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Groundwater quality in the Columbia Plateau, Snake River Plain, and Oahu basaltic-rock and basin-fill aquifers in the Northwestern United States and Hawaii, 1992-2010

This assessment of groundwater-quality conditions of the Columbia Plateau, Snake River Plain, and Oahu for the period 1992–2010 is part of the U.S. Geological Survey’s National Water Quality Assessment (NAWQA) program. It shows where, when, why, and how specific water-quality conditions occur in groundwater of the three study areas and yields science-based implications for assessing and managing the quality of these water resources. The primary aquifers in the Columbia Plateau, Snake River Plain, and Oahu are mostly composed of fractured basalt, which makes their hydrology and geochemistry similar. In spite of the hydrogeologic similarities, there are climatic differences that affect the agricultural practices overlying the aquifers, which in turn affect the groundwater quality. Understanding groundwater-quality conditions and the natural and human factors that control groundwater quality is important because of the implications to human health, the sustainability of rural agricultural economies, and the substantial costs associated with land and water management, conservation, and regulation. The principal regional aquifers of the Columbia Plateau, Snake River Plain, and Oahu are highly vulnerable to contamination by chemicals applied at the land surface; essentially, they are as vulnerable as many shallow surficial aquifers elsewhere. The permeable and largely unconfined character of principal aquifers in the Columbia Plateau, Snake River Plain, and Oahu allow water and chemicals to infiltrate to the water table despite depths to water commonly in the hundreds of feet. The aquifers are essentially unconfined over large areas, having few extensive clay layers to impede infiltration through permeable volcanic rock and alluvial sediments. Agriculture is intensive in all three study areas, and heavy irrigation has imposed large artificial flows of irrigation recharge that rival or exceed natural recharge rates. Fertilizers and pesticides applied at land surface are leached from soil and transported to deep water tables with the infiltrating irrigation recharge, resulting in a layer of degraded water quality overlying better quality regional groundwater beneath. This “irrigation-recharge layer” is best known on Oahu, where it has been studied since the 1960s; however, the extent of nitrate and pesticide contamination in the Columbia Plateau and Snake River Plain indicate that the same situation exists in those areas. Contamination from agricultural and urban activities is present not only at shallow depths in surficial materials of the three areas, but extends regionally in the deep, principal bedrock aquifers that are tapped for drinking water by domestic and public-supply wells. Naturally occurring constituents and nitrate concentrations above human-health benchmarks—Maximum Contaminant Levels (MCLs), and Health-Based Screening Levels (HBSLs)—were more common in the Columbia Plateau and the Snake River Plain than in Oahu. Concentrations of anthropogenic constituents (constituents related to human activities) above human-health benchmarks were more common in Oahu. Naturally occurring contaminants, such as arsenic and radon, may be present in groundwater at concentrations of potential concern for human health in relatively undeveloped settings that otherwise may not be perceived as susceptible to contamination. Even though the median depth to groundwater in Oahu is more than 300 feet, the common occurrence of anthropogenic compounds in groundwater indicates that Oahu has a high susceptibility to contamination. Nitrate concentrations in groundwater were above the national background concentrations of 1 milligram per liter (mg/L) in all three study areas. In the Columbia Plateau, nitrate exceeded the human-health benchmark of 10 mg/L in 20 percent of the wells sampled. In the Snake River Plain, nitrate exceeded the human-health benchmark of 10 mg/L in 3 percent of the wells sampled. Nitrate can persist in groundwater for years and even decades in the oxygen-rich groundwater of the Columbia Plateau and the Snake River Plain, so prudent groundwater protection measures are critical to protect drinking water resources by reducing nitrate leaching from the land surface. Nitrate logistic regression models indicated that areas with a high percentage of land in crops (such as potatoes or sugarcane) and soils with low amounts of organic matter are most likely to have elevated nitrate concentrations in the groundwater. Areas where agricultural activities were absent had much lower probabilities of detecting elevated nitrate concentrations. The Columbia Plateau had a much higher probability of having elevated nitrate concentrations, with most of the land area having greater than a 50 percent probability of elevated nitrate concentrations. Oahu and the Snake River Plain had a much lower probability of having elevated nitrate concentrations because of their lower percentage of agricultural land. Pesticides were detected at many sites in groundwater of the Columbia Plateau, Snake River Plain, and Oahu but generally at low concentrations below human-health benchmarks. Atrazine and its degradate (a compound produced from the breakdown of a parent pesticide), deethylatrazine, were the most commonly detected pesticides in groundwater sampled in the Columbia Plateau and Snake River Plain. Bromacil was the most commonly detected pesticide on Oahu. The other pesticides most commonly detected in the study areas include simazine, hexazinone, metribuzin, diuron, prometon, metolachlor, p,p’ -DDE, dieldrin, 2-4-D, and alachlor. DDE (a degradate of DDT) and dieldrin are still being detected in groundwater despite having been banned for more than 30 years. Codetection of multiple pesticides in water from a single well was common. The widespread occurrence of pesticides in groundwater in the study areas indicates that the groundwater is highly susceptible to pesticide contamination. Some pesticides were detected in groundwater samples from all three study areas, but other pesticides were detected only in samples from Oahu, or only in samples from the Columbia Plateau and Snake River Plain. This is because some pesticides (such as atrazine) are broad-spectrum pesticides that are used on many crops in many different areas of the United States. Other pesticides (such as simazine, metribuzin, and metolachlor) are used on row crops (such as potatoes, barley, and alfalfa) grown in the Columbia Plateau and Snake River Plain, but not on pineapple or sugarcane grown in Oahu. Atrazine logistic-regression models indicate that areas with a high percentage of land in crops (such as potatoes or sugarcane), a low percentage of fallow land, and highly permeable soils with low amounts of organic matter are most likely to have atrazine detected in the groundwater. Areas where agricultural activities were absent had much lower probabilities of atrazine being detected. The Snake River Plain had a much higher probability of atrazine detections, with more than 50 percent of the land area having greater than a 50 percent probability of atrazine contamination. Oahu had a much lower probability of atrazine contamination, with only 24 percent of the land area having greater than a 50 percent probability of atrazine contamination. Oahu and the Columbia Plateau had some of the highest percentages of soil fumigant detections in groundwater in the United States. Soil fumigants are volatile organic compounds (VOCs) used as pesticides, which are applied to soils to reduce populations of plant parasitic nematodes (harmful rootworms), weeds, fungal pathogens, and other soil-borne microorganisms. They are used in Oahu and the Columbia Plateau on crops such as pineapple and potatoes. All three areas (Columbia Plateau, Snake River Plain, and Oahu) had fumigant concentrations exceeding human-health benchmarks for drinking water.

