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Fish and wildlife species as sentinels of environmental endocrine disruption

This chapter provides an overview of the history and criteria for use of captive and free-ranging fish and wildlife (amphibians, reptiles, birds, and mammals) species as sentinels of potential environmental endocrine disruption. Biochemical, behavioral, physiological, immunological, genetic, reproductive, developmental, and ecological correlates of endocrine disruption in these sentinels are presented and reviewed. In addition, data needs to promote better use of sentinel species in the assessment of endocrine disruption are discussed.

Book chapter

Pecos River Basin salinity assessment, Santa Rosa Lake, New Mexico, to the confluence of the Pecos River and the Rio Grande, Texas, 2015

The elevated salinity of the Pecos River throughout much of its length is of paramount concern to water users and water managers. Dissolved-solids concentrations in the Pecos River exceed 3,000 milligrams per liter in many of its reaches in the study area, from Santa Rosa Lake, New Mexico, to the confluence of the Pecos River with the Rio Grande, Texas. The salinity of the Pecos River increases downstream and affects the availability of useable water in the Pecos River Basin. In this report, “salinity” and “dissolved-solids concentration” are considered synonymous; both terms are used to refer to the total ionic concentration of dissolved minerals in water. The sources of salinity in the Pecos River Basin are natural (geologic) and anthropogenic, including but not limited to groundwater discharge, springs, and irrigation return flows. Previous studies in the Pecos River Basin were project specific and designed to address salinity issues in specific parts of the basin; therefore, in 2015, the U.S. Geological Survey in cooperation with the U.S. Army Corps of Engineers, New Mexico Interstate Stream Commission, Texas Commission on Environmental Quality, and Texas Water Development Board assessed the major sources of salinity throughout the extent of the basin where elevated salinity in the Pecos River is well documented (that is, in the drainage area of the Pecos River from Santa Rosa Lake to the confluence of the Pecos River and the Rio Grande). The goal was to gain a better understanding of how specific areas might be contributing to the elevated salinity in the Pecos River and how salinity of the Pecos River has changed over time. This assessment includes a literature review and compilation of previously published salinity-related data, which guided the collection of additional water-quality samples and streamflow gain-loss measurements. Differences in water quality of surface-water and groundwater samples, streamflow measurements, and geophysical data were assessed to gain new insights regarding sources of salinity in the Pecos River Basin and a more detailed assessment of potential areas of elevated salinity in the basin. The datasets compiled for this assessment are available in a companion data release. The literature review identified several potential sources of salinity inputs to the Pecos River in New Mexico and Texas. In New Mexico, sources of salinity inputs included sinkhole springs discharging into El Rito Creek, the Bitter Lake National Wildlife Refuge inflow to the Pecos River, inflow from the Rio Hondo, including the main channel and a restored channel at the Bitter Lake National Wildlife Refuge referred to as the “Rio Hondo spring channel,” the outflow from Lea Lake at Bottomless Lakes State Park, and the Malaga Bend region of the Pecos River. In Texas, sources of salinity inputs included Salt Creek downstream from Red Bluff Reservoir and the area near the Horsehead Crossing ford on the Pecos River. The compilation of historical water-quality data revealed a lack of consistent sampling of the same constituents at the same sites along the main stem of the Pecos River, which results in data gaps that hinder the ability to effectively analyze long-term changes in water quality that may help with the understanding of how salinity in the Pecos River has changed over time and identifying the sources of salinity in the Pecos River Basin. To help fill these data gaps, water-quality and streamflow data were collected in the study area in February 2015 by the U.S. Geological Survey. Historical water-quality data and newly collected data from February 2015 were evaluated for selected major-ion concentrations, dissolved-solids concentrations, and deuterium, oxygen, and strontium isotopes. Analysis of the data indicated several areas of increasing salinity in the Pecos River. Most notable increases were in two subreaches of the river, between Acme, N. Mex., and Artesia, N. Mex., and between Orla, Tex., and Grandfalls, Tex. Increasing sodium and chloride concentrations from Acme to Artesia coincided with changes in isotopic ratios within the Pecos River Basin. Changes in isotopic ratios in this reach indicate a likely inflow from an isotopically different source of water compared to the water in the main stem of the Pecos River, such as groundwater inflow, inflow from surface-water features distinct from the main stem of the Pecos River, or both. In the subreach between Orla and Grandfalls, an increase in dissolved-solids concentrations was observed along with a shift in isotope values, indicating that neither evaporative processes in Red Bluff Reservoir nor inflow from Salt Creek likely solely influences the salinity of the Pecos River in this subreach. The highest dissolved-solids concentrations in the Pecos River Basin were measured downstream from Grandfalls, where dissolved-solids concentrations are greater than 16,000 milligrams per liter near Iraan, Tex. Changes in isotopic values (deuterium, oxygen, and strontium) indicate mixing of different waters at several areas along the main stem of the Pecos River. The spatial distribution of the areas of interest from the literature review and the water-quality data are available in the companion data release.

