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Water and Streambed Sediment Quality, and Ecotoxicology of a Stream along the Blue Ridge Parkway, Adjacent to a Closed Landfill, near Roanoke, Virginia: 1999

A study was done of the effects of a closed landfill on the quality of water and streambed sediment and the benthic macroinvertebrate community of an unnamed stream and its tributary that flow through Blue Ridge Parkway lands in west-central Virginia. The primary water source for the tributary is a 4-inch polyvinyl chloride (PVC) pipe that protrudes from the slope at the base of the embankment bordering the landfill. An unusual expanse of precipitate was observed in the stream near the PVC pipe. Stream discharge was measured and water and streambed sediment samples were collected at a nearby reference site and at three sites downstream of the landfill in April and September 1999. Water samples were analyzed for major ions, nitrate, total and dissolved metals, total dissolved solids, total organic carbon, and volatile and semivolatile organic compounds, including organochlorine pesticides and polychlorinated biphenyls (PCBs). Streambed sediment samples were analyzed for total metals, total organic carbon, percent moisture, and volatile and semivolatile organic compounds, including organochlorine pesticides and PCBs. The benthic macroinvertebrate community within the stream channel also was sampled at the four chemical sampling sites and at one additional site in April and September. Each of the five sites was assessed for physical habitat quality. Water collected periodically at the PVC pipe discharge between November 1998 and November 1999 was used to conduct 48-hour acute and 7-day chronic toxicity tests using selected laboratory test organisms. Two 10-day chronic toxicity tests of streambed sediments collected near the discharge pipe also were conducted. Analyses showed that organic and inorganic constituents in water from beneath the landfill were discharged into the sampled tributary. In April, 79 percent of inorganic constituents detected in water had their highest concentrations at the site closest to the landfill; at the same site, 59 percent of inorganic constituents detected in streambed sediments were at their lowest concentration. The low dissolved-oxygen concentration and relatively low pH in ground water from beneath the landfill probably had a direct effect on the solubility of metals and other constituents, resulting in the high concentration of inorganic constituents in water, low concentration in sediment, and the development of the precipitate. Most constituents in water in April were progressively lower in concentration from the landfill site downstream. The highest concentrations for 59 percent of constituents detected in sediment were at the farthest downstream site, suggesting that the inorganic constituents came out of solution as the stream water was exposed to the atmosphere. In September, 52 percent of inorganic constituents detected in water were at their highest concentrations at the site nearest the landfill. Of inorganic constituents detected in streambed sediments in September, 60 percent were at their highest concentrations near the landfill. A storm that occurred a few days prior to the September sampling probably affected the preceding steady-state conditions and the distribution of constituents in sediment along the stream. Concentrations of many inorganic constituents in water remained elevated at the farthest downstream site in comparison to the reference site in April and September, indicating that concentrations did not return to background concentrations. In April and September, most of the 17 organic compounds detected in water, including volatile organic and semivolatile organic compounds, were collected in samples near the landfill, and most concentrations were below their respective reporting limits. Probably because of their volatility, few organic compounds were detected at sites downstream of that site. A total of 17 discrete organic compounds were detected in sediment samples in either April or September, including trichloroethene and tetrachloroethene along with their degrad

