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At least 541 records · Page 30Linked to original sources

USGS1979

Elimination of endrin by mallard ducks

Endrin is very toxic to birds and has been implicated in the deaths of birds in nature. However, it is not known how rapidly birds eliminate endrin, a factor important in determining how much is accumulated in tissues. In this study, the loss rate of endrin was followed for 64 days in mallard (Anas platyrhynchos) drakes that had been fed 20 ppm endrin for 13 days. The loss from carcass and blood was described by the equation Y = a e b square root of x where Y = the concentration of endrin in ppm, a = the concentration at day 0, e = the base of natural logarithms, b = the first order rate constant for the elimination process, and x = the number of days after cessation of endrin treatment. Endrin was lost rapidly at first; concentrations in carcasses on a wet-weight basis decreased by 50% in the first 3 days. Thereafter, endrin was eliminated more slowly; elimination of 50% of the remainder required 8.9 days, and it took 32.9 days to lose 90% of the original amount.

Toxicology↗

USGS1987

Seasonal variation in diagnostic enzymes and biochemical constituents of captive northern bobwhites and passerines

1. A variety of biochemical measurements were taken periodically in captive northern bobwhite ( Colinus virginianus L.), European starlings ( Sturnus vulgaris L.), red-winged blackbirds ( Agelaius phoeniceus L.) and common grackles ( Quiscalus quiscula L.) to determine whether baseline values remain sufficiently stable throughout the year for general clinical use in the absence of concurrent control specimens. 2. Variables included whole blood hemotacrit and hemoglobin, plasma lactate dehydrogenase, α-hydroxybutyrate dehydrogenase, aspartate aminotransferase, alinine aminotransferase, creatine kinase, butyrylcholinesterase, alkaline phosphatase, glucose, albumin, total protein, creatinine, urea nitrogen, uric acid, cholesterol, and triglycerides, and brain acetylcholinesterase. Butyrl- and acetylcholinesterase were included because of their specific uses in toxicology. 3. Significant seasonal differences were detected for each of the variables except brain acetylcholinesterase in at least one of the species. Significant species differences were detected during at least one season for all of the variables measured. 4. All species were maintained outdoors, but only northern bobwhites came into reproductive condition and showed sex-differences in the clinical variables during their normal breeding season. 5. It was concluded that reference values for the 18 clinical variables measured could be calculated from our data for adult specimens of the species studied, and that results for one species cannot be extrapolated with certainty to any other species. 6. Estimated normal bounds for each of the 18 variables measured by commonly used clinical procedures are presented for reproductively quiescent northern bobwhites, European starlings, red-winged blackbirds, and common grackles.

Comparative Biochemistry and Physiology, Part B: C↗

USGS1980

Effects of petroleum hydrocarbons on hepatic function in the duck

1. The indocyanine green dye clearance test for hepatic function was determined in mallard ducks before and during the chronic ingestion (7 months) of representative paraffinic or aromatic petroleum hydrocarbons (PH). 2. No mortality or visible symptoms of toxicity occurred in any of the tests. Ingestion of 4000 ppm aromatic PH produced significant increases in liver (25%), plasma clearance of indocyanine green (33%) and hepatic blood flow (30%). 3. Although the aromatics elicited a greater hepatic stress response than the paraffins, the ducks tolerated high concentrations of PH for extended periods.

Comparative Biochemistry and Physiology, Part C: C↗

USGS1981

Plasma corticosterone and thyroxine concentrations during chronic ingestion of crude oil in mallard ducks ( Anas platyrhynchos )

1. Blood samples were collected from mallard ducks after 6, 12, and 18 weeks of dietary exposure to mash containing 0.015%, 0.150%, and 1.500% crude oil. 2. Plasma corticosterone concentrations in ducks fed mash containing 0.150% or 1.500% Alaskan Prudhoe Bay crude oil were uniformly depressed when compared to values in untreated control birds. 3. Plasma thyroxine concentration was not altered in ducks chronically exposed to crude oil. 4. The observed alteration in corticosterone concentration could reduce tolerance to temperature and dietary fluctuations in the environment.

