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Exploring and mitigating plague for One Health purposes

Purpose of Review In 2020, the Appropriations Committee for the U.S. House of Representatives directed the CDC to develop a national One Health framework to combat zoonotic diseases, including sylvatic plague, which is caused by the flea-borne bacterium Yersinia pestis . This review builds upon that multisectoral objective. We aim to increase awareness of Y. pestis and to highlight examples of plague mitigation for One Health purposes (i.e., to achieve optimal health outcomes for people, animals, plants, and their shared environment). We draw primarily upon examples from the USA, but also discuss research from Madagascar and Uganda where relevant, as Y. pestis has emerged as a zoonotic threat in those foci. Recent Findings Historically, the bulk of plague research has been directed at the disease in humans. This is not surprising, given that Y. pestis is a scourge of human history. Nevertheless, the ecology of Y. pestis is inextricably linked to other mammals and fleas under natural conditions. Accumulating evidence demonstrates Y. pestis is an unrelenting threat to multiple ecosystems, where the bacterium is capable of significantly reducing native species abundance and diversity while altering competitive and trophic relationships, food web connections, and nutrient cycles. In doing so, Y. pestis transforms ecosystems, causing “shifting baselines syndrome” in humans, where there is a gradual shift in the accepted norms for the condition of the natural environment. Eradication of Y. pestis in nature is difficult to impossible, but effective mitigation is achievable; we discuss flea vector control and One Health implications in this context. Summary There is an acute need to rapidly expand research on Y. pestis , across multiple host and flea species and varied ecosystems of the Western US and abroad, for human and environmental health purposes. The fate of many wildlife species hangs in the balance, and the implications for humans are profound in some regions. Collaborative multisectoral research is needed to define the scope of the problem in each epidemiological context and to identify, refine, and implement appropriate and effective mitigation practices.

Current Tropical Medicine Reports

Intrinsic germanium detector used in borehole sonde for uranium exploration

A borehole sonde (~1.7 m long; 7.3 cm diameter) using a 200 mm 2 planar intrinsic germanium detector, mounted in a cryostat cooled by removable canisters of frozen propane, has been constructed and tested. The sonde is especially useful in measuring X- and low-energy gamma-ray spectra (40–400 keV). Laboratory tests in an artificial borehole facility indicate its potential for in-situ uranium analyses in boreholes irrespective of the state of equilibrium in the uranium series. Both natural gamma-ray and neutron-activation gamma-ray spectra have been measured with the sonde. Although the neutron-activation technique yields greater sensitivity, improvements being made in the resolution and efficiency of intrinsic germanium detectors suggest that it will soon be possible to use a similar sonde in the passive mode for measurement of uranium in a borehole down to about 0.1% with acceptable accuracy. Using a similar detector and neutron activation, the sonde can be used to measure uranium down to 0.01%.

Nuclear Instruments and Methods

Aerial infrared surveys of Reykjanes and Torfajökull thermal areas, Iceland, with a section on cost of exploration surveys

In 1966 and 1968 aerial infrared surveys were conducted over 10 of 13 high-temperature thermal areas in Iceland. The surveys were made with an airborne scanner system, utilizing radiation in the 4.5–5.5 μm wavelength band. Supplementary ground geological studies were made in the Reykjanes and Torfajökull thermal areas to interpret features depicted on the infrared imagery and to relate zones of high heat flux to tectonic structure. In the Reykjanes area in southwestern Iceland a shallow ground temperature map was prepared for temperatures at a depth of 0.5 meters; comparison of this map with the infrared imagery reveals some striking similarities. It appears that aerial infrared surveys outline the surface thermal patterns of high-temperature areas and aid in relating these patterns to possible geological structures controlling the upflow of hot water. Amplitude-slicing techniques applied to the magnetically taped airborne scanner data permit an estimate to be made of the natural heat output on the basis of size of area and specific radiance. In addition to their value in preliminary studies of high-temperature areas, infrared surveys conducted at regular intervals over thermal area under exploitation can provide valuable data on changes that occur in surface manifestations with time. e.

