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Effects of a low-lipid diet on the gut microbiome and head kidney transcriptome of juvenile Chinook Salmon

Objective Pacific salmon Oncorhynchus spp. reared in production hatcheries are typically fed high-lipid, energy-dense diets to achieve large size and high body condition prior to release. In contrast, juveniles in natural environments tend to consume low-lipid, high-protein diets, and fish reared for research or conservation purposes are sometimes fed diets that are formulated to mimic natural diets and promote wild-like phenotypes. Understanding how these alternative diets affect fish health beyond growth and body condition could ultimately contribute to improving hatchery fish fitness. Methods In this work, we evaluated changes in the fecal microbiome and gene expression of juvenile Chinook Salmon O. tshawytscha on a standard high-lipid hatchery diet versus a low-lipid diet formulated to mimic the nutrition profile of natural-origin fish. To evaluate the time scale at which diet alters the fecal microbiome, we collected longitudinal samples over a 12-week period and switched the diets of a subset of fish twice during the experiment. We used 16S ribosomal RNA gene amplicon sequencing to characterize fecal microbiome differences between fish on the two diets as well as hatchery-reared fish at a production hatchery, hatchery fish that had been captured after release into a stream, and natural-origin, stream-reared fish of similar ages. Additionally, we conducted RNA sequencing on head kidney samples from laboratory-reared fish to evaluate changes in gene expression in this important immune organ. Results We found that the low-lipid diet and the hatchery diet resulted in microbiomes that differed from the microbiome of natural-origin fish and from each other and that diet-driven changes to the microbiome could occur in under 14 d. The low-lipid diet did not result in a microbiome that resembled the microbiome of naturally produced fish. Instead, the low-lipid diet resulted in a microbiome community that was distinct from those of fish reared on the hatchery diet and fish sampled from the wild. The RNA sequencing results indicated differential enrichment of pathways related to immunity, metabolism, and hormone synthesis between fish that were fed the two experimental diets. Conclusions The results suggest that additional environmental factors influence the microbiome more strongly than diet formulation or that the low-lipid diet has a smaller effect on the microbiome than a natural, ­invertebrate-based diet. Given that the gut microbiome and systemic immune function contribute significantly to disease resistance, our findings highlight the importance of understanding how diets fed to fish in captivity may affect fish health beyond growth and body condition metrics.

Journal of Aquatic Animal Health

Multiple-well monitoring site adjacent to the Midway- Sunset and Buena Vista Oil Fields, Kern County, California

Groundwater quality in and around oil fields in the Southern San Joaquin Valley is of interest to many California residents that rely heavily on groundwater for domestic, commercial, and agricultural use. To help assess the effects of historical oil-field activities and natural geologic sources on groundwater near the southwest margins of the Kern County Groundwater Subbasin, a multiple-well monitoring site was installed near the administrative boundary between the Midway-Sunset and Buena Vista Oil Fields in Kern County, California. The installation of the Midway-Sunset Buena Vista multiple-well monitoring site (MSBV) supports regional analysis of the relations of oil and gas sources to groundwater quality by providing information about the geology, hydrology, geophysical properties, and water quality of the alluvial and upper Tulare aquifers in areas where groundwater data were limited. Data collected from the site included drill cuttings, whole core samples, sidewall core samples, mud-gas analysis, borehole geophysical logs, depth to water measurements, and water quality samples. Whole cores were scanned using dual energy computed tomography. Subsamples of selected cores were analyzed for density, porosity, specific retention, and bulk minerology. Thin sections of the subsamples were prepared, photographed, and examined. Two samples were analyzed using scanning electron microscope technology to examine the microporosity of diatomite laden sediment. Instrumentation installed in the wells collect hourly depth to water measurements. Analysis of the data show there is 355 feet of alluvium overlying the Tulare Formation at the well site. The contact between the two formations is an aquitard resulting in a perched aquifer in the alluvium and unconfined aquifer in the Tulare Formation. The alluvium is more heterogenous and finer grained than the Tulare Formation resulting in markedly higher porosity in the alluvium compared to the Tulare Formation. Higher specific retention observed in the alluvium is attributed to the finer grained sediment and greater abundance of reworked diatomite (as represented by opal-CT [cristobalite-tridymite]) compared to the Tulare Formation. Total dissolved solids (TDS) approached or exceeded 10,000 milligrams per liter (mg/L) in the alluvium from approximately 176 to 242 feet below land surface and at the top of the Amnicola clay at approximately 670 feet below land surface within the Tulare Formation. Elevated TDS, chloride, and boron concentrations in the alluvium and on top of the Amnicola clay likely reflect groundwater that is mixed with oil-field water. Water chemistry and modern-aged groundwater in the alluvial monitoring well (MSBV #3) are consistent with the oil-field water in the alluvium being derived from documented historical surface disposal of oil-field water upslope (northwest) of the site. Water chemistry and pre-modern groundwater age in the deeper Tulare monitoring well (MSBV #1) on top of the Amnicola clay are consistent with oil-field fluids derived from upslope natural geologic sources or old oil wells that leak in the subsurface. Shallow groundwater in the Tulare (MSBV #2) is not affected by mixing with oil-field sources.

