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Methods of Data Collection, Sample Processing, and Data Analysis for Edge-of-Field, Streamgaging, Subsurface-Tile, and Meteorological Stations at Discovery Farms and Pioneer Farm in Wisconsin, 2001-7

The University of Wisconsin (UW)-Madison Discovery Farms (Discovery Farms) and UW-Platteville Pioneer Farm (Pioneer Farm) programs were created in 2000 to help Wisconsin farmers meet environmental and economic challenges. As a partner with each program, and in cooperation with the Wisconsin Department of Natural Resources and the Sand County Foundation, the U.S. Geological Survey (USGS) Wisconsin Water Science Center (WWSC) installed, maintained, and operated equipment to collect water-quantity and water-quality data from 25 edge-offield, 6 streamgaging, and 5 subsurface-tile stations at 7 Discovery Farms and Pioneer Farm. The farms are located in the southern half of Wisconsin and represent a variety of landscape settings and crop- and animal-production enterprises common to Wisconsin agriculture. Meteorological stations were established at most farms to measure precipitation, wind speed and direction, air and soil temperature (in profile), relative humidity, solar radiation, and soil moisture (in profile). Data collection began in September 2001 and is continuing through the present (2008). This report describes methods used by USGS WWSC personnel to collect, process, and analyze water-quantity, water-quality, and meteorological data for edge-of-field, streamgaging, subsurface-tile, and meteorological stations at Discovery Farms and Pioneer Farm from September 2001 through October 2007. Information presented includes equipment used; event-monitoring and samplecollection procedures; station maintenance; sample handling and processing procedures; water-quantity, waterquality, and precipitation data analyses; and procedures for determining estimated constituent concentrations for unsampled runoff events.

Open-File Report↗

Echelon approach to areas of concern in synoptic regional monitoring

Echelons provide an objective approach to prospecting for areas of potential concern in synoptic regional monitoring of a surface variable. Echelons can be regarded informally as stacked hill forms. The strategy is to identify regions of the surface which are elevated relative to surroundings ( R elative ELEVATIONS or RELEVATIONS ). These are areas which would continue to expand as islands with receding (virtual) floodwaters. Levels where islands would merge are critical elevations which delimit echelons in the vertical dimension. Families of echelons consist of surface sectors constituting separate islands for deeper waters that merge as water level declines. Pits which would hold water are disregarded in such a progression, but a complementary analysis of pits is obtained using the surface as a virtual mould to cast a counter-surface (bathymetric analysis). An echelon tree is a family tree of echelons with peaks as terminals and the lowest level as root. An echelon tree thus provides a dendrogram representation of surface topology which enables graph theoretic analysis and comparison of surface structures. Echelon top view maps show echelon cover sectors on the base plane. An echelon table summarizes characteristics of echelons as instances or cases of hill form surface structure. Determination of echelons requires only ordinal strength for the surface variable, and is thus appropriate for environmental indices as well as measurements. Since echelons are inherent in a surface rather than perceptual, they provide a basis for computer-intelligent understanding of surfaces. Echelons are given for broad-scale mammalian species richness in Pennsylvania.

Environmental and Ecological Statistics↗

Method to support Total Maximum Daily Load development using hydrologic alteration as a surrogate to address aquatic life impairment in New Jersey streams

