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Hydrologic requirements of and consumptive ground-water use by riparian vegetation along the San Pedro River, Arizona

This study is a coordinated effort by the U.S. Geological Survey (USGS), the U.S. Department of Agriculture, Agricultural Research Service (USDA ARS), and Arizona State University, with assistance from the U.S. Army Corps of Engineers, the University of Wyoming, and the University of Arizona. The specific objectives of the study were: to determine the water needs of riparian vegetation through the riparian growing season and throughout the SPRNCA to ensure its long-term ecological integrity; to quantify the total water use of riparian vegetation within the SPRNCA; and to determine the source of water used by key riparian plant species within the SPRNCA. To meet these objectives, the study was divided into three elements: (1) a characterization of the status and variability of hydrologic factors within the riparian system (USGS), (2) a riparian biohydrology study to relate spatial and temporal aspects of riparian changes and condition to the hydrologic variables (Arizona State University), and (3) a water-use evapotranspiration (ET) study to quantify annual consumptive ground-water use by riparian transpiration and direct evaporation from the stream channel (USDA ARS) in cooperation with the U.S. Army Corps of Engineers, the University of Wyoming, and the University of Arizona. Twenty-six sites within the SPRNCA were selected for collection of vegetation data from three primary streamflow regimes (perennial, intermittent-wet, intermittent-dry), which include the principal vegetation communities. Detailed hydrologic-condition data were collected at a subset of 16 of these sites, called the SPRNCA biohydrology sites. Water-use and water-source data were collected at a subset of 5 of the 16 biohydrology sites. Vegetation data also were collected at supplemental sites within the SPRNCA boundary in the Upper San Pedro Basin and in the Lower San Pedro Basin. In addition to information about vegetation and geomorphic conditions, hydrologic data collected at the 16 biohydrology sites were used to delineate 14 reaches that were internally homogenous in terms of streamflow hydrology (spatial intermittence of streamflow) and geomorphology (channel sinuosity and flood-plain width). Although this overall study consisted of three elements, the elements were closely coordinated to derive integrated results. Specifically, the connection between water demand, water availability, and riparian functioning represents a synthesis of the study elements. The effects of intra- and inter-annual as well as spatial variability of hydrologic and riparian factors were observed in each of the three study elements.

Arizona

Microbial transformation of elements: The case of arsenic and selenium

Microbial activity is responsible for the transformation of at least one third of the elements in the periodic table. These transformations are the result of assimilatory, dissimilatory, or detoxification processes and form the cornerstones of many biogeochemical cycles. Arsenic and selenium are two elements whose roles in microbial ecology have only recently been recognized. Known as "essential toxins", they are required in trace amounts for growth and metabolism but are toxic at elevated concentrations. Arsenic is used as an osmolite in some marine organisms while selenium is required as selenocysteine (i.e. the twenty-first amino acid) or as a ligand to metal in some enzymes (e.g. FeNiSe hydrogenase). Arsenic resistance involves a small-molecular-weight arsenate reductase (ArsC). The use of arsenic and selenium oxyanions for energy is widespread in prokaryotes with representative organisms from the Crenarchaeota, thermophilic bacteria, low and high G+C gram-positive bacteria, and Proteobacteria. Recent studies have shown that both elements are actively cycled and play a significant role in carbon mineralization in certain environments. The occurrence of multiple mechanisms involving different enzymes for arsenic and selenium transformation indicates several different evolutionary pathways (e.g. convergence and lateral gene transfer) and underscores the environmental significance and selective impact in microbial evolution of these two elements.

