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Assessment of nonpoint source chemical loading potential to watersheds containing uranium waste dumps and human health hazards associated with uranium exploration and mining, Red, White, and Fry Canyons, southeastern Utah, 2007

During May, June, and July 2007, 58 solid-phase samples were collected from abandoned uranium mine waste dumps, background sites, and adjacent streambeds in Red, White, and Fry Canyons in southeastern Utah. The objectives of this sampling program were to (1) assess the nonpoint-source chemical loading potential to ephemeral and perennial drainage basins from uranium waste dumps and (2) assess potential effects on human health due to recreational activities on and around uranium waste dumps on Bureau of Land Management property. Uranium waste-dump samples were collected using solid-phase sampling protocols. After collection, solid-phase samples were homogenized and extracted in the laboratory using a leaching procedure. Filtered (0.45 micron) water samples were obtained from the field leaching procedure and were analyzed for major and trace elements at the Inductively Coupled Plasma-Mass Spectrometry Metals Analysis Laboratory at the University of Utah. A subset of the solid-phase samples also were digested with strong acids and analyzed for major ions and trace elements at the U.S. Geological Survey Geologic Division Laboratory in Denver, Colorado. For the initial ranking of chemical loading potential for uranium waste dumps, results of leachate analyses were compared with existing aquatic-life and drinking-water-quality standards. To assess potential effects on human health, solid-phase digestion values for uranium were compared to soil screening levels (SSL) computed using the computer model RESRAD 6.5 for a probable concentration of radium. One or more chemical constituents exceeded aquatic life and drinking-water-quality standards in approximately 64 percent (29/45) of the leachate samples extracted from uranium waste dumps. Most of the uranium waste dump sites with elevated trace-element concentrations in leachates were located in Red Canyon. Approximately 69 percent (31/45) of the strong acid digestible soil concentration values were greater than a calculated SSL. Uranium waste dump sites with elevated leachate and total digestible concentrations may need to be further investigated to determine the most appropriate remediation method.

Utah

Water-quality characteristics and ground water quantity of the Fraser River watershed, Grand County, Colorado, 1998-2001

The U.S. Geological Survey, in cooperation with the Grand County Board of County Commissioners, conducted a 4-year study to assess ground- and surface-water-quality conditions and ground-water quantity in the 302-square-mile Fraser River watershed in north-central Colorado. The Fraser River flows north about 28 miles from the headwaters near the Continental Divide, through the towns of Winter Park, Fraser, Tabernash, and Granby, and is one of the major tributaries to the Upper Colorado River. Increasing urban development, as well as the seasonal influx of tourists, is placing more demands on the water resources in the Fraser River watershed. A ground-water sampling network of 11 wells was established to represent different aquifer systems (alluvial, Troublesome Formation, Precambrian granite), land uses (urban, nonurban), and areas with or without individual septic disposal system use. The well network was sampled for ground-water quality on a semiannual basis from August 1998 through September 2001. The sampling included field properties and the collection of water samples for analysis of major ions, trace elements, nutrients, dissolved organic carbon, bacteria, methylene blue active substances, and radon-222. One surface-water site, on the Fraser River just downstream from the town of Tabernash, Colorado, was sampled bimonthly from August 1998 through September 2001 to assess the cumulative effects of natural and human processes on water quality in the upper part of the Fraser River watershed. Surface-water-quality sampling included field properties and the collection of water-quality samples for analysis of major ions, trace elements, nutrients, organic carbon, and bacteria. Ground water was a calcium-bicarbonate type water and is suitable as a drinking-water, domestic, municipal, industrial, and irrigation source. In general, no widespread ground-water-quality problems were indicated. All pH values and concentrations of dissolved solids, chloride, fluoride, sulfate, nitrite, and nitrate in the ground-water samples met or were substantially less than U.S. Environmental Protection Agency drinking-water standards and health advisories or State of Colorado water-quality standards. Federal standards for turbidity and concentrations of iron, manganese, methylene blue active substances, and radon-222 were not met in water samples from at least one well. The only ground-water-quality concern assessed by this study is radon-222, which was detected in all radon- analyzed samples from 10 wells at levels exceeding the proposed U.S. Environmental Protection Agency drinking-water standard of 300 picocuries per liter. Concentrations of chloride, magnesium, and sulfate were statistically different (higher) in ground-water samples from wells completed in the alluvial aquifer, urbanized areas, and areas with individual septic disposal system use than those from wells completed in the Troublesome Formation, nonurban areas, and areas without individual septic disposal system use. Dissolved organic carbon concentrations were statistically higher in ground-water samples from wells completed in the alluvial aquifer and areas without individual septic disposal system use than those from wells completed in the Troublesome Formation and areas with individual septic disposal system use. Differences in dissolved organic-carbon concentrations between the latter category and areas without septic systems likely had no environmental significance. Surface water at the site Fraser River below Crooked Creek at Tabernash was a calcium-bicarbonate type water and is suitable as a drinking-water, residential, commercial, and irrigation resource. All pH values and concentrations of dissolved oxygen were within the State of Colorado instream water-quality standards, and all concentrations of chloride, sulfate, iron, manganese, un-ionized ammonia, nitrite, nitrate, and fecal coliform bacteria met State standards. Seasonal changes in the values or conc