Hawai'i, Idaho, Nevada, Oregon, Utah, Washington,

Water-quality conditions near the confluence of the Snake and Boise Rivers, Canyon County, Idaho

Total Maximum Daily Loads (TMDLs) have been established under authority of the Federal Clean Water Act for the Snake River-Hells Canyon reach, on the border of Idaho and Oregon, to improve water quality and preserve beneficial uses such as public consumption, recreation, and aquatic habitat. The TMDL sets targets for seasonal average and annual maximum concentrations of chlorophyll- a at 14 and 30 micrograms per liter, respectively. To attain these conditions, the maximum total phosphorus concentration at the mouth of the Boise River in Idaho, a tributary to the Snake River, has been set at 0.07 milligrams per liter. However, interactions among chlorophyll- a , nutrients, and other key water-quality parameters that may affect beneficial uses in the Snake and Boise Rivers are unknown. In addition, contributions of nutrients and chlorophyll- a loads from the Boise River to the Snake River have not been fully characterized. To evaluate seasonal trends and relations among nutrients and other water-quality parameters in the Boise and Snake Rivers, a comprehensive monitoring program was conducted near their confluence in water years (WY) 2009 and 2010. The study also provided information on the relative contribution of nutrient and sediment loads from the Boise River to the Snake River, which has an effect on water-quality conditions in downstream reservoirs. State and site-specific water-quality standards, in addition to those that relate to the Snake River-Hells Canyon TMDL, have been established to protect beneficial uses in both rivers. Measured water-quality conditions in WY2009 and WY2010 exceeded these targets at one or more sites for the following constituents: water temperature, total phosphorus concentrations, total phosphorus loads, dissolved oxygen concentration, pH, and chlorophyll- a concentrations (WY2009 only). All measured total phosphorus concentrations in the Boise River near Parma exceeded the seasonal target of 0.07 milligram per liter. Data collected during the study show seasonal differences in all measured parameters. In particular, surprisingly high concentrations of chlorophyll- a were measured at all three main study sites in winter and early spring, likely due to changes in algal populations. Discharge conditions and dissolved orthophosphorus concentrations are key drivers for chlorophyll- a on a seasonal and annual basis on the Snake River. Discharge conditions and upstream periphyton growth are most likely the key drivers for chlorophyll- a in the Boise River. Phytoplankton growth is not limited or driven by nutrient availability in the Boise River. Lower discharges and minimal substrate disturbance in WY2010 in comparison with WY2009 may have caused prolonged and increased periphyton and macrophyte growth and a reduced amount of sloughed algae in suspension in the summer of WY2010. Chlorophyll- a measured in samples commonly is used as an indicator of sestonic algae biomass, but chlorophyll- a concentrations and fluorescence may not be the most appropriate surrogates for algae growth, eutrophication, and associated effects on beneficial uses. Assessment of the effects of algae growth on beneficial uses should evaluate not only sestonic algae, but also benthic algae and macrophytes. Alternatively, continuous monitoring of dissolved oxygen detects the influence of aquatic plant respiration for all types of algae and macrophytes and is likely a more direct measure of effects on beneficial uses such as aquatic habitat. Most measured water-quality parameters in the Snake River were statistically different upstream and downstream of the confluence with the Boise River. Higher concentrations and loads were measured at the downstream site (Snake River at Nyssa) than the upstream site (Snake River near Adrian) for total phosphorus, dissolved orthophosphorus, total nitrogen, dissolved nitrite and nitrate, suspended sediment, and turbidity. Higher dissolved oxygen concentrations and pH were measured at the upstream site (Snake River near Adrian) than the downstream site (Snake River at Nyssa). Contributions from the Boise River measured at Parma do not constitute all of the increase in nutrient and sediment loads in the Snake River between the upstream and downstream sites. Surrogate models were developed using a combination of continuously monitored variables to estimate concentrations of nutrients and suspended sediment when samples were not possible. The surrogate models explained from 66 to 95 percent of the variability in nutrient and suspended sediment concentrations, depending on the site and model. Although the surrogate models could not always represent event-based changes in modeled parameters, they generally were successful in representing seasonal and annual patterns. Over a longer period, the surrogate models could be a useful tool for measuring compliance with state and site-specific water-quality standards and TMDL targets, for representing daily and seasonal variability in constituents, and for assessing effects of phosphorus reduction measures within the watershed.