International Microbiology

Hydrological information products for the Off-Project Water Program of the Klamath Basin Restoration Agreement

The Klamath Basin Restoration Agreement (KBRA) was developed by a diverse group of stakeholders, Federal and State resource management agencies, Tribal representatives, and interest groups to provide a comprehensive solution to ecological and water-supply issues in the Klamath Basin. The Off-Project Water Program (OPWP), one component of the KBRA, has as one of its purposes to permanently provide an additional 30,000 acre-feet of water per year on an average annual basis to Upper Klamath Lake through "voluntary retirement of water rights or water uses or other means as agreed to by the Klamath Tribes, to improve fisheries habitat and also provide for stability of irrigation water deliveries." The geographic area where the water rights could be retired encompasses approximately 1,900 square miles. The OPWP area is defined as including the Sprague River drainage, the Sycan River drainage downstream of Sycan Marsh, the Wood River drainage, and the Williamson River drainage from Kirk Reef at the southern end of Klamath Marsh downstream to the confluence with the Sprague River. Extensive, broad, flat, poorly drained uplands, valleys, and wetlands characterize much of the study area. Irrigation is almost entirely used for pasture. To assist parties involved with decisionmaking and implementation of the OPWP, the U.S. Geological Survey (USGS), in cooperation with the Klamath Tribes and other stakeholders, created five hydrological information products. These products include GIS digital maps and datasets containing spatial information on evapotranspiration, subirrigation indicators, water rights, subbasin streamflow statistics, and return-flow indicators. The evapotranspiration (ET) datasets were created under contract for this study by Evapotranspiration, Plus, LLC, of Twin Falls, Idaho. A high-resolution remote sensing technique known as Mapping Evapotranspiration at High Resolution and Internalized Calibration (METRIC) was used to create estimates of the spatial distribution of ET. The METRIC technique uses thermal infrared Landsat imagery to quantify actual evapotranspiration at a 30-meter resolution that can be related to individual irrigated fields. Because evaporation uses heat energy, ground surfaces with large ET rates are left cooler as a result of ET than ground surfaces that have less ET. As a consequence, irrigated fields appear in the Landsat images as cooler than nonirrigated fields. Products produced from this study include total seasonal and total monthly (April-October) actual evapotranspiration maps for 2004 (a dry year) and 2006 (a wet year). Maps showing indicators of natural subirrigation were also provided by this study. "Subirrigation" as used here is the evapotranspiration of shallow groundwater by plants with roots that penetrate to or near the water table. Subirrigation often occurs at locations where the water table is at or above the plant rooting depth. Natural consumptive use by plants diminishes the benefit of retiring water rights in subirrigated areas. Some agricultural production may be possible, however, on subirrigated lands for which water rights are retired. Because of the difficulty in precisely mapping and quantifying subirrigation, this study presents several sources of spatially mapped data that can be used as indicators of higher subirrigation probability. These include the floodplain boundaries defined by stream geomorphology, water-table depth defined in Natural Resources Conservation Service (NRCS) soil surveys, and soil rooting depth defined in NRCS soil surveys. The two water-rights mapping products created in the study were "points of diversion" (POD) and "place of use" (POU) for surface-water irrigation rights. To create these maps, all surface-water rights data, decrees, certificates, permits, and unadjudicated claims within the entire 1,900 square mile study area were aggregated into a common GIS geodatabase. Surface-water irrigation rights within a 5-mile buffer of the study area were then selected and identified. The POU area was then totaled by water right for primary and supplemental water rights. The maximum annual volume (acre-feet) allowed under each water right also was calculated using the POU area and duty (allowable annual irrigation application in feet). In cases where a water right has more than one designated POD, the total volume for the water right was equally distributed to each POD listed for the water right. Because of this, mapped distribution of diversion rates for some rights may differ from actual practice. Water-right information in the map products was from digital datasets obtained from the Oregon Water Resources Department and was, at the time acquired, the best available compilation of water-right information available. Because the completeness and accuracy of the water-right data could not be verified, users are encouraged to check directly with the Oregon Water Resources Department where specific information on individual rights or locations is essential. A dataset containing streamflow statistics for 72 subbasins in the study area was created for the study area. The statistics include annual flow durations (5-, 10-, 25-, 50-, and 95-percent exceedances) and 7-day, 10-year (7Q10) and 7-day, 2-year (7Q2) low flows, and were computed using regional regression equations based on measured streamflow records in the region. Daily streamflow records used were adjusted as needed for crop consumptive use; therefore the statistics represent streamflow under more natural conditions as though irrigation diversions did not exist. Statistics are provided for flow rates resulting from streamflow originating from within the entire drainage area upstream of the subbasin pour point (referring to the outlet of the contributing drainage basin). The statistics were computed for the purpose of providing decision makers with the ability to estimate streamflow that would be expected after water conservation techniques have been implemented or a water right has been retired. A final product from the study are datasets of indicators of the potential for subsurface return flow of irrigation water from agricultural areas to nearby streams. The datasets contain information on factors such as proximity to surface-water features, geomorphic floodplain characteristics, and depth to water. The digital data, metadata, and example illustrations for the datasets described in this report are available on-line from the USGS Water Resources National Spatial Data Infrastructure (NSDI) Node Website http://water.usgs.gov/lookup/getgislist or from the U.S. Government website DATA.gov at http://www.data.gov with links provided in a Microsoft® Excel® workbook in appendix A.