EarthArXiv

New methodology for assessing underground natural gas storage resources – Example from Michigan Basin, United States

Energy consumption in the United States (U.S.) and across the world is shifting away from traditional fossil fuels like coal and oil, and towards natural gas and renewable sources, including hydrogen. Because gas demand is typically greatest during cold seasons and renewable sources sometimes produce variable supplies, it is important to store energy for use when demand exceeds supply. Whereas batteries and tanks typically store energy above ground, geologic (underground) storage may be able to retain much greater quantities of energy over much longer durations (e.g., Matos et al. 2019; Buursink et al. 2023). Consequently, the U.S. Geological Survey (USGS) is developing a methodology to locate new geologic energy storage sites with an initial focus on depleted hydrocarbon reservoirs followed by estimates of associated pore space or gas storage capacity. Depleted hydrocarbon fields in Michigan Basin in the U.S. already host large quantities of injected natural gas (about 681 billion cubic feet of seasonal gas capacity in 42 gas storage facilities; U.S. Energy Information Administration 2024). Therefore, we used this basin as an example to assess potential new storage resources alongside existing storage capacity and carbon dioxide sequestration resources (e.g., Katz and Coats 1968; Haagsma et al. 2020). In Michigan Basin gas storage is taking place in reservoirs within several key hydrocarbon producing formations with different lithologies, including the informal Stray sandstone of the Michigan Formation and the pinnacle reefs of the Niagara Group (Gautier et al. 1995; Swezey et al. 2015). The assessment methodology introduced here consists of two steps relying on both well information and reservoir data.

Michigan

Current status of the community sensor model standard for the generation of planetary digital terrain models

The creation of accurate elevation models (topography) from stereo images are critical for a large variety of geospatial activities, including the production of digital orthomosaics, change detection, landing site analysis, geologic mapping, rover traverse planning, and spectral analysis. The United Stated Geological Survey, Astrogeology Science Center, continues to transition the supported planetary sensor models to the Community Sensor Model (CSM) standard. This paper describes the current state of use for this photogrammetric standard, supported sensor model types, and qualitatively compares derived topography between SOCET SET and SOCET GXP ( ® BAE Systems) using HiRISE stereo images of Mars. Our transition to the CSM standard will ensure an uninterrupted capability to make these valuable products for Mars and many other extraterrestrial planets and moons.

Remote Sensing

Evaluation of models for estimating hydraulic conductivity in glacial aquifers from NMR logging

Nuclear magnetic resonance (NMR) logging is a promising method for estimating hydraulic conductivity ( K ). During the past ∼60 years, NMR logging has been used for petroleum applications, and different models have been developed for deriving estimates of permeability. These models involve calibration parameters whose values were determined through decades of research on sandstones and carbonates. We assessed the use of five models to derive estimates of K in glacial aquifers from NMR logging data acquired in two wells at each of two field sites in central Wisconsin, USA. Measurements of K , obtained with a direct push permeameter (DPP), K DPP , were used to obtain the calibration parameters in the Schlumberger-Doll Research, Seevers, Timur-Coates, Kozeny-Godefroy, and sum-of-echoes (SOE) models so as to predict K from the NMR data; and were also used to assess the ability of the models to predict K DPP . We obtained four well-scale calibration parameter values for each model using the NMR and DPP measurements in each well; and one study-scale parameter value for each model by using all data. The SOE model achieved an agreement with K DPP that matched or exceeded that of the other models. The Timur-Coates estimates of K were found to be substantially different from K DPP . Although the well-scale parameter values for the Schlumberger-Doll, Seevers, and SOE models were found to vary by less than a factor of 2, more research is needed to confirm their general applicability so that site-specific calibration is not required to obtain accurate estimates of K from NMR logging data.