Colorado

Burrowing mayflies as indicators of ecosystem health: Status of populations in two western Lake Superior embayments

The U.S. Environmental Protection Agency and Environment Canada are supporting the development of indicators of ecosystem health that can be used to report on progress in restoring and maintaining the Great Lakes ecosystem, as called for in the Great Lakes Water Quality Agreement between the United States and Canada. One indicator under development for Great Lakes mesotrophic environments is based on burrowing mayflies ( Hexagenia : Ephemeroptera: Ephemeridae). In this paper, we report the results of a benthic survey in spring 2002 to determine the status of nymphal populations of Hexagenia in two western Lake Superior embayments, the St. Louis River estuary, an area with significant water-use impairments, and Chequamegon Bay, an area with no known water-use impairments. Ponar grab samples collected throughout these embayments showed nymphs were generally abundant in finely particulate, cohesive substrate (clay or mixtures of clay and sand) in both embayments. However, in the St. Louis River estuary nymphs were absent in those preferred substrates at 11 stations in the eastern portion of St. Louis Bay and the adjoining northwestern portion of the Duluth-Superior Harbor, where the sediments were variously contaminated with visible amounts of taconite pellets, paint chips, oil, or combusted coal waste (clinkers). Our results suggest that human activities have rendered those portions of the St. Louis River estuary unsuitable for habitation by Hexagenia nymphs and we recommend that trend monitoring of the nymphal population there be conducted to permit reporting on progress in restoring and maintaining the health and integrity of this Great Lakes ecosystem embayment, consistent with the intent of the Great Lakes Water Quality Agreement.

Minnesota, Wisconsin

Illegal dumping of oil and gas wastewater alters arid soil microbial communities

The Permian Basin, underlying southeast New Mexico and west Texas, is one of the most productive oil and gas (OG) provinces in the United States. Oil and gas production yields large volumes of wastewater with complex chemistries, and the environmental health risks posed by these OG wastewaters on sensitive desert ecosystems are poorly understood. Starting in November 2017, 39 illegal dumps, as defined by federal and state regulations, of OG wastewater were identified in southeastern New Mexico, releasing ~600,000 L of fluid onto dryland soils. To evaluate the impacts of these releases, we analyzed changes in soil geochemistry and microbial community composition by comparing soils from within OG wastewater dump-affected samples to unaffected zones. We observed significant changes in soil geochemistry for all dump-affected compared with control samples, reflecting the residual salts and hydrocarbons from the OG-wastewater release (e.g., enriched in sodium, chloride, and bromide). Microbial community structure significantly ( P < 0.01) differed between dump and control zones, with soils from dump areas having significantly ( P < 0.01) lower alpha diversity and differences in phylogenetic composition. Dump-affected soil samples showed an increase in halophilic and halotolerant taxa, including members of the Marinobacteraceae, Halomonadaceae, and Halobacteroidaceae, suggesting that the high salinity of the dumped OG wastewater was exerting a strong selective pressure on microbial community structure. Taxa with high similarity to known hydrocarbon-degrading organisms were also detected in the dump-affected soil samples. Overall, this study demonstrates the potential for OG wastewater exposure to change the geochemistry and microbial community dynamics of arid soils.

Applied and Environmental Microbiology

Pesticides and nitrate in groundwater underlying citrus croplands, Lake Wales Ridge, central Florida, 1999-2005.

This report summarizes pesticide and nitrate (as nitrogen) results from quarterly sampling of 31 surficial-aquifer wells in the Lake Wales Ridge Monitoring Network during April 1999 through January 2005. The wells, located adjacent to citrus orchards and used for monitoring only, were generally screened (sampled) within 5 to 40 feet of the water table. Of the 44 citrus pesticides and pesticide degradates analyzed, 17 were detected in groundwater samples. Parent pesticides and degradates detected in quarterly groundwater samples, ordered by frequency of detection, included norflurazon, demethyl norflurazon, simazine, diuron, bromacil, aldicarb sulfone, aldicarb sulfoxide, deisopropylatrazine (DIA), imidacloprid, metalaxyl, thiazopyr monoacid, oxamyl, and aldicarb. Reconnaissance sampling of five Network wells yielded detection of four additional pesticide degradates (hydroxysimazine, didealkylatrazine, deisopropylhydroxyatrazine, and hydroxyatrazine). The highest median concentration values per well, based on samples collected during the 1999–2005 period (n=14 to 24 samples per well), included 3.05 µg/L (micrograms per liter) (simazine), 3.90 µg/L (diuron), 6.30 µg/L (aldicarb sulfone), 6.85 µg/L (aldicarb sulfoxide), 22.0 µg/L (demethyl norflurazon), 25.0 µg/ (norflurazon), 89 µg/ (bromacil), and 25.5 mg/L (milligrams per liter) (nitrate). Nitrate concentrations exceeded the 10 mg/L (as nitrogen) drinking water standard in one or more groundwater samples from 28 of the wells, and the median nitrate concentration among these wells was 14 mg/L. Sampled groundwater pesticide concentrations exceeded Florida’s health-guidance benchmarks for aldicarb sulfoxide and aldicarb sulfone (4 wells), the sum of aldicarb and its degradates (6 wells), simazine (2 wells), the sum of simazine and DIA (3 wells), diuron (2 wells), bromacil (1 well), and the sum of norflurazon and demethyl norflurazon (1 well). The magnitude of fluctuations in groundwater pesticide concentrations varied between wells and between pesticide compounds. Of the 10 pesticide compounds detected at sufficient frequency to assess temporal variability in quarterly sampling records, median values of the relative interquartile range (ratio of the interquartile range to the median) among wells typically ranged from about 100 to 150 percent. The relative interquartile range of pesticide concentrations at individual wells could be much higher, sometimes exceeding 200 to 500 percent. No distinct spatial patterns were apparent among median pesticide concentrations in sampled wells; nitrate concentrations tended to be greater in samples from wells in the northern part of the study area.