More than 300 ambient monitoring sites in New Jersey have been identified by the New Jersey Department of Environmental Protection (NJDEP) in its integrated water-quality monitoring and assessment report (that is, the 305(b) Report on general water quality and 303(d) List of waters that do not support their designated uses) as being impaired with respect to aquatic life; however, no unambiguous stressors (for example, nutrients or bacteria) have been identified. Because of the indeterminate nature of the broad range of possible impairments, surrogate measures that more holistically encapsulate the full suite of potential environmental stressors need to be developed. Streamflow alteration resulting from anthropogenic changes in the landscape is one such surrogate. For example, increases in impervious surface cover (ISC) commonly cause increases in surface runoff, which can result in “flashy” hydrology and other changes in the stream corridor that are associated with streamflow alteration. The NJDEP has indicated that methodologies to support a hydrologically based Total Maximum Daily Load (hydro-TMDL) need to be developed in order to identify hydrologic targets that represent a minimal percent deviation from a baseline condition (“minimally altered”) as a surrogate measure to meet criteria in support of designated uses. The primary objective of this study was to develop an applicable hydro-TMDL approach to address aquatic-life impairments associated with hydrologic alteration for New Jersey streams. The U.S. Geological Survey, in cooperation with the NJDEP, identified 51 non- to moderately impaired gaged streamflow sites in the Raritan River Basin for evaluation. Quantile regression (QR) analysis was used to compare flow and precipitation records and identify baseline hydrographs at 37 of these sites. At sites without an appropriately long period of record (POR) or where a baseline hydrograph could not be identified with QR, a rainfall-runoff model was used to develop simulated baseline hydrographs. The hydro-TMDL approach provided an opportunity to evaluate proportional differences in flow attributes between observed and baseline hydrographs and to develop complementary flow-ecology response relations at a subset of Raritan River Basin sites where available flow and ecological information overlapped. The New Jersey Stream Classification Tool (NJSCT) was used to determine the stream class of all 51 study sites by using either an observed or a simulated baseline hydrograph. Two New Jersey stream classes (A and C) were evaluated to help characterize the unique hydrology of the Raritan River Basin. In general, class C streams (1.99–40.7 square miles) had smaller drainage areas than class A streams (0.7–785 square miles). Many of the non-impaired and moderately impaired class A and C streams in the Raritan River Basin were found to have significant hydrologic alteration as indicated by numerous flow values that fell outside the established 25th-to-75th- and the more conservative 40th-to-60th-percentile boundaries. However, percent deviations for the class C streams (defined as moderately stable streams with moderately high base-flow contributions) were, in general, much larger than those for the class A streams (defined as semiflashy streams characterized by moderately low base flow). The greater deviations for class C streams in the hydro-TMDL assessments likely resulted from comparisons that were based solely on simulated baseline hydrographs, which were developed without considering any anthropogenic influences in the basin. In contrast, comparisons for many of the class A streams were made by using an observed baseline, which already includes an implicit level of ISC and other human influences on the landscape. By using the hydro-TMDL approach, numerous flow deviations were identified that were indicative of streams that are highly regulated by reservoirs or dams, streams that are affected by increasing amounts of surface runoff resulting from ISC, and streams that are affected by water abstraction (that is, groundwater or surface-water withdrawals used for agricultural and human supply). Eight of the reservoir- and (or) dam-affected sites showed flow deviations that are indicative of flow-managed systems. For example, indices that account for the timing and magnitude of high and low flows were often found to fall outside the 25th-to-75th-percentile range. In general, at regulated class C streams, annual summer low flows are arriving later and tend to be lower, and high flows are arriving earlier with higher magnitudes of longer duration. At class A streams, high and low flows are arriving later with an overall increase in discharge with respect to the prereservoir baseline conditions. The drainage basins of eight of the study sites had large values of ISC (>10 percent), most likely as a result of expanding urban development. In general, the magnitude and frequency of high flows at class A and C sites with high ISC are increasing and were commonly found to fall outside the 25th-to-75th-percentile range. Additionally, magnitudes of low flows are becoming lower and, although the timing of high flows was highly variable, low-flow events appeared to be arriving earlier than would be expected under normal low-flow conditions. Three of the study sites appeared to be affected by hydrologic changes associated with water abstraction. At these sites, the timing of flows appeared to be altered. For example, low flows tended to arrive earlier and high flows arrived later at two of the three sites. Additionally, the magnitude and duration of low flows were commonly less than the 25th-percentile value and the duration of high flows appeared to increase. A reduced set of hydrologic and ecological variables was used to develop univariate and multivariate flow-ecology response models for the aquatic-invertebrate assemblage. Many hydrologic variables accounting for the duration, magnitude, frequency, and timing of flows were significantly correlated with ecological response. Multiple linear regression (MLR) models were developed to provide a more holistic evaluation of the combined effects of hydrologic alteration and to identify models with two or three hydrologic variables that account for a significant proportion of the variability in invertebrate-assemblage condition as represented by assemblage metric scores. MLR models, derived on the basis of hydrologic attributes, accounted for 35 to 75 percent of the variability in assemblage condition. The hydro-TMDL method developed herein for non- to moderately impaired Raritan River Basin streams utilizes a “surrogate” approach in place of the traditional “pollutant of concern” approach commonly used for TMDL development. Managers can use the results obtained by using the hydro-TMDL method to offset the effects of impervious-surface runoff and altered streamflow and to implement measures designed to achieve the necessary load reductions for the “pollutant of concern” (that is, percentage deviations of stream-class-specific flow-index values outside the established 25th-to-75th-percentile range). In this case, such deviations could represent all or a subset of the altered flow indices that prevent the stream from meeting designated aquatic-life criteria. This hydro-TMDL uses a reference, or attainment stream approach for developing the TMDL endpoint. That is, either observed or simulated baseline hydrographs were selected as appropriate reference conditions on the basis of results of QR analysis and watershed modeling procedures, respectively. For any stream in the Raritan River Basin evaluated as part of this study, the hydro-TMDL can be expressed as the greatest amount of deviation in flow a stream can exhibit without violating the stream’s designated aquatic-life criteria. Use of this surrogate approach is appropriate because flows that fall outside the established percentile ranges are ultimately a function of many anthropogenic modifications of the landscape, including the amount of stormwater runoff generated from impervious surfaces within a given basin, the presence of manmade structures designed to retain or divert water, the magnitude of ground- and surface-water abstraction, and the presence of water-supply processes implemented to support human needs. In addition, the stream-type-specific flow indices used as the basis for the hydro-TMDL approach are useful for representing the hydrologic conditions of class A and C streams/basins because they incorporate the full spectrum of flow conditions (very low to very high) that occur in the stream system over a long period of time, as well as those flow properties that change as a result of seasonal variation. Ultimately, an estimate of the maximum percentage flow reduction that could be allowed will be needed to address the aquatic-life impairments in many of the study streams in the Raritan River Basin and will be necessary for identifying appropriate target flow conditions for hydro-TMDL implementation. As described in this report, a target flow value equal to the 25th- or 75th-percentile flow rate could be selected as the point useful for setting specific hydrologic targets. This selection, however, is a management decision that could vary depending on the designated use of the stream or other regulatory factors (for example, water-supply protection, trout production, antidegradation policies, or special protection designations). In New Jersey streams where no unambiguous stressors can be identified, State monitoring agencies, such as the NJDEP, could choose to require the implementation of a flow-based TMDL that not only supports designated uses, but meets the regulatory requirements under the Clean Water Act, and represents a balance between water supply intended to meet human needs and the conservation of ecosystem integrity.