Reykjanes, Torfajokull

Hydrogeochemistry in the Yukon-Tanana Upland region of east-central Alaska: Possible exploration tool for porphyry-style deposits

A hydrogeochemical study using high resolution ICP-MS was undertaken at the Taurus and other porphyry Cu-Mo(-Au) occurrences and Ag-Au-Cu (+/- Pb, Zn) occurrences with epithermal-style characteristics in the Yukon-Tanana upland region of eastern Alaska. Surface water samples were collected from 30 sites on creeks that drain known deposits and occurrences and surrounding presumably unmineralized areas. Water samples for the entire ∼9 km length of McCord Creek, which drains the Taurus deposit, and those from streams draining the areas at and near the Bluff and Dennison porphyry occurrences have high conductivity values (492 to 1250 μS/cm) and consistently high concentrations of B (3-250 μg/L), Co (2.3 to 42 μg/L), Mn (339 to 4750 μg/L), Re (0.012 to 0.1 μg/L), and SO 4 2- (>200 mg/L), all of which are well above the median value for this data set and significantly greater than concentrations in water samples from the unmineralized areas. These are the best pathfinder elements specifically for porphyry style deposits because most of them are not anomalous in waters near epithermal occurrences. Copper concentrations are high (up to 115 μg/L) in some low-pH water samples from McCord Creek and drainages around Bluff, and a few near neutral pH waters have high molybdenum (>1 μg/L), but neither element is consistently anomalous in close vicinity to the porphyry occurrences, possibly due to a metal-poor, sulfide-poor leached cap (average of ∼50 m) that overlies supergene and hypogene mineralized zones and is the dominant rock at surface. High concentrations of Bi and/or As occur in many waters associated with mineralized areas, particularly the Bluff and Dennison occurrences. In general, the element associations related to porphyry deposits reflect the deposit mineralogy, as well as size of the footprint related to alteration and mineralization.

Alaska

Chemostratigraphy of the Cretaceous Hue Shale in Arctic Alaska: Exploring paleoceanographic controls on trace element enrichment, organic matter accumulation, and source-rock evolution

We document chemostratigraphy in an outcrop of late Albian to early Campanian (∼103–82 Ma) marine source rocks to better understand paleoenvironmental controls on trace element (TE) enrichment and organic matter accumulation in the distal Colville foreland basin of Arctic Alaska and how those drivers are linked to arc volcanism and successions of Cretaceous oceanographic and climatic biogeochemical events. This unique, 113-m-thick section of Cretaceous Hue Shale deposited during a series of previously undocumented Arctic Cretaceous oceanic anoxic events (Lease et al., 2024) is the only known exposure of thermally immature (0.48–0.52% R o , random vitrinite reflectance) Hue Shale in Arctic Alaska. Strata comprise mainly clay-rich mudstone with elevated total organic carbon (TOC) and hydrogen index values reaching 26.3 wt% (mean = 7.5 wt%) and 689 mg hydrocarbon (HC)/g TOC (mean = 385 mg HC/g TOC), respectively. Maceral composition consists predominantly of fluorescent amorphous organic matter, with abundant brightly fluorescent alginite, including Tasmanites , acritarchs, and Leiosphaeridia . Discrete layers of volcanic ash (preserved as bentonite) are present throughout the section and provide quantitative age control based on U–Pb dates. Chemostratigraphic trends are investigated to advance our understanding of local oceanographic conditions and controls on stratigraphic and temporal heterogeneity of Brookian source rocks. Concurrent sedimentary enrichment in Mo, U, V, Pb, and Cu across the Albian–Cenomanian boundary of the exposed basal gamma-ray zone, may reflect anoxic to euxinic benthic redox conditions favoring organic matter accumulation and preservation. Fluctuating degrees of anoxia-euxinia are inferred throughout the overlying Hue Shale succession, reflected by varying patterns of TE enrichment and TE–TOC covariation. Elevated C org /P molar ratios (>250) across most of the section, with several values exceeding 690, signify that enhanced biological productivity is sustained throughout deposition. Enhanced productivity, recorded by both C org /P and excess Ba, also parallels increases in source rock richness (elevated TOC and S 2 values) during the late Albian–early Cenomanian and late Cenomanian – Turonian. Enhanced productivity and variations in oceanic circulation/stratification likely both drove changes in benthic redox conditions that favored organic carbon accumulation and preservation. Increased continental arc volcanism (e.g., Okhotsk–Chukotka volcanic belt) and High Arctic Large Igneous Province magmatic eruptions throughout the Cretaceous, inferred to have influenced nutrient cycling and local aqueous nutrient availability, also have been invoked as potential drivers of organic carbon burial and source-rock development across the sedimentary sequence. Results presented here document the organic-rich and oil-prone source-rock quality of the Hue Shale in the distal part of the Colville foreland basin and bolster the potential for a Cretaceous petroleum system beneath the eastern North Slope.