Florida

Optimization of salt marsh management at the Moosehorn National Wildlife Refuge, Maine, through use of structured decision making

Structured decision making is a systematic, transparent process for improving the quality of complex decisions by identifying measurable management objectives and feasible management actions; predicting the potential consequences of management actions relative to the stated objectives; and selecting a course of action that maximizes the total benefit achieved and balances tradeoffs among objectives. The U.S. Geological Survey, in cooperation with the U.S. Fish and Wildlife Service, applied an existing, regional framework for structured decision making to develop a prototype tool for optimizing tidal marsh management decisions at the Moosehorn National Wildlife Refuge in Maine. Refuge biologists, refuge managers, and research scientists identified multiple potential management actions to improve the ecological integrity of four marsh management units within the refuge, totaling about 13 hectares, and estimated the outcomes of each action in terms of performance metrics associated with each management objective. Value functions previously developed at the regional level were used to transform metric scores to a common utility scale, and utilities were summed to produce a single score representing the total management benefit that could be accrued from each potential management action. Constrained optimization was used to identify the set of management actions, one per marsh management unit, that could maximize total management benefits at different cost constraints at the refuge scale. Results indicated that, for the objectives and actions considered here, total management benefits may increase consistently up to $ 1,000, and may continue to increase at a lower rate with further expenditures. Potential management actions in optimal portfolios at total costs less than or equal to $ 1,000 included improving nesting habitat for Ammodramus nelsoni (Nelson’s sparrow) or restoring hydrologic connections to the upper marsh in one marsh management unit (Hobart Stream West). The potential management benefits were derived from expected increases in the density of nekton and of spiders (as an indicator of trophic health). The prototype presented here does not resolve management decisions; rather, it provides a framework for decision making at the Moosehorn National Wildlife Refuge that can be updated for implementation as new data and information become available. Insights from this process may also be useful to inform future habitat management planning at the refuge.

Maine

Estimation of Constituent Concentrations, Loads, and Yields in Streams of Johnson County, Northeast Kansas, Using Continuous Water-Quality Monitoring and Regression Models, October 2002 through December 2006

Johnson County is one of the most rapidly developing counties in Kansas. Population growth and expanding urban land use affect the quality of county streams, which are important for human and environmental health, water supply, recreation, and aesthetic value. This report describes estimates of streamflow and constituent concentrations, loads, and yields in relation to watershed characteristics in five Johnson County streams using continuous in-stream sensor measurements. Specific conductance, pH, water temperature, turbidity, and dissolved oxygen were monitored in five watersheds from October 2002 through December 2006. These continuous data were used in conjunction with discrete water samples to develop regression models for continuously estimating concentrations of other constituents. Continuous regression-based concentrations were estimated for suspended sediment, total suspended solids, dissolved solids and selected major ions, nutrients (nitrogen and phosphorus species), and fecal-indicator bacteria. Continuous daily, monthly, seasonal, and annual loads were calculated from concentration estimates and streamflow. The data are used to describe differences in concentrations, loads, and yields and to explain these differences relative to watershed characteristics. Water quality at the five monitoring sites varied according to hydrologic conditions; contributing drainage area; land use (including degree of urbanization); relative contributions from point and nonpoint constituent sources; and human activity within each watershed. Dissolved oxygen (DO) concentrations were less than the Kansas aquatic-life-support criterion of 5.0 mg/L less than 10 percent of the time at all sites except Indian Creek, which had DO concentrations less than the criterion about 15 percent of the time. Concentrations of suspended sediment, chloride (winter only), indicator bacteria, and pesticides were substantially larger during periods of increased streamflow. Suspended-sediment concentration was nearly always largest at the Mill Creek site. The Mill Creek watershed is undergoing rapid development that likely contributed to larger sustained sediment concentrations. During most of the time, the smallest sediment concentrations occurred at the Indian Creek site, the most urban of the monitored sites, likely because most of the streamflow originates from wastewater-treatment facilities located just upstream from the monitoring site. However, estimated annual suspended-sediment load and yield were largest annually at the Indian Creek site because of substantial contributions during storm runoff. At least 90 percent of the total annual sediment load in 2005?06 at all five monitoring sites occurred in less than 2 percent of the time, generally associated with large storm runoff. About 50 percent of the 2005 sediment load at the Blue River site occurred during a single 3-day storm, the equivalent of less than 1 percent of the time. Suspended-sediment concentration is statistically related to other water-quality constituents, and these relations have potential implications for implementation of best management practices because, if sediment concentrations are decreased, concentrations of sediment-associated constituents such as suspended solids, some nutrients, and bacteria will also likely decrease. Chloride concentrations were largest at the Indian and Mill Creek sites, the two most urban stream sites which also are most affected by road-salt runoff and wastewater-treatment-facility discharges. Two chloride runoff occurrences in January?February 2005 accounted for 19 percent of the total chloride load in Indian Creek in 2005. Escherichia coli density at the Indian Creek site was nearly always largest of the five sites with a median density more than double that of any other site and 15 times the density at the Blue River site which is primarily nonurban. More than 97 percent of the fecal coliform bacteria load at the Indian Creek site and near the B

