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HESS opinions: Beyond the long-term water balance: Evolving Budyko's supply–demand framework for the Anthropocene towards a global synthesis of land-surface fluxes under natural and human-altered watersheds

Global hydroclimatic conditions have been substantially altered over the past century by anthropogenic influences that arise from the warming global climate and from local/regional anthropogenic disturbances. Traditionally, studies have used coupling of multiple models to understand how land-surface water fluxes vary due to changes in global climatic patterns and local land-use changes. We argue that because the basis of the Budyko framework relies on the supply and demand concept, the framework could be effectively adapted and extended to quantify the role of drivers – both changing climate and local human disturbances – in altering the land-surface response across the globe. We review the Budyko framework, along with these potential extensions, with the intent of furthering the applicability of the framework to emerging hydrologic questions. Challenges in extending the Budyko framework over various spatio-temporal scales and the use of global datasets to evaluate the water balance at these various scales are also discussed.

Hydrology and Earth System Sciences

Coerced regimes: Management challenges in the Anthropocene

Management frequently creates system conditions that poorly mimic the conditions of a desirable self-organizing regime. Such management is ubiquitous across complex systems of people and nature and will likely intensify as these systems face rapid change. However, it is highly uncertain whether the costs (unintended consequences, including negative side effects) of management but also social dynamics can eventually outweigh benefits in the long term. We introduce the term “coerced regime” to conceptualize this management form and tie it into resilience theory. The concept encompasses proactive and reactive management to maintain desirable and mitigate undesirable regime conditions, respectively. A coerced regime can be quantified through a measure of the amount of management required to artificially maintain its desirable conditions. Coerced regimes comprise “ghosts” of self-sustaining desirable system regimes but ultimately become “dead regimes walking” when these regimes collapse as soon as management is discontinued. We demonstrate the broad application of coerced regimes using distinct complex systems of humans and nature (human subjects, aquatic and terrestrial environments, agriculture, and global climate). We discuss commonalities and differences between these examples to identify trade-offs between benefits and harms of management. The concept of coerced regimes can spur thinking and inform management about the duality of what we know and can envision versus what we do not know and therefore cannot envision: a pervasive sustainability conundrum as planet Earth swiftly moves toward a future without historical analogue

Ecology and Society

Tropical wetlands in the Anthropocene: The critical role of wet-dry cycles

In the face of climate change and increasing human water demands for agriculture, industry, and cities, the fate of wetland ecosystems in tropical wet-dry climates is threatened. To maximize biodiversity and ecological resilience, the value of the ecosystem services provided by tropical wetlands can be incorporated into regional land use and water management decisions. Environmental planners and resource managers can work to protect both the “dry” and “wet” phases of the wet-dry hydrologic cycles. These cycles have shaped and maintained these ecosystems in the past and they can be used to maximize biodiversity and resilience in the future.

Solutions Journal

Preface—Evaluating the response of critical zone processes to human impacts with sediment source fingerprinting