Idaho

Geologic map and profiles of the north wall of the Snake River Canyon, Thousand Springs and Niagara quadrangles, Idaho

The Snake River Plain is a broad, arcuate region of low relief that extends more than 300 mi across southern Idaho. The Snake River enters the plain near Idaho Falls and flows westward along the southern margin of the eastern Snake River Plain (fig. 1), a position mainly determined by the basaltic lava flows that erupted near the axis of the plain. The highly productive Snake River Plain aquifer north of the Snake River underlies most of the eastern plain. The aquifer is composed of basaltic rocks that are interbedded with fluvial and lacustrine sedimentary rocks. The top of the aquifer (water table) is typically less than 500 ft below the land surface but is deeper than 1,000 ft in a few areas. The Snake River has excavated a canyon into the nearly flat lying basaltic and sedimentary rocks of the eastern Snake River Plain aquifer, which discharges from the northern canyon wall as springs of variable size, spacing, and altitude. Geologic controls on springs are of importance because nearly 60 percent of the aquifer's discharge occurs as spring flow along the describes the geologic occurrence of springs along the northern wall of the Snake River canyon. This report is one of several that describes the geologic occurrence of springs along the northern wall of the Snake River canyon from Milner Dam to King Hill. To understand the local geologic controls on springs, the Water Resources Division of the U.S. Geological Survey initiated a geologic mapping project as part of their Snake River Plain Regional Aquifer System-Analysis Program. Objectives of the project were (1) to prepare a geologic map of a strip of land immediately north of the Snake River canyon, (2) to map the geology of the north canyon wall in profile, (3) to locate spring occurrences along the north side of the Snake River between Milner Sam and King Hill, and (4) to estimate spring discharge from the north wall of the canyon.

Idaho

Sediment transport in the lower Snake and Clearwater River Basins, Idaho and Washington, 2008–11