Wisconsin

Comparative assessment of a restored and natural wetland using 13C-DNA SIP reveals a higher potential for methane production in the restored wetland

Wetlands are the largest natural source of methane (CH 4 ), a potent greenhouse gas produced by methanogens. Methanogenesis rates are controlled by environmental factors such as redox potential, temperature, and carbon and electron acceptor availability and are presumably dependent on the composition of the active methanogen community. We collected intact soil cores from a restored and natural freshwater depressional wetland on Maryland’s Delmarva Peninsula (USA) to assess the effects of wetland restoration and redox shifts on microbial processes. Intact soil cores were incubated under either saturated (anoxic) or unsaturated (oxic) conditions and amended with 13 C-acetate for quantitative stable isotope probing (qSIP) of the 16S rRNA gene. Restored wetland cores supported a distinct community of methanogens compared to natural cores, and acetoclastic methanogens putatively identified in the genus Methanosarcina were among the most abundant taxa in restored anoxic and oxic cores. The active microbial communities in the restored wetland cores were also distinguished by the unique presence of facultatively anaerobic bacteria belonging to the orders Firmicutes and Bacteroidetes . In natural wetland incubations, methanogen populations were not among the most abundant taxa, and these communities were instead distinguished by the unique presence of aerobic bacteria in the phyla Acidobacteria , Actinobacteria , and class Alphaproteobacteria . Iron-reducing bacteria, in the genus Geobacter , were active across all redox conditions in both the restored and the natural cores, except the natural oxic–anoxic condition. These findings suggest an overall higher potential for methanogenesis in the restored wetland site compared to the natural wetland site, even when there is evidence of Fe reduction.

Maryland

Waning greenhouse gas emissions from U.S. Federal lease coal production by the mid-21st century

This study presents estimates of future years (2024–2051) United States Federal lease coal production and the resulting greenhouse gas (GHG) emissions from the combustion, transport, and mining of that fuel. Results from the coal production estimate indicate a decline in production from Federal leases; with known production of 240 million short tons (mtn) in 2023 and a projected decline to 34.0 mtn by 2051, which represents a reduction to 14.2% of the 2023 value. In parallel with this projection, total GHG emissions are estimated to decrease from 402.2 million metric tons of carbon dioxide equivalent (MMT CO 2 eq.) in 2024 to 55.0 MMT CO 2 eq. in 2051, a decline to 13.7% of 2024 emissions estimates. The reductions in coal production and emissions are mainly the result of planned coal combustion power plant closures, with major projected closures in 2037 and 2048. However, GHG emissions estimates for future years can be uncertain as they rely heavily on coal production estimates from operators' public business plans and other publicly available resources. Forward looking plans of this type are subject to significant changes if economic and political factors deviate from current information. Results suggest that average GHG emissions over the time series breakout to 95% end point combustion, 3.7% transportation combustion emissions, and 1.3% fugitive emissions, although there is uncertainty associated with these figures. Uncertainty stemming from production projections, sector distributions, and emissions factors on the future emissions estimates increases with time, ranging from −28% to +48% within the 2024–2051 timeframe.

Alabama, Colorado, Montana, North Dakota, Utah, Wy

Spatiotemporal variability of algal biomass and nitrate in Owasco and Seneca Lakes in the Finger Lakes Region, New York, in 2019

Cyanobacterial harmful algal blooms (CyanoHABs) have become increasingly common, threatening the security of water resources globally. The U.S. Geological Survey conducted high-resolution nearshore mapping surveys using boat-mounted multiparameter sondes and nitrate sensors during the summer and fall of 2019 on Owasco Lake and Seneca Lake, two lakes with documented CyanoHABs in the Finger Lakes region of New York State. Discrete sensor measurements and water-quality samples were collected at fixed points along survey routes and continuous data were generated at open-water monitoring platforms. This investigation examined whether water-quality information from nearshore surveys was representative of open-water conditions and if nearshore surveys could be used to identify areas with localized nearshore CyanoHABs and potential sources of nutrients not captured by tributary sampling. In addition to comparisons across methods, nearshore concentrations of nitrate and chlorophyll were evaluated relative to tributary outlets, cyanobacterial abundance and biovolume at discrete locations, and the locations of near-surface CyanoHABs that were designated as “confirmed with high toxins” by the New York State Department of Environmental Conservation. Nitrate and chlorophyll concentrations were comparable across methods for each lake, although concentration ranges were typically higher for nearshore mapping datasets than for nearshore discrete datasets. Nearshore surveys indicated areas of nitrate enrichment that varied temporally in both lakes. Orthophosphate was not routinely detected. Across methods, median chlorophyll concentrations were higher for the summer survey than for the fall survey in Owasco Lake. Nearshore chlorophyll concentrations varied more temporally in Owasco Lake than in Seneca Lake. Phytoplankton and cyanobacterial abundance and biovolume were about five times higher in Owasco Lake than in Seneca Lake. Cyanobacteria dominated the phytoplankton community in most samples, and Microcystis comprised the bulk of the cyanobacterial biovolume. The most abundant potential cyanotoxin-producing (specifically microcystins) genera were Microcystis , Synechococcus , Aphanocapsa , and Pseudanabaena . The cyanobacterial community composition was comparable between open-water monitoring platforms and nearshore samples. Microcystins were detected in seven survey samples from Owasco Lake, in one survey sample from Seneca Lake, and in one sample each from the open-water monitoring platforms on Owasco and Seneca Lakes that were collected about 7 days before the fall surveys. Microcystin detections were not consistently associated with high cyanobacterial cell counts or cyanotoxin-producing genera. Results from nearshore surveys were comparable to open-water monitoring platforms and discrete nearshore observations in the absence of nearshore or open-water CyanoHABs in these systems during the study. Patterns of nearshore concentrations of nitrate and chlorophyll from nearshore surveys may aid in the identification of areas with localized nitrate loading and shifts in phytoplankton abundance and community composition.

