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Physiological indices of seawater readiness in postspawning steelhead kelts

Management goals to improve the recovery of steelhead (Oncorhynchus mykiss) stocks at risk of extinction include increasing the proportion of postspawning fish that survive and spawn again. To be successful, postspawning steelhead (kelts) migrating downstream to the ocean must prepare physiologically and physically for a seawater transition. We sampled blood, gill filaments, and evaluated the external condition of migrating kelts from an ESA-listed population in the Snake/Columbia River system over two consecutive years to evaluate their physiological readiness for transition to seawater. We chose attributes often considered as measures of preparation for seawater in juveniles, including gill Na + ,K + ATPase activity, plasma electrolytes and hormones to consider factors related to external condition, size and sex. We found kelts in good external condition had plasma profiles similar to downstream-migrating smolts. In addition, we found more than 80% of kelts ranked in good external condition had smolt-like body silvering. We compared measures from migrating kelts with samples obtained from hatchery fish at the time of spawning to confirm that Na + , K + ATPase activity in kelts was significantly elevated over spawning fish. We found significant differences in gill Na + , K + ATPase activity in migrating kelts between the years of sampling, but little indication of influence of fish condition. We conclude that the postspawning steelhead sampled exhibited a suite of behaviours, condition and physiology characteristic of fish prepared for successful transition to a seawater environment.

Idaho;Washington

The complimentary role of lentic and lotic habitats for Arctic grayling in a complex stream-lake network in Arctic Alaska

Lakes can be important to stream dwelling fishes, yet how individuals exploit habitat heterogeneity across complex stream-lake networks is poorly understood. Furthermore, despite growing awareness that intermittent streams are widely used by fish, studies documenting use of seasonally accessible lakes remain scarce. We studied Arctic grayling (Thymallus arcticus) in a small seasonally flowing (June – October) stream-lake network in Alaska using PIT telemetry. Overall, 70% of fish visited two lakes, 8% used a single lake, and 22% used only stream reaches. We identified four distinct behavioral patterns that differed in dominant macrohabitat used (deep-lake, shallow-lake, or stream reaches), entry-day into the network, and mobility. Some juvenile fish spent the entire summer in a shallow seasonally frozen lake (average 71 days), whereas others demonstrated prospecting behavior and only entered the stream channel briefly in September. Another group included adult and juvenile fish that were highly mobile, moving up to 27 km while in the 3-km stream-lake network, and used stream reaches extensively (average 59 days). Lentic and lotic habitats served differing roles for individuals, some fish occupied stream reaches as summer foraging habitat and other individuals used them as migration corridors to access lakes. Our study emphasizes the importance of considering stream-lake connectivity in stream fish assessments, even to shallow seasonally frozen habitats not widely recognized as important. Furthermore, we demonstrate that individuals may use temporary aquatic habitats in complex and changing ways across ontogeny that are not captured by typical classifications of fish movement behavior.

Alaska

Native fish abundance and habitat selection changes in the presence of nonnative piscivores

We compared abundance patterns and developed resource selection models for imperilled native southwestern (USA) fishes in the presence and absence of Black Bass ( Micropterus spp.) to evaluate how fishes alter their selection for habitats when sympatric with a nonnative piscivore. We collected data using snorkel surveys and in-stream habitat sampling in Fossil Creek (AZ), upstream (native fish only) and downstream (native and nonnative fish) of a fish barrier. The abundance of all Roundtail Chub ( Gila robusta ), small (≤127 mm total length [TL]; vulnerable to predation) Sonora Sucker ( Catostomus insignis ) and Speckled Dace ( Rhinichthys osculus ) was significantly reduced, but the abundance of both small and large (>127 mm TL; invulnerable to predation) Desert Sucker ( Catostomus clarkii ) was similar in sampling reaches with and without Black Bass. When sympatric with Black Bass, small Roundtail Chub increased their selection for riffles by 2.57 times and small Desert Sucker reduce their selection for pools by 6.90 times while also selecting for faster flow velocity and finer substrates in lotic mesohabitats. Large native fishes altered selection least, notwithstanding an increased selection for canopy cover in sampling reaches with Black Bass. Observed shifts in resource selection are consistent with predator avoidance strategies. Our study highlights the behavioural consequences of nonnative piscivores on native fish communities and stresses the importance of maintaining lotic mesohabitats as potential refugia for vulnerable native fishes when nonnative piscivores are present.