Alaska

Monitoring multiple species: Estimating state variables and exploring the efficacy of a monitoring program

Monitoring programs have the potential to identify population declines and differentiate among the possible cause(s) of these declines. Recent criticisms regarding the design of monitoring programs have highlighted a failure to clearly state objectives and to address detectability and spatial sampling issues. Here, we incorporate these criticisms to design an efficient monitoring program whose goals are to determine environmental factors which influence the current distribution and measure change in distributions over time for a suite of amphibians. In designing the study we (1) specified a priori factors that may relate to occupancy, extinction, and colonization probabilities and (2) used the data collected (incorporating detectability) to address our scientific questions and adjust our sampling protocols. Our results highlight the role of wetland hydroperiod and other local covariates in the probability of amphibian occupancy. There was a change in overall occupancy probabilities for most species over the first three years of monitoring. Most colonization and extinction estimates were constant over time (years) and space (among wetlands), with one notable exception: local extinction probabilities for Rana clamitans were lower for wetlands with longer hydroperiods. We used information from the target system to generate scenarios of population change and gauge the ability of the current sampling to meet monitoring goals. Our results highlight the limitations of the current sampling design, emphasizing the need for long-term efforts, with periodic re-evaluation of the program in a framework that can inform management decisions.

Biological Conservation

Exploring the persistence of sorted bedforms on the inner-shelf of Wrightsville Beach, North Carolina

Geological studies offshore of Wrightsville Beach, North Carolina reveal subtle large-scale regions of coarse sand with gravel and shell hash (widths between 100 and 200 m and negative relief of ∼1 m) that trend obliquely to the coast. It was previously suggested that these regions serve as conduits for sand exchange between the shoreface and inner shelf during storm-associated downwelling. Consequently they were classified as rippled scour depressions. More recently, the role of alongshore flows and self-organization as a result of inhibited settling of fine sand has been discussed. In this study, 45 days of near-bed current measurements were analyzed using benthic boundary layer and sediment transport models to examine the role of along- and cross-shore flows in driving sediment transport at this site. The wind climate was found to be a dominant influence on near-bed flows. Six distinct sediment transport events were recognized. During these events, sediment transport models show that bedload transport is directed mainly in the cross-shore direction, while suspended sediments are directed alongshore to the southwest. Current observations during these sediment transport events provide no evidence of cross-shore sediment transport caused by steady downwelling currents. Instead, benthic boundary layer model results are used to show that differences in bed roughness between the coarse areas of the seabed within the “rippled scour depressions” and the finer areas of the inner shelf are more pronounced during increasingly energetic wave and current conditions. The enhanced difference in roughness results in increased turbulence intensities over coarse regions inhibiting the deposition of the fine sand that is resuspended over the shelf during these events relative to finer areas over the shelf. Findings from this study contribute to explaining the observed long-term persistence of these features.

North Carolina

Exploring relationships among stream health, human well-being, and demographics in Virginia, USA

Quantification of empirical relationships between ecosystem health and human well-being is uncommon at broad spatial scales. We used public data for Virginia (USA) counties to examine pairwise correlations among two indicators of stream health, thirteen indicators of human well-being, and four demographic metrics. Our indicators of stream health included the Virginia Stream Condition Index (VSCI) and the percentage of stream kilometers with a fish consumption advisory (%FCA); these measures are inversely related. VSCI and %FCA were correlated with some indicators of human health, safety and security, and living standards, as well as with some demographic metrics. VSCI was most strongly correlated (positively) with the percentage of a county’s population self-identifying as White; %FCA was most strongly correlated (positively) with overall mortality rate (number of deaths per 100,000 people). This exploratory study highlights the need for future multidisciplinary, multiscale studies to characterize toxicological, epidemiological, socioeconomic, and political linkages – including causal mechanisms – between ecosystem health and human well-being.

