Fish Immunology: J. S. Stolen, D. P. Anderson, and W. B. van Muiswinkel, editors; 1985; Elsevier Science Publishing Co. Inc. 485 pages
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N-sodium-N-chloro-rho-toluenesulfonamide (chloramine-T) effectively controls bacterial gill disease (BGD) in cultured fishes, BGD, a common disease of hatchery-reared salmonids, causes more fish losses than any other disease among these species, This study describes a liquid chromatographic (LC) method that is capable of direct, simultaneous analysis of chloramine-T and its primary degradation product, rho-toluenesulfonamide (rho-TSA), in water. The procedure involves reversed-phase (C-18) LC analysis with ion suppression, using 0.01 M phosphate buffer at pH 3. The mobile phase is phosphate buffer-acetonitrile (60 + 40) at 1 mL/min. Both chemicals can be detected with a UV spectrophotometer at 229 nm; the method is linear up to 40 mg, chloramine-T or rho-TSA/L. Mean recoveries were 96.4 +/- 6.1% for water samples fortified with 0.03 mg chloramine-T/L and 95.3 +/- 4.6% for water samples fortified with 0.005 mg rho-TSA/L. Limits of detection without sample enrichment for chloramine-T and rho-TSA are 0.01 mg/L and 0.001 mg/L, respectively.
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Some of our most well known fish viruses are now members of a newly recognized genus within the virus family Rhabdoviridae. As a member of the rhabdovirus study group of the International Committee for Virus Taxonomy (ICTV), I have been involved for the last five years in working towards updated and improved taxonomic classification of fish rhabdoviruses. The major fruit of this effort has been the establishment of a new genus that includes many, but not all, rhabdoviruses of fish hosts. Although this new genus was officially accepted by the ICTV in July of 1998, the continuing delay in publication of the seventh report of the ICTV has meant that there is still no official notification of this change. This article is meant to explain the new genus to all interested fish health professionals.