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Effect of treatment in a constructed wetland on toxicity of textile wastewater

Constructed wetlands for treating wastewater have proliferated in recent years and their characteristics have been studied extensively. In most cases, constructed wetlands have been used primarily for removal of nutrients and heavy metals. Extensive literature is available concerning construction and use of wetlands for treatment of wastewater. Even so, quantitative descriptions of wetland function and processes are highly empirical and difficult to extrapolate. The processes involved in removal of pollutants by wetlands are poorly understood, especially for waste streams as complex as textile effluents. The few studies conducted on treatment of textile wastewater in constructed wetlands were cited in earlier publications. Results of a two-year study of a full-scale wetland treating textile effluent are presented here. The paper describes the effects of the wetland on aquatic toxicity of the wastewater and draws conclusions about the utility and limitations of constructed wetlands for treatment of textile effluents.

AATCC Review↗

Tests of rhodamine WT dye for toxicity to oysters and fish

Because of the toxicity to oyster larvae and eggs of rhodamine B dye in concentrations greater than 1 mg/l in earlier tests, there was a concern that rhodamine WT, a similar tracer dye, would have a detrimental effect on marine life being developed under the aquaculture program of the Lummi Indian Tribe near Bellingham, Wash. Tests showed that 48-hour exposures at 24° C of 11,000 oyster eggs per liter and 6,000 12-day-old larvae per liter, in sea water with concentrations of rhodamine WT ranging from 1 μg/l to 10 mg/l, resulted in development of the eggs to normal straight-hinge larvae and no abnormalities in the larvae development. Tests made on the smolt of both silver salmon and Donaldson trout, with the fish held for 17.5 hours in a tankfull of sea water with a dye concentration of 10 mg/l at 22°C showed no mortalities or respiratory problems. With the concentration increased to 375 mg/l, and the time extended an additional 3.2 hours, still no mortalities or abnormalities were noted. The fish remained healthy in dye-free water when last checked a month after the test.

Journal of Research of the U.S. Geological Survey↗

Alternative aircraft anti-icing formulations with reduced aquatic toxicity and biochemical oxygen demand

The current research was conducted to identify alternative aircraft and pavement deicer and anti-icer formulations with improved environmental characteristics compared to currently used commercial products (2007). The environmental characteristics of primary concern are the biochemical oxygen demand (BOD) and aquatic toxicity of the fully formulated products. Except when the distinction among products is necessary for clarity, “deicer” will refer to aircraft-deicing fluids (ADFs), aircraft anti-icing fluids (AAFs), and pavementdeicing materials (PDMs).

Report↗

Infection by Nanophyetus salmincola and toxic contaminant exposure in out‐migrating steelhead from Puget Sound, Washington: Implications for early marine survival

Out‐migrating steelhead Oncorhynchus mykiss from four Puget Sound rivers and associated marine basins of Puget Sound in Washington State were examined for the parasite, Nanophyetus salmincola in 2014 to determine whether recent trends in reduced marine survival are associated with the presence of this pathogen. A subset of steelhead from three of these river–marine basin combinations was analyzed for the presence of persistent organic pollutants (POPs) to assess whether exposure to these contaminants is a contributing factor to their reduced marine survival. The prevalence and parasite load of N. salmincola were significantly higher in fish from central and southern Puget Sound than in fish from river systems in northern Puget Sound. The proportion of steelhead samples with concentrations of POPs higher than adverse effects thresholds (AETs) or concentrations known to cause adverse effects was also greater in fish from the central and southern regions of Puget Sound than in those from the northern region. Polybrominated diphenyl ether concentrations associated with increased disease susceptibility were observed in 10% and 40% of the steelhead sampled from central and southern Puget Sound regions, respectively, but in none of the fish sampled from the northern region. The AET for polychlorinated biphenyls was exceeded in steelhead collected from marine habitats: 25% of the samples from the marine basins in the central and southern regions of Puget Sound and 17% of samples from northern Puget Sound region. Both N. salmincola and POP levels suggest there are adverse health effects on out‐migrating steelhead from one southern and one central Puget Sound river that have lower early marine survival than those from a river system in northern Puget Sound.

Washington↗

Toxicity of elevated partial pressures of carbon dioxide to invasive New Zealand mudsnails

The authors tested the efficacy of elevated partial pressures of CO 2 to kill invasive New Zealand mudsnails. The New Zealand mudsnails were exposed to 100 kPa at three water temperatures, and the survival was modeled versus dose as cumulative °C-h. We estimated an LD50 of 59.4°C-h for adult and juvenile New Zealand mudsnails. The results suggest that CO 2 may be an effective and inexpensive lethal tool to treat substrates, tanks, or materials infested with New Zealand mudsnails.