Water-Resources Investigations Report↗

Ecotoxicological damage from zinc smelting at Palmerton, Pennsylvania

The two zinc smelters at Palmerton, PA emitted huge amounts of contaminants ( 260,000 t of Zn, 3,300 t of Cd, 6,800 t of Pb) to the air and severely damaged the forest on Blue Mountain. The high Zn concentrations in soil killed tree seedlings by inhibiting root elongation. The result was a forest with too few young trees. When natural stresses such as fire challenged the forest, the forest failed to regenerate, and the exposed soil eroded down the steep slopes. Tree species that could sprout were favored over those that developed from seeds. As a result of high zinc concentrations, the lichen and moss communities were depauperate for at least 20 km along Blue Mountain. The denuded areas are in the process of being reclaimed with the addition of a mixture of fly ash and sewage sludge, which is seeded with grasses tolerant to the harsh conditions. In preliminary experiments, the fly ash and sewage sludge mixture was stable, despite the steepness of the slopes of the reclaimed sites on Blue Mountain. Zinc emissions reduced the decomposition rate of plant material on Blue Mountain. The partially decomposed litter, in particular, accumulated on the surface of the mineral soil. The populations of both microorganisms and arthropods were greatly reduced in soils near the smelters. Samples of litter collected from sites spanning 30 km were toxic to woodlice, and Zn was shown to be the toxic factor. A white-tailed deer examined had a very high renal Zn concentration and an articular lesion in one of its hind legs that closely resembled the lesions reported in Zn-poisoned horses. Zinc concentrations were regulated in wildlife tissues and were not reliable indicators of exposure, except in extreme cases. Two songbirds, a shrew, and several rabbits contained Pb concentrations that were suggested to be toxic. Shrews and ground-feeding songbirds accumulated relatively high concentrations of Pb. Exposure to Pb seemed to be related to the amount of soil that an animal ingests. Some white-tailed deer and cottontails had renal Cd concentrations near 600 ppm w.w., which is associated with renal damage in other mammals. The accumulation of Cd was greatest in wildlife that lived longest. The destruction of the habitat for wildlife on Blue Mountain decreased wildlife populations. Populations of insectivorous birds, however, were depressed well beyond the area of obvious damage to the forest. Forest-floor salamanders were completely absent from study sites spanning 18 km along Blue Mountain. Actual reductions in wildlife populations were much greater than would have been predicted from habitat suitability models alone.

Book chapter↗

Ecotoxicology

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Book chapter↗

The Chernobyl Nuclear Power Plant accident: ecotoxicological update

The accident at the Chernobyl, Ukraine, nuclear reactor on 26 April 1986 released large amounts of radiocesium and other radionuclides into the environment, contaminating much of the northern hemisphere, especially Europe. In the vicinity of Chernobyl, at least 30 people died, more than 115,000 others were evacuated, and consumption of milk and other foods was banned because of radiocontamination. At least 14,000 human cancer deaths are expected in Russia, Belarus, and the Ukraine as a direct result of Chernobyl. The most sensitive local ecosystems, as judged by survival, were the soil fauna, pine forest communities, and certain populations of rodents. Elsewhere, fallout from Chernobyl significantly contaminated freshwater and terrestrial ecosystems and flesh and milk of domestic livestock; in many cases, radionuclide concentrations in biological samples exceeded current radiation protection guidelines. Reindeer (Rangifer tarandus) in Scandinavia were among the most seriously afflicted by Chernobyl fallout, probably because their main food during winter (lichens) is an efficient absorber of airborne particles containing radiocesium. Some reindeer calves contaminated with 137Cs from Chernobyl showed 137Cs-dependent decreases in survival and increases in frequency of chromosomal aberrations. Although radiation levels in the biosphere are declining with time, latent effects of initial exposure--including an increased frequency of thyroid and other cancers--are now measurable. The full effect of the Chernobyl nuclear reactor accident on natural resources will probably not be known for at least several decades because of gaps in data on long-term genetic and reproductive effects and on radiocesium cycling and toxicokinetics.

Book chapter↗

Ecotoxicology: Lead

Lead (Pb) is a naturally occurring metallic element; trace concentrations are found in all environmental media and in all living things. However, certain human activities, especially base metal mining and smelting; combustion of leaded gasoline; the use of Pb in hunting, target shooting, and recreational angling; the use of Pb-based paints; and the uncontrolled disposal of Pb-containing products such as old vehicle batteries and electronic devices have resulted in increased environmental levels of Pb, and have created risks for Pb exposure and toxicity in invertebrates, fish, and wildlife in some ecosystems.

Book chapter↗

Contaminant exposure and potential effects on terrestrial vertebrates residing in the National Capital Region network and Mid-Atlantic network

