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Finding a GEM: The Grassland Effectiveness Monitoring (GEM) protocol provides a tiered approach for habitat treatment assessment across private lands incentive programs

The soil store carbon dioxide from the atmosphere through different processes, depending on the use and management of the land. For the Maderas del Carmen and Ocampo natural protected areas, one of the main conservation goals is to preserve natural habitats, ensuring an ecological balance and making a sustainable. The main productive activity in the region is extensive livestock. Different strategies have been explored for producers to modify their practices to ensure sustainable management. In this study, we established the baseline of sustainable management indicators according to the sustainable management protocol. Soils showed a low activity and low C with a high bulk density. Establishing this baseline will allow a starting point for a long-term evaluation different from those traditionally used when evaluating the productivity of the rangeland.

Conference Paper

Physiological Ecology and Ecohydrology of Coastal Forested Wetlands

The form, function, and productivity of wetland communities are influenced strongly by the hydrologic regime of an area. Wetland ecosystems persist by depending upon surpluses of rainfall, evapotranspiration, soil moisture, and frequency and amplitude of water-level fluctuations. Yet, wetland vegetation can also influence ecosystem water economy through conservative water- and carbon-use strategies at several organizational scales. Scientists have described leaf-level water-use efficiency in coastal mangrove forests as being among the highest of any ecosystem. These forested wetlands occur in intertidal areas and often persist under flooded saline conditions. Are these same strategies used by other types of coastal forested wetlands? Do conservative water-use strategies reflect a consequence of salt balance more than efficiency in water use per se? At what organizational scales do these strategies manifest? These are just a few of the questions being answered by physiological and landscape ecologists at the U.S. Geological Survey National Wetlands Research Center (NWRC).

Fact Sheet

PRMS-IV, the precipitation-runoff modeling system, version 4

Computer models that simulate the hydrologic cycle at a watershed scale facilitate assessment of variability in climate, biota, geology, and human activities on water availability and flow. This report describes an updated version of the Precipitation-Runoff Modeling System. The Precipitation-Runoff Modeling System is a deterministic, distributed-parameter, physical-process-based modeling system developed to evaluate the response of various combinations of climate and land use on streamflow and general watershed hydrology. Several new model components were developed, and all existing components were updated, to enhance performance and supportability. This report describes the history, application, concepts, organization, and mathematical formulation of the Precipitation-Runoff Modeling System and its model components. This updated version provides improvements in (1) system flexibility for integrated science, (2) verification of conservation of water during simulation, (3) methods for spatial distribution of climate boundary conditions, and (4) methods for simulation of soil-water flow and storage.

Techniques and Methods

Season of grazing interacts with soil texture, selecting for associations of biocrust morphogroups

Livestock grazing, a widespread land use in semi-arid systems, is often placed in opposition to the perpetuation of biological soil crusts (“biocrusts”: lichens, mosses, and algal crusts including cyanobacteria) that live on the soil surface and provide ecosystem functions. The composition of biocrusts and vascular plants varies with climate, soils, and disturbance. In general, ruderal mosses and light algal crusts make up greater proportions of biocrusts in the presence of disturbance, although morphogroups of biocrusts respond differently to various disturbances. It is unknown if there are scenarios under which grazing can occur and ruderal components of biocrust could be maintained. We examine the hypothesis that soil surface texture-moisture interactions influence the ability of biocrusts to withstand trampling, reasoning that finer-textured soils are firmer (therefore serving as a better substrate for biocrusts) when dry and that coarser-textured are firmer when wet. We test these relationships within Birds of Prey, National Conservation Area (Boise, Idaho, USA). Results demonstrate two associations of biocrusts, dependent on season of grazing: one dominated by light algal crusts and lichens that frequently occurs with wet season grazing, and a second dominated by tall mosses and cup lichens that frequently occurs with dry season grazing. High cover of the invasive annual grass, Bromus tectorum (L.) was observed on sites with coarse-textured soils, and high sand content, that are grazed at relatively high intensities, creating unstable surfaces, and likely putting biocrusts at greater susceptibility to trampling. Results suggest that livestock management that accounts for soil texture and moisture could be used to maintain cover of ruderal biocrusts on fine-textured soils, that are grazed in the dry season, at low intensity. We discuss our findings in the context of managing for species of interest. Our findings are timely as varying the season of grazing is increasingly discussed as a means of favoring desirable native perennial grasses. Although ruderal morphogroups of biocrusts are not interpreted as having equivalent ecosystem functions compared to intact biocrusts, their contributions to soil stability, fertility, hydrology, and weed abatement could increase if they were more intentionally targeted by management.

