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Simulation of groundwater flow and interaction of groundwater and surface water on the Lac du Flambeau Reservation, Wisconsin

The Lac du Flambeau Band of Lake Superior Chippewa and Indian Health Service are interested in improving the understanding of groundwater flow and groundwater/surface-water interaction on the Lac du Flambeau Reservation (Reservation) in southwest Vilas County and southeast Iron County, Wisconsin, with particular interest in an understanding of the potential for contamination of groundwater supply wells and the fate of wastewater that is infiltrated from treatment lagoons on the Reservation. This report describes the construction, calibration, and application of a regional groundwater flow model used to simulate the shallow groundwater flow system of the Reservation and water-quality results for groundwater and surface-water samples collected near a system of waste-water-treatment lagoons. Groundwater flows through a permeable glacial aquifer that ranges in thickness from 60 to more than 200 feet (ft). Seepage and drainage lakes are common in the area and influence groundwater flow patterns on the Reservation. A two-dimensional, steady-state analytic element groundwater flow model was constructed using the program GFLOW. The model was calibrated by matching target water levels and stream base flows through the use of the parameter-estimation program, PEST. Simulated results illustrate that groundwater flow within most of the Reservation is toward the Bear River and the chain of lakes that feed the Bear River. Results of analyses of groundwater and surface-water samples collected downgradient from the wastewater infiltration lagoons show elevated levels of ammonia and dissolved phosphorus. In addition, wastewater indicator chemicals detected in three downgradient wells and a small downgradient stream indicate that infiltrated wastewater is moving southwest of the lagoons toward Moss Lake. Potential effects of extended wet and dry periods (within historical ranges) were evaluated by adjusting precipitation and groundwater recharge in the model and comparing the resulting simulated lake stage and water budgets to stages and water budgets from the calibrated model. Simulated lake water budgets and water level changes illustrate the importance of understanding the position of a lake within the hydrologic system (headwater or downstream), the type of lake (surface-water drainage or seepage lake), and the role of groundwater in dampening the effects of large-scale changes in weather patterns on lake levels. Areas contributing recharge to drinking-water supply wells on the Reservation were delineated using forward particle tracking from the water table to the well. Monte Carlo uncertainty analyses were used to produce maps showing the probability of groundwater capture for areas around each well nest. At the Main Pumphouse site near the Village of Lac du Flambeau, most of the area contributing recharge to the wells occurs downgradient from a large wetland between the wells and the wastewater infiltration lagoons. Nonetheless, a small potential for the wells to capture infiltrated wastewater is apparent when considering uncertainty in the model parameter values. At the West Pumphouse wells south of Flambeau Lake, most of the area contributing recharge is between the wells and Tippecanoe Lake. The extent of infiltrated wastewater from two infiltration lagoons was tracked using the groundwater flow model and Monte Carlo uncertainty analyses. Wastewater infiltrated from the lagoons flows predominantly south toward Moss Lake as it integrates with the regional groundwater flow system. The wastewater-plume-extent simulations support the area-contributing-recharge simulations, indicating that there is a possibility, albeit at low probability, that some wastewater could be captured by water-supply wells. Comparison of simulated water-table contours indicate that the lagoons may mound the water table approximately 4 ft, with diminishing levels of mounding outward from the lagoons. Four scenarios, representing potential alternatives for wastewater management, were simulated (at current discharge rates) to evaluate the potential extent of wastewater in the aquifer and discharge to surface-water bodies associated with each management scenario. Wastewater simulated to infiltrate through a hypothetical diffuser below a wetland south of the current lagoons appears to discharge to the overlying wetland and would likely discharge to Moss Lake as overland flow. Wastewater simulated to discharge to a small lake (Mindy Lake) between Moss and Fence Lakes appears to spread radically over a large area between the lakes. Wastewater simulated to discharge to lagoons south and northeast of the current lagoons also appears to spread radially, but the areas of the aquifer with the highest probability of encountering waste-water contamination would likely be between the lagoons and the nearest lake, where the wastewater would eventually discharge. Probability results for the wastewater-plume-extent scenarios are sensitive to the number of mathematical water particles used to represent infiltrating wastewater and the level of detail in the synthetic grid used for the probability analysis. Thus, probability results from wastewater-plume-extent simulations are qualitative only; however, it is expected that illustrations of relatively high or low probability will be useful as a general guide for decision making. Management problems requiring quantitative estimates of probability are best re-cast into problems evaluating the area that contributes recharge to the location of interest, which is not dependent upon the number of simulated particles or the resolution of a synthetic grid.

Wisconsin

Guadalupe Bass flow-ecology relationships; with emphasis on the impact of flow on recruitment