New Jersey↗

Influence of season, sex, age and diet composition on mercury concentration in Walleye Sander vitreus

We collected Walleye Sander vitreus (May–October) from Bitter and Twin lakes, South Dakota to assess seasonal- and diet-related variation in tissue mercury (Hg) concentration. The average Hg concentration in Walleye was 43–68% higher in the spring for Bitter ( p < 0.008) and Twin Lakes ( p < 0.017) compared with summer or autumn months. Bioenergetics analysis of Bitter Lake Walleye showed that consumption of fish prey (primarily Fathead Minnow Pimephales promelas ) increased from late summer through winter and was linked to increased Hg accumulation in Walleye the following spring. Mercury concentration varied significantly with Walleye age but was similar for comparably-aged male (0.62 μg/g) and female fish (0.62 μg/g). However, after adjusting for Walleye size (total length, mm), mean Hg concentration was greater in male (0.66 μg/g) compared with female (0.50 μg/g) fish, likely due to slower growth rate of male Walleye. At 425 mm, male Walleye in Bitter Lake were approximately 1 year older than female fish. These findings show that diet, age, and gender-related growth affect Hg concentration in Walleye and are important factors to consider in fish contaminant monitoring programs.

South Dakota↗

A rapid assessment method to estimate the distribution of juvenile Chinook Salmon in tributary habitats using eDNA and occupancy estimation

Identification and protection of water bodies used by anadromous species are critical in light of increasing threats to fish populations, yet often challenging given budgetary and logistical limitations. Noninvasive, rapid‐assessment, sampling techniques may reduce costs and effort while increasing species detection efficiencies. We used an intrinsic potential (IP) habitat model to identify high‐quality rearing habitats for Chinook Salmon Oncorhynchus tshawytscha and select sites to sample throughout the Chena River basin, Alaska, for juvenile occupancy using an environmental DNA (eDNA) approach. Water samples were collected from 75 tributary sites in 2014 and 2015. The presence of Chinook Salmon DNA in water samples was assessed using a species‐specific quantitative PCR (qPCR) assay. The IP model predicted over 900 stream kilometers in the basin to support high‐quality (IP ≥ 0.75) rearing habitat. Occupancy estimation based on eDNA samples indicated that 80% and 56% of previously unsampled sites classified as high or low IP (IP < 0.75), respectively, were occupied. The probability of detection ( p ) of Chinook Salmon DNA from three replicate water samples was high ( p = 0.76) but varied with drainage area (km 2 ). A power analysis indicated high power to detect proportional changes in occupancy based on parameter values estimated from eDNA occupancy models, although power curves were not symmetrical around zero, indicating greater power to detect positive than negative proportional changes in occupancy. Overall, the combination of IP habitat modeling and occupancy estimation provided a useful, rapid‐assessment method to predict and subsequently quantify the distribution of juvenile salmon in previously unsampled tributary habitats. Additionally, these methods are flexible and can be modified for application to other species and in other locations, which may contribute towards improved population monitoring and management.

