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The conservation genetics juggling act: Integrating genetics and ecology, science and policy

The field of conservation genetics, when properly implemented, is a constant juggling act integrating molecular genetics, ecology, and demography with applied aspects concerning managing declining species or implementing conservation laws and policies. This young field has grown substantially since the 1980’s following development of the polymerase chain reaction and now into the genomics era. Our lab has “grown up” with the field, having worked on these issues for over three decades. Our multi-disciplinary approach entails understanding the behavior and ecology of species as well as the underlying processes that contribute to genetic viability. Taking this holistic approach provides a comprehensive understanding of factors that influence species persistence and evolutionary potential while considering annual challenges that occur throughout their life cycle. As a federal lab, we are often addressing the needs of the U.S. Fish and Wildlife Service in their efforts to list, de-list or recover species. Nevertheless, there remains an overall communication gap between research geneticists and biologists who are charged with implementing their results. Therefore, we outline the need for a National Center for Small Population Biology to ameliorate this problem and provide organizations charged with making status decisions firmer ground from which to make their critical decisions.

Evolutionary Applications

Assessment of general health of fishes collected at selected sites in the Great Lakes Basin In 2012

During the past decade, there has been a substantive increase in the detection of “emerging contaminants”, defined as a new substance, chemical, or metabolite in the environment; or a legacy substance with a newly expanded distribution, altered release, or a newly recognized effect (such as endocrine disruption). Emerging contaminants include substances such as biogenic hormones (human and animal), brominated flame retardants, pharmaceuticals, personal care products, plasticizers, current use pesticides, detergents, and nanoparticles. These contaminants are frequently not regulated or inadequately regulated by state or Federal water quality programs. Information about the toxicity of these substances to fish and wildlife resources is generally limited, compared to more highly regulated contaminants, and some classes have been shown to cause affects (for example feminization of male fish, immunomodulation) that are not evaluated via traditional toxicity testing protocols. As a result, these compounds may pose a substantial, but currently poorly documented threat to aquatic ecosystems. Failure to identify and understand the impacts of these emerging contaminants on fish and wildlife resources may result in deleterious impacts to Great Lakes resources that can result in adverse ecological, economic and recreational consequences. The U. S. Fish and Wildlife Service received funding through the Great Lakes Restoration Initiative (GLRI) for an Early Warning Program to detect and identify emerging contaminants and to evaluate the effects of these contaminants on fish and wildlife. The U.S. Geological Survey (WV Cooperative Fish and Wildlife Research Unit and National Fish Health Research Laboratory, Leetown Science Center) developed and implemented a biological effects monitoring protocol to assist in this program. Fish collections and measurements of biomarkers of exposure in Fall 2010 and Spring 2011 occurred at individual sites within select Areas of Concern (AOCs). They provided an assessment of the utility of the suite of biomarkers and also identified sites for more in-depth analyses. Selected areas are characterized as areas with known emerging contaminants, sensitive or listed species, areas downstream from municipal wastewater discharges or receiving waters for industrial facilities, and/or areas susceptible to agricultural or urban contamination, or harbors or ports. The results of the 2010- 2011 studies were summarized in Blazer et al. 2014 a, b, c; Braham et al. in review and Blazer et al. in review.

Great Lakes

Seventy-five years of science—The U.S. Geological Survey’s Western Fisheries Research Center