Guadalupe Bass Micropterus treculii is an economically and ecologically important black bass species endemic to the Edwards Plateau ecoregion and the lower portions of the Colorado River in central Texas. It is considered a fluvial specialist and as such, there are concerns that the increasing demands being placed upon the water resources of central Texas by growing human populations have the potential to negatively impact Guadalupe Bass populations. Therefore, this study assessed the relationship between Guadalupe Bass growth, feeding ecology, and streamflow. Sagittal otoliths were removed from Guadalupe Bass collected from throughout their range during 2015-2017 and used to estimate the age and back-calculate the growth trajectory of each individual. Additionally, young-of-year (YOY) Guadalupe Bass were collected every 10-14 days from two second-order streams, the North Llano River and South Llano River, in the Colorado Basin on the Edwards Plateau. Stomach contents of these individuals were identified and the effect of streamflow on the occurrence of the taxa comprising the stomach contents assessed. Guadalupe Bass growth was greater in the Colorado and Guadalupe River basins, independent of stream order, and tended to increase with increasing stream order within a basin. Growth was higher in higher stream orders and during years with stable and lower spring and summer monthly median flows, lower minimum and maximum flows, slower rise and fall rates, and higher baseflows. Growth was not influenced by years with higher monthly median flows in winter. These results would seem to contradict previous research, but more likely represent a fuller picture of how Guadalupe Bass respond to flow conditions. The disagreement between the current study and past studies seem to be attributable, at least in part, to the fact that previous studies were conducted during a period of extensive drought, while the current study was conducted during relatively wet conditions. Taken together with previous studies, the current study suggests that Guadalupe Bass growth is sensitive to flow conditions and is lower in years with flow conditions that fall outside a basin- and stream order-specific optimal range for the species. A total of 21 unique taxonomic groups were recovered from the stomachs of YOY Guadalupe Bass collected from the North Llano River and South Llano River. Aquatic insects, especially larval mayflies (Ephemeroptera), damselflies (Odanata: Zygoptera), and caddisflies (Trichoptera), were the most frequently encountered taxa. While there was no difference between the two rivers in stomach content composition, there was a strong longitudinal gradient in both systems with aquatic insects predominating at upstream sample sites and fishes being more common at downstream sites. Stream discharge during the 24 hours prior to collection did not have any influence on the probability of a taxa being found in Guadalupe Bass stomachs. The results of this study support efforts to manage Guadalupe Bass populations at a sub-watershed scale and suggests that populations occupying the same stream order within a basin are likely to have similar responses to annual flow conditions. In addition, these results indicate that the lower Colorado River population may inhabit a unique set of conditions that has supported the development of a trophy Guadalupe Bass fishery. Further, this study highlights the need to incorporate a sufficient range of annual flow conditions to ensure that the influence of stream flow on fish growth is adequately assessed. While interannual variation in growth rates seem to be capable of serving as a proxy for recruitment and year-class strength, long-term monitoring of recruitment paired with assessment of growth is necessary to further clarify the relationship between population density, flow regime, recruitment and growth and allow the construction of predictive models.

Texas

Influences of seasonality and habitat quality on Great Lakes coastal wetland fish community composition and diets

Great Lakes coastal wetlands (GLCW) have been severely degraded by anthropogenic activity over the last several decades despite their critical role in fish production. Many Great Lakes fish species use coastal wetland habitats for spawning, feeding, shelter, and nurseries throughout the year. The goal of our study was to compare GLCW fish community composition in the spring, summer, and fall months and investigate how water quality relates to fish diversity, the presence of functional groups, and juvenile fish diets. We summarized fish data collected from GLCW across the basin and used the coastal wetland monitoring program’s water quality-land use indicator to quantify water quality. Basin-wide, we found taxonomic and functional group differences in community composition among three sampling seasons, as well as across the range of water quality. Water quality was positively associated with the abundance of small cyprinids and the relative abundance of some habitat and reproductive specialists. Seasonal differences were also observed for many of these functional groups, with more temperature- and pollution-sensitive fishes captured in the spring and more nest-spawning fishes captured in the summer and fall. In our diet study, we found that age-0 fish primarily consumed zooplankton in the fall, whereas age-1 fish primarily consumed macroinvertebrates in the spring. Moreover, wetland quality was positively associated with trichopteran prey abundance. We concluded that taxonomic and functional composition of fish communities in GLCW vary markedly with respect to water quality and season. Thus, a full understanding of communities across a gradient of quality requires multi-season sampling.

Great Lakes

Herbivory on shoalgrass by wintering redheads in Texas

An estimated 80% of redheads ( Aythya americana ) winter on the Laguna Madre of south Texas and Mexico and feed almost exclusively on shoalgrass ( Halodule wrightii ) rhizomes. Shoalgrass abundance has decreased by 60% over the past 30 years, and because the effects of shoalgrass loss on wintering redheads are unknown, we initiated a study to define habitat selection criteria and document the effect of wintering redheads on shoalgrass in the lower Laguna Madre, Texas. Redheads consumed an average of 75% of shoalgrass rhizome biomass at collection sites each winter. When rhizome biomass was grazed to a mean biomass of ltoreq 0.18 g dry mass/core (approximately 10 g dry mass/ml), shoalgrass did not recover to its previous level the following growing season. Thirty-three percent of the sites (10) were grazed below 0.18 g dry mass/core during both years of the study, while 64% (19) were grazed below 0.18 g during 1 or the other of the 2 winters. Ramet number was positively correlated ( P < 0.001, r 2 = 0.54) with rhizome biomass; however, this relationship was influenced by grazing intensity. Heavy grazing reduced the amount of rhizome attached to each ramet compared with ungrazed ramets. Grazing had no effect on root biomass ( P = 0.388), rhizome moisture content ( P = 0.553), or soil magnesium, phosphorous, and potassium ( P = 0.102, 0.499, 0.162, respectively). Redhead presence increased ( P = 0.042) soil nitrogen levels. Foraging areas selected by redheads within the lower Laguna Madre had lower ( P = 0.026) salinities (24 ppt) than areas not selected (35 ppt). Redheads did not select foraging areas in relation to crude protein levels in rhizomes. Shoalgrass habitat in the Laguna Madre should be protected from further losses and enhanced where possible.