Sedimentation is an ongoing maintenance problem for reservoirs, limiting reservoir storage capacity and navigation. Because Lower Granite Reservoir in Washington is the most upstream of the four U.S. Army Corps of Engineers reservoirs on the lower Snake River, it receives and retains the largest amount of sediment. In 2008, in cooperation with the U.S. Army Corps of Engineers, the U.S. Geological Survey began a study to quantify sediment transport to Lower Granite Reservoir. Samples of suspended sediment and bedload were collected from streamgaging stations on the Snake River near Anatone, Washington, and the Clearwater River at Spalding, Idaho. Both streamgages were equipped with an acoustic Doppler velocity meter to evaluate the efficacy of acoustic backscatter for estimating suspended-sediment concentrations and transport. In 2009, sediment sampling was extended to 10 additional locations in tributary watersheds to help identify the dominant source areas for sediment delivery to Lower Granite Reservoir. Suspended-sediment samples were collected 9–15 times per year at each location to encompass a range of streamflow conditions and to capture significant hydrologic events such as peak snowmelt runoff and rain-on-snow. Bedload samples were collected at a subset of stations where the stream conditions were conducive for sampling, and when streamflow was sufficiently high for bedload transport. At most sampling locations, the concentration of suspended sediment varied by 3–5 orders of magnitude with concentrations directly correlated to streamflow. The largest median concentrations of suspended sediment (100 and 94 mg/L) were in samples collected from stations on the Palouse River at Hooper, Washington, and the Salmon River at White Bird, Idaho, respectively. The smallest median concentrations were in samples collected from the Selway River near Lowell, Idaho (11 mg/L), the Lochsa River near Lowell, Idaho (11 mg/L), the Clearwater River at Orofino, Idaho (13 mg/L), and the Middle Fork Clearwater River at Kooskia, Idaho (15 mg/L). The largest measured concentrations of suspended sediment (3,300 and 1,400 mg/L) during a rain-on-snow event in January 2011 were from samples collected at the Potlatch River near Spalding, Idaho, and the Palouse River at Hooper, Washington, respectively. Generally, samples collected from agricultural watersheds had a high percentage of silt and clay-sized suspended sediment, whereas samples collected from forested watersheds had a high percentage of sand. During water years 2009–11, Lower Granite Reservoir received about 10 million tons of suspended sediment from the combined loads of the Snake and Clearwater Rivers. The Snake River accounted for about 2.97 million tons per year (about 89 percent) of the total suspended sediment, 1.48 million tons per year (about 90 percent) of the suspended sand, and about 1.52 million tons per year (87 percent) of the suspended silt and clay. Of the suspended sediment transported to Lower Granite Reservoir, the Salmon River accounted for about 51 percent of the total suspended sediment, about 56 percent of the suspended sand, and about 44 percent of the suspended silt and clay. About 6.2 million tons (62 percent) of the sediment contributed to Lower Granite Reservoir during 2009–11 entered during water year 2011, which was characterized by an above average winter snowpack and sustained spring runoff. A comparison of historical data collected from the Snake River near Anatone with data collected during this study indicates that concentrations of total suspended sediment and suspended sand in the Snake River were significantly smaller during water years 1972–79 than during 2008–11. Most of the increased sediment content in the Snake River is attributable to an increase of sand-size material. During 1972–79, sand accounted for an average of 28 percent of the suspended-sediment load; during 2008–11, sand accounted for an average of 48 percent. Historical data from the Clearwater River at Spalding indicates that the concentrations of total suspended sediment collected during 1972–79 were not significantly different from the concentrations measured during this study. However, the suspended-sand concentrations in the Clearwater River were significantly smaller during 1972–79 than during 2008–11. The increase in suspended-sand concentrations in the Snake and Clearwater Rivers are probably attributable to numerous severe forest fires that burned large areas of central Idaho from 1980–2010. Acoustic backscatter from an acoustic Doppler velocity meter proved to be an effective method of estimating suspended-sediment concentration and load for most streamflow conditions in the Snake and Clearwater Rivers. Models based on acoustic backscatter were able to simulate most of the variability in suspended-sediment concentrations in the Clearwater River at Spalding (coefficient of determination [R 2 ]=0.93) and the Snake River near Anatone (R 2 =0.92). Acoustic backscatter seems to be especially effective for estimating suspended-sediment concentration and load over short (monthly and single storm event) and long (annual) time scales when sediment load is highly variable. However, during high streamflow events acoustic surrogate tools may be unable to capture the contribution of suspended sand moving near the bottom of the water column and thus, underestimate the total load of suspended sediment. At the stations where bedload was collected, the particle-size distribution at low streamflows typically was unimodal with sand comprising the dominant particle size. At higher streamflows and during peak bedload discharge, the particle size typically was bimodal and was comprised primarily of sand and coarse gravel. About 55,000 tons of bedload was discharged from the Snake River to Lower Granite Reservoir during water years 2009–11, about 0.62 percent of the total sediment load delivered by the Snake River. About 9,500 tons of bedload was discharged from the Clearwater River to Lower Granite Reservoir during 2009–11, about 0.83 percent of the total sediment load discharged by the Clearwater River during 2009–11.

Idaho;Washington

Contact rates with nesting birds before and after invasive snake removal: Estimating the effects of trap-based control

Invasive predators are responsible for almost 60% of all vertebrate extinctions worldwide with the most vulnerable faunas occurring on islands. The brown treesnake ( Boiga irregularis ) is a notorious invasive predator that caused the extirpation or extinction of most native forest birds on Guam. The success of avian reintroduction efforts on Guam will depend on whether snake-control techniques sufficiently reduce contact rates between brown treesnakes and reintroduced birds. Mouse-lure traps can successfully reduce brown treesnake populations at local scales. Over a 22-week period both with and without active snake removal, we evaluated snake-trap contact rates for mouse- and bird-lure traps. Bird-lure traps served as a proxy for reintroduced nesting birds. Overall, mouse-lure traps caught more snakes per trap night than did bird-lure traps. However, cameras revealed that bird-lure traps had a snake contact rate almost 15 times greater than the number of successfully captured snakes. Snakes that entered bird-lure traps tended to be larger and in better body condition and were mostly captured in bird-lure traps, despite numerous adjacent mouse-lure traps. Traps placed along grid edges caught more snakes than interior traps, suggesting continuous immigration into the trapping grid within which bird-lure traps were located. Contact between snakes and bird-lure traps was equivalent before and after snake removal, suggesting mouse-lure traps did not adequately reduce the density of snakes that posed a risk to birds, at least at the timescale of this project. This study provides evidence that some snakes exhibit prey selectivity for live birds over live mouse lures. Reliance on a single control tool and lure may be inadequate for support of avian reintroductions and could lead to unintended harvest-driven trait changes of this invasive predator.