New York

Evaluating the potential of co-located non-petroleum energy resources in the Gulf Coast using play fairwayaAnalysis

Geological resources critical to the energy transition, such as sedimentary geothermal, carbon storage potential, and lithium in brines, commonly struggle for economic feasibility as stand-alone developments but can have greater viability when the potential for more than one of these resources exist within the same reservoir or stacked in different stratigraphic intervals. There may also be instances where development of one resource inhibits development of others and decisions need to be made on how to best prioritize the use of those resources in the subsurface. Subsurface data sets were analyzed and integrated to evaluate the distribution of non-petroleum energy and related resources in the onshore and nearshore U.S. Gulf Coast. Temperature, pressure, brine composition (lithium content), and reservoir quality data for thirty-four depositional units have been compiled and visualized to high-grade areas where multiple resource opportunities likely coexist. For sedimentary geothermal, possible resource areas are defined as low potential (<90°C), moderate potential (90–150°C), and high potential (>150°C). For CO 2 storage, high potential areas exhibit supercritical CO 2 conditions less than 80% of the fracture gradient. Areas with pore pressure between 80% and 100% of the fracture gradient are considered to exhibit moderate potential and areas where the fracture gradient is equal to or greater than 100% are assigned low potential. Lithium resources in brines were defined by lithium concentrations as low potential (<100ppm), moderate potential (100-200ppm), or high potential (>200ppm). Reservoir quality affects the viability of all three of these resources and is evaluated using depositional environment maps of each unit. The resulting play fairway maps can be used for regional scale screening evaluations of these resources and to identify areas of interest where more detailed, prospect-scale studies can be undertaken.

Gulf Coast

Bioconcentration of per- and polyfluoroalkyl substances and precursors in fathead minnow tissues environmentally exposed to aqueous film-forming foam-contaminated waters

Exposure to per- and polyfluoroalkyl substances (PFAS) has been associated with toxicity in wildlife and negative health effects in humans. Decades of fire training activity at Joint Base Cape Cod (MA, USA) incorporated the use of aqueous film-forming foam (AFFF), which resulted in long-term PFAS contamination of sediments, groundwater, and hydrologically connected surface waters. To explore the bioconcentration potential of PFAS in complex environmental mixtures, a mobile laboratory was established to evaluate the bioconcentration of PFAS from AFFF-impacted groundwater by flow-through design. Fathead minnows ( n = 24) were exposed to PFAS in groundwater over a 21-day period and tissue-specific PFAS burdens in liver, kidney, and gonad were derived at three different time points. The ∑PFAS concentrations in groundwater increased from approximately 10,000 ng/L at day 1 to 36,000 ng/L at day 21. The relative abundance of PFAS in liver, kidney, and gonad shifted temporally from majority perfluoroalkyl sulfonamides (FASAs) to perfluoroalkyl sulfonates (PFSAs). By day 21, mean ∑PFAS concentrations in tissues displayed a predominance in the order of liver > kidney > gonad. Generally, bioconcentration factors (BCFs) for FASAs, perfluoroalkyl carboxylates (PFCAs), and fluorotelomer sulfonates (FTS) increased with degree of fluorinated carbon chain length, but this was not evident for PFSAs. Perfluorooctane sulfonamide (FOSA) displayed the highest mean BCF (8700 L/kg) in day 21 kidney. Suspect screening results revealed the presence of several perfluoroalkyl sulfinate and FASA compounds present in groundwater and in liver for which pseudo-bioconcentration factors are also reported. The bioconcentration observed for precursor compounds and PFSA derivatives detected suggests alternative pathways for terminal PFAS exposure in aquatic wildlife and humans.