Geoderma

Soil characteristics are associated with gradients of big sagebrush canopy structure after disturbance

Reestablishing shrub canopy cover after disturbance in semi-arid ecosystems, such as sagebrush steppe, is essential to provide wildlife habitat and restore ecosystem functioning. While several studies have explored the effects of landscape and climate factors on the success or failure of sagebrush seeding, the influence of soil properties on gradients of shrub canopy structure in successfully seeded areas remains largely unexplored. In this study, we evaluated associations between soil properties and gradients in sagebrush canopy structure in stands that had successfully reestablished after fire and subsequent seeding treatments. Using a dataset collected across the Great Basin, USA, of sagebrush stands that had burned and reestablished between 1986 and 2013, we tested soil depth and texture, soil surface classification, biological soil crusts plus mean historical precipitation, solar heatload, and fire history as modeling variables to explore gradients in sagebrush canopy structure growth in terms of cover, height, and density. Deeper soils were associated with greater sagebrush canopy structure development in terms of plant density and percent cover, coarser textured soils were associated with greater sagebrush cover and density, and more clayey soils were typically associated with greater height. Biological crust presence was also positively associated with enhanced sagebrush canopy growth, but adding more demographically or morphologically explicit descriptions of biocrust communities did not improve explanatory power. Increasing heatload had a negative effect on sagebrush canopy structure growth, and increased mean annual precipitation was only associated with greater sagebrush height. Given that conservation and restoration of the sagebrush steppe ecosystems has become a priority for land managers, the associations we identify between gradients in post-fire sagebrush canopy structure growth and field-identifiable soil characteristics may improve planning of land treatments for sagebrush restoration and the understanding of semi-arid ecosystem functioning and post-disturbance dynamics.

Ecosphere

Surface disturbance of cryptobiotic soil crusts: nitrogenase activity, chlorophyll content, and chlorophyll degradation

Cryptobiotic soil crusts are an important component of semiarid and arid ecosystems. An important role of these crusts is the contribution of fixed nitrogen to cold‐desert ecosystems. This study examines the residual effects of various intensities and combinations of different surface disturbances (raking, scalping, and tracked vehicles) on nitrogenase activity, chlorophyll content, and chlorophyll degradation in these soil crusts. Nine months after disturbance chlorophyll content of disturbed soils was not statistically different from undisturbed controls, except in the scalped treatments, indicating recovery of this characteristic is fairly quick unless surface material is removed. Differences in chlorophyll degradation among treatments were not statistically significant. However, nitrogenase activity in all treatments showed tremendous reductions, ranging from 77–97%, when compared to the control, indicating this characteristic is slow to recover. Consequently, assessment of crustal recovery from disturbance must include not only visual and biomass characteristics but other physiological measurements as well. Areas dominated by these crusts should be managed conservatively until the implications of crustal disturbance is better understood.