Virginia

Enumeration of Mars years and seasons since the beginning of telescopic exploration

A clarification for the enumeration of Mars Years prior to 1955 is presented, along with a table providing the Julian dates associated with Ls = 0° for Mars Years -183 (beginning of the telescopic study of Mars) to 100. A practical algorithm for computing Ls as a function of the Julian Date is provided. No new science results are presented

Icarus

Exploring the disparate tectonic manifestations of lithospheric shortening on ocean worlds: Enceladus and Europa

The moons Europa and Enceladus both have water-ice dominated shells tens of kilometers thick overlying global oceans. Both moons are tidally heated and have a high rock mass fractions relative to other moons in their system, and both have youthful, tectonically deformed surfaces. Despite their many commonalities, the surfaces of the two moons bear little resemblance to one another. Europa’s tectonics are dominated by extensional features, including wide bands that imply extremely large surface strains. Features formed by the requisite lithospheric shortening accompanying such extension are largely absent, but long-wavelength folding may have occurred in some locations. Enceladus’ tectonics include features formed in both extension and contraction. Unlike Europa, folds, fold and thrust belts, and isolated thrust faults are plentiful there. In this investigation we use numerical simulations of the shortening of ice lithospheres to bring new insight into why two moons with so much in common appear so different from each other. We find that the temperature structure of the lithosphere controls the style of deformation that results from shortening. Warm conditions resulting from higher surface temperatures and heat fluxes produce folding, which is essentially a viscous response to shortening. Cold temperatures resulting from lower surface temperatures and heat fluxes results in faulting, which is a brittle response. Intermediate conditions can lead to complex combinations of folding and faulting. We hypothesize that Europa’s surface is generally warm enough for shortening to be accommodated by subtle folds that are difficult to detect in the sparse topography data currently available, whereas Enceladus’ colder temperatures favor a combination of folding and faulting. We argue that the dependence of tectonic style on lithospheric temperature self-consistently explains the pattern of tectonics observed across Enceladus, and we emphasize that non-rigid deformation (folding) is not inconsistent with the emerging paradigm of plate tectonics on Europa.

Icarus

Exploring the barriers for people taking protective actions during the 2012 and 2015 New Zealand shakeout drills

To reduce future earthquake injuries and casualties, it is important that people understand how their behavior, during and immediately following earthquake shaking, exposes them to increased risk of injury or death. Research confirms that protective actions can reduce injuries and that prior training can help prepare people to take appropriate actions. In this paper, we examine barriers to participation in the ShakeOut drills in New Zealand. Through citizen science research, volunteers observed people performing the drills in 2012 and 2015. Observers reported how long it took to perform the drill and why they thought some people may not have completed it. Our findings illustrate that children, elderly, and those with both mental and physical disabilities struggled with the drill. Furthermore, embarrassment was a reported leading cause for non-participation; we recommend more inclusive messaging to address potential causes of embarrassment.

International Journal of Disaster Risk Reduction

A spatial exploration of informal trail networks within Great Falls Park, VA

Informal (visitor-created) trails represent a threat to the natural resources of protected natural areas around the globe. These trails can remove vegetation, displace wildlife, alter hydrology, alter habitat, spread invasive species, and fragment landscapes. This study examines informal and formal trails within Great Falls Park, VA, a sub-unit of the George Washington Memorial Parkway, managed by the U.S. National Park Service. This study sought to answer three specific questions: 1) Are the physical characteristics and topographic alignments of informal trails significantly different from formal trails, 2) Can landscape fragmentation metrics be used to summarize the relative impacts of formal and informal trail networks on a protected natural area? and 3) What can we learn from examining the spatial distribution of the informal trails within protected natural areas? Statistical comparisons between formal and informal trails in this park indicate that informal trails have less sustainable topographic alignments than their formal counterparts. Spatial summaries of the lineal and areal extent and fragmentation associated with the trail networks by park management zones compare park management goals to the assessed attributes. Hot spot analyses highlight areas of high trail density within the park and findings provide insights regarding potential causes for development of dense informal trail networks.

Virginia

Exploring how vessel activity influences the soundscape at a navigation lock on the Mississippi River

Vessel sound is now globally recognized as a significant and pervasive pollutant to aquatic life. However, compared to marine environments, there is a paucity of data on sound emitted by vessel activity in freshwater habitats. The Upper Mississippi River (UMR) is home to a diverse array of aquatic life as well as being a key route for barge transportation with 29 locks and dams. In this study, passive acoustic monitoring was conducted at Lock and Dam 19 near Keokuk, Iowa, on the UMR between 20 June – August 28, 2019 to coincide with peak navigation use. There was a significant increase in median sound pressure level (SPL; 50–12,000 Hz) recorded during vessel passages (123 dB re. 1μPa for recreational vessels and 137 dB re. 1μPa for commercial vessels) compared to median background levels (111 dB re. 1μPa). Results provide information on the ambient soundscape at a navigation lock, providing a baseline essential for future studies gauging the effect of anthropogenic sound on aquatic life. Lock 19 has also been identified as a potential site for acoustic deterrent deployment to prevent invasive fish movements. The results of this study can help determine the sound level or frequency deterrents would need to emit, to avoid those currently produced during vessel passage.