California;Oregon

Inter-comparison of hydro-climatic regimes across northern catchments: Synchronicity, resistance and resilience

The higher mid-latitudes of the Northern Hemisphere are particularly sensitive to climate change as small differences in temperature determine frozen ground status, precipitation phase, and the magnitude and timing of snow accumulation and melt. An international inter-catchment comparison program, North-Watch, seeks to improve our understanding of the sensitivity of northern catchments to climate change by examining their hydrological and biogeochemical responses. The catchments are located in Sweden (Krycklan), Scotland (Mharcaidh, Girnock and Strontian), the United States (Sleepers River, Hubbard Brook and HJ Andrews) and Canada (Catamaran, Dorset and Wolf Creek). This briefing presents the initial stage of the North-Watch program, which focuses on how these catchments collect, store and release water and identify 'types' of hydro-climatic catchment response. At most sites, a 10-year data of daily precipitation, discharge and temperature were compiled and evaporation and storage were calculated. Inter-annual and seasonal patterns of hydrological processes were assessed via normalized fluxes and standard flow metrics. At the annual-scale, relations between temperature, precipitation and discharge were compared, highlighting the role of seasonality, wetness and snow/frozen ground. The seasonal pattern and synchronicity of fluxes at the monthly scale provided insight into system memory and the role of storage. We identified types of catchments that rapidly translate precipitation into runoff and others that more readily store water for delayed release. Synchronicity and variance of rainfall-runoff patterns were characterized by the coefficient of variation (cv) of monthly fluxes and correlation coefficients. Principal component analysis (PCA) revealed clustering among like catchments in terms of functioning, largely controlled by two components that (i) reflect temperature and precipitation gradients and the correlation of monthly precipitation and discharge and (ii) the seasonality of precipitation and storage. By advancing the ecological concepts of resistance and resilience for catchment functioning, results provided a conceptual framework for understanding susceptibility to hydrological change across northern catchments. ?? 2010 John Wiley & Sons, Ltd.

Hydrological Processes

Ecological impacts of wheat seeding after a Sierra Nevada wildfire

The Highway Fire burned 1680 ha of mixed ponderosa pine–oak–chaparral in the newly created Giant Sequoia National Monument and the adjacent Sequoia National Forest of Fresno County, California in August 2001. The USDA Forest Service Burned Area Emergency Rehabilitation (BAER) program recommended that portions of the burned forest be seeded with a non-persistent variety of wheat at a density of 157 kg ha –1 (140 lb/ac). The present study compared the vascular plant diversity and cover in seeded and unseeded parts of this burn to evaluate the ecological impact of seeding an alien grass. In the first post-fire growing season, the natural regeneration of unseeded control sites averaged ~55% ground surface covered. Wheat seeding enhanced the ground cover, averaging 95% ground surface cover. Wheat was the dominant species on the seeded sites, comprising 67% of the total cover. Dominance–diversity curves were markedly affected by the seeding and indicated a disruption in the natural ecological structure of these communities. On seeded sites, wheat dominated and all other species were poorly represented whereas, on unseeded control sites, there was a more equitable distribution of species. Correlated with the wheat cover was a significant decrease in species richness at all scales examined. Total species richness was reduced from 152 species across all unseeded sites to 104 species on all seeded sites. Average species richness, at scales from 1 to 1000 m 2 , was 30–40% lower on seeded sites. Species most strongly inhibited were post-fire endemics whose lifecycle is restricted to immediate post-fire environments. Seeded sites had fewer alien species than unseeded sites; however, this may not have any lasting effect since other studies show the primary alien threat is not in the first post-fire year. Seeding was also associated with an order of magnitude drop in Pinus ponderosa seedling recruitment and, coupled with the massive thatch still remaining on the site, it is likely that recruitment will be inhibited in subsequent years.