Arid Soil Research and Rehabilitation

Riparian vegetation dynamics and evapotranspiration in the riparian corridor in the delta of the Colorado River, Mexico

Like other great desert rivers, the Colorado River in the United States and Mexico is highly regulated to provide water for human use. No water is officially allotted to support the natural ecosystems in the delta of the river in Mexico. However, precipitation is inherently variable in this watershed, and from 1981–2004, 15% of the mean annual flow of the Lower Colorado River has entered the riparian corridor below the last diversion point for water in Mexico. These flows include flood releases from US dams and much smaller administrative spills released back to the river from irrigators in the US and Mexico. These flows have germinated new cohorts of native cottonwood and willow trees and have established an active aquatic ecosystem in the riparian corridor in Mexico. We used ground and remote-sensing methods to determine the composition and fractional cover of the vegetation in the riparian corridor, its annual water consumption, and the sources of water that support the ecosystem. The study covered the period 2000–2004, a flood year followed by 4 dry years. The riparian corridor occupies 30,000 ha between flood control levees in Mexico. Annual evapotranspiration (ET), estimated by Moderate Resolution Imaging Spectrometer (MODIS) satellite imagery calibrated against moisture flux tower data, was about 1.1 m yr −1 and was fairly constant throughout the study period despite a paucity of surface flows 2001–2004. Total ET averaged 3.4×10 8 m 3 yr −1 , about 15% of Colorado River water entering Mexico from the US Surface flows could have played only a small part in supporting these high ET losses. We conclude that the riparian ET is supported mainly by the shallow regional aquifer, derived from agricultural return flows, that approaches the surface in the riparian zone. Nevertheless, surface flows are important in germinating cohorts of native trees, in washing salts from the soil and aquifer, and in providing aquatic habitat, thereby enriching the habitat value of the riparian corridor for birds and other wildlife. Conservation and water management strategies to enhance the delta habitats are discussed in light of the findings.

Journal of Environmental Management

Monitoring the southwestern Wyoming landscape—A foundation for management and science

Natural resource monitoring involves repeated collections of resource condition data and analyses to detect possible changes and identify underlying causes of changes. For natural resource agencies, monitoring provides the foundation for management and science. Specifically, analyses of monitoring data allow managers to better understand effects of land-use and other changes on important natural resources and to achieve their conservation and management goals. Examples of natural resources monitored on public lands include wildlife habitats, plant productivity, animal movements and population trends, soil chemistry, and water quality and quantity. Broader definitions of monitoring also recognize the need for scientifically valid data to help support planning efforts and informed decisions, to develop adaptive management strategies, and to provide the means for evaluating management outcomes.

Wyoming

A compositional multiphase model for groundwater contamination by petroleum products: 1. Theoretical considerations

A mathematical model is developed to describe the fate of hydrocarbon constituents of petroleum products introduced to soils as an immiscible liquid from sources such as leaking underground storage tanks and ruptured pipelines. The problem is one of multiphase transport (oil (immiscible), air, and water phases) of a reactive contaminant with constituents such as benzene, toluene, and xylene found in refined petroleum products like gasoline. In the unsaturated zone, transport of each constituent can occur as a solute in the water phase, vapor in the air phase, and as an unaltered constituent in the oil phase. Additionally, the model allows for adsorption. Molecular transformations, microbially mediated or abiotic, are incorporated as sink terms in the conservation of mass equations. An equilibrium approximation, applicable to any immiscible organic contaminant is applied to partition constituent mass between the air, oil, water, and adsorbed phases for points in the region where the oil phase exists. Outside the oil plume the equilibrium approximation takes on a simpler form to partition constituent mass between the air, water, and adsorbed phases only. Microbial degradation of petroleum products is first discussed in a general model, then the conservation of mass equation for oxygen is incorporated into the analysis which takes advantage of the key role played by oxygen in the metabolism of hydrocarbon utilizing microbes in soil environments. Approximations to two subproblems, oil plume establishment in the unsaturated zone, and solute and vapor transport subsequent to immiscible plume establishment are then developed from the general model.