1) Background: Critical Zone Processes in the Anthropocene The Earth’s Critical Zone encompasses a suite of interconnected processes in the near-surface lithosphere, pedosphere, biosphere, atmosphere, and hydrosphere (Brantley et al., 2007; Lin, 2010) (Fig. 1). Processes and interactions both within and between these various Critical Zone components supports life-sustaining ecosystem services and resources that establish the foundation for humanity (NRC, 2001). This includes the formation production of fertile soils, flourishing vegetation, productive rivers, lakes and oceans, and our life-sustaining atmosphere (Gaillardet, 2014; Guo and Lin, 2016). Rapid population growth, land use intensification, and global environmental change are disturbing many of these fundamental Critical Zone processes. More than half of the Earth’s terrestrial surface is now impacted by anthropogenic activities (e.g., clearing, grazing, plowing, mining, and logging) (Hooke et al., 2012; Richter and Mobley, 2009). These changes are so widespread and pervasive that the great acceleration of socioeconomic development that occurred around 1950 (Fig. 2) has been recommended to delineate the dawn of the Anthropocene (Waters et al., 2016). Although the utility of adopting and delineating the Anthropocene as the current epoch is subject to debate (Crutzen, 2002; Ruddiman et al., 2015; Smith and Zeder, 2013), the concept effectively highlights both the nature and the extent of our global impact on Earth’s Critical Zone. Soil forming processes and ecosystem services provided by the pedosphere are central to the Critical Zone (Banwart et al., 2011; Lin, 2010). Many of these processes have been disturbed by the agricultural intensification that coincided with the great acceleration resulting in unsustainable land use practices now outpacing soil formation processes (Brantley et al., 2007). As agricultural landscapes now cover an area equivalent to what was scoured during the last glacial maximum (Amundson et al., 2007), the broad-scale intensification of anthropogenic activities has resulted in significant on- and off-site impacts. On-site, soil loss has resulted in decreases in soil fertility and agricultural yields (Ladha et al., 2009) threatening the ability to feed the world’s growing population (Brantley et al., 2007). Off-site, the excess delivery of particulate matter downstream is degrading riverine, lacustrine, and estuarine ecosystems (Bilotta and Brazier, 2008; Clark, 1985; Owens et al., 2005). The challenge, as noted by Brantley et al., (2007), is that despite our society having over 10,000 years of experience working with soils, our conceptual and quantitative models remain inadequate at predicting Critical Zone dynamics under current conditions. Notwithstanding growing pressure for improved environmental management, we still have a limited capacity to predict changes in the Critical Zone in response to anthropogenic activities owing to the multiple spatial and temporal scales at which these complex processes and feedbacks are manifest. As river basin systems are impacted by many of these processes, a deep understanding of soil-sediment continuum dynamics may provide a valuable framework for evaluating the disturbance response of Critical Zone processes. Understanding these processes may also provide land and resource managers with the information necessary to manage both the on-site and off-site effects of accelerated soil erosion.

Journal of Soils and Sediments

Bright spots for inland fish and fisheries to guide future hydropower development

Hydropower production is one of the greatest threats to fluvial ecosystems and freshwater biodiversity. Now that we have entered the Anthropocene , there is an opportunity to reflect on what might constitute a ‘sustainable’ Anthropocene in the context of hydropower and riverine fish populations. Considering elements of existing practices that promote favorable social-ecological outcomes (i.e., ‘bright spots’) is timely given that there are plans to expand hydropower capacity in previously undammed rivers, intensify dam development in some of the world's largest river systems, and re-license existing facilities. We approach this from a pragmatic perspective: for the foreseeable future, hydropower will likely remain an important source of renewable electricity. To offer support for moving toward a more ‘sustainable’ Anthropocene, we provide syntheses of best practices during the siting, design, construction, operation, and compensation phases of hydropower development to minimize impacts on inland fish. For each phase, we offer positive examples (or what might be considered ‘bright spots’) pertaining to some of the approaches described within our syntheses, acknowledging that these projects may not be viewed as without ecological and (or) societal detriment by all stakeholders. Our findings underscore the importance of protecting critical habitat and free-flowing river reaches through careful site selection and basin-scale planning, infrastructure designs that minimize reservoir effects and facilitate safe passage of fish, construction of hydropower plants using best practices that minimize long-term damage, operating guidelines that mimic natural flow conditions, and compensation that is lasting, effective, inclusive, and locally relevant. Learning from these ‘bright spots’ may require engagement of diverse stakeholders, professionals, and governments at scales that extend well beyond a given site, river, or even basin. Indeed, environmental planning that integrates hydropower development into broader discussions of conserving regional biodiversity and ecosystem services will be of utmost importance.

Water Biology and Security

Disease, environment, and pollution: Understanding drivers behind tumour outbreaks in sea turtles