Texas, New Mexico

Annotated bibliography of scientific research on greater sage-grouse published from October 2019 to July 2022

Integrating recent scientific knowledge into management decisions supports effective natural resource management and can lead to better resource outcomes. However, finding and accessing scientific knowledge can be time consuming and costly. To assist in this process, the U.S. Geological Survey (USGS) created a series of annotated bibliographies on topics of management concern for western lands. Previously published reports introduced a methodology for preparing annotated bibliographies to facilitate integration of recent, peer-reviewed science into resource management decisions. Therefore, relevant text from those efforts is reproduced here and built on to incorporate new information. The greater sage-grouse (Centrocercus urophasianus; hereafter “GRSG”) has been a focus of scientific investigation and management action for the past two decades. The 2015 U.S. Fish and Wildlife Service listing determination of “not warranted” under the Endangered Species Act was in part a result of a large-scale collaborative effort to develop strategies to conserve GRSG populations and their habitat and to reduce threats to both. New scientific information augments existing knowledge and can help inform updates or modifications to existing plans for managing GRSG and sagebrush ecosystems. However, the sheer number of scientific publications can be a challenge for managers tasked with evaluating and determining the need for potential updates to existing planning documents. To assist in this process, the USGS has reviewed and summarized the scientific literature published since January 1, 2015. Our most recent GRSG literature summary was published in 2020 (Carter and others, 2020) and included products published through October 2, 2019. Here, we consider products published between October 2, 2019, and July 21, 2022. We compiled and summarized peer-reviewed journal articles, data products, and formal technical reports (such as U.S. Department of Agriculture Forest Service General Technical Reports and USGS Open-File Reports) on greater sage-grouse. We first systematically searched three reference databases and three government databases using the search phrase “greater sage-grouse.” We refined the initial list of products by removing (1) duplicates, (2) publications not published as research, data products, or scientific review articles in peer-reviewed journals or as formal technical reports, and (3) products for which greater sage-grouse was not a research focus or the study did not present new data or findings about greater sage-grouse. We summarized each product using a consistent structure (background, objectives, methods, location, findings, and implications) and identified management topics addressed by each product; for example, species and population characteristics. We also identified projects that provided new geospatial data. The review process for this annotated bibliography included two initial internal colleague reviews of each summary, requesting input on each summary from an author of the original publication, and formal peer review. Our initial searches resulted in 221 total products, of which 147 met our criteria for inclusion. Across products summarized in the annotated bibliography, broad-scale habitat characteristics, behavior or demographics, site-scale habitat characteristics, habitat selection, and population estimates or targets were the most commonly addressed management topics. The online version of this bibliography, which will be available on the Science for Resource Managers tool ( https://apps.usgs.gov/science-for-resource-managers ), will be searchable by topic, location, and year, and will include links to each original publication. The studies compiled and summarized here may inform planning and management actions that seek to maintain and restore sagebrush landscapes and GRSG populations across the GRSG range.

