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USGS2011

PRISM: Processing routines in IDL for spectroscopic measurements (installation manual and user's guide, version 1.0)

This report describes procedures for installing and using the U.S. Geological Survey Processing Routines in IDL for Spectroscopic Measurements (PRISM) software. PRISM provides a framework to conduct spectroscopic analysis of measurements made using laboratory, field, airborne, and space-based spectrometers. Using PRISM functions, the user can compare the spectra of materials of unknown composition with reference spectra of known materials. This spectroscopic analysis allows the composition of the material to be identified and characterized. Among its other functions, PRISM contains routines for the storage of spectra in database files, import/export of ENVI spectral libraries, importation of field spectra, correction of spectra to absolute reflectance, arithmetic operations on spectra, interactive continuum removal and comparison of spectral features, correction of imaging spectrometer data to ground-calibrated reflectance, and identification and mapping of materials using spectral feature-based analysis of reflectance data. This report provides step-by-step instructions for installing the PRISM software and running its functions.

Open-File Report↗

USGS1982

The Alaska Mineral Resource Assessment Program: Guide to information about the geology and mineral resources of the Ketchikan and Prince Rupert quadrangles, southeastern Alaska

The Ketchikan and Prince Rupert 1-degree by 2-degree quadrangles, which encompass about 16,000 km2 at the south tip of southeastern Alaska, have been investigated by integrated field and laboratory studies in the disciplines of geology, geochemistry, geophysics, and Landsat data interpretation to determine their mineral-resource potential. Mineral deposits in the study area have been mined or prospected intermittently since about 1900, and production of small tonnages of ores containing gold, silver, copper, lead, zinc, and tungsten has been recorded. Extensive exploration and development currently (1981) is underway at a molybdenum prospect about 65 km east of Ketchikan. Our mineral-resource assessment indicates that the area contains potentially significant amounts of those metallic commodities, as well as of molybdenum, iron, antimony, and barite. The results of these studies have been published in a folio of maps accompanied by descriptive texts, diagrams, tables, and pertinent references. The present report serves as a guide to these investigations, provides relevant background information, and integrates the component maps and reports. It also describes revisions to the geology based on studies completed since the folio was published and includes a list of specific and general references on the geology and mineral deposits of the study area.

Alaska↗

USGS2004

Combined interpretation of radar, hydraulic, and tracer data from a fractured-rock aquifer near Mirror Lake, New Hampshire, USA

An integrated interpretation of field experimental cross-hole radar, tracer, and hydraulic data demonstrates the value of combining time-lapse geophysical monitoring with conventional hydrologic measurements for improved characterization of a fractured-rock aquifer. Time-lapse difference-attenuation radar tomography was conducted during saline tracer experiments at the US Geological Survey Fractured Rock Hydrology Research Site near Mirror Lake, Grafton County, New Hampshire, USA. The presence of electrically conductive saline tracer effectively illuminates permeable fractures or pathways for geophysical imaging. The geophysical results guide the construction of three-dimensional numerical models of ground-water flow and solute transport. In an effort to explore alternative explanations for the tracer and tomographic data, a suite of conceptual models involving heterogeneous hydraulic conductivity fields and rate-limited mass transfer are considered. Calibration data include tracer concentrations, the arrival time of peak concentration at the outlet, and steady-state hydraulic head. Results from the coupled inversion procedure suggest that much of the tracer mass migrated outside the three tomographic image planes, and that solute is likely transported by two pathways through the system. This work provides basic and site-specific insights into the control of permeability heterogeneity on ground-water flow and solute transport in fractured rock.

New Hampshire↗

USGS1993

Geologic map of upper Eocene to Holocene volcanic and related rocks in the Cascade Range, Washington

Since 1979 the Geothermal Research Program of the U.S. Geological Survey has carried out a multidisciplinary research effort in the Cascade Range. The goal of this research is to understand the geology, tectonics, and hydrology of the Cascades in order to characterize and quantify geothermal resource potential. A major goal of the program is compilation of a comprehensive geologic map of the entire Cascade Range that incorporates modern field studies and that has a unified and internally consistent explanation. This map is one of a series presently being compiled that shows Cascade Range geology by fitting published and unpublished mapping into a province-wide scheme of lithostratigraphic units; map sheets of the Cascade Range in California and in Oregon complete the series. The complete series forms a guide to exploration and evaluation of the geothermal resources of the Cascade Range and will be useful for studies of volcanic hazards, volcanology, and tectonics. For geothermal reasons, the maps emphasize Quaternary volcanic rocks. Large igneous-related geothermal systems that have high temperatures are associated with Quaternary volcanic fields, and geothermal potential declines rapidly as age increases (Smith and Shaw, 1975). Most high-grade recoverable geothermal energy is likely to be associated with silicic volcanism less than 1 Ma. Lower grade (= lower temperature) geothermal resources may be associated with somewhat older rocks; however, volcanic rocks older than about 2 Ma are unlikely geothermal targets (Smith and Shaw, 1975).