Texas

Explaining differences between bioaccumulation measurements in laboratory and field data through use of a probabilistic modeling approach

In the regulatory context, bioaccumulation assessment is often hampered by substantial data uncertainty as well as by the poorly understood differences often observed between results from laboratory and field bioaccumulation studies. Bioaccumulation is a complex, multifaceted process, which calls for accurate error analysis. Yet, attempts to quantify and compare propagation of error in bioaccumulation metrics across species and chemicals are rare. Here, we quantitatively assessed the combined influence of physicochemical, physiological, ecological, and environmental parameters known to affect bioaccumulation for 4 species and 2 chemicals, to assess whether uncertainty in these factors can explain the observed differences among laboratory and field studies. The organisms evaluated in simulations including mayfly larvae, deposit-feeding polychaetes, yellow perch, and little owl represented a range of ecological conditions and biotransformation capacity. The chemicals, pyrene and the polychlorinated biphenyl congener PCB-153, represented medium and highly hydrophobic chemicals with different susceptibilities to biotransformation. An existing state of the art probabilistic bioaccumulation model was improved by accounting for bioavailability and absorption efficiency limitations, due to the presence of black carbon in sediment, and was used for probabilistic modeling of variability and propagation of error. Results showed that at lower trophic levels (mayfly and polychaete), variability in bioaccumulation was mainly driven by sediment exposure, sediment composition and chemical partitioning to sediment components, which was in turn dominated by the influence of black carbon. At higher trophic levels (yellow perch and the little owl), food web structure (i.e., diet composition and abundance) and chemical concentration in the diet became more important particularly for the most persistent compound, PCB-153. These results suggest that variation in bioaccumulation assessment is reduced most by improved identification of food sources as well as by accounting for the chemical bioavailability in food components. Improvements in the accuracy of aqueous exposure appear to be less relevant when applied to moderate to highly hydrophobic compounds, because this route contributes only marginally to total uptake. The determination of chemical bioavailability and the increase in understanding and qualifying the role of sediment components (black carbon, labile organic matter, and the like) on chemical absorption efficiencies has been identified as a key next steps.

Integrated Environmental Assessment and Management

Influence of basin- and local-scale environmental conditions on nearshore production in the northeast Pacific Ocean

Nearshore marine habitats are productive and vulnerable owing to their connections to pelagic and terrestrial landscapes. To understand how ocean basin- and local-scale conditions may influence nearshore species, we developed an annual index of nearshore production (spanning the period 1972–2010) from growth increments recorded in otoliths of representative pelagic-feeding (Black Rockfish Sebastes melanops ) and benthic-feeding (Kelp Greenling Hexagrammos decagrammus ) nearshore-resident fishes at nine sites in the California Current and Alaska Coastal Current systems. We explored the influence of basin- and local-scale conditions across all seasons at lags of up to 2 years to represent changes in prey quantity (1- or 2-year time lags) and quality (within-year relationships). Relationships linking fish growth to basin-scale (Pacific Decadal Oscillation, North Pacific Gyre Oscillation, and multivariate El Niño–Southern Oscillation index) and local-scale (sea surface temperature, sea surface height anomalies, upwelling index, photosynthetically active radiation, and freshwater discharge) environmental conditions varied by species and current system. Growth of Black Rockfish increased with cool basin-scale conditions in the California Current and warm local-scale conditions in the Alaska Coastal Current, consistent with existing hypotheses linking climate to pelagic production on continental shelves in the respective regions. Relationships for Kelp Greenlings in the California Current were complex, with faster growth related to within-year warm conditions and lagged-year cool conditions. These opposing, lag-dependent relationships may reflect differences in conditions that promote quantity versus quality of benthic invertebrate prey in the California Current. Thus, we hypothesize that benthic production is maximized by alternating cool and warm years, as benthic invertebrate recruitment is food limited during warm years while growth is temperature limited by cool years in the California Current. On the other hand, Kelp Greenlings grew faster during and subsequent to warm conditions at basin and local scales in the Alaska Coastal Current.

Marine and Coastal Fisheries: Dynamics, Management

Feeding habits of sympatric aoudad (Ammotragus lervia) and desert bighorn sheep (Ovis canadensis mexicana) in West Texas

Aoudad ( Ammotragus lervia ), native to northern Africa, were introduced as exotic game animals to the Chihuahuan Desert in West Texas, USA, and have become invasive. Aoudad and bighorn sheep ( Ovis canadensis mexicana ) are adapted to rugged terrain in arid climates, and both persist in desert regions with low primary productivity and limited perennial water availability, which suggests potential for competition for food and water resources. Aoudad are highly adaptable, which could make them more resilient to a changing environment with extreme conditions, providing a competitive edge over bighorn sheep. To evaluate the potential for exploitative competition between invasive aoudad and endemic desert bighorn sheep, we used genetic metabarcoding to assess diet composition using fecal samples collected from adults of each species in the Sierra Vieja Mountains in West Texas. We collected 32 composite samples from aoudad and 27 composite samples for bighorn sheep and identified 88 genera consumed. Bighorn sheep and aoudad diets (as inferred by genera) were most different during the warm-wet season (16 June–15 October; Kulczynski similarity index = 0.81) and most similar during the warm-dry season (16 February–15 June; Kulczynski similarity index = 1.05). During the warm-wet season, the 2 herbivores tended to consume different genera, suggesting the possibility of resource partitioning, with less likelihood of resource partitioning during the warm-dry season when forage was more limited and diets were similar. Diet diversity, measured by Shannon's diversity index, did not vary substantially between species, but for aoudad it was highest during the warm-wet season (1.1 ± 0.0.1 [SE]) and lowest during the cool-dry season (16 October–15 February, 0.9 ± 0.0.1). For bighorn sheep, diet richness was highest during the cool-dry season (1.1 ± 0.2) and lowest during the warm-dry season (0.8 ± 0.10). Bighorn sheep may specialize on high-quality forage species, particularly during the warm-wet season, while aoudad have a more generalist foraging strategy, although temporal windows for increased competition for food resources likely occur. Aoudad are well-adapted to low-quality forage and arid climates, which could increase their competitive ability and compromise bighorn sheep conservation efforts in areas of sympatry.

