were observed including 1) flying over the barrier, 2) crossing through the slats of the barrier, 3) not crossing the barrier, or 4) flying parallel to the barrier without crossing. Overall, 90.2% of the pollinators crossed the barrier. Butterflies were most often observed flying over the barrier (86.8%) or sometimes moving through the slats in the barrier (6.8%). It was more common for moths to crawl through the slats than to fly over the barrier based on the occurrence model. On windy days, both butterflies and moths sometimes flew parallel to the barrier without crossing (1.2% and 27.3%, respectively), although moth crossing behavior was not related to the abundance model. Butterfly abundance increased in higher temperatures and decreased in higher wind speeds. Other insect pollinators were also observed (bee, skipper, wasp) but their crossing behavior was not significantly related to the model. Because pollinators support endangered plant species, strategies to facilitate their barrier crossing could support plant conservation in South Texas.

Texas

Implications of scale-independent habitat specialization on persistence of a rare small mammal

We assessed the habitat use patterns of the Amargosa vole Microtus californicus scirpensis , an endangered rodent endemic to wetland vegetation along a 3.5 km stretch of the Amargosa River in the Mojave Desert, USA. Our goals were to: (1) quantify the vole’s abundance, occupancy rates and habitat selection patterns along gradients of vegetation cover and spatial scale; (2) identify the processes that likely had the greatest influence on its habitat selection patterns. We trapped voles monthly in six 1 ha grids from January to May 2012 and measured habitat structure at subgrid (View the MathML source225m2) and trap (View the MathML source1m2) scales in winter and spring seasons. Regardless of scale, analyses of density, occupancy and vegetation structure consistently indicated that voles occurred in patches of bulrush (Schoenoplectus americanus ; Cyperaceae) where cover >50%. The majority of evidence indicates the vole's habitat selectivity is likely driven by bulrush providing protection from intense predation. However, a combination of selective habitat use and limited movement resulted in a high proportion of apparently suitable bulrush patches being unoccupied. This suggests the Amargosa vole's habitat selection behavior confers individual benefits but may not allow the overall population to persist in a changing environment.

Global Ecology and Conservation

Movement patterns and spatial segregation of two populations of lake trout Salvelinus namaycush in Lake Huron

Movement ecology is an important component of life history and population dynamics, and consequently its understanding can inform successful fishery management decision-making. While lake trout populations in Lake Huron have shown signs of recovery from near extinction in recent years, knowledge of their movement behavior remains incomplete. We used acoustic telemetry to describe and compare movement patterns of two Lake Huron lake trout populations: Drummond Island and Thunder Bay. Both populations showed high spawning site fidelity, with no evidence of co-mingling during non-spawning season. Detections between spawning periods were mainly limited to receivers within 100 km of spawning locations, and suggested that the two populations likely remained segregated throughout the year. Drummond Island fish, which spawn inside the Drummond Island Refuge, primarily dispersed east into Canadian waters of Lake Huron, with 79–92% of fish being detected annually on receivers outside the refuge. In contrast, Thunder Bay fish tended to disperse south towards Saginaw Bay. Large proportions (i.e., > 80%) of both populations were available to fisheries outside the management zone containing their spawning location. Thunder Bay fish moved relatively quickly to overwinter habitat after spawning, and tended to repeat the same post-spawning movement behavior each year. The consistent, predictable movement of both populations across management zones highlights the importance of understanding population dynamics to effective management of Lake Huron lake trout.

