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At least 397 records · Page 22Linked to original sources

The epizootiology of type C botulism in fish-eating birds at Salton Sea, California

During 1996, type C avian botulism killed over 15,000 fish-eating birds at the Salton Sea in southern California. Amont those affected were nearly 10,000 western white pelicans (Pelecanus erythrorhynchos) and over 1,200 endangered California brown pelicans (Pelecanus occidentalis californicus). Since 1996, smaller epizootics have occurred every year. Type C botulism is not typically associated with fish-eating birds. In the case of the Salton Sea, Mozambique tilapia (Oreochromis mossambicus) are the suspected source of type C toxin, although the mechanism by which the fish acquire the toxin is still unknown. The goals of this study were to: 1) Determine presence/absence of active Clostridium botulinum type C and type C botulinum toxin in tilapia in the Salton Sea. 2) Use geospatial analyses to evaluate relationships between patterns of mortality in birds and fish and presence/absence of toxin and/or toxin-producing bacteria in sediments and fish. We investigated a method of detecting C. botulinum type C cells in the intestinal contents of Mozambique tilapia. This method involved extraction of predominantly cellular DNA and uses a polymerase chain reaction assay to detect presence of type C toxin gene. We collected sick, dead and healthy fish from various sites throughout the Sea during the summers of 1999 to 2001 in order to test them for the presence of active C. botulinum type C by PCR and for the presence of type C toxin by ELISA and mouse test. The results demonstrate that the tilapia population in the Salton Sea harbors C. botulinum type C cells within their gastrointestinal tract and the prevalence of this organism varies from year to year. The total number of fish with toxin-producing bacteria was significantly greater in 2000 than in 2001. No difference in the numbers of positives was detected between sick and dead fish compared to live fish, and there were no differences noted with regard to location of fish collection. The prevalence of active type C toxin in tilapia was low and the majority of samples positive for toxin by ELISA were negative on mouse test. Further investigation is currently underway. From 1999-2001, routine surveillance was conducted July through November to detect morbidity and mortality in pelicans. Location of retrieval and total number of birds collected were documented. In all three years, the majority of botulism-affected pelicans were retrieved from the vicinities of the river deltas although the Whitewater River had the fewest birds of the three. It has yet to be determined what roles the tilapia and pelican populations play in influencing these patterns.

California

Founders of fish culture - European origins

Just where true fish culture appeared in history depends entirely upon what one considers fish culture to be. If the transportation of fishes from regions of plenty to those of few is to be regarded as fish culture - as it is by some even today - then this story should start in remotest antiquity and deal with an amazing series of failures. However, fish culture to be classed as a science must include far more than mere transportation, it must include a deliberate effort on the part of man to master a technique of fish raising which will yield results far superior to Nature's. Accordingly, the wheel of history must be spun forward to the fifteenth century, A. D., when man first conceived the idea that with care and exactitude, he could improve upon Nature. The fish cultural efforts of the Chinese, the Egyptians, the Greeks, and the Romans may be skipped over in a hurry, for they represented little more than the transportation and rearing of wild fish. With the renaissance of modern civilization in Europe came the birth of scientific fish culture.

Progressive Fish-Culturist

Predictability of littoral-zone fish communities through ontogeny in Lake Texoma, Oklahoma-Texas, USA