Washington↗

USGS2020

Inferring surface flow velocities in sediment-laden Alaskan rivers from optical image sequences acquired from a helicopter

The remote, inaccessible location of many rivers in Alaska creates a compelling need for remote sensing approaches to streamflow monitoring. Motivated by this objective, we evaluated the potential to infer flow velocities from optical image sequences acquired from a helicopter deployed above two large, sediment-laden rivers. Rather than artificial seeding, we used an ensemble correlation particle image velocimetry (PIV) algorithm to track the movement of boil vortices that upwell suspended sediment and produce a visible contrast at the water surface. This study introduced a general, modular workflow for image preparation (stabilization and geo-referencing), preprocessing (filtering and contrast enhancement), analysis (PIV), and postprocessing (scaling PIV output and assessing accuracy via comparison to field measurements). Applying this method to images acquired with a digital mapping camera and an inexpensive video camera highlighted the importance of image enhancement and the need to resample the data to an appropriate, coarser pixel size and a lower frame rate. We also developed a Parameter Optimization for PIV (POP) framework to guide selection of the interrogation area (IA) and frame rate for a particular application. POP results indicated that the performance of the PIV algorithm was highly robust and that relatively large IAs (64–320 pixels) and modest frame rates (0.5–2 Hz) yielded strong agreement ( R 2 > 0 . 9 "> R 2 > 0 . 9 ) between remotely sensed velocities and field measurements. Similarly, analysis of the sensitivity of PIV accuracy to image sequence duration showed that dwell times as short as 16 s would be sufficient at a frame rate of 1 Hz and could be cut in half if the frame rate were doubled. The results of this investigation indicate that helicopter-based remote sensing of velocities in sediment-laden rivers could contribute to noncontact streamgaging programs and enable reach-scale mapping of flow fields.

Alaska↗

USGS2012

Telemetry techniques: A user guide for fisheries research

Telemetry provides a powerful and flexible tool for studying fish and other aquatic animals, and its use has become increasingly commonplace. However, telemetry is gear intensive and typically requires more specialized knowledge and training than many other field techniques. As with other scientific methods, collecting good data is dependent on an understanding of the underlying principles behind the approach, knowing how to use the equipment and techniques properly, and recognizing what to do with the data collected. This book provides a road map for using telemetry to study aquatic animals, and provides the basic information needed to plan, implement, and conduct a telemetry study under field conditions. Topics include acoustic or radio telemetry study design, tag implantation techniques, radio and acoustic telemetry principles and case studies, and data management and analysis. Chapters are written by biologists, technicians, and engineers from the private, academic, and government sectors, with decades of experience using these technologies.

Book↗

USGS2007

Spectral element modelling of fault-plane reflections arising from fluid pressure distributions

The presence of fault-plane reflections in seismic images, besides indicating the locations of faults, offers a possible source of information on the properties of these poorly understood zones. To better understand the physical mechanism giving rise to fault-plane reflections in compacting sedimentary basins, we numerically model the full elastic wavefield via the spectral element method (SEM) for several different fault models. Using well log data from the South Eugene Island field, offshore Louisiana, we derive empirical relationships between the elastic parameters (e.g. P-wave velocity and density) and the effective-stress along both normal compaction and unloading paths. These empirical relationships guide the numerical modelling and allow the investigation of how differences in fluid pressure modify the elastic wavefield. We choose to simulate the elastic wave equation via SEM since irregular model geometries can be accommodated and slip boundary conditions at an interface, such as a fault or fracture, are implemented naturally. The method we employ for including a slip interface retains the desirable qualities of SEM in that it is explicit in time and, therefore, does not require the inversion of a large matrix. We performa complete numerical study by forward modelling seismic shot gathers over a faulted earth model using SEM followed by seismic processing of the simulated data. With this procedure, we construct post-stack time-migrated images of the kind that are routinely interpreted in the seismic exploration industry. We dip filter the seismic images to highlight the fault-plane reflections prior to making amplitude maps along the fault plane. With these amplitude maps, we compare the reflectivity from the different fault models to diagnose which physical mechanism contributes most to observed fault reflectivity. To lend physical meaning to the properties of a locally weak fault zone characterized as a slip interface, we propose an equivalent-layer model under the assumption of weak scattering. This allows us to use the empirical relationships between density, velocity and effective stress from the South Eugene Island field to relate a slip interface to an amount of excess pore-pressure in a fault zone. ?? 2007 The Authors Journal compilation ?? 2007 RAS.