Texas

Quantifying sea otter abundance, distribution, habitat use, and foraging intake in Cook Inlet, Alaska

Following near extirpation from the fur trade, sea otters ( Enhydra lutris ) have returned to occupy lower Cook Inlet since the 1950s, or earlier, with numbers increasing to ~11,000 and ~9,000 on the west and east side, respectively, by 2017. Northward range expansion on the west side has been negligible for decades with few animals found north of Kamishak Bay, while northward expansion on the east side has been more pronounced in recent decades. The reasons for these contrasting distribution patterns are not certain. Possible explanations for lack of expansion on the west side included 1) poor sea otter habitat north of Kamishak Bay; 2) adequate habitat north of Kamishak Bay but no incentive for sea otters to move north because of abundant food in Kamishak Bay, and/or sea otters discouraged from moving north of Kamishak Bay; 3) seasonal ice formation; or 4) seasonal presence of killer whales. This project was designed to document current sea otter abundance and distribution in lower Cook Inlet, including seasonal variation, and evaluate drivers of habitat use and foraging conditions, including how these overlap with Bureau of Ocean Energy Management (BOEM) Lease Sale blocks. We found that the probability of sea otter presence in lower Cook Inlet was significantly related to depth and distance from shore with the highest probabilities of sea otter presence in areas ~7–8 kilometers (km) from shore in water ~20–30 meters (m) deep. Historical survey data suggest sea otter distribution has not changed dramatically since 2002, and while we detected significant seasonal changes including due to presence of heavy sea ice, the seasonal changes were modest and primarily related to locations with consistent winter ice formation on the west side of lower Cook Inlet. Overall, throughout the year, sea otters appeared to be utilizing most of the habitat within the 40-m depth contour on the east side of lower Cook Inlet, including Kachemak Bay. Sea otters on the west side reside largely within Kamishak Bay. Because of the shallow bathymetry of lower Cook Inlet, sea otters consistently occurred farther offshore than in many other areas of Alaska. The presence of sea otters, including females with pups, in these offshore waters indicates that sea otters can forage productively in these open water areas. Importantly, we documented that sea otters, including females with pups, occurred in most of the eastern BOEM lease blocks including the original Lease Sale 244 blocks 7064, 7114, 6162, 6310, 6360, 6410, 6458 and 6457. The relinquishments for these 7 leases were effective September 17, 2024: OCS-Y-02434 (block # 7064), OCS-Y-02435 (7114), OCS-Y-02436 (6162), OCS-Y-02438 (6357), OCS-Y-02442 (6407), OCS-Y02446 (6457), OCS-Y-02447 (6458) https://www.boem.gov/sites/default/files/documents/environment/Map%20of%20Active%20Leases%20 Cook%20Inlet%20OCS_0.pdf Regarding differential northward expansion on the east and west side of lower Cook Inlet, we found that wind and water circulation patterns make winter sea ice more prominent on the west side, but this had minimal effects on sea otter distribution. In addition, although there have been observations of killer whale ( Orcinus orca ) predation on sea otters in Cook Inlet, we did not see behavioral or distributional evidence that it was prevalent enough to have strong effects on sea otter habitat use. Benthic surveys using a remotely operated vehicle (ROV) indicated that epibenthic substrate and biological community heterogeneity differed between areas with and without otters, suggesting that these factors may explain the current distribution of otters within lower Cook Inlet. However, ROV surveys cannot assess infaunal prey abundance, making assessments of the role of prey availability difficult. The existence of a healthy Pacific razor clam ( Siliqua patula ) fishery along western lower Cook Inlet along the Lake Clark coast north of Kamishak Bay suggests there may still be a resource base for eventual expansion of sea otters into this area. Our shore-based forage observations indicated that sea otters exist near carrying capacity densities relative to nearshore prey resources. However, our distribution model suggests the bulk of the population lives offshore beyond our ability to observe their feeding activity. The fact that sea otters are a consistent presence in offshore areas suggests that prey resources in these areas were relatively abundant in comparison to nearshore prey resources, making foraging in offshore areas, with water depths of 0–40 m, energetically profitable. Prey types in these offshore areas of lower Cook Inlet likely included epifauna such as crabs and large urchins, in addition to infaunal clams based on the soft substrate habitat types that characterize lower Cook Inlet. Collectively, our results suggest that sea otters occupy most areas of lower Cook Inlet with appropriate benthic habitat types and prey resources, which includes areas within BOEM Lease Sale blocks. With the exception of the Lake Clark coast, sea otters may be approaching a food- and habitat-limited distribution and carrying capacity, suggesting that the current status of sea otters in lower Cook Inlet is likely to remain similar in the absence of significant changes to prey, habitat, predation, or anthropogenic disturbance.

Alaska

Reproduction, growth, and tissue residues of deer fed dieldrin

Feeding tests were conducted from January, 1966, to January, 1969, to ascertain the effects of daily ingestions of sublethal amounts of dieldrin on white-tailed deer ( Odocoileus virginianus ). Groups of deer on 0 ppm dieldrin (controls), 5 ppm, and 25 ppm dieldrin were maintained at these respective levels, as were their progeny. Treated food was readily accepted. Dieldrin intoxication was not observed, and 9 of 10 animals of each group survived 3 years of treatment. No differences in conception or in utero mortality were found between groups. Fawns from dieldrin-fed does were smaller at birth and greater post-partum mortality occurred. Fertility of male progeny was not affected. Growth was slower and remained reduced in dieldrin-treated females which were immature when the study began. Hematologic values and serum protein concentrations were not significantly ( P > 0.05) related to treatment. Liver/body weight ratios were significantly ( P < 0.05) larger for the 25-ppm-dieldrin group. Pituitary glands were smaller and thyroids were larger in dieldrin-fed deer. Weight gains of fawns were significantly ( P < 0.05) reduced 2 of 3 years in dieldrin-treated groups. Placental transfer of dieldrin occurred. Whole milk from does fed 25 ppm dieldrin contained residues of 17 ppm. Residue levels in brain, liver, and thigh muscle tissues showed no evidence of increasing with length of treatment, but showed definite relationships to levels of dieldrin in daily diets. Nursing fawns had higher residues in brain tissues than did older deer on 5 ppm a d 25 ppm dieldrin. Highest brain residues (12.60 and 12.10 ppm, wet weight) occurred in fawns only a few days of age at death. Equilibrium between ingestion and storage or excretion of dieldrin occurred prior to 200 days and continued until nearly 1,100 days. There was no evidence of a sharp decline in residues after a long period of continued dosage. Daily ingestion of 100 and 200 ppm of dieldrin proved fatal to yearling male deer at 27 and 22 days, respectively.