International Journal of Wildland Fire

Counting at low concentrations: the statistical challenges of verifying ballast water discharge standards

Discharge from the ballast tanks of ships is one of the primary vectors of nonindigenous species in marine environments. To mitigate this environmental and economic threat, international, national, and state entities are establishing regulations to limit the concentration of living organisms that may be discharged from the ballast tanks of ships. The proposed discharge standards have ranged from zero detectable organisms to <10 organisms/m 3 . If standard sampling methods are used, verifying whether ballast discharge complies with these stringent standards will be challenging due to the inherent stochasticity of sampling. Furthermore, at low concentrations, very large volumes of water must be sampled to find enough organisms to accurately estimate concentration. Despite these challenges, adequate sampling protocols comprise a critical aspect of establishing standards because they help define the actual risk level associated with a standard. A standard that appears very stringent may be effectively lax if it is paired with an inadequate sampling protocol. We describe some of the statistical issues associated with sampling at low concentrations to help regulators understand the uncertainties of sampling as well as to inform the development of sampling protocols that ensure discharge standards are adequately implemented.

Ecological Applications

Target and suspect per- and polyfluoroalkyl substances in fish from an AFFF-impacted waterway

A major source of toxic per - and polyfluoroalkyl substances (PFAS) is aqueous film-forming foams (AFFF) used in firefighting and training at airports and military installations, however, PFAS have many additional sources in consumer products and industrial processes. A field study was conducted on fish tissues from three reaches of the Columbia Slough, located near Portland International Airport, OR, that are affected by AFFF and other PFAS sources. Fishes including largescale sucker ( Catostomus macrocheilus ), goldfish ( Carassius auratus ), and largemouth bass ( Micropterus salmoides ) were collected in 2019 and 2020. Fish blood, liver, and fillet (muscle) were analyzed for target and suspect PFAS by liquid chromatography high resolution mass spectrometry (LC-HRMS). Data were analyzed for patterns by fish species, tissue type, and river reach. Thirty-three out of 50 target PFAS and additional suspect compounds were detected at least once during the study, at concentrations up to 856 ng/g. Seven carboxylic acids (PFOA, PFNA, PFDA, PFUdA, PFDoA, PFTrDA, PFTeDA), three sulfonates (PFHxS, PFOS, PFDS), three electrofluorination-based compounds (FBSA, FHxSA, FOSA), and two fluorotelomer-based compounds (8:2 FTS, 10:2 FTS) were the most frequently detected compounds in all tissue types. The C6 (PFHxS) to C10 (PFDS) homologs were detected with PFOS and FHxSA at concentrations 1–3 orders of magnitude greater than the other PFAS detected. This is the first report of Cl-PFOS, FPeSA, and FHpSA detected in fish tissue. In all fish samples, fillet concentrations of PFAS were the lowest, followed by liver, and blood concentrations of PFAS were the highest. Differences in PFAS concentrations were driven primarily by tissue types and to a lesser extent fish species, but weakly by river reach. The Oregon Health Authority modified an existing fish consumption advisory on the Columbia Slough to recommend no whole-body consumption of most fish to avoid elevated levels of PFOS in fish liver. Measured PFAS concentrations in fish tissues indicate the potential for adverse ecological effects.