Journal of Great Lakes Research

Change-point models for identifying behavioral transitions in wild animals

Animal behavior can be difficult, time-consuming, and costly to observe in the field directly. Innovative modeling methods, such as hidden Markov models (HMMs), allow researchers to infer unobserved animal behaviors from movement data, and implementations often assume that transitions between states occur multiple times. However, some behavioral shifts of interest, such as parturition, migration initiation, and juvenile dispersal, may only occur once during an observation period, and HMMs may not be the best approach to identify these changes. We present two change-point models for identifying single transitions in movement behavior: a location-based change-point model and a movement metric-based change-point model. We first conducted a simulation study to determine the ability of these models to detect a behavioral transition given different amounts of data and the degree of behavioral shifts. We then applied our models to two ungulate species in central Pennsylvania that were fitted with global positioning system collars and vaginal implant transmitters to test hypotheses related to parturition behavior. We fit these models in a Bayesian framework and directly compared the ability of each model to describe the parturition behavior across species. Our simulation study demonstrated that successful change point estimation using either model was possible given at least 12 h of post-change observations and 15 min fix interval. However, our models received mixed support among deer and elk in Pennsylvania due to behavioral variation between species and among individuals. Our results demonstrate that when the behavior follows the dynamics proposed by the two models, researchers can identify the timing of a behavioral change. Although we refer to detecting parturition events, our results can be applied to any behavior that results in a single change in time.

Movement Ecology

Mule deer (Odocoileus hemionus) resource selection: Trade-offs between forage and predation risk

Ungulates commonly select habitat with higher forage biomass and or nutritional quality to improve body condition and fitness. However, predation risk can alter ungulate habitat selection and foraging behavior and may affect their nutritional condition. Ungulates often choose areas with lower predation risk, sometimes sacrificing higher quality forage. This forage–predation risk trade-off can be important for life history strategies and influences individual nutritional condition and population vital rates. We used GPS collar data from adult female mule deer ( Odocoileus hemionus ) and mountain lions ( Puma concolor ) to model mule deer habitat selection in relation to forage conditions, stalking cover and predation risk from mountain lions to determine if a forage-predation risk trade-off existed for mule deer in central New Mexico. We also examined mountain lion kill sites and mule deer foraging locations to assess trade-offs at a finer scale. Forage biomass and protein content were inversely correlated with horizontal visibility, hence associated with higher stalking cover for mountain lions, suggesting a forage-predation risk trade-off for mule deer. Mule deer habitat selection was influenced by forage biomass and protein content at the landscape and within home range spatial scales, with forage protein being related to habitat selection during spring and summer and forage biomass during winter. However, mule deer selection for areas with better foraging conditions was constrained by landscape-scale encounter risk for mountain lions, such that increasing encounter risk was associated with diminished selection for areas with better foraging conditions. Mule deer also selected for areas with higher visibility when mountain lion predation risk was higher. Mountain lion kill sites were best explained by decreasing horizontal visibility and available forage protein, suggesting that deer may be selecting for forage quality at the cost of predation risk. A site was 1.5 times more likely to be a kill site with each 1-meter decrease in visibility (i.e., increased stalking cover). Mule deer selection of foraging sites was related to increased forage biomass, further supporting the potential for a trade-off scenario. Mule deer utilized spatio-temporal strategies and risk-conditional behavior to reduce predation risk, and at times selected suboptimal foraging areas with lower predation risk.

New Mexico

Intrinsic prey preference and selection of the giant gartersnake: A threatened predator in a nonnative prey-dominated community

The introduction of exotic species into an environment can introduce great change in the trophic dynamics of native species. This is of even greater concern if the native species is of conservation concern. The giant gartersnake, Thamnophis gigas , an endemic predator of the Central Valley of California and a species of conservation concern at the state and federal levels, has declined as a result of conversion of its once vast wetland habitat to agriculture. Another anthropogenic factor contributing to this snake's changing ecology is the introduction of nonnative prey into the species' habitats. These introductions have resulted in a prey community that is almost completely composed of exotic species and have potential for considerable effects. In order to assess prey preference and selection we performed three sets of behavioral trials on naïve neonates. We examined 1) neonate prey preference in response to olfactory cues of prepared prey extracts, 2) neonate consumption of different live prey items presented simultaneously; and 3) terrestrial feeding behavior and/or latency to successful attack. Results from the olfactory study suggest that native Sierran treefrogs, Pseudacris sierra, are preferred by neonates. Results from consumption trials suggest that neonates are more likely to select frog species than fish species. This is the first study that we are aware of that examines prey selection of this threatened species and serves to inform its conservation and management.