As of January 2010, 75 years have elapsed since Dr. Frederic Fish initiated the pioneering research program that would evolve into today’s Western Fisheries Research Center (WFRC). Fish began his research working alone in the basement of the recently opened Fisheries Biological Laboratory on Lake Union in Seattle, Washington. WFRC’s research began under the aegis of the U.S. Fish and Wildlife Service and ends its first 75 years as part of the U.S. Geological Survey with a staff of more than 150 biologists and support personnel and a heritage of fundamental research that has made important contributions to our understanding of the biology and ecology of the economically important fish and fish populations of the Nation. Although the current staff may rarely stop to think about it, WFRC’s antecedents extend many years into the past and are intimately involved with the history of fisheries conservation in the Western United States. Thus, WFRC Director Lyman Thorsteinson asked me to write the story of this laboratory “while there are still a few of you around who were here for some of the earlier years” to document the rich history and culture of WFRC by recognizing its many famous scientists and their achievements. This historyalso would help document WFRC’s research ‘footprint’ in the Western United States and its strategic directions. Center Director Thorsteinson concluded that WFRC’s heritage told by an emeritus scientist also would add a texture of legitimacy based on personal knowledge that will all-to-soon be lost to the WFRC and to the USGS. The WFRC story is important for the future as well as for historical reasons. It describes how we got to the place we are today by documenting the origin, original mission, and our evolving role in response to the constantly changing technical information requirements of new environmental legislation and organizational decision-making. The WFRC research program owes its existence to the policy requirements of Federal conservation legislation originating with the construction of Grand Coulee Dam in 1933. The research program was shaped by laws enacted in subsequent years such as the Federal Water Pollution Control Act (1972), National Environmental Policy Act (1973), Endangered Species Act (1974), and Northwest Power Planning Act (1980), to name only a few. The WFRC has not been constrained by direct management or regulatory responsibility for a particular fishery (such as providing sustainable catch limits data to a resource management structure). Thus, WFRC has been able to concentrate on scientific pursuits and information needs required by contemporary environmental legislation. Over the years, we have pioneered in several important areas of fisheries research including the diagnoses and control of diseases in economically important fish, effects of environmental alterations on the physiological quality and survival of Pacific salmon released from federal mitigation hatcheries, applications in biotelemetry, and the bioenergetics of predator-prey interactions in the Columbia River. The WFRC of today is a widely distributed organization in the Western United States. Knowledge of the historical connections and accomplishments of our predecessors is important beyond the sense of pride and unity it instills in the WFRC family of today. For example, a discerning reader will note the evolution of WFRC’s research from a single disciplinary focus (early era—hatchery disease problems), to multiple disciplines (middle to late era—species, populations, habitats; threatened and endangered species), to the present era (multidisciplinary and with increasing process focus). For the benefit of the current WFRC staff, more emphasis has been placed on the early years rather than on the present day because people are quite naturally more familiar with the recent past than with the research done during the first decades of WFRC’s existence. By every rational measure, the WFRC has evolved into a fisheries research organization well positioned to provide the biological information needed to support the continued conservation and management of our Nation’s living aquatic natural resources. The high standard of excellence that connects WFRC’s past to our present research program provides a firm foundation on which to base the work yet to be done. In another 75 years, WFRC will undoubtedly be a very different place than it is today, but its evolution will be forever rooted in the story of the research and of the people related here. More about the diverse fisheries research projects WFRC scientists are conducting today is available at WFRC’s website: http://wfrc.usgs.gov/.

Washington

USGS Alaska Tissue Archival Projects: An update on FY02 activities

The banking of environmental specimens under cryogenic conditions for future retrospective analysis has been recognized for many years as an important part of environmental monitoring programs. Since 1987, the Alaska Marine Mammal Tissue Archival Project (AMMTAP) has been collecting tissue samples from marine mammals for archival in the National Biomonitoring Specimen Bank (NBSB) at the National Institute of Standards and Technology (NIST) in Gaithersburg, Maryland, USA. The USGS, Alaska Biological Science Center (ABSC), the NOAA Fisheries, Office of Protected Resources (NMFS), and the NIST conduct this partnership project, which began under the Mineral Management Service (MMS) Outer Continental Shelf Environmental Assessment Program. MMS remains the primary client agency for the AMMTAP providing programmatic guidance and review. The purpose of the project is to collect tissue samples from Alaska marine mammals and to store these specimens under the best conditions so that they can be analyzed for environmental contaminants and other constituents. A substantial part of the sample collection is from Arctic species and, since most of the animals sampled are from Alaska Native subsistence harvests, the project relies on cooperation and collaboration with several Alaska Native organizations and local governmental agencies. Although a substantial amount of recent research has been conducted on contaminants in Alaskan marine mammals, few data exists on colonial seabirds nesting in Alaska. Like marine mammals, seabirds are an important group of upper trophic level marine organisms with a potential for accumulating lipophilic contaminants and are identified by MMS as species of interest for monitoring activities. More than 95% of the seabirds breeding in the continental United States nest at colonies in the Bering and Chukchi seas and Gulf of Alaska (see USFWS 1992). Realizing the value of colonial seabirds in environmental monitoring and the lack of recent data from Alaskan seabird colonies, the U.S. Fish and Wildlife Service Alaska Maritime National Wildlife Refuge (USFWS-AMNWR), the U.S. Geological Survey Biological Resources Division (USGS-BRD), and the National Institute of Standards and Technology (NIST) initiated the Seabird Tissue Archival and Monitoring Project (STAMP) in 1998. The project was designed as a 100-year-long program to monitor long-term trends in environmental quality by collecting eggs at nesting colonies using standardized protocols, banking the egg contents under conditions that ensure chemical stability during long-term (decadal) storage, and analyzing subsamples of the stored material to establish baseline levels for persistent bioaccumulative contaminants (e.g., chlorinated pesticides, PCBs, mercury).

