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Mortality was high in an experimental group of rainbow trout ( Salmo gairdneri ) fed estradiol as part of a sex reversal study. Pathology included hypertrophy of the kidneys and liver and heavy accumulations of presumptive vitellogenin.
Experiments were conducted to determine the effect of level of feeding (restricted feeding and feeding to satiation) and dietary variations in the balance of monovalent minerals on the arginine requirement of rainbow trout fingerlings. Based on growth and efficiency of feed utilization for growth, the arginine requirement was lower (3.5% of protein) when fish were fed to satiation than when they were fed at a restricted level (4.2% of protein). When calculated on the basis of the amount of arginine consumed per day, however, the requirements were similar for fish subjected to the two methods of feeding. Three balances (acidic, neutral, and alkaline) of sodium, potassium and chloride were used in several experiments. The arginine requirement tended to be higher when fish were fed diets containing the alkaline as compared to the acidic balance of minerals. These studies indicate that the method of feeding has a significant effect on the dietary concentration of arginine needed to maximize growth rate and feed utilization in rainbow trout.
Studies were conducted to determine the value of lupin flour as a replacement for full-fat soybean meal (FFSM) in the diet of rainbow trout ( Salmo gairdneri ). Energy and digestibility values for lupin were determined using a metabolism chamber and it was found that trout utilize lupin well. The gross energy, metabolizable energy, and the percent digestibilities of protein and energy for lupin were found to be 4757 kcal/kg, 2797 kcal/kg, 85.2%, and 66.1%, respectively. When plant protein (either FFSM or lupin flour) was used as a supplemental dietary protein source to fish meal, or when the plant protein was the primary dietary protein source, fish fed diets where lupin completely replaced FFSM gained 99–106% of the weight of those fed the FFSM and had statistically equal feed efficiency ratios ranging from 80 to 83%. Although some minor dietary alterations may be needed (e.g., lowering the fat content of the diet) to maintain the economic advantage of lupin over soybean meal, lupin appears to be a useful source of protein for trout and may represent another economical plant protein alternative to fish meal.
Loma salmonae (Putz et al., 1965) infections were observed in five groups of coho salmon, Oncorhynchus kisutch , reared in seawater net-pens in Washington State, U.S.A. in 1984–1986. Ultrastructural characteristics, size of spores, tissues and host infected, and geographical location identified the microsporidium as Loma salmonae . Preserved spores measured 4.4×2.3 (4–5.6×2–2.4) μm and exhibited 14–17 turns of the polar filament. Infections were evident in the gills of some fish before seawater entry, but few parasites were observed and they caused little tissue damage. Infections observed in fish after transfer to seawater were associated with significant pathological changes in the gills. A mixed inflammatory infiltrate was associated with ruptured microsporidian xenomas within the vessels and interstitium of the primary lamellae. Microsporidian spores were dispersed throughout the lesions and were often seen inside phagocytes. The parasite was also observed in the heart, spleen, kidney and pseudobranchs; however, the inflammatory lesions were common only in the heart. Monthly examination of fish after transfer to seawater showed peak prevalences (33–65%) of gill infections during the summer. Although moribund fish were often infected with other pathogens, the high prevalence of L. salmonae infections and the severity of the lesions it caused, suggested that this parasite significantly contributed to the recurrent summer mortalities observed at this net-pen site.
The nutritional value of Artemia nauplii from the Great Salt Lake was effectively improved for larval striped bass ( Morone saxatilis ) by incorporation of unsaturated fatty acids (20: 5 n -3 and 22: 6 n -3) into the nauplii by the direct method of enrichment. Survival at 24 days post-hatch increased from 23% to 64% when fish were fed nauplii containing 8.24% lipid as 20: 5 n -3 and 3.10% as 22: 6 n -3 fatty acids. Growth of the larval fish was significantly improved by a diet of fatty acid enriched nauplii. Fatty acid composition of the larvae reflected the composition of the fed nauplii. Results suggested a requirement by larval striped bass for long-chain highly unsaturated fatty acids and an inability, at this stage of development, to elongate and desaturate the shorter chain fatty acids in sufficient amounts to meet this requirement.
Rainbow trout were fed diets with graded levels (0.6%, 1.6%, 2.5%, and 4.1%) of a yeast nucleic acid extract corresponding to dietary Saccharomyces cerevisiae levels of 7.5%, 20%, 30% and 50% or diets supplemented isonitrogenously (0.8% N) with free purines (adenine, guanine, xanthine, and hypoxanthine) in two 12-week studies. Fish fed increasing levels of yeast extract manifested significant ( P < 0.05) corresponding incremental increases in growth and nitrogen retention. No negative effects on feed intake were observed. When fish were fed supplemental free purines, adenine was shown to be a potent inhibitor of feed intake and growth. While supplementation with the remaining purines did not negatively affect feed intake or growth, carcass nitrogen retention was significantly depressed, indicating the lack of a nitrogen-sparing effect. Our results indicate that the nutritional significance of free dietary adenine versus nucleic acid-bound adenine in yeast or yeast nucleic acid is an important consideration in evaluating the suitability of single cell proteins in fish feed formulations.