We sampled larval, juvenile and adult fishes from littoral-zone areas of a large reservoir (Lake Texoma, Oklahoma-Texas) (1) to characterize environmental factors that influenced fish community structure, (2) to examine how consistent fish-environment relationships were through ontogeny (i.e., larval vs. juvenile and adult), and (3) to measure the concordance of larval communities sampled during spring to juvenile and adult communities sampled at the same sites later in the year. Larval, juvenile and adult fish communities were dominated by Atherinidae (mainly inland silverside, Menidia beryllina) and Moronidae (mainly juvenile striped bass, Morone saxatilis) and were consistently structured along a gradient of site exposure to prevailing winds and waves. Larval, juvenile and adult communities along this gradient varied from atherinids and moronids at highly exposed sites to mostly centrarchids (primarily Lepomis and Micropterus spp.) at protected sites. Secondarily, zooplankton densities, water clarity, and land-use characteristics were related to fish community structure. Rank correlation analyses and Mantel tests indicated that the spatial consistency and predictability of fish communities was high as larval fishes sampled during spring were concordant with juvenile and adult fishes sampled at the same sites during summer and fall in terms of abundance, richness, and community structure. We propose that the high predictability and spatial consistency of littoral-zone fishes in Lake Texoma was a function of relatively simple communities (dominated by 1-2 species) that were structured by factors, such as site exposure to winds and waves, that varied little through time. ?? Springer 2005.

Environmental Biology of Fishes

The role of the diagnostic laboratory in fish disease control

The diagnostic laboratory is becoming increasingly important to the productivity and profitability of hatcheries, fish farms, and aquaculture stations. The basic function of the laboratory personnel is to isolate and identify viral, bacterial, protozoan, and other fish pathogens present in cultured and feral fish stocks. New, rapid and accurate methods for the detection and identification of fish disease agents based on immunological, biochemical, and physiological assays are becoming commonly used. Nearly every North American state or province and many foreign countries have fish health regulations that require inspection of stocks for certain disease agents before the fish are shipped into their areas. Decisions from the diagnostic laboratory on identification, treatment, guaranteed isolation, immunization, and disposal of fish populations affect administrative directives, hatchery placement, and national and international transportation of fish and fish products. This paper reviews concepts and describes the equipment, supplies, biologics, and media needed for the basic diagnostic laboratory. Information management, including training of staff, certification procedures, and quality control are also discussed.

Annual Review of Fish Diseases

The concept of stress in fish

The general physiological response of fish to threatening situations, as with all vertebrates, is referred to as stress . A stress response is initiated almost immediately following the perception of a stressor. Mildly stressful situations can have beneficial or positive effects (eustress), while higher severities induce adaptive responses but also can have maladaptive or negative consequences (distress). The stress response is initiated and controlled by two hormonal systems , those leading to the production of corticosteroids (mainly cortisol) and catecholamines (such as adrenaline and noradrenaline and their precursor dopamine). Together these regulate the secondary stress response factors that alter the distribution of necessary resources such as energy sources and oxygen to vital areas of the body, as well as compromise hydromineral imbalance and the immune system. If fish can resist death due to a stressor, they recover to a similar or somewhat similar homeostatic norm. Long-term consequences of repeated or prolonged exposures to stress are maladaptive by negatively affecting other necessary life functions (growth, development, disease resistance, behavior, and reproduction), in large part because of the energetic cost associated with mounting the stress response (allostatic load). There is considerable variation in how fish respond to a stressor because of genetic differences among different taxa and also within stocks and species. Variations within the stress response are introduced by the environmental history of the fish, present ambient environmental conditions, and the fish's present physiological condition. Currently, fish physiology has progressed to the point where we can easily recognize when fish are stressed, but we cannot always recognize when fish are unstressed because the lack of clinical signs of stress does not always correspond to fish being unstressed. In other words, we need to be aware of the possibility of false negatives regarding clinical signs of stress. In addition, we cannot use clinical data to precisely or accurately infer severity of a stressor.

Fish Physiology

Potential population and assemblage influences of non-native trout on native nongame fish in Nebraska headwater streams

