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Biological water-quality assessment of selected streams in the Milwaukee Metropolitan Sewerage District Planning Area of Wisconsin, 2007

Changes in the water quality of stream ecosystems in an urban area may manifest in conspicuous ways, such as in murky or smelly streamwater, or in less conspicuous ways, such as fewer native or pollution-sensitive organisms. In 2004, and again in 2007, the U.S. Geological Survey sampled stream organisms—algae, invertebrates, and fish—in 14 Milwaukee area streams to assess water quality as part of the ongoing Milwaukee Metropolitan Sewerage District (MMSD) Corridor Study. In addition, passive-sampling devices (SPMDs, “semipermeable membrane devices”) were deployed at a subset of sites in order to evaluate the potential exposure of stream organisms to certain toxic chemicals. Results of the 2007 sampling effort are the focus of this report. Results of sampling from 2007 are compared with results from 2004. The water quality of sampled streams was assessed by evaluating biological-assemblage data, metrics computed from assemblage data, and an aggregate bioassessment ranking method that combined data for algae, invertebrates, and fish. These data contain information about the abundance (number) of different species in each group of stream organisms and the balance between species that can or cannot tolerate polluted or disturbed conditions. In 2007, the highest numbers of algal, invertebrate, and fish species were found at the Milwaukee River at Milwaukee, the largest sampled site. Algal results indicated water quality concerns at 10 of the 14 sampled sites due to the occurrence of nuisance algae or low percentages of pollution-sensitive algae. When compared to 2004, total algal biovolume was higher in 2007 at 12 of 14 sites, due mostly to more nuisance green algae from unknown causes. Results of several metrics, including the Hilsenhoff Biotic Index (HBI-10), suggest that invertebrate assemblages in the Little Menomonee River, Underwood Creek, and Honey Creek were poorer quality in 2007 compared to 2004. Six sites received “very poor” quality ratings for fish in 2007, mostly because inadequate numbers of fish were collected at five sites to allow computation of an Index of Biotic Integrity (IBI); this resulted in three additional sites receiving “very poor” ratings compared to 2004. Some signs of potential improvement in the fish assemblage were evident at Lincoln Creek, possibly reflecting delayed effects of the restoration of stream habitat, completed in 2002; however, algae and invertebrates did not show signs of improvement. Aggregate bioassessment rankings across all groups of organisms for 2004 and 2007 indicated that water quality at the two Milwaukee River main stem sites (at Milwaukee and near Cedarburg), Jewel Creek, and the Menomonee River at Menomonee Falls was the least-degraded among all sampled sites. Rankings for Oak Creek and Little Menomonee suggested water quality was worse in 2007 compared to 2004 and placed these two sites together with Kinnickinnic River and Underwood Creek, two concrete-line sites, indicating the most-degraded water quality among all sampled sites. The aggregate ranking for Lincoln Creek in 2007 would have placed it in the most-degraded category but for the positive influence of the fish ranking when compared to poor algal and invertebrate rankings. Potential toxicity due to certain manmade chemicals, such as polycyclic aromatic hydrocarbons (PAHs), was found at all six sites where SPMDs were deployed. As was found in 2004, the highest potential toxicity in 2007 was observed at Lincoln Creek where chemical screening in 2007 also showed the highest total PAHs of all six sites; however, potential toxicity at Little Menomonee River, Honey Creek, and Kinnickinnic River was relatively high compared to Milwaukee River near Cedarburg. Although toxicity and chemical results in 2007 did not agree with aggregate rankings for Lincoln Creek because of fish, nor for Honey Creek, the results did agree with aggregate rankings at four of the six sites. In addition to toxicological and chemical influences, the more urbanized sites have high percentages of impervious surface area, resulting in frequent high stream flows that can adversely affect algal, invertebrate, and fish assemblages. Assessments of the ecological status of different groups of organisms and of potential chemical and physical stressors to organisms are important tools in evaluating streamwater quality.

Wisconsin

Coastal habitats of the Elwha River, Washington- Biological and physical patterns and processes prior to dam removal

This report includes chapters that summarize the results of multidisciplinary studies to quantify and characterize the current (2011) status and baseline conditions of the lower Elwha River, its estuary, and the adjacent nearshore ecosystems prior to the historic removal of two long-standing dams that have strongly influenced river, estuary, and nearshore conditions. The studies were conducted as part of the U.S. Geological Survey Multi-disciplinary Coastal Habitats in Puget Sound (MD-CHIPS) project. Chapter 1 is the introductory chapter that provides background and a historical context for the Elwha River dam removal and ecosystem restoration project. In chapter 2, the volume and timing of sediment delivery to the estuary and nearshore are discussed, providing an overview of the sediment stored in the two reservoirs and the expected erosion mechanics of the reservoir sediment deposits after removal of the dams. Chapter 3 describes the geological background of the Olympic Peninsula and the geomorphology of the Elwha River and nearshore. Chapter 4 details a series of hydrological data collected by the MD-CHIPS Elwha project. These include groundwater monitoring, surface water-groundwater interactions in the estuary, an estimated surface-water budget to the estuary, and a series of temperature and salinity measurements. Chapter 5 details the work that has been completed in the nearshore, including the measurement of waves, tides, and currents; the development of a numerical hydrodynamic model; and a description of the freshwater plume entering the Strait of Juan de Fuca. Chapter 6 includes a characterization of the nearshore benthic substrate developed using sonar, which formed a habitat template used to design scuba surveys of the benthic biological communities. Chapter 7 describes the ecological studies conducted in the lower river and estuary and includes characterization of juvenile salmon diets and seasonal estuary utilization patterns using otolith analysis to determine habitat specific and hatchery compared with wild patterns in juvenile Chinook salmon, assessment of benthic and terrestrial macroinvertebrate communities, and seasonal patterns of water nutrients. In Chapter 8, the vegetation communities of the eastern estuary are characterized by mapped vegetation cover types and samples collected for vegetation composition and diversity. Chapter 9 summarizes the existing conditions of the study area as detailed in this report and describes some of the possible outcomes of river restoration on the coastal ecosystems of the Elwha River. Together, these different scientific perspectives form a basis for understanding the Elwha River ecosystem, an environment that has and will undergo substantial change. A century of change began with the start of dam construction in 1910; additional major change will result from dam removal scheduled to begin in September 2011. This report provides a scientific snapshot of the lower Elwha River, its estuary, and adjacent nearshore ecosystems prior to dam removal that can be used to evaluate the responses and dynamics of various system components following dam removal.