The distribution and loss of radioactivity from tissues were determined in 60 juvenile channel catfish (Ictalurus punctatus) following oral dosing with the candidate fish therapeutant Sarafin® ([ 14 C] sarafloxacin hydrochloride) at 10 mg/kg for 5 consecutive days. Twelve groups of 5 fish each were sampled at selected times ranging from 3 to 240 h after the last dose was administered, The concentration and content of sarafloxacin-equivalent activity was determined in liver, gallbladder, kidney, skin, and skinless fillet by sample oxidation and liquid scintillation counting; content of sarafloxacin-equivalent activity was determined in stomach and anterior and posterior intestines, Skinless fillet tissues were also analyzed for sarafloxacin and for potential metabolites by gradient-elution high-performance liquid chromatography (HPLC) with in-line radiometric and fluorescence detection, Loss of radioactivity from the whole body conformed to a bimodal elimination pattern with a rapid initial phase ( t 1/2 =11 h) and a slower secondary phase ( t 1/2 =222 h). Tissue and contents of the gastrointestinal tract (i.e. stomach and anterior and posterior intestines) were a principal depot of activity during the first four sample times (3, 6, 12, and 24 h); the combined head, skeleton, and fins (i.e. residual carcass) were the principal depot of activity in samples taken after 24 h. Of those tissues sampled 3 h after the last dose, relative sarafloxacin concentration was greatest in the liver (4.06 μg equivalents/g) and least in the residual carcass (1.13 μg equivalents/g), Intermediate concentrations were found in the kidney (2.04 μg equivalents/g), skinless fillet (1.71 μg equivalents/ g), and the skin (1.51 μg equivalents/g). Concentrations of sarafloxacin-equivalent residues in edible skinless fillet were consistently among the lowest of all tissues examined. The highest mean concentration of parent-equivalent material in the fillet tissue was found 12 h after administration of the last dose (2.27 μg equivalents/g) and declined thereafter, Sarafloxacin constituted between 80 and 90% of the extractable radioactive residues from the fillet homogenates. No other peaks were resolved in any of the fillet tissue samples analyzed by HPLC with in-line radiometric detection.
Segregation of spring chinook salmon ( Oncorhynchus tshawytscha ) brood stock based on the measurement of maternal Renibacterium salmoninarum infection levels by the enzyme-linked immunosorbent assay (ELISA) and the fluorescent antibody technique (FAT) was previously shown to affect the prevalence and levels of bacterial kidney disease (BKD) in progeny fish during hatchery rearing. Smolts from that study were subjected to standardized fish health and condition evaluation procedures 2 weeks before the conclusion of hatchery rearing and release of the fish for migration to the Pacific Ocean. The results suggested that the general health of the smolts in the progeny group from parents that had low R. salmoninarum infection levels or tested negative for R. salmoninarum (low-BKD group) was better than that of the smolts in the progeny group from female parents with high R. salmoninarum infection levels (high-BKD group). Testing by the ELISA showed that the overall severity of R. salmoninarum infection also was lower in the smolts from the low-BKD group. Subgroups of smolts from the study were acclimated to tanks of seawater for extended holding. After a 22-day acclimation period and 98 days in full-strength (29 ppt salinity) seawater, total mortality was 12% in the low-BKD group and 44% in the high-BKD group. All of the mortality in the low-BKD group and 85% of the mortality in the high-BKD group occurred after the fish were transferred to full-strength seawater. Testing of kidney tissues from all dead fish by the FAT revealed that 85% of the fish that died in the high-BKD group had high R. salmoninarum numbers, indicating that BKD was the cause of death. In contrast, none of the fish that died in the low-BKD group had detectable numbers of R. salmoninarum . We concluded that brood stock segregation by use of the ELISA and the FAT can affect mortality and the R. salmoninarum status of progeny chinook salmon for as long as 21 months after hatching, even after the fish have been transferred to seawater.