Non-native trout are currently stocked to support recreational fisheries in headwater streams throughout Nebraska. The influence of non-native trout introductions on native fish populations and their role in structuring fish assemblages in these systems is unknown. The objectives of this study were to determine (i) if the size structure or relative abundance of native fish differs in the presence and absence of non-native trout, (ii) if native fish-assemblage structure differs in the presence and absence of non-native trout and (iii) if native fish-assemblage structure differs across a gradient in abundances of non-native trout. Longnose dace Rhinichthys cataractae were larger in the presence of brown trout Salmo trutta and smaller in the presence of rainbow trout Oncorhynchus mykiss compared to sites without trout. There was also a greater proportion of larger white suckers Catostomus commersonii in the presence of brown trout. Creek chub Semotilus atromaculatus and fathead minnow Pimephales promelas size structures were similar in the presence and absence of trout. Relative abundances of longnose dace, white sucker, creek chub and fathead minnow were similar in the presence and absence of trout, but there was greater distinction in native fish-assemblage structure between sites with trout compared to sites without trout as trout abundances increased. These results suggest increased risk to native fish assemblages in sites with high abundances of trout. However, more research is needed to determine the role of non-native trout in structuring native fish assemblages in streams, and the mechanisms through which introduced trout may influence native fish populations.

Nebraska

Do intracoelomic telemetry transmitters alter the post-release behaviour of migratory fish?

Electronic tags have become a common tool in fish research, enhancing our understanding of how fish interact with their environment and move among different habitats, for estimating mortality and recording internal physiological states. An often-untested assumption of electronic tagging studies is that tagged fish are representative of untagged conspecifics and thus show ‘normal’ behaviour (e.g. movement rates, swimming activity, feeding). Here, we use a unique data set for potamadromous walleye (Sander vitreus) in Lake Huron and Lake Erie tributaries to assess whether the lack of appropriate controls in electronic tagging could seriously affect behavioural data. We used fish tagged in previous years and compared their migratory behaviour during the spawning season to fish tagged in a current year at the same location. The objective of the study was to determine whether intracoelomic acoustic tag implantation altered downstream movement of walleye after spawning. Fish tagged in a given season travelled slower downstream from two river spawning sites than fish tagged in previous years. Fish tagged one or two years earlier showed no differences between each other in downstream travel time, in contrast to fish tagged in a given year. Our results support notions that standard collection and intracoelomic tagging procedures can alter short-term behaviour (i.e. days, weeks, months), and as such, researchers should use caution when interpreting data collected over such time periods. Further, whenever possible, researchers should also explicitly evaluate post-tagging effects on behaviour as part of their experimental objectives.

Maumee Bay, Saginaw Bay

Biogeographic freshwater fish pattern legacy revealed despite rapid socio-economic changes in China

Understanding drivers of freshwater fish assemblages is critically important for biodiversity conservation strategies, especially in rapidly developing countries, which often have environmental protections lagging behind economic development. The influences of natural and human factors in structuring fish assemblages and their relative contributions are likely to change given the increasing magnitude of human activities. To discriminate natural and human drivers of fish diversity and assemblage patterns in developing countries with rapid socio-economic development, a dataset of 908 freshwater fish species and 13 metrics including three categories of both natural (i.e., biogeographic) and human drivers (i.e., economic growth, inland fisheries) in China were analysed with machine learning algorithms (i.e., self-organizing map, random forest). Here, we found that biogeographic drivers explained 21.8% of the observed fish assemblage patterns in China and remained stronger predictors when compared to human drivers (i.e., 15.6%, respectively). Freshwater fish species richness was positively correlated to rainfall, air temperature, surface water area and inland fisheries production but negatively correlated with urbanization. In addition, the strong structuring effects of climatic variables on Chinese fish richness patterns suggested that the fish assemblages could be particularly vulnerable to climate change. Our results showed that natural biogeographic factors still dominate in driving freshwater fish assemblage patterns despite increased human disturbances on aquatic ecosystems in a rapidly developing country. These findings consequently suggested that we should consider both natural (e.g., climate) and human (e.g., urbanization, inland fisheries) factors when establishing aquatic conservation strategies and priorities for developing countries that are experiencing rapid socio-economic changes.

