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Influence of dietary zinc on cataracts in rainbow trout (Salmo gairdneri)

The purpose of this study was to determine the cause of lens cataracts in hatchery trout fed diets containing white fish meal. After preliminary investigations, three experiments were conducted with fry of rainbow trout ( Salmo gairdneri ) that were fed practical-type diets containing either 40% herring meal (control diet) or 40% white fish meal with and without various mineral supplements. In all experiments, the trout fed herring meal grew well and had normal lenses. Those fed the diet containing white fish meal without supplement grew slowly and developed bilateral cataracts determined by using a slit-lamp biomicroscope. The severity of cataracts was increased by supplementing the diet with a mixture of minerals (phosphates and carbonates of calcium, sodium and potassium). Cataracts were prevented, however, with supplemental Na2EDTA or zinc but not by supplements of manganese, copper, iron or various other minerals. The metabolic alterations responsible for the zinc-deficiency cataract were not determined.

Journal of Nutrition

Factors affecting dietary requirement and deficiency signs of L-tryptophan in rainbow trout

Two experiments were conducted to determine the concentration of dietary tryptophan needed for optimal growth and survival of fingerling rainbow trout ( Salmo gairdneri ), to characterize signs of tryptophan deficiency, and to ascertain the effects of niacin on deficiency signs. Test diets containing either hydrolyzed or intact casein were fed with graded levels of added L-tryptophan (0.0, 0.25, 0.50, 0.75 and 1.0 g/100 g protein). Maximum growth occurred at 0.75 and 0.50 g tryptophan in fish fed hydrolyzed and intact casein, respectively. Changes in mineral and amino acid mixtures in the intact casein diet yielded a cation-anion (Na + K - Cl) balance of + 15.3 meq/100 g diet and pH of 6.44, compared with a balance of -21.3 and -25 meq, and pH 5.8 and 4.4 for unmodified hydrolyzed and intact casein diets, respectively. Analysis of growth by the polynomial regression function; Y = 0.16 + 0.429 X - 0.26 X 2 , where Y = mean daily gain in grams, and X = grams of added tryptophan/100 g protein, predicted that at least 0.58 g of tryptophan was needed for maximum weight gain. Analysis of apparent pathology-free fish by the function; Y = 34.33 + 147.33 X = 83.87 X 2 , where Y = percentage with no signs of deficiency, and X = grams of added tryptophan, predicted that 0.63 g tryptophan/100 g protein was needed for optimum health scores. Deletion of supplemental niacin did not affect fish response to tryptophan.

Journal of Nutrition

Effect of excess dietary riboflavin on growth of rainbow trout

Fingerling rainbow trout were fed semipurified diets containing graded levels of supplemental riboflavin (3, 35, 42, 49, 56, 63, 70, 100 and 600 mg/kg of diet) to determine if dietary riboflavin in excess of requirements decreases growth. In three trials with three sizes of fish (mean initial weights, 0.5, 3.2 and 4.3 g) and two water temperatures (8.3° and 15°C), no significant diet-related growth inhibition was detected. The results suggest that rainbow trout, like other animals, are insensitive to excesses of dietary riboflavin and that the growth depression reported by other workers was the result of some other dietary influence or of faulty experimental design.

Journal of Nutrition

Storage by bobwhite quail of vitamin A fed in various forms

According to studies conducted with 236 bobwhite quail chicks at Patuxent Research Refuge, crystalline carotene in cottonseed oil fed at levels of 3000 I.U. (the requirement for optimum growth), 5000 I.U., and 25,000 I.U. per pound of feed, was utilized only ⅓ to 1/7 as efficiently as vitamin A alcohol; ½; to 1/10 as natural vitamin A ester; and ¼ to 1/17 as vitamin A acetate, based on the storage of vitamin A in the liver. The carotene in the natural carrier, alfalfa leaf meal, was assimilated as effectively as was crystalline carotene in cottonseed oil, when both were fed at 5000 I.U. per pound of feed. Crystalline carotene when fed at a level over 8 times the requirement of the quail for maximum growth, or 25,000 I.U. per pound of feed, was stored as vitamin A in the livers of pen-reared quail to an extent comparable to the levels found in certain young wild quail. Vitamin A acetate was utilized more efficiently by quail than either vitamin A alcohol or the natural vitamin A ester. There was considerable individual variation in storage of vitamin A by quail on the same diet, and with the same or similar parental background.