Environmental Toxicology and Chemistry↗

Evaluation of the short term 12 hour toxicity of 3-trifluoromethyl-4-nitrophenol (TFM) to multiple life stages of Venustaconcha ellipsiformis and Epioblasma triquetra and its host fish ( Percina caprodes )

The present study evaluated the risk of 12-h exposures of the lampricide 3-trifluoromethyl-4-nitrophenol (TFM) to multiple life stages of the federally endangered snuffbox ( Epioblasma triquetra ) and its primary host fish the common logperch ( Percina caprodes ) as well as a surrogate to the snuffbox, the ellipse ( Venustaconcha ellipsiformis ). Life stages examined included free glochidia, 1-wk juveniles, and adults of the ellipse; free glochidia, glochidia on host fish, and 1-wk juveniles of the snuffbox; and adult logperch. Larval sea lampreys were also tested alongside adult ellipse and logperch for direct comparison. Survival exceeded 82% among all life stages in both mussel species at levels up to 1.8 times what would be applied during treatments, suggesting that routine sea lamprey control operations would not adversely affect mussels. However, substantial mortality of adult logperch was observed at TFM concentrations typically applied to streams, and loss of host fish could adversely affect snuffbox reproduction. In addition, TFM had no significant effect on the number of glochidia that metamorphosed on adult logperch. Although the snuffbox is not likely to be acutely affected from sea lamprey control operations, mitigation efforts to minimize impacts to the host fish should be considered.

Environmental Toxicology and Chemistry↗

Toxic effects of dietary methylmercury on immune function and hematology in American kestrels (Falco sparverius)

Fifty-nine adult male American kestrels ( Falco sparverius ) were assigned to one of three diet formulations including 0 (control), 0.6, and 3.9 &mu;g/g (dry wt) methylmercury (MeHg). Kestrels received their diets daily for 13 weeks to assess the effects of dietary MeHg on immunocompetence. Immunotoxic endpoints included assessment of cell-mediated immunity (CMI) using the phytohemagglutinin (PHA) skin-swelling assay and primary and secondary antibody-mediated immune responses (IR) via the sheep red blood cell (SRBC) hemagglutination assay. Select hematology and histology parameters were evaluated to corroborate the results of functional assays and to assess immunosuppression of T and B cell-dependent components in spleen tissue. Kestrels in the 0.6 and 3.9 &mu;g/g MeHg groups exhibited suppression of CMI, including lower PHA stimulation indexes ( p = 0.019) and a 42 to 45% depletion of T cell-dependent splenic lymphoid tissue ( p = 0.006). Kestrels in the 0.6 &mu;g/g group exhibited suppression of the primary IR to SRBCs ( p = 0.014). MeHg did not have a noticeable effect on the secondary IR ( p = 0.166). Elevation of absolute heterophil counts ( p < 0.001), the heterophil to lymphocyte ratio ( p < 0.001), and total white blood cell counts ( p = 0.003) was apparent in the 3.9 &mu;g/g group at week 12. Heterophilia, or the excess of heterophils in peripheral blood above normal ranges, was apparent in seven of 17 (41%) kestrels in the 3.9 &mu;g/g group and was indicative of an acute inflammatory response or physiological stress. This study revealed that adult kestrels were more sensitive to immunotoxic effects of MeHg at environmentally relevant dietary concentrations than they were to reproductive effects as previously reported.

Environmental Toxicology and Chemistry↗

Acute toxicity of diazinon is similar for eight stocks of bobwhite

Nine-week-old bobwhite ( Colinus virginianus ) from eight different game farms were tested for their sensitivity to an acute oral exposure of technical-grade diazinon (phosphorothioic acid O,O -diethyl- O -[6-methyl-2-(l-methylethyl) -4-pyrimidinyl]ester). Extraneous variables associated with interlaboratory differences in husbandry were eliminated by incubating eggs and rearing chicks to test age for all stocks simultaneously in the same facilities at the Patuxent Wildlife Research Center. Under this single set of conditions, the responses of the eight stocks of bobwhite to diazinon were statistically inseparable, with LD 50 values varying from 13 mg/kg (95% confidence interval, 8–21 mg/kg) to 17 mg/kg (95% confidence interval, 11–25 mg/kg). The pooled LD 50 for the eight stocks was 14.7 mg/kg (95% confidence interval, 13.1–16.5 mg/kg).