Comparative Biochemistry and Physiology, Part C: C↗

USGS2019

Reproductive failure in the Eastern Migratory Population: The interaction of research and management

The reintroduction of the Eastern Migratory Population of Whooping Cranes ( Grus americana ) has shown the most promise of any effort to date toward the establishment of a self-sustaining population. However, reproduction – including both nest success and chick survival – has been a major challenge. Here, we review the research and management efforts deployed to identify and address this challenge. While there is evidence that a proximal cause of nest failure is harassment by blood-feeding black flies ( Simulium spp. ), this is not likely to be the only contributing factor, and we present alternative hypotheses. There is less understanding of the causes of poor chick survival, and we present hypotheses for factors that may be contributing to chick mortality, including environmental, genetic, and behavioral factors. For both nest success and chick survival, we carried out a value of information analysis, using expert judgment, which allowed us to prioritize the alternative hypotheses for evaluation based on the expected management benefit of addressing each hypothesis. On the horizon are multiple opportunities to explore hypotheses about environmental factors as well as genetic and behavioral characteristics of the birds themselves that may contribute to poor reproduction. If bird-specific characteristics, such as genetics or rearing methods, are hindering reproduction, this has important implications for all efforts to restore Whooping Crane populations.

Book chapter↗

USGS2020

Lead speciation, bioaccessibility and source attribution in Missouri's Big River watershed

The Southeast Missouri Lead District is among the most productive lead deposits exploited in modern times. Intensive mining conducted prior to regulations resulted in a legacy of lead contaminated soil, large piles of mine tailings and elevated childhood blood lead levels. This study seeks to identify the source of the lead contamination in the Big River and inform risk to the public. Isotopic analysis indicated the mine tailing piles at the head of the Big River are the primary source of the lead contamination. The isotopic signature of the lead in these mine tailings matched the lead over 100 km downstream. All of the other potential lead sources investigated had different isotopic signatures. Lead concentrations in soils and sediments decrease with distance downstream of the mine tailings piles. Additionally, the speciation of the lead changes from predominantly mineralized forms, such as galena, to adsorbed lead. This is reflected in the in-vitro bioaccessibility assay (IVBA) analysis which shows higher bioaccessibility further downstream, demonstrating the importance of speciation in risk evaluation.

Missouri↗

USGS2005

Disparate effects of constant and annually-cycling daylength and water temperature on reproductive maturation of striped bass (Morone saxatilis)

Adult striped bass ( Morone saxatilis ) were exposed to various combinations of constant or anually-cycling daylength and water temperature. Constant conditions (15 h days, 18 °C) were those normally experienced at spawning and cycling conditions simulated natural changes at Chesapeake Bay latitude. Females exposed to constant long (15 h) days and cycling water temperature (TEMPERATURE group) had blood plasma levels of sex steroids (testosterone [T] and estradiol-17β [E 2 ]) and vitellogenin (Vg), and profiles of oocyte growth, that were nearly identical to those of females held under a natural photothermal cycle (CONTROL group). Several fish from these two groups were induced to spawn fertile eggs. Females constantly exposed to warm water (18 °C), with or without a natural photoperiod cycle (PHOTOPERIOD and STATIC groups, respectively), had diminished circulating levels of gonadal steroid hormones and Vg, impaired deposition of yolk granules in their ooplasm, and decreased oocyte growth, and they underwent premature ovarian atresia. Males exposed to cycling water temperature (CONTROL and TEMPERATURE groups) spermiated synchronously during the natural breeding season, at which time they also had had high plasma androgen (T and 11-ketotestosterone [11-KT]) levels. The timing of spermiation was highly asynchronous among males in groups of fish held constantly at 18 °C (STATIC and PHOTOPERIOD groups) and this asynchrony was associated with diminished plasma androgen levels. Termination of spermiation by males exposed to cycling water temperature coincided with a sharp decline in levels of plasma androgens about a month after water temperature rose above 18 °C. In contrast, most males held constantly at 18 °C sustained intermediate levels of plasma androgens and spermiated until the end of the study in late July. The annual cycle of water temperature clearly plays a prominent role in the initiation, maintenance, and termination of the striped bass reproductive cycle. In females, a decrease in water temperature below values experienced at spawning appears to be required for vitellogenesis and oocyte growth to proceed normally. Constant exposure of males to spawning temperature disrupts synchronous spermiation but also delays testicular regression, which may be useful for spawning fish after the natural reproductive season.

