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Toxoplasmosis

Toxoplasmosis ( Toxoplasma gondii ), one of the better known and more widespread zoonotic diseases, originated in wildlife species and is now well established as a human malady. Food- and waterborne zoonoses, such as toxoplasmosis, are receiving increasing attention as components of disease emergence and resurgence. Toxoplasmosis is transmitted to humans via consumption of contaminated food or water, and nearly one-third of humanity has been exposed to this parasite. The role of wildlife in this transmission process is becoming more clearly known and is outlined in this report. This zoonotic disease also causes problems in wildlife species across the globe. Future generations of humans will continue to be jeopardized by toxoplasmosis infections in addition to many of the other zoonotic diseases that have emerged during the past century. Through monitoring toxoplasmosis infection levels in wildlife populations, we will be better able to predict future human infection levels of this important zoonotic disease.

Circular

The heliothermic lake: a direct method of collecting and storing solar energy

Heliothermic lakes contain a sun-heated layer of warm, saline water beneath a surface layer of cooler, less saline water. The two layers are separated by a chemocline, a stratum in which salinity increases progressively with depth. The chemocline, the position of which varies from lake to lake, functions as a heat trap. Most sunlight that penetrates this stratum is transformed into heat, which cannot escape by radiation because water is opaque to infrared light, and which cannot escape by convection because the specific gravity of the dense water below the chemocline is not significantly decreased by the increasing temperature. Heat can escape only by conduction through the chemocline, and water or brine is a very poor conductor. As a result, the temperature within and commonly below the chemocline rises. Under ideal conditions of a clear solution, high isolation, and a suitable salinity distribution, the temperature of the chemocline will increase to the boiling point. The lower part of the chemocline in a shallow (0.8-m) manmade heliothermic lake at Sedom, Israel, for example, reached a temperature of 96°C (205°F) in spite of a brine with poor light transmissibility. About 30 natural heliothermic lakes have been reported. The best known, Lake Ursului, occurs in Transylvania, Romania (latitude, 46°35'N). During four consecutive summers, 1899 to 1902, this lake had temperatures of 60-70°C (140-158°F) at a depth of 1-2 m. Heliothermic conditions have persisted in this lake for at least 28 and probably for more than 77 years. The most unusual, Lake Vanda, Victoria Land, Antarctica (latitude, 77°35'S), has a temperature of 26°C near the base of the chemocline at a depth of 61 despite a mean atmospheric temperature of -20°C. Sunlight penetrates into the chemocline through 5 m of remarkably clear ice. Maintenance of the chemocline is the chief problem preventing commercial use of manmade heliothermic lakes for the collection and storage of solar energy. The most effective means of preserving this stratum from destruction by diffusion and wind mixing may be the use of salts, such as sodium sulfate and sodium borate, whose solubilities are markedly influenced by temperature. The chemoclines of ponds constructed with such salts, in theory, would persist indefinitely and could be of great size.

Open-File Report

A Servicewide Benthic Mapping Program for National Parks

In 2007, the National Park Service (NPS) Inventory and Monitoring Program directed the initiation of a benthic habitat mapping program in ocean and coastal parks in alignment with the NPS Ocean Park Stewardship 2007-2008 Action Plan. With 74 ocean and Great Lakes parks stretching over more than 5,000 miles of coastline across 26 States and territories, this Servicewide Benthic Mapping Program (SBMP) is essential. This program will deliver benthic habitat maps and their associated inventory reports to NPS managers in a consistent, servicewide format to support informed management and protection of 3 million acres of submerged National Park System natural and cultural resources. The NPS and the U.S. Geological Survey (USGS) convened a workshop June 3-5, 2008, in Lakewood, Colo., to discuss the goals and develop the design of the NPS SBMP with an assembly of experts (Moses and others, 2010) who identified park needs and suggested best practices for inventory and mapping of bathymetry, benthic cover, geology, geomorphology, and some water-column properties. The recommended SBMP protocols include servicewide standards (such as gap analysis, minimum accuracy, final products) as well as standards that can be adapted to fit network and park unit needs (for example, minimum mapping unit, mapping priorities). SBMP Mapping Process. The SBMP calls for a multi-step mapping process for each park, beginning with a gap assessment and data mining to determine data resources and needs. An interagency announcement of intent to acquire new data will provide opportunities to leverage partnerships. Prior to new data acquisition, all involved parties should be included in a scoping meeting held at network scale. Data collection will be followed by processing and interpretation, and finally expert review and publication. After publication, all digital materials will be archived in a common format. SBMP Classification Scheme. The SBMP will map using the Coastal and Marine Ecological Classification Standard (CMECS) that is being modified to include all NPS needs, such as lacustrine ecosystems and submerged cultural resources. CMECS Version III (Madden and others, 2010) includes components for water column, biotic cover, surface geology, sub-benthic, and geoform. SBMP Data Archiving. The SBMP calls for the storage of all raw data and final products in common-use data formats. The concept of 'collect once, use often' is essential to efficient use of mapping resources. Data should also be shared with other agencies and the public through various digital clearing houses, such as Geospatial One-Stop (http://gos2.geodata.gov/wps/portal/gos). To be most useful for managing submerged resources, the SBMP advocates the inventory and mapping of the five components of marine ecosystems: surface geology, biotic cover, geoform, sub-benthic, and water column. A complete benthic inventory of a park would include maps of bathymetry and the five components of CMECS. The completion of mapping for any set of components, such as bathymetry and surface geology, or a particular theme (for example, submerged aquatic vegetation) should also include a printed report.