Water Resources Research

Understanding conservation decisions of agriculture producers

Most land in the United States (US) is privately owned and used for agriculture. To address the effect of agriculture on wildlife, conservation professionals and organizations need to understand the land use decisions made by farmers and ranchers. We developed a tool for categorizing farmers and ranchers by their conservation land use values (LUVs) to understand how those values affect their land use motivations and resultant decisions. We defined land as the whole natural environment including soil, water, plants, fish, and wildlife. We used principal axis factoring and reliability analysis to identify statements representing human-centered values and nature-centered values of farmers and ranchers. We tested the validity of the combined statements with a survey of South Dakota’s private landowners (N = 4,000, [Jan through May 2016]) resulting in the LUV scale. Crossing the average scores on the human-centered and nature-centered statements identified 4 LUV types: humans first (20%), nature first (29%), interconnected (29%), and disconnected (22%). Analysis of variance and chi-square tests showed that, compared to the humans first and disconnected LUV types, the nature first and interconnected LUV types reported significantly greater importance of the following: most categories of types of wildlife in their land use decisions; conservation-related motivations for participating in a United States Farm Bill Conservation Program; conservation-related motivations for land use decisions; and participation in conservation-related behaviors. Conservation professionals and organizations may use the LUV scale to better understand landowners’ land use decisions to evaluate and inform conservation policy, programs, messaging, and improve conservation outcomes.

Journal of Wildlife Management

Assessment of Hyporheic Zone, Flood-Plain, Soil-Gas, Soil, and Surface-Water Contamination at the McCoys Creek Chemical Training Area, Fort Gordon, Georgia, 2009-2010

The U.S. Geological Survey, in cooperation with the U.S. Department of the Army Environmental and Natural Resources Management Office of the U.S. Army Signal Center and Fort Gordon, Georgia, assessed the hyporheic zone, flood plain, soil gas, soil, and surface water for contaminants at the McCoys Creek Chemical Training Area (MCTA) at Fort Gordon, from October 2009 to September 2010. The assessment included the detection of organic contaminants in the hyporheic zone, flood plain, soil gas, and surface water. In addition, the organic contaminant assessment included the analysis of organic compounds classified as explosives and chemical agents in selected areas. Inorganic contaminants were assessed in soil and surface-water samples. The assessment was conducted to provide environmental contamination data to the U.S. Army at Fort Gordon pursuant to requirements of the Resource Conservation and Recovery Act Part B Hazardous Waste Permit process. Ten passive samplers were deployed in the hyporheic zone and flood plain, and total petroleum hydrocarbons (TPH) and octane were detected above the method detection level in every sampler. Other organic compounds detected above the method detection level in the hyporheic zone and flood-plain samplers were trichloroethylene, and cis- and trans- 1, 2-dichloroethylene. One trip blank detected TPH below the method detection level but above the nondetection level. The concentrations of TPH in the samplers were many times greater than the concentrations detected in the blank; therefore, all other TPH concentrations detected are considered to represent environmental conditions. Seventy-one soil-gas samplers were deployed in a grid pattern across the MCTA. Three trip blanks and three method blanks were used and not deployed, and TPH was detected above the method detection level in two trip blanks and one method blank. Detection of TPH was observed at all 71 samplers, but because TPH was detected in the trip and method blanks, TPH was censored and, therefore, only 7 of the 71 samplers were reported as detecting TPH. In addition, benzene, toluene, ethylbenzene, and total xylene were detected above the method detection level in 22 samplers. Other compounds detected above the method detection level included naphthalene, octane, undecane, tridecane, 1,2,4-trimethylbenzene, trichloroethylene, perchloroethylene, chloroform, and 1,4-dichlorobenzene. Subsequent to the soil-gas survey, five locations with elevated contaminant mass were selected and a passive sampler was deployed at those locations to detect the presence of organic compounds classified as explosives or chemical agents. No explosives or chemical agents were detected above the method detection level, but some compounds were detected below the method detection level but above the nondetection level. Dimethyl disulfide, benzothiazole, chloroacetophenones, and para-chlorophenyl methyl sulfide were all detected below the method detection level but above the nondetection level. The compounds 2,4-dinitrotoluene, and para-chlorophenyl methyl sulfone were detected in samplers but also were detected in trip blanks and are not considered as present in the MCTA. The same five locations that were selected for sampling of explosives and chemical agents were selected for soil sampling. Metal concentrations in composite soil samples collected at five locations from land surface to a depth of 6 inches did not exceed the U.S. Environmental Protection Agency Regional Screening Levels for Industrial Soil. Concentrations in some compounds were higher than the South Carolina Department of Health and Environmental Control background levels for nearby South Carolina, including aluminum, arsenic, barium, beryllium, chromium, copper, iron, lead, manganese, nickel, and potassium. A surface-water sample was collected from McCoys Creek and analyzed for volatile organic compounds, semivolatile organic compounds, and inorganic compounds (metals). No volatile organic compounds and (or) semivolatile organic compounds were detected at levels above the maximum contaminant level of the U.S. Environmental Protection Agency (USEPA) National Primary Drinking Water Standard, and no inorganic compounds exceeded the maximum contaminant level of the USEPA National Primary Drinking Water Standard or the Georgia In-Stream Water-Quality Standard. Iron was the only inorganic compound detected in the surface-water sample (578 micrograms per liter) that exceeded the USEPA National Secondary Drinking Water Standard of 300 micrograms per liter.