Scientific Investigations Report

Lead exposure in waterfowl before contoxic shot requirements: A nationwide study, 1983−1986

Before implementing nontoxic shot requirements for hunting waterfowl and American coots Fulica americana in the United States in 1991, the U.S. Fish and Wildlife Service monitored lead poisoning in waterfowl on federal and state wildlife hunting areas during 1983-1986. Federal and state collaborators collected gizzards and livers from 9,029 hunter-killed waterfowl (10 species of dabbling ducks Anatinae , 9 diving ducks Aythyinae , 5 geese Anserinae , and tundra swans Cygnus columbianus ) across the four flyways. At the U.S. Fish and Wildlife Service National Wildlife Health Center, Madison, Wisconsin, waterfowl gizzards were examined for ingested lead and nontoxic shot and livers were analyzed for lead concentrations. Diving ducks had the greatest frequency (8.7%) of one or more ingested lead shot, followed by dabbling ducks (5.5%) and geese (1.3%). No ingested shot were found in tundra swans. The frequency of elevated (≥ 2.0 mg/kg wet weight) liver lead concentrations was also greatest in diving ducks, followed by dabbling ducks and geese. Within each species group, the frequency of elevated liver lead concentrations was greater than ingested lead shot, an indication that lead shot ingestion alone underrepresents lead exposure. Thus, lead in the liver may remain elevated after the erosion and excretion of lead pellets from the gizzard. Our results provide historical baseline data and summarize a nationwide study of lead exposure, using both ingested lead shot and liver lead concentrations, in waterfowl in the United States before the implementation of nontoxic shot regulations in 1991. These data can be compared with previous studies of lead exposure in waterfowl, as well as current and future assessments to evaluate the success of nontoxic shot regulations nationwide and specifically within previously sampled waterfowl management areas.

Journal of Fish and Wildlife Management

Response of fish populations to natural channel design restoration in streams of the Catskill Mountains, New York

Many streams and rivers throughout North America have been extensively straightened, widened, and hardened since the middle 1800s, but related effects on aquatic ecosystems have seldom been monitored, described, or published. Beginning in the early 1990s, reach-level restoration efforts began to base projects on natural channel design (NCD) techniques and Rosgen's (1994b, 1996) river classification system in an effort to duplicate or mimic stable reference reach geomorphology. Four reaches in three streams of the Catskill Mountains, New York, were restored from 2000 to 2002 using NCD techniques to decrease bed and bank erosion rates, decrease sediment loads, and improve water quality. The effects of restoration on the health of fish assemblages were assessed through a before-after, control-impact (BACI) study design to quantify the net changes in population and community indices at treatment reaches relative to index changes at unaltered reference reaches from 1999 to 2004. After restoration, community richness and biomass at treatment reaches increased by more than one-third. Changes in fish communities were caused mainly by shifts in dominant species populations; fish community biomass and total fish abundance were generally dominated by daces or daces and sculpins before restoration and by one or more salmonid species after restoration. Density and biomass of eastern blacknose dace Rhinichthys atratulus, longnose dace R. cataractae, and slimy sculpin Cottus cognatus did not change appreciably, whereas net salmonid density and biomass increased substantially after restoration. These changes were driven primarily by large increases in populations of brown trout Salmo trutta. The findings demonstrate that the structure, function, and ultimately the health of resident fish populations and communities can be improved, at least over the short term, through NCD restoration in perturbed streams of the Catskill Mountains. ?? Copyright by the American Fisheries Society 2008.

North American Journal of Fisheries Management

Advancements in hydrochemistry mapping: methods and application to groundwater arsenic and iron concentrations in Varanasi, Uttar Pradesh, India

The area east of Varanasi is one of numerous places along the watershed of the Ganges River with groundwater concentrations of arsenic surpassing the maximum value of 10 parts per billion (ppb) recommended by the World Health Organization in drinking water. Here we apply geostatistics and compositional data analysis for the mapping of arsenic and iron to help in understanding the conditions leading to the occurrence of elevated level of arsenic in groundwater. The methodology allows for displaying concentrations of arsenic and iron as maps consistent with the limited information from 95 water wells across an area of approximately 210 km 2 ; visualization of the uncertainty associated with the sampling; and summary of the findings in the form of probability maps. For thousands of years, Varanasi has been on the erosional side in a meander of the river that is free of arsenic values above 10 ppb. Maps reveal two anomalies of high arsenic concentrations on the depositional side of the valley, which has started seeing urban development. The methodology using geostatistics combined with compositional data analysis is completely general, so this study could be used as a prototype for hydrochemistry mapping in other areas.