Fish and Fisheries

Effects of capture by trammel net on Colorado River native fishes

Trammel nets are commonly used to sample rare fishes; however, little research has assessed delayed mortality associated with this capture technique. We conducted laboratory experiments to evaluate the effects of capture by trammel net on bonytail Gila elegans, razorback sucker Xyrauchen texanus, and roundtail chub Gila robusta, at 15, 20, and 25uC. Fish (139–288 mm total length) were entangled in a trammel net for 2 h or captured by seine net and then monitored for mortality for at least 14 d. Blood samples were collected immediately after capture, and plasma cortisol levels were quantified as an index of capture-related stress. The cortisol response varied by species, but mean cortisol levels were higher for fish captured by trammel netting (295.9 ng/mL) relative to fish captured by seine netting (215.8 ng/mL). Only one fish (of 550) died during capture and handling, but 42% of the trammel-netted fish and 11% of the seine-netted fish died within 14 d after capture. In general, mortality after capture by trammel net increased with increased water temperature and at 25uC was 88% for bonytail, 94% for razorback sucker, and 25% for roundtail chub. Delayed mortality of wild-caught fish captured by trammel net has the potential to be high, at least under some circumstances. We suggest that sampling frequency, timing of sampling (relative to reproductive cycles), and water temperature all be considered carefully when using trammel nets to sample diminished populations of imperiled native fishes.

Colorado River

Slow recovery of headwater-stream fishes following a catastrophic poisoning event

Accidental spills of chemicals and other pollutants can decimate populations of stream-dwelling species. Recovery from such accidents can be relatively fast and complete when the affected stream reaches can be recolonized from upstream and downstream sources. However, faunal recoveries from accidental spills that extirpate populations from entire headwater streams have not been extensively documented, and understanding resilience of headwater-stream biota is relevant for assessing threats to at-risk species. We assessed recovery of fish populations in a 5.7-km long headwater stream in the southeastern United States following a complete, or nearly complete, fish-kill caused by a chemical spill near the source of the stream. We sampled for fishes at five stream locations, two downstream and three upstream from a perched, culverted road-crossing located 2.4 km upstream from the stream mouth, over a period of 18.5 months following the poisoning event. We observed 11 fish species, representing <65% of the fish species expected based on occurrences in nearby tributary streams. In post-poisoning sampling, only three of these taxa were observed upstream of the culvert; all 11 species, including the federally threatened Cherokee Darter Etheostoma scotti, were found downstream of the culvert but were mostly represented by a few, large individuals. In contrast, dead individuals of at least eight taxa including the Cherokee Darter were observed upstream of the culvert at the time of the fish-kill. These observations provide evidence of slow recovery of a headwater fish fauna, and especially upstream of a barrier to fish movement, where the recolonization sources are primarily downstream. Additional case studies may reveal whether this result applies generally to headwater streams. Slow recovery could make species that primarily inhabit or maintain greatest abundances in headwaters, including multiple at-risk fishes, particularly vulnerable to the threat of accidental spills that result in local population extirpation.

Journal of Fish and Wildlife Management

Disease control in hatchery fish

A direct method for controlling diseases among hatchery fish has long been sought as an alternative to the earlier technique of hand dipping. A simple, practical, effective method of direct pond treatment not only obviates all need for handling fish weakened by disease, but it would materially reduce the time and effort required by hatcherymen in keeping diseases under control. Furthermore, a direct pond treatment could be used for routine prophylaxis to eradicate diseases during their incipient stages--long before their presence becomes apparent. Several methods for applying direct pond treatments in coldwater-fish culture have been proposed. Kingsbury and Embody (1932) developed a technique for applying disinfectants directly to the inflowing water of a pond. Later, Fish (1939) advocated a method involving the recirculation of a disinfecting solution through a fish pond. For various reasons, neither of these methods proved to be an effective answer to the problem of controlling hatchery diseases and neither became widely adopted. The method described herein has been extensively tested, both in the laboratory and at the producing hatchery, over a period of several years. Once familiarity with the details of application have been mastered, th8 reduction in effort required to treat fish is amazing. For example, two men have treated 20 large ponds containing several million fish, in one morning with no significant increase in mortality of the fish, whereas a crew of eight men required a full day to treat a single similar pond by hand dipping the fish with a subsequent loss approximating 50 percent of the stock.