Oregon

Developing species-age cohorts from forest inventory and analysis data to parameterize a forest landscape model

Simulating long-term, landscape level changes in forest composition requires estimates of stand age to initialize succession models. Detailed stand ages are rarely available, and even general information on stand history often is lacking. We used data from USDA Forest Service Forest Inventory and Analysis (FIA) database to estimate broad age classes for a forested landscape to simulate changes in landscape composition and structure relative to climate change at Fort Drum, a 43,000 ha U.S. Army installation in northwestern New York. Using simple linear regression, we developed relationships between tree diameter and age for FIA site trees from the host and adjacent ecoregions and applied those relationships to forest stands at Fort Drum. We observed that approximately half of the variation in age was explained by diameter breast height (DBH) across all species studied ( r 2 = 0.42 for sugar maple Acer saccharum to 0.63 for white ash Fraxinus americana ). We then used age-diameter relationships from published research on northern hardwood species to calibrate results from the FIA-based analysis. With predicted stand age, we used tree species life histories and environmental conditions represented by ecological site types to parameterize a stochastic forest landscape model (LANDIS-II) to spatially and temporally model successional changes in forest communities at Fort Drum. Forest stands modeled over 100 years without significant disturbance appeared to reflect expected patterns of increasing dominance by shade-tolerant mesophytic tree species such as sugar maple, red maple ( Acer rubrum ), and eastern hemlock ( Tsuga canadensis ) where soil moisture was sufficient. On drier sandy soils, eastern white pine ( Pinus strobus ), red pine ( P. resinosa ), northern red oak ( Quercus rubra ), and white oak ( Q. alba ) continued to be important components throughout the modeling period with no net loss at the landscape scale. Our results suggest that despite abundant precipitation and relatively low evapotranspiration rates for the region, low soil water holding capacity and fertility may be limiting factors for the spread of mesophytic species on excessively drained soils in the region. Increasing atmospheric temperatures projected for the region could alter moisture regimes for many coarse-textured soils providing a possible mechanism for expansion of xerophytic tree species.

New York

Water-temperature data acquisition activities in the United States

Along with the growing interest in water quality during the last decade, the need for data on all types of water-quality parameters has also increased. One parameter of particular interest, because of its many ramifications, is temperature. It influences many of the chemical and physical processes that take place in water. The solubility of gases--for example, oxygen and carbon dioxide--and the solution of mineral matter in water are functions of temperature. Such physical properties as density and viscosity vary with temperature. Oxidation of organic materials, as well as algal and bacterial growth, is promoted or retarded by favorable or unfavorable temperatures. Further, temperature bears on the utility of water: as a source of public water supplies; for industrial use, particularly if the water is used for cooling; and in the field of recreation involving contact sports, fishing, and fish culture. In recent years, temperature changes resulting from inflow of heated industrial waste, particularly effluent from power generating plants, have increased the need for temperature data to determine the degree of change, its effect on ecology, and the effect of any remedial action. Thus, because of the many extensive and intensive effects, a large amount of temperature data is collected on surface and ground waters by many agencies throughout the country. Moreover, because of its importance, there is a widespread interest in temperature even by those who are not active collectors of the data themselves. The industrialist, the manager, the public official, and others at one time or another may have need for temperature data and may well raise the questions: Who is collecting temperature data? What is the extent of the activity? Where are the data being collected? The purpose of this report is to answer these questions. The information in the report is confined to the activities of Federal and non-Federal agencies. It is based on information furnished to the Office of Water Data Coordination, U.S. Geological Survey, and published in the "Catalog of Information on Water Data, Index to Water Quality Section, Edition 1970." This is one of four indexes, each of which is a separate section of the Catalog. Three of the indexes, "Index to Water-Quality Section," "Index to Surface-Water Section," and "Index to Ground-.Water Stations," contain information on data acquired on a recurrent basis at specific locations for a period of 3 years or more. The fourth section, "Index to Areal Investigations and Miscellaneous Activities," is concerned with specific projects or shorter-term data activities that involve field or laboratory measurements or observations not included in any other section of the Catalog. The Catalog is a record of activities throughout the country (and in some places along the international border between the United States and Canada) conducted by Federal and non-Federal agencies engaged in the acquisition of water data and who furnish such information for presentation in the Catalog. The Catalog itself is an outgrowth of an assignment to the Department of the Interior and in turn to the Geological Survey, by the Office of Management and Budget, through the medium of OMB Circular A-67. This Circular states in part that one of the assigned responsibilities will be maintenance of a "central catalog of information on...water data and on Federal activities being planned or conducted to acquire such data." As an extension of this activity, non-Federal agencies are solicited to participate in the program. In this report, information is presented by means of tables and illustrations preceded by brief explanations. It includes the agencies collecting the data, the number of stations located on surface and ground waters where temperature measurements are made, the distribution of stations by States and by the 21 regions of the Water Resources Council (WRC) (a Federal agency created in accordance with the Water Resources Planning Act of 1965), and the frequency of measurements. Acquisition activities were not reported for those areas left blank on some illustrations. The report does not contain the actual temperature data. For this information, the particular agency, Federal or non-Federal, acquiring the data will have to be contacted. However, a bibliography at the end of this report lists 194 references which contain temperature data. Many of these references, especially the publications of the Geological Survey, may be available in major public and university libraries and libraries of Federal and non-Federal agencies.