Alaska↗

BRIDGES: Evolution of basic and applied linkages in benthic science

Growing awareness of environmental degradation resulted in stricter environmental regulations and laws for aquatic ecosystems. These regulations were followed by an increase in applied research and monitoring beginning in the early 1970s. The number of applied scientists who were members of the North American Benthological Society grew at a commensurate rate. The editors of J-NABS recognized that, despite these increases, submitted manuscripts mostly addressed basic science. In response, the BRIDGES section of J-NABS was created in 1994 to provide a forum for linking basic ecological principles to applied science problems and issues. We examined the emergence of applied science topics in J-NABS and its predecessor, Freshwater Invertebrate Biology , from their beginning in 1982 to 2009. We classified papers among 11 categories that included a basic/applied science linkage. In the 1980s, applied papers were predominantly on effects of eutrophication/pollution and landuse changes. When BRIDGES was established in 1994, papers were solicited by editors and BRIDGES sections usually included >1 paper on a common theme to express complementary or alternate viewpoints. Forty-two papers appeared in BRIDGES between 1994 and 2009, but the number per issue declined after 2001. The total number of applied science papers in J-NABS has increased since ∼1994. Citation analysis of BRIDGES papers illustrates how information is being cited, but applied papers often are used in ways that might not lead to citations. BRIDGES transitioned to a new format in September 2009 to address new types of complex, multifaceted linkages. All new BRIDGES articles will be open access, and authors will be encouraged to produce lay-language fact sheets and to post them on the web.

Journal of the North American Benthological Societ↗

Correlating sea lamprey density with environmental DNA detections in the lab

Invasive sea lamprey (Petromyzon marinus Linnaeus, 1758) are currently managed by the Great Lakes Fishery Commission in an effort to reduce pest populations below levels that cause ecological damage. One technique to improve stream population assessments could be molecular surveillance in the form of environmental DNA (eDNA) monitoring. We developed and validated four probe-based quantitative polymerase chain reaction (qPCR) assays, then used two probes (cytb, nd1) to determine whether eDNA concentration was correlated with adult and larval sea lamprey density in the lab. We found a strong positive correlation between adult sea lamprey densities of 2, 20, and 200 individuals/2000L and eDNA concentrations in tanks using both assays (cytb, nd1). For larval laboratory tank density trials, eDNA concentrations were generally near our limit of quantification and there was no significant difference in copy numbers detected between larval sea lamprey densities of 1, 5, and 25 individuals/28L. Therefore, we examined detection probability rather than concentration with laboratory tank densities. We observed a trend of increasing detection probabilities with increased larval sea lamprey density that approached significance suggesting that when DNA copy numbers are low, detection rates may be more informative in predicting varying densities of larval sea lamprey. The ability to assess sea lamprey densities from a water sample could be a powerful tool to improve traditional assessment and stream ranking techniques. Further refinement of this method in the field may make eDNA surveillance of sea lamprey a reliable part of stream assessments. Rapid eDNA analysis from many streams may help focus traditional assessment efforts, thereby improving the efficiency of invasive sea lamprey control efforts.

Management of Biological Invasions↗

Pesticides in the hydrologic system - What do we know and what's next?

Even though the occurrence and behaviour of pesticides in the environment have been studied for decades, water-quality managers and the public still demand more complete and consistent information, and there are many unanswered questions for environmental scientists. In many respects, the greatest potential for unintended adverse effects of pesticides is through contamination of the hydrologic system, which supports aquatic life and related food chains and is used for recreation, drinking water, and many other purposes. The movement of water is one of the primary mechanisms by which pesticides are transported from targeted application areas to other parts of the environment; thus, there is potential for movement into and through all components of the hydrologic system. Extensive reviews of existing information on pesticides in the hydrologic system, including the atmosphere (Majewski and Capel, 1995), ground water (Barbash and Resek, 1996), surface water (Larson et al., 1997), and fluvial sediments and aquatic biota (Nowell et al., 1999), uncovered volumes of useful information, but also noted critical information gaps. For example: (a) relatively few pesticides have been thoroughly studied, particularly transformation products; (b) most data have been collected for small-scale site and field studies in agricultural areas; (c) urban areas have received little attention for monitoring or research; (d) the geographic and temporal distributions of data collection have been highly uneven; and (e) comparing and synthesizing results from most studies is difficult because of inconsistent approaches to data collection and chemical analysis.

Hydrological Processes↗

A centroid model of species distribution with applications to the Carolina wren Thryothorus ludovicianus and house finch Haemorhous mexicanus in the United States

Drastic shifts in species distributions are a cause of concern for ecologists. Such shifts pose great threat to biodiversity especially under unprecedented anthropogenic and natural disturbances. Many studies have documented recent shifts in species distributions. However, most of these studies are limited to regional scales, and do not consider the abundance structure within species ranges. Developing methods to detect systematic changes in species distributions over their full ranges is critical for understanding the impact of changing environments and for successful conservation planning. Here, we demonstrate a centroid model for range-wide analysis of distribution shifts using the North American Breeding Bird Survey. The centroid model is based on a hierarchical Bayesian framework which models population change within physiographic strata while accounting for several factors affecting species detectability. Yearly abundance-weighted range centroids are estimated. As case studies, we derive annual centroids for the Carolina wren and house finch in their ranges in the U.S. We further evaluate the first-difference correlation between species’ centroid movement and changes in winter severity, total population abundance. We also examined associations of change in centroids from sub-ranges. Change in full-range centroid movements of Carolina wren significantly correlate with snow cover days (r = −0.58). For both species, the full-range centroid shifts also have strong correlation with total abundance (r = 0.65, and 0.51 respectively). The movements of the full-range centroids of the two species are correlated strongly (up to r = 0.76) with that of the sub-ranges with more drastic population changes. Our study demonstrates the usefulness of centroids for analyzing distribution changes in a two-dimensional spatial context. Particularly it highlights applications that associate the centroid with factors such as environmental stressors, population characteristics, and progression of invasive species. Routine monitoring of changes in centroid will provide useful insights into long-term avian responses to environmental changes.