Aquaculture↗

USGS2006

The effect of chronic chromium exposure on the health of Chinook salmon (Oncorhynchus tshawytscha)

This study was designed to determine fish health impairment of Chinook salmon ( Oncorhynchus tshawytscha ) exposed to chromium. Juvenile Chinook salmon were exposed to aqueous chromium concentrations (0–266 μg l −1 ) that have been documented in porewater from bottom sediments and in well waters near salmon spawning areas in the Columbia River in the northwestern United States. After Chinook salmon parr were exposed to 24 and 54 μg Cr l −1 for 105 days, neither growth nor survival of parr was affected. On day 105, concentrations were increased from 24 to 120 μg Cr l −1 and from 54 to 266 μg Cr l −1 until the end of the experiment on day 134. Weight of parr was decreased in the 24/120 μg Cr l −1 treatment, and survival was decreased in the 54/266 μg Cr l −1 treatment. Fish health was significantly impaired in both the 24/120 and 54/266 μg Cr l −1 treatments. The kidney is the target organ during chromium exposures through the water column. The kidneys of fish exposed to the greatest concentrations of chromium had gross and microscopic lesions (e.g. necrosis of cells lining kidney tububules) and products of lipid peroxidation were elevated. These changes were associated with elevated concentrations of chromium in the kidney, and reduced growth and survival. Also, variations in DNA in the blood were associated with pathological changes in the kidney and spleen. These changes suggest that chromium accumulates and enters the lipid peroxidation pathway where fatty acid damage and DNA damage (expressed as chromosome changes) occur to cause cell death and tissue damage. While most of the physiological malfunctions occurred following parr exposures to concentrations ≥120 μg Cr l −1 , nuclear DNA damage followed exposures to 24 μg Cr l −1 , which was the smallest concentration tested. The abnormalities measured during this study are particularly important because they are associated with impaired growth and reduced survival at concentrations ≥120 μg Cr l −1 . Therefore, these changes can be used to investigate the health of resident fish in natural waters with high chromium concentrations as well as provide insight into the mechanisms of chromium toxicity.

Aquatic Toxicology↗

USGS2025

Occurrence and tissue distribution of per- and polyfluoroalkyl substances (PFAS) in fishes from waterbodies with point and non-point sources in Massachusetts, USA

Per- and polyfluoroalkyl substances (PFAS) are persistent environmental contaminants with known bioaccumulative and toxic effects in aquatic ecosystems. This study assessed site-specific differences in PFAS contamination in fish from Ashumet Pond, Sudbury River, and Great Herring Pond (reference site) in Massachusetts. Fish from Ashumet Pond exhibited the highest PFAS concentrations, particularly perfluorooctane sulfonate (PFOS), which exceeded levels in plasma almost 650 times those at the reference site. Principal component analysis identified distinct PFAS profiles at each site, reflecting localized contamination sources. Temporal analysis at Ashumet Pond revealed a substantial increase in plasma PFOS and perfluorodecanoic acid (PFDA) from 2020 to 2022. Tissue distribution analyses showed the highest PFAS concentrations in plasma, followed by liver and muscle, consistent with PFAS binding affinity for blood proteins. Species-specific differences in PFAS bioaccumulation were observed, with largemouth bass ( Micropterus nigricans ) exhibiting higher body burdens than banded killifish ( Diaphanus fundulus ), likely due to trophic position and dietary exposure. Histopathological assessments and gene transcript analyses revealed associations between PFAS exposure and inflammatory responses, oxidative stress, endocrine disruption, and immune-related pathways, with the most pronounced molecular effects observed at the downstream site of the Sudbury River. This study underscores the importance of understanding site-specific contamination sources, exposure pathways, and biological effects of PFAS in fish. These findings would benefit from additional research on sediment contamination, temporal analyses at each site, trophic transfer, and transcriptomic analyses across multiple organs to further elucidate PFAS toxicity mechanisms and guide remediation efforts.