Environmental Toxicology and Chemistry↗

Acute toxicity of four anticholinesterase insecticides to American kestrels, eastern screech-owls and northern bobwhites

American kestrels (Falco sparverius), eastern screech-owls (Otus asio), and northern bobwhites (Colinus virginianus) were given single acute oral doses of four widely diverse anticholinesterase pesticides: EPN, fenthion, carbofuran, and monocrotophos. LD50s, based on birds that died within 5 d of dosage, were computed for each chemical in each species. Sex differences in the sensitivity of northern bobwhites in reproductive condition were examined. American kestrels were highly sensitive to all chemicals tested (LD50s 0.6--4.0 mg/kg). Eastern screech-owls were highly tolerant to EPN (LD50 274 mg/kg) but sensitive to the remaining chemicals (LD50s 1.5-3.9 mg/kg). Northern bobwhites were highly sensitive to monocrotophos (LD50 0.8 mg/kg) and less sensitive to the remaining chemicals (LD50s 4.6--31 mg/kg). Female bobwhites (LD50 3.1 mg/kg) were more sensitive to fenthion than males (LD50 7.0 mg/kg). Mean percent depression of brain cho[inesterase (ChE) of birds that died on the day of dosing exceeded 65% for all chemicals in all species. The response of one species to a given pesticide should not be used to predict the sensitivity of other species to the same pesticide. The need for research on several topics is discussed

Environmental Toxicology and Chemistry↗

Evaluation of peroxidase as a biochemical indicator of toxic chemical exposure in the aquatic plant Hydrilla verticillata, Royle

Laboratory bioassays were conducted to determine the utility of peroxidase (POD) activity as a biochemical indicator of contaminant exposure in the aquatic plant Hydrilla verticillata , Royle. The plants were exposed to anthracene, sulfomet-uron methyl (Oust®), Cd 2+ , Cr 6+ , Cu 2+ , Mn 2+ , and Se 4+ in concentration factors of 10. POD was extracted and measured by spectrophotometric assay. There was a significant increase in POD activity after a 5-d exposure to each of the chemicals at 1 mg/L. The optimum pH for POD activity after exposure to the chemicals was 5.5 to 6.0. The increase in POD was found to be dose dependent for each of the chemicals. The lowest concentration of chemical to induce a significant POD increase was 0.01 mg/L for anthracene, Oust, Cd, Cr, and Cu; 0.1 mg/L for Se; and 1.0 mg/L for Mn.

Environmental Toxicology and Chemistry↗

Toxicity of wildland fire retardants to rainbow trout in short exposures

Long-term wildland fire retardants are one important tool used to control and suppress wildfires. During suppression activities, these retardants may enter waterbodies; thus, there is a need to understand their potential effects to aquatic biota. We investigated the effect of three current-use wildland fire retardants to juvenile rainbow trout ( Oncorhynchus mykiss) survival in short exposures more realistic to actual intrusion scenarios. Lethal effect concentrations decreased with time and varied among chemicals (LC95A-R > 259-Fx > MVP-Fx). The lowest effect concentrations observed were 2 to 10 times above the threshold used by federal agencies to assess potential impacts to aquatic organisms following a retardant intrusion. These data can be used by resource managers to balance wildfire control with potential environmental impacts of retardant use.

Environmental Toxicology and Chemistry↗

Carbon dioxide toxicity to zebra mussels (Dreissena polymorpha) is dependent on water chemistry

Carbon dioxide (CO 2 ) is gaining interest as a tool to combat aquatic invasive species, including zebra mussels ( Dreissena polymorpha ). However, the effects of water chemistry on CO 2 efficacy are not well described. We conducted five trials in which we exposed adult zebra mussels to a range of CO 2 in water with adjusted total hardness and specific conductance. We compared dose–responses and found differences in lethal concentration to 50% of organisms (LC50) estimates ranging from 108.3 to 179.3 mg/L CO 2 and lethal concentration to 90% of organisms (LC90) estimates ranging from 163.7 to 216.6 mg/L CO 2 . We modeled LC50 and LC90 estimates with measured water chemistry variables from the trials. We found sodium (Na + ) concentration to have the strongest correlation to changes in the LC50 and specific conductance to have the strongest correlation to changes in the LC90. Our results identify water chemistry as an important factor in considering efficacious CO 2 concentrations for zebra mussel control. Additional research into the physiological responses of zebra mussels exposed to CO 2 may be warranted to further explain mode of action and reported selectivity. Further study could likely develop a robust and relevant model to refine CO 2 applications for a wider range of water chemistries. Environ Toxicol Chem 2024;00:1–8. Published 2024. This article is a U.S. Government work and is in the public domain in the USA. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Environmental Toxicology and Chemistry↗

Freshwater molluscs as indicators of bioavailability and toxicity of metals in surface-water systems

During the past several decades, studies from a variety of locations have demonstrated widespread occurrence of metals in surface waters at concentrations significantly higher than background levels. Elevated concentrations are not limited to certain water types or polluted areas; they appear in all types of systems and in all geographic areas. It is clear that metals enter the aquatic systems from diverse sources, both point and nonpoint, and they can be readily transported from one system to another. Transport routes include atmospheric, terrestrial, subterranean, aquatic, and biological pathways (Elder 1988; Salomons and Forstner 1984).

Book chapter↗