Journal of Wildlife Management

Occurrence of anthropogenic organic compounds and nutrients in source and finished water in the Sioux Falls area, South Dakota, 2009-10

Anthropogenic organic compounds (AOCs) in drinking-water sources commonly are derived from municipal, agricultural, and industrial wastewater sources, and are a concern for water-supply managers. A cooperative study between the city of Sioux Falls, S. Dak., and the U.S. Geological Survey was initiated in 2009 to (1) characterize the occurrence of anthropogenic organic compounds in the source waters (groundwater and surface water) to water supplies in the Sioux Falls area, (2) determine if the compounds detected in the source waters also are present in the finished water, and (3) identify probable sources of nitrate in the Big Sioux River Basin and determine if sources change seasonally or under different hydrologic conditions. This report presents analytical results of water-quality samples collected from source waters and finished waters in the Sioux Falls area. The study approach included the collection of water samples from source and finished waters in the Sioux Falls area for the analyses of AOCs, nutrients, and nitrogen and oxygen isotopes in nitrate. Water-quality constituents monitored in this study were chosen to represent a variety of the contaminants known or suspected to occur within the Big Sioux River Basin, including pesticides, pharmaceuticals, sterols, household and industrial products, polycyclic aromatic hydrocarbons, antibiotics, and hormones. A total of 184 AOCs were monitored, of which 40 AOCs had relevant human-health benchmarks. During 11 sampling visits, 45 AOCs (24 percent) were detected in at least one sample of source or finished water, and 13 AOCs were detected in at least 20 percent of all samples. Concentrations of detected AOCs were all less than 1 microgram per liter, except for two AOCs in multiple samples from the Big Sioux River, and one AOC in finished-water samples. Concentrations of AOCs were less than 0.1 microgram per liter in more than 75 percent of the detections. Nutrient concentrations varied seasonally in source-water samples from surface water and groundwater. In the Big Sioux River, nitrite plus nitrate concentrations were typically less than 1 milligram per liter as nitrogen, and reached a maximum of 4.06 milligrams per liter as nitrogen following a June 2010 storm. Nitrite plus nitrate concentrations in groundwater ranged from less than 0.1 to 0.701 milligram per liter as nitrogen. Eight of the AOCs detected have a human-health benchmark that could be used to evaluate the concentrations in a human-health context. Four AOCs had maximum concentrations within an order of magnitude of the benchmark, indicating that additional monitoring of the compound may be warranted. Three herbicides (atrazine, metolachlor, and prometon) and one degradate (deethylatrazine) were detected in finished-water samples as frequently as in source-water samples. The concentrations of herbicides in source water varied by an order of magnitude from the period of peak use (early summer) to the winter months. Groundwater and finished-water concentrations of atrazine were similar for the six sampling dates when groundwater was the only source water used. Upstream wastewater discharges contributed a fairly small percentage of the flow to the Big Sioux River near Sioux Falls, but several AOCs associated with wastewater were frequently detected. The interpretation of all potential sources of nitrogen cannot be accomplished by use of nitrogen and oxygen isotopes in nitrate alone, but provides a qualitative indication that very little nitrate originates from excess fertilizer runoff, and most nitrate originates from municipal wastewater effluent, manure runoff (either from field application or feeding operations), or fertilizers mineralized by processes in the soil.

South Dakota

Estimates of soil ingestion by wildlife

Many wildlife species ingest soil while feeding, but ingestion rates are known for only a few species. Knowing ingestion rates may be important for studies of environmental contaminants. Wildlife may ingest soil deliberately, or incidentally, when they ingest soil-laden forage or animals that contain soil. We fed white-footed mice ( Peromyscus leucopus ) diets containing 0-15% soil to relate the dietary soil content to the acid-insoluble ash content of scat collected from the mice. The relation was described by an equation that required estimates of the percent acid-insoluble ash content of the diet, digestibility of the diet, and mineral content of soil. We collected scat from 28 wildlife species by capturing animals, searching appropriate habitats for scat, or removing material from the intestines of animals collected for other purposes. We measured the acid-insoluble ash content of the scat and estimated the soil content of the diets by using the soil-ingestion equation. Soil ingestion estimates should be considered only approximate because they depend on estimated rather than measured digestibility values and because animals collected from local populations at one time of the year may not represent the species as a whole. Sandpipers ( Calidris spp.), which probe or peck for invertebrates in mud or shallow water, consumed sediments at a rate of 7-30% of their diets. Nine-banded armadillo ( Dasypus novemcinctus , soil = 17% of diet), American woodcock ( Scolopax minor , 10%), and raccoon ( Procyon lotor , 9%) had high rates of soil ingestion, presumably because they ate soil organisms. Bison ( Bison bison , 7%), black-tailed prairie dog ( Cynomys ludovicianus , 8%), and Canada geese ( Branta canadensis , 8%) consumed soil at the highest rates among the herbivores studied, and various browsers studied consumed little soil. Box turtle ( Terrapene carolina , 4%), opossum ( Didelphis virginiana , 5%), red fox ( Vulpes vulpes , 3%), and wild turkey ( Meleagris gallopavo , 9%) consumed soil at intermediate rates. Ingested soil may be the principal means of exposure to some environmental contaminants or the principal source of certain minerals. Soil-ingestion estimates may be required for risk assessments of wildlife inhabiting contaminated sites and for computing budgets of those nutrients associated mainly with soil.

