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Vascular Plant and Vertebrate Inventory of Saguaro National Park, Rincon Mountain District

Executive Summary This report summarizes the results of the first comprehensive inventory of plants and vertebrates at the Rincon Mountain District (RMD) of Saguaro National Park, Arizona. From 2001 to 2003 we surveyed for vascular plants and vertebrates (amphibians, reptiles, birds, and mammals) at the district to document the presence of species within its boundaries. Park staff also surveyed for medium and large mammals using infrared-triggered cameras from 1999 to 2005. This report summarizes the methods and results of these two efforts. Our spatial sampling design was ambitious and was one of the first of its kind in the region to colocate study sites for vegetation and vertebrates using a stratified random design. We also chose the location of some study sites non-randomly in areas that we thought would have the highest species richness. Because we used repeatable study designs and standardized field methods, these inventories can serve as the first step in a biological monitoring program for the district. We also provide an important overview of most previous survey efforts in the district. We use data from our inventory and other surveys to compile species lists and to assess inventory completeness. With the exception of plants, our survey effort was the most comprehensive ever undertaken in the district. We recorded a total of 801 plant and vertebrate species, including 50 species not previously found in the district (Table 1) of which five (all plants) are non-native species. Based on a review of our inventory and past research at the district, there have been a total of 1,479 species of plants and vertebrates found there. We believe inventories for all taxonomic groups are nearly complete. In particular, the plant, amphibian and reptile, and mammal species lists are the most complete of any comparably large natural area of the 'sky island' region of southern Arizona and adjacent Mexico. For each taxon-specific chapter we discuss patterns of species richness and environmental determinants of these patterns. For all groups except medium and large mammals, the low elevation stratum (<4,000 feet) contained the highest species richness, after accounting for differences in survey effort among strata. This is consistent with known patterns of species richness in the sky island mountain ranges. Using data on relative abundance for plants and birds, we were able to identify a number of distinct ecological communities, which were consistent with known patterns in the sky islands. Our review of species lists and park records reveals that the district has lost species, particularly plants and mammals, in the past few decades. Because of the district's close proximity to the rapidly growing city of Tucson, there are a number of development-related threats that could cause additional species loss or decline in abundance of some species. In particular, the increasing groundwater pumping near Rincon Creek, the most species-rich area in the park, is likely to impact the unique riparian vegetation and animals of that area. We discuss this and other demands on the ecological integrity of the district. We also recommend additional inventory, monitoring, and research studies.

Open-File Report↗

Divergent physiological responses of hydric and mesic riparian plant species to a Colorado River experimental flow

Riparian plant species can differ in their responses to streamflow variation in ways that strongly influence the composition and functioning of riparian plant communities. Quantifying these differences and the potential asymmetry of responses to low- versus high-flow phases of stream fluctuations is important for predicting and managing vegetation responses to variation in flow regimes. We measured the physiological response of two riparian plant species with different habitat preferences to an experimental flow that progressed from a low-flow to high-flow phase. Schedonorous arundinaceus , a hydric grass growing in near-channel habitat, exhibited significant and biologically substantial declines in stem water potential (SWP) during the low-flow phase of the experiment and a saturating increase in leaf relative water content (LRWC) during the high-flow phase. These patterns are consistent with cavitation risk in response to low-flow anomalies and saturating responses to high-flow anomalies in this hydric species. Pluchea sericea , a mesic shrub growing at intermediate elevations above the channel, exhibited a decrease in LRWC during the low-flow phase and an increase in SWP during the high-flow phase. These patterns are consistent with protection from stem cavitation risk during drought through leaf dehydration and opportunistic increases in water status in response to high-flow anomalies in this mesic species. The asymmetrical responses of both species to low- versus high-flow phases demonstrate unique physiological responses to flow anomalies of contrasting directions attributable to species habitat preferences and functional strategies that can be used by managers to predict non-linear vegetation responses to flow variation.