Environmental Toxicology and Chemistry

Approximate inland extent of saltwater intrusion at the base of the Biscayne aquifer, Miami-Dade County, Florida, 2022

Miami-Dade County is part of a densely populated urban corridor in southeastern Florida. The Biscayne aquifer serves as Miami-Dade County’s primary drinking water source and is characterized by highly permeable karstic limestone and carbonate sand. The aquifer’s coastal location and permeable nature make it susceptible to saltwater intrusion. Monitoring the current inland extent and the rate of movement of the saltwater front in the aquifer can inform management strategies for conserving the long-term sustainability of the county’s water supply. In the 1950s, the U.S. Geological Survey published a map of the inland extent of saltwater intrusion in the Biscayne aquifer and has continued to update this map to monitor changes over time, with the most recent update published in 2018. An updated map has been created showing the approximate inland extent of saltwater intrusion in the Biscayne aquifer in eastern Miami-Dade County in 2022, with the 2018 extent shown for comparison. The inland extent of saltwater intrusion was mapped through the interpretation of borehole electromagnetic induction logs and measurements of chloride and specific conductance in groundwater samples. The location of the saltwater interface at the base of the Biscayne aquifer was represented by the 1,000-milligram-per-liter isochlor. This report describes changes in the location of the saltwater interface from 2018 to 2022. By 2022, the saltwater interface had moved farther inland in both the northern and southern parts of the county, advancing by as much as 0.3 kilometer in the north and up to 0.8 kilometer in the Model Land Area to the south. However, it remained relatively unchanged from its 2018 position in the east-central part of the county.

Florida

Selected water-quality data from the Cedar River and Cedar Rapids well fields, Cedar Rapids, Iowa, 2017–22

The Cedar River alluvial aquifer is the source of drinking water in Cedar Rapids, Iowa. Production wells are completed in the alluvial aquifer approximately 40 to 80 feet below land surface. The City of Cedar Rapids and the U.S. Geological Survey have studied the groundwater-flow system and water quality of the aquifer in the vicinity of Cedar Rapids since 1992. Results of these studies documented hydrologic conditions, water quality, and geochemistry of the alluvial aquifer and interactions with the Cedar River. Water-quality samples were collected for studies involving well field monitoring, trends, source-water protection, groundwater geochemistry, surface-water–groundwater interaction, and pesticides in groundwater and surface water. Water quality was analyzed for dissolved major ions (boron, bromide, calcium, chloride, fluoride, iron, magnesium, manganese, potassium, silica, sodium, sulfate, and total dissolved solids), dissolved nutrients (ammonia as nitrogen, ammonia plus organic nitrogen as nitrogen, nitrite plus nitrate as nitrogen, nitrite as nitrogen, orthophosphate as phosphorus, and phosphorus), dissolved organic carbon, and selected pesticides. Physical characteristics (alkalinity, dissolved oxygen, pH, specific conductance, and water temperature) were measured on site and recorded for each water sample collected. This report presents the results of routine water-quality data-collection activities from October 2017 through September 2022. Methods of data collection, quality assurance, water-quality analyses, and statistical procedures are presented. Data include the results of water-quality analyses from quarterly sampling from monitoring wells, production wells, two water treatment plants, and the Cedar River at Blairs Ferry Road at Palo, Iowa, streamgage (U.S. Geological Survey station number 05464420), as well as monthly nutrient sampling from the Cedar River and Morgan Creek near Covington, Iowa, streamgage (U.S. Geological Survey station number 05464475).

Iowa

Cross section N–N' through the Valley and Ridge province of the southern Appalachian basin, from Greene County, west-central Alabama, to Bibb County, central Alabama

Introduction Geologic cross section N–N′ is the sixth in a series of geologic cross sections constructed by the U.S. Geological Survey to document and improve understanding of the geologic framework and petroleum systems of the Appalachian basin. Cross section N–N′ provides a regional view of the structural and stratigraphic framework of the Appalachian basin in the Valley and Ridge province in western and central Alabama; it spans approximately 69 miles (mi) (111 kilometers [km]). This geologic cross section is a companion to geologic cross sections E–E′ , D–D′ , C–C′ , I–I′ , and A–A′ that are located approximately 350 to 550 mi (563 to 885 km) to the northeast. Cross section N–N' complements earlier geologic cross sections through the Alabama part of the Appalachian basin. Although some of the other cross sections show more structural and stratigraphic detail, they are of more limited extent geographically and stratigraphically. Cross section N–N′ contains information that is useful for evaluating energy resources in the Appalachian basin. Although the Appalachian basin petroleum systems are not shown on the cross section, many of their key elements (such as source rocks, reservoir rocks, seals, and traps) can be inferred from lithologic units, unconformities, and geologic structures shown on the cross section. Other aspects of petroleum systems (such as the timing of petroleum generation and petroleum migration pathways) may be evaluated by burial history, thermal history, and fluid flow models based on what is shown on the cross section. In addition, cross section N–N′ may be used as a reconnaissance tool to identify plausible geologic structures and strata for the subsurface storage of liquid waste or for the sequestration of carbon dioxide.