Conference Paper

Coastal mapping programs at the U.S. Fish and Wildlife Service's National Wetlands Research Center

Over the past 10 years, the U.S. Fish and Wildlife Service's (FWS) National Wetlands Research Center (center; formerly the National Coastal Ecosystems Team) has been continuously involved in the production of maps for use by coastal decision makers. The types of maps produced by the center have been national, regional, or local in scope depending on user needs. Map scales have ranged from 1:24,000 to 1:250,000. Themes depicted have included biological resources, including wetlands and seagrasses; upland habitat or land use; water resources such as water quality, bathymetry, and salinity; cultural features such as ownership, archaeological sites, and dredge-spoil disposal areas; and soils and landforms. We present overviews on the various mapping programs of the center. We highlight efforts such as the ecological inventories of the Atlantic, Gulf, and Pacific coasts; the ecological characterization atlases of the Gulf of Mexico; and the large scale (1:24,000) habitat maps of various coastal regions of the United States. Center methods and techniques are discussed, including the collaborative efforts between the center and FWS's National Wetlands Inventory for updating wetland maps and adding upland and seagrass bed delineations to inventory maps. We also make recommendations for future coastal ecosystem mapping programs that use conventional and automated mapping methodologies, such as geographic information systems and image processing.

Biological Report

USFWS selected geographic analyses

The geographic information system (GIS) used by the National Wetlands Research Center (NWRC) of the U.S. Fish and Wildlife Service has been valuable in assisting natural resource managers in planning and managing coastal fish and wildlife resources. In the past 5 years, NWRC has conducted about 60 studies employing this technology. Applications have ranged from simple natural resource inventories to documentation of trends in wetland changes and complex predictive modeling of impacts of harbor expansion on biological resources. Maps, tables, and data bases from these studies have been presented before local, State, and Congressional committees as well as numerous scientific and public organizations. This paper describes and summarizes selected GIS projects related to resource inventories, analyses of wetland change, and cartographic modeling of coastal areas of the United States.

Conference Paper

Attitudinal survey component of the study Quantity, quality, and support for research in the U.S. Fish and Wildlife Service: An organizational assessment : Report of methods and frequencies

The U.S. Fish and Wildlife Service (FWS) is responsible for managing the Nation’s fish and wildlife resources so that these trust resources are preserved for the present and future use and enjoyment of the citizens of the United States. The FWS achieves this mission by managing many programs. These include the national system of refuges and fish hatcheries, Fish and Wildlife Management Assistance Offices, migratory birds program, law enforcement, and working with tribal, state, and other Federal agencies to ensure protection of threatened and endangered species. Another role of the FWS is consulting with tribal, state, and other Federal agencies and private sector interests on the best conservation management practices consistent with Federal law. Each of these activities requires a workforce that is recognized for its professionalism, dedication to public service, and command of expert knowledge. Recognition for expert knowledge in fish and wildlife conservation is demonstrated, in part, when FWS personnel direct, conduct, or report research that is well-designed to answer questions of importance for natural resource management. The data reported in this document are one part of a three-part study of the status of organizational support for research in FWS, which was commissioned by the Directorate of the FWS. Funding for this study was provided by the FWS, and the Science Support Program of the U.S. Geological Survey (USGS). In 1994, the biological research functions of the FWS were transferred to the National Biological Survey, and subsequently into the USGS. This transfer was principally accomplished by moving whole research units from one agency to another. The result was that some employees whose positions were involved with research were not transferred. In addition, some research, information, and management needs of the FWS have continued to be met by studies conducted within the FWS itself. Although the FWS relies on the USGS and others for most basic research investigations, the FWS also conducts its own studies to meet management needs. Because it is vital for FWS employees to be able to conduct such tactical and applied research tasks in a timely manner, the agency must promote the culture necessary to support and encourage these activities. Such research activities are spread widely across the various programs of the FWS, and there is presently no collective, formal, or systematic record of planned or existing research activities. In commissioning this organizational assessment research, the Directorate of the FWS recognized that it would be to the advantage of the agency to more fully understand its research capacity. To develop a clearer picture of the nature, extent, quality, and degree of administrative support available for conducting research within the FWS, investigations have been undertaken to: 1. identify positions in the FWS that may include, in whole or in part, a component of scientific research; 2. identify organizational units within the FWS that may conduct research as a significant portion of their mission; and 3. assess the attitudes of employees and managers about the obstacles and opportunities for scientific research existing within the FWS by using a scaled-response survey instrument. The findings presented in this report represent the basic results derived from the attitude assessment survey conducted in the last quarter of 2004. The findings set forth in this report are the frequency distributions for each question in the survey instrument for all respondents. The only statistics provided are descriptive in character - namely, means and associated standard deviations.