Groups of juvenile spring chinook salmon naturally infected with Renibacterium salmoninarum , the causative agent of bacterial kidney disease, were fed diets containing different levels of vitamin E and selenium for 214 days in fresh water and 110 days in seawater. The fish were fed vitamin E at concentrations of either 53±3 mg (designated e ) or 299±9 mg (designated E ) α -tocopheryl acetate equivalence/kg dry diet in combination with sodium selenite to give selenium concentrations of either 0.038±0.008 mg (designated s ) or 2.49±0.15 mg (designated S )/kg dry diet. No mortality occurred in the group fed the diet, whereas mortality was 3% in the groups fed the and diets, and 31% in the group fed the diet. At the end of the experiment, weight gain and hematocrit values were significantly greater in those fish fed the E diets compared with those fed the e diets, whereas the hepato-somatic index was significantly higher in fish fed the e diets. Glutathione peroxidase activity in blood plasma was significantly higher in fish fed the S diets compared with those fed the s diets. No definite effect of dietary vitamin E and selenium on the prevalence and severity of natural R. salmoninarum infections was demonstrated.
Aeromonas salmonicida is a principal pathogen that contributes to mortality in hatchery-maintained Atlantic salmon that originated from the Connecticut and Merrimack rivers in the northeastern United States. Field tests were conducted to assess a filtration method for detection of A. salmonicida in water used to rear fishes. A presumptive identification of typical A. salmonicida was available within 72 h and the pathogen was confirmed within 96 h. This filtration method has been incorporated into daily management practices and provides results that allowed managers to make more informed decisions for fishery resources.
Since the fry of certain species of fish cannot tolerate even slight amounts of supersaturation, their water supplies must be degassed. Gas content can be reduced to subsaturated, sublethal levels by passing it through the vacuum degasser described here. The system includes a low-cost, flow-through unit easily capable of degassing (to less than saturated conditions) water flowing at 190 liters min −1 . In tests completed to date, dissolved oxygen content was never reduced below 7 ppm.
A packed column oxygen obsorber was developed in which oxygen flow is directed, in serial reuse, through parallel packed column stages receiving equal portions of the liquid being treated. The relative performance of the absorber was established using a computer simulation program employing finite difference-mass transfer calculations. The program was calibrated using packing specific mass transfer coefficients derived from pilot scale test data. A separate series of tests served to verify model assumptions and performance predictions. Simulation data indicated multi-stage operation can substantially reduce the column height required to achieve a selected oxygen absorption efficiency (AE); for example, the column height required to achieve an AE of 76·5% with an inlet volumetric oxygenwater ratio of 0·008 (column packing, 3·81 cm plastic ACTIFIL ® ; water temperature, 20°C; influent dissolved oxygen, 9·08 mg/litre; operating pressure (absolute), 760 mm Hg) was 0·27 m using a 10-stage system versus 1·39 m using a single-stage absorber. Reductions in column height achieved were related to oxygen and water feed rates, number of stages employed, mass transfer characteristics of the column packing used, and concentrations of dissolved gases in the liquid being treated.
A numerical model was used to evaluate the performance of a surface agitation system designed to contact commercial oxygen with water. The modeled system was unique in that oxygen-rich off-gas, normally discharged to the atmosphere, was directed in serial reuse through additional contact stages receiving untreated water. A correlation between the agitator mass-transfer coefficient and power demand, needed to calibrate the model, was established using a single-stage (37 W) contactor of 1·18 m 3 capacity. Additional tests, conducted with both single and three-stage equipment, verified model assumptions and performance predictions. Simulation runs indicated oxygen flow or power input required to meet a given effluent dissolved gas criterion can be substantially reduced by the off-gas reuse step; for example, to achieve an effluent dissolved oxygen of 24·1 mg/litre with a single stage agitator the oxygen feed rate needed was 61·5% greater than that required by a six-stage system receiving the same total power input (standard aeration efficiency, 0·5 kg/kW h; water flow rate, 100 litre/min; influent dissolved oxygen, 9·08 mg/litre at 15°C). The savings achieved increased with (1) greater target effluent dissolved oxygen concentrations, (2) lower oxygen feed rates, (3) higher input power levels, and (4) number of contact stages.
Successful application of the packed column in fish culture requires a design based not only on oxygen supplementation rates (kg/day), or other standard performance indicators such as oxygen absorption efficiency (kg absorbed/kg applied) or transfer efficiency (kg/kWh), bvt also on dissolved nitrogen and total dissolved gas pressure limits and predicted changes across the system. Such a design procedure was developed by applying a mass balance on the gas and liquid phases of the system along with Henry's law and previously published packing-specific mass-transfer correlations. The design approach is unique in that column pressure and oxygen feed rates are calculated for target changes in dissolved oxygen and nitrogen without the use of iterative numerical procedures. An alternative calculation sequence was developed to establish the sensitivity of column performance to changes in oxygen feed rates at a selected column pressure or packed bed depth.