Ecography↗

Linking spring phenology with mechanistic models of host movement to predict disease transmission risk

Disease models typically focus on temporal dynamics of infection, while often neglecting environmental processes that determine host movement. In many systems, however, temporal disease dynamics may be slow compared to the scale at which environmental conditions alter host space-use and accelerate disease transmission. Using a mechanistic movement modelling approach, we made space-use predictions of a mobile host (elk [ Cervus Canadensis ] carrying the bacterial disease brucellosis) under environmental conditions that change daily and annually (e.g., plant phenology, snow depth), and we used these predictions to infer how spring phenology influences the risk of brucellosis transmission from elk (through aborted foetuses) to livestock in the Greater Yellowstone Ecosystem. Using data from 288 female elk monitored with GPS collars, we fit step selection functions (SSFs) during the spring abortion season and then implemented a master equation approach to translate SSFs into predictions of daily elk distribution for five plausible winter weather scenarios (from a heavy snow, to an extreme winter drought year). We predicted abortion events by combining elk distributions with empirical estimates of daily abortion rates, spatially varying elk seroprevelance and elk population counts. Our results reveal strong spatial variation in disease transmission risk at daily and annual scales that is strongly governed by variation in host movement in response to spring phenology. For example, in comparison with an average snow year, years with early snowmelt are predicted to have 64% of the abortions occurring on feedgrounds shift to occurring on mainly public lands, and to a lesser extent on private lands. Synthesis and applications . Linking mechanistic models of host movement with disease dynamics leads to a novel bridge between movement and disease ecology. Our analysis framework offers new avenues for predicting disease spread, while providing managers tools to proactively mitigate risks posed by mobile disease hosts. More broadly, we demonstrate how mechanistic movement models can provide predictions of ecological conditions that are consistent with climate change but may be more extreme than has been observed historically.

Journal of Applied Ecology↗

Analysis of surface water trends for the conterminous United States using MODIS satellite data, 2003–2019

Satellite imagery is commonly used to map surface water extents over time, but many approaches yield discontinuous records resulting from cloud obstruction or image archive gaps. We applied the Dynamic Surface Water Extent (DSWE) model to downscaled (250-m) daily Moderate Resolution Imaging Spectroradiometer (MODIS) data in Google Earth Engine to generate monthly surface water maps for the conterminous United States (US) from 2003 through 2019. The aggregation of daily observations to monthly maps of maximum water extent produced records with diminished cloud and cloud shadow effects across most of the country. We used the continuous monthly record to analyze spatiotemporal surface water trends stratified within Environmental Protection Agency Ecoregions. Although not all ecoregion trends were significant ( p < 0.05), results indicate that much of the western and eastern US underwent a decline in surface water over the 17-year period, while many ecoregions in the Great Plains had positive trends. Trends were also generated from monthly streamgage discharge records and compared to surface water trends from the same ecoregion. These approaches agreed on the directionality of trend detected for 54 of 85 ecoregions, particularly across the Great Plains and portions of the western US, whereas trends were not congruent in select western deserts, the Great Lakes region, and the southeastern US. By describing the geographic distribution of surface water over time and comparing these records to instrumented discharge data across the conterminous US, our findings demonstrate the efficacy of using satellite imagery to monitor surface water dynamics and supplement traditional instrumented monitoring.

Water Resources Research↗

Use of field-applied quality control samples to monitor performance of a Goulden large-sample extractor/GC-MS method for pesticides in water