Plant Ecology↗

The role of hybridization in the distribution, conservation and management of aquatic species: Symposium review

This issue of Reviews in Fish Biology and Fisheries contains six papers addressing several critical aspects of hybridization in fishes and aquatic organisms. Hybridization is a phenomenon long recognized in fishes (Hubbs, 1920, 1955; Schwarz, 1981), as well as in other plant and vertebrate taxa, despite some rather dogmatic proclamations to the contrary, e.g., comments made by David Starr Jordan at the beginning of the 20th century that the species “line” is rarely crossed in fishes (Clark Hubbs, personal communication). Since that time, interspecific genetic introgression has been well documented in many fish genera and species: Barbus (Berrebi and CattaneoBerrebi, 1993); Cyprinodon (Echelle and Connor, 1989; Dowling and DeMarais, 1993); Gambusia (Hubbs, 1959; Scribner and Avise, 1994); Esox (Wahl and Stein, 1993); Lepomis (Avise et al., 1984); Luxilus (Duvernell and Aspinwall, 1995); Morone (Harrell et al., 1993); Notropis (Dowling et al., 1989; Dowling and Hoeh, 1991); Oncorhynchus (Busack and Gall, 1981; Campton and Utter, 1985; Loudenslager et al., 1986; Leary et al., 1987; Forbes and Allendorf, 1991; Dowling and Childs, 1992); Salmo (Nyman, 1970; Wilkins et al., 1993; Giuffra et al., 1996; Hartley, 1996; Perez et al., 1999); Salvalinus (Hammar et al., 1991; Bernatchez et al., 1995; Baxter et al., 1997; Glemet et al., 1998; Wilson and Bernatchez, 1998); Sebastes (Seeb, 1988); Stizostedion (Billington et al., 1988). See also reviews in Campton (1987), Verspoor and Hammar (1991), Smith (1992), and Scribner et al. (2000). More recently, a number of investigations have documented not only first generation hybrids, but also subsequent generation introgressant hybrids (Bartley et al., 1990; Verspoor and Hammar, 1991). As a result, our views about species typology and hybrids continue to change.

Reviews in Fish Biology and Fisheries↗

Measurement of soil carbon oxidation state and oxidative ratio by 13 C nuclear magnetic resonance

The oxidative ratio (OR) of the net ecosystem carbon balance is the ratio of net O 2 and CO 2 fluxes resulting from photosynthesis, respiration, decomposition, and other lateral and vertical carbon flows. The OR of the terrestrial biosphere must be well characterized to accurately estimate the terrestrial CO 2 sink using atmospheric measurements of changing O 2 and CO 2 levels. To estimate the OR of the terrestrial biosphere, measurements are needed of changes in the OR of aboveground and belowground carbon pools associated with decadal timescale disturbances (e.g., land use change and fire). The OR of aboveground pools can be measured using conventional approaches including elemental analysis. However, measuring the OR of soil carbon pools is technically challenging, and few soil OR data are available. In this paper we test three solid-state nuclear magnetic resonance (NMR) techniques for measuring soil OR, all based on measurements of the closely related parameter, organic carbon oxidation state (C ox ). Two of the three techniques make use of a molecular mixing model which converts NMR spectra into concentrations of a standard suite of biological molecules of known C ox . The third technique assigns C ox values to each peak in the NMR spectrum. We assess error associated with each technique using pure chemical compounds and plant biomass standards whose C ox and OR values can be directly measured by elemental analyses. The most accurate technique, direct polarization solid-state 13 C NMR with the molecular mixing model, agrees with elemental analyses to ??0.036 C ox units (??0.009 OR units). Using this technique, we show a large natural variability in soil C ox and OR values. Soil C ox values have a mean of -0.26 and a range from -0.45 to 0.30, corresponding to OR values of 1.08 ?? 0.06 and a range from 0.96 to 1.22. We also estimate the OR of the carbon flux from a boreal forest fire. Analysis of soils from nearby intact soil profiles imply that soil carbon losses associated with the fire had an OR of 1.09 1 (??0.00 3 ). Fire appears to be a major factor driving the soil C pool to higher oxidation states and lower OR values. Episodic fluxes caused by disturbances like fire may have substantially different ORs from ecosystem respiration fluxes and therefore should be better quantified to reduce uncertainties associated with our understanding of the global atmospheric carbon budget. Copyright 2009 by the American Geophysical Union.