Scientific Investigations Report

Physical habitat, water quality, and riverine biological assemblages of selected reaches of the Sheyenne River, North Dakota, 2010

In 2010, data on physical habitat, water quality, and riverine biological assemblages were collected at selected reaches in four locations (Kleven, Sheyenne, Cooperstown, and West Fargo) on the Sheyenne River in east-central North Dakota. Three of the locations (Kleven, Sheyenne, and Cooperstown) are above Baldhill Dam and one location (West Fargo) is below Baldhill Dam on the Sheyenne River. The 2010 data provide information to establish a better understanding of the water-quality and ecological conditions of the Sheyenne River. Concerns were raised about the water-quality and ecological conditions of the Sheyenne River because of the interbasin transfer of water from nearby Devils Lake. The transfer of water from Devils Lake to the Sheyenne River occurs through the Devils Lake State Outlet near Peterson Coulee or, if lake elevations exceed 1,459 feet above National Geodetic Vertical Datum of 1929 (NGVD 29), through a natural outlet, Tolna Coulee. The field measurements of water-quality characteristics and results of chemical analyses generally are comparable to summary statistics calculated for Sheyenne River for 1980 through 2006. Overall, water-quality results show differences between the Kleven, Sheyenne, Cooperstown, and West Fargo reaches. Sulfate concentrations were less than the State of North Dakota criterion of 750 milligrams per liter for the upper Sheyenne River above Baldhill Dam and less than the criterion of 450 milligrams per liter for the lower Sheyenne River below Baldhill Dam. Arsenic concentrations at most reaches exceeded the U.S. Environmental Protection Agency drinking-water standard of 10 micrograms per liter. Nutrient concentrations (nitrogen, phosphorus) were higher in the upper Sheyenne River above Baldhill Dam than below Baldhill Dam where concentrations decreased by about half. In 2010, 35 families and 44 genera of benthic macroinvertebrates were collected and identified. On the basis of the index of biotic intergrity scores for benthic macroinvertebrate communities present in the Sheyenne River, all the reaches were determined to have condition classes of moderately disturbed to most disturbed. The benthic macroinvertebrate communities at the Cooperstown reaches were classed as moderately disturbed, whereas benthic macroinvertebrate communities at the Kleven, Sheyenne, West and Fargo reaches were most disturbed. During data collection, 37 genera and 165 species of periphyton (diatoms and soft-bodied algae) were collected and identified. In periphyton communities, similar taxa species were dominant in the Kleven, Sheyenne, and Cooperstown reaches, and different taxa species were dominant in the West Fargo reaches. For diatoms, the Kleven 3 reach had the lowest species richness value of 33.0, whereas the Cooperstown 8 reach had the highest species richness value of 57.0. For soft-bodied algae, the species richness values ranged from 8.0 at the Sheyenne 4 reach to 20.0 at the West Fargo 10 reach. During the fish collection, 32 species, representing 10 families, were collected in the Sheyenne River. All but two species are native to the Sheyenne River system. Common carp and white crappie are the two introduced species. Of the 32 species, 29 are tolerant to moderately tolerant to changes in water quality and habitat degradation, 16 species are tolerant to moderately tolerant to turbidity, and 16 species are tolerant to moderately tolerant to sensitivity to total dissolved solids, sulfate, and chloride. All fish species were categorized into four trophic groups. The largest group of 19 species was the insectivores (both benthic and general). The predator group consisted of seven species, and the omnivores consisted of six species. More fish were found in the lower Sheyenne River below Baldhill Dam than in the upper Sheyenne River above Baldhill Dam.

North Dakota

Biological and habitat assessment of the Lower Rouge River, Michigan 2018

A key component of evaluating the success of habitat remediation projects is determining preremediation conditions, biotic and abiotic, to establish a baseline and compare with postproject conditions. The Rouge River, Michigan, is a Great Lakes Area of Concern with a listed Beneficial Use Impairment related to loss of fish and wildlife habitat. A biological and habitat assessment was completed in the lower Rouge River, focused along a nearly 7-kilometer stretch of river that includes a concrete channel anticipated to be removed by 2022, to determine prerestoration conditions. Surveys documented the presence and quality of physical habitat, presence of herpetofauna, and quantified macroinvertebrate and fish assemblages at 12 sites (3 upstream from the concrete channel, 6 within the concrete channel, and 3 downstream from the concrete channel). Macroinvertebrate assemblages were dominated by Chironomidae and Oligochaeta for June and September. The electrofishing catch per unit effort was driven by Notropis atherinoides (emerald shiner) catches in June and emerald shiner and Dorosoma cepedianum (gizzard shad) catches in September. Graptemys geographica (northern map turtle) was the most common reptile observed throughout the lower Rouge River. No submergent macrophytes were discovered, and riparian vegetation was sparse in the concrete channel section. No sites scored “excellent” (total score greater than 154), upstream control sites scored “good” for overall qualitative habitat assessments (total score 105–154), and all concrete channel and downstream control sites were ranked as “marginal” (total score 56–104) or “poor” habitat (total score 0–55). Results from this assessment can be used to compare with postremediation projects in the lower Rouge River.