Open-File Report

Pharmaceuticals, hormones, pesticides, and other bioactive contaminants in water, sediment, and tissue from Rocky Mountain National Park, 2012–2013

Pharmaceuticals, hormones, pesticides, and other bioactive contaminants (BCs) are commonly detected in surface water and bed sediment in urban and suburban areas, but these contaminants are understudied in remote locations. In Rocky Mountain National Park (RMNP), Colorado, USA, BCs may threaten the reproductive success and survival of native aquatic species, benthic communities, and pelagic food webs. In 2012–2013, 67 water, 57 sediment, 63 fish, 10 frog, and 12 quality-control samples (8 water and 4 sediment) were collected from 20 sites in RMNP. Samples were analyzed for 369 parameters including 149 pharmaceuticals, 22 hormones, 137 pesticides, and 61 other chemicals or conditions to provide a representative assessment of BC occurrence within RMNP. Results indicate that BCs were detected in water and/or sediment from both remote and more accessible locations in RMNP. The most commonly detected BCs in water were caffeine, camphor, para-cresol, and DEET; and the most commonly detected BCs in sediment were indole, 3-methyl-1H-indole, para-cresol, and 2,6-dimethyl-naphthalene. Some detected contaminants, including carbaryl, caffeine, and oxycodone, are clearly attributable to direct local human input, whereas others may be transported into the park atmospherically (e.g., atrazine) or have local natural sources (e.g., para-cresol). One or more pharmaceuticals were detected in at least 1 sample from 15 of 20 sites. Most of the 29 detected pharmaceuticals are excreted primarily in human urine, not feces. Elevated net estrogenicity was observed in 18% of water samples, and elevated vitellogenin in blood was observed in 12% of male trout, both evidence of potential endocrine disruption. Hormone concentrations in sediment tended to be greater than concentrations in water. Most BCs were observed at concentrations below those not expected to pose adverse effects to aquatic life. Results indicate that even in remote locations aquatic wildlife can be exposed to pharmaceuticals, hormones, pesticides, and other bioactive contaminants.

Colorado

Biota connect aquatic habitats throughout freshwater ecosystem mosaics

Freshwater ecosystems are linked at various spatial and temporal scales by movements of biota adapted to life in water. We review the literature on movements of aquatic organisms that connect different types of freshwater habitats, focusing on linkages from streams and wetlands to downstream waters. Here, streams, wetlands, rivers, lakes, ponds, and other freshwater habitats are viewed as dynamic freshwater ecosystem mosaics (FEMs) that collectively provide the resources needed to sustain aquatic life. Based on existing evidence, it is clear that biotic linkages throughout FEMs have important consequences for biological integrity and biodiversity. All aquatic organisms move within and among FEM components, but differ in the mode, frequency, distance, and timing of their movements. These movements allow biota to recolonize habitats, avoid inbreeding, escape stressors, locate mates, and acquire resources. Cumulatively, these individual movements connect populations within and among FEMs and contribute to local and regional diversity, resilience to disturbance, and persistence of aquatic species in the face of environmental change. Thus, the biological connections established by movement of biota among streams, wetlands, and downstream waters are critical to the ecological integrity of these systems. Future research will help advance our understanding of the movements that link FEMs and their cumulative effects on downstream waters.