Various wildlife diseases of the Anthropocene (the Anthropocene currently has no formal status in the Divisions of Geologic Time https://pubs.usgs.gov/fs/2018/3054/fs20183054.pdf , accessed 4 June 2024) have root causes that are found in human-driven environmental disturbances. Fibropapillomatosis of sea turtles is exemplary of a human-exacerbated wildlife disease, and this case study offers an overview of how we applied a One Health approach and interdisciplinary process to better understand its complexity and highlight the interconnections existing between the health of the environment, and of the wildlife and humans living in it. Persistent organic pollutants (POPs) have known detrimental effects on human and wildlife health. Ubiquitous in the environment, these degradation-resistant chemicals have a high bioaccumulation potential. This case study describes the interdisciplinary plan and One Health design implemented to measure the role of harmful pollutants in the occurrence of a marine turtle panzootic fibropapillomatosis (FP). FP is a neoplastic disease that causes the growth of debilitating tumours on soft tissues and internal organs. Disease incidence has been increasing significantly throughout the Anthropocene and the reasons are still uncertain. The pervasive effect of pollution in marine coastal habitats has often been hypothesized as a driver of high disease prevalence but never fully tested. Our project combines disease ecology, marine field biology, and chemical toxicology in the attempt to unravel the intricate dynamics behind FP. We here describe the complex process used to develop a methodology to measure levels of harmful seawater pollutants such as polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), and organochlorine pesticides (OCPs) in important sea turtle habitats in Florida. The proposed contaminants are among the top priority harmful pollutants, highly carcinogenic in aquatic animals, and bioaccumulate in sea turtles. Although only providing a description of the methodological process, this case study can help future research to apply similar transdisciplinary studies in the context of wildlife diseases. Understanding the effects of anthropogenic-driven pollution activity on ocean health can clarify its consequences to the health of wildlife and humans living in and around that environment.

One Health Cases

The effects of anthropogenic land cover change on pollen-vegetation relationships in the American Midwest

Fossil pollen assemblages provide information about vegetation dynamics at time scales ranging from centuries to millennia. Pollen-vegetation models and process-based models of dispersal typically assume stable relationships between source vegetation and corresponding pollen in surface sediments, as well as stable parameterizations of dispersal and productivity. These assumptions, however, are largely unevaluated. This paper reports a test of the stability of pollen-vegetation relationships using vegetation and pollen data from the Midwestern region of the United States, during a period of large changes in land use and vegetation driven by Euro-American settlement. We compared a dataset of pollen records for the early settlement-era with three other datasets of pollen and forest composition for two time periods: before Euro-American settlement, and the late 20th century. Results from generalized linear models for thirteen genera indicate that pollen-vegetation relationships significantly differ (p < 0.05) between pre-settlement and the modern era for several genera: Fagus, Betula, Tsuga, Quercus, Pinus , and Picea . The estimated pollen source radius for the 8 km gridded vegetation data and associated pollen data is 25–85 km, consistent with prior studies using similar methods and spatial resolutions. Hence, the rapid changes in land cover associated with the Anthropocene affect the accuracy of ecological predictions for both the future and the past. In the Anthropocene, paleoecology should move beyond the assumption that pollen-vegetation relationships are stable over time. Multi-temporal calibration datasets are increasingly possible and enable paleoecologists to better understand the complex processes governing pollen-vegetation relationships through space and time.

Michigan, Minnesota, Wisconsin

Stewardship and management of freshwater ecosystems: From Leopold's land ethic to a freshwater ethic

In 1949, Aldo Leopold formalized the concept of the ‘land ethic’, in what emerged as a foundational and transformational way of thinking about natural resource management, biodiversity conservation, and stewardship in terrestrial systems. Yet, the land ethic has inherent linkages to aquatic ecosystems; Leopold himself conducted research on rivers and lakes, and freshwater ecosystems figured widely in his writing. We reflect on the land ethic and other aspects of Leopold's scholarship to identify key messages that provide insight into the stewardship and management of freshwater ecosystems around the globe. We also frame what we call the ‘freshwater ethic’ around Leopold's legacy. Although Leopold could not have envisaged the stressors affecting modern aquatic ecosystems, his core principles remain salient. These apply not only to ecosystem protection, but also to the ethics of modern conservation economics, sustainability, and the protection of natural capital, in which lakes, rivers, and wetlands now figure prominently. We identify key ‘Aldo-inspired’ recommendations for protecting and restoring freshwater ecosystems in the Anthropocene that emanate directly from his writings (e.g. adopt an ecosystem approach, identify win–win–win scenarios, recognize the irreplaceability of wild waters, and strive for freshwater optimism). In an epoch where links between people and nature are becoming more explicit in environmental management, policy, and governance, we suggest that Aldo Leopold's work illustrates how inspirational, seminal thinkers have offered leadership in this domain. We contend that today there is still much that can be learned from Leopold, especially by the next generation of environmental practitioners, to ensure the effective stewardship of our aquatic ecosystems. We submit that the adoption of a freshwater ethic in parallel with Leopold's land ethic will enhance the stewardship of the world's increasingly threatened fresh waters by raising the profile of the plight of fresh waters and identifying enduring actions that, if embraced, will help conserve and restore biodiversity.