Uttar Pradesh

Temporal variation in fish mercury concentrations within lakes from the western Aleutian Archipelago, Alaska

We assessed temporal variation in mercury (Hg) concentrations of threespine stickleback ( Gasterosteus aculeatus ) from Agattu Island, Aleutian Archipelago, Alaska. Total Hg concentrations in whole-bodied stickleback were measured at two-week intervals from two sites in each of two lakes from June 1 to August 10, 2011 during the time period when lakes were ice-free. Across all sites and sampling events, stickleback Hg concentrations ranged from 0.37–1.07 µg/g dry weight (dw), with a mean (± SE) of 0.55±0.01 µg/g dw. Mean fish Hg concentrations declined by 9% during the study period, from 0.57±0.01 µg/g dw in early June to 0.52±0.01 µg/g dw in mid-August. Mean fish Hg concentrations were 6% higher in Loon Lake (0.56±0.01 µg/g dw) than in Lake 696 (0.53±0.01 µg/g dw), and 4% higher in males (0.56±0.01 µg/g dw) than in females (0.54±0.01 µg/g dw). Loon Lake was distinguished from Lake 696 by the presence of piscivorous waterbirds during the breeding season. Mercury concentrations in stickleback from Agattu Island were higher than would be expected for an area without known point sources of Hg pollution, and high enough to be of concern to the health of piscivorous wildlife.

Alaska

The southern megalopolis: using the past to predict the future of urban sprawl in the Southeast U.S.

The future health of ecosystems is arguably as dependent on urban sprawl as it is on human-caused climatic warming. Urban sprawl strongly impacts the urban ecosystems it creates and the natural and agro-ecosystems that it displaces and fragments. Here, we project urban sprawl changes for the next 50 years for the fast-growing Southeast U.S. Previous studies have focused on modeling population density, but the urban extent is arguably as important as population density per se in terms of its ecological and conservation impacts. We develop simulations using the SLEUTH urban growth model that complement population-driven models but focus on spatial pattern and extent. To better capture the reach of low-density suburban development, we extend the capabilities of SLEUTH by incorporating street-network information. Our simulations point to a future in which the extent of urbanization in the Southeast is projected to increase by 101% to 192%. Our results highlight areas where ecosystem fragmentation is likely, and serve as a benchmark to explore the challenging tradeoffs between ecosystem health, economic growth and cultural desires.

PLoS ONE

Occurrence and distribution of selected contaminants in public drinking-water supplies in the surficial aquifer in Delaware

Water samples were collected from August through November 2000 from 30 randomly selected public drinking-water supply wells screened in the unconfined aquifer in Delaware, and analyzed to assess the occurrence and distribution of selected pesticide compounds, volatile organic compounds, major inorganic ions, and nutrients. Water from a subset of 10 wells was sampled and analyzed for radium and radon. The average age of ground water entering the well screens in all the wells was determined to be generally less than 20 years. Low concentrations of pesticide compounds and volatile organic compounds were detected throughout the State of Delaware, with several compounds often detected in each water sample. Pesticide and metabolite (pesticide degradation products) concentrations were generally less than 1 microgram per liter, and were detected in sam-ples from 27 of 30 wells. Of the 45 pesticides and 13 metabolites analyzed, 19 compounds (13 pesticides and 6 metabolites) were detected in at least 1 of the 30 samples. Desethylatrazine, alachlor ethane sulfonic acid, metolachlor ethane sulfonic acid, metolachlor, and atrazine were the most frequently detected pesticide compounds, and were present in at least half the samples. None of the pesticide detections was above the U.S. Environmental Protection Agency's Primary Maximum Contaminant Levels or Health Advisories. Volatile organic compounds also were present at low concentrations (generally less than 1 microgram per liter) in samples from all 30 wells. Of the 85 volatile organic com-pounds analyzed, 34 compounds were detected in at least 1 of the 30 samples. Chloroform, tetrachloroethene, and methyl tert-butyl ether were the most frequently detected volatile organic compounds, and were found in at least half the samples. None of the volatile organic compound detections was above U.S. Environmental Protection Agency's Primary Maximum Contaminant Levels or Health Advisories. A few samples contained compounds with concentrations above the U.S. Environmental Protection Agency's Primary Maximum Contaminant Levels or Secondary Maximum Contaminant Levels for inorganic compounds and radionuclides. One sample out of 30 contained a concentration of nitrite plus nitrate above the U.S. Environmental Protection Agency's Primary Maximum Contaminant Level of 10 milligrams per liter as nitrogen. Iron and manganese concentrations above the U.S. Environmental Protection Agency's Secondary Maximum Contaminant Levels were found in 7 of 30 ground-water samples, most of them from Sussex County. In the 10 wells sampled for radionuclides, only one sample had detectable levels of radium-224 and -226, and another sample contained detectable levels of radium-228; both of these samples also had detectable gross-alpha and gross-beta activities. None of these activities were above the U.S. Environ-mental Protection Agency's Primary Maximum Contaminant Levels or Secondary Maximum Contaminant Levels. Radon was detected in all 10 samples, but was above the current U.S. Environmental Protection Agency's proposed Primary Maximum Contaminant Level of 300 picocuries per liter in only one sample.