Open-File Report

Interim report on the scientific investigations in the Animas River watershed, Colorado to facilitate remediation decisions by the U.S. Bureau of Land Management and the U.S. Forest Service, March 29, 2000 meeting, Denver, Colo.

INTRODUCTION The joint U.S. Department of the Interior and U.S. Department of Agriculture Abandoned Mine Lands Initiative (AMLI) was developed as a collaborative effort between the Federal land management agencies (FLMA, that is the U.S. Bureau of Land Management and the U.S. Forest Service) and the U.S. Geological Survey (USGS) in 1996. The stated goal of the AML Initiative was to develop a strategy for gathering and communicating the scientific information needed to develop effective and cost-efficient remediation of abandoned mines within the framework of a watershed. Four primary objectives of the AMLI are to: 1. Provide the scientific information needed (in the short-term) by the FLMAs to make decisions related to the design and implementation of cleanup actions, 2. Develop a multi-disciplined, multi-division approach that integrates geologic, hydrologic, geochemical and ecological information into a knowledge base for sound decision making, 3. Transfer technologies developed within the scientific programs of the USGS to the field and demonstrate their suitability to solve real, practical problems, and 4. Establish working relationships among involved members of land management and regulatory agencies within the framework of a watershed approach to the cleanup of abandoned mines. Long-term process-based research, including development of analytical tools, is recognized as being critical to the long-term success in remediating watersheds impacted by historical mining activities (AML 5-year plan, http://amli.usgs.gov/amli). In a meeting of Federal agencies (U.S. Bureau of Land Management [BLM], U.S. Bureau of Reclamation [BOR], U.S. National Park Service [NPS], U.S. Forest Service [USFS], the U.S. Environmental Protection Agency [EPA], the U.S. Fish and Wildlife Service [F&WS]), and State agencies (Colorado Division of Public Health and Environment, Colorado Division of Mines and Geology), several watersheds were examined within the state whose water quality was presumed to be impacted by historical mining activities. The Animas River watershed (fig. 1) was selected by the State and Federal agencies as one of two watersheds in the U.S. to be studied in detail by the USGS in the AML Initiative. Beginning in October 1997, each of the four Divisions of the USGS (Water Resources, Geologic, Biological Resources, and National Mapping) initiated a collaborative integrated science study of the watershed. Funds were provided from USGS base funding to each of the four Divisions in response to the priorities set by Congressional action and within the flexibility provided by the budgetary framework funding individual research programs. The AML Initiative provides for a five-year focused scientific effort in the two watersheds with final synthesis of the scientific results from each to be published in 2001. Publications are released on the AML web site on a regular basis (http://amli.usgs.gov/amli). On March 29, 2000, the USGS hosted a meeting for the BLM and USFS to discuss remediation options that were under consideration for the summer of 2000. The purpose of this report is to provide an overview of the scientific rational provided by the USGS to meet objective one above, and to summarize our preliminary interpretations of our data. Additional information from sites on private lands have been collected by the State of Colorado, EPA, and the ARSG. Unfortunately, these data have not been fully supplied to the USGS so our conclusions are based only upon our data. These interpretations provide science-based constraints on possible remediation options to be considered by the FLMA, the State, and local property owners in the Animas River watershed. The report is presented in outline format to facilitate discussion of remediation options at the March 29, 2000 meeting. Not all historical mining sites within the watershed are on public lands. This should not be construed to be a final report of the USGS

Denver

The ecology of Humboldt Bay, California, an estuarine profile

Synthesizes data on the ecological relationships and functions of the estuary, including information on physical aspects of the bay environment; describes the biotic communities and their relationships; provides management considerations in terms including environmental concerns; and identifies research and management information gaps. Portions of the bay are managed as a national wildlife refuge. Management issues for this ecosystem include loss of habitat and degradation of the environment by additional industrial development and nonpoint source pollution. -from Authors

California

[Book review] Endangered Birds: Management techniques for preserving threatened species