NeoBiota

Major reorganization of the Snake River modulated by passage of the Yellowstone Hotspot

The details and mechanisms for Neogene river reorganization in the U.S. Pacific Northwest and northern Rocky Mountains have been debated for over a century with key implications for how tectonic and volcanic systems modulate topographic development. To evaluate paleo-drainage networks, we produced an expansive data set and provenance analysis of detrital zircon U-Pb ages from Miocene to Pleistocene fluvial strata along proposed proto-Snake and Columbia River pathways. Statistical comparisons of Miocene-Pliocene detrital zircon spectra do not support previously hypothesized drainage routes of the Snake River. We use detrital zircon unmixing models to test prior Snake River routes against a newly hypothesized route, in which the Snake River circumnavigated the northern Rocky Mountains and entered the Columbia Basin from the northeast prior to incision of Hells Canyon. Our proposed ancestral Snake River route best matches detrital zircon age spectra throughout the region. Furthermore, this northerly Snake River route satisfies and provides context for shifts in the sedimentology and fish faunal assemblages of the western Snake River Plain and Columbia Basin through Miocene−Pliocene time. We posit that eastward migration of the Yellowstone Hotspot and its effect on thermally induced buoyancy and topographic uplift, coupled with volcanic densification of the eastern Snake River Plain lithosphere, are the primary mechanisms for drainage reorganization and that the eastern and western Snake River Plain were isolated from one another until the early Pliocene. Following this basin integration, the substantial increase in drainage area to the western Snake River Plain likely overtopped a bedrock threshold that previously contained Lake Idaho, which led to incision of Hells Canyon and establishment of the modern Snake and Columbia River drainage network.

Alberta, British Columbia, Idaho, Montana, Oregon,

Geologic map and profiles of the north wall of the Snake River Canyon, Bliss, Hagerman, and Tuttle quadrangles, Idaho

The Snake River Plain is a broad, arcuate region of low relief that extends more than 300 mi across southern Idaho. The Snake River enters the plain near Idaho Falls and flows westward along the southern margin of the eastern Snake River Plain (fig. 1), a position mainly determined by the basaltic lava flows that erupted near the axis of the plain. The highly productive Snake River Plain aquifer north of the Snake River underlies most of the eastern plain. The aquifer is composed of basaltic rocks that are interbedded with fluvial and lacustrine sedimentary rocks. The top of the aquifer (water table) is typically less than 500 ft below the land surface, but is deeper than 1,000 ft in a few areas. The Snake River has excavated a canyon into the nearly flat-lying basaltic and sedimentary rocks of the eastern Snake River Plain between Milner Dam and King Hill (fig. 2), a distance of almost 90 mi. For much of its length the canyon wall as springs of variable size, spacing, and altitude. Geologic controls on springs are of importance because nearly 60 percent of the aquifer's discharge occurs as spring flow along this reach of the canyon. This report is one of several that describes the geologic occurrence of springs along the northern wall of the Snake River canyon from Milner Dam to King Hill (fig. 1). To understand the local geologic controls on springs, the Water Resources Division of the U.S. Geological Survey initiated a geologic mapping project as part of their Snake River Plain Regional Aquifer System-Analysis Program. Objectives of the project were (1) to prepare a geologic map of a strip of land immediately north of the Snake River canyon, (2) to map the geology of the north canyon wall in profile, (3) to locate spring occurrences along the north side of the Snake River between Milner Dam and King Hill, and (4) to estimate spring discharge from the north wall of the canyon.

Idaho

Preliminary geochemical assessment of water in selected streams, springs, and caves in the Upper Baker and Snake Creek drainages in Great Basin National Park, Nevada, 2009