Alabama

The 2023 US 50-State National Seismic Hazard Model: Overview and implications

The US National Seismic Hazard Model (NSHM) was updated in 2023 for all 50 states using new science on seismicity, fault ruptures, ground motions, and probabilistic techniques to produce a standard of practice for public policy and other engineering applications (defined for return periods greater than ∼475 or less than ∼10,000 years). Changes in 2023 time-independent seismic hazard (both increases and decreases compared to previous NSHMs) are substantial because the new model considers more data and updated earthquake rupture forecasts and ground-motion components. In developing the 2023 model, we tried to apply best available or applicable science based on advice of co-authors, more than 50 reviewers, and hundreds of hazard scientists and end-users, who attended public workshops and provided technical inputs. The hazard assessment incorporates new catalogs, declustering algorithms, gridded seismicity models, magnitude-scaling equations, fault-based structural and deformation models, multi-fault earthquake rupture forecast models, semi-empirical and simulation-based ground-motion models, and site amplification models conditioned on shear-wave velocities of the upper 30 m of soil and deeper sedimentary basin structures. Seismic hazard calculations yield hazard curves at hundreds of thousands of sites, ground-motion maps, uniform-hazard response spectra, and disaggregations developed for pseudo-spectral accelerations at 21 oscillator periods and two peak parameters, Modified Mercalli Intensity, and 8 site classes required by building codes and other public policy applications. Tests show the new model is consistent with past ShakeMap intensity observations. Sensitivity and uncertainty assessments ensure resulting ground motions are compatible with known hazard information and highlight the range and causes of variability in ground motions. We produce several impact products including building seismic design criteria, intensity maps, planning scenarios, and engineering risk assessments showing the potential physical and social impacts. These applications provide a basis for assessing, planning, and mitigating the effects of future earthquakes.

Earthquake Spectra

Assessing environmental drivers of denitrification in restored riverine floodplains

Restoration of impaired floodplains is an increasingly prevalent strategy for alleviating water quality concerns and reducing downstream flooding at watershed scales. Floodplains temporarily store water and slow flow velocity to promote sedimentation during overbank flooding and remove inorganic nitrogen from floodwater and groundwater via denitrification. Evaluating the impacts of different restoration strategies on denitrification can inform more strategic investments into floodplain modifications that improve water quality outcomes. Our research investigates how denitrification rates in floodplains respond to environmental factors that are actionable from an engineering perspective through design and water resources management. We seasonally measured soil denitrification enzyme activity and various environmental characteristics in 4 floodplains with different restoration design and management approaches at the confluence of the Wabash and Tippecanoe Rivers in Indiana, United States. Our results showed that denitrification rates in an agricultural floodplain were significantly lower than in restored floodplains with native vegetation. Certain soil conditions characteristic of floodplain wetlands were associated with higher denitrification, particularly elevated total nitrogen, moisture, silt, and organic matter contents. Vegetation species composition was correlated with denitrification rates. This link may reflect the direct effects of vegetation on soil conditions, such as supplying labile organic carbon, or indirect effects, such as vegetation acting as an indicator of hydrologic regime and land use. Denitrification seasonally varied, peaking in winter when nitrate supply from rivers draining agricultural watersheds in the region is also high. Substrate limitation of soil denitrification enzyme activity was most significant during the summer when overbank flooding, which replenishes soil nitrogen stocks, rarely occurs. Our findings indicate that denitrification capacity will likely be maximized in riverine floodplains that are restored as wetlands with diverse native vegetation and enhanced hydrologic connectivity. Such restoration activities promote higher denitrification rates via elevated moisture, fine sediment deposition, and soil organic matter.