A portable vacuum degasser was developed to satisfy seasonal hatchery pretreatment needs. Dissolved-gas pressures in water exiting a packed column were regulated automatically with a unique feedback control loop incorporating a gasometer, pressure transducer, electronic (PID) controller and an electrically actuated pump discharge valve. The flow capacity of the system was 160 l/min with a total power requirement of 0·81 kW. Following controller tuning, field tests demonstrated the ability of the feedback loop to set appropriate column vacuum levels quickly in response to varying inlet dissolved-gas pressures or changes in selected controller set points. The degasser's ability to reduce dissolved nitrogen (DN) and increase dissolved oxygen (DO) concentrations was also assessed at four or five pure-oxygen feed rates under each of four column vacuum levels (−4·5, −9·0, −14·6 and −20·0 cm Hg). Performance was then compared with that predicted with a multicomponent gas transfer model. Relative error of model projections averaged just 7·7% for DO and 2·3% for DN (n = 19). Effluent DN ranged between 60·6 and 96·4% of saturation concentrations at 8·8°C with the influent DN at 135% of saturation. The high DN desorption rates achieved allow side-stream pretreatment with blending.
Subadult rainbow trout ( Oncorhynchus mykiss ) stocked at 48 kg/m 3 (3 lb/ft 3 ) were subjected to treatments of tank design (rectangular plug flow, circular, and cylindrical cross flow) and water exchange rate (1·5 and 2·5 exchanges/h) to determine their effects on fish behavior, growth, and metabolism. Ambient light levels and current velocities were also measured in each of three tank sectors (upstream, middle, and downstream) to determine their relative contributions to behavioral effects. Tank design significantly affected fish orientation to current, contact time with tank surfaces, and frequency of agonistic encounters, though aggression levels were relatively low overall. Gradients in fish distribution by sector were greatest in plug-flow tanks. Effects were either modified or eliminated by increasing the water exchange rate from 1·5 to 2·5/h. Multiple-regression analysis showed the following hieararchy of independent-variable effects on fish distribution: tank type > exchange rate > aggression level > current velocity > light level. Significant effects of tank design were also observed on fish growth in terms of biomass gain (cross flow > plug flow > circular). These results were matched in metabolic studies, where both oxygen consumption and ammonia excretion were highest in circular and lowest in cross-flow tanks. Reduction (cross-flow compared with circular tanks) in oxygen consumption averaged 13·6%, ammonia excretion 17·5%. These results were also modified by an increase in water exchange rate. Tank-design effects on fish metabolism and growth may be mediated, at least partly, through changes in fish behavior.
Gas-phase axial dispersion (mixing of the composition of the gas phase along the longitudinal axis) was characterized in an enclosed spray tower for purposes of establishing reactor type for the solute-solvent pair oxygen and water. Test condition variables were spray tower height (TH), 1·52, 2·03 and 2·54 m; hydraulic loading (HL), 44·2, 66·3 and 88·4 kg/m 2 s; the ratio of volumetric oxygen injection to water flow rate (G/L), 1·0, 2·5 and 5·0%; the ratio of volumetric bulk tower gas recirculation flow rate to water flow rate (BG/L), 0, 500 and 700%; and bulk tower gas recirculation direction, counter-current to and co-current to the water flow. Gas composition measurements (% O 2 ) made across the long axis of the tower under steady-state conditions provided 1020 independent observations and 240 gas composition profiles. Factors showing a significant effect ( P < 0·05) on gas composition were TH, HL, G/L and BG/L. Sample location as a percentage of TH did not have a significant effect on gas composition and accordingly profile slopes were not different from zero ( P > 0·05). Profile data indicate a completely mixed gas phase within the tower. The dispersion observed was attributed to the lack of a significant pressure drop along the axis of the reaction vessel, forces due to nozzle operation, and to bulk tower gas recirculation.
Anadromous alosines are widespread throughout the Northern Hemisphere. Juveniles of this clade are notoriously fragile animals that are at high risk of injury and death associated with passage at hydroelectric facilities. Although turbine mortality is a common concern, conditions encountered when bypassed around these routes may also be hazardous. Downstream bypass structures typically discharge into plunge pools, which are highly turbulent and may cause mechanical injury. We subjected live, actively migrating juvenile blueback herring to a suite of realistic plunge pool conditions (3 m drop, pool depth of 60–180 cm, and discharge of 0.28–1.70 m 3 /s) and monitored them for ≥ 96 h. Survival was generally higher than expected (>80% in all cases). However, both plunge pool volume and total discharge affected survival with elevated discharge and shallow conditions associated with increased mortality. Mortality was often delayed: rates remained elevated throughout the monitoring period, indicating that survival studies based on shorter periods underestimate total mortality.