Part of the mission of the National Park Service is to preserve the natural resources, processes, systems, and associated values of its units in an unimpaired condition. Environmental contamination and pollution processes are well recognized stressors addressed by its management policies and plans. A recent study indicates that contemporary terrestrial vertebrate ecotoxicological data are lacking for 59 of 126 Park Service units located in coastal watersheds exhibiting serious water quality problems or high vulnerability to pollution. Based upon these findings, a more in-depth evaluation of contaminant threats and ecotoxicological data gaps related to terrestrial vertebrates was undertaken at 23 Inventory and Monitoring National Park units in National Capital Region and Mid-Atlantic Networks. Ecotoxicological data were compiled for each park unit through literature searches and meetings with Park Service personnel. Information on contemporary and on persistent legacy pollutants in air, water, soil, and terrestrial vertebrates (amphibians, reptiles, birds and mammals) were evaluated. To identify contaminant threats in proximity to the 23 Park Service study units, data was gathered on National Priority List Superfund sites, Section 303(d) Impaired Waterbodies, the number and relative toxicity of current use pesticides and herbicides, Toxic Release Inventory sites and discharge of priority pollutants, and Fish Consumption Advisories. A metric was derived that described the quality and quantity of existing data for each park, and in combination with known contaminant threats, park units in need of additional study were identified. Results demonstrated that over half of the Park Service study units are near Toxic Release Inventory sites discharging dioxins, polychlorinated biphenyls, lead or mercury into air or water, and fish consumption advisories are in effect at or near 22 of the 23 study units. Pesticide and herbicide use at the park units is minimal, with the exception of those units with significant agricultural leases. Despite highly regulated use, many of the pesticides and herbicides applied are believed to be highly toxic to amphibians, and some of the compounds are also highly toxic to birds. Only 70 reports were found that describe terrestrial vertebrate ecotoxicology data on or near the study units. Of the greater than 75,000 compounds in commerce in the United States, existing terrestrial vertebrate exposure and effects data in the pr esent study were limited to 58 legacy organochlorine pesticides, polychlorinated biphenyls and individual congeners, insecticides and rodenticides, metals, and some contemporary compounds (e.g., polybrominated diphenyl ether flame retardants, and alkylphenol and ethoxylate surfactants). Based upon these and other findings, ecotoxicological monitoring and research investigations of terrestrial vertebrates are warranted at several National Parks. These include Shenandoah National Park, Richmond National Battlefield, Chesapeake & Ohio Canal National Historic Park, Valley Forge National Historic Park, Hopewell Furnace National Historic Site, Monocacy National Battlefield, and Harpers Ferry National Historic Park. The types of investigations vary according to the species present at these parks and potential contaminant threats, but should focus on contemporary use pesticides and herbicides, polychlorinated biphenyls, mercury, lead, and perhaps, emerging contaminants including antibiotics, flame retardants, pharmaceuticals, and surfactants. Other management recommendations include additional training for natural resource staff members in the area of ecotoxicology, inclusion of terrestrial vertebrate contaminant monitoring and the Contaminant Assessment Process (U.S. Geological Survey Biomonitoring of Environmental Status and Trends Project) into the National Park Service Vital Signs Program, development of protocols for hand ling and toxicological analysis of dead or seemingly affected wildlife, consideration of some alternative methods and compounds for pest management and weed control, and use of non-toxic fishing tackle by visitors.

Maryland, Pennsylvania, Virginia↗

A framework for prioritizing contaminants in retrospective ecological assessments: Application in the Milwaukee Estuary (Milwaukee, WI)

Watersheds are subjected to diverse anthropogenic inputs, exposing aquatic biota to a wide range of chemicals. Detection of multiple, different chemicals can challenge natural resource managers who often have to determine where to allocate potentially limited resources. Here, we describe a weight-of-evidence framework for retrospectively prioritizing aquatic contaminants. To demonstrate framework utility, we used data from 96-h caged fish studies to prioritize chemicals detected in the Milwaukee Estuary (WI, USA; 2017–2018). Across study years, 77/178 targeted chemicals were detected. Chemicals were assigned prioritization scores based on spatial and temporal detection frequency, environmental distribution, environmental fate, ecotoxicological potential, and effect prediction. Chemicals were sorted into priority bins based on the intersection of prioritization score and data availability. Data-limited chemicals represented those that did not have sufficient data to adequately evaluate ecotoxicological potential or environmental fate. Seven compounds (fluoranthene, benzo[ a ]pyrene, pyrene, atrazine, metolachlor, phenanthrene, and DEET) were identified as high or medium priority and data sufficient and flagged as candidates for further effects-based monitoring studies. Twenty-one compounds were identified as high or medium priority and data limited and flagged as candidates for further ecotoxicological research. Fifteen chemicals were flagged as the lowest priority in the watershed. One of these chemicals (2-methylnaphthalene) displayed no data limitations and was flagged as a definitively low-priority chemical. The remaining chemicals displayed some data limitations and were considered lower-priority compounds (contingent on further ecotoxicological and environmental fate assessments). The remaining 34 compounds were flagged as low or medium priority. Altogether, this prioritization provided a screening-level (non-definitive) assessment that could be used to focus further resource management and risk assessment activities in the Milwaukee Estuary. Furthermore, by providing detailed methodology and a practical example with real experimental data, we demonstrated that the proposed framework represents a transparent and adaptable approach for prioritizing contaminants in freshwater environments. Integr Environ Assess Manag 2023;00:1–21. © 2022 SETAC