Massachusetts↗

USGS2004

Potential involvement of rainbow trout thrombocytes in immune functions: a study using a panel of monoclonal antibodies and RT-PCR

The functional relationship between fish and mammalian thrombocytes is relatively unknown. In this study, a panel of monoclonal antibodies (mAbs) was used to investigate the functional properties of rainbow trout thrombocytes. The mAbs recognize cell-surface molecules on thrombocytes with molecular weights ranging from 17 to 160 kDa. Flow cytometric and immuno-electron microscopic analyses demonstrate that these molecules are expressed at different levels and that surface expression increased upon activation with bovine collagen. Two of these cell-surface molecules (17 and 21 kDa) were directly involved in collagen-induced aggregation of thrombocytes since aggregation was blocked upon pre-treatment with mAbs that recognize the two surface markers. Interestingly, the percentage of thrombocytes in blood increased after stimulation using different antigens. The transcriptional profile of trout thrombocytes was then examined after immuno-magnetic enrichment using the described mAbs to assess potential roles of trout thrombocytes in immune functions. Trout thrombocytes express components of the MHC class Ia pathway, IL1β, TNFα, TGFβ, the interleukin receptor common γ chain as well as CXC and CC chemokines. MHC class IIB and TNFα were expressed at low levels in resting thrombocytes. No evidence was found for the expression of TCRαβ, Ig heavy chain, CD8α or CK1 mRNA. Taken together, these results suggest that rainbow trout thrombocytes express molecules involved in activation, aggregation and genes encoding proteins, that are involved in antigen presentation and immune regulation.

Developmental and Comparative Immunology↗

USGS2019

In ovo exposure to brominated flame retardants Part II: Assessment of effects of TBBPA-BDBPE and BTBPE on hatching success, morphometric and physiological endpoints in American kestrels

Tetrabromobisphenol A bis(2,3-dibromopropyl ether) (TBBPA-BDBPE) and 1,2-bis(2,4,6-tribromophenoxy)ethane (BTPBE) are both brominated flame retardants (BFRs) that have been detected in birds; however, their potential biological effects are largely unknown. We assessed the effects of embryonic exposure to TBBPA-BDBPE and BTBPE in a model avian predator, the American kestrel ( Falco sparverius ). Fertile eggs from a captive population of kestrels were injected on embryonic day 5 (ED5) with a vehicle control or one of three doses within the range of concentrations that have been detected in biota (nominal concentrations of 0, 10, 50 or 100 ng/g egg; measured concentrations 0, 3.0, 13.7 or 33.5 ng TBBPA-BDBPE/g egg and 0, 5.3, 26.8 or 58.1 ng BTBPE/g egg). Eggs were artificially incubated until hatching (ED28), at which point blood and tissues were collected to measure morphological and physiological endpoints, including organ somatic indices, circulating and glandular thyroid hormone concentrations, thyroid gland histology, hepatic deiodinase activity, and markers of oxidative stress. Neither compound had any effects on embryo survival through 90% of the incubation period or on hatching success, body mass, organ size, or oxidative stress of hatchlings. There was evidence of sex-specific effects in the thyroid system responses to the BTBPE exposures, with type 2 deiodinase (D2) activity decreasing at higher doses in female, but not in male hatchlings, suggesting that females may be more sensitive to BTBPE. However, there were no effects of TBBPA-BDBPE on the thyroid system in kestrels. For the BTPBE study, a subset of high-dose eggs was collected throughout the incubation period to measure changes in BTBPE concentrations. There was no decrease in BTBPE over the incubation period, suggesting that BTBPE is slowly metabolized by kestrel embryos throughout their ∼28-d development. These two compounds, therefore, do not appear to be particularly toxic to embryos of the American kestrel.