Journal of the American Water Resources Associatio

Thiamine status of rainbow smelt ( Osmerus mordax ) eggs in the Great Lakes, USA

During spring 2006–2009, eggs were collected for analysis of total thiamine from gravid rainbow smelt ( Osmerus mordax ) captured in each of the Great Lakes and two other waters as references for comparison. Mean standard length (mm ± standard error) of gravid females significantly differed between sample waters, with the Atlantic Ocean population being the longest (189 ± 12.3 mm) and Lake Michigan population the shortest (122 ± 0.3 mm). Mean thiamine concentrations (nmol/g ± standard error) for single-year samples for Lake Huron, Lake Michigan, and Little Clear Pond (New York) were 9.9 ± 0.8, 3.9 ± 0.7, and 8.1 ± 2.3 nmol/g, respectively. Thiamine concentrations for multiple-year samples ranged from 1.1 to 15.6 for Lake Ontario, from 2.6 to 3.3 for Lake Erie, from 5.0 to 9.9 for Lake Superior, and from 10.9 to 13.3 for the Atlantic Ocean (Fore River). Although highly variable within populations and across years, thiamine concentrations in most spawning adults appeared to be adequate in all the waters for the years sampled except for 2006 and 2009 in Lake Ontario and 2009 in Lake Erie.

Great Lakes

Remote measurement of canopy water content in giant sequoias (Sequoiadendron giganteum) during drought

California experienced severe drought from 2012 to 2016, and there were visible changes in the forest canopy throughout the State. In 2014, unprecedented foliage dieback was recorded in giant sequoia ( Sequoiadendron giganteum ) trees in Sequoia National Park, in the southern California Sierra Nevada mountains. Although visible changes in sequoia canopies can be recorded, biochemical and physiological responses to drought stress in giant sequoia canopies are not well understood. Ground-based measurements provide insight into the mechanisms of drought responses in trees, but are often limited to few individuals, especially in trees of tall stature such as giant sequoia. Recent studies demonstrate that remotely measured forest canopy water content (CWC) is a general indicator of canopy response to drought, but the underpinning leaf- to canopy-level causes of observed variation in CWC remain poorly understood. We combined field and airborne remote sensing measurements taken in 2015 and 2016 to assess the biophysical responses of giant sequoias to drought. In 49 study trees, CWC was related to leaf water potential, but not to the other foliar traits, suggesting that changes in CWC were made at whole-canopy rather than leaf scales. We found a non-random, spatially varying pattern in mapped CWC, with lower CWC values at lower elevation and along the outer edges of the groves. This pattern was also observed in empirical measurements of foliage dieback from the ground, and in mapped CWC across multiple sequoia groves in this region, supporting the hypothesis that drought stress is expressed in canopy-level changes in giant sequoias. The fact that we can clearly detect a relationship between CWC and foliage dieback, even without taking into account prior variability or new leaf growth, strongly suggests that remotely sensed CWC, and changes in CWC, are a useful measure of water stress in giant sequoia, and valuable for assessing and managing these iconic forests in drought.

California

Identification of Marbon in the Indiana Harbor and Ship Canal

Marbon is isomeric with Dechlorane Plus (DP). Both are produced by the Diels− Alder condensation of hexachlorocyclopentadiene with cyclic dienes, and both have elemental compositions of C18H12Cl12. Dechlorane Plus is commonly found in the environment throughout the world, but Marbon has, so far, only been detected at low levels in one sediment core collected near the mouth of the Niagara River in Lake Ontario. Here we report on the concentrations of Marbon and anti-DP in 59 water samples from five Lake Michigan tributaries [the Grand, Kalamazoo, St. Joseph, and Lower Fox Rivers, and the Indiana Harbor and Ship Canal (IHSC)], 10 surface sediment samples from the IHSC, and 2 surface sediment samples from the Chicago Sanitary and Ship Canal. Three Marbon diastereomers were detected in the water and sediment samples from the IHSC, which is far from the location of its previous detection in Lake Ontario. The sum of the concentrations of the three Marbons was greater in the water from the IHSC (N = 11, median =150 pg/L) compared to those in water from the other four tributaries (N = 11−13, medians =0.9−2.0 pg/L). Marbon concentrations in sediment samples from the IHSC were up to 450 ng/g dry weight. Anti-DP was also measured for comparison. Its concentrations were not significantly different among the water samples, but its sediment concentrations in the IHSC were significantly correlated with those of Marbon. The source of Marbon contamination in the IHSC is not clear.