Illinois, Iowa

Exploring potential effects of cormorant predation on the fish community in Saginaw Bay, Lake Huron

Stakeholders and fishery managers expressed concern that double-crested cormorant Phalacrocorax auritus predation may be a factor in the recent poor survival of yellow perch Perca flavescens in Saginaw Bay. We quantified cormorant diets from two nesting colonies in Saginaw Bay during April–September in 2013 and 2014, with special emphasis on impacts to yellow perch. Cormorants ( n = 691) were collected when returning to colonies after foraging. Stomachs were removed and preserved in the field. Diet items were identified, enumerated, and measured ( n = 23.373). Cormorant diets from Saginaw Bay indicate a heavy reliance on round goby and Notropis species as prey during the breeding season, consistent with other areas of the Great Lakes where round goby and cormorants coincide. Respectively, the three most common prey species observed by number (%) and biomass (%) pooled across years and sites were round goby Neogobius melanostomus (56.6%, 42.1%), emerald shiner Notropis antherinoides (25.2%, 12.5%), and yellow perch (8.0%, 14.1%). Diet composition was more variable at Spoils Island than at Little Charity Island. Overall cormorant consumption (estimated using cormorant consumption demand rates) of yellow perch was compared to walleye consumption. Cormorant consumption of age-1 yellow perch was 13–17% as much as mean walleye consumption of yellow perch in 2013 and 8–11% in 2014. The cumulative effects of walleye and spring cormorant predation likely represent a recruitment bottleneck for yellow perch in Saginaw Bay. Future studies determining age-specific abundance of yellow perch would facilitate better determination of cormorant predation significance.

Lake Huron, Saginaw Bay

Zooplankton dynamics in a Great Lakes connecting channel: Exploring the seasonal composition within the St. Clair-Detroit River System

The connecting channels linking the Laurentian Great Lakes provide important migration routes, spawning grounds, and nursery habitat for fish, but their role as conduits between lakes for zooplankton is less understood. To address this knowledge gap in the St. Clair–Detroit River System (SCDRS), a comprehensive survey of crustacean zooplankton was performed in both riverine and lacustrine habitats from spring to fall 2014, providing the first system-wide assessment of zooplankton in the SCDRS. Zooplankton density and biomass were greatest in northern reaches of the system (southern Lake Huron and the St. Clair River) and decreased downstream towards Lake Erie. The composition of zooplankton also changed moving downstream, transitioning from a community dominated by calanoid copepods, to more cyclopoids and cladocerans in the Detroit River, and to cladocerans dominant in western Lake Erie. Coincidentally, species richness increased as sampling progressed downstream, and we estimated that our single-year sampling regime identified ~88% of potential taxa. Other species assemblages have responded positively to recent water quality and habitat restoration efforts in the SCDRS, and this survey of the zooplankton community provides benchmark information necessary to assess its response to continued recovery. In addition, information regarding the lower trophic levels of the system is integral to understanding recruitment of ecologically and economically valuable fish species targeted for recovery in the SCDRS.

St. Clair-Detroit River System

Tracking persistent declines in suspended sediment in the Lower Mississippi and Atchafalaya Rivers, 1992–2021: Harnessing WRTDSplus to characterize longitudinally varying trends and explore connections to streamflow

Suspended sediment (SS) continues a century-long decline in the Lower Mississippi and Atchafalaya Rivers, United States. In this study, we use the WRTDSplus model to estimate concentrations and loads for total, fine (<0.0625 millimeter (mm)), and coarse (≥0.0625 mm) SS for 11 sites. This extension of the Weighted Regressions on Time, Discharge, and Season (WRTDS) model allows a fourth explanatory variable in the model formulation. We incorporated hysteresis terms for most models based on a residual analysis, which allowed for the identification of decreased flushing over time at some sites. Total, fine, and coarse SS concentrations and loads decreased at all sites over two trend periods (water years (WY) 1992–2021 and WY 2012–2021). Declines were largely due to changes in fine SS (mud and silt) but decreases in coarse SS (sands) were also widespread. On average, recent declines are more severe in the Lower Mississippi River below the Old River Control Complex (ORCC, −3.7 mg per liter per year (mg/L/yr)) compared to the Atchafalaya River (−2.0 mg/L/yr), although there is longitudinal variability within each river. The reach below the ORCC is a net SS sink, leading to complex temporal changes for the sites in this area. Streamflows (low, moderate, and high) have increased over these periods, with the last decade being particularly wet. Increasing streamflow and decreasing SS, with little evidence of amelioration, may influence spillway operations during floods, sediment diversion construction and operation, coastal restoration efforts, and aquatic health.