Geophysical Journal International↗

USGS2013

Monitoring sea lamprey pheromones and their degradation using rapid stream-side extraction coupled with UPLC-MS/MS

Pheromones guide adult sea lamprey (Petromyzon marinus) to suitable spawning streams and mates, and therefore, when quantified, can be used to assess population size and guide management. Here, we present an efficient sample preparation method where 100 mL of river water was spiked with deuterated pheromone as an internal standard and underwent rapid field-based SPE and elution in the field. The combination of field extraction with laboratory UPLC-MS/MS reduced the sample consumption from 1 to 0.1 L, decreased the sample process time from more than 1 h to 10 min, and increased the precision and accuracy. The sensitivity was improved more than one order of magnitude compared with the previous method. The influences of experimental conditions were assessed to optimize the separation and peak shapes. The analytical method has been validated by studies of stability, selectivity, precision, and linearity and by the determination of the limits of detection and quantification. The method was used to quantify pheromone concentration from five streams tributary to Lake Ontario and to estimate that the environmental half-life of 3kPZS is about 26 h.

Journal of Separation Science↗

USGS2024

Bird community response to one decade of riparian restoration along the Colorado River delta in Mexico

We assessed the response of breeding birds to one decade of riparian restoration in the Colorado River delta including active vegetation management since 2010 and various environmental water deliveries since 2014. Bird surveys were conducted from 2002 to 2021 at 230 bird count stations distributed along five river reaches with different hydrogeomorphic characteristics, across 7 routes in actively revegetated (“restored”) sites, and 20 routes in non-actively revegetated (“control”) sites. Both restored and control sites could be affected by environmental flows/water deliveries. We examined the temporal trajectories of four community-aggregated metrics for 53 breeding species, and the number of detections (counts) for the 30 most frequently detected among the 53 species at the restored and control sites, using generalized linear mixed-effects models. Diversity and richness of the 53 breeding species were highest in the wettest river reach, but higher than in control sites in only one restored site within one of the driest river reaches. The avian community was recovering in control sites, ameliorating a long-term declining trend, perhaps indicating indirect positive effects of active restoration. The response to restoration of the 30 most common species was species-specific. Ten of the 16 riparian forest specialist species had more detections in restored sites of the wettest reach (vs. only two in the driest reach). Species with preference for agricultural fields and generalists had the highest counts along the entire delta. Generalists generally decreased following revegetation, which was another positive restoration effect. Our results will be used to help guide future restoration efforts.

Ecological Engineering↗

USGS2021

Small atoll fresh groundwater lenses respond to a combination of natural climatic cycles and human modified geology

Freshwater lenses underlying small ocean islands exhibit spatial variability and temporal fluctuations in volume, influencing ecologic management. For example, The Palmyra Atoll National Wildlife Refuge harbors one of the few surviving native stands of Pisonia grandis in the central Pacific Ocean, yet these trees face pressure from groundwater salinization, with little basic groundwater data to guide decision making. Adding to natural complexity, the geology of Palmyra was heavily altered by dredge and fill activities. Our study based at this atoll combines geophysical and hydrological field measurements from 2008 to 2019 with groundwater modeling to study the drivers of observed freshwater lens dynamics. Electromagnetic induction (EMI) field data were collected on the main atoll islands over repeat transects in 2008 following ‘strong’ La Niña conditions (wet) and in 2016 during ‘very strong’ El Niño conditions (dry). Shallow monitoring wells were installed adjacent to the geophysical transects in 2013 and screened within the fresh/saline groundwater transition zone. Temporal EMI and monitoring well data showed a strong contraction of the freshwater lens in response to El Niño conditions, and indicated a thicker lens toward the ocean side, an opposite spatial pattern to that observed for many other Pacific islands. On an outer islet where a stand of mature Pisonia trees exist, EMI surveys revealed only a thin (<3 m from land surface) layer of brackish groundwater during El Niño. Numerical groundwater simulations were performed for a range of permeability distributions and climate conditions at Palmyra. Results revealed that the observed atypical lens asymmetry is likely due to more efficient submarine groundwater discharge on the lagoon side as a result of lagoon dredging and filling with high-permeability material. Simulations also predict large decreases (40%) in freshwater lens volume during dry cycles and highlight threats to the Pisonia trees, yielding insight for atoll ecosystem management worldwide.