Journal of Fish and Wildlife Management

Assessing peridomestic entomological factors as predictors for Lyme disease

The roles of entomologic risk factors, including density of nymphal blacklegged ticks ( Ixodes scapularis ), prevalence of nymphal infection with the etiologic agent ( Borrelia burgdorferi) , and density of infected nymphs, in determining the risk of human Lyme disease were assessed at residences in the endemic community of South Kingstown, RI. Nymphs were sampled between May and July from the wooded edge around 51 and 47 residential properties in 2002 and 2003, respectively. Nymphs were collected from all residences sampled. Tick densities, infection rates, and densities of infected nymphs were all significantly higher around homes reporting Lyme disease histories in 2003, while only infection rates were significantly higher in 2002. However, densities of infected nymphs did not significantly predict the probability of Lyme disease at a residence (by logistic regression) in either year. There were no significant differences in entomologic risk factors between homes with state-confirmed Lyme disease histories and homes with self-reported cases (not reported to the state health department). Therefore, although entomologic risk factors tended to be higher at residences with cases of Lyme disease, entomological indices, in the absence of human behavior measures, were not useful predictors of Lyme disease at the scale of individual residences in a tick-endemic community.

Rhode Island

Thermal effects on fish ecology

Of all the environmental factors that influence aquatic organisms, temperature is the most all-pervasive. There is always an environmental temperature while other factors may or may not be present to exert their effects. Fish are, for all practical purposes, thermal conformers, or obligate poikilotherms. That is, they are able to exert little significant influence on maintaining a certain body temperature by specialized metabolic or behavioral means. Their body temperature thus fluctuates nearly in concert with the temperature of their aquatic medium (although particularly large, actively-moving fish such as tuna have deep muscle temperatures slightly higher than the water). Intimate contact at the gills of body fluids with the outside water and the high specific heat of water provide a very efficient heat exchanger that insures this near identity of internal and external temperatures.

Book chapter

The first documented interaction between a long-tailed weasel (Mustela frenata) and a plains spotted skunk (Spilogale interrupta) carcass

A novel interaction between a long-tailed weasel ( Mustela frenata ) and a plains spotted skunk ( Spilogale interrupta ) carcass is detailed. In November 2020, a farmer in Edmunds County in north-central South Dakota sent in a video recording of a long-tailed weasel with a spotted skunk carcass. Location of the event, carcass condition, and recorded behavior of the long-tailed weasel offer probable, but unconfirmed, evidence that the spotted skunk was killed by the long-tailed weasel.

South Dakota

Nutrient controls on biocomplexity of mangrove ecosystems

Mangrove forests are important coastal ecosystems that provide a variety of ecological and societal services. These intertidal, tree-dominated communities along tropical coastlines are often described as “simple systems,” compared to other tropical forests with larger numbers of plant species and multiple understory strata; however, mangrove ecosystems have complex trophic structures, and organisms exhibit unique physiological, morphological, and behavioral adaptations to environmental conditions characteristic of the land-sea interface. Biogeochemical functioning of mangrove forests is also controlled by interactions among the microbial, plant, and animal communities and feedback linkages mediated by hydrology and other forcing functions. Scientists with the U.S. Geological Survey (USGS) at the National Wetlands Research Center are working to understand more fully the impact of nutrient variability on these delicate and important ecosystems.

Fact Sheet

Parasites in marine food webs

Most species interactions probably involve parasites. This review considers the extent to which marine ecologists should consider parasites to fully understand marine communities. Parasites are influential parts of food webs in estuaries, temperate reefs, and coral reefs, but their ecological importance is seldom recognized. Though difficult to observe, parasites can have substantial biomass, and they can be just as common as free-living consumers after controlling for body mass and trophic level. Parasites have direct impacts on the energetics of their hosts and some affect host behaviors, with ecosystem-level consequences. Although they cause disease, parasites are sensitive components of ecosystems. In particular, they suffer secondary extinctions due to biodiversity loss. Some parasites can also return to a system after habitat restoration. For these reasons, parasites can make good indicators of ecosystem integrity. Fishing can indirectly increase or decrease parasite populations and the effects of climate change on parasites are likely to be equally as complex.