Journal of Geophysical Research G: Biogeosciences↗

Sustaining healthy freshwater ecosystems

Functionally intact and biologically complex freshwater ecosystems provide many economically valuable commodities and services to society. The services supplied by freshwater ecosystems include flood control, transportation, recreation, purification of human and industrial wastes, habitat for plants and animals, and production of fish and other foods and marketable goods. These human benefits are called ecological services, defined as “the conditions and processes through which natural ecosystems, and the species that make them up, sustain and fulfill human life” (Daily 1997). Over the long term, healthy freshwater ecosystems are likely to retain the adaptive capacity to sustain production of these ecological services in the face of future environmental disruptions such as climate change.

Water Resources Update↗

Tamarisk (Tamarix spp.) water fluxes before, during and after episodic defoliation by the saltcedar leaf beetle

Tamarisk (Tamarix) species are among the most successful and economically costly plant invaders in the western United States, in part due to its potential to remove large amounts of water from shallow aquifers. Accordingly, local, state and federal agencies have released a new biological control - the saltcedar leaf beetle (Diorhabda elongata) along many watersheds in the western United States to reduce the spread of tamarisk. The beetle defoliates tamarisk for much of the growing season resulting in potentially large seasonal declines in productivity, fitness, and water loss from tamarisk stands. We measured sap flux density (J s ) using heat dissipation sensors to investigate water use patterns of tamarisk before, during and after a single, six week beetle-induced defoliation event in southeastern, Utah, USA. Granier-style probes were installed on 20 dominant trees from May through September 2008, a period that covers almost the entire growing season. As the beetle emerged from dormancy in mid-June, daytime and nighttime J s measurably increased for approximately two weeks before declining to less than 20% of predicted values (predicted by modeling J s with atmospheric vapor pressure deficit in May and June before defoliation). Tamarisk trees in mid-August produced new leaves and J s returned to pre-defoliation levels. Total J s , summed over the duration of the study was 13% lower than predicted values. These data suggest that defoliation results in only small changes in seasonal water loss from tamarisk stands. Current research is focusing on long-term ecohydrological impacts of tamarisk defoliation over multiple growing seasons.

Book↗

Measuring and interpreting the surface and shallow subsurface process influences on coastal wetland elevation: A review

A century ago, measuring elevation in tidal wetlands proved difficult, as survey leveling of soft marsh soils relative to a fixed datum was error prone. For 60 years, vertical accretion measures from marker horizons were used as analogs of elevation change. But without a direct measure of elevation, it was not possible to measure the total influence of surface and subsurface processes on elevation. In the 1990s, the surface elevation table (SET) method, which measures the movement of the wetland surface relative to a fixed point beneath the surface (i.e., the SET benchmark base), was combined with the marker horizon method (SET-MH), providing direct, independent, and simultaneous measures of surface accretion and elevation and quantification of surface and shallow subsurface process influences on elevation. SET-MH measures have revealed several fundamental findings about tidal wetland dynamics. First, accretion [ A ] is often a poor analog for elevation change [ E ]. From 50–66% of wetlands experience shallow subsidence ( A > E ), 7–10% shallow expansion ( A < E ), 7% shrink-swell, and for 24–36% A is an analog for E ( A = E ). Second, biological processes within the root zone and physical processes within and below the root zone influence elevation change in addition to surface processes. Third, vegetation plays a key role in wetland vertical dynamics. Plants trap sediment and increase resistance to erosion and compaction. Soil organic matter accumulation can lead to shallow expansion, but reduced plant growth can lead to subsidence, and plant death to soil collapse. Fourth, elevation rates are a better indicator of wetland response to sea-level rise than accretion rates because they incorporate subsurface influences on elevation occurring beneath the marker horizon. Fifth, combining elevation trends with relative sea-level rise (RSLR) trends improves estimates of RSLR at the wetland surface (i.e., RSLR wet ). Lastly, subsurface process influences are fundamental to a wetland’s response to RSLR and plant community dynamics related to wetland transgression, making the SET-MH method an invaluable tool for understanding coastal wetland elevation dynamics.