Fishery Leaflet

A report upon the Grand Coulee Fish Maintenance Project 1939-1947

The construction or Grand Coulee Dam, on the upper Columbia River, involved the loss of 1,140 lineal miles of spawning and rearing stream to the production of anadromous fishes. The fact that the annual value of these fish runs to the nation was estimated at $250,000 justified reasonable expenditures to assure their perpetuation. It was found economically infeasible to safely collect and pass adult fish upstream and fingerling fish downstream at the dam because of the tremendous flow of the river and the 320 foot vertical difference in elevation between forebay and tailrace. The Grand Coulee Fish-Maintenance Project, undertaken by the United States Fish and Wildlife Service in 1939, consisted in relocating the anadroumous runs of the upper Columbia River to four major tributaries entering below the Grand Coulee damsite. These streams were believed capable of supporting several times their existing, badly depleted, run. The plan was predicated upon the assumption that the relocated runs, in conformity with their "homing tendency", would return to the lower tributaries rather than attempt to reach their ancestral spawning grounds above Grand Coulee Dam. This interim report covers the history and accomplishments of the Grand Coulee Fish-Maintenance Project through the initial period of relocating the rune as well as the first four years of the permanent program. Results obtained to date indicate conclusive success in diverting the upper Columbia fish runs into the accessible lower tributaries. The results also indicate, less conclusively, that - in spite of many existing handicaps - the upper Columbia salmon and steelhead runs may be rehabilitated through the integrated program of natural and artificial propagation incorporated in the Grand Coulee Fish-Maintenance Project.

Washington

3D habitat complexity and coral morphology modulate reef fish functional structure in a marine national park

The ongoing degradation of coral reef habitats is widely acknowledged to have adverse effects on the abundance and diversity of reef fish populations, yet the direct effects on ecosystem functions remain uncertain. This study used a quantitative approach to determine the mechanistic links between fish assemblages and ecological function. We investigated the effects of 3D habitat structure and coral morphology on the ecological, behavioral, and morphological functional traits of reef fish within a protected marine national park. Fish traits such as Gregariousness, Water Column Position, and Body Shape were identified to be highly influential in shaping the multidimensional fish functional space, which was categorized into 10 Fish Functional Groups (FFG). Furthermore, habitat complexity and coral morphology significantly explained the abundances of eight out of 10 FFG. Notably, the habitat complexity metrics of Slope and Surface Complexity, along with coral morphologies of Branching and Mounding types, emerged as the most influential habitat features across FFG. Pairing Compressiform species and Schooling Short/Deep species, for example, significantly increased in abundance on substrate with higher Slopes and increased percentages of branching coral cover. Additionally, Cryptic and Nocturnal species exhibited statistically significant associations with all coral morphologies and substrates with high trait values of Slope and Curvature. Elucidating ecological drivers of specific functional groups of reef fish is critical for determining how changes in reef composition and structure will alter fish assemblages. Broad scale patterns were also detected, suggesting that although structural complexity is important, live coral morphologies have a greater positive impact on reef fish functional groups. These findings have direct implications for conservation and monitoring efforts, offering valuable insights for predicting the impacts of environmental change on community dynamics and ecosystem functioning.