Water in caves, discharging from springs, and flowing in streams in the upper Baker and Snake Creek drainages are important natural resources in Great Basin National Park, Nevada. Water and rock samples were collected from 15 sites during February 2009 as part of a series of investigations evaluating the potential for water resource depletion in the park resulting from the current and proposed groundwater withdrawals. This report summarizes general geochemical characteristics of water samples collected from the upper Baker and Snake Creek drainages for eventual use in evaluating possible hydrologic connections between the streams and selected caves and springs discharging in limestone terrain within each watershed. Generally, water discharging from selected springs in the upper Baker and Snake Creek watersheds is relatively young and, in some cases, has similar chemical characteristics to water collected from associated streams. In the upper Baker Creek drainage, geochemical data suggest possible hydrologic connections between Baker Creek and selected springs and caves along it. The analytical results for water samples collected from Wheelers Deep and Model Caves show characteristics similar to those from Baker Creek, suggesting a hydrologic connection between the creek and caves, a finding previously documented by other researchers. Generally, geochemical evidence does not support a connection between water flowing in Pole Canyon Creek to that in Model Cave, at least not to any appreciable extent. The water sample collected from Rosethorn Spring had relatively high concentrations of many of the constituents sampled as part of this study. This finding was expected as the water from the spring travelled through alluvium prior to being discharged at the surface and, as a result, was provided the opportunity to interact with soil minerals with which it came into contact. Isotopic evidence does not preclude a connection between Baker Creek and the water discharging from Rosethorn Spring. The residence time of water discharging into the caves and from selected springs sampled as part of this study ranged from 10 to 25 years. Within the upper Snake Creek drainage, the results of this study show geochemical similarities between Snake Creek and Outhouse Spring, Spring Creek Spring, and Squirrel Spring Cave. The strontium isotope ratio ( 87 Sr/ 86 Sr) for intrusive rock samples representative of the Snake Creek drainage were similar to carbonate rock samples. The water sample collected from Snake Creek at the pipeline discharge point had lower strontium concentrations than the sample downstream and a similar 87 Sr/ 86 Sr value as the carbonate and intrusive rocks. The chemistry of the water sample was considered representative of upstream conditions in Snake Creek and indicates minimal influence of rock dissolution. The results of this study suggest that water discharging from Outlet Spring is not hydrologically connected to Snake Creek but rather is recharged at high altitude(s) within the Snake Creek drainage. These findings for Outlet Spring largely stem from the relatively high specific conductance and chloride concentration, the lightest deuterium (δD) and oxygen-18 (δ 18 O) values, and the longest calculated residence time (60 to 90 years) relative to any other sample collected as part of this study. With the exception of water sampled from Outlet Spring, the residence time of water discharging into Squirrel Spring Cave and selected springs in the upper Snake Creek drainage was less than 30 years.

Nevada

Mammalian lures monitored with time-lapse cameras increase detection of pythons and other snakes

Background Enhancing detection of cryptic snakes is critical for the development of conservation and management strategies; yet, finding methods that provide adequate detection remains challenging. Issues with detecting snakes can be particularly problematic for some species, like the invasive Burmese python ( Python bivittatus ) in the Florida Everglades. Methods Using multiple survey methods, we predicted that our ability to detect pythons, larger snakes and all other snakes would be enhanced with the use of live mammalian lures (domesticated rabbits; Oryctolagus cuniculus ). Specifically, we used visual surveys, python detection dogs, and time-lapse game cameras to determine if domesticated rabbits were an effective lure. Results Time-lapse game cameras detected almost 40 times more snakes ( n = 375, treatment = 245, control = 130) than visual surveys ( n = 10). We recorded 21 independent detections of pythons at treatment pens (with lures) and one detection at a control pen (without lures). In addition, we found larger snakes, and all other snakes were 165% and 74% more likely to be detected at treatment pens compared to control pens, respectively. Time-lapse cameras detected almost 40 times more snakes than visual surveys; we did not detect any pythons with python detection dogs. Conclusions Our study presents compelling evidence that the detection of snakes is improved by coupling live mammalian lures with time-lapse game cameras. Although the identification of smaller snake species was limited, this was due to pixel resolution, which could be improved by changing the camera focal length. For larger snakes with individually distinctive patterns, this method could potentially be used to identify unique individuals and thus allow researchers to estimate population dynamics.

Florida

Historical and current perspectives on fish assemblages of the Snake River, Idaho and Wyoming

The Snake River is the tenth longest river in the United States, extending 1,667 km from its origin in Yellowstone National Park in western Wyoming to its union with the Columbia River at Pasco, Washington. Historically, the main-stem Snake River upstream from the Hells Canyon Complex supported at least 26 native fish species, including anadromous stocks of Chinook salmon Oncorhynchus tshawytscha, steelhead O. mykiss, Pacific lamprey Lampetra tridentata, and white sturgeon Acipenser transmontanus. Of these anadromous species, only the white sturgeon remains in the Snake River between the Hells Canyon Complex and Shoshone Falls. Today, much of the Snake River has been transformed into a river with numerous impoundments and flow diversions, increased pollutant loads, and elevated water temperatures. Current (1993-2002) fish assemblage collections from 15 sites along the Snake River and Henrys Fork contained 35 fish species, including 16 alien species. Many of these alien species such as catfish (Ictaluridae), carp (Cyprinidae), and sunfish (Centrarchidae) are adapted for warmwater impounded habitats. Currently, the Snake River supports 19 native species. An index of biotic integrity (IBI), developed to evaluate large rivers in the Northwest, was used to evaluate recent (1993-2002) fish collections from the Snake River and Henrys Fork in southern Idaho and western Wyoming. Index of biotic integrity site scores and component metrics revealed a decline in biotic integrity from upstream to downstream in both the Snake River and Henrys Fork. Two distinct groups of sites were evident that correspond to a range of IBI scores-an upper Snake River and Henrys Fork group with relatively high biotic integrity (mean IBI scores of 46-84) and a lower Snake River group with low biotic integrity (mean IBI scores of 10-29). Sites located in the lower Snake River exhibited fish assemblages that reflect poor-quality habitat where coldwater and sensitive species are rare or absent, and where tolerant, less desirable species predominate. Increases in percentages of agricultural land, total number of diversions, and number of constructed channels were strongly associated with these decreasing IBI scores.