Estuaries and Coasts↗

Differentiating aquatic plant communities in a eutrophic river using hyperspectral and multispectral remote sensing

This study evaluates the efficacy of remote sensing technology to monitor species composition, areal extent and density of aquatic plants (macrophytes and filamentous algae) in impoundments where their presence may violate water-quality standards. Multispectral satellite (IKONOS) images and more than 500 in situ hyperspectral samples were acquired to map aquatic plant distributions. By analyzing field measurements, we created a library of hyperspectral signatures for a variety of aquatic plant species, associations and densities. We also used three vegetation indices. Normalized Difference Vegetation Index (NDVI), near-infrared (NIR)-Green Angle Index (NGAI) and normalized water absorption depth (DH), at wavelengths 554, 680, 820 and 977 nm to differentiate among aquatic plant species composition, areal density and thickness in cases where hyperspectral analysis yielded potentially ambiguous interpretations. We compared the NDVI derived from IKONOS imagery with the in situ, hyperspectral-derived NDVI. The IKONOS-based images were also compared to data obtained through routine visual observations. Our results confirmed that aquatic species composition alters spectral signatures and affects the accuracy of remote sensing of aquatic plant density. The results also demonstrated that the NGAI has apparent advantages in estimating density over the NDVI and the DH. In the feature space of the three indices, 3D scatter plot analysis revealed that hyperspectral data can differentiate several aquatic plant associations. High-resolution multispectral imagery provided useful information to distinguish among biophysical aquatic plant characteristics. Classification analysis indicated that using satellite imagery to assess Lemna coverage yielded an overall agreement of 79% with visual observations and >90% agreement for the densest aquatic plant coverages. Interpretation of biophysical parameters derived from high-resolution satellite or airborne imagery should prove to be a valuable approach for assessing the effectiveness of management practices for controlling aquatic plant growth in inland waters, as well as for routine monitoring of aquatic plants in lakes and suitable lentic environments. ?? 2010 Blackwell Publishing Ltd.

Freshwater Biology↗

Passive restoration of vegetation and biological soil crusts following 80 years of exclusion from grazing across the Great Basin

Restoration targets for biological soil crusts are largely unknown. We surveyed seven 80‐year‐old grazing exclosures across northern Nevada for biocrusts to quantify reference conditions at relatively undisturbed sites. Exclosures were associated with the following plant communities: Wyoming big sagebrush, black sagebrush, and areas co‐dominated by winterfat and Wyoming big sagebrush. Cover of biocrusts and shrubs were generally higher than other plant groups at these sites, regardless of being inside or outside of the exclosures, suggesting these groups make up most of the native flora across the region. Important in forming soil structure, cyanobacteria of the order Oscillatoriales were less abundant and diverse in black sagebrush communities. Grazing had a negative effect on the abundance of Oscillatoriales but not the number of algal taxa, including cyanobacteria. Abundance of light algal crusts were not influenced by plant community or grazing. Dark algal crusts were generally less abundant on grazed sites. Influences of plant community and grazing were most apparent when accounting for reproductive rates of lichens and mosses based on establishment mechanisms. Abundance of shrubs, perennial grasses, Oscillatoriales, fast reproducing biocrusts and the number of algal and cyanobacterial taxa, varied by plant community, suggesting that restoration should be plant community specific. We demonstrate the affinity of rapidly reproducing biocrusts for winterfat‐Wyoming big sagebrush co‐dominated plant communities, regardless of grazing pressure. Across sites, the effects of grazing were most evident on the abundance of Oscillatoriales and slowly reproducing biocrusts following 80 years of cessation from grazing.