Georgia

The evolution of a colluvial hollow to a fluvial channel with periodic steps following two transformational disturbances: A wildfire and a historic flood

The transition of a colluvial hollow to a fluvial channel with discrete steps was observed after two landscape-scale disturbances. The first disturbance, a high-severity wildfire, changed the catchment hydrology to favor overland flow, which incised a colluvial hollow, creating a channel in the same location. This incised channel became armored with cobbles and boulders following repeated post-wildfire overland flow events. Three years after the fire, a record rainstorm produced regional flooding and generated sufficient fluvial erosion and sorting to produce a fluvial channel with periodically spaced steps. An analysis of the step spacing shows that after the flood, newly formed steps retained a similar spacing to the topographic roughness spacing in the original colluvial hollow (prior to channelization). This suggests that despite a distinct change in channel form roughness and bedform morphology, the endogenous roughness periodicity was conserved. Variations in sediment erodibility helped to create the emergent steps as the largest particles ( >D84) remained immobile, becoming step features, and downstream soil was easily winnowed away.

Geomorphology

Post-fire sediment yield from a western Sierra Nevada watershed burned by the 2021 Caldor Fire

Watershed sediment yield commonly increases after wildfire, often causing negative impacts to downstream infrastructure and water resources. Post-fire erosion is important to understand and quantify because it is increasingly placing water supplies, habitat, communities, and infrastructure at risk as fire regimes intensify in a warming climate. However, measurements of post-fire sediment mobilization are lacking from many regions. We measured sediment yield from a forested, heavily managed 25.4-km 2 watershed in the western Sierra Nevada, California, over 2 years following the 2021 Caldor Fire, by repeat mapping of a reservoir where sediment accumulated from terrain with moderate to high soil burn severity. Sediment yield was less than the geochronology-derived long-term average in the first year post-fire (conservatively estimated at 21.8–28.0 t/km 2 ), low enough to be difficult to measure with uncrewed airborne system (UAS) and bathymetric sonar survey methods that are most effective at detecting larger sedimentary signals. In the second year post-fire the sediment delivery was 1,560–2,010 t/km 2 , an order of magnitude above long-term values, attributable to greater precipitation and intensive salvage logging. Hillslope erosion simulated by the Water Erosion Prediction Project (WEPP) model overestimated the measured amount by a factor of 90 in the first year and in the second year by a factor (1.9) that aligned with previously determined model performance in northern California. We encourage additional field studies, and validation of erosion models where feasible, to further expand the range of conditions informing post-fire hazard assessments and management decisions.