Louisiana, Mississippi

Application of an updated atmospheric model to explore volcano infrasound propagation and detection in Alaska

Winds and temperature gradients greatly affect the long-range propagation of infrasound . The spatio-temporal variability of these parameters must therefore be accurately characterized to correctly interpret recorded infrasound at long distances, specifically to differentiate between source and propagation effects. Here we present the first results of an open source reanalysis model, termed Alaska Volcano Observatory Ground-to-Space (AVO-G2S), constructed to accurately characterize the atmosphere and model long-range infrasound propagation from volcanic eruptions in Alaska. We select a number of case studies to examine recent eruptions of Alaskan volcanoes whose ash emissions posed a threat to air traffic, including the two most recent eruptions of Pavlof Volcano and two typical explosions from Cleveland Volcano. Strong tropospheric ducting and low noise at the station during the 21 July 2015 explosion of Cleveland Volcano led to an automated detection of the explosion at an infrasound array 992 km away, whereas low signal-to-noise ratio for the 6 November 2014 Cleveland Volcano explosion helps explain the non-detection in real-time of a predicted strong stratospheric arrival. For the November 2014 Pavlof eruption, discrepancies between local seismic data and a distal infrasound array 460 km away cannot be solely explained by changes in atmospheric conditions, though some features of the complex propagation predictions follow the trends in long-range infrasound signals. The most recent eruption of Pavlof Volcano in March 2016 shows minimal changes in propagation conditions throughout the eruption and therefore indicates that the signals detected at long-range primarily reflect source processes. These results show how detailed examination of the acoustic propagation conditions provides insight into detection capability and eruption dynamics. Future work will implement AVO-G2S and high-resolution long-range infrasound propagation modeling in real-time for Alaskan volcanoes of interest.

Alaska

Gas geochemistry of the Mount Elbert Gas Hydrate Stratigraphic Test Well, Alaska North Slope: implications for gas hydrate exploration in the Arctic

Gases were analyzed from well cuttings, core, gas hydrate, and formation tests at the BPXA-DOE-USGS Mount Elbert Gas Hydrate Stratigraphic Test Well, drilled within the Milne Point Unit, Alaska North Slope. The well penetrated a portion of the Eileen gas hydrate deposit, which overlies the more deeply buried Prudhoe Bay, Milne Point, West Sak, and Kuparuk River oil fields. Gas sources in the upper 200 m are predominantly from microbial sources (C 1 isotopic compositions ranging from −86.4 to −80.6‰). The C 1 isotopic composition becomes progressively enriched from 200 m to the top of the gas hydrate-bearing sands at 600 m. The tested gas hydrates occur in two primary intervals, units D and C, between 614.0 m and 664.7 m, containing a total of 29.3 m of gas hydrate-bearing sands. The hydrocarbon gases in cuttings and core samples from 604 to 914 m are composed of methane with very little ethane. The isotopic composition of the methane carbon ranges from −50.1 to −43.9‰ with several outliers, generally decreasing with depth. Gas samples collected by the Modular Formation Dynamics Testing (MDT) tool in the hydrate-bearing units were similarly composed mainly of methane, with up to 284 ppm ethane. The methane isotopic composition ranged from −48.2 to −48.0‰ in the C sand and from −48.4 to −46.6‰ in the D sand. Methane hydrogen isotopic composition ranged from −238 to −230‰, with slightly more depleted values in the deeper C sand. These results are consistent with the concept that the Eileen gas hydrates contain a mixture of deep-sourced, microbially biodegraded thermogenic gas, with lesser amounts of thermogenic oil-associated gas, and coal gas. Thermal gases are likely sourced from existing oil and gas accumulations that have migrated up-dip and/or up-fault and formed gas hydrate in response to climate cooling with permafrost formation.

Alaska