Open-File Report

Variations in Community Exposure and Sensitivity to Tsunami Hazards in the State of Hawai'i

Hawai`i has experienced numerous destructive tsunamis and the potential for future events threatens the safety and economic well being of its coastal communities. Although tsunami-evacuation zones have been delineated, what is in these areas and how communities have chosen to develop within them has not been documented. A community-level vulnerability assessment using geographic-information-system tools was conducted to describe tsunami-prone landscapes on the Hawaiian coast and to document variations in land cover, demographics, economic assets, and critical facilities among 65 communities. Results indicate that the Hawai`i tsunami-evacuation zone contains approximately 56,678 residents (five percent of the total population), 67,113 employees (eleven percent of the State labor force), and 50,174 average daily visitors to hotels (44 percent of the State total). With regards to economic conditions, the tsunami-evacuation zone contains 5,779 businesses that generate $10.1 billion in annual sales volume (both eleven percent of State totals), and tax parcels with a combined total value of $36.1 billion (18 percent of the State total). Although occupancy values are not known for each facility, the tsunami-evacuation zone also contains numerous dependent-population facilities (for example, child-day-care facilities and schools), public venues (for example, religious organizations and parks) and critical facilities (for example, fire stations). The residential population in tsunami-prone areas is racially diverse, with most residents identifying themselves as White, Asian, or Native Hawaiian and Other Pacific Islander, either alone or in combination with one or more race. Fifty-one percent of the households in the tsunami-evacuation zone are renter occupied. The employee population in the tsunami-evacuation zone is largely in accommodation and food services, health services, and retail-trade sectors. Results indicate that community vulnerability, described here by exposure (the amount of assets in tsunami-prone areas) and sensitivity (the relative percentage of assets in tsunami-prone areas) varies considerably among 65 coastal communities in Hawai`i. Honolulu has the highest exposure, Punalu`u has the highest sensitivity, and Ka`anapali has the highest combination of exposure and sensitivity to tsunamis. Results also indicate that the level of community-asset exposure to tsunamis is not determined by the amount of a community's land that is in tsunami-evacuation zones. Community sensitivity, however, is related to the percentage of a community's land that is in the tsunami-prone areas. This report will further the dialogue on societal risk to tsunami hazards in Hawai`i and help identify future preparedness, mitigation, response, and recovery planning needs within coastal communities and economic sectors of the State of Hawai`i.

Scientific Investigations Report

Trends in suspended-sediment concentration at selected stream sites in Kansas, 1970–2002

Knowledge of erosion, transport, and deposition of sediment relative to streams and impoundments is important to those involved directly or indirectly in the development and management of water resources. Monitoring the quantity of sediment in streams and impoundments is important because: (1) sediment may degrade the water quality of streams for such uses as municipal water supply, (2) sediment is detrimental to the health of some species of aquatic animals and plants, and (3) accumulation of sediment in water-supply impoundments decreases the amount of storage and, therefore, water available for users. One of the objectives of the Kansas Water Plan is to reduce the amount of sediment in Kansas streams by 2010. During the last 30 years, millions of dollars have been spent in Kansas watersheds to reduce sediment transport to streams. Because the last evaluation of trends in suspended-sediment concentrations in Kansas was completed in 1985, 14 sediment sampling sites that represent 10 of the 12 major river basins in Kansas were reestablished in 2000. The purpose of this report is to present the results of time-trend analyses at the reestablished sediment data-collection sites for the period of about 1970–2002 and to evaluate changes in the watersheds that may explain the trends. Time-trend tests for 13 of 14 sediment sampling sites in Kansas for the period from about 1970 to 2002 indicated that 3 of the 13 sites tested had statistically significant decreasing suspended-sediment concentrations; however, only 2 sites, Walnut River at Winfield and Elk River at Elk Falls, had trends that were statistically significant at the 0.05 probability level. Increasing suspended-sediment concentrations were indicated at three sites although none were statistically significant at the 0.05 probability level. Samples from five of the six sampling sites located upstream from reservoirs indicated decreasing suspended-sediment concentrations. Watershed impoundments located in the respective river basins may contribute to the decreasing suspended-sediment trends exhibited at most of the sampling sites because the impoundments are designed to trap sediment. Both sites that exhibited statistically significant decreasing suspended-sediment concentrations have a large number of watershed impoundments located in their respective drainage basins. The relation between percentage of the watershed affected by impoundments and trend in suspended-sediment concentration for 11 sites indicated that, as the number of impoundments in the watershed increases, suspended-sediment concentration decreases. Other conservation practices, such as terracing of farm fields and contour farming, also may contribute to the reduced suspended-sediment concentrations if their use has increased during the period of analysis. Regression models were developed for 13 of 14 sediment sampling sites in Kansas and can be used to estimate suspended-sediment concentration if the range in stream discharge for which they were developed is not exceeded and if time trends in suspended-sediment concentrations are not significant. For those sites that had a statistically significant trend in suspended-sediment concentration, a second regression model was developed using samples collected during 2000–02. Past and current studies by the U.S. Geological Survey have shown that regression models can be developed between in-stream measurements of turbidity and laboratory-analyzed sediment samples. Regression models were developed for the relations between discharge and suspended-sediment concentration and turbidity and suspended-sediment concentration for 10 sediment sampling sites using samples collected during 2000–02.