California

Seasonally dynamic nutrient modeling quantifies storage lags and time-varying reactivity across large river basins

Nutrients that have gradually accumulated in soils, groundwaters, and river sediments in the United States over the past century can remobilize and increase current downstream loading, obscuring effects of conservation practices aimed at protecting water resources. Drivers of storage accumulation and release of nutrients are poorly understood at the spatial scale of basins to watersheds. Predicting water quality outcomes in large river basins demands modeling storage lags and time varying reactivity that models of mean conditions typically cannot elucidate. We developed a seasonally dynamic approach to large-scale nutrient modeling based on a multiscale framework and nutrient storage lags were quantified for the nearly 190 000 small catchments that feed the rivers across the northeastern United States where catchment mean transit times were found to be around 4.7 (2–10) years for nitrogen and 1.3 (0.7–2) years for phosphorus. Nutrient loads carried in river flow in the current season contained a significant—and sometimes dominant—portion of mass lagged in its release from catchment storage repositories. Our approach of integrating storage releases with seasonally dynamic hydroclimatic drivers sets the stage to assess the accumulated effects of nutrient storage and lagged releases to the river interacting with seasonally varying nutrient reactivity and societal management actions throughout large river basins.

Environmental Research Letters

Selected effects of the Conservation Reserve Program on program participants: A report to survey respondents

The Conservation Reserve Program (CRP) established under the 1985 Food Security Act was initially designed to provide the agricultural community economic assistance while protecting highly erodible cropland. Many of the environmental benefits to soil, water, and wildlife resources have been documented (Dunn and others, 1993; Ryan and others, 1998; Flather and others, 1999; Heard and others, 2000). However, the personal and social effects of the program on CRP participants (or contractees) had not been formally documented. Information had been limited to anecdotal comments from individual participants, such as: “since establishment of the CRP the streams have surface water in them” or “the CRP grasses capture drifting snow, making winter feeding of cattle easier.” The Farm Service Agency (FSA) and Natural Resources Conservation Service (NRCS) wanted to have a better picture of the strengths and weaknesses of the program, according to those most affected by it. In addition, policy makers wanted to get input from program participants on the growing emphasis of the program on long-term management and wildlife habitat requirements. As a result, the U.S. Geological Survey (USGS) was asked to survey CRP contractees on these issues. Preliminary results from this study have been furnished to the U.S. Department of Agriculture (USDA) and are being considered as new conservation and management policies for the CRP are being developed (as part of the recently passed Farm Security and Rural Investment Act of 2002). This report includes preliminary results of the study and is being sent to survey respondents. A formal publication of survey results is also being prepared and should be completed by the winter of 2002.

Open-File Report

A U.S.-China EcoPartnership study of disturbed wetland vegetation in West Dongting Lake, China

West Dongting Lake in China is important for human livelihoods and habitat of migratory waterfowl and other wildlife. The waterway re-engineering and agriculture intensification have contributed to changes in hydrology, sediment, and vegetation on the floodplain. This paper describes an EcoPartnership program conducted by the U.S. Geological Survey, Wetland and Aquatic Research Center, and Beijing Forestry University. It focused on the development of a wetland ecosystem network in West Dongting Lake with technical support from the U.S. partner using a number of related studies to examine wetland vegetation dynamics from upstream to downstream along the tributaries. The results of U.S. studies showed that the regeneration potential of species might be altered by changes in climate and local environment, and seed bank depletion by germination may be a major conservation threat in a future with recurring droughts in swamps of the southeastern United States. In the monsoonal wetlands of West Dongting Lake, the soil seed bank could be used as a seed source for revegetation after hydrologic restoration with the introduction of certain foundational species and the removal of poplar plantations. Also, West Dongting Lake is at high ecological risk of mercury pollution. Wetland ecosystem monitoring may allow managers to use the information to predict effects of climate change, water level and flow changes on sedimentation, and to manage for desired vegetation to support waterfowl and ecosystem services. The cooperation of two countries through the EcoPartnership program is now well established and poised for extensive research projects in the future.