Bulletin of Marine Science

Distribution, migration behavior, habitat use, and species interactions of fall-released juvenile hatchery spring Chinook salmon in the Deschutes River, Oregon, 2003

In a review of National Fish Hatcheries (NFH), the U.S. Fish and Wildlife Service (USFWS) identified the need to assess the fate of hatchery-reared fish and their potential effect on the aquatic community (USFWS 1998). Additionally, the National Marine Fisheries Service (NMFS) recommended monitoring and evaluating ecological interactions between hatchery and wild fish (NMFS 1999; Columbia River Biological Opinion). In 2003, a study was designed to investigate the fate of hatchery-reared fish and to assess habitat use and fish interactions in the Deschutes River, Oregon.

Oregon

A landscape model of variable social-ecological fire regimes

Fire regimes are now recognized as the product of social processes whereby fire on any landscape is the product of human-generated drivers: climate change, historical patterns of vegetation manipulation, invasive species, active fire suppression, ongoing fuel management efforts, prescribed burning, and accidental ignitions. We developed a new fire model (Social-Climate Related Pyrogenic Processes and their Landscape Effects: SCRPPLE) that emphasizes the social dimensions of fire and enables simulation of fuel-treatment effects, fire suppression, and prescribed fires. Fire behavior was parameterized with daily fire weather, ignition, and fire-boundary data. SCRPPLE was initially parameterized and developed for the Lake Tahoe Basin (LTB) in California and Nevada, USA although its behavior is general and could be applied worldwide. We demonstrate the behavior and utility of our model via four simple scenarios that emphasize the social dimensions of fire regimes: a) Recent Historical: simulated recent historical patterns of lightning and accidental fires and current patterns of fire suppression, b) Natural-Fire-Regime: simulated wildfire without suppression, accidental fires, or prescribed fires, holding all other factors the same as Recent Historical, c) Enhanced Suppression: simulated a doubling of the effectiveness of suppression, holding all other factors the same as Recent Historical, and d) Reduced Accidental Ignitions: within which the number of accidental fires was reduced by half, holding all other factors the same as Recent Historical. Results indicate that SCRPPLE can recreate past fire regimes, including size, intensity, and locations. Furthermore, our results indicate that the ‘Enhanced Suppression’ and ‘Reduced Accidental Ignitions’ scenarios had similar capacity to reduce fire and related tree mortality over time, suggesting that within the broad outlines of the scenarios, reducing accidental fires can be as effective as substantially increasing resources for suppression.

California, Nevada

Genetic implications of bottleneck effects of differing severities on genetic diversity in naturally recovering populations: An example from Hawaiian coot and Hawaiian gallinule

The evolutionary trajectory of populations through time is influenced by the interplay of forces (biological, evolutionary, and anthropogenic) acting on the standing genetic variation. We used microsatellite and mitochondrial loci to examine the influence of population declines, of varying severity, on genetic diversity within two Hawaiian endemic waterbirds, the Hawaiian coot and Hawaiian gallinule, by comparing historical (samples collected in the late 1800s and early 1900s) and modern (collected in 2012–2013) populations. Population declines simultaneously experienced by Hawaiian coots and Hawaiian gallinules differentially shaped the evolutionary trajectory of these two populations. Within Hawaiian coot, large reductions (between −38.4% and −51.4%) in mitochondrial diversity were observed, although minimal differences were observed in the distribution of allelic and haplotypic frequencies between sampled time periods. Conversely, for Hawaiian gallinule, allelic frequencies were strongly differentiated between time periods, signatures of a genetic bottleneck were detected, and biases in means of the effective population size were observed at microsatellite loci. The strength of the decline appears to have had a greater influence on genetic diversity within Hawaiian gallinule than Hawaiian coot, coincident with the reduction in census size. These species exhibit similar life history characteristics and generation times; therefore, we hypothesize that differences in behavior and colonization history are likely playing a large role in how allelic and haplotypic frequencies are being shaped through time. Furthermore, differences in patterns of genetic diversity within Hawaiian coot and Hawaiian gallinule highlight the influence of demographic and evolutionary processes in shaping how species respond genetically to ecological stressors.