Since 1985, the Goulden large-sample extractor (GLSE) has been used to isolate a broad array of trace-organic contaminants from large volumes of water. In this study, field-applied quality control measures, including matrix and surrogate spikes and blanks, were used to monitor method performance from GLSE extraction through GC-MS analysis. The method was applied to the determination of multiple classes of pesticides isolated from 4- to 112-L filtered surface-water samples. Average recoveries of six surrogate compounds ranged from 84 ?? 18% for [2H10]diazinon to 15 ?? 13% for 4,4'-[2H8]DDT, the low recoveries for which were largely a result of unmonitored breakdown of this surrogate by the GC injection system. Field-matrix-spike samples were prepared by fortifying 10-L, 35-L, and 110-L filtered surface-water samples with 68 pesticides to amended concentrations of 11- to 50-ng/L each. Recoveries ranged from not detected to greater than 100%. Variability in pesticide recoveries from triplicate 10-L water samples collected at one site averaged 5.7% relative standard deviation and did not exceed 19%.Since 1985, the Goulden large-sample extractor (GLSE) has been used to isolate a broad array of trace-organic contaminants from large volumes of water. In this study, field-applied quality control measures, including matrix and surrogate spikes and blanks, were used to monitor method performance from GLSE extraction through GC-MS analysis. The method was applied to the determination of multiple classes of pesticides isolated from 4- to 112-L filtered surface-water samples. Average recoveries of six surrogate compounds ranged from 84 ?? 18% for [2H10]diazinon to 15 ?? 13% for 4,4???-[2H8]DDT, the low recoveries for which were largely a result of unmonitored breakdown of this surrogate by the GC injection system. Field-matrix-spike samples were prepared by fortifying 10-L, 35-L, and 110-L filtered surface-water samples with 68 pesticides to amended concentrations of 11- to 50-ng/L each. Recoveries ranged from not detected to greater than 100%. Variability in pesticide recoveries from triplicate 10-L water samples collected at one site averaged 5.7% relative standard deviation and did not exceed 19%.

International Journal of Environmental Analytical ↗

Avian malaria in a boreal resident species: long-term temporal variability, and increased prevalence in birds with avian keratin disorder

The prevalence of vector-borne parasitic diseases is widely influenced by biological and ecological factors. Environmental conditions such as temperature and precipitation can have a marked effect on haemosporidian parasites ( Plasmodium spp.) that cause malaria and those that cause other malaria-like diseases in birds. However, there have been few long-term studies monitoring haemosporidian infections in birds in northern latitudes, where weather conditions can be highly variable and the effects of climate change are becoming more pronounced. We used molecular methods to screen more than 2,000 blood samples collected from black-capped chickadees ( Poecile atricapillus ), a resident passerine bird. Samples were collected over a 10 year period, mostly during the non-breeding season, at seven sites in Alaska, USA. We tested for associations between Plasmodium prevalence and local environmental conditions including temperature, precipitation, site, year and season. We also evaluated the relationship between parasite prevalence and individual host factors of age, sex and presence or absence of avian keratin disorder. This disease, which causes accelerated keratin growth in the beak, provided a natural study system in which to test the interaction between disease state and malaria prevalence. Prevalence of Plasmodium infection varied by year, site, age and individual disease status but there was no support for an effect of sex or seasonal period. Significantly, birds with avian keratin disorder were 2.6 times more likely to be infected by Plasmodium than birds without the disorder. Interannual variation in the prevalence of Plasmodium infection at different sites was positively correlated with summer temperatures at the local but not statewide scale. Sequence analysis of the parasite cytochrome b gene revealed a single Plasmodium spp. lineage, P43. Our results demonstrate associations between prevalence of avian malaria and a variety of biological and ecological factors. These results also provide important baseline data that will be informative for predicting future changes in Plasmodium prevalence in the subarctic.

Alaska↗

Application of transcriptomics concentration-response modeling for prioritization of contaminants detected in tributaries of the North American Great Lakes

As part of the Great Lakes Restoration Initiative, chemical monitoring and surveillance efforts have detected approximately 330 chemicals in surface water of Great Lakes tributaries. There were 140 chemicals for which no empirical toxicity data were available. The aim of this study was to generate transcriptomic points of departure (tPODs) for 10 of these compounds and demonstrate how they could be applied in a screening-level prioritization. Organisms representing three trophic levels of the aquatic food web ( Pimephales promelas , Daphnia magna , and Raphidocelis subcapitata ) were exposed for 24 hr to a half-log dilution series of nominal exposure concentrations typically ranging from 66.7–0.021 µM of each chemical. In addition to observations of apical effects (e.g., survival and morphology), whole body transcriptomic responses (tPODs) to each chemical were evaluated with targeted analysis using TempO-seq for P. promelas and D. magna and nontargeted RNA-seq for R. subcapitata . The tPODs ranged from 0.18–10.8 µM for P. promelas and 0.32–29 µM for D. magna , with the most potent of the chemicals tested being fipronil carboxamide for both species. For R. subcapitata , the tPODs ranged from 0.04–1.77 µM, with gabapentin as the most potent chemical tested. Empirically derived tPODs from these data-poor chemicals were compared with concentrations detected in the Great Lakes basin. Environmental concentrations were less than the tPODs except for R. subcapitata and 3,4-dichlorophenyl isocyanate. Similarly, tPODs from previously tested data-rich chemicals were compared with environmental concentrations, in which case tPODs from several chemicals overlapped environmental concentrations. This work demonstrates the potential utility of emerging ecological high-throughput transcriptomics assays to support screening and prioritization of data-poor environmental contaminants.