Tippecanoe River, Wabash River

Phase 1 technical implementation plan for the expansion of the ShakeAlert earthquake early warning system to Alaska

Executive Summary The conference report accompanying the fiscal year (FY) 2022 Consolidated Appropriations Act (Public Law 117–103) for the U.S. Department of the Interior and related agencies directed the U.S. Geological Survey (USGS) to “work with the State of Alaska to develop an implementation plan to be completed within two years in order to put ShakeAlert/Earthquake Early Warning in Alaska” (p. 29). Congress included $1 million in the FY 2022 appropriation to conduct this effort. The USGS Earthquake Hazards Program, along with partner organizations, has developed the ShakeAlert earthquake early warning (EEW) system for the West Coast, which currently operates in California, Oregon, and Washington. The purpose of the system and its alert delivery partners is to reduce the impact of earthquakes and save lives and property by delivering ShakeAlert-powered alerts that are transmitted to the public via mass notification technologies, and by providing more detailed data streams to institutional users and commercial service providers to trigger automated, user-specific, protective actions. ShakeAlert was designed in such a way that it could be expanded to other U.S. regions with high earthquake risk, after the build-out of seismic and geodetic networks to support ShakeAlert in a specified region is completed and the necessary funding is secured for long-term operation and maintenance. When an earthquake occurs, seismic waves radiate from the rupturing fault like waves on a pond. It is these waves that people feel as earthquake shaking and that can cause damage to structures. Using networks of ground-motion sensors and sophisticated computer algorithms, ShakeAlert can detect an earthquake seconds after it begins, calculate its location and magnitude, and estimate the resulting intensity of shaking. Early warnings of impending shaking are then sent to people and systems that may experience damaging shaking, allowing them to take appropriate protective actions. Depending on the user’s distance from the earthquake, alerts may be delivered before, during, or after the arrival of strong shaking. There will almost always be a region near the earthquake epicenter where alerts arrive after damaging shaking has begun. The ShakeAlert system updates its ground-motion estimates as an earthquake grows larger. In response to the FY 2022 congressional direction, the USGS worked with the State of Alaska to devise this implementation plan for ShakeAlert expansion to Alaska. The USGS engaged with the Alaska Division of Homeland Security and Emergency Management (DHS&EM) and the Alaska Division of Geological and Geophysical Surveys (DGGS). A cooperative agreement was awarded to the Alaska Earthquake Center (AEC) at the University of Alaska Fairbanks (UAF) for their contributions to the plan and their work coordinating with other networks in Alaska. The USGS engaged with the Alaska Seismic Hazards Safety Commission (ASHSC) throughout the process. The USGS also held a series of Alaska stakeholder engagements. The process of developing the implementation plan was facilitated by contracted staff from Corner Alliance, which is a government consulting firm. This implementation plan describes the details and estimates the costs for a Phase 1 expansion of the ShakeAlert system to Alaska. A geographically limited Phase 1 goal was chosen that covers the highest risk and most populated areas of Alaska. The areas proposed encompass the State’s main population centers and 90 percent of the State’s population. This Phase 1 design is considered very challenging and ambitious from the viewpoint of network operators. The lessons learned if this plan is implemented could be used to consider subsequent phases to expand EEW beyond Phase 1 in Alaska in the future. ShakeAlert is built on the foundation of the sensor networks and data processing infrastructure of the USGS-led Advanced National Seismic System (ANSS). This implementation plan calls for a total of 450 high-quality, real-time EEW-capable ANSS seismic stations in Alaska: 270 new stations, 160 upgraded stations, and 20 existing stations. These seismic station numbers are based on a station spacing of 10 kilometers (km) in urban areas, 20 km in seismic source areas that endanger population centers, and 40 km in other areas. The associated costs also include support for some EEW-capable global navigation satellite system (GNSS) stations, with a focus on improving warnings for large subduction zone earthquakes. For effective EEW, ShakeAlert requires low-latency, high-availability, robust telemetry links to deliver continuous, real-time data from field stations to the data centers. The Alaska data processing hardware infrastructure would follow the general design for fail-safe operation that is used for the ShakeAlert system on the West Coast. The ShakeAlert architecture uses two independent layers: the production layer for earthquake processing and the alert layer to make alerting decisions and serve alerts to users. This implementation plan includes two geographically separated data centers in Alaska, each with two fully independent production and alert layers using the same system design developed for the West Coast. As of March 2024, the ShakeAlert system is at version 3.0.1, with more advanced versions in the development and testing pipeline. ShakeAlert originally used two algorithms to determine the location and magnitude of earthquakes using seismic data. A third algorithm that can calculate very large magnitudes of very large earthquakes with geodetic data was added in March 2024. ShakeAlert publishes several data and alert products to meet the needs of different users. All messages include the location of the earthquake, either as a point or a line, and its magnitude. Ground-shaking estimates are published in two forms, as ground-motion contours and a map grid. Providing adequate warning time for strong shaking (the “target threshold”) requires sending alerts at a threshold lower than that strong shaking level (the “alert threshold”). The thresholds for public alerting in Alaska would be a joint USGS and State decision. To have the greatest benefit, ShakeAlert-powered alerts would be delivered to institutional users and individuals by all practical pathways. The USGS alert layer can support thousands of institutional users and alert redistributors, but the USGS does not have the mission nor the infrastructure and expertise to perform mass notifications to the public or implement automatic actions for end users of the alerts. To meet this need, ShakeAlert recruits private sector “technology enablers” that have the necessary expertise to develop end-user implementations using EEW alerts with the goal of stimulating an EEW industry. Earthquake early warning alerts are useless if people do not know how to respond to them. Although the alert messages include instructions about what to do (drop, cover, and hold on), alerts are more effective if people have been trained in advance. Messages about ShakeAlert’s capabilities, limitations, and benefits could be integrated with existing earthquake education programs, including State-run programs. Therefore, ShakeAlert would coordinate with both public and private partners and stakeholders through various partnerships and agreements to accomplish consistent and ongoing public earthquake hazard education. The estimated capital cost of completing the computing infrastructure and sensor networks for the Phase 1 ShakeAlert expansion to Alaska is approximately $66 million in 2024 dollars. The annual operation and maintenance cost of the completed system is estimated to be $12 million per year in 2024 dollars when fully built out.