Ecotoxicology and Environmental Safety↗

USGS2026

Toxicity of anticoagulant rodenticides on Pacific salmon: Assessing lethal and sublethal effects

To restore native biodiversity on island ecosystems containing invasive rodents, partial- and whole-island eradications generally rely on broadcast baiting with anticoagulant rodenticides (ARs). This approach can result in bait pellets entering aquatic environments, raising concerns about effects to non-target fish. Salmonids are a dominant group of fishes on many temperate islands targeted for rodent eradication, and AR toxicity data for salmonids are limited. Our goal was to determine if coho salmon ( Oncorhynchus kisutch ) are susceptible to coagulopathy and death via exposure to commonly used ARs. We assessed risk of ARs to coho using dose-response curves generated through intraperitoneal injections after determining that coho would not directly ingest the AR baits. Median lethal doses (96-h LD 50 ) estimated using 100 % corn oil carrier were 85.7 µg/g for brodifacoum and 54.0 µg/g for diphacinone. Acetone (30–41 %), used to dissolve ARs in corn oil, reduced the toxicity of diphacinone (LD 50 = 102.3 µg/g, p < 0.001) but not brodifacoum (LD 50 = 73.3 µg/g, p = 0.126) indicating that solvent choice can influence toxicity outcomes. Behavioral changes and onset of mortality differed between the two ARs, with diphacinone acting more rapidly. Tissue analysis supported a difference in toxicokinetics between the two ARs, with significant decreases in liver and muscle residues for diphacinone but not brodifacoum. Sublethal brodifacoum exposure (53.9 µg/g; LD 13 ) impaired blood clotting at 72- and 96- h but returned to baseline by 120 h. No clotting impairment was observed up to 144 h after diphacinone exposure (45.5 µg/g; LD 4 ), suggesting a non-coagulopathy mode of action. These findings will inform risk assessments when considering use of these ARs for rodent management near streams and shorelines and clearly demonstrate that brodifacoum causes coagulopathy in coho.

Ecotoxciology and Environmental Safety↗

USGS2017

Trace element contamination in feather and tissue samples from Anna’s hummingbirds

Trace element contamination (17 elements; Be, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Mo, Cd, Ba, Hg, Tl, and Pb) of live (feather samples only) and deceased (feather and tissue samples) Anna's hummingbirds ( Calypte anna ) was evaluated. Samples were analyzed using inductively coupled plasma-mass spectrometry (ICP-MS; 17 elements) and atomic absorption spectrophotometry (Hg only). Mean plus one standard deviation (SD) was considered the benchmark, and concentrations above the mean + 1 SD were considered elevated above normal. Contour feathers were sampled from live birds of varying age, sex, and California locations. In order to reduce thermal impacts, minimal feathers were taken from live birds, therefore a novel method was developed for preparation of low mass feather samples for ICP-MS analysis. The study found that the novel feather preparation method enabled small mass feather samples to be analyzed for trace elements using ICP-MS. For feather samples from live birds, all trace elements, with the exception of beryllium, had concentrations above the mean + 1 SD. Important risk factors for elevated trace element concentrations in feathers of live birds were age for iron, zinc, and arsenic, and location for iron, manganese, zinc, and selenium. For samples from deceased birds, ICP-MS results from body and tail feathers were correlated for Fe, Zn, and Pb, and feather concentrations were correlated with renal (Fe, Zn, Pb) or hepatic (Hg) tissue concentrations. Results for AA spectrophotometry analyzed samples from deceased birds further supported the ICP-MS findings where a strong correlation between mercury concentrations in feather and tissue (pectoral muscle) samples was found. These study results support that sampling feathers from live free-ranging hummingbirds might be a useful, non-lethal sampling method for evaluating trace element exposure and provides a sampling alternative since their small body size limits traditional sampling of blood and tissues. The results from this study provide a benchmark for the distribution of trace element concentrations in feather and tissue samples from hummingbirds and suggests a reference mark for exceeding normal. Lastly, pollinating avian species are minimally represented in the literature as bioindicators for environmental trace element contamination. Given that trace elements can move through food chains by a variety of routes, our study indicates that hummingbirds are possible bioindicators of environmental trace element contamination.