This volume contains the proceedings of a symposium held at the University of Wisconsin-Madison, during 17-20 August 1977. In the words of the book's editor, Stanley Temple, the symposium "offered a unique opportunity for leading scientists from around the world to meet and share their experiences with endangered birds." That this objective was achieved is indicated by a list of contributors standing as a "Who's Who" in endangered species research and management. This symposium was jointly sponsored by the International Council for Bird Preservation, World Wildlife Fund, National Audubon Society, New York Zoological Society, and U.S. Fish and Wildlife Service. Fifty-one papers by 53 contributors are listed under 10 topics: endangered bird problems and the concept of managing threatened species; increasing reproductive effort and success by reducing nest-site limitations; alleviating problems of competition, predation, parasitism, and disease; supplemental feeding and manipulation of feeding ecology; manipulating aspects of nesting biology; captive breeding of endangered birds; genetic aspects of managing dwindling populations; reintroducing endangered birds to the wild; integrated approaches to management of endangered birds; and summary. A 14-page index provides convenient reference to subjects in the text.

The Auk

Brine contamination to aquatic resources from oil and gas development in the Williston Basin, United States

The Williston Basin, which includes parts of Montana, North Dakota, and South Dakota in the United States and the provinces of Manitoba and Saskatchewan in Canada, has been a leading domestic oil and gas producing region for more than one-half a century. Currently, there are renewed efforts to develop oil and gas resources from deep geologic formations, spurred by advances in recovery technologies and economic incentives associated with the price of oil. Domestic oil and gas production has many economic benefits and provides a means for the United States to fulfill a part of domestic energy demands; however, environmental hazards can be associated with this type of energy production in the Williston Basin, particularly to aquatic resources (surface water and shallow groundwater) by extremely saline water, or brine, which is produced with oil and gas. The primary source of concern is the migration of brine from buried reserve pits that were used to store produced water during recovery operations; however, there also are considerable risks of brine release from pipeline failures, poor infrastructure construction, and flow-back water from hydraulic fracturing associated with modern oilfield operations. During 2008, a multidisciplinary (biology, geology, water) team of U.S. Geological Survey researchers was assembled to investigate potential energy production effects in the Williston Basin. Researchers from the U.S. Geological Survey participated in field tours and met with representatives from county, State, tribal, and Federal agencies to identify information needs and focus research objectives. Common questions from agency personnel, especially those from the U.S. Fish and Wildlife Service, were “are the brine plumes (plumes of brine-contaminated groundwater) from abandoned oil wells affecting wetlands on Waterfowl Production Areas and National Wildlife Refuges?” and “are newer wells related to Bakken and Three Forks development different than the older, abandoned wells (in terms of potential for affecting aquatic resources)?” Of special concern were the wetland habitats of the ecologically important Prairie Pothole Region, which overlays a part of the Williston Basin and is recognized for the production of a majority of North America’s migratory waterfowl. On the basis of the concerns raised by on-the-ground land managers, as well as findings from previous research, a comprehensive study was developed with the following goals: summarize existing information pertaining to oil and gas production and aquatic resources in the Williston Basin; assess brine plume migration from new and previously studied sites in the Prairie Pothole Region; perform a regional, spatial evaluation of oil and gas production activities and aquatic resources; assess the potential for brine contamination to wetlands and streams; and hold a decision analysis workshop with key stakeholders to discuss issues pertaining to oil and gas production and environmental effects and to identify information gaps and research needs. This report represents an initial, multidisciplinary evaluation of measured and potential environmental effects associated with oil and gas production in the Williston Basin and Prairie Pothole Region. Throughout this report there are reviews of current knowledge, and discussions relating to data gaps and research needs. On the basis of the information presented, future research needs include: regional geophysical and water-quality assessments to establish baselines for current conditions and estimate the extent of previous brine contamination, investigations into the direct effects of brine to biotic communities, and evaluations to identify the most effective techniques to mitigate brine contamination.

Montana, North Dakota

Columbia Environmental Research Center

The U.S. Geological Survey Columbia Environmental Research Center performs research to solve challenging environmental problems related to contaminants and habitat alterations in aquatic and terrestrial ecosystems. The research is interdisciplinary and pursued through partnerships within the U.S. Geological Survey and with national, international, state, and local agencies; nongovernmental organizations; and universities. Research is prioritized to provide science to the U.S. Department of the Interior and other natural resource management agencies to inform rehabilitation of degraded habitats and imperiled fish and wildlife populations. The Columbia Environmental Research Center was established in 1966 in Columbia, Missouri, as the U.S. Fish and Wildlife Service’s Fish Pesticide Research Laboratory; the Columbia Environmental Research Center was incorporated into the U.S. Geological Survey in 1996. The U.S. Geological Survey’s staff of 130 includes 90 scientists of which one-half have advanced degrees in ecology, toxicology, biology, biochemistry, chemistry, hydrology, geology, and information technology.