Ecology and Evolution

Macroscale patterns of synchrony identify complex relationships among spatial and temporal ecosystem drivers

Ecology has a rich history of studying ecosystem dynamics across time and space that has been motivated by both practical management needs and the need to develop basic ideas about pattern and process in nature. In situations in which both spatial and temporal observations are available, similarities in temporal behavior among sites (i.e., synchrony) provide a means of understanding underlying processes that create patterns over space and time. We used pattern analysis algorithms and data spanning 22–25 yr from 601 lakes to ask three questions: What are the temporal patterns of lake water clarity at sub‐continental scales? What are the spatial patterns (i.e., geography) of synchrony for lake water clarity? And, what are the drivers of spatial and temporal patterns in lake water clarity? We found that the synchrony of water clarity among lakes is not spatially structured at sub‐continental scales. Our results also provide strong evidence that the drivers related to spatial patterns in water clarity are not related to the temporal patterns of water clarity. This analysis of long‐term patterns of water clarity and possible drivers contributes to understanding of broad‐scale spatial patterns in the geography of synchrony and complex relationships between spatial and temporal patterns across ecosystems.

Ecosphere

Simulating cholinesterase inhibition in birds caused by dietary insecticide exposure

We describe a stochastic simulation model that simulates avian foraging in an agricultural landscape to evaluate factors affecting dietary insecticide exposure and to predict post-exposure cholinesterase (ChE) inhibition. To evaluate the model, we simulated published field studies and found that model predictions of insecticide decay and ChE inhibition reasonably approximated most observed results. Sensitivity analysis suggested that foraging location usually influenced ChE inhibition more than diet preferences or daily intake rate. Although organophosphorus insecticides usually caused greater inhibition than carbamate insecticides, insecticide toxicity appeared only moderately important. When we simulated impact of heavy insecticide applications during breeding seasons of 15 wild bird species, mean maximum ChE inhibition in most species exceeded 20% at some point. At this level of inhibition, birds may experience nausea and/or may exhibit minor behavioral changes. Simulated risk peaked in April–May and August–September and was lowest in July. ChE inhibition increased with proportion of vegetation in the diet. This model, and ones like it, may help predict insecticide exposure of and sublethal ChE inhibition in grassland animals, thereby reducing dependence of ecological risk assessments on field studies alone.

Ecological Modelling

Multi-decadal trophic shifts in Lake Erie yellow perch Perca flavescens

In Lake Erie, yellow perch Perca flavescens support vast commercial and recreational fisheries, yet populations have recently declined. Using N = 5889 yellow perch stomachs collected from 1997 to 2021, we explored trends in the feeding ecology and trophic level of yellow perch with generalized additive models. Models revealed a significant decrease in yellow perch trophic level (−0.15 trophic levels in the last decade), and significant dietary shifts. Yellow perch have shifted away from feeding on piscine prey and round goby Neogobius melanostomus over the 25-year period, and now feed on invertebrates more frequently—including invasive waterfleas ( Bythotrephes longimanus and Cercopagis pengoi ) and chironomids. Dietary patterns appear to reflect broad ecological changes—invasive waterfleas have proliferated while populations of forage fish and round goby have declined. Furthermore, hypoxia events have increased in duration and severity, which may explain observed increases in chironomid consumption, which are hypoxia tolerant. This study demonstrates trophic adaptability in yellow perch, which have changed feeding behavior and trophic position in response to novel invaders and changing environmental conditions.

Lake Erie