Water-Resources Investigations Report

Insect pollinator crossing of international border barriers along the U.S.-Mexico border

Infrastructure along the U.S.-Mexico Border may not be equally permeable to all types of insect pollinators with potential implications for pollen and gene flow between plant populations. Pollinators were observed on their approach to two types of border barriers (slatted and cemented) along the U.S.-Mexico Border from March 2023 to January 2024. Near the barrier, four insect behaviors were observed including 1) flying over the barrier, 2) crossing through the slats of the barrier, 3) not crossing the barrier, or 4) flying parallel to the barrier without crossing. Overall, 90.2% of the pollinators crossed the barrier. Butterflies were most often observed flying over the barrier (86.8%) or sometimes moving through the slats in the barrier (6.8%). It was more common for moths to crawl through the slats than to fly over the barrier based on the occurrence model. On windy days, both butterflies and moths sometimes flew parallel to the barrier without crossing (1.2% and 27.3%, respectively), although moth crossing behavior was not related to the abundance model. Butterfly abundance increased in higher temperatures and decreased in higher wind speeds. Other insect pollinators were also observed (bee, skipper, wasp) but their crossing behavior was not significantly related to the model. Because pollinators support endangered plant species, strategies to facilitate their barrier crossing could support plant conservation in South Texas.

Texas

Estimating suitable environments for invasive plant species across large landscapes: a remote sensing strategy using Landsat 7 ETM+

The key to reducing ecological and economic damage caused by invasive plant species is to locate and eradicate new invasions before they threaten native biodiversity and ecological processes. We used Landsat Enhanced Thematic Mapper Plus imagery to estimate suitable environments for four invasive plants in Big Bend National Park, southwest Texas, using a presence-only modeling approach. Giant reed ( Arundo donax ), Lehmann lovegrass ( Eragrostis lehmanniana ), horehound ( Marrubium vulgare ) and buffelgrass ( Pennisteum ciliare ) were selected for remote sensing spatial analyses. Multiple dates/seasons of imagery were used to account for habitat conditions within the study area and to capture phenological differences among targeted species and the surrounding landscape. Individual species models had high (0.91 to 0.99) discriminative ability to differentiate invasive plant suitable environments from random background locations. Average test area under the receiver operating characteristic curve (AUC) ranged from 0.91 to 0.99, indicating that plant predictive models exhibited high discriminative ability to differentiate suitable environments for invasive plant species from random locations. Omission rates ranged from <1.0 to 18%. We demonstrated that useful models estimating suitable environments for invasive plants may be created with <50 occurrence locations and that reliable modeling using presence-only datasets can be powerful tools for land managers.

Texas

Climate change projected to reduce prescribed burning opportunities in the south-eastern United States

Prescribed burning is a critical tool for managing wildfire risks and meeting ecological objectives, but its safe and effective application requires that specific meteorological criteria (a ‘burn window’) are met. Here, we evaluate the potential impacts of projected climatic change on prescribed burning in the south-eastern United States by applying a set of burn window criteria that capture temperature, relative humidity and wind speed to projections from an ensemble of Global Climate Models under two greenhouse gas emission scenarios. Regionally, the percentage of suitable days for burning changes little during winter but decreases substantially in summer owing to rising temperatures by the end of the 21st century compared with historical conditions. Management implications of such changes for six representative land management units include seasonal shifts in burning opportunities from summer to cool-season months, but with considerable regional variation. We contend that the practical constraints of rising temperatures on prescribed fire activities represent a significant future challenge and show that even meeting basic burn criteria (as defined today) will become increasingly difficult over time, which speaks to the need for adaptive management strategies to prepare for such changes.