West Dongting Lake

Development of a SWAT+ model to improve conservation practice efficacy for protecting stream health in karst pasturelands

Placing appropriate conservation practices in critical source areas of pollutants can benefit stream health in karst environments susceptible to agricultural pollution. Watershed models, such as the Soil and Water Assessment Tool+ (SWAT+), can optimize both practice selection and placement across the landscape, but to our knowledge no studies have tested this model in karst pasturelands. Our goal was to understand pollutant dynamics in pasturelands with karst topography in southwest Virginia, United States. We built a SWAT+ model to predict streamflow and pollutant loads for four watersheds. SWAT+ predicted that streamflow estimates were most affected by setting the available water capacity to zero and increasing the hydraulic conductivity of the soil, revealing that water, and associated pollutants, move primarily through subsurface pathways. Predicted sediment yield was negatively associated with agricultural land cover and was strongly influenced by channel erodibility—indicating that the predominant sediment source may be streambanks. Therefore, practices that stabilize stream banks (e.g., fencing cattle out of streams) may be most effective at reducing sediment loads. The model unsatisfactorily predicted total nitrogen and total phosphorus loads. The utility of SWAT+ for cattle grazing operations in karst regions could be improved by more accurately representing the effects of cattle grazing on streambank erosion and the dynamic subsurface movement of pollutants.

Virginia

Virus fate and transport during recharge using recycled water at a research field site in the Montebello Forebay, Los Angeles County, California, 1997-2000

Total and fecal coliform bacteria distributions in subsurface water samples collected at a research field site in Los Angeles County were found to increase from nondetectable levels immediately before artificial recharge using tertiary-treated municipal wastewater (recycled water). This rapid increase indicates that bacteria can move through the soil with the percolating recycled water over intervals of a few days and vertical and horizontal distances of about 3 meters. This conclusion formed the basis for three field-scale experiments using bacterial viruses (bacteriophage) MS2 and PRD1 as surrogates for human enteric viruses and bromide as a conservative tracer to determine the fate and transport of viruses in recycled water during subsurface transport under actual recharge conditions. The research field site consists of a test basin constructed adjacent to a large recharge facility (spreading grounds) located in the Montebello Forebay of Los Angeles County, California. The soil beneath the test basin is predominantly medium to coarse, moderately sorted, grayish-brown sand. The three tracer experiments were conducted during August 1997, August-September 1998, and August 2000. For each experiment, prepared solutions of bacteriophage and bromide were sprayed on the surface of the water in the test basin and injected, using peristaltic pumps, directly into the feed pipe delivering the recycled water to the test basin. Extensive data were obtained for water samples collected from the test basin itself and from depths of 0.3, 0.6, 1.0, 1.5, 3.0, and 7.6 meters below the bottom of the test basin. The rate of bacteriophage inactivation in the recycled water, independent of any processes occurring in the subsurface, was determined from measurements on water samples from the test basin. Regression analysis of the ratios of bacteriophage to bromide was used to determine the attenuation rates for MS2 and PRD1, defined as the logarithmic reduction in the ratio during each experiment. Although the inactivation rates increased during the third tracer experiment, they were nearly two orders of magnitude less than the attenuation rates. Therefore, adsorption, not inactivation, is the predominant removal mechanism for viruses during artificial recharge. Using the colloid-filtration model, the collision efficiency was determined for both bacteriophage during the second and third field-scale tracer experiments. The collision efficiency confirms that more favorable attachment conditions existed for PRD1, especially during the third tracer experiment. The different collision efficiencies between the second and third tracer experiments possibly were due to changing hydraulic conditions at the research field site during each experiment. The field data suggest that an optimal management scenario might exist to maximize the amount of recycled water that can be applied to the spreading grounds while still maintaining favorable attachment conditions for virus removal and thereby ensuring protection of the ground-water supply.

Scientific Investigations Report