Kansas

Occurrence of fecal coliform bacteria in selected streams in Wyoming, 1990-99

The U.S. Geological Survey (USGS), in cooperation with the Wyoming Department of Environmental Quality (WDEQ), is collecting water samples for analysis of fecal coliform bacteria at 18 stream sites as part of a statewide network. Contamination by bacteria of fecal origin in streams where contact recreation is a designated water use is a concern because of potential public-health risk from the presence of enteric pathogens. Fecal coliform concentrations are temporally and spatially variable in Wyoming streams-concentrations ranged from less than 1 to 45,000 colonies per 100 milliliters of water during 1990-99. Fecal coliform concentrations were less than the water-quality criterion of 400 colonies per 100 milliliters in 83 percent of the samples, indicating fecal coliform contamination is not a widespread problem in these Wyoming streams. However, 14 of the 18 monitoring sites had at least one sample in which the fecal coliform concentration exceeded 400 colonies per 100 milliliters at some time during the 10-year period. Fecal coliform concentrations generally are higher during April through September than during October through March. The higher concentrations coincide with the time period when the public-health risk is higher because summer months are when contact recreation use is more likely occurring. Fecal coliform concentrations were positively correlated with discharge and stream temperature and generally were negatively correlated with pH, specific conductance, and dissolved oxygen.

Wyoming

Status of whitebarkpine in the Greater Yellowstone Ecosystem: A step-trend analysis comparing 2004-2007 to 2008-2011

Whitebark pine (Pinus albicaulis) is a foundation and keystone species in upper subalpine environments of the northern Rocky Mountains that strongly influences the biodiversity and productivity of high-elevation ecosystems (Tomback et al. 2001, Ellison et al. 2005). Throughout its historic range, whitebark pine has decreased significantly as a major component of high-elevation forests. As a result, it is critical to understand the challenges to whitebark pine—not only at the tree and stand level, but also as these factors influence the distribution of whitebark pine across the Greater Yellowstone Ecosystem (GYE). In 2003, the National Park Service (NPS) Greater Yellowstone Inventory & Monitoring Network identified whitebark pine as one of twelve significant natural resource indicators or vital signs to monitor (Jean et al. 2005, Fancy et al. 2009) and initiated a long-term, collaborative monitoring program. Partners in this effort include the U.S. Geological Survey, U.S. Forest Service, and Montana State University with representatives from each comprising the Greater Yellowstone Whitebark Pine Monitoring Working Group. The objectives of the monitoring program are to assess trends in (1) the proportion of live, whitebark pine trees (>1.4-m tall) infected with white pine blister rust (blister rust); (2) to document blister rust infection severity by the occurrence and location of persisting and new infections; (3) to determine mortality of whitebark pine trees and describe potential factors contributing to the death of trees; and (4) to assess the multiple components of the recruitment of understory whitebark pine into the reproductive population. In this report we summarize the past eight years (2004-2011) of whitebark pine status and trend monitoring in the GYE. Our study area encompasses six national forests (NF), two national parks (NP), as well as state and private lands in portions of Wyoming, Montana, and Idaho; this area is collectively described as the GYE here and in other studies. The sampling design is a probabilistic, twostage cluster design with stands of whitebark pine as the primary units and 10x50 m belt transects as the secondary units. Primary sampling units (stands) were selected randomly from a sample frame of approximately 10,770 mapped pure and mixed whitebark pine stands ≥2.0 hectares in the GYE (Dixon 1997, Landenburger 2012). From 2004 through 2007 (monitoring transect establishment or initial time-step), we established 176 permanent belt transects (secondary sampling units=176) in 150 whitebark pine stands and permanently marked approximately 4,740 individual trees >1.4 m tall to monitor long-term changes in blister rust infection and survival rates. Between 2008 and 2011 (revisit time-step), these same 176 transects were surveyed and again all previously tagged trees were observed for changes in blister rust infection and survival status. Objective 1. Using a combined ratio estimator, we estimated the proportion of live trees infected in the GYE in the initial time-step (2004-2007) to be 0.22 (0.031 SE). Following the completion of all surveys in the revisit time-step (2008-2011), we estimated the proportion of live trees infected with white pine blister rust as 0.23 (0.028 SE; Table 2). We detected no significant change in the proportion of trees infected in the GYE between the two time-steps. Objective 2. We documented blister rust canker locations as occurring in the canopy or bole. We compared changes in canker position between the initial time-step (2004-2007) and the revisit time-step (2008-2011) in order to assess changes in infection severity. This analysis included the 3,795 trees tagged during the initial time-step that were located and documented as alive at the end of the revisit time-step. At the end of the revisit time-step, we found 1,217 trees infected with blister rust. This includes the 287 newly tagged trees in the revisit time step of which 14 had documented infections. Of these 1,217 trees, 780 trees were infected with blister rust in both time steps. Trees with only canopy cankers made up approximately 43% (519 trees) of the total number of trees infected with blister rust at the end of the revisit time-step, while trees with only bole cankers comprised 20% (252 trees), and those with both canopy and bole cankers included 37% (446 trees) of the infected sample. A bole infection is considered to be more consequential than a canopy canker, as it compromises not only the overall longevity of the tree, but its functional capacity for reproductive output as well (Kendall and Arno 1990, Campbell and Antos 2000, McDonald and Hoff 2001, Schwandt and Kegley 2004). In addition to infection location, we also documented infection transition between the canopy and bole. Of the 780 live trees that were infected with blister rust in both time-steps, approximately 31% (242) maintained canopy cankers and 36% (281) retained bole infections at the end of the revisit time-step. Infection transition from canopy to bole occurred in 30% (234) of the revisit time-step trees while 3% (23) transitioned from bole to canopy infections during this period. Objective 3. To determine whitebark pine mortality, we resurveyed all belt transects to reassess the life status of permanently tagged trees >1.4 m tall. We compared the total number of live tagged trees recorded during monitoring transect establishment to the total number of resurveyed dead tagged trees recorded during the revisit time-step and identified all potential mortality-influencing conditions (blister rust, mountain pine beetle, fire and other). By the end of the revisit time-step, we observed a total of 975 dead tagged whitebark pine trees; using a ratio estimator, this represents a loss of approximately 20% (SE=4.35%) of the original live tagged tree population (GYWPMWG 2012). Objective 4. To investigate the proportion of live, reproducing tagged trees, we divided the total number of positively identified cone-bearing trees by the total number of live trees in the tagged tree sample at the end of the revisit time-step. To approximate the average density of recruitment trees per stand, trees ≤1.4 m tall were summed by stand (within the 500 m² transect area) and divided by the total number of stands. Reproducing trees made up approximately 24% (996 trees) of the total live tagged population at the end of the revisit time-step. Differentiating between whitebark pine and limber pine seedlings or saplings is problematic given the absence of cones or cone scars. Therefore, understory summaries as presented in this report may include individuals of both species when they are sympatric in a stand. The average density of small trees ≤1.4 m tall was 53 understory trees per 500 m². Raw counts of these understory individuals ranged from 0-635 small trees per belt transect. In addition, a total of 287 trees were added to the tagged tree population by the end of 2011. These newly tagged trees were individuals that upon subsequent revisits had reached a height of >1.4 m tall and subsequently added to the sample. Throughout the past decade in the GYE, monitoring has helped document shifts in whitebark pine forests; whitebark pine stands have been impacted by insect, pathogen, wildland fire, and other disturbance events. Blister rust infection is ubiquitous throughout the ecosystem and infection proportions are variable across the region. And while we have documented mortality of whitebark pine, we have also recorded considerable recruitment. We provide this first step-trend report as a quantifiable baseline for understanding the state of whitebark pine in the GYE. Many aspects of whitebark pine health are highly variable across the range of its distribution in the GYE. Through sustained implementation of the monitoring program, we will continue efforts to document and quantify whitebark pine forest dynamics as they arise under periodic upsurges in insect, pathogen, fire episodes, and climatic events in the GYE. Since its inception, this monitoring program perseveres as one of the only sustained longterm efforts conducted in the GYE with a singular purpose to track the health and status of this prominent keystone species.