Open-File Report

Site-characteristic and hydrologic data for selected wells and springs on Federal land in Clark County, Nevada

Site-characteristic and hydrologic data for selected wells and springs on U.S. Bureau of Land Management, National Park Service, U.S. Fish and Wildlife Service, and U.S. Forest Service land in Clark County, Nevada, were updated in the U.S. Geological Survey’s National Water Information System (NWIS) to facilitate multi-agency research. Data were researched and reviewed, sites were visited, and NWIS data were updated for 231 wells and 198 springs, including 36 wells and 67 springs that were added to NWIS and 44 duplicate sites that were deleted. The site-characteristic and hydrologic data collected, reviewed, edited, and added to NWIS include locations, well water levels, spring discharges, and water chemistry. Site-characteristic and hydrologic data can be accessed from links to the NWIS web interface; data not available through the web interface are presented in appendixes to this report.

Nevada

Most random-encounter-model density estimates in camera-based predator-prey studies are unreliable

Population estimates are often required for identifying relationships between predators and their prey and to inform conservation and management actions. The random encounter model (REM) estimates population density of wildlife lacking individually unique markings, based on photographs or videos from remote camera-traps. However, the REM has strict sampling and input requirements that can be problematic, particularly for predators and other species which use landscapes non-randomly. Using data from a predator and its co-occurring prey, we found that placing cameras to target the predator, which may be implemented to achieve minimum sample sizes, inflated both predator and prey density estimates. Further, borrowing movement velocity (day range) values from other studies, species, or time periods caused substantial changes in density estimates. A comprehensive literature review revealed that 91% of REM density estimates in published predator–prey studies used data from non-random cameras or borrowed movement velocities and therefore did not satisfy REM requirements. Consequently, most REM density estimates from predator–prey ecology studies are likely not of the quality or reliability necessary for informing effective wildlife conservation or management.

Nevada

Climate change effects on deer and moose in the midwest

Climate change is an increasing concern for wildlife managers across the United States and Canada. Because climate change may alter populations and harvest dynamics of key species in the region, midwestern states have identified the effects of climate change on ungulates as a priority research area. We conducted a literature review of projected climate change in the Midwest and the potential effects on white‐tailed deer ( Odocoileus virginianus ) and moose ( Alces alces ). Warmer temperatures and decreasing snowpack in the region favor survival of white‐tailed deer. In contrast, moose may become physiologically stressed in response to warming, and increasing deer populations spreading disease will exacerbate the problem. Although there is some uncertainty about exactly how the climate will change, and to what degree, robust projections suggest that deer populations will increase in response to climate change and moose populations will decrease. Managers can begin preparing for these changes by proactively creating management plans that take this into account.

Journal of Wildlife Management

Western bumble bee: Declines in United States and range-wide information gaps

In recent decades, many bumble bee species have declined due to changes in habitat, climate, and pressures from pathogens, pesticides, and introduced species. The western bumble bee ( Bombus occidentalis ), once common throughout western North America, is a species of concern and will be considered for listing by the U.S. Fish and Wildlife Service (USFWS) under the Endangered Species Act (ESA). We attempt to improve alignment of data collection and research with USFWS needs to consider redundancy, resiliency, and representation in the upcoming species status assessment. We reviewed existing data and literature on B. occidentalis , highlighting information gaps and priority topics for research. Priorities include increased knowledge of trends, basic information on several life‐history stages, and improved understanding of the relative and interacting effects of stressors on population trends, especially the effects of pathogens, pesticides, climate change, and habitat loss. An understanding of how and where geographic range extent has changed for the two subspecies of B. occidentalis is also needed. We outline data that could be easily collected in other research projects that would increase their utility for understanding range‐wide trends of bumble bees. We modeled the overall trend in occupancy from 1998 to 2018 of Bombus occidentalis within the continental United States using existing data. The probability of local occupancy declined by 93% over 21 yr from 0.81 (95% CRI = 0.43, 0.98) in 1998 to 0.06 (95% CRI = 0.02, 0.16) in 2018. The decline in occupancy varied spatially by landcover and other environmental factors. Detection rates vary in both space and time, but peak detection across the continental United States occurs in mid‐July. We found considerable spatial gaps in recent sampling, with limited sampling in many regions, including most of Alaska, northwestern Canada, and the southwestern United States. We therefore propose a sampling design to address these gaps to best inform the ESA species status assessment through improved assessment of how the spatial distribution of stressors influences occupancy changes. Finally, we request involvement via data sharing, participation in occupancy sampling with repeated visits to distributed survey sites, and complementary research to address priorities outlined in this paper.