Colorado, Kansas, Louisiana, Maine, Maryland, Minn

Mountain Lions of the Flagstaff Uplands: 2003-2006 Progress Report

Executive Summary Stakeholders in management of mountain lions in the Flagstaff Uplands of northern Arizona have expressed increasing concern about both potential impacts of humans on lions and potential risks posed by lions to humans. A series of human-mountain lion encounters during 2000-2001 on Mt. Elden, immediately adjacent to Flagstaff, and similar incidents during 2004 near Tucson brought increased attention to management of human safety in mountain lion range. These human-centered concerns, together with long-standing questions about how the human infrastructure centered on Flagstaff might be affecting lion movements led us to initiate a mountain lion study in 2003 which we plan to continue through 2009. Our study focuses on movements and other behaviors of mountain lions, with the goal of providing information that can be used to increase human safety, decrease human impacts, and, overall, provide insight into the ecology of lions in this region. To serve this goal, we have focused on collecting data that will be the basis of explanatory models that can provide spatially-explicit predictions of mountain lion activity, specify the effects of human facilities, such as highways and urban areas, and provide insight into when, where, and how often different kinds of lions kill different kinds of prey. During 2003-2006, we captured six female and five male mountain lions in the Flagstaff Uplands, 10 of which we fitted with collars that collected up to six high-precision GPS fixes per day, transmitted daily to our offices via Argos satellites. This timely delivery of data allowed us to visit kill sites and other foci of localized activity to collect detailed information on lion behavior. By June 2006 we had obtained 9357 GPS locations and visited 394 sites, at which we documented 218 kills, 165 of which were by five females and 53 by five males. These data were the basis for preliminary analyses presented in this report. All lions during all seasons exhibited a strong selection for rough terrain and forest or woodland cover. Females differed from males by selecting more strongly for intermediate, rather than extreme, levels of terrain roughness, by selecting more strongly for chaparral vegetation and related rocky areas during winter, and by not selecting as strongly for areas near water sources. Overall, lions collared during this study strongly avoided flat open areas in private ownership. Male but not female lions exhibited pronounced selection for National Park Service jurisdictions. Both males and females year-round avoided residential areas and a zone outward to about 1-3 km and, when within this zone, moved more slowly and with less change in direction compared to when farther away. Collared lions have so far rarely crossed paved highways of any description - orders of magnitude less often than expected by chance. We observed only 3 crossings of an interstate highway, all on I17 and none on I40. Elk comprised the majority (52%) of kills by lions in our study, followed by mule deer (46%), and small mammals (15%). Adults comprised most of the mule deer kills (68%) and mesocarnivores, primarily coyotes (n = 21), comprised 73% of smaller prey. Calf and short-yearling elk comprised the largest single category of kills (29%). In addition to kills, we documented seven instances of scavenging, involving four different lions. Females differed from males by killing more mule deer and virtually all of the mesocarnivores, and by killing fewer elk of all ages. Intervals between kills averaged between 144 hrs (young females) to 221 hours (adult females), whereas average time spent on a kill ranged from 19 hrs (adult males) to 40 hrs (young males). Carcass mass had a strong effect on likelihood that a lion would bury or relocate a kill, the percentage of edibles consumed, and overall time spent feeding. Time spent feeding and likelihoods of carcass burial and relocation all peaked at intermediate carcass masses, suggesting an optimal mass in the range of 50-150 kg, likely dictated as much by handling efficiencies and competition from other scavengers as by a lion's shear ability to kill prey. Adult male lions exhibited a life strategy distinctly different from all other sex-age classes that entailed moving more rapidly over larger areas, and spending less time on kills in which they invested less energy handling, but from which they consumed tissue at a higher sustained rate.

Open-File Report

Effects of heptachlor-contaminated earthworms on woodcocks

The effects on woodcocks ( Philohela minor ) of eating heptachlor-contaminated earthworms were studied experimentally in a series of feeding trials in Louisiana in the winter of 1960-61. Six of 12 woodcocks fed worms which had been contaminated at an average of 2.86 ppm of heptachlor epoxide died within 35 days; 4 more had died by the fifty-third day, when the other 2 were killed for analysis. Worms from areas in Louisiana treated with 2 pounds of heptachlor per acre often contain more than 3 ppm of heptachlor epoxide. Eleven of 12 woodcocks fed worms contaminated at an average of 0.65 ppm survived the full 60 days of the experiment; one died on the forty-fifth day, apparently from other causes. All 11 untreated birds survived. Survivors were kept on one-quarter rations of untreated worms for 11 days. Two woodcocks, untreated previously, died during this starvation period. Five previously treated died; two were observed in spasms at death, and these contained 5.9 and 7.2 ppm heptachlor epoxide in their tissues, suggesting that the previous contaminated diet may have influenced mortality, even though the difference between two of nine dying and five of nine dying is not statistically significant. Surviving starved birds given an unrestricted supply of treated or untreated worms for 5 days survived and gained weight. Residues accumulated in their tissues in this time approached levels in birds that died of heptachlor poisoning. Residues in tissues of birds with different histories suggested residue loss at a rate of approximately 2.8 percent per day. Toxicant absorption was estimated to be in the approximate range of 16-20 percent. Residues in birds fed worms containing 0.65 ppm heptachlor epoxide were in the same general magnitude as those in field-caught birds, suggesting a similar average contamination of food supply. Weights and weight changes did not differ significantly between untreated birds and those receiving the lower level of toxicant. Among birds on one-quarter rations, the percentage of weight that could be lost without danger seemed to be near 20 percent. Woodcocks ate 18-208 grams of worms per day (average, 121 grams), representing 11-143 percent (average, 77 percent) of their body weights; birds ate contaminated and uncontaminated food in essentially equivalent amounts. Symptoms of heptachlor poisoning differed considerably between birds.