American Fisheries Society Symposium

Object detection-assisted workflow facilitates cryptic snake monitoring

Camera traps are an important tool used to study rare and cryptic animals, including snakes. Time-lapse photography can be particularly useful for studying snakes that often fail to trigger a camera's infrared motion sensor due to their ectothermic nature. However, the large datasets produced by time-lapse photography require labor-intensive classification, limiting their use in large-scale studies. While many artificial intelligence-based object detection models are effective at identifying mammals in images, their ability to detect snakes is unproven. Here, we used camera data to evaluate the efficacy of an object detection model to rapidly and accurately detect snakes. We classified images manually to the species level and compared this with a hybrid review workflow where the model removed blank images followed by a manual review. Using a ≥0.05 model confidence threshold, our hybrid review workflow correctly identified 94.5% of blank images, completed image classification 6× faster, and detected large (>66 cm) snakes as well as manual review. Conversely, the hybrid review method often failed to detect all instances of a snake in a string of images and detected fewer small (<66 cm) snakes than manual review. However, most relevant ecological information requires only a single detection in a sequence of images, and study design changes could likely improve the detection of smaller snakes. Our findings suggest that an object detection-assisted hybrid workflow can greatly reduce time spent manually classifying data-heavy time-lapse snake studies and facilitate ecological monitoring for large snakes.

Florida

Behavioral plasticity in detection height of an invasive, arboreal snake based on size, condition, and prey

Context Animals may adjust their behavior in predictable ways to balance tradeoffs between resource acquisition and survival or fecundity. Microhabitat selection based on individual traits or environmental conditions is one measure of risk–reward tradeoffs by individuals. Aims We used data from observational and manipulative studies to investigate whether an arboreal snake (brown treesnake, Boiga irregularis ) had context-dependent behavior based on the relationship between estimated prey availability, body condition, size, and detection height (microhabitat use) in two Mariana Islands. Methods We used observational data collected in four study sites and data from a manipulative study that we collected over a 5-year period. The observational data focused on four sites with different counts of three prey types, including lizards, birds, and small mammals. During the manipulative study we removed snakes, which resulted in increased prey counts over time. Using these two approaches, we tested whether prey counts predicted body condition and then evaluated how prey counts, snake size, and snake condition interactively predicted the detection height of captured individuals. Key results We found that body condition was greater at sites or in years with greater prey counts across both the observational and manipulative studies. We also found that snakes displayed differential microhabitat use based on both their condition and size. Larger snakes tended to be detected lower than smaller snakes, but only at sites or during years with few bird or small mammal counts. Snakes at sites with greater mammal and bird counts had a positive relationship between size and detection height. Snakes with greater condition scores tended to be detected higher irrespective of size, but this was also dependent on prey counts. At sites with low bird counts, snakes that were in better condition tended to be closer to the ground. Conclusions Brown treesnakes modified microhabitat use based on their condition, size, and the number or type of prey available. Our findings were consistent with a hypothesis that they optimized habitat use to secure food resources and maximize survival. Implications Context-dependent behavioral plasticity may be an important consideration for management of reptiles for population control or growth.

Wildlife Research

Habitat fragmentation effects on annual survival of the federally protected eastern indigo snake

The eastern indigo snake (Drymarchon couperi) is a federally listed species, most recently threatened by habitat loss and habitat degradation. In an effort to estimate snake survival, a total of 103 individuals (59 males, 44 females) were followed using radio-tracking from January 1998 to March 2004 in three landscape types that had increasing levels of habitat fragmentation: (1) conservation cores; (2) conservation areas along highways; (3) suburbs. Because of a large number of radio-tracking locations underground for which the state of snakes (i.e. alive or dead) could not be assessed, we employed a multistate approach to model snake apparent survival and encounter probability of live and dead snakes. We predicted that male snakes in suburbs would have the lowest annual survival. We found a transmitter implantation effect on snake encounter probability, as snakes implanted on a given occasion had a lower encounter probability on the next visit compared with snakes not implanted on the previous occasion. Our results indicated that adult eastern indigo snakes have relatively high survival in conservation core areas, but greatly reduced survival in conservation areas along highways and in suburbs. These findings indicate that habitat fragmentation is likely to be the critical factor for species' persistence.