Indiana

Microplastics in Lake Mead National Recreation Area, USA: Occurrence and biological uptake

Microplastics are an environmental contaminant of growing concern, but there is a lack of information about microplastic distribution, persistence, availability, and biological uptake in freshwater systems. This is especially true for large river systems like the Colorado River that spans multiple states through mostly rural and agricultural land use. This study characterized the quantity and morphology of microplastics in different environmental compartments in two large reservoirs along the Colorado River: Lakes Mead and Mohave, within Lake Mead National Recreation Area. To assess microplastic occurrence, surface water and surficial sediment were sampled at a total of nine locations. Sampling locations targeted different sub-basins with varying levels of anthropogenic impact. Las Vegas Wash, a tributary which delivers treated wastewater to Lake Mead, was also sampled. A sediment core (33 cm long, representing approximately 19 years) was extracted from Las Vegas Bay to assess changes in microplastic deposition over time. Striped bass (Morone saxatilis), common carp (Cyprinus carpio), quagga mussels (Dreissena bugensis), and Asian clams (Corbicula fluminea) were sampled at a subset of locations to assess biological uptake of microplastics. Microplastic concentrations were 0.44-9.7 particles/cubic meter at the water surface and 87.5-1,010 particles/kilogram dry weight (kg dw) at the sediment surface. Sediment core concentrations were 220-2,040 particles/kg dw, with no clear increasing or decreasing trend over time. Shellfish microplastic concentrations ranged from 2.7-105 particles/organism, and fish concentrations ranged from 0-19 particles/organism. Fibers were the most abundant particle type found in all sample types. Although sample numbers are small, microplastic concentrations appear to be higher in areas of greater anthropogenic impact. Results from this study improve our understanding of the occurrence and biological uptake of microplastics in Lake Mead National Recreation Area, and help fill existing knowledge gaps on microplastics in freshwater environments in the southwestern U.S.

Nevada

Effects of acid deposition on ecosystems: Advances in the state of the science

Chapter 2 focused on the environmental results of the ARP, presenting data from national monitoring networks on SO2 and NOx emissions, air quality, atmospheric deposition, surface water chemistry, and visibility. This chapter expands on this information by examining the most recent research into how ecosystems respond to acid deposition, especially the processes that control the recovery of ecosystems as acid deposition decreases. In Chapter 2, two general trends were discussed regarding the current recovery status of affected ecosystems: (1) these ecosystems are trending generally towards recovery, but improvements in ecosystem condition shown by surface water chemistry monitoring data thus far have been less than the improvements in deposition; and (2) ecosystem impacts and trends vary widely by geographic region, but the evidence of improvement is strongest and most evident in the Northeast. These trends are not uniform across the United States, however, and in some regions (e.g., central Appalachian Mountain region), trends in improved water quality are generally not evident. Despite the strong link in many areas between reduced emissions and reduced acidity of atmospheric deposition, the link is less clear between reduced acidity and recovery of the biological communities that live in aquatic and terrestrial ecosystems that have experienced deleterious effects from acid deposition. The recovery of these communities is proceeding at a slower pace than, for example, the improvements in stream and lake ANC would indicate. The goal of this chapter is to synthesize the science in a weightof-evidence manner to provide policy makers with tangible evidence and likely causative factors regarding ecosystem status and recovery patterns to date. This chapter serves as an update to the 2005 NAPAP RTC (NSTC, 2005), with an emphasis on scientific studies and monitoring since 2003, which was the last year for consideration of research results in the 2005 report. Several issues pertinent to ecosystem response to emission controls and acid deposition are receiving increasing attention in the scientific literature and will be discussed in this chapter, including the (1) observed delay in ecosystem recovery in the eastern United States, even with decreases in emissions and deposition over the past 30 years; (2) emerging ecosystem impacts of nitrogen deposition in the western United States; (3) the application of critical deposition loads as a tool for scientists to better inform air quality policies; (4) the role of changes in climate and the carbon cycle as factors that affect the response of ecosystems to acid deposition; and (5) the interaction of multiple pollutants in ecosystems. Throughout this chapter, the value of long-term environmental monitoring data in informing air quality policy will be highlighted, including the limitations of assessing the current status of some ecosystem indicators for which continuous, long-term data are lacking.