Environmental Toxicology and Chemistry↗

Implementation of a non-lethal biopsy punch monitoring program for mercury in smallmouth bass, Micropterus dolomieu Lacepede, from the Eleven Point River, Missouri

A non-lethal biopsy method for monitoring mercury (Hg) concentrations in smallmouth bass ( Micropterus dolomieu ; smallmouth) from the Eleven Point River in southern Missouri USA was evaluated. A biopsy punch was used to remove a muscle tissue plug from the area immediately below the anterior dorsal fin of 31 smallmouth. An additional 35 smallmouth (controls) were held identically except that no tissue plug was removed. After sampling, all fish were held in a concrete hatchery raceway for 6 weeks. Mean survival at the end of the holding period was 97 % for both groups. Smallmouth length, weight and Fulton’s condition factor at the end of the holding period were also similar between plugged and non-plugged controls, indicating that the biopsy procedure had minimal impact on growth under these conditions. Tissue plug Hg concentrations were similar to smallmouth Hg data obtained in previous years by removing the entire fillet for analysis.

Missouri↗

Baseline water-quality inventory and summary for the Paterson Great Falls National Historical Park, New Jersey

The Paterson Great Falls National Historical Park (PAGR), established in 2011 in the City of Paterson, New Jersey, contains many cultural, historical, and natural resources. Because of the PAGR’s young age and limited resources, a long record of natural resource data is not available for use to guide future park management and planning decisions. A detailed natural resource inventory was identified as an immediate need in the “Foundation Document—Paterson Great Falls National Historical Park,” given the history of extensive industrial activity throughout the PAGR and the Passaic River Basin. The U.S. Geological Survey, in cooperation with the National Park Service and the Urban Waters Federal Partnership for the Lower Passaic River, inventoried water-quality monitoring efforts and compiled historical water-quality data from secondary data sources to define baseline conditions in the PAGR and the surrounding study area. This report documents the methods used to harmonize water-quality data aggregated from multiple sources for the PAGR study area. Procedures implemented during data harmonization include compiling data from multiple sources, assessing the dataset for completeness, addressing metadata concerns, and creating a consistent and quality-assured dataset for future analysis. The harmonized dataset contains 14 aggregated surface-water sites, 1,103 individual sampling days, and 32,000 water-quality records throughout the period of record defined for this study (1952–2021). Summary statistics and comparison to water-quality criteria are provided for focus parameters that can support PAGR management, including field properties, suspended material, total dissolved solids, specific conductance, chloride, sulfate, Escherichia coli, nutrients, contaminants of concern related to fish consumption advisories, and litter and floatable debris. A spatial data gap was identified for the recreational area near the base of the Great Falls and immediately downstream from a known combined sewer overflow discharge location. Analytic data gaps were identified for continuous streamflow and water-quality parameters, floatable debris characterization, and harmful algal blooms identification. Continuous collection of contaminants of concern was identified as a temporal data gap, as these parameters were not consistently collected within the study area. The water-quality data synthesis and summary presented in this report can assist PAGR staff in understanding the complex baseline water resources within the study area and can serve as a baseline to design future water-quality monitoring efforts and evaluate changes in water quality over time. The harmonized dataset, as well as future New Jersey Department of Environmental Protection and New Jersey Harbor Discharge Group monitoring data and assessments, can also be used to examine the status and trends in surface-water quality, support compliance with the Clean Water Act (33 U.S.C. 1251 et seq.), and further investigate changes to park water resources that can affect ecological and human health.

New Jersey↗

Quality of shallow ground water in areas of recent residential and commercial development, Wichita, Kansas, 2000