Animal Conservation

TaqMan real-time polymerase chain reaction for detection of Ophidiomyces ophiodiicola , the fungus associated with snake fungal disease

Background Fungal skin infections associated with Ophidiomyces ophiodiicola , a member of the Chrysosporium anamorph of Nannizziopsis vriesii (CANV) complex, have been linked to an increasing number of cases of snake fungal disease (SFD) in captive snakes around the world and in wild snake populations in eastern North America. The emergence of SFD in both captive and wild situations has led to an increased need for tools to better diagnose and study the disease. Results We developed two TaqMan real-time polymerase chain reaction (PCR) assays to rapidly detect O. ophiodiicola in clinical samples. One assay targets the internal transcribed spacer region (ITS) of the fungal genome while the other targets the more variable intergenic spacer region (IGS). The PCR assays were qualified using skin samples collected from 50 snakes for which O. ophiodiicola had been previously detected by culture, 20 snakes with gross skin lesions suggestive of SFD but which were culture-negative for O. ophiodiicola , and 16 snakes with no clinical signs of infection. Both assays performed equivalently and proved to be more sensitive than traditional culture methods, detecting O. ophiodiicola in 98% of the culture-positive samples and in 40% of the culture-negative snakes that had clinical signs of SFD. In addition, the assays did not cross-react with a panel of 28 fungal species that are closely related to O. ophiodiicola or that commonly occur on the skin of snakes. The assays did, however, indicate that some asymptomatic snakes (~6%) may harbor low levels of the fungus, and that PCR should be paired with histology when a definitive diagnosis is required. Conclusions These assays represent the first published methods to detect O. ophiodiicola by real-time PCR. The ITS assay has great utility for assisting with SFD diagnoses whereas the IGS assay offers a valuable tool for research-based applications. Keywords: Chrysosporium anamorph of Nannizziopsis vriesii (CANV); Emerging disease; Ophidiomyces ophiodiicola ; Real-time PCR; Snake fungal disease

BMC Veterinary Research

Potential effects of groundwater pumping on water levels, phreatophytes, and spring discharges in Spring and Snake Valleys, White Pine County, Nevada, and adjacent areas in Nevada and Utah

Assessing hydrologic effects of developing groundwater supplies in Snake Valley required numerical, groundwater-flow models to estimate the timing and magnitude of capture from streams, springs, wetlands, and phreatophytes. Estimating general water-table decline also required groundwater simulation. The hydraulic conductivity of basin fill and transmissivity of basement-rock distributions in Spring and Snake Valleys were refined by calibrating a steady state, three-dimensional, MODFLOW model of the carbonate-rock province to predevelopment conditions. Hydraulic properties and boundary conditions were defined primarily from the Regional Aquifer-System Analysis (RASA) model except in Spring and Snake Valleys. This locally refined model was referred to as the Great Basin National Park calibration (GBNP-C) model. Groundwater discharges from phreatophyte areas and springs in Spring and Snake Valleys were simulated as specified discharges in the GBNP-C model. These discharges equaled mapped rates and measured discharges, respectively. Recharge, hydraulic conductivity, and transmissivity were distributed throughout Spring and Snake Valleys with pilot points and interpolated to model cells with kriging in geologically similar areas. Transmissivity of the basement rocks was estimated because thickness is correlated poorly with transmissivity. Transmissivity estimates were constrained by aquifer-test results in basin-fill and carbonate-rock aquifers. Recharge, hydraulic conductivity, and transmissivity distributions of the GBNP-C model were estimated by minimizing a weighted composite, sum-of-squares objective function that included measurement and Tikhonov regularization observations. Tikhonov regularization observations were equations that defined preferred relations between the pilot points. Measured water levels, water levels that were simulated with RASA, depth-to-water beneath distributed groundwater and spring discharges, land-surface altitudes, spring discharge at Fish Springs, and changes in discharge on selected creek reaches were measurement observations. The effects of uncertain distributed groundwater-discharge estimates in Spring and Snake Valleys on transmissivity estimates were bounded with alternative models. Annual distributed groundwater discharges from Spring and Snake Valleys in the alternative models totaled 151,000 and 227,000 acre-feet, respectively and represented 20 percent differences from the 187,000 acre-feet per year that discharges from the GBNP-C model. Transmissivity estimates in the basin fill between Baker and Big Springs changed less than 50 percent between the two alternative models. Potential effects of pumping from Snake Valley were estimated with the Great Basin National Park predictive (GBNP-P) model, which is a transient groundwater-flow model. The hydraulic conductivity of basin fill and transmissivity of basement rock were the GBNP-C model distributions. Specific yields were defined from aquifer tests. Captures of distributed groundwater and spring discharges were simulated in the GBNP-P model using a combination of well and drain packages in MODFLOW. Simulated groundwater captures could not exceed measured groundwater-discharge rates. Four groundwater-development scenarios were investigated where total annual withdrawals ranged from 10,000 to 50,000 acre-feet during a 200-year pumping period. Four additional scenarios also were simulated that added the effects of existing pumping in Snake Valley. Potential groundwater pumping locations were limited to nine proposed points of diversion. Results are presented as maps of groundwater capture and drawdown, time series of drawdowns and discharges from selected wells, and time series of discharge reductions from selected springs and control volumes. Simulated drawdown propagation was attenuated where groundwater discharge could be captured. General patterns of groundwater capture and water-table declines were similar for all scenarios. Simulated drawdowns greater than 1 ft propagated outside of Spring and Snake Valleys after 200 years of pumping in all scenarios.

Scientific Investigations Report