Report

Introduction to stream network habitat analysis

Increasing demands on stream resources by a variety of users have resulted in an increased emphasis on studies that evaluate the cumulative effects of basinwide water management programs. Network habitat analysis refers to the evaluation of an entire river basin (or network) by predicting its habitat response to alternative management regimes. The analysis principally focuses on the biological and hydrological components of the riv er basin, which include both micro- and macrohabitat. (The terms micro- and macrohabitat are further defined and discussed later in this document.) Both conceptual and analytic models are frequently used for simplifying and integrating the various components of the basin. The model predictions can be used in developing management recommendations to preserve, restore, or enhance instream fish habitat. A network habitat analysis should begin with a clear and concise statement of the study objectives and a thorough understanding of the institutional setting in which the study results will be applied. This includes the legal, social, and political considerations inherent in any water management setting. The institutional environment may dictate the focus and level of detail required of the study to a far greater extent than the technical considerations. After the study objectives, including species on interest, and institutional setting are collectively defined, the technical aspects should be scoped to determine the spatial and temporal requirements of the analysis. A macro level approach should be taken first to identify critical biological elements and requirements. Next, habitat availability is quantified much as in a "standard" river segment analysis, with the likely incorporation of some macrohabitat components, such as stream temperature. Individual river segments may be aggregated to represent the networkwide habitat response of alternative water management schemes. Things learned about problems caused or opportunities generated may be fed back to the design of new alternatives, which themselves may be similarly tested. One may get as sophisticated an analysis as the decisionmaking process demands. Figure 1 shows a decision point that asks whether the results from the micro- or macrohabitat models display cumulative or synergistic effects. If they do, then network habitat analysis is the appropriate tool. We are left, however, in a difficult bind. We may not know a priori whether the effects are cumulative or synergistic unless some network-type questions are investigated as part of the scoping process. The next several sections raise issues designed to alert the modeler to relevant questions necessary to address this paradox.

Report

Increasing influence of air temperature on upper Colorado River streamflow

This empirical study examines the influence of precipitation, temperature, and antecedent soil moisture on upper Colorado River basin (UCRB) water year streamflow over the past century. While cool season precipitation explains most of the variability in annual flows, temperature appears to be highly influential under certain conditions, with the role of antecedent fall soil moisture less clear. In both wet and dry years, when flow is substantially different than expected given precipitation, these factors can modulate the dominant precipitation influence on streamflow. Different combinations of temperature, precipitation, and soil moisture can result in flow deficits of similar magnitude, but recent droughts have been amplified by warmer temperatures that exacerbate the effects of relatively modest precipitation deficits. Since 1988, a marked increase in the frequency of warm years with lower flows than expected, given precipitation, suggests continued warming temperatures will be an increasingly important influence in reducing future UCRB water supplies.

Geophysical Research Letters

The distribution of bromide in water in the Floridan aquifer system, Duval County, northeastern Florida

Although Duval County, Florida, has ample ground-water resources for public supply, the potential exists for a problem with excessive disinfectant by-products. These disinfectant by-products result from the treatment of raw water containing low concentrations of bromide and naturally occurring organic compounds. Because of this potential problem, the relation of bromide concentrations to aquifer tapped, well location and depth, and chemical characteristics of water in the Floridan aquifer system underlying Duval County were studied to determine if these relations could be applied to delineate water with low-bromide concentrations for future supplies. In 1992, water samples from 106 wells that tap the Floridan aquifer system were analyzed for bromide and major dissolved constituents. A comparison of bromide concentrations from the 1992 sampling with data from earlier studies (1979-80) indicates that higher bromide concentrations were detected during the earlier studies. The difference between the old and new data is probably because of a change in analytical methodology in the analysis of samples. Bromide concentrations exceeded the detection limit (0.10 milligrams per liter) in water from 28 of the 106 wells (26 percent) sampled in 1992. The maximum concentration was 0.56 milligrams per liter. There were no relations between bromide and major dissolved constituents, well depth, or aquifer tapped that would be useful for determining bromide concentrations. Areal patterns of bromide occurrence are not clearly defined, but areas with relatively high bromide concentrations tend to be located in a triangular area near the community of Sunbeam, Florida, and along the St. Johns River throughout Duval County.