Journal of Wildlife Management

Application of the Stream Salmonid Simulator (S3) model to assess fall Chinook salmon (Oncorhynchus tshawytscha) production in the American River, California

Executive Summary Anadromous fish returning to the lower American River are restricted to 36 kilometers of free-flowing river between Nimbus Dam and American River’s confluence with the Sacramento River, California. Salmon in the American River provide an important freshwater recreational fishery. However, annual salmon production in the American River in recent years has been low relative to the mid-1990s (Surface Water Resources, Inc., 2001). To investigate the low production of fall-run Chinook salmon ( Oncorhynchus tshawytscha ), the Bureau of Reclamation requested that the U.S. Geological Survey apply the Stream Salmonid Simulator (S3) model to the population of fall-run Chinook salmon on the American River. The American River was chosen among seven candidate Sacramento Basin rivers for S3 application. The American River was selected because of its management and public interest, recently low anadromous fish production, and rich time series of key demographic data needed for S3 application. Data that were not available, however, were empirical estimates on juvenile salmon habitat suitability in the American River. Therefore, a large component of applying S3 to the American River was devoted to the estimation of juvenile salmon habitat suitability and capacity. This entailed snorkeling the lower American River for 3 weeks in March 2021 during the early out-migration period for juvenile Chinook salmon. These efforts were fruitful and showed that the typically small fish (<55 millimeters) in the American River preferred much shallower depths than predicted by habitat suitability criteria derived from the literature for this population. Having empirical estimates on juvenile salmon in the American River provided a solid foundation from which to simulate the population using the S3 model. The S3 model is a spatially explicit population model that runs on a daily time step to simulate redd superimposition, egg maturation, fry emergence and the subsequent growth, survival, and emigration of juvenile Chinook salmon from the river. The key features of this model relevant to this report include (1) a temperature-dependent bioenergetics model driving daily growth rates; (2) density-dependent dynamics that are influenced by the effect of flow on suitable habitat area; and (3) within-year habitat, river flow, and water temperature effects specific to spawning, egg incubation, and fry, parr, and smolt life stages. We used estimates of spawning escapement and geo-referenced redd locations to quantify the spatial and temporal distribution of female spawners for brood years 2014–19. These estimates of female spawners initiate the simulation of each year’s juvenile salmon emergence and emigration over a spatial domain extending from Nimbus Dam to the river’s confluence with the Sacramento River. Using weekly estimates of juvenile salmon abundance and size (fork length) that passed the Watt Avenue fish trap (river kilometer 14.7), we calibrated the S3 model by estimating three key demographic parameters for each year, y : (1) S y , the average daily survival probability, (2) M 0y , the intercept for density-dependence in movement, representing the average daily probability of remaining in a habitat at zero abundance, and (3) C y , the average daily proportion of maximum consumption. These parameters were obtained by minimizing the Mallow’s distance (Lupu and others, 2017) between distributions of weekly abundances and sizes of fish at the traps and weekly simulated abundances and sizes (by S3). Investigation of model fit showed excellent agreement between simulated annual abundances and the abundance of fish passing the fish trap. However, when we compared weekly abundances at the fish trap, S3 under-predicted peaks and over-predicted troughs in the time series of weekly abundances at the fish trap. Thus, some unknown within-year effects have yet to be identified and incorporated in the S3 model. Identifying these important effects and incorporating them in the S3 model would help explain the lack of fit between estimated and simulated weekly abundances. We estimated parameters for 6 years that included a wide range of female spawner abundances (3,057–10,753) and water year types (Critical–Wet). We contrast our estimated parameters to the corresponding number of female spawners and the water year type for the Sacramento Valley. By happenstance, years having higher annual spawner abundances concurred with Critical to Dry water year types. Estimates of survival trended lower with higher spawner abundances and Critical to Dry conditions. In contrast, the extremely wet water year of 2017 had the lowest M 0y , suggesting less density-dependence in fish movement, and the lowest C y , suggesting lower average consumption in this year. When this high-flow year was excluded, a trend towards higher probabilities of fish remaining in a habitat at low abundance and lower proportions of maximum consumption was apparent from Critical to Wet conditions, but only 5 years of data were included. Except for 2017, daily proportions of maximum consumption were relatively high ( C y > 0.83), suggesting that fish were feeding at reasonably high proportions relative to the expected maximum consumption as defined by the “Wisconsin” bioenergetics model (Stewart and Ibarra, 1991). Survival estimates from fry emergence to outmigration at the Sacramento River confluence were generally low when integrated over time. The highest daily survival probability was S y = 0.93 in 2019, or 50 percent total mortality after 10 days. In contrast, our lowest daily survival probability was S y = 0.74 in 2015, or 95 percent total mortality after 10 days. Consequently, even our highest estimated daily survival probability might be considered low. This is especially true given that S y was estimated over a relatively short distance (<14.7 kilometers) from emergence to the Watt Avenue fish trap. Several factors, including our assumed and relatively high daily egg survival rate of 0.9975, could influence juvenile survival estimates. For example, an egg survival rate of 0.9975 results in 3-percent total mortality after 10 days. Egg mortality estimates used in S3 calibration were approximated from egg survivorship studies in the Yakima River, Washington (Johnson and others, 2012), and remains one of the greater uncertainties in S3 when estimating survival across life stages. By including bona fide estimates of egg survival in S3 simulations, the validity of the S3’s current daily egg survival rate could be assessed specifically for the American River. Tagging studies also could provide S3 with direct estimates of juvenile survival and movement; survival during egg incubation then could be estimated indirectly via model fitting.