Great Basin↗

Tamarisk beetle ( Diorhabda spp.) in the Colorado River basin: Synthesis of an expert panel forum

Executive Summary In 2001, the U.S. Department of Agriculture approved the release of a biological control agent, the tamarisk beetle ( Diorhabda spp.), to naturally control tamarisk populations and provide a less costly, and potentially more effective, means of removal compared with mechanical and chemical methods. The invasive plant tamarisk ( Tamarix spp.; saltcedar) occupies hundreds of thousands of acres of river floodplains and terraces across the western half of the North American continent. Its abundance varies, but can include dense monocultures, and can alter some physical and ecological processes associated with riparian ecosystems. The tamarisk beetle now occupies hundreds of miles of rivers throughout the Upper Colorado River Basin (UCRB) and is spreading into the Lower Basin. The efficacy of the beetle is evident, with many areas repeatedly experiencing tamarisk defoliation. While many welcome the beetle as a management tool, others are concerned by the ecosystem implications of widespread defoliation of a dominant woody species. As an example, defoliation may possibly affect the nesting success of the endangered southwestern willow flycatcher ( Empidonax traillii extimus ). In January 2015, the Tamarisk Coalition convened a panel of experts to discuss and present information on probable ecological trajectories in the face of widespread beetle presence and to consider opportunities for restoration and management of riparian systems in the Colorado River Basin (CRB). An in-depth description of the panel discussion follows.

Arizona, California, Chihuahua, Colorado, Idaho, K↗

Integrated assessment of chemical and biological recovery after diversion and treatment of acid mine drainage in a Rocky Mountain stream

Responses of stream ecosystems to gradual reductions in metal loading following remediation or restoration activities have been well documented in the literature. However, much less is known about how these systems respond to the immediate or more rapid elimination of metal inputs. Construction of a water treatment plant on the North Fork of Clear Creek (NFCC; CO, USA), a US Environmental Protection Agency Superfund site, captured, diverted, and treated the two major point-source inputs of acid mine drainage (AMD) and provided an opportunity to investigate immediate improvements in water quality. We conducted a 9-year study that included intensive within- and among-year monitoring of receiving-stream chemistry and benthic communities before and after construction of the treatment plant. Results showed a 64%–86% decrease in metal concentrations within months at the most contaminated sites. Benthic communities responded with increased abundance and diversity, but downstream stations remained impaired relative to reference conditions, with significantly lower taxonomic richness represented by a few dominant taxa (i.e., Baetis sp., Hydropsyche sp., Simulium sp., Orthocladiinae). Elevated metal concentrations from apparent residual sources, and relatively high conductivity from contributing major ions not removed during the treatment process, are likely limiting downstream recovery. Our study demonstrates that direct AMD treatment can rapidly improve water quality and benefit aquatic life, but effectiveness is limited, in part, to the extent that inputs of metals are captured and treated. Consideration should also be given to the effects of elevated major ion concentrations from the treated effluent not removed during the lime treatment process. Continued chemical and biological monitoring will be needed to quantify the NFCC recovery trajectory and to inform future remediation strategies.

Colorado↗

Nature reserves: Do they capture the full range of America's biological diversity?

Less than 6% of the coterminous United States is in nature reserves. Assessment of the occurrence of nature reserves across ranges of elevation and soil productivity classes indicates that nature reserves are most frequently found at higher elevations and on less productive soils. The distribution of plants and animals suggests that the greatest number of species is found at lower elevations. A preliminary assessment of the occurrence of mapped land cover types indicates that ???60% of mapped cover types have <10% of their area in nature reserves Land ownership patterns show that areas of lower elevation and more productive soils are most often privately owned and already extensively converted to urban and agricultural uses. Thus any effort to establish a system of nature reserves that captures the full geographical and ecological range of cover types and species must fully engage the private sector.