Michigan

Effects of stormwater runoff from selected bridge decks on conditions of water, sediment, and biological quality in receiving waters in South Carolina, 2013 to 2018

The U.S. Geological Survey, in cooperation with the South Carolina Department of Transportation, investigated the effects of stormwater runoff from bridge decks on stream water quality conditions in South Carolina. The investigation assessed 5 bridges in 3 physiographic provinces in South Carolina (Piedmont, Upper Coastal Plain, and Lower Coast Plain) that had a range of bridge, traffic, and hydrologic characteristics. The five selected South Carolina bridge sites (coincident with U.S. Geological Survey stations) and corresponding highways were Lynches River at Effingham (station 02132000; U.S. Highway 52), North Fork Edisto River at Orangeburg (station 02173500; U.S. Highway 301), Turkey Creek above Huger (station 02172035; South Carolina Highway 41), South Fork Edisto River near Denmark (station 02173000; U.S. Highway 321), and Fishing Creek at Highway 5 below York (station 021473415; South Carolina Highway 5). Bridge decks at the selected sites used open chutes, scuppers, and downspouts to drain stormwater directly into the receiving water at evenly spaced intervals. Stream water, sediment, and biological samples were collected and analyzed for a variety of constituents to evaluate the stream conditions for this study. Five to six stream samples were collected at transects upstream and downstream from each selected bridge site using the equal-width-increment technique during observable stormwater runoff. Routine samples of the receiving waters were collected 12 to 14 times at the upstream transect during nonstorm conditions. Samples were analyzed for physical properties, suspended sediment, nutrients, major ions, trace metals, polycyclic aromatic hydrocarbons, and Escherichia coli . Bridge-deck sediment and streambed sediment at upstream and downstream transects were collected once at each bridge site and analyzed for metals and semivolatile organic compounds that include polycyclic aromatic hydrocarbons. Benthic macroinvertebrate community surveys were conducted once using Hester-Dendy multiplate artificial substrate samplers deployed at multiple upstream and downstream transects concurrently. Statistical analysis of the water-quality data determined that stormwater runoff from bridges did not significantly degrade physical properties, nor nutrient, trace-metal, Escherichia coli , and suspended-sediment concentrations at the selected sites beyond the variability at the upstream transect (no bridge influence) during the study period. During storm sampling at the bridge sites, water-quality conditions were statistically similar upstream and downstream from each bridge, except for greater turbidity, total nitrogen, and total organic nitrogen plus ammonia concentrations found downstream from the bridge site on Fishing Creek; higher total chromium concentrations detected downstream from the bridge site on Turkey Creek; and increased Escherichia coli concentrations found downstream from the bridge site on the North Fork Edisto River. Total recoverable lead, cadmium, and copper concentrations were the only trace metals that periodically exceeded the South Carolina Department of Health and Environmental Control freshwater aquatic-life criteria at some bridge sites (lead, copper, and cadmium in Turkey Creek; cadmium and lead in Fishing Creek; lead in the South Fork Edisto River and Lynches River), but the exceedances occurred more frequently during routine sampling upstream from the bridge sites than during storm sampling at upstream and downstream transects. In general, stormwater runoff from the bridge decks did not seem to be the major source of metal enrichment in receiving waters during the study period. North Fork and South Fork Edisto Rivers and Turkey Creek had only one storm sample that exceeded South Carolina Department of Health and Environmental Control recreational criterion for Escherichia coli at both the upstream and downstream locations, while Fishing Creek had more frequent exceedances. Polycyclic aromatic hydrocarbons were detected infrequently in the stream samples. In general, sediment trace-metal concentrations were below the threshold and probable effect concentration at all bridge sites, except for the chromium concentration (45.1 milligrams per kilogram) detected upstream from the bridge site on Fishing Creek that exceeded the threshold effect concentration of 43.4 milligrams per kilogram. Based on enrichment ratios less than 1.5, bridge-deck runoff did not seem to be affecting trace-metal accumulation in the streambed sediment downstream from the bridge sites, except for lead at the bridge site on the Lynches River and manganese at the bridge site on Fishing Creek. Individual polycyclic aromatic compound concentrations and the sum of 18 compounds did not exceed any threshold and probable effect concentrations, indicating polycyclic aromatic hydrocarbon concentrations in the streambed sediment at downstream and upstream transects were not likely to affect the health of benthic macroinvertebrate communities. Although the cumulative polycyclic aromatic hydrocarbon concentrations in downstream sediment at the sites on Turkey and Fishing Creeks were well below the threshold effect concentration of 1,610 micrograms per kilogram, the 3- to 100-fold increase in downstream concentrations indicated a strong probability of a bridge-deck runoff source. Overall, benthic macroinvertebrate community health downstream from the bridge sites did not seem to be affected by bridge-deck runoff based on several multivariate analyses that indicated statistically similar benthic macroinvertebrate communities at upstream and downstream transects. Of the five bridge sites in this study, the site on Turkey Creek seemed to have the least healthy benthic macroinvertebrate communities because of the lowest Ephemeroptera, Plecoptera, and Trichoptera spp. (mayflies, stoneflies, and caddisflies, respectively) taxa, species richness, and diversity; and the highest biotic indices, indicative of poorer ecological health, at upstream and downstream transects. This ecological finding was not unexpected because of seasonal periods of negligible flow when dissolved-oxygen concentrations fell below 4 milligrams per liter during the study period. Of the five bridge sites in this study, the site on the South Fork Edisto River seemed to have healthier benthic macroinvertebrate communities because of the greater mean Ephemeroptera, Plecoptera, and Trichoptera spp. taxa; and lower mean biotic indices at upstream and downstream transects.