Missouri

An international borderland of concern: Conservation of biodiversity in the Lower Rio Grande Valley

The Lower Rio Grande Valley (LRGV) of southern Texas is located on the United States-Mexico borderland and represents a 240-kilometer (150-mile) linear stretch that ends at the Gulf of Mexico. The LRGV represents a unique transition between temperate and tropical conditions and, as such, sustains an exceptionally high diversity of plants and animals—some of them found in few, or no other, places in the United States. Examples include Leopardus pardalis albescens (northern ocelot) and Falco femoralis septentrionalis (northern aplomado falcon)—both endangered in the United States and emblematic of the LRGV. The U.S. Fish and Wildlife Service (USFWS) manages three national wildlife refuges (Santa Ana, Lower Rio Grande Valley, and Laguna Atascosa) that together make up the South Texas Refuge Complex, which actively conserves biodiversity in about 76,006 hectares (187,815.5 acres) of native riparian and upland habitats in the LRGV. These diminished habitats harbor many rare, threatened, and endangered species. This report updates the widely used 1988 USFWS biological report titled “Tamaulipan Brushland of the Lower Rio Grande Valley of South Texas: Description, Human Impacts, and Management Options” by synthesizing nearly 400 peer-reviewed scientific publications that have resulted from biological and sociological research conducted specifically in the four Texas counties of the LRGV in the past nearly 30 years. This report has three goals: (1) synthesize scientific insights gained since 1988 related to the biology and management of the LRGV and its unique biota, focusing on flora and fauna of greatest conservation concern; (2) update ongoing challenges facing Federal and State agencies and organizations that focus on conservation or key natural resources in the LRGV; and (3) redefine conservation opportunities and land-acquisition strategies that are feasible and appropriate today, given the many new and expanding constraints that challenge conservation activities in the LRGV. The LRGV faces every contemporary conservation challenge of the 21st century, but ongoing human population growth and its associated demands, international border issues, and oil, gas, and alternative energy development dominate impacts that affect conservation in the LRGV. Continued careful syntheses of existing and future information collected in the LRGV are needed on many biological and sociological topics to guide conservation activities. Quick response will no doubt be necessary to face contemporary and difficult-to-predict challenges such as climate change, diminished water availability and quality, spread of invasive species, and habitat loss and fragmentation. Complexities of a guarded international borderland add pressure to small patches of native habitat that remain in many places of the LRGV, particularly along the Rio Grande. Large connected corridors of restored native habitat could be the best option to maintain, and even enhance, the exceptional biodiversity of the LRGV in the face of exceptional human demand.

Texas

Final project memorandum: sea-level rise modeling handbook: resource guide for resource managers, engineers, and scientists

Coastal wetlands of the Southeastern United States are undergoing retreat and migration from increasing tidal inundation and saltwater intrusion attributed to climate variability and sea-level rise. Much of the literature describing potential sea-level rise projections and modeling predictions are found in peer-reviewed academic journals or government technical reports largely suited to reading by other Ph.D. scientists who are more familiar or engaged in the climate change debate. Various sea-level rise and coastal wetland models have been developed and applied of different designs and scales of spatial and temporal complexity for predicting habitat and environmental change that have not heretofore been synthesized to aid natural resource managers of their utility and limitations. Training sessions were conducted with Federal land managers with U.S. Fish and Wildlife Service, National Park Service, and NOAA National Estuarine Research Reserves as well as state partners and nongovernmental organizations across the northern Gulf Coast from Florida to Texas to educate and to evaluate user needs and understanding of concepts, data, and modeling tools for projecting sea-level rise and its impact on coastal habitats and wildlife. As a result, this handbook was constructed from these training and feedback sessions with coastal managers and biologists of published decision-support tools and simulation models for sea-level rise and climate change assessments. A simplified tabular context was developed listing the various kinds of decision-support tools and ecological models along with criteria to distinguish the source, scale, and quality of information input and geographic data sets, physical and biological constraints and relationships, datum characteristics of water and land elevation components, utility options for setting sea-level rise and climate change scenarios, and ease or difficulty of storing, displaying, or interpreting model output. The handbook is designed to be a primer to understanding sea-level rise and a practical synthesis of the current state of knowledge and modeling tools as a resource guide for DOl land management needs and facilitating Landscape Conservation Cooperative (LCC) research and conservation initiatives.