Ecological Applications↗

Anthropogenic tracers, endocrine disrupting chemicals, and endocrine disruption in Minnesota lakes

Concentrations of endocrine disrupting chemicals and endocrine disruption in fish were determined in 11 lakes across Minnesota that represent a range of trophic conditions and land uses (urban, agricultural, residential, and forested) and in which wastewater treatment plant discharges were absent. Water, sediment, and passive polar organic integrative samplers (POCIS) were analyzed for steroidal hormones, alkylphenols, bisphenol A, and other organic and inorganic molecular tracers to evaluate potential non-point source inputs into the lakes. Resident fish from the lakes were collected, and caged male fathead minnows were deployed to evaluate endocrine disruption, as indicated by the biological endpoints of plasma vitellogenin and gonadal histology. Endocrine disrupting chemicals, including bisphenol A, 17β-estradiol, estrone, and 4-nonylphenol were detected in 90% of the lakes at part per trillion concentrations. Endocrine disruption was observed in caged fathead minnows and resident fish in 90% of the lakes. The widespread but variable occurrence of anthropogenic chemicals in the lakes and endocrine disruption in fish indicates that potential sources are diverse, not limited to wastewater treatment plant discharges, and not entirely predictable based on trophic status and land use.

Minnesota↗

Natural attenuation can lead to environmental resilience in mine environment

Four streams flowing in the Iglesiente and Arburese mine districts (SW Sardinia, Italy), exploited for zinc (Zn) and lead (Pb) extraction from sulphides and secondary non-sulphide mineralization (calamine ores), have been studied combining investigations from the macroscale (hydrologic tracer techniques) to the microscale (X-ray powder diffraction, scanning electron microscopy, X-ray absorption spectroscopy). In the investigated area, concerns arise from release of metals to water during weathering of ore minerals and mine-waste. Specifically, Zn is observed at extremely high concentrations (10s of mg/L or more) in waters in some of the investigated catchments. The results from synoptic sampling campaigns showed marked differences of Zn loads, from 6.3 kg/day (Rio San Giorgio) to 2000 kg/day (Rio Irvi). Moreover, natural attenuation of metals was found to occur i) through precipitation of Fe compounds (Fe oxy/hydroxides and “green rust”), ii) by means of the authigenic formation of metal sulphides promoted by microbial sulphate reduction, iii) by metal intake in roots and stems of plants ( Phragmites australis and Juncus acutus ) and by immobilization in the rhizosphere, and iv) by cyanobacterial biomineralization processes that lead to formation of Zn-rich phases (hydrozincite and amorphous Zn-silicate) The biologically mediated natural processes that lead to significant abatement and/or reduction of metal loads, are the response of environmental systems to perturbations caused from mine activities, and can be considered part of the resilience of the system itself. The aim of this study is to understand the effect of these processes on the evolution of the studied systems towards more stable and, likely, resilient conditions, e.g. by limiting metal mobility and favoring the improvement of the overall quality of water. The understanding of how ecosystems adapt and respond to contamination, and which chemical and physical factors control these natural biogeochemical barriers, can help to plan effective remediation actions.

Sardinia↗

Signatures of adaptive divergence among populations of an avian species of conservation