Wisconsin↗

EthoCRED: A framework to guide reporting and evaluation of the relevance and reliability of behavioural ecotoxicity studies

Behavioural analysis has been attracting significant attention as a broad indicator of sub-lethal toxicity and has secured a place as an important subdiscipline in ecotoxicology. Among the most notable characteristics of behavioural research, compared to other established approaches in sub-lethal ecotoxicology (e.g. reproductive and developmental bioassays), are the wide range of study designs being used and the diversity of endpoints considered. At the same time, environmental hazard and risk assessment, which underpins regulatory decisions to protect the environment from potentially harmful chemicals, often recommends that ecotoxicological data be produced following accepted and validated test guidelines. These guidelines typically do not address behavioural changes, meaning that these, often sensitive, effects are not represented in hazard and risk assessments. Here, we propose a new tool, the EthoCRED evaluation method, for assessing the relevance and reliability of behavioural ecotoxicity data, which considers the unique requirements and challenges encountered in this field. This method and accompanying reporting recommendations are designed to serve as an extension of the “Criteria for Reporting and Evaluating Ecotoxicity Data (CRED)” project. As such, EthoCRED can both accommodate the wide array of experimental design approaches seen in behavioural ecotoxicology, and could be readily implemented into regulatory frameworks as deemed appropriate by policy makers of different jurisdictions to allow better integration of knowledge gained from behavioural testing into environmental protection. Furthermore, through our reporting recommendations, we aim to improve the reporting of behavioural studies in the peer-reviewed literature, and thereby increase their usefulness to inform chemical regulation.

Biological Reviews↗

Effects of current-use pesticides on amphibians

For many years, amphibians were understudied in the ecotoxicological literature. In 1989, the Canadian Wildlife Service published a comprehensive review of studies examining the effects of contaminants on amphibians (Power et al. 1989). Just 10 years later, the same organization published an updated review that included twice the number of studies (Pauli et al. 2000), indicating rapid growth in the field of amphibian ecotoxicology. However, Sparling et al. (2000) point out that the number of amphibian ecotoxicological studies remains modest relative to research utilizing other taxa. Relyea and Hoverman (2006) also report that amphibian data appear to be lagging behind other taxa, despite an increasing number of ecotoxicological studies involving freshwater ecosystems in general.

Book chapter↗

Silver nanoparticles: Behaviour and effects in the aquatic environment

This review summarises and evaluates the present knowledge on the behaviour, the biological effects and the routes of uptake of silver nanoparticles (Ag NPs) to organisms, with considerations on the nanoparticle physicochemistry in the ecotoxicity testing systems used. Different types of Ag NP syntheses, characterisation techniques and predicted current and future concentrations in the environment are also outlined. Rapid progress in this area has been made over the last few years, but there is still a critical lack of understanding of the need for characterisation and synthesis in environmental and ecotoxicological studies. Concentration and form of nanomaterials in the environment are difficult to quantify and methodological progress is needed, although sophisticated exposure models show that predicted environmental concentrations (PECs) for Ag NPs in different environmental compartments are at the range of ng L − 1 to mg kg − 1 . The ecotoxicological literature shows that concentrations of Ag NPs below the current and future PECs, as low as just a few ng L − 1 , can affect prokaryotes, invertebrates and fish indicating a significant potential, though poorly characterised, risk to the environment. Mechanisms of toxicity are still poorly understood although it seems clear that in some cases nanoscale specific properties may cause biouptake and toxicity over and above that caused by the dissolved Ag ion. This review concludes with a set of recommendations for the advancement of understanding of the role of nanoscale silver in environmental and ecotoxicological research.