Scientific Investigations Report

Distribution and potential for adverse biological effects of inorganic elements and organic compounds in bottom sediment, lower Charles River, Massachusetts

Surficial-sediment samples and cores collected from the lower Charles River Basin are generally enriched in inorganic elements and organic compounds, including polychlorinated byphenyls, total organochlorine pesticides, and polyaromatic hydrocarbons. Median concentrations of selected inorganic elements and organic compounds measured in surficial-sediment samples collected from the lower Charles River ranged from 1. 3 to 3 5 times higher than median concentrations of these constituents measured in samples collected from urban rivers across the conterminous United States by the U.S. Geological Survey's National Water Quality Assessment Program (NAWQA). The distribution of the inorganic elements in surficial sediment throughout the basin appears to be controlled primarily by in-stream processes associated with sediment transport and the presence of an anoxic zone within a non-tidal salt wedge in the basin. In contrast, the distribution of organic compounds appears to reflect local point and non-point sources. Inorganic elements and organic compounds are present at sufficiently high concentrations at many surficial-sediment sampling sites to cause potentially severe biological effects to benthic organisms living in and on the bottom sediment. Ratios of acid volatile sulfide to simultaneously extracted metals, however, suggest that some inorganic elements would be toxic in fewer than 25 percent of the surficial-sediment samples. Many individual polyaromatic hydrocarbons, total petroleum hydrocarbons, and lead measured in cores exceeded exposure-based soil-concentration standards for direct contact and incident ingestion at areas where restoration of public wading and swimming beaches is being considered.

Massachusetts

Reconnaissance of chemical and biological quality in the Owyhee River from the Oregon State line to the Owyhee Reservoir, Oregon, 2001–02

The Owyhee River drains an extremely rugged and sparsely populated landscape in northern Nevada, southwestern Idaho, and eastern Oregon. Most of the segment between the Oregon State line and Lake Owyhee is part of the National Wild and Scenic Rivers System, and few water-quality data exist for evaluating environmental impacts. As a result, the U.S. Geological Survey, in cooperation with the Bureau of Land Management, assessed this river segment to characterize chemical and biological quality of the river, identify where designated beneficial uses are met and where changes in stream quality occur, and provide data needed to address activities related to environmental impact assessments and Total Maximum Daily Loads. Water-quality issues identified at one or more sites were water temperature, suspended sediment, dissolved oxygen, pH, nutrients, trace elements, fecal bacteria, benthic invertebrate communities, and periphyton communities. Generally, summer water temperatures routinely exceeded Oregon's maximum 7-day average criteria of 17.8 degrees Celsius. The presence of few coldwater taxa in benthic invertebrate communities supports this observation. Suspended-sediment concentrations during summer base flow were less than 10 milligrams per liter (mg/L). Dissolved solids concentrations ranged from 46 to 222 mg/L, were highest during base flow, and tended to increase in a downstream direction. Chemical compositions of water samples indicated that large proportions of upland-derived water extend to the lower reaches of the study area during spring runoff. Dissolved fluoride and arsenic concentrations were highest during base flow and may be a result of geothermal springs discharging to the river. No dissolved selenium was detected. Upstream from the Rome area, spring runoff concentrations of suspended sediment ranged from 0 to 52 mg/L, and all except at the Three Forks site were typically below 20 mg/L. Stream-bottom materials from the North Fork Owyhee River, an area with no mines, were enriched with nine trace elements, which indicates that this basin may be a natural source of these elements. Near Rome, the part of the study area not included in the National Wild and Scenic Rivers System, land-use impacts resulted in elevated populations of Escherichia coli bacteria ( E. coli ) during base flow and elevated concentrations of nitrogen and phosphorus during spring runoff. Sites in this area had the highest numbers of benthic invertebrates; the fewest Ephemeroptera, Plecoptera, and Trichoptera taxa; and the highest Hilsenhoff Biotic Index scores. These results suggest degraded stream quality. Periphyton communities at sites in this area approached nuisance levels and could cause significant dissolved oxygen depletions and pH values that exceed Oregon's recommended criteria. Stream-bottom materials from Jordan Creek were enriched with mercury and manganese, which probably were ultimately caused by past mining in that basin. Below Crooked Creek, elevated suspended sediment concentrations (142 mg/L), phosphorus concentrations (0.23 mg/L), and E. coli populations (370 most probable number per 100 milliliters) during the largest spring runoff event could be the result of inputs at the lower end of Jordan Valley and (or) inputs from Crooked Creek. The New Zealand Mud Snail, a highly competitive gastropod introduced to the Snake River in the 1980s, was collected just downstream from the Crooked Creek confluence.

Idaho;Nevada;Oregon

Physical, chemical, and biological relations of four ponds in the Hidden Water Creek strip-mine area, Powder River Basin, Wyoming

The Hidden Water Creek area in Wyoming was mined from 1944 to 1955 and abandoned. The open pits filled with water and pond-type ecosystems developed. Light was transmitted to greater depths within two control ponds located outside the mine area. The lower light transmittance in the ponds within the mined area probably was due, in part, to the greater number of phytoplankton cells. Also, unconsolidated soil material within the mine area was observed to slough off the pond banks, which could add to the concentration of suspended sediments. Dissolved oxygen concentrations were lower in the ponds within the mined area. Most of the major ions (calcium, magnesium, sulfate, and sodium) were present in greater concentrations in the ponds within the mined area. Higher concentrations of bicarbonate and total hardness were in the water within the mined area. Biological communities were less diverse and chemical concentrations fluctuated more in the mined area than in the ponds outside the mined area. (Woodard-USGS)