Natural Resource Technical Report

Retrospective study of the diagnostic criteria in a lead-poisoning survey of waterfowl

Between 1983 and 1986 the National Wildlife Health Center (NWHC) conducted a nationwide study of lead poisoning of waterfowl from federal and state refuges. This survey was done to assist in identifying zones with lead-poisoning problems. One thousand forty one moribund or dead waterfowl were collected and examined. The presence or absence of 13 gross lesions selected as indicators of lead poisoning and 3 lesions indicating body condition was recorded. Lead-poisoning diagnoses were based on the finding of at least 6 8 ppm (wet weight) lead in the liver and either lead shot in the gizzard content or at least one convincing gross lesion indicative of lead poisoning. Four hundred and twenty-one of these waterfowl were diagnosed as lead-poisoned. The NWHC survey provided a comprehensive basis for estimating the sensitivities, specificities, and likelihood ratios of the gross lesions of lead poisoning and the associated hepatic lead concentrations for several species of waterfowl. Some of the 13 defined gross lesions were more common than others; frequencies ranged from 3% to 80% in the 421 lead-poisoned waterfowl. The most reliable indicators of lead poisoning were impactions of the upper alimentary tract, submandibular edema, myocardial necrosis, and biliary discoloration of the liver. Each of the 13 lesions occurred more frequently in the lead-poisoned birds, but each of the lesions also occurred in waterfowl that died of other causes. The number of lead shot present in a bird?s gizzard was only weakly correlated with its hepatic lead concentration; however, this weak correlation may have been adequate to account for differences in hepatic lead concentrations among species, once the weights of the species were taken into account. Although lead-poisoned ducks tended to have higher hepatic mean lead concentrations than did lead-poisoned geese or swans, the differences were probably a result of a greater dose of shot per body weight than to kinetic differences between species. Hepatic lead concentrations were independent of age and sex. Ninety-five percent of waterfowl diagnosed as lead-poisoned had hepatic lead concentrations of at least 38 ppm, dry weight (10 ppm, wet weight). Fewer than 1% of the waterfowl that died of other causes had a concentration that high. This 5th percentile, of 38 ppm dry weight (10 ppm wet weight), is a defensible criterion for identifying lead-poisoned waterfowl when interpreting hepatic lead concentrations in the absence of pathological observations.

Archives of Environmental Contamination and Toxico