Ecosphere

General and specialized media routinely employed for primary isolation of bacterial pathogens of fishes

There are a number of significant diseases among cultured and free-ranging freshwater fishes that have a bacterial etiology; these represent a variety of gram-negative and gram-positive genera. Confirmatory diagnosis of these diseases involves primary isolation of the causative bacterium on bacteriologic media. Frequently used "general" bacteriologic media simply provide the essential nutrients for growth. For most of the major pathogens, however, there are differential and/or selective media that facilitate primary recovery. Some specialized media are available as "ready-to-use" from suppliers, while others must be prepared. Differential media employ various types of indicator systems, such as pH indicators, that allow diagnosticians to observe assimilation of selected substrates. An advantage to the use of differential media for primary isolation is that they hasten bacterial characterization by yielding the appropriate positive or negative result for a particular substrate, often leading to a presumptive identification. Selective media also incorporate agent(s) that inhibit the growth of contaminants typically encountered with samples from aquatic environments. Media that incorporate differential and/or selective components are ideally based on characters that are unique to the targeted bacterium, and their use can reduce the time associated with diagnosis and facilitate early intervention in affected fish populations. In this review, the concepts of general and differential/selective bacteriologic media and their use and development for fish pathogens are discussed. The media routinely employed for primary isolation of the significant bacterial pathogens of fishes are presented. ?? Wildlife Disease Association 2008.

Journal of Wildlife Diseases

Balancing the scales: Including under-represented herptile species in a One Health approach

The One Health High-Level Expert Panel’s definition of One Health includes optimizing the health of people, animals (wild and domestic) and ecosystems. For many One Health practitioners, wildlife that can spread zoonoses are the focus, particularly if they can come in contact with people. However, ecosystem health is often best-indicated by less-encountered species, for instance, amphibians and reptiles. This review highlights how these taxa can benefit human health and well-being, including cultural significance, as well as their impact on plant, animal and environmental health. We highlight current challenges to the health of these species and the need to include them in the One Health Joint Action Plan. We conclude with a call to action for inclusion of amphibians and reptiles in a One Health approach.

Research Directions: One Health

Pacific Lamprey responses to stressors: Dewatering and electrofishing

The Pacific Lamprey Conservation Initiative (PLCI) is a collaboration of Tribes, Federal, and State agencies working together to protect and restore Pacific Lamprey ( Entosphenus tridentatus ) and other native lampreys (i.e., Lampetra spp.) in Alaska, Washington, Oregon, California, and Idaho. The U.S. Fish and Wildlife Service hosts and facilitates the PLCI, and the Columbia River Tribes play a large role in setting conservation goals and defining research needs. The PLCI annually solicits proposals for research and restoration activities, which are reviewed and ranked in collaboration with the Bonneville Power Administration (BPA), who annually provides funding to support PLCI priority proposals. This report summarizes two research projects selected through PLCI, and funded under one BPA contract, in support of Pacific Lamprey conservation. The two projects were not topically related apart from a common theme of potential stressors to lampreys and are being reported together because they were combined under one BPA agreement for contracting.

Report

Integrating climate-informed planning into State Wildlife Action Plans in the north central United States

State fish and wildlife agencies are required to submit a State Wildlife Action Plan (SWAP) every 10 years to be eligible for grants through the State Wildlife Grant Program. With the next round of revisions due in 2025, the U.S. Geological Survey North Central Climate Adaptation Science Center is evaluating how to best support states with further integrating climate-informed planning in their SWAPs. Here, we summarize how states in the North Central region, which includes Colorado, Kansas, Montana, Nebraska, North Dakota, South Dakota, and Wyoming, addressed adaptation planning in their 2015 SWAPs. We review climate adaptation considerations in required SWAP elements one through five, including species, habitats, threats, conservation actions, and conservation monitoring, respectively. We also include a list of potential strategies that the North Central Climate Adaptation Science Center could use to support states in the North Central region with further advancing the integration of climate informed planning in ongoing and future SWAP revisions. These strategies were primarily identified during meetings in 2021 with six of seven state fish and wildlife agencies in the North Central region where we discussed their priority needs relating to climate-informed planning. Our hope is that these strategies will enhance existing efforts in the region to advance the integration of climate change in conservation planning.