Water-Resources Investigations Report

Aquatic biology in Nederlo Creek, southwestern Wisconsin

This report presents the results of biologic investigations by the U.S. Geological Survey and the Wisconsin Department of Natural Resources during a study of hydrology and water quality in a small drainage basin in the "Driftless Area" of southwest Wisconsin. The investigation included aquatic macrophytes, periphytic and planktonic algae, benthic invertebrates, and trout population dynamics during 1967-78. The aquatic community is diverse and reasonably stable with little indication of environmental disturbance. Aquatic macrophyte population (dominated by Ranunculus aquatilis L., Veronica catenata Penn., and Nasturtium offlcinale) varies little from spring to fall. Periphytic and planktonic algae are predominantly diatoms, with the genus Achnanthes dominating both communities. Most genera of planktonic algae originate in the periphyton, but some true planktonic algae were identified. The benthic invertebrate population is dominated by Trichoptera and is a major food source for trout and forage fish. Biotic index values calculated from benthic invertebrate data indicate that water quality is very good to excellent. The trout population is low and represents only a small part of the total fish population both in biomass and numbers. Brown trout are usually stocked annually in the spring to enhance sport fishing, but by fall most trout are wild. The major environmental factors limiting trout population seem to be insufficient cover, insufficient pool depth and volume, and small spawning areas. The wild trout population is highly dependent on spawning success the previous fall.

Wisconsin

Physical, chemical, and biological characteristics of Pueblo Reservoir, Colorado, 1985-89

Physical, chemical, and biological characteristics of Pueblo Reservoir are described on the basis of data collected from spring 1985 through fall 1989. Also included are discussions of water quality of the upper Arkansas River Basin and the reservoir as they relate to reservoir operations. Pueblo Reservoir is a multipurpose, main-stem reservoir on the Arkansas River about 6 miles west of Pueblo, Colorado. At the top of its conservation pool, the reservoir is more than 9 miles long and ranges in depth from a few feet at the inflow to about 155 feet at the dam. Pueblo Reservoir derives most of its contents from the Arkansas River, which comprises native and transmountain flow. With respect to water temperature, the reservoir typically was well mixed to weakly stratified during the early spring and gradually became strongly stratified by May. The strong thermal stratification and underflow of the Arkansas River generally persisted into August, at which time the reservoir surface began to cool and the reservoir subsequently underwent fall turnover. Following fall turnover, the reservoir was stratified to some degree in the shallow upstream part and well mixed in the deeper middle and downstream parts. Reservoir residence times were affected by the extent of stratification present. When the reservoir was well mixed, residence times were as long as several months. During the summer when the reservoir was strongly stratified, reservoir releases were large, and when underflow was the prevalent flow pattern of the Arkansas River, reservoir residence times were as short as 30 days. Most particulate matter settled from the water column between the inflow and a distance of about 5 miles downstream. On occasions of large streamflows and sediment loads from the Arkansas River, particulate matter was transported completely through the reservoir. Water transparency, as measured with a Secchi disk, increased in a downstream direction from the reservoir inflow. The increase probably was a result of sediment settling from the water column in the upstream part of the reservoir. Secchi-disk depths in December through April were larger than those in May through November. Secchi-disk depths were small between May through August as inflow sediment loads and reservoir biomass increased. In the fall, Secchi-disk depths remained small possibly as the result of resuspension of sediment and detritus within the water column. Dissolved-oxygen concentrations generally were near supersaturation near the reservoir surface. Dissolved-oxygen concentrations decreased with increasing depth. On several occasions during the summer, dissolved oxygen became completely depleted in the hypolimnion of the downstream part of the reservoir. The most extensive period of anoxia that was measured was in August 1988; the bottom 12 to 30 feet of the downstream end of the reservoir was anoxic. Fall turnover typically resulted in well-oxygenated conditions throughout the water column from September or October through the spring. Values of pH ranged from 7.5 to 9.0 and typically were largest near the surface and decreased with depth. Dissolved-solids concentrations in the reservoir primarily are affected by dissolved solids in the inflow from the Arkansas River. Concentrations are largest during periods of decreased streamflows, September through April, and decrease with increasing streamflows in May through August. The median dissolved-solids concentration increased from 224 milligrams per liter at the inflow to 262 milligrams per liter at the outflow. However, a statistical analysis of dissolved solids indicated the apparent increase in dissolved-solids concentrations between the inflow and outflow was not significant. Calcium, sulfate, and bicarbonate are the major dissolved ions in Pueblo Reservoir. Concentrations of the major nutrients, nitrogen and phosphorus, varied within the reservoir because of settling of particulate matter, uptake by phytoplankton near the reservoir surface, and releases from the reservoir bottom sediments. Phosphorus was indicated to be a potentially growth-limiting nutrient in the reservoir because of its relatively small concentrations. During 1986 and 1987, the reservoir retained about 35 percent (359 tons) of the total nitrogen load and about 83 percent (203 tons) of the total phosphorus load. Settling of particulate matter from the water column and uptake by phytoplankton are the major nutrient sinks in the reservoir. Barium, iron, manganese, and zinc were the major trace elements in Pueblo Reservoir. Traceelement concentrations in the reservoir varied because of seasonality of trace-element concentrations in the Arkansas River, settling of particulate matter, and flux of trace elements from the bottom sediments. The aquatic-life standard in Pueblo Reservoir for total-recoverable iron (1,000 micrograms per liter) and the public water-supply standard for dissolved manganese (50 micrograms per liter) were exceeded on several occasions during the summer. Elevated concentrations of totalrecoverable iron and dissolved manganese in the Arkansas River during summer runoff contributed to exceedances in the upper part of the reservoir. Flux of manganese from the reservoir bottom sediments during periods of low or depleted dissolved-oxygen concentrations contributed to exceedances in the deeper, downstream parts of the reservoir. Concentrations of lead, mercury, and zinc were elevated in the reservoir bottom sediments and may be the result of metal-mine drainage in the upper Arkansas River Basin. Median concentrations of total organic carbon ranged from 3.1 to 4.5 milligrams per liter in May through September and from 2.5 to 3.5 milligrams per liter in October through April. Totalorganic-carbon concentrations in the reservoir were largest in the summer when streamflows and total-organic-carbon concentrations are largest in the Arkansas River. Total-organic-carbon concentrations in the reservoir decrease downstream from the reservoir inflow because of settling of particulate organic carbon. Levels of gross-alpha and gross-beta radioactivity generally were relatively low. In 7 of 31 samples collected, dissolved gross-alpha radioactivity, as natural uranium, exceeded 5 picocuries per liter, the level at which additional radiochemical analyses are recommended for drinking-water supplies. Potential sources of uranium in Pueblo Reservoir include weathering of exposed uranium ore deposits in the upper Arkansas River Basin and a uranium milling operation near Canon City. Phytoplankton densities and biovolumes measured during the winter, spring, and fall generally were indicative of a small to moderate algal biomass. Phytoplankton production tended to be largest during the summer. During the summer, phytoplankton densities and biovolumes generally were indicative of a moderate to large algal biomass. However, excessive algal production and biomass periodically occurred during the spring, summer, and fall. Three species of phytoplankton that are specifically associated with taste-and-odor problems in drinking water were identified on several occasions in water samples collected from Pueblo Reservoir. Reservoir operations and hydrodynamics can substantially affect processes that affect reservoir water quality. Stratification, underflow, and hypolimnetic withdrawals affect concentrations of dissolved solids, availability of nutrients, and concentrations of metals in the reservoir. Stratification impedes the mixing of epilimnetic and hypolimnetic waters, and the prevalent underflow that occurs during the summer results in a decrease in the potential dilution of inflowing river water with reservoir water. The underflow also decreases the maximum available nutrient load to the euphotic zone, which can, in turn, offset the maximum algal growth potential. Increased dissolved-solids, nutrient, and metal concentrations that occur in the hypolimnion during the summer are partially offset by hypolimnetic withdrawals.