Aquatic Conservation: Marine and Freshwater Ecosys

Use of landscape simulation modeling to quantify resilience for ecological applications

Goals of fostering ecological resilience are increasingly used to guide U.S. public land management in the context of anthropogenic climate change and increasing landscape disturbances. There are, however, few operational means of assessing the resilience of a landscape or ecosystem. We present a method to evaluate resilience using simulation modeling. In this method, we use historical conditions (e.g., in North America, prior to European settlement), quantified using simulation modeling, to provide a comparative reference for contemporary conditions, where substantial departures indicate loss of resilience. Contemporary ecological conditions are compared statistically to the historical time series to create a resilience index, which can be used to prioritize landscapes for treatment and inform possible treatments. However, managing for resilience based on historical conditions is tenuous in the Anthropocene, which is characterized by rapid climate change, extensive human land use, altered disturbance regimes, and exotic species introductions. To account for the future variability of ecosystems resulting from climate and disturbance regime shifts, we augment historical simulations with simulations of ecosystem dynamics under projected climate and land use changes to assess the degree of departure from benchmark historical conditions. We use a mechanistic landscape model (FireBGCv2) applied to a large landscape in western Montana, USA, to illustrate the methods presented in this paper. Spatially explicit ecosystem modeling provides the vehicle to generate the historical and future time series needed to quantify potential resilience conditions associated with past and potential future conditions. Our methods show that given selection of a useful set of metrics, managers could use simulations like ours to evaluate potential future management directions.

Ecosphere

The unprecedented loss of Florida's reef-building corals and the emergence of a novel coral-reef assemblage

Over the last half century, climate change, coral disease, and other anthropogenic disturbances have restructured coral-reef ecosystems on a global scale. The disproportionate loss of once-dominant, reef-building taxa has facilitated relative increases in the abundance of “weedy” or stress-tolerant coral species. Although the recent transformation of coral-reef assemblages is unprecedented on ecological timescales, determining whether modern coral reefs have truly reached a novel ecosystem state requires evaluating the dynamics of reef composition over much longer periods of time. Here, we provide a geologic perspective on the shifting composition of Florida's reefs by reconstructing the millennial-scale spatial and temporal variability in reef assemblages using 59 Holocene reef cores collected throughout the Florida Keys Reef Tract (FKRT). We then compare the relative abundances of reef-building species in the Holocene reef framework to data from contemporary reef surveys to determine how much Florida's modern reef assemblages have diverged from long-term baselines. We show that the composition of Florida's reefs was, until recently, remarkably stable over the last 8000 yr. The same corals that have dominated shallow-water reefs throughout the western Atlantic for hundreds of thousands of years, Acropora palmata , Orbicella spp., and other massive coral taxa, accounted for nearly 90% of Florida's Holocene reef framework. In contrast, the species that now have the highest relative abundances on the FKRT, primarily Porites astreoides and Siderastrea siderea , were rare in the reef framework, suggesting that recent shifts in species assemblages are unprecedented over millennial timescales. Although it may not be possible to return coral reefs to pre-Anthropocene states, our results suggest that coral-reef management focused on the conservation and restoration of the reef-building species of the past, will optimize efforts to preserve coral reefs, and the valuable ecosystem services they provide into the future.

Florida

Witnessing history: Comparison of a century of sedimentary and written records in a California protected area

We use a combination of proxy records from a high-resolution analysis of sediments from Searsville Lake and adjacent Upper Lake Marsh and historical records to document over one and a half centuries of vegetation and socio-ecological change—relating to logging, agricultural land use change, dam construction, chemical applications, recreation, and other drivers—on the San Francisco Peninsula. A relatively open vegetation with minimal oak ( Quercus ) and coast redwood ( Sequoia sempervirens ) in the late 1850s reflects widespread logging and grazing during the nineteenth century. Forest and woodland expansion occurred in the early twentieth century, with forests composed of coast redwood and oak, among other taxa, as both logging and grazing declined. Invasive species include those associated with pasturage ( Rume x , Plantago ), landscape disturbance ( Urtica , Amaranthaceae), planting for wood production and wind barriers ( Eucalyptus ), and agriculture. Agricultural species, including wheat, rye, and corn, were more common in the early twentieth century than subsequently. Wetland and aquatic pollen and fungal spores document a complex hydrological history, often associated with fluctuating water levels, application of algaecides, raising of Searsville Dam, and construction of a levee. By pairing the paleoecological and historical records of both lakes, we have been able to reconstruct the previously undocumented impacts of socio-ecological influences on this drainage, all of which overprinted known climate changes. Recognizing the ecological manifestations of these impacts puts into perspective the extent to which people have interacted with and transformed the environment in the transition into the Anthropocene.