Hawaii

Wetland management strategy to reduce mercury export in water and bioaccumulation in fish

Wetland environments provide numerous ecosystem services but also facilitate methylmercury (MeHg) production and bioaccumulation. We developed a wetland‐management technique to reduce MeHg concentrations in wetland fish and water. We physically modified seasonal wetlands by constructing open‐ and deep‐water treatment cells at the downstream end of seasonal wetlands to promote naturally occurring MeHg‐removal processes. We assessed the effectiveness of reducing mercury (Hg) concentrations in surface water and western mosquitofish that were caged at specific locations within 4 control and 4 treatment wetlands. Methylmercury concentrations in wetland water were successfully decreased within treatment cells during only the third year of study; however, treatment cells were not effective for reducing total Hg concentrations. Furthermore, treatment cells were not effective for reducing total Hg concentrations in wetland fish. Mercury concentrations in fish were not correlated with total Hg concentrations in filtered, particulate, or whole water; and the slope of the correlation with water MeHg concentrations differed between months. Fish total Hg concentrations were weakly correlated with water MeHg concentrations in April when fish were introduced into cages but were not correlated in May when fish were retrieved from cages. Fish total Hg concentrations were greater in treatment wetlands than in control wetlands the year after the treatment wetlands’ construction but declined by the second year. During the third year, fish total Hg concentrations increased in both control and treatment wetlands after an unexpected regional flooding event. Overall, we found limited support for the use of open‐ and deep‐water treatment cells at the downstream end of wetlands to reduce MeHg concentrations in water but not fish. We suggest that additional evaluation over a longer period of time is necessary.

Environmental Toxicology and Chemistry

From forests to fish: Mercury in mountain lake food webs influenced by factors at multiple scales

Mountain lakes, while seemingly pristine, have been subjected to historical fish stocking practices and exposure to atmospherically deposited contaminants like mercury. Mercury bioaccumulation in these ecosystems varies widely due to strong environmental gradients, and there are complex, hierarchical factors that affect mercury transport and loading, methylmercury production, and food web biomagnification. We sought to assess how representative variables associated with watershed, lake, and food web‐scale processes—specifically, catchment tree cover, lake benthic primary production, and fish diet, respectively—are associated with mercury concentrations in mountain lake fish. Mean fish mercury concentrations varied threefold between lakes, with nearshore tree cover and fish diet accounting for the most variance in fish mercury. Tree cover was likely positively correlated to fish Hg due to its contributions to local deposition and its effect on lake biogeochemistry. Fish with benthic diets tended to have higher mercury concentrations, illustrating that food web processes are an important consideration when investigating drivers of contaminant bioaccumulation. Our results suggest that both landscape and ecological factors are determinants of fish mercury bioaccumulation, and thus variables at multiple scales should be considered when managing mountain lake food webs for mercury exposure risk.

Washington

Is chemical control for crayfish in hatchery fish shipments practical?

Invasive crayfish (family Cambaridae) displace native crayfish species and alter aquatic habitat, community structure, and ecosystem function. We evaluated whether chemical control can be a reliable control agent for crayfish to ensure that shipments from fish hatcheries did not result in new infestations of invasive crayfish. A series of acute (≤1 h) toxicity tests were conducted to evaluate the toxicity of cypermethrin and pyrethrin to crayfish, freshwater mussels, and fish; chemical concentrations in test organisms; effectiveness of carbon-block filters to remove cypermethrin from test waters; and the cost of chemical control relative to extra handling of fish. Cypermethrin dosed at 75 μg/L for 15 min resulted in 100% mortality of adult white river crawfish Procambarus acutus and virile crayfish Faxonius virilis but did not cause >20% mortality in adult pondmussel Ligumia subrostrata , juvenile fatmucket Lampsilis siliquoidea , fingerling Bluegill Lepomis macrochirus , hybrid sunfish (Bluegill × Green Sunfish L. cyanellus ), hybrid Striped Bass (White Bass Morone chrysops × Striped Bass M. saxatilis ), yearling Paddlefish Polyodon spathula, or ready-to-eat Bluegill, hybrid sunfish, and Channel Catfish Ictalurus punctatus . Behavioral effects, such as loss of equilibrium or head shaking, were generally limited to 1 h postexposure. Mean concentrations of cypermethrin increased in fish fillets (4–26 μg/g) and whole fish (5–1,770 μg/g); therefore, regulations limiting harvest for up to 7 d following stocking may be required. A carbon-block filtration system was effective in reducing (<90%) cypermethrin concentrations and thus reducing potential effects to nontarget species in receiving waters. Extra handling of fish was more cost-effective for all fish tested except for Paddlefish, where the cost of chemical control was half that for extra handling. For all other fish tested, chemical control was 4–10 times more expensive than extra handling. Special use permits or chemical registration are needed before chemical control for crayfish could be routinely used at fish hatcheries.