Colorado

Nutrient loading and selected water-quality and biological characteristics of Dickinson Bayou near Houston, Texas, 1995-97

Data were collected at 10 stations in the Dickinson Bayou watershed near Houston, Texas, from March 1995 through February 1997 to estimate the concentrations, loads, and yields of selected nutrients that enter the bayou; to characterize the effects on nutrient concentrations of flow conditions, seasonality, and land use; and to identify nutrient sources (point or nonpoint) inferred from the occurrence and abundance of algal species in the benthic algal community. These data included rainfall samples, streamflow measurements, stream-water-quality samples, and biological samples, in addition to quality-assurance/quality-control samples. Estimates of loads of selected nutrients for the 106-square-mile watershed during the study were made for point sources and nonpoint sources. Point-source loading data are available only for ammonia nitrogen. Approximately 21.3 pounds per day of ammonia nitrogen is estimated from point sources during the study period. Nonpoint-source loads are estimated for eight nutrient forms: 7.84 pounds per day of dissolved ammonia nitrogen, 5.79 pounds per day of dissolved nitrite nitrogen, 215 pounds per day of dissolved Kjeldahl nitrogen, 350 pounds per day of total Kjeldahl nitrogen, 40.1 pounds per day of dissolved nitrite plus nitrate nitrogen, 67.6 pounds per day of total phosphorus, 46.6 pounds per day of dissolved phosphorus, and 42.8 pounds per day of dissolved orthophosphate. Rainfall-deposition rates also are estimated for comparison with point- and nonpoint-source loads. Deposition rates are 110 pounds per day of dissolved ammonia nitrogen, 120 pounds per day of dissolved nitrate nitrogen, and 15.8 pounds per day of dissolved phosphorus. Statistical tests were used to determine whether there are significant differences between nutrient concentrations during low-flow and during high-flow conditions. For basins with rural/mixed and urban land uses, nutrient concentrations generally are significantly different (greater) during storm events than during low flow, indicating accumulation in the watershed and subsequent washoff of nutrients. However, nutrient concentrations in storm-event samples consisting predominantly of runoff from a pasture are not significantly greater than those in low-flow samples. Statistical tests for seasonality indicate that dissolved ammonia nitrogen is significantly different in at least one season for all land uses (rural/residential, rural/mixed, and pasture) except urban. Concentrations tend to increase in the spring and early summer months, possibly from fertilizer application and subsequent washoff. Constituent-yield data were used to make direct comparisons of the nonpoint-source load contributions from four stations with watersheds of different land use. These comparisons lead to three conclusions: (1) For all nutrient species except orthophosphate, urban land use is the largest nonpoint-source contributor, (2) Kjeldahl nitrogen is the most abundant nutrient species, and (3) organic nitrogen accounts for the major part of the Kjeldahl nitrogen. Algal samples were collected at seven stations and were analyzed for periphyton identification and enumeration, and chlorophyll a and chlorophyll b concentrations. The large relative abundance of soil algae at stations in the middle of the watershed likely indicates the cumulative effects on water quality of agricultural nonpoint sources. Farther downstream near the State Highway 3 bridge, and downstream of three major tributary inflows, the increase in abundance of soil algae to a larger-than-expected level might reflect water-quality influences from predominantly urban nonpoint sources in the drainage basins of the three major tributary inflows. Nutrient concentrations do not appear to limit algal production in the upper (non-tidal) reach of Dickinson Bayou; but nutrient concentrations could have been limiting benthicalgal production in the lower (tidal) reach of the bayou during the time of the synoptic survey. If nitrogen is the limiting resource for algal productivity in the tidal reach of Dickinson Bayou, eutrophication of the system could be (at least partially) mitigated if nonpoint-source nutrient loads into the Bayou were reduced.