Conference Paper

A river system to watch: documenting the effects of saltcedar ( Tamarix spp.) biocontrol in the Virgin River valley

Throughout riparian areas of the southwestern United States, non-native saltcedar (also known as tamarisk; Tamarix spp.) can form dense, monotypic stands and is often reported to have detrimental effects on native plants and habitat quality (Everitt 1980; Shafroth et al. 2005). Natural resource managers of these riparian areas spend considerable time and resources controlling saltcedar using a variety of techniques, including chemical (Duncan and McDaniel 1998), mechanical, and burning methods (Shafroth et al. 2005). Approximately one billion dollars are spent each year on river restoration projects nationally (Bernhardt et al. 2005), and a majority of these projects focus on invasive species control in the Southwest (Follstad Shah et al. 2007). A technique that has drawn much attention is the use of the saltcedar leaf beetle ( Diorhabda spp.), a specialist herbivore, as biological control of saltcedar (Lewis et al. 2003). Research testing was conducted with beetles housed in secure enclosures in six states in 1998 and 1999 (Dudley et al. 2001), followed by open release at some of those sites starting in 2001 (DeLoach et al. 2004). By 2005, full-scale saltcedar biocontrol was implemented in 13 states, led by the USDA Animal and Plant Health Inspection Service (APHIS), the agency that oversees biological control programs, and with the participation and support of the U.S. Fish and Wildlife Service (USFWS). Despite the widespread application of Diorhabda , however, only limited research has quantified the consequences (benefits and costs) on biotic communities and ecosystem services. Alterations to riparian areas caused by various non-native species control activities have the potential to affect a variety of habitat types used by wildlife (Bateman et al. 2008a); processes like water availability, fluvial deposition, and erosion; and the establishment of other non-native species (Carruthers and D'Antonio 2005, Shafroth et al. 2005, DeLoach et al. 2006). Similarly, biocontrol is expected to modify riparian ecosystems, and it is imperative to document and evaluate both the environmental benefits and the potential costs of this tamarisk management method.

Virgin River

Design and testing of a mesocosm-scale habitat for culturing the endangered Devils Hole Pupfish

aptive propagation of desert spring fishes, whether for conservation or research, is often difficult, given the unique and often challenging environments these fish utilize in nature. High temperatures, low dissolved oxygen, minimal water flow, and highly variable lighting are some conditions a researcher might need to recreate to simulate their natural environments. Here we describe a mesocosm-scale habitat created to maintain hybrid Devils Hole × Ash Meadows Amargosa Pupfish ( Cyprinodon diabolis × C. nevadensis mionectes ) under conditions similar to those found in Devils Hole, Nevada. This 13,000-L system utilized flow control and natural processes to maintain these conditions rather than utilizing complex and expensive automation. We designed a rotating solar collector to control natural sunlight, a biological reactor to consume oxygen while buffering water quality, and a reverse-daylight photosynthesis sump system to stabilize nighttime pH and swings in dissolved oxygen levels. This system successfully controlled many desired parameters and helped inform development of a larger, more permanent desert fish conservation facility at the U.S. Fish and Wildlife Service’s Ash Meadows National Wildlife Refuge, Nevada. For others who need to raise fish from unique habitats, many components of the scalable and modular design of this system can be adapted at reasonable cost.

North American Journal of Aquaculture

Restoring Pacific Lamprey in the Umpqua River Basin of Oregon: A workshop summary

The Umpqua River Basin in southwestern Oregon (Figure 1) is part of the lands inhabited by the Cow Creek Band of Umpqua Tribe of Indians and an area of active co-management authority. This Basin supports a unique fish fauna, including important populations of Pacific salmon ( Oncorhynchus spp.) and steelhead ( O. mykiss ), and other native fishes that are endemic to the region (Mims et al. 2018). Among these species, the Pacific Lamprey ( Entosphenus tridentatus ) is one of the most unique, representing an ancient lineage of jawless fishes that long predates the evolution of any other species of fish in the basin (Clemens et al. 2017, 2021a). The Pacific Lamprey also represents an important cultural and food resource that features prominently in the indigenous practices of the Cow Creek Band of Umpqua Tribe of Indians. This report provides a select summary of topics related to the conservation of Pacific Lamprey in the Umpqua River Basin. Many of the topics addressed herein were discussed in a workshop co-hosted by the Cow Creek Band of Umpqua Tribe of Indians and the U.S. Geological Survey in April of 2022. This workshop was focused on threats to Pacific Lamprey in fresh waters of the basin. The workshop highlighted science conducted over the past 15 years by local managers and researchers to understand and address these threats and provide new information relevant to the conservation and restoration of Pacific Lamprey. Attendees included staff from the Coquille Indian Tribe, Yakama Nation, Columbia River Inter-Tribal Fish Commission, Partnership for Umpqua Rivers, Rogue River Watershed Council, Curry Watersheds Council, PacifiCorp, Trout Unlimited, Jackson Soil and Water Conservation District, Oregon Department of Fish and Wildlife, Bureau of Land Management, U.S. Fish and Wildlife Service, and U.S. Forest Service. This report provides an overview of this workshop and recent science and provides an overview of potential future efforts that could inform restoration of Pacific Lamprey. This summary is organized into four sections that relate to the main topics of the workshop: 1) The physical habitat template (stream flow, instream wood, sediment, and water temperature), 2) movement barriers and reservoirs, 3) biological invasions, and 4) climate adaptation. These do not represent an exhaustive list of topics related to conservation of Pacific Lamprey in the Umpqua Basin.