Journal of Nutrition

Calcium and phosphorus requirements of breeding bobwhite quail

In the course of studies designed to determine the calcium and phosphorus requirements of breeding bobwhite quail, it was found that best results were obtained when the Ca/P ratio in the diet was approximately 2.3:1. Variations in the Ca/P ratio produced significant differences in results when the level of phosphorus in the diet was 0.75%, but the differences were less marked when the level of phosphorus was increased to 1.00%. Although diets containing 0.75% phosphorus and 1.8% calcium appeared adequate for reproduction, as judged by the criteria of the maintenance of satisfactory condition in the breeders, egg production, fertility, hatchability and survival of offspring during the first 5 days after hatching, it was found that the winter mortality of the offspring of birds fed such a diet was much greater than that occurring in the offspring of birds fed on diets containing 1.00 or 1.25% phosphorus. It is concluded that breeding bobwhite quail require diets furnishing approximately 1.00% phosphorus and 2.3% calcium.

Journal of Nutrition

Dietary bioprocessed soybean meal does not affect the growth of exercised juvenile rainbow trout (Oncorhynchus mykiss)

Context : This 88-day experiment evaluated the rearing performance of juvenile rainbow trout ( Oncorhynchus mykiss ) fed one of three isonitrogenous and isocaloric diets and reared at velocities of either 2.3 or 18.7 cm s -1 . Objective : Evaluate the effects of diet and exercise during rainbow trout rearing. Design : Fishmeal was the primary protein source for one diet, with bioprocessed soybean meal (BSM) replacing either 60 or 85% of the fishmeal in the other two diets. Setting : This study was performed at Cleghorn Springs State Fish Hatchery in Rapid City, South Dakota, USA. Results : At the end of the experiment there were no significant differences among the dietary treatments in gain, percent gain, specific growth rate (SGR), or percent mortality. However, fish fed the fishmeal-based diet ate significantly more, experienced a significantly higher feed conversion ratio (FCR), and had a significantly higher hepatosomatic index than the fish fed the 85% BSM diet. Intestinal histology was not affected by the inclusion of BSM. Fish reared at 2.3 cm/s -1 had significantly lower FCRs, gain, percent gain, and SGR than the fish reared at 18.7 cm/ s -1 . There was a significant interaction in food consumed between diet and velocity, but no other significant interactions between the dietary and exercise treatments were observed. Conclusion : Based on these results, BSM can replace at least 85% of the fishmeal in juvenile rainbow trout, even if the fish are exercised.

South Dakota

Impact of fishing and stocking practices on Coregonid diversity

Fish species diversity can be lost through interacting stressors including habitat loss, stocking and overfishing. Although a multitude of stressors have played a role in the global decline of coregonid (Coregonus spp.) diversity, a number of contemporary studies have identified habitat loss stemming from eutrophication as the primary cause. Unfortunately, reconstructing the role of fishing and stocking practices can be difficult, because these records are incomplete or appear only in hard-to-access historic grey literature. Based on an illustrative set of historic and contemporary studies, we describe how fisheries management practices may have contributed to coregonid diversity loss in European and North American lakes. We provide case studies examining how fishing and stocking may reduce coregonid diversity through demographic decline and introgressive hybridization. In some lakes, fisheries management practices may have led to a loss of coregonid diversity well before issues with habitat degradation manifested. Our review suggests that fish conservation policies could beneficially consider the relative importance of all stressors, including management practices, as potential drivers of diversity loss.

Great Lakes, Lake Constance, Lake Geneva, Lake Gre