North American Journal of Aquaculture

Fish diversity, endemism, threats, and conservation in the Jinsha River basin (upper Yangtze River), China

The Jinsha River, which comprises the upper reaches of the Yangtze River, has among the highest freshwater fish biodiversity and endemism in China, but these characteristics have rarely been quantitatively evaluated at the basin scale. We used fish presence–absence data collected from the entire Jinsha River basin (JRB) from 1964 to 2017 to determine patterns in fish biodiversity. In total, 229 freshwater fish species from 9 orders, 26 families, and 110 genera were recorded. Of these species, 161 were endemic to China, with 94 species being endemic to the Yangtze River basin, and 39 species were threatened. Fish species richness was higher in the downstream river reaches and was higher in the main stem than in the tributaries. Overfishing, water pollution, and dam construction have been threatening fish diversity in the JRB for several decades. Conservation strategies similar to those used in North America may be applicable to the JRB to help protect native fishes in this important river basin. Such strategies include (1) assessment of several tributaries as fish reserves; (2) regular adjustment of turbine operations during the fish spawning period; and (3) regulation of the many co-occurring human stressors in the JRB.

Jinsha River Basin (Upper Yangtze River)

Retention of p-Chip microtransponders and posttagging survival of small-bodied stream fishes

Objective Obtaining demographic rates often requires complex open-population capture–mark–recapture (CMR) study designs. Conducting such studies for small-bodied fishes has been limited in part by excessive mortality after tagging procedures and poor tag retention. As new tag types emerge, information regarding fish survival and tag retention over varying time scales may benefit resource managers to effectively plan future CMR studies. The p-Chip microtransponder is a 500- × 500- × 100-μm tag that is inserted subcutaneously and is read with a handheld laser. Each tag contains a nine-digit unique identification number. P-Chip microtransponder tags have been used on a limited number of small-bodied fishes, with relatively high rates observed for fish survival and tag retention. Information on posttagging survival and retention of p-Chip microtransponder tags across a range of small-bodied fish species and tagging locations is needed to inform their effectiveness in future CMR studies. Methods We quantified survival and tag retention after p-Chip microtransponder implantation in Creek Chub Semotilus atromaculatus and Northern Pearl Dace Margariscus nachtriebi in a laboratory environment, and retention of p-Chip microtransponder tags in Northern Pearl Dace was also quantified over a year-long field study. We marked 56 Creek Chub and 9 Northern Pearl Dace in the laboratory with p-Chip microtransponder tags and compared them to an equal number of unmarked individuals over 90 days. We marked 1990 Northern Pearl Dace and conducted recapture events through four seasons (June, September, and October 2022 and June 2023) in four headwater streams of Nebraska. Result Survival for Creek Chub was 85% (standard error [SE] = 5.9) and did not differ from control fish survival (95%; SE = 3.2) in the 90-day laboratory experiment. Survival for Northern Pearl Dace was 89% (SE = 11.0) and did not differ from that of control fish (100%) in the laboratory experiment. Tag retention was 89% (SE = 4.6) for Creek Chub and 100% for Northern Pearl Dace in the laboratory. The p-Chip microtransponder performed well during the CMR field study, with tag retention for Northern Pearl Dace at 94% across 374 days. Conclusion Our results suggest that the p-Chip microtransponder minimally affected small-bodied fish survival and had high tag retention in both the laboratory and field studies. Thus, the p-Chip microtransponder tag may be appropriate for use in small-bodied fishes when individual identification is needed in a CMR study.

North American Journal of Fisheries Management