Alabama, Arkansas, Florida, Georgia, kentucky, Lou

The utility of estimating net primary productivity over Alaska using baseline AVHRR data

Net primary productivity (NPP) is a fundamental ecological variable that provides information about the health and status of vegetation communities. The Normalized Difference Vegetation Index, or NDVI, derived from the Advanced Very High Resolution Radiometer (AVHRR) is increasingly being used to model or predict NPP, especially over large remote areas. In this article, seven seasonally based metrics calculated from a seven-year baseline NDVI dataset were used to model NPP over Alaska, USA. For each growing season, they included maximum, mean and summed NDVI, total days, product of total days and maximum NDVI, an integral estimate of NDVI and a summed product of NDVI and solar radiation. Field (plot) derived NPP estimates were assigned to 18 land cover classes from an Alaskan statewide land cover database. Linear relationships between NPP and each NDVI metric were analysed at four scales: plot, 1-km, 10-km and 20-km pixels. Results show moderate to poor relationship between any of the metrics and NPP estimates for all data sets and scales. Use of NDVI for estimating NPP may be possible, but caution is required due to data seasonality, the scaling process used and land surface heterogeneity.

Alaska

Molecular tracing of confiscated pangolin scales for conservation and illegal trade monitoring in Southeast Asia

Despite being protected by both international and national regulations, pangolins are threatened by illegal trade. Here we report mitochondrial DNA identification and haplotype richness estimation, using 239 pangolin scale samples from two confiscations in Hong Kong. We found a total of 13 genetically distinct cytochrome c oxidase I (COI) haplotypes in two confiscations (13 and ten haplotypes respectively, with ten shared haplotypes between confiscations). These haplotypes clustered in two distinct clades with one clade representing the Sunda pangolin (Manisjavanica). The other clade did not match with any known Asian pangolin sequences, and likely represented a cryptic pangolin lineage in Asia. By fitting sample coverage and rarefaction/regression models to our sample data, we predicted that the total number of COI haplotypes in two confiscations were 14.86 and 11.06 respectively, suggesting that our sampling caught the majority of haplotypes and that we had adequately characterized each confiscation. We detected substantial sequence divergence among the seized scales, likely evidencing that the Sunda pangolins were harvested over wide geographical areas across Southeast Asia. Our study illustrates the value of applying DNA forensics for illegal wildlife trade monitoring.

Global Ecology and Conservation

Anthropogenic influences on the input and biogeochemical cycling of nutrients and mercury in Great Salt Lake, Utah, USA