Texas

Characterization of selected biological, chemical, and physical conditions at fixed sites in the Upper Colorado River Basin, Colorado, 1995-98

Biological community samples were collected at 15 sites in the Upper Colorado River Basin (UCOL) in Colorado as part of the National Water-Quality Assessment (NAWQA) Program. Sites sampled in two physiographic provinces, the Southern Rocky Mountains and the Colorado Plateau, represented agriculture, mining, urban and recreation, and mixed land uses and background conditions. Nine measures of water quality, which include information on nutrients, specific conductance (a surrogate for salinity), trace elements in streambed sediment, pesticides in fish tissue, fish communities, and macroinvertebrate richness and composition and stream habitat were used for comparisons among sites within the two physiographic provinces. Sampling sites from three other NAWQA study units—the Rio Grande Valley, the South Platte River Basin, and the Upper Snake River Basin study units—were categorized on the basis of land use and stream size in order to develop a larger data set for comparison to sites in the UCOL. Three categories of land use—forested (includes mining, urban and recreation, and background), agriculture, and mixed—were used for comparison to the UCOL fixed sites. Results indicated that all sites other than the Colorado River below Baker Gulch (a background site) showed some water-quality characteristics to be significantly affected. Results indicated that the concentrations of cadmium and zinc in streambed sediment at mining land-use sites in the Southern Rocky Mountains physiographic province generally were orders of magnitude higher than streambed-sediment concentrations at the background site. Streambed-sediment concentrations at mining land-use sites in the UCOL were greater than the 75 th percentile of concentrations from sites in the three other NAWQA study units. Fish communities and habitat conditions were degraded at mining land-use sites compared to the background site. Ephemeroptera, Plecoptera, and Trichoptera (EPT) richness and the percentage of EPT were lower at mining land-use sites than at the background site and were less than the 50 th percentile of those for sites from the three other NAWQA study units. Nutrient concentrations at urban and recreation sites in the Southern Rocky Mountain physiographic province generally were greater than concentrations at the background site and generally were between the 25 th and 90 th percentile of concentrations for sites from the three other NAWQA study units. Habitat conditions and fish communities at urban and recreation sites were slightly degraded compared to the background site. EPT richness and the percentage of EPT were lower at urban and recreation sites than at the background site and were between the 25 th and 75 th percentile of those for sites from the three other NAWQA study units. The percentage of Chironomidae, which may be indicative of pollutant-tolerant organisms, was higher at urban and recreation sites than at the background site. Mixed land-use sites in the Southern Rocky Mountains physiographic province had similar nutrient concentrations and similar cadmium and zinc streambed-sediment concentrations. Fish-community degradation index values were very different among the three mixed land-use sites in the Southern Rocky Mountains physiographic province. Larger percentages of omnivores and anomalies such as lesions and deformities at two mixed land-use sites resulted in higher degradation values of the fish community. Agriculture land-use sites had higher concentrations of nutrients and selenium than the background site in the Colorado Plateau physiographic province. Concentrations of p,p '-DDE in fish tissue at agriculture sites were higher than the 75 th percentile of concentrations for sites from the three other NAWQA study units. Fish communities had degradation values near the 75 th percentile for agriculture sites. The percentage of EPT was low at agriculture sites when compared to the background site. Two mixed land-use sites in the Colorado Plateau physiographic province had similar concentrations of nutrients, selenium, and p,p '-DDE, and similar EPT richness and composition. These two sites were located downstream from agricultural and urban activities. Some water-quality measures at these two sites indicated degradation compared to a mixed land-use site upstream from most of the agriculture and urban activities in the Colorado Plateau physiographic province.

Colorado

Aquatic biology of the San Joaquin-Tulare basins, California; analysis of available data through 1992

Available data through 1992 on aquatic biota in the San Joaquin-Tulare Basins study unit of the National Water-Quality Assessment Program were analyzed to provide a conceptual framework to guide study design. The analysis included information on the biology of fish, aquatic macroinvertebrates, aquatic algae, and concentrations of trace elements and organic pesticides in aquatic biota.

Water Supply Paper

Step-changes in the physical, chemical and biological characteristics of the Gulf of Maine, as documented by the GNATS time series

We identify step-changes in the physical, chemical and biological characteristics of the Gulf of Maine (GoM) using the Gulf of Maine North Atlantic Time Series (GNATS), a series of oceanographic measurements obtained between September 1998 and December 2010 along a transect in the GoM running from Portland, ME, to Yarmouth, NS. GNATS sampled a period of extremes in precipitation and river discharge (4 of the 8 wettest years of the last century occurred between 2005 and 2010). Coincident with increased precipitation, we observed the following shifts: (1) decreased salinity and density within the surface waters of the western GoM; (2) both reduced temperature and vertical temperature gradients in the upper 50 m; (3) increased colored dissolved organic matter (CDOM) concentrations and particle scattering in the western GoM; (4) increased concentrations of nitrate and phosphate across all but the eastern GoM; (5) increased silicate, particularly in the western GoM, with a sharp increase in the ratio of silicate to dissolved inorganic nitrogen; (6) sharply decreased carbon fixation by phytoplankton; (7) moderately decreased chlorophyll, particulate organic carbon (POC) and particulate inorganic carbon (PIC) in the central GoM and (8) decreased POC- and PIC-specific growth rates. Gulf-wide anomaly analyses suggest that (1) the surface density changes were predominantly driven by temperature, (2) dissolved nutrients, as well as POC/PON, varied in Redfield ratios and (3) anomalies for salinity, density, CDOM, particle backscattering and silicate were significantly correlated with river discharge. Precipitation and river discharge appear to be playing a critical role in controlling the long-term productivity of the Gulf of Maine by supplying CDOM and detrital material, which ultimately competes with phytoplankton for light absorption.