California

Forecasting natural regeneration of sagebrush after wildfires using population models and spatial matching

Context Addressing ecosystem degradation in the Anthropocene will require ecological restoration across large spatial extents. Identifying areas where natural regeneration will occur without direct resource investment will improve scalability of restoration actions. Objectives An ecoregion in need of large scale restoration is the Great Basin of the Western US, where increasingly large and frequent wildfires threaten ecosystem integrity and its foundational shrub species. We develop a framework to forecast where post-wildfire regeneration of sagebrush cover ( Artemisia spp.) is likely to occur within the burnt areas across the region (> 900,000 km 2 ). Methods First, we parameterized population models using Landsat satellite-derived time series of sagebrush cover. Second, we evaluated the out-of-sample performance by predicting natural regeneration in wildfires not used for model training. This model assessment reproduces a management-oriented scenario: making restoration decisions shortly after wildfires with minimal local information. Third, we asked how accounting for increasingly fine-scale spatial heterogeneity could improve model forecasting accuracy. Results Regional-level models revealed that sagebrush post-fire recovery is slow, estimating > 80-year time horizon to reach an average cover at equilibrium of 16.6% (CI95% 9–25). Accounting for wildfire and within-wildfire spatial heterogeneity improved out-of-sample forecasts, resulting in a mean absolute error of 3.5 ± 4.3% cover, compared to the regional model with an error of 7.2 ± 5.1% cover. Conclusions We demonstrate that combining population models and non-parametric spatial matching provides a flexible framework for forecasting plant population recovery. Models for population recovery applied to Landsat-derived time series will assist restoration decision-making, including identifying priority targets for restoration.

Great Basin

Resilience thinking for human-wildlife coexistence: Bridging dynamic systems, archetypes, and transformations

As human activities and wildlife increasingly overlap in the Anthropocene, conventional conservation paradigms focused on land-sparing are shifting toward strategies that support human–wildlife coexistence. However, achieving sustainable coexistence is often hindered by a limited understanding of the dynamic social–ecological processes that drive integrated human–wildlife systems. This Special Issue explores “resilience thinking” as a scientific framework to address these knowledge gaps. By bridging sustainability and wildlife sciences, we examine how concepts such as adaptive capacity, feedback loops, system archetypes, and tipping points can illuminate the conditions that facilitate stable coexistence or lead to persistent conflict. Through diverse global case studies ranging from carnivore management in Europe to primate interactions in Indonesia, contributions highlight how societal perceptions, governance, and co-adaptation shape system trajectories. Ultimately, we argue that fostering durable coexistence requires moving beyond incremental adaptation toward transformative governance that centers on equity, relationality, and proactive management.

Ambio

Spatial regimes provide ample early warning of tipping points

Accelerating global change is a hallmark of the Anthropocene, and the interaction of rapid change in climate, land use and land cover makes understanding the response of social-ecological systems to global change difficult to predict. Global change directly and indirectly affects both social-ecological systems and the landscapes in which they are embedded. Spatial heterogeneity in the location, manifestation of, and responses to global change makes spatially explicit approaches to management and conservation necessary. Spatial regimes, a concept derived from resilience theory, are at the forefront of attempts to operationalize and quantify resilience of dynamic landscapes. Spatial regimes are defined as dynamic landscape units that are shaped by a self-organizing set of processes and structures. They have identifiable spatial extents with discrete boundaries at a given scale that exhibit relative homogeneity in process, structure and composition maintained by feedback mechanisms. Here, we describe the concept of, evidence for, and applications of spatial regimes and how spatial regimes relate to scale and telecoupling of change across social-ecological systems. We emphasize the utility of the concept as an early warning of regime change, one that can provide ample early warning. We discuss methods that can be used to detect spatial regimes and uses of the concept for understanding and managing the spatio-temporal response of social-ecological systems to global change.