Colorado, Kansas, Montana, Nebraska, North Dakota,

Errors in aerial survey count data: Identifying pitfalls and solutions

Accurate estimates of animal abundance are essential for guiding effective management, and poor survey data can produce misleading inferences. Aerial surveys are an efficient survey platform, capable of collecting wildlife data across large spatial extents in short timeframes. However, these surveys can yield unreliable data if not carefully executed. Despite a long history of aerial survey use in ecological research, problems common to aerial surveys have not yet been adequately resolved. Through an extensive review of the aerial survey literature over the last 50 years, we evaluated how common problems encountered in the data (including nondetection, counting error, and species misidentification) can manifest, the potential difficulties conferred, and the history of how these challenges have been addressed. Additionally, we used a double-observer case study focused on waterbird data collected via aerial surveys and an online group (flock) counting quiz to explore the potential extent of each challenge and possible resolutions. We found that nearly three quarters of the aerial survey methodology literature focused on accounting for nondetection errors, while issues of counting error and misidentification were less commonly addressed. Through our case study, we demonstrated how these challenges can prove problematic by detailing the extent and magnitude of potential errors. Using our online quiz, we showed that aerial observers typically undercount group size and that the magnitude of counting errors increases with group size. Our results illustrate how each issue can act to bias inferences, highlighting the importance of considering individual methods for mitigating potential problems separately during survey design and analysis. We synthesized the information gained from our analyses to evaluate strategies for overcoming the challenges of using aerial survey data to estimate wildlife abundance, such as digital data collection methods, pooling species records by family, and ordinal modeling using binned data. Recognizing conditions that can lead to data collection errors and having reasonable solutions for addressing errors can allow researchers to allocate resources effectively to mitigate the most significant challenges for obtaining reliable aerial survey data.

Alabama, Florida, Louisiana, Mississippi, Texas

Development of an IBI-based assessment of depressional wetlands in Maryland and Delaware

The hydrogeomorphic approach (HGM) of wetland assessment emphasizes functional components of wetlands such as water storage, transformation and cycling of elements, accumulation of sediments, and preservation of habitats. Many of the elements measured in HGM are physical rather than ecological or biological. The HGM approach, therefore, provides information on certain aspects of wetlands and omits other aspects. In contrast, the Index of Biological Integrity (IBI) approach focuses on biological components of wetlands such as species richness, the presence or proportion of certain 'indicator' species, representation of different trophic levels, and measures of wildlife or fish health. Here too, some aspects of a wetland are omitted and others not covered by HGM are included. We contend that these differences in focus add strengths and weaknesses to each method. This paper reviews progress on the development of IBIs for restored depressional wetlands in the Mid-Atlantic States, especially Maryland and Delaware. During our first field season we identified 25 wetlands ranging from 1-10 acres, most of which were restored with federal, state, and private landowner cooperation over hydric soils. Separate IBIs are being created for macrophytes, macroinvertebrates, amphibians, and data on mammal and avian populations are being collected. Simultaneously, chemical and physical data are being collected on water DO, turbidity, temperature, conductivity, nitrates, ammonia, chlorophyll, pH; soil metal levels and texture; and wetland size, configuration, hydrology, drainage area, and surrounding land use.

Book chapter

Enhanced surveillance strategies for detecting and monitoring chronic wasting disease in free-ranging cervids