Water samples from 30 randomly distributed monitoring wells in areas of recent residential and commercial development (1960–96), Wichita, Kansas, were collected in 2000 as part of the High Plains Regional Ground-Water Study conducted by the U.S. Geological Survey's National Water-Quality Assessment (NAWQA) Program. The samples were analyzed for about 170 water-quality constituents that included chlorofluorocarbons, physical properties, dissolved solids and major ions, nutrients and dissolved organic carbon, trace elements, pesticide compounds, and volatile organic compounds. The purpose of this report is to provide an assessment of water quality in recharge to shallow ground water underlying areas of recent residential and commercial development and to determine the relation of ground-water quality to overlying urban land use. Analyses of water from the 30 monitoring wells for chlorofluorocarbons were used to estimate apparent dates of recharge. Water from 18 wells with nondegraded and uncontaminated chlorofluorocarbon concentrations had calculated apparent recharge dates that ranged from 1979 to 1990 with an average date of 1986. Water from 14 monitoring wells (47 percent) exceeded the 500-milligrams-per-liter Secondary Maximum Contaminant Level established by the U.S. Environmental Protection Agency for dissolved solids in drinking water. The Secondary Maximum Contaminant Levels of 250 milligrams per liter for chloride and sulfate were exceeded in water from one well. The source of the largest concentrations of dissolved solids and associated ions, such as chloride and sulfate, in shallow ground water in the study area probably is highly mineralized water moving out of the Arkansas River into the adjacent, unconsolidated deposits and mixing with the dominant calcium bicarbonate water in the deposits. Concentrations of most nutrients in water from the sampled wells were small, with the exception of nitrate. Although water from the sampled wells did not have nitrate concentrations larger than the 10-milligram-per-liter Maximum Contaminant Level for drinking water, water from 50 percent of the sampled wells showed nitrate enrichment (concentrations greater than 2.0 milligrams per liter). Most trace elements in water from the sampled wells were detected only in small concentrations, and few exceeded respective water-quality standards. Twenty percent of iron concentrations, 40 percent of manganese concentrations, 3 percent of arsenic concentrations, and 13 percent of uranium concentrations exceeded respective Maximum Contaminant Levels or Secondary Maximum Contaminant Levels. A total of 47 pesticide compounds were analyzed in ground-water samples during this study. Water from 73 percent of the wells sampled had detectable concentrations of one or more of 8 of these 47 compounds. The herbicide atrazine or its degradation product deethylatrazine were detected most frequently (in water from 70 percent of the sampled wells). Metolachlor was detected in water from 10 percent of the wells, and simazine was detected in water from 30 percent of the wells sampled. Other pesticides detected included dieldrin, pendimethalin, prometon, and tebuthiuron (each in water from 3 percent of the wells). All concentrations of these compounds were less than established Maximum Contaminant Levels. A total of 85 volatile organic compounds (VOCs) were analyzed in ground-water samples during this study. Water from 43 percent of the wells had a detectable concentration of one or more VOCs. Chloroform was the most frequently detected VOC (23 percent of the wells sampled).Seven other VOCs were detected in water at frequencies of 13 percent or less in the wells sampled. Concentrations of VOCs were less than respective Maximum Contaminant Levels, except one sample with a concentration of 9.0 micrograms per liter for tetrachloroethylene (Maximum Contaminant Level of 5.0 micrograms per liter). An analysis of hydraulic gradient, flow velocity, and residence time of the ground water indicated potential recharge areas that ranged from 0.8 to 2.8 miles upgradient of monitoring-well locations. Nineteen (63 percent) of these potential recharge areas were in agricultural areas or areas in transition from agricultural to residential and (or) commercial land use at the time water sampled from the monitoring wells was recharged to the shallow ground water. The occurrence of atrazine or deethylatrazine in water from 70 percent of the monitoring wells may indicate a historical agricultural land-use relation. This agricultural relation also may affect concentrations of other water-quality constituents of possible agricultural origin such as nitrate, which generally were in excess of background concentrations in shallow ground water.

Kansas↗

Geologic and physiographic controls on bed-material yield, transport, and channel morphology for alluvial and bedrock rivers, western Oregon

The rivers of western Oregon have diverse forms and characteristics, with channel substrates ranging from continuous alluvial gravel to bare bedrock. Analysis of several measurable morphologic attributes of 24 valley reaches on 17 rivers provides a basis for comparing nonalluvial and alluvial channels. Key differences are that alluvial reaches have greater bar area, greater migration rates, and show systematic correlation among variables relating grain size to bed-material transport capacity. We relate these differences between channel types to bed-material transport rates as derived from a coupled regional analysis of empirical sediment yield measurements and physical experiments of clast attrition during transport. This sediment supply analysis shows that overall bed-material transport rates for western Oregon are chiefly controlled by (1) lithology and basin slope, which are the key factors for bed-material supply into the stream network, and (2) lithologic control of bed-material attrition from in-transport abrasion and disintegration. This bed-material comminution strongly affects bed-material transport in the study area, reducing transport rates by 50%–90% along the length of the larger rivers in the study area. A comparison of the bed-material transport estimates with the morphologic analyses shows that alluvial gravel-bed channels have systematic and bounding relations between bed-material transport rate and attributes such as bar area and local transport capacity. By contrast, few such relations are evident for nonalluvial rivers with bedrock or mixed-bed substrates, which are apparently more influenced by local controls on channel geometry and sediment supply. At the scale of western Oregon, the physiographic and lithologic controls on the balance between bed-material supply and transport capacity exert far-reaching influence on the distribution of alluvial and nonalluvial channels and their consequently distinctive morphologies and behaviors—differences germane for understanding river response to tectonics and environmental perturbations, as well as for implementing effective restoration and monitoring strategies.

California, Nevada, Oregon, Washington↗