Oregon

Ecoregions of California

Ecoregions denote areas of general similarity in ecosystems and in the type, quality, and quantity of environmental resources. They are designed to serve as a spatial framework for the research, assessment, management, and monitoring of ecosystems and ecosystem components. By recognizing the spatial differences in the capacities and potentials of ecosystems, ecoregions stratify the environment by its probable response to disturbance (Bryce and others, 1999). These general purpose regions are critical for structuring and implementing ecosystem management strategies across Federal agencies, State agencies, and nongovernment organizations that are responsible for different types of resources in the same geographical areas (Omernik and others, 2000). The approach used to compile this map is based on the premise that ecological regions are hierarchical and can be identified through the analysis of the spatial patterns and the composition of biotic and abiotic phenomena that affect or reflect differences in ecosystem quality and integrity (Wiken, 1986; Omernik, 1987, 1995). These phenomena include geology, physiography, vegetation, climate, soils, land use, wildlife, and hydrology. The relative importance of each characteristic varies from one ecological region to another regardless of the hierarchical level. A Roman numeral hierarchical scheme has been adopted for different levels of ecological regions. Level I is the coarsest level, dividing North America into 15 ecological regions. Level II divides the continent into 50 regions (Commission for Environmental Cooperation Working Group, 1997, map revised 2006). At level III, the continental United States contains 105 ecoregions and the conterminous United States has 85 ecoregions (U.S. Environmental Protection Agency, 2013). Level IV, depicted here for California, is a further refinement of level III ecoregions. Explanations of the methods used to define these ecoregions are given in Omernik (1995), Omernik and others (2000), and Omernik and Griffith (2014). California has great ecological and biological diversity. The State contains offshore islands and coastal lowlands, large alluvial valleys, forested mountain ranges, deserts, and various aquatic habitats. There are 13 level III ecoregions and 177 level IV ecoregions in California and most continue into ecologically similar parts of adjacent States of the United States or Mexico (Bryce and others, 2003; Thorson and others, 2003; Griffith and others, 2014). The California ecoregion map was compiled at a scale of 1:250,000. It revises and subdivides an earlier national ecoregion map that was originally compiled at a smaller scale (Omernik, 1987; U.S. Environmental Protection Agency, 2013). This poster is the result of a collaborative project primarily between U.S. Environmental Protection Agency (USEPA) Region IX, USEPA National Health and Environmental Effects Research Laboratory (Corvallis, Oregon), California Department of Fish and Wildlife (DFW), U.S. Department of Agriculture (USDA)–Natural Resources Conservation Service (NRCS), U.S. Department of the Interior–Geological Survey (USGS), and other State of California agencies and universities. The project is associated with interagency efforts to develop a common framework of ecological regions (McMahon and others, 2001). Reaching that objective requires recognition of the differences in the conceptual approaches and mapping methodologies applied to develop the most common ecoregion-type frameworks, including those developed by the USDA–Forest Service (Bailey and others, 1994; Miles and Goudy, 1997; Cleland and others, 2007), the USEPA (Omernik 1987, 1995), and the NRCS (U.S. Department of Agriculture–Soil Conservation Service, 1981; U.S. Department of Agriculture–Natural Resources Conservation Service, 2006). As each of these frameworks is further refined, their differences are becoming less discernible. Regional collaborative projects such as this one in California, where some agreement has been reached among multiple resource-management agencies, are a step toward attaining consensus and consistency in ecoregion frameworks for the entire nation.

California