Science of the Total Environment↗

USGS2014

The Tetracorder user guide: version 4.4

Imaging spectroscopy mapping software assists in the identification and mapping of materials based on their chemical properties as expressed in spectral measurements of a planet including the solid or liquid surface or atmosphere. Such software can be used to analyze field, aircraft, or spacecraft data; remote sensing datasets; or laboratory spectra. Tetracorder is a set of software algorithms commanded through an expert system to identify materials based on their spectra (Clark and others, 2003). Tetracorder also can be used in traditional remote sensing analyses, because some of the algorithms are a version of a matched filter. Thus, depending on the instructions fed to the Tetracorder system, results can range from simple matched filter output, to spectral feature fitting, to full identification of surface materials (within the limits of the spectral signatures of materials over the spectral range and resolution of the imaging spectroscopy data). A basic understanding of spectroscopy by the user is required for developing an optimum mapping strategy and assessing the results.

Open-File Report↗

USGS2022

Simplifying complex fault data for systems-level analysis: Earthquake geology inputs for U.S. NSHM 2023

As part of the U.S. National Seismic Hazard Model (NSHM) update planned for 2023, two databases were prepared to more completely represent Quaternary-active faulting across the western United States: the NSHM23 fault sections database (FSD) and earthquake geology database (EQGeoDB). In prior iterations of NSHM, fault sections were included only if a field-measurement-derived slip rate was estimated along a given fault. By expanding this inclusion criteria, we were able to assess a larger set of faults for use in NSHM23. The USGS Quaternary Fault and Fold Database served as a guide for assessing possible additions to the NSHM23 FSD. Reevaluating available data from published sources yielded an increase of fault sections from ~650 faults in NSHM18 to ~1,000 faults proposed for use in NSHM23. EQGeoDB, a companion dataset linked to NSHM23 FSD, contains geologic slip rate estimates for fault sections included in FSD. Together, these databases serve as common input data used in deformation modeling, earthquake rupture forecasting, and additional downstream uses in NSHM development.

Scientific Data↗

USGS2004

Preliminary geologic map of the Big Bear City 7.5' Quadrangle, San Bernardino County, California

This data set maps and describes the geology of the Big Bear City 7.5' quadrangle, San Bernardino County, California. Created using Environmental Systems Research Institute's ARC/INFO software, the data base consists of the following items: (1) a rock-unit coverage and attribute tables (polygon and arc) containing geologic contacts, units and rock-unit labels as annotation which are also included in a separate annotation coverage, bbc_anno (2) a point coverage containing structural point data and (3) a coverage containing fold axes. In addition, the data set includes the following graphic and text products: (1) A PostScript graphic plot-file containing the geologic map, topography, cultural data, a Correlation of Map Units (CMU) diagram, a Description of Map Units (DMU), an index map, a regional geologic and structure map, and an explanation for point and line symbols; (2) PDF files of the Readme (including the metadata file as an appendix), and a screen graphic of the plot produced by the PostScript plot file. The geologic map describes a geologically complex area on the north side of the San Bernardino Mountains. Bedrock units in the Big Bear City quadrangle are dominated by (1) large Cretaceous granitic bodies ranging in composition from monzogranite to gabbro, (2) metamorphosed sedimentary rocks ranging in age from late Paleozoic to late Proterozoic, and (3) Middle Proterozoic gneiss. These rocks are complexly deformed by normal, reverse, and thrust faults, and in places are tightly folded. The geologic map database contains original U.S. Geological Survey data generated by detailed field observation and by interpretation of aerial photographs. The map data was compiled on base-stable cronoflex copies of the Big Bear City 7.5' topographic map, transferred to a scribe-guide and subsequently digitized. Lines, points, and polygons were edited at the USGS using standard ARC/INFO commands. Digitizing and editing artifacts significant enough to display at a scale of 1:24,000 were corrected. Within the database, geologic contacts are represented as lines (arcs), geologic units as polygons, and site-specific data as points. Polygon, arc, and point attribute tables (.pat, .aat, and .pat, respectively) uniquely identify each geologic datum.