Environment International↗

Derivation and characterization of environmental hazard concentrations for chemical prioritization: A case study in the Great Lakes tributaries

Ongoing anthropogenic activities and analytical advancements yield continuously expanding lists of environmental contaminants. This represents a challenge to environmental managers, who must prioritize chemicals for management actions (e.g., restriction, regulation, remediation) but are often hindered by resource limitations. To help facilitate prioritization efforts, this study presents several strategies for deriving environmental hazard concentrations using publicly accessible data and open-source computational tools. Using a Great Lakes tributaries aquatic monitoring dataset as a case study, environmental hazard concentrations were obtained or derived for 334 organic chemicals. These concentrations were based on (1) current water quality guidelines; (2) apical screening values; (3) apical and (4) nonapical effect concentrations from the ECOTOXicology Knowledgebase; (5) in vitro effect concentrations from the ToxCast database; (6) cytotoxic burst concentrations collated from the Comptox Dashboard; (7) “estimated screening values” derived from modeled or estimated data and available from various regulatory and nonregulatory agencies; (8) pharmaceutical potency estimates from the MaPPFAST database; and (9) quantitative structure-activity relationship (QSAR)–derived acute toxicity estimates. Environmental fate data included aquatic half-lives and bioconcentration factors collated from the Comptox Dashboard or estimated using QSARs. To identify patterns that could be used for characterization, availability of ecotoxicological concentrations and environmental fate data were evaluated. Furthermore, exceedances of hazard concentrations were evaluated and compared across diverse ecotoxicological data types. Altogether, by providing detailed methodology and practical examples generated with real monitoring data, this study demonstrated that these hazard concentration derivation strategies can be efficiently and effectively used with large, complex datasets and identified critical considerations for future prioritization efforts.

Great Lakes region↗

Environmental contaminant exposure data and monitoring priorities for wild terrestrial vertebrates at national parks in coastal and estuarine habitat

The Biomonitoring of Environmental Status and Trends (BEST) Project of the U.S. Geological Survey (USGS) assesses the exposure and effects of environmental contaminants on select species and habitats in the United States. One of the many BEST Project activities entails the development of decision-support tools to assist in the identification of chemical threats to species and lands under the stewardship of the Department of the Interior. Although there are many ecotoxicological monitoring programs that focus on aquatic species and habitats, there are currently no large-scale efforts that are focused on terrestrial vertebrates in the United States. Nonetheless, organochlorine contaminants, metals, and new pollutants continue to pose hazards to terrestrial vertebrates at many spatial scales (ranging from small hazardous-waste-site point sources to entire watersheds). To evaluate and prioritize pollutant hazards for terrestrial vertebrates, a ?Contaminant Exposure and EffectsTerrestrial Vertebrates? (CEE-TV) database (www.pwrc.usgs.gov/contaminants-online) was developed. The CEE-TV database has been used to conduct simple searches for exposure and biological effects information for a given species or location, identification of temporal contaminant exposure trends, information gap analyses for national wildlife refuge and national park units, and ranking of terrestrial vertebrate ecotoxicological information needs based on data density and water quality problems. Despite widespread concerns about environmental contamination, during the past decade only about one-half of the coastal National Park units appear to have terrestrial vertebrate ecotoxicological data. Based upon known environmental contaminant hazards, it is recommended that regionalized monitoring programs or efforts focused on lands managed by the Department of the Interior should be undertaken to prevent serious natural resource problems.

Book chapter↗

Are behavioural ecotoxicity endpoints relevant at the population level? Evidence-based insights for environmental protection

A substantial body of evidence exists demonstrating that exposure to environmental contaminants can alter animal behavior. Moreover, methodological and technological advancements, as well as increasing standardization, mean that behavioral ecotoxicity studies are more rigorous and reliable than ever before. Despite this, behavioral data are still seldom used in the risk assessment and regulation of chemicals. This is partly due to a lack of clarity among some stakeholders about whether changes in behavior at the individual level result in population-level outcomes. To address this, we first consider the state of evidence within the field of behavioral ecotoxicology linking individual-level behavioral alterations with population-level consequences. We then assess the evidence from behavioral ecology and other neighboring fields that supports this link. Further, we evaluate whether some behavioral endpoints are more easily tied to population-level changes than others. In this regard, we propose combining insights from two complementary ecological frameworks─the functional trait framework and the limiting traits framework─to evaluate which behaviors should be prioritized in ecotoxicological research and regulatory efforts. We contend that the link between behavioral changes and population-level outcomes is evident, with behavioral endpoints representing a highly valuable yet so far underutilized line of evidence in applied environmental protection.

Environmental Science and Technology↗