California

Fishes of Missouri River, chute, and flood plain habitats: Chapter 4 in Initial biotic survey of Lisbon Bottom, Big Muddy National Fish and Wildlife Refuge

The Lisbon Bottom Unit of the Big Muddy National Fish and Wildlife Refuge (Refuge) is approximately 2,200 acres and is the first complete unit of the Refuge. Primary objectives of the Refuge are to create and restore diverse riverine aquatic habitats and reconnect the Missouri River to its flood plain where feasible. Management seeks to accomplish these objectives by encouraging natural processes of erosion, deposition, and succession to the greatest extent possible. One of the most salient aquatic features of the Lisbon Bottom Unit is a newly created 2-mile-long free-flowing chute, or side channel (Fig. 1). This chute began forming as a levee breech scour hole during the Great Flood of 1993. The chute continued to develop during the 1995 flood and finally cut through to a flowing side channel during the 1996 flood. Extensive erosion and bank sluffing continued during 1997 due to sustained high flows that occurred throughout most of the year. The chute has progressively become wider and deeper with a developing meander pattern and channel bars have begun to form. Lisbon Bottom also contains several seasonal and permanent wetlands and is subject to periodic flooding at high Missouri River stages. Eight studies have been completed or are ongoing to evaluate Missouri River fishes associated with various habitat components of Lisbon Bottom and adjacent Missouri River reaches (Table 1). Several are part of much larger investigations to evaluate fish use of flood-created habitat features, basinwide fish assessment, and endangered or candidate species concerns. At the Lisbon Bottom Unit or in the Missouri River adjacent to the unit 54 fish species were collected (Table 2). Eight of these species have either a protected status under State or Federal laws or biologists consider them to potentially qualify for protected status. The status of the following fish is listed in Table 2: pallid sturgeon x shovelnose sturgeon hybrid, paddlefish, northern pike, sturgeon chub, sicklefin chub, ghost shiner, western silvery minnow, plains minnow, and blue sucker. Equally important to note are fish species that were not collected. The flathead chub, currently listed as State endangered and proposed for Federal listing, were not collected in any of the studies. Common fish species, collected in all seven sampling studies, included gizzard shad, common carp, river carpsucker, and freshwater drum. Paddlefish, skipjack herring, silver carp, white sucker, redfin shiner, western silvery minnow, bullhead minnow, and black bullhead were each collected in only one of the seven studies. Pflieger (1971) developed a guild system for the fishes of Missouri based on distribution patterns and centers of abundance. Fish were assigned to one of four primary faunal groups: Ozark, Big River, Lowland, and Prairie. Two secondary groups (Ozark-prairie and Ozark-lowland) were defined for species equally abundant and distributed in two of the primary areas. Two Ozark-prairie species, shorthead redhorse and white sucker, were collected on or near the Lisbon Bottom Unit. Big River species are found primarily in the Missouri and Mississippi Rivers. The environmental factors controlling fish distribution in these rivers appear to be substrate, current velocity, and turbidity. On or near the Lisbon Bottom Unit 18 Big River species were collected (Table 2). Lowland species are intolerant of siltation and high turbidity. They inhabit standing or slow-moving water with sand, fine gravel, and organic debris substrates. Two Lowland species, bullhead minnow and mosquitofish, were collected in connected scours on the Lisbon Bottom Unit. Prairie species have broader ecological tolerances than the Ozark and Lowland species. They are largely absent in high gradient streams and cool, clear waters. Prairie species make up a significant proportion of Missouri and Mississippi River fishes. Seven Prairie species were collected on or near the Lisbon Bottom Unit (Table 2). The last group, Wide-ranging, was defined as the species more widespread than the other faunal groups with broader environmental tolerances. Most Wide-ranging species occur at least occasionally in all sections of the state. Nineteen Wide-ranging species were collected on or near the Lisbon Bottom Unit (Table 2). Kubisiak (1997) found that isolated scours in the Lower Missouri River were dominated by Wide-ranging species. Connected scours also held Wideranging species but they contained Prairie species and were dominated by Big River species. Faunal group diversity and species richness were compared between isolated (Study l) and connected (Studies 4, 5, 7, and 8) scours of the Lisbon Bottom Unit. Species richness was similar with 22 species collected in isolated scours and an average of 24.8 species collected in connected scours. Isolated scour fish catches were dominated by Wide-ranging fish (59%) with 14% Big River fishes and 14% Prairie fishes. Connected scours had a larger percentage of Big River fish (32%) and fewer Wide-ranging (36%) and Prairie (13%) species than the isolated scours. In the combined studies, Wide-ranging fish dominated the connected scours. A similar comparison was made of fish from chute habitats and the Missouri River. Chute habitats include the Lisbon Bottom Chute, the chute between the right bank and island located at river mile 219, and a shallow channel running between a large sandbar complex and the channel border adjacent to Lisbon Bottom. Species richness in the chutes (35 species) was greater than that of the adjacent river (23 species). Big River species (40%) dominated the chute samples, followed by Wide-ranging at 31% Cand Prairie Cut 14%. The river samples contained equal numbers of Wide-ranging species (35%) and Big River species (35%) with 17% Prairie species. Differences in fish guild diversity and species richness may be due to numerous factors including sampling effort and sampling season. River samples were collected only in Study 3 in October; chute samples were collected in July, August, and October. Fish may have been using the chute in July and August to spawn, feed, or escape high river levels. The Lisbon Bottom Chute is a truly unique feature of the Lower Missouri River. It is the first naturally created side channel to develop since the U.S. Army Corps of Engineers tamed the river. The Corps eliminated 89% of the islands in the Missouri River between 1879 and 1954 (Funk and Robinson 1974). The chutes between the islands and the shore were shallower with less current than the main channel. These areas provided diversity to the Missouri River fish habitat, serving as nursery and feeding areas. The Lisbon Bottom Unit of the Big Muddy National Fish and Wildlife Refuge will continue to be studied in an effort to understand the complex role these areas play in river systems.

Missouri