Open-File Report↗

USGS1980

Digital terrain tapes: user guide

DMATC's digital terrain tapes are a by-product of the agency's efforts to streamline the production of raised-relief maps. In the early 1960's DMATC developed the Digital Graphics Recorder (DGR) system that introduced new digitizing techniques and processing methods into the field of three-dimensional mapping. The DGR system consisted of an automatic digitizing table and a computer system that recorded a grid of terrain elevations from traces of the contour lines on standard topographic maps. A sequence of computer accuracy checks was performed and then the elevations of grid points not intersected by contour lines were interpolated. The DGR system produced computer magnetic tapes which controlled the carving of plaster forms used to mold raised-relief maps. It was realized almost immediately that this relatively simple tool for carving plaster molds had enormous potential for storing, manipulating, and selectively displaying (either graphically or numerically) a vast number of terrain elevations. As the demand for the digital terrain tapes increased, DMATC began developing increasingly advanced digitizing systems and now operates the Digital Topographic Data Collection System (DTDCS). With DTDCS, two types of data elevations as contour lines and points, and stream and ridge lines are sorted, matched, and resorted to obtain a grid of elevation values for every 0.01 inch on each map (approximately 200 feet on the ground). Undefined points on the grid are found by either linear or or planar interpolation.

Report↗

USGS2023

User’s Guide to planetary image analysis and geologic mapping in ArcGIS Pro

Geologic maps are valuable tools in planetary science. Though planetary geologic maps are similar to terrestrial (Earthbased) geologic maps, the nature of planetary exploration introduces unique challenges for geologic mappers. Terrestrial geologic mappers prepare products from field-based observation, often comparing or refining those with aerial and (or) orbital images. Planetary geologic mapping relies almost exclusively on remote observations, which are made by orbiting spacecraft. Therefore, with a few exceptions for locations with rovers, landers, or crewed surface missions, planetary geologic mappers are not able to observe their map area in detail or at smaller scales. As a result, they must interpret and describe their map features differently than those used in terrestrial geologic maps. For example, terrestrial geologic mappers commonly divide units by lithology (rock type) or grain size. However, planetary geologic mappers often do not have detailed enough information to know what type of rock or grain size is present, and instead must divide the planet’s surface into geologic units using differences in tone, color, and surface texture (at multiple scales). Cross-cutting relationships, where apparent, can provide excellent—and often crucial—observations for identifying and subdividing geologic units using orbital datasets. The process of creating planetary maps has evolved over time, from original hand-drawn maps created during the early 1800s through the late 1900s, to the fully digital products created today. Modern-day planetary geologic mapping uses Geographic Information Systems (GIS) software and tools to visualize data, delineate units and landforms, and accurately convey spatial relationships to a map user using cartographic features represented by points, lines, and polygons. This tutorial was written to familiarize both new and experienced planetary geologic mappers with ArcGIS Pro, a commonly used GIS software package developed by Esri. This tutorial introduces new planetary geologic mappers to fundamental concepts and best practices in planetary geologic mapping. For mappers with experience using ArcMap (a previous version of ArcGIS), this tutorial will help to familiarize users with new changes in layout and functionality, so current projects may be migrated into the ArcGIS Pro environment. No prior knowledge is required, although a general familiarity with geology, GIS, and planetary science is recommended. This tutorial includes links to helpful glossaries of common GIS terms and other GIS and planetary science resources in appendix 1. For additional information on planetary geologic mapping, see the U.S Geological Survey (USGS) Astrogeology NASA Planetary Geologic Mapping Program website and the Planetary Mapping Guidelines. Numerous ArcGIS tutorials are also available from Esri’s Tutorials page, through the Esri Academy catalog, and Esri’s ArcGIS Pro Resources page.