Florida

Methods for measuring denitrification: Diverse approaches to a difficult problem

Denitrification, the reduction of the nitrogen (N) oxides, nitrate (NO 3 − ) and nitrite (NO 2 − ), to the gases nitric oxide (NO), nitrous oxide (N 2 O), and dinitrogen (N 2 ), is important to primary production, water quality, and the chemistry and physics of the atmosphere at ecosystem, landscape, regional, and global scales. Unfortunately, this process is very difficult to measure, and existing methods are problematic for different reasons in different places at different times. In this paper, we review the major approaches that have been taken to measure denitrification in terrestrial and aquatic environments and discuss the strengths, weaknesses, and future prospects for the different methods. Methodological approaches covered include (1) acetylene-based methods, (2) 15 N tracers, (3) direct N 2 quantification, (4) N 2 :Ar ratio quantification, (5) mass balance approaches, (6) stoichiometric approaches, (7) methods based on stable isotopes, (8) in situ gradients with atmospheric environmental tracers, and (9) molecular approaches. Our review makes it clear that the prospects for improved quantification of denitrification vary greatly in different environments and at different scales. While current methodology allows for the production of accurate estimates of denitrification at scales relevant to water and air quality and ecosystem fertility questions in some systems (e.g., aquatic sediments, well-defined aquifers), methodology for other systems, especially upland terrestrial areas, still needs development. Comparison of mass balance and stoichiometric approaches that constrain estimates of denitrification at large scales with point measurements (made using multiple methods), in multiple systems, is likely to propel more improvement in denitrification methods over the next few years.

Ecological Applications

Stratigraphy of lower to middle Paleozoic rocks of northern Nevada and the Antler orogeny

Commonly accepted concepts concerning the lower Paleozoic stratigraphy of northern Nevada are based on the assumption that the deep-water aspects of Ordovician to Devonian siliceous strata are due to their origin in a distant oceanic environment, and their presence where we find them is due to tectonic emplacement by the Roberts Mountains thrust. The concept adopted here is based on the assumption that their deep-water aspects are the result of sea-level rise in the Cambrian, and all of the Paleozoic strata in northern Nevada are indigenous to that area. The lower part of the Cambrian consists mainly of shallow-water cross-bedded sands derived from the craton. The upper part of the Cambrian, and part of the Ordovician, consists mainly of deep-water carbonate clastics carried by turbidity currents from the carbonate shelf in eastern Nevada, newly constructed as a result of sea-level rise. Ordovician to mid-Devonian strata are relatively deep-water siliceous deposits, which are the western facies assemblage. The basal contact of this assemblage on autochthonous Cambrian rocks is exposed in three mountain ranges and is clearly depositional in all three. The western facies assemblage can be divided into distinct stratigraphic units of regional extent. Many stratigraphic details can be explained simply by known changes in sea level. Upper Devonian to Mississippian strata are locally and westerly derived orogenic clastic beds deposited disconformably on the western facies assemblage. This disconformity, clearly exposed in 10 mountain ranges, indicates regional uplift and erosion of the western facies assemblage and absence of local deformation. The disconformity represents the Antler orogeny.

Nevada

Eutrophication of freshwater and marine ecosystems

Initial understanding of the links between nutrients and aquatic productivity originated in Europe in the early 1900s, and our knowledge base has expanded greatly during the past 40 yr. This explosion of eutrophication-related research has made it unequivocally clear that a comprehensive strategy to prevent excessive amounts of nitrogen and phosphorus from entering our waterways is needed to protect our lakes, rivers, and coasts from water quality deterioration. However, despite these very significant advances, cultural eutrophication remains one of the foremost problems for protecting our valuable surface water resources. The papers in this special issue provide a valuable cross section and synthesis of our current understanding of both freshwater and marine eutrophication science. They also serve to identify gaps in our knowledge and will help to guide future research.