The purpose of this document is to provide wildlife management agencies with the foundation upon which they can build scientifically rigorous and cost-effective surveillance and monitoring programs for chronic wasting disease (CWD) or refine their existing programs. The first chapter provides an overview of potential demographic and spatial risk factors of susceptible wildlife populations that may be exploited for CWD surveillance and monitoring. The information contained in this chapter explores historic as well as recent developments in our understanding of CWD disease dynamics. It also contains many literature references for readers who may desire a more thorough review of the topics or CWD in general. The second chapter examines methods for enhancing efforts to detect CWD on the landscape where it is not presently known to exist and focuses on the efficiency and cost-effectiveness of the surveillance program. Specifically, it describes the means of exploiting current knowledge of demographic and spatial risk factors, as described in the first chapter, through a two-stage surveillance scheme that utilizes traditional design-based sampling approaches and novel statistical methods to incorporate information about the attributes of the landscape, environment, populations and individual animals into CWD surveillance activities. By accounting for these attributes, efficiencies can be gained and cost-savings can be realized. The final chapter is unique in relation to the first two chapters. Its focus is on designing programs to monitor CWD once it is discovered within a jurisdiction. Unlike the prior chapters that are more detailed or prescriptive, this chapter by design is considerably more general because providing comprehensive direction for creating monitoring programs for jurisdictions without consideration of their monitoring goals, sociopolitical constraints, or their biological systems, is not possible. Therefore, the authors draw upon their collective experiences implementing disease-monitoring programs to present the important questions to consider, potential tools, and various strategies for those wildlife management agencies endeavoring to create or maintain a CWD monitoring program. Its intent is to aid readers in creating efficient and cost-effective monitoring programs, while avoiding potential pitfalls. It is hoped that these three chapters will be useful tools for wildlife managers struggling to implement efficient and effective CWD disease management programs.

Open-File Report

Benefits and impacts of road removal

Road removal is being used to mitigate the physical and ecological impacts of roads and to restore both public and private lands. Although many federal and state agencies and private landowners have created protocols for road removal and priorities for restoration, research has not kept pace with the rate of removal. Some research has been conducted on hydrologic and geomorphic restoration following road removal, but no studies have directly addressed restoring wildlife habitat. Road removal creates a short-term disturbance which may temporarily increase sediment loss. However, long-term monitoring and initial research have shown that road removal reduces chronic erosion and the risk of landslides. We review the hydrologic, geomorphic, and ecological benefits and impacts of three methods of road removal, identify knowledge gaps, and propose questions for future research, which is urgently needed to quantify how effectively road removal restores terrestrial, riparian, and aquatic habitat and other ecosystem processes.

Frontiers in Ecology and the Environment

Genetic conservation and paddlefish propagation

The conservation of genetic diversity of our natural resources is overwhelmingly one of the central foci of 21st century management practices. Three recommendations related to the conservation of paddlefish Polyodon spathula genetic diversity are to (1) identify genetic diversity at both nuclear and mitochondrial DNA loci using a suggested list of 20 sampling locations, (2) use genetic diversity estimates to develop genetic management units, and (3) identify broodstock sources to minimize effects of supplemental stocking on the genetic integrity of native paddlefish populations. We review previous genetic work on paddlefish and described key principles and concepts associated with maintaining genetic diversity within and among paddlefish populations and also present a genetic case study of current paddlefish propagation at the U.S. Fish and Wildlife Service Gavins Point National Fish Hatchery. This study confirmed that three potential sources of broodfish were genetically indistinguishable at the loci examined, allowing the management agencies cooperating on this program flexibility in sampling gametes. This study also showed significant bias in the hatchery occurred in terms of male reproductive contribution, which resulted in a shift in the genetic diversity of progeny compared to the broodfish. This shift was shown to result from differential male contributions, partially attributed to the mode of egg fertilization. Genetic insights enable implementation of a paddlefish propagation program within an adaptive management strategy that conserves inherent genetic diversity while achieving demographic goals.

American Fisheries Society Symposium

More than a decade of conservation biology and research in Sonora and Arizona: The endangered Sonoran Tiger Salamander and threatened Chiricahua Leopard Frog

Only two species of amphibians from Arizona are listed under the federal Endangered Species Act. The Sonoran Tiger Salamander was listed as an endangered species without critical habitat in 1997. The Chiricahua Leopard Frog was listed as a threatened species in 2002; critical habitat was designated in 2012. In this review, we summarize conservation and research conducted on these two species with a focus on work in the southeastern Arizona/northeastern Sonora borderlands, but we also touch upon seminal work on the Chiricahua Leopard Frog at Buenos Aires National Wildlife Refuge (BANWR).

Arizona, Sonora