Ecological Indicators↗

USGS2021

Assessment of sea lamprey (Petromyzon marinus) diet using DNA metabarcoding of feces

Sea lamprey ( Petromyzon marinus ) are invasive in the Laurentian Great Lakes, parasitize large-bodied fishes, and therefore are the focus of an international control program. However, damage caused by sea lamprey to modern day fish stocks remains uncertain because diet analysis of juvenile sea lamprey has been challenging; they feed on blood and are difficult to randomly sample in the lakes. Here, both challenges were addressed by showing that DNA metabarcoding of fecal material can be used to identify the diet of actively feeding juvenile sea lamprey, and can also be used to determine what non-feeding adult sea lamprey captured in streams fed on while parasitizing fish. Fecal samples from juvenile sea lamprey that were feeding on lake trout in northern Lake Huron overwhelmingly contained lake trout ( Salvelinus namaycush ) DNA (90%), while smaller percentages contained lake whitefish ( Coregonus clupeaformis ; 5%) and longnose sucker ( Catostomus catostomus ; 5%) DNA. Fecal samples from adult sea lamprey captured from a tributary to northern Lake Huron overwhelmingly contained longnose and white sucker DNA ( Catostomus spp.; 80%), while a smaller percentage contained lake trout DNA (10%). Diet composition of adult sea lamprey sampled in the tributary (Black Mallard Creek) was more diverse than juvenile diet composition. DNA metabarcoding suggests that Catostomus spp. may be an important host fish in northern Lake Huron for sea lamprey prior to spawning. Future research could investigate how diet varies across years and lakes and the prevalence and sources of DNA contamination. Application of DNA metabarcoding for diet assessment may be practical for identifying populations of invasive sea lamprey that feed on highly valued fishes and help guide restoration of lampreys worldwide.

Michigan↗

USGS2021

Establishment of baseline cytology metrics in nestling American kestrels (Falco sparverius): Immunomodulatory effects of the flame retardant isopropylated triarylphosphate isomers