Maine

Characterization of the biological, physical, and chemical properties of a toxic thin layer in a temperate marine system

The distribution of plankton in the ocean is patchy across a wide range of spatial and temporal scales. One type of oceanographic feature that exemplifies this patchiness is a ‘thin layer’. Thin layers are subsurface aggregations of plankton that range in vertical thickness from centimeters to a few meters, which may extend horizontally for kilometers and persist for days. We undertook a field campaign to characterize the biological, physical, and chemical properties of thin layers in Monterey Bay, California (USA), an area where these features can be persistent. The particle aggregates (marine snow) sampled in the study had several quantifiable properties indicating how the layer was formed and how its structure was maintained. Particles were more elongated above the layer, and then changed orientation angle and increased in size within the layer, suggesting passive accumulation of particles along a physical gradient. The shift in particle aggregate orientation angle near the pycnocline suggests that shear may also have played a role in generating the thin layer. Pseudo-nitzschia spp. were the most abundant phytoplankton within the thin layer. Further, both dissolved and particulate domoic acid were highest within the thin layer. We suggest that phosphate stress is responsible for the formation of Pseudo-nitzschia spp. aggregates. This stress together with increased nitrogen in the layer may lead to increased bloom toxicity in the subsurface blooms of Pseudo-nitzschia spp. Several zooplankton groups were observed to aggregate above and below the layer. With the knowledge that harmful algal bloom events can occur in subsurface thin layers, modified sampling methods to monitor for these hidden incubators could greatly improve the efficacy of early-warning systems designed to detect harmful algal blooms in coastal waters.

Marine Ecology Progress Series (MEPS)

Subcellular compartmentalization of Cd and Zn in two bivalves. I. Significance of metal-sensitive fractions (MSF) and biologically detoxified metal (BDM)

Many aspects of metal accumulation in aquatic invertebrates (i.e. toxicity, tolerance and trophic transfer) can be understood by examining the subcellular partitioning of accumulated metal. In this paper, we use a compartmentalization approach to interpret the significance of metal, species and size dependence in the subcellular partitioning of Cd and Zn in the bivalves Macoma balthica and Potamocorbula amurensis . Of special interest is the compartmentalization of metal as metal-sensitive fractions (MSF) (i.e. organelles and heat-sensitive proteins, termed Œenzymes¹ hereafter) and biologically detoxified metal (BDM) (i.e. metallothioneins [MT] and metal-rich granules [MRG]). Clams from San Francisco Bay, CA, were exposed for 14 d to seawater (20” salinity) containing 3.5 µg l -1 Cd and 20.5 µg l -1 Zn, including 109 Cd and 65 Zn as radiotracers. Uptake was followed by 21 d of depuration. The subcellular partitioning of metal within clams was examined following exposure and loss. P. amurensis accumulated ~22x more Cd and ~2x more Zn than M. balthica . MT played an important role in the storage of Cd in P. amurensis , while organelles were the major site of Zn accumulation. In M. balthica , Cd and Zn partitioned similarly, although the pathway of detoxification was metal-specific (MRG for Cd; MRG and MT for Zn). Upon loss, M. balthica depurated ~40% of Cd with Zn being retained; P. amurensis retained Cd and depurated Zn (~40%). During efflux, Cd and Zn concentrations in the MSF compartment of both clams declined with metal either being lost from the animal or being transferred to the BDM compartment. Subcellular compartmentalization was also size-dependent, with the importance of BDM increasing with clam size; MSF decreased accordingly. We hypothesized that progressive retention of metal as BDM (i.e. MRG) with age may lead to size dependency of metal concentrations often observed in some populations of M. balthica .

Marine Ecology Progress Series

Canada thistle biological control agents on two South Dakota wildlife refuges

We monitored populations of Canada thistle biocontrol agents Cassida rubiginosa, Ceutorhynchus litura, Larinus (= Hadroplantus) planus, Urophora cardui, Orellia (= Terellia) ruficauda, and Rhinocyllus conicus on Canada thistle (Cirsium arvense) at two national wildlife refuges in South Dakota from 1999 through 2003. C. litura, U. cardui, O. ruficauda, and R. conicus were present on both refuges. Agent populations were low except for C. litura, which was present in up to 90% of stems in some plots. C. litura infestation did not reduce thistle flowering, stem length, or over-winter survival. There was no change in thistle stem numbers over the study period and no difference in stem numbers in areas of high C. litura populations compared to areas of low C. litura populations. Our results suggest that insect biological control agents are inadequate for reduction of Canada thistle in southern South Dakota.

Natural Areas Journal

Components and predictors of biological soil crusts vary at the regional vs. plant community scales

Although biological soil crusts (biocrusts) occur globally in arid and semi-arid environments, most of our knowledge of biocrust cover and ecology is from a relatively small number of locations worldwide. Some plant communities are known to have high cover of biocrusts, but the abundance of biocrusts is largely undocumented in most plant communities. Using a data driven approach, we identified 16 plant communities based on plant cover from the Assessment, Inventory, and Monitoring Strategy data from the Bureau of Land Management (AIM, 5,200 plots). We found that abundance of lichens and mosses varies among communities, but that both components of biocrusts are present in all plant communities. Biocrusts are indicators of two of these communities: one that is defined by high cover of mosses and basin big sagebrush and one that is defined by high cover of lichens and shadscale saltbush. Using non-parametric multiplicative regression, we evaluated a suite of abiotic and disturbance variables to assess the degree to which climate and soils are associated with the abundance of lichens and mosses at the regional scale. At the regional scale, soil depth and maximum vapor pressure deficit were found to be strongly associated with the abundance of lichens and January minimum temperature dictated the abundance of mosses. At the scale of plant communities, community specific metrics of soils and climate were better able to explain the abundance of biocrusts. Our demonstration of the presence of biocrusts across the western US suggests that studies on ecosystem function could include these organisms because they are present in all plant communities, maintain arguably stronger associations with climatic variation, are directly associated with soils, and contribute to ecosystem functions that are not solely maintained by vascular plants.

Frontiers in Ecology and Evolution