Advances in Ecological Research

Applied aspects of locomotion and biomechanics

Locomotion is the act and process of moving from place to place, which is fundamental to the life history of all mobile organisms. While the field of biomechanics encompasses the study of the physical constraints of what animals are capable of, ecological contexts require an integrated view that includes ecology and behavior. This chapter provides an overview of some of the areas where locomotion and biomechanics of fish movement interface with the rapidly evolving changes that humans impose on aquatic environments. These changes include fundamental alterations to the environment such as altered flows, fragmentation of riverine habitats, and invasive species, but also direct interactions that occur with capture fisheries. We explore each of these areas, considering both challenges and opportunities informed by the study of locomotion and biomechanics, emphasizing how this field can contribute to conservation of fishes in the Anthropocene. We then turn to technology, where important advances are aiding in our understanding of fish movement. In some cases those advances have themselves led to novel technologies, where biomimetic robots and related devices offer novel opportunities, both for conservation and for other pursuits.

Book chapter

Invasive species control and management: The sea lamprey story

Control of invasive species is a critical component of conservation biology given the catastrophic damage that they can cause to the ecosystems they invade. This is particularly evident with sea lamprey ( Petromyzon marinus ) in the Laurentian Great Lakes. Native to the Atlantic Ocean, the sea lamprey's ability to osmoregulate in fresh water, its wide thermal tolerance, generalist diet, and high fecundity allowed it to rapidly reach pest proportions in the prey-rich Great Lakes once it gained access through shipping canals. The invasion exacerbated declines in Great Lakes fisheries caused by overharvest, culminating in the crash of lake trout ( Salvelinus namaycush ) and other fish populations. In the last 60 years, however, a highly successful sea lamprey control program has reduced sea lamprey to ∼10% of their peak abundance and has been instrumental in enabling the rehabilitation of the Great Lakes ecosystem. In this chapter, we: (1) discuss the likely vectors of the invasion and the physiological attributes of sea lamprey that enabled them to become established in the Great Lakes; (2) review the two cornerstones of the sea lamprey control program—which relies on a combination of pesticides to eradicate multiple generations of larval sea lamprey in their nursey streams, and in-stream barriers to restrict the upstream migration of spawning lamprey—both of which exploit unique physiological vulnerabilities of sea lamprey; (3) describe how sea lamprey control can adversely affect non-target species and how these can be mitigated; (4) show how physiology-based approaches are improving our understanding of the lethal and sublethal effects of sea lamprey on host fishes; and (5) discuss the future of conservation physiology in sea lamprey control. The prime challenge in the next several decades of the Anthropocene will be to further refine the specificity of control tools while maintaining their efficacy, and to adapt to a warming climate and other anthropogenic activities affecting the Great Lakes and their tributaries.

Lake Erie, Lake Huron, Lake Michigan, Lake Ontario

Turtle biogeography: Global regionalization and conservation priorities

Defaunation in the Anthropocene has created a need to focus limited conservation resources on geographically-explicit areas with high conservation significance. Priority conservation areas are often defined as those with high biodiversity – hotspots. While these conservation areas are critical to securing global biodiversity, prevailing approaches for their delineation are often qualitative. Here, we demonstrate the benefits of a clade-specific approach that improves conservation. We use the distinct, imperiled clade of turtles (tortoises and freshwater turtles) to delineate biogeographical regions and characterize their comparative levels of biodiversity and conservation values. We produce a quantitative, revisable map of 63 global turtle regions and identify several turtle regions, mostly in the Indomalayan turtle realm, that are high-priority conservation regions. While our high-priority turtle regions include those previously reported in the literature as turtle hotspots, we also describe a new priority conservation region in Southeast Asia (Yangtze-Huang He-Xi Yiang) which has both high species and endemism richness. Although not considered a high or intermediate conservation priority, our analyses delineated another previously unidentified turtle hotspot – the Kalahari Basin-Rift Valley. Additionally, we identify several turtle regions, largely in Central America, with intermediate conservation priority. Our results reveal that many turtle hotspots represent complex biogeographical areas with high inter-regional β -diversity, and several of these turtle-hotspots occur in transition zones with high biogeographical complexity. In these cases, inter-regional conservation efforts will be necessary to ensure turtle biodiversity conservation.

Biological Conservation