Avian populations must mount effective immune responses upon exposure to environmental stressors such as avian influenza and xenobiotics. Although multiple immune assays have been tested and applied to various avian species, antibody-mediated immune responses in non-model avian species are not commonly reported due to the lack of commercially available species-specific antibodies. The objectives of the present study were to advance methods for studying wild bird immune responses and to apply these to the evaluation of cytological responses after exposure of American kestrels, Falco sparverius, to a commercial flame retardant mixture containing isopropylated triarylphosphate isomers (ITP). Hatchlings were gavaged daily with safflower oil or 1.5 ug/g bw/day of ITP suspended in safflower oil, then bled on days 9, 17, and 21. The ITP treatment group ( n = 18) and a subset of controls (Poly I:C treatment group; n = 10) were injected on days 9 and 15 with a synthetic analog of viral double-stranded RNA, polyinosinic:polycytidylic acid (Poly I:C), a toll-like receptor ligand and synthetic viral mimic, and responses compared to a sham injected control group (n = 8). The hypotheses tested whether kestrels showed immunological differences among treatment groups, genetic sex, and/or white blood cell (WBC) subpopulation type over time. A flow cytometry (FCM) gating strategy categorized heterophils (H), lymphocytes (L), and monocytes (M) and their proportions, and measured relative fluorescence in response to anti-chicken CD4 binding. Fluorescent cell surfaces and some granular/vacuolar inclusions were visualized by epifluorescence microscopy. A fourth subpopulation with higher levels of granularity than M but less than H became increasingly apparent with time and was gated along with the H subpopulation; its frequency of occurrence was lowest in the ITP group ( P = 0.0023). The percentages of cells differed among treatment groups, days, and sexes ( P = 0.0001). For both sexes, percentages of H and L were higher than M in control and Poly I:C. In the ITP group, L percentages were higher than H and M ( P = 0.0457), and H and L were higher than M on days 9 and 21 ( P = 0.0001). The ratios of H:L and H:WBC, indicators of robust immunity, were also higher on days 9 and 21 than on 17 ( P = 0.0079). For each sex, the highest levels of activity measured by FCM geometric means (GEO) of fluorescence (indicative of antibody binding) were observed on day 9 ( P = 0.0001 female, and P = 0.0011 male) in H over both L and M ( P < 0.0001 for each). In males, GEO of the Poly I:C group was higher than that of the ITP group ( P = 0.0374), with no difference observed among females over all days. By using a FCM algorithm for population comparisons of fluorescence to investigate binding within H, the T(x) scores indicated higher fluorescence in control and Poly I:C groups over ITP ( P = 0.0001). Unlike chickens, Gallus gallus , which express CD4 primarily on L, kestrels bound the commercial antibody primarily within the gated H subpopulation, suggesting an immunophenotypic difference between taxa, despite a ~60% identity of Falco CD4 amino acid sequences with chicken CD4. The emergent cell subset within the gated H presented dendritic-like cell (DLC) morphological and functional properties, apparently serving as an effector cell. This study adds interpretive context to ecological investigations of infection and of potential immunomodulation by emerging compounds, whereby the early innate responses are mediated by the various cell subsets serving as useful quantitative markers of immunological condition. Data showed that dietary exposure to ITP was immunosuppressive for male and female kestrels over the course of the experiment, reducing DLC frequency compared to the Poly I:C controls. Heterophils and DLC were important in facilitating innate immunological responses.

Environment International↗

USGS2018

Respiratory hazard assessment of combined exposure to complete gasoline exhaust and respirable volcanic ash in a multicellular human lung model at the air-liquid interface

Communities resident in urban areas located near active volcanoes can experience volcanic ash exposures during, and following, an eruption, in addition to sustained exposures to high concentrations of anthropogenic air pollutants ( e.g., vehicle exhaust emissions). Inhalation of anthropogenic pollution is known to cause the onset of, or exacerbate, respiratory and cardiovascular diseases. It is further postulated similar exposure to volcanic ash can also affect such disease states. Understanding of the impact of combined exposure of volcanic ash and anthropogenic pollution to human health, however, remains limited. The aim of this study was to assess the biological impact of combined exposure to respirable volcanic ash (from Soufrière Hills volcano (SHV), Montserrat and Chaitén volcano (ChV), Chile; representing different magmatic compositions and eruption styles) and freshly-generated complete exhaust from a gasoline vehicle. A multicellular human lung model (an epithelial cell-layer composed of A549 alveolar type II-like cells complemented with human blood monocyte-derived macrophages and dendritic cells cultured at the air-liquid interface) was exposed to diluted exhaust (1:10) continuously for 6 h, followed by immediate exposure to the ash as a dry powder (0.54 ± 0.19 μg/cm 2 and 0.39 ± 0.09 μg/cm 2 for SHV and ChV ash, respectively). After an 18 h incubation, cells were exposed again for 6 h to diluted exhaust, and a final 18 h incubation (at 37 °C and 5% CO 2 ). Cell cultures were then assessed for cytotoxic, oxidative stress and (pro-)inflammatory responses. Results indicate that, at all tested (sub-lethal) concentrations, co-exposures with both ash samples induced no significant expression of genes associated with oxidative stress ( HMOX1, NQO1 ) or production of (pro-)inflammatory markers (IL-1β, IL-8, TNF-α) at the gene and protein levels. In summary, considering the employed experimental conditions, combined exposure of volcanic ash and gasoline vehicle exhaust has a limited short-term biological impact to an advanced lung cell in vitro model.