Understanding the genetic underpinning of adaptive divergence among populations is a key goal of evolutionary biology and conservation. Gunnison sage‐grouse ( Centrocercus minimus ) is a sagebrush obligate species with a constricted range consisting of seven discrete populations, each with distinctly different habitat and climatic conditions. Though geographically close, populations have low levels of natural gene flow resulting in relatively high levels of differentiation. Here, we use 15,033 SNP loci in genomic outlier analyses, genotype–environment association analyses, and gene ontology enrichment tests to examine patterns of putatively adaptive genetic differentiation in an avian species of conservation concern. We found 411 loci within 5 kbp of 289 putative genes associated with biological functions or pathways that were overrepresented in the assemblage of outlier SNPs. The identified gene set was enriched for cytochrome P450 gene family members (CYP4V2, CYP2R1, CYP2C23B, CYP4B1) and could impact metabolism of plant secondary metabolites, a critical challenge for sagebrush obligates. Additionally, the gene set was also enriched with members potentially involved in antiviral response (DEAD box helicase gene family and SETX). Our results provide a first look at local adaption for isolated populations of a single species and suggest adaptive divergence in multiple metabolic and biochemical pathways may be occurring. This information can be useful in managing this species of conservation concern, for example, to identify unique populations to conserve, avoid translocation or release of individuals that may swamp locally adapted genetic diversity, or guide habitat restoration efforts.

Arizona, Colorado, New Mexico, Utah↗

Comparison of wetlands in different hydrogeological settings under conditions of extreme climate variability

Wetlands in the Cottonwood Lake area in North Dakota, USA, are underlain by poorly permeable till and have little groundwater input. Lakes and wetlands in the Shingobee River headwaters in Minnesota are underlain by permeable sand and have substantial groundwater input. Hydrological, chemical, and biological characteristics of these ecosystems have been monitored since 1977. Both sites experienced the second worst drought of the 20th century followed by the wettest period in more than a century. At Cottonwood Lake, plants that invaded the dry wetlands during the drought were flooded during the wet period and became a food source for animals. This resulted in successive substantial population increases and declines of plankton, invertebrates, amphibians and waterfowl. Substantial groundwater input buffered the lakes and wetlands in the Shingobee area against the changing water conditions. Only subtle changes in water chemistry and plankton populations were observed during the transition from drought to deluge.

IAHS-AISH Publication↗

Mathematical models for plant-herbivore interactions

Mathematical Models of Plant-Herbivore Interactions addresses mathematical models in the study of practical questions in ecology, particularly factors that affect herbivory, including plant defense, herbivore natural enemies, and adaptive herbivory, as well as the effects of these on plant community dynamics. The result of extensive research on the use of mathematical modeling to investigate the effects of plant defenses on plant-herbivore dynamics, this book describes a toxin-determined functional response model (TDFRM) that helps explains field observations of these interactions. This book is intended for graduate students and researchers interested in mathematical biology and ecology.

Book↗

Integrated assessment of wastewater reuse, exposure risk, and fish endocrine disruption in the Shenandoah River watershed

Reuse of municipal and industrial wastewater treatment plant (WWTP) effluent is an important component in augmenting global freshwater supplies. The Shenandoah River Watershed was selected to conduct on-site exposure experiments to assess endocrine disrupting characteristics of different source waters. This investigation of the Shenandoah River Watershed integrates WWTP wastewater reuse modeling, hydrological and chemical characterization, and in vivo endocrine disruption bioassessment to assess contaminant sources, exposure pathways, and biological effects. The percentage of accumulated WWTP effluent in each river reach (ACCWW) was used to predict environmental concentrations for consumer product chemicals (boron), pharmaceutical compounds (carbamazepine), and steroidal estrogens (estrone, 17-beta-estradiol, estriol, and 17-alpha-ethinylestradiol). Fish endocrine disruption was evaluated using vitellogenin induction in male or juvenile fathead minnows. Water samples were analyzed for >500 inorganic and organic constituents to characterize the complex contaminant mixtures. Municipal ACCWW at drinking water treatment plant surface-water intakes ranged from <0.01 to 2.1 % under mean-annual streamflow and up to 4.7 % under August streamflow. Measured and predicted environmental concentrations resulted in 17-beta-estradiol equivalency quotients ranging from <0.05 to 5.1 ng L-1 indicating low-to-moderate risk of fish endocrine disruption. Results from the fish exposure experiments also showed limited estrogenic effects as indicated by the low (0.5- to 3.2-fold) vitellogenin induction.

Shenandoah River watershed↗