Alaska

Utilization of multiple geochronology techniques to constrain the age of laterization and mineralization of the world-class Mount Weld rare earth element deposit, Western Australia

Pervasive chemical weathering on stable cratons may form thick regoliths and elemental enrichment, but constraining the age of regolith formation is challenging. In this study we utilize multiple geochronological techniques on different minerals from the world-class Mount Weld rare earth element (REE) deposit, formed by lateritic weathering of a carbonatite, to constrain the age of formation and provide insight into landscape evolution. The oldest dates, ca. 100 to 38 Ma, are from Lu-Hf dating of churchite [HREE(PO 4 )·2(H 2 O)], a heavy REE phosphate mineral. Growth bands on individual minerals show a younging outwards. 40 Ar/ 39 Ar geochronology of cryptomelane [K(Mn 4+ ,Mn 2+ )₈O₁₆] yielded dates from ca. 40 to 27 Ma. Similarly, (U-Th)/He geochronology of goethite [FeO(OH)] yielded dates ranging from ca. 45 to 19 Ma. Integrating results into regional constraints, suggests 1) churchite formed by mineral saturation in a karst-like setting below the water table from ca. 100 to 40 Ma, 2) with minor uplift and erosion, cryptomelane and goethite formed at or near the water table between ca. 45 and 19 Ma, 3) after ca. 15 to 10 Ma chemical weathering within the profile had ended. Other studies document that the region experienced minimal uplift and a wet, warm climate from ca. 100 Ma to 15 Ma. These conditions and the high carbonate content of the carbonatite promote extensive chemical weathering, a deep weathering profile, and the preservation of the weathered section. This study highlights the use of multiple geochronological techniques utilizing different minerals to provide insight into how laterites form and to constrain the timing and history of the formation of this important mineral deposit.

Western Australia

Characterization of change in tree cover state and condition over the conterminous United States

Variability in the effects of disturbances and extreme climate events can lead to changes in tree cover over time, including partial or complete loss, with diverse ecological consequences. It is therefore critical to identify in space and time the change processes that lead to tree cover change. Studies of change are often hampered by the lack of data capable of consistently detecting different types of change. Using the Landsat satellite record to create a long time-series of land cover and land cover change, the U.S. Geological Survey Land Change Monitoring Assessment and Projection (LCMAP) project has made an annual time series of land cover across the conterminous United States for the period 1985 to 2018. Multiple LCMAP products analyzed together with map validation reference plots provide a robust basis for understanding tree cover change. In LCMAP (Collection 1.2), annual change detection is based on harmonic model breaks calculated at each Landsat pixel from the Continuous Change Detection and Classification (CCDC) algorithm. The results showed that the majority of CCDC harmonic model breaks (signifying change) indicated partial tree cover loss (associated with management practices such as tree cover thinning) as compared to complete tree cover loss (associated with practices like clearcut harvest or fire disturbance). Substantially fewer occurrences of complete tree cover loss were associated with change in land cover state. The area of annual tree cover change increased after the late 1990s and stayed high for the rest of the study period. The reference data showed that tree harvest dominated across the conterminous United States. The majority of tree cover change occurred in evergreen forests. Large estimates of disturbance-related tree cover change indicated that tree cover loss may have previously been underreported due to omission of partial tree cover loss in prior studies. This has considerable implications for forest carbon accounting along with tracking ecosystem goods and services.

Forests