Environmental Pollution↗

USGS2020

Arsenic-related oxidative stress in experimentally dosed wild great tit nestlings

Arsenic (As) is broadly distributed due to natural and anthropogenic sources, and it may cause adverse effects in birds. However, research on other elements (Pb, Hg and Cd) has been prioritized, resulting in scarce data on As exposure and related effects in wild birds. One of the mechanisms responsible for As toxicity is oxidative stress. Therefore, the aim of this study was to investigate if environmentally relevant As levels affected oxidative stress biomarkers in great tits ( Parus major ). This is the first field experiment studying the effects of As on oxidative stress in wild passerines. Wild great tit nestlings were orally dosed with sodium arsenite (Control: water, Low dose: 0.2 μg g −1 d −1 and High dose: 1 μg g −1 d −1 ; from day 3 to day 13 post-hatching). We intended to reach As concentrations similar to those at which passerines are exposed to at actual polluted areas. We compared the responses to the experimental manipulations (High, Low and Control groups) with those in an As/metal-exposed population breeding close to a Cu–Ni smelter in Finland (Smelter group). A set of antioxidants (tGSH, GSH:GSSG ratio, CAT, SOD, GST and GPx), and oxidative damage biomarkers (lipid peroxidation, protein carbonylation, 8-hydroxy-2′-deoxyguanosine formation in DNA, and telomere length) were explored in blood. Arsenic administration had no significant effect on most of the biomarkers measured: only the CAT activity was lower in the High As group and the GPx activity was enhanced in the Smelter group compared to the Control. Our results suggest that the dose and duration of the As exposure was not enough to induce oxidative damage in red cells of great tit nestlings. In spite of this, nestlings dosed with 1 μg g −1 d −1 of sodium arsenite showed non-significantly higher oxidative stress biomarkers than controls, suggesting that we were close to an effect level for the redox-defense system. Oxidative effects at equivalent As levels combined with other stressors cannot be dismissed.

Harjavalta↗

USGS2026

Per- and polyfluoroalkyl substances and pesticides in black bears (Ursus americanus) and grizzly bears (Ursus arctos horribilis) from Montana's Cabinet-Yaak ecosystem: A baseline assessment of emerging and legacy contaminants

Per- and polyfluoroalkyl substances (PFAS) and pesticides are globally distributed contaminants that persist in terrestrial food webs, yet baseline data for large omnivores in the continental U.S. remain limited. This study quantified PFAS and pesticides in whole blood from free-ranging black bears ( Ursus americanus , n = 15) and grizzly bears ( Ursus arctos horribilis , n = 9) in the Cabinet-Yaak Ecosystem of northwestern Montana. Using high-resolution mass spectrometry-based methods (GC-MS/MS and LC-MS/MS), 52 PFAS and 186 pesticides were analyzed. PFAS were detected in all bears, with total PFAS concentrations ranging from 79.5 to 317 pg/mL. PFAS profiles were dominated by long-chain perfluoroalkyl carboxylic acids, particularly PFOA, PFDA, and PFUnDA, with minimal contribution from short-chain compounds. Species was the strongest ecological predictor of PFAS concentrations, although variability across age, sex, and capture location were observed. Only two pesticides, piperonyl butoxide and permethrin, were detected at quantifiable concentrations (2070–12,600 pg/mL), and no correlations were observed between pesticide and PFAS concentrations, indicating independent exposure pathways. The predominance of long-chain PFAS suggests diffuse environmental sources and bioaccumulation within terrestrial food webs. Although measured concentrations were generally low relative to other wildlife toxicological studies, the persistence and protein-binding properties of long-chain PFAS warrant continued monitoring, particularly for long-lived omnivores. These results establish baseline contaminant concentrations for two apex omnivores in a remote ecosystem and highlight the utility of large mammals as integrative sentinels of environmental contaminant exposure for regional monitoring and wildlife conservation. Continued monitoring in the Cabinet-Yaak and across western North America will be critical for assessing temporal trends, identifying new contaminant sources, and evaluating ecological health in bear populations.

Montana↗