Techniques and Methods↗

USGS2015

Assessing juvenile salmon rearing habitat and associated predation risk in a lower Snake River reservoir

Subyearling fall Chinook salmon (Oncorhynchus tshawytscha) in the Columbia River basin exhibit a transient rearing strategy and depend on connected shoreline habitats during freshwater rearing. Impoundment has greatly reduced the amount of shallow-water rearing habitat that is exacerbated by the steep topography of reservoirs. Periodic dredging creates opportunities to strategically place spoils to increase the amount of shallow-water habitat for subyearlings while at the same time reducing the amount of unsuitable area that is often preferred by predators. We assessed the amount and spatial arrangement of subyearling rearing habitat in Lower Granite Reservoir on the Snake River to guide future habitat improvement efforts. A spatially explicit habitat assessment was conducted using physical habitat data, two-dimensional hydrodynamic modelling and a statistical habitat model in a geographic information system framework. We used field collections of subyearlings and a common predator [smallmouth bass (Micropterus dolomieu)] to draw inferences about predation risk within specific habitat types. Most of the high-probability rearing habitat was located in the upper half of the reservoir where gently sloping landforms created low lateral bed slopes and shallow-water habitats. Only 29% of shorelines were predicted to be suitable (probability >0.5) for subyearlings, and the occurrence of these shorelines decreased in a downstream direction. The remaining, less suitable areas were composed of low-probability habitats in unmodified (25%) and riprapped shorelines (46%). As expected, most subyearlings were found in high-probability habitat, while most smallmouth bass were found in low-probability locations. However, some subyearlings were found in low-probability habitats, such as riprap, where predation risk could be high. Given their transient rearing strategy and dependence on shoreline habitats, subyearlings could benefit from habitat creation efforts in the lower reservoir where high-probability habitat is generally lacking. Published 2015. This article is a U.S. Government work and is in the public domain in the USA.

Lower Snake river reservior↗

USGS2014

Quaternary geology of the Boston area: Glacial events from Lake Charles to Lake Aberjona

The multiple-glacial and glaciomarine Quaternary history of the Boston, Massachusetts area has been known generally since the earliest studies of the then newly recognized glacial deposits described by Prof. Louis Agassiz in the late1840’s and fossil marine shells in the drift in the 1850’s. Attention then turned to possible glacial erosional effects on the preglacial bedrock physiography, as related to rock units and structure, and to the challenges of defining useful physical and lithic characteristics of the drift by Prof. W.O. Crosby and others, 1880-1900. The problems of deducing the relative stratigraphic order among such small, fossil-barren surficial sedimentary deposits, and extending knowledge gained from studies of postulated ancient glacial lakes to a regional understanding of the history of many lakes during the retreat of the ice sheet required field work and use of geologic maps. With the advent of modern topographic maps in the 1880’s, the early period of discovery included field studies of glacial lake deposits in local river basins in the Boston region, basins that drain northward, thereby creating glacial lake basins dammed by the ice margin as it retreated to the north. Guided by M.I.T. and Harvard professors W.O. Crosby, N.S. Shaler, J.B. Woodworth, W.M. Davis, and others in the 1880-1920 period, the first Quaternary glacial stratigraphers were students (e.g. Crosby and Grabau, 1896, Clapp, 1905, Fuller 1905, Goldthwaite 1906, Grabau, 1906, Taylor, Tight).

Massachusetts, New Hampshire, Rhode Island↗

USGS2024

A guide to environmental DNA extractions for non-molecular trained biologists, ecologists, and conservation scientists

Ecologists, biologists, and conservation scientists are increasingly interested in the use of environmental DNA (eDNA) data for research and potentially decision-making. While commercial DNA extraction kits are typically user-friendly and accessible, they may fail to deliver the desired results with inherently complex eDNA samples, necessitating protocol optimization or educated selection of alternative approaches. To this end, knowledge of the basic steps and principles of DNA extractions is essential, but traditional education tracks in ecology, conservation, and environmental management typically do not include in-depth training in molecular methods. The primary objective of this paper is to enable scientists with an ecological background and limited molecular training to understand the four key steps of eDNA isolations, and to use this expertise to their advantage. We describe the purpose of commonly used reagents and chemicals, point out alternatives for each key step, explain the impact of certain choices regarding isolation approaches on DNA integrity and purity, and highlight the possibility of a tailor-made “mix and match” approach. We anticipate that this paper will enable field ecologists to develop a deeper understanding of the mechanisms and chemistry underlying eDNA extractions, thus allowing them to make informed decisions regarding the best eDNA extraction method for their research goals. Our intention is not to provide comprehensive, step-by-step protocols, but to offer guiding principles while highlighting alternative solutions. Finally, we hope that this paper will act as a useful resource to support knowledge transfer and teaching.

Environmental DNA↗