Limnology and Oceanography

Avoidance behavior of juvenile lake sturgeon (Acipenser fulvescens) exposed to Bayluscide 3.2% Granular Sea Lamprey Larvicide

Avoidance of juvenile lake sturgeons < 100 mm in length in response to application of the Bayluscide 3.2% Granular Sea Lamprey Larvicide was assessed. Clear plexiglas columns (107 cm in height, 30.5 cm in diameter) to evaluate the potential for the normally bottom-dwelling fishes to move vertically in the water column to avoid niclosamide dissolving from the Bayluscide granules. Vertical migration of lake sturgeons to > 15 cm off the bottom of the column was considered avoidance. Lake sturgeons began displaying avoidance behaviors within 4 to 8 min after the granules were applied and continued for up to 60 min. After 60 min, most or all of the sturgeons were near the surface in the treated columns. In contrast, little movement above the 15-cm mark was observed at any time in any of the control columns. The results of this study are similar to a previous study where juvenile lake sturgeons > 100 mm in length showed the ability to avoid granular Bayluscide. Taken together, we conclude that juvenile lake sturgeons of any size range can detect and avoid granular Bayluscide applications.

Report

Assessing the risk of dreissenid mussel invasion in Texas based on lake physical characteristics and potential for downstream dispersal

ebra mussels ( Dreissena polymorpha ) and quagga mussels ( Dreissena bugensis ) were likely introduced from Ponto-Caspian Eurasia to the Laurentian Great Lakes inadvertently via ballast water release in the 1980s and have since spread across the US, including Texas. Their spread into the state, including reservoirs in both Brazos River and Colorado River basins, has resulted in a need to delimit suitable dreissenid habitat and dispersal potential in Texas. The objective of our research was to assess invasion risk in Texas by 1) predicting distribution of suitable habitat of zebra and quagga mussels using Maxent models; 2) refining lake-specific predictions for present zebra mussels via collection of physicochemical data; and 3) assessing the potential for downstream spread of zebra mussels by applying environmental DNA (eDNA) methods in the Leon and Lampasas Rivers downstream from the invaded Lakes Belton and Stillhouse Hollow, respectively. Maxent models did not predict the occurrence of suitable habitat for quagga mussels within Texas. However, our models accurately identified global zebra mussel habitat (AUC = 0.919), and Bioclim layers representing temperature and precipitation data both strongly influenced predictions. Predicted “hotspots” of suitable zebra mussel habitat in Texas occurred along the Red and Sabine Rivers of north and east Texas, as well as patches of suitable habitat in central Texas between the Colorado and Brazos Rivers and extending inland along the Gulf Coast. Most of the Texas panhandle, west Texas extending toward El Paso, and the Rio Grande valley were predicted to provide poor habitat suitability. Collection of physicochemical data (dissolved oxygen, pH, specific conductance, and temperature on-site as well as laboratory analysis for Ca, N, and P) from zebra mussel invaded lakes and a subset of identified high-risk lakes of North and Central Texas, did not aid predictions. Visual inspection of biplots of the first three components of a principle component analysis, which together accounted for ~80% of data variability, did not reveal separation between invaded and uninvaded lakes, and logistic regression analysis also failed to identify predictive relationships between measured variables and invasion status. Using eDNA analysis, we detected the presence of zebra mussel eDNA at 11 of 12 sites and up to at least 90.7 river km downstream from a pair of infested reservoirs. Rate of positive detection among water samples at each site ranged from 1/5 to 5/5, and within positive water samples, rate of detection among technical replicates ranged from 1/8 to 8/8, suggesting considerable heterogeneity in the zebra mussel eDNA signal in both rivers. Furthermore, no clear spatial pattern in detection rate occurred. Thus, a monitoring strategy that combines traditional sampling (e.g. settlement substrate samplers and microscopy) at sites immediately below a dam, and transitioning to more sensitive eDNA analysis at distances further from the dam may represent the most successful strategy for detection of dreissenid mussel downstream dispersal. Overall, we have demonstrated that while quagga mussels do not appear to represent an invasive threat in Texas, suitable habitat for continuing zebra mussel invasion exists within Texas, and stream and river connections may contribute to their spread. The threat of continued expansion of this poster-child for negative invasive species impacts warrants further prevention efforts, management, and research.

continental United States