Despite the ecological and economic importance of Great Salt Lake (GSL), little is known about the input and biogeochemical cycling of nutrients and trace elements in the lake. In response to increasing public concern regarding anthropogenic inputs to the GSL ecosystem, the US Geological Survey (USGS) and US Fish and Wildlife Service (USFWS) initiated coordinated studies to quantify and evaluate the significance of nutrient and Hg inputs into GSL. A 6??? decrease in ??15N observed in brine shrimp (Artemia franciscana) samples collected from GSL during summer time periods is likely due to the consumption of cyanobacteria produced in freshwater bays entering the lake. Supporting data collected from the outflow of Farmington Bay indicates decreasing trends in ??15N in particulate organic matter (POM) during the mid-summer time period, reflective of increasing proportions of cyanobacteria in algae exported to GSL on a seasonal basis. The C:N molar ratio of POM in outflow from Farmington Bay decreases during the summer period, supportive of the increased activity of N fixation indicated by decreasing ??15N in brine shrimp and POM. Although N fixation is only taking place in the relatively freshwater inflows to GSL, data indicate that influx of fresh water influences large areas of the lake. Separation of GSL into two distinct hydrologic and geochemical systems from the construction of a railroad causeway in the late 1950s has created a persistent and widespread anoxic layer in the southern part of GSL. This anoxic layer, referred to as the deep brine layer (DBL), has high rates of SO42 - reduction, likely increasing the Hg methylation capacity. High concentrations of methyl mercury (CH3Hg) (median concentration = 24 ng/L) were observed in the DBL with a significant proportion (31-60%) of total Hg in the CH3Hg form. Hydroacoustic and sediment-trap evidence indicate that turbulence introduced by internal waves generated during sustained wind events can temporarily mix the elevated CH3Hg concentrations in the DBL with the more biologically active upper brine layer (UBL). Brine shrimp collected during the summer/fall time periods contained elevated Hg concentrations (median concentration = 0.34 mg/kg, dry weight (dw)) relative to samples collected during the spring (median concentration < 0.2 mg/kg, dw). Higher Hg in brine shrimp during the summer and fall may reflect the higher proportion of adult brine shrimp during this time period, resulting in an increased time for bioaccumulation of Hg. Eared grebes (Podiceps nigricollis) consume brine shrimp from GSL during the fall molting period. Median Hg concentrations in eared grebe livers increased by almost three times during the 3-5 month fall molting period. Selected duck species utilizing GSL have consistently exceeded the US Environmental Protection Agency (USEPA) screening level for Hg (0.3 mg/kg Hg wet weight), resulting in the issuance of warnings against unlimited human consumption of breast muscle tissue.

Applied Geochemistry

A global biodiversity observing system to unite monitoring and guide action

The rate and extent of global biodiversity change is surpassing our ability to measure, monitor and forecast trends. We propose an interconnected worldwide system of observation networks — a global biodiversity observing system (GBiOS) — to coordinate monitoring worldwide and inform action to reach international biodiversity targets.

Nature Ecology & Evolution

Estimating aboveground biomass in interior Alaska with Landsat data and field measurements

Terrestrial plant biomass is a key biophysical parameter required for understanding ecological systems in Alaska. An accurate estimation of biomass at a regional scale provides an important data input for ecological modeling in this region. In this study, we created an aboveground biomass (AGB) map at 30-m resolution for the Yukon Flats ecoregion of interior Alaska using Landsat data and field measurements. Tree, shrub, and herbaceous AGB data in both live and dead forms were collected in summers and autumns of 2009 and 2010. Using the Landsat-derived spectral variables and the field AGB data, we generated a regression model and applied this model to map AGB for the ecoregion. A 3-fold cross-validation indicated that the AGB estimates had a mean absolute error of 21.8 Mg/ha and a mean bias error of 5.2 Mg/ha. Additionally, we validated the mapping results using an airborne lidar dataset acquired for a portion of the ecoregion. We found a significant relationship between the lidar-derived canopy height and the Landsat-derived AGB (R 2 = 0.40). The AGB map showed that 90% of the ecoregion had AGB values ranging from 10 Mg/ha to 134 Mg/ha. Vegetation types and fires were the primary factors controlling the spatial AGB patterns in this ecoregion.

Alaska

Mapping fire regime ecoregions in California

The fire regime is a central framing concept in wildfire science and ecology and describes how a range of wildfire characteristics vary geographically over time. Understanding and mapping fire regimes is important for guiding appropriate management and risk reduction strategies and for informing research on drivers of global change and altered fire patterns. Most efforts to spatially delineate fire regimes have been conducted by identifying natural groupings of fire parameters based on available historical fire data. This can result in classes with similar fire characteristics but wide differences in ecosystem types. We took a different approach and defined fire regime ecoregions for California to better align with ecosystem types, without using fire as part of the definition. We used an unsupervised classification algorithm to segregate the state into spatial clusters based on distinctive biophysical and anthropogenic attributes that drive fire regimes – and then used historical fire data to evaluate the ecoregions. The fire regime ecoregion map corresponded well with the major land cover types of the state and provided clear separation of historical patterns in fire frequency and size, with lower variability in fire severity. This methodology could be used for mapping fire regimes in other regions with limited historical fire data or forecasting future fire regimes based on expected changes in biophysical characteristics.

California