Arizona

Rapid colonisation post-displacement contributes to native fish resilience

Native freshwater fish are experiencing global declines. Determining what drives native fish resilience to disturbance is crucial to understanding their persistence in the face of multiple stressors. Fish colonisation ability may be one factor affecting population resilience after disturbance. We conducted displacement experiments in headwater streams in Wyoming, USA, to evaluate mottled sculpin ( Cottus bairdii ) and mountain sucker ( Catostomus platyrhynchus ) colonisation ability. Specifically, we (1) determined whether fish could colonise sites rapidly after displacement, (2) evaluated site-level factors affecting colonisation, and (3) compared species-level differences in movement and colonisation capabilities. Mountain sucker recovered to pre-displacement abundances within 6–11 weeks, but mottled sculpin were still at slightly reduced abundances. For both species, the majority of colonists were unmarked new individuals and size–structure was similar to pre-displacement size–structure. Fish colonisation was best predicted by pre-displacement abundance and an interaction between per cent riparian cover and species identity. The slower colonisation rate of mottled sculpin may relate to movement ability as average daily movement rate and movement extent were significantly greater for mountain sucker. Our results demonstrate that colonisation is one mechanism allowing fish populations to be resilient in the face of disturbance and that species' traits provide insight into fish colonisation capabilities. Experimental approaches provide mechanistic insight into colonisation dynamics, enhancing our understanding of native fish resilience in degraded stream ecosystems and their response to restoration actions.

Wyoming

Life-history connections to long-term fish population trends in a species-rich temperate river

Fishes exhibit a diverse range of traits encompassing life-history strategies, feeding behaviours and spawning behaviours. These traits mediate fish population responses to changing environmental conditions such as those caused by anthropogenic stressors. The Conasauga River, located in northwestern Georgia and southeastern Tennessee, USA, hosts a diverse assemblage of over 75 species of freshwater fish, some of which are locally or regionally endemic, and many of which are imperilled. Annual monitoring data have shown population declines in multiple fish species of conservation concern in the Conasauga River since at least the 1990s, raising the possibility that other taxa could be declining as well. We quantified temporal changes in fish communities at six shoal sites sampled annually in most years from 1996 to 2022, and asked whether species traits hypothesized to underlie population vulnerability to environmental alteration were correlated with species-specific trends for 32 taxa. We estimated that total counts of fish in annual samples declined by ~2% per year, although declines were uneven among species and generally greater for less abundant taxa. Tests for species traits corresponding to temporal population trends provided evidence that crevice-spawning minnows and smaller-bodied taxa had steeper declines compared with broadcast spawners and larger, longer-lived, more fecund taxa. Lower abundance, reliance on a particular habitat feature, and life-history traits that may limit population resilience to disturbance may all prove useful for identifying riverine fishes at particular risk of future population decline.

Georgia

Predicting invasiveness of freshwater fishes imported into North America: Regional differences in models and outcomes

Biological invasions driven by international trade heighten the urgency for development of invasion risk models, as the traits and parameters that consistently predict successful invasion remain unresolved. For four regions of North America that include parts of the United States and Canada (Sacramento-San Joaquin River Basins, Lower Colorado River Basin, Great Lakes Region, Mid-Atlantic Region), we construct and compare classification tree models to reveal robust predictors for the establishment and ecological impact stages of freshwater fish invasion. We subsequently apply the models to identify invasive fish species in trade and conduct pathway analyses to determine which trades (aquarium, biological supply, live bait, live food, water garden) and source continents pose the greatest risk to each region. Model results differed by invasion stage and region. Across regions, establishment models shared climate-related predictors including climate match and temperature tolerance. Three of the four impact models contained prior establishment success. The greatest number of species (548) were predicted to establish in the Sacramento-San Joaquin while the fewest (5) were predicted to establish in the Mid-Atlantic. Forty species were predicted to establish in multiple regions, five of which were also predicted to have high impact. The aquarium trade and Asia supplied the most species predicted to establish. Taken together, the results highlight region-specific models, indicating no universal model predicts invasion. Climate-related and prior establishment variables were most useful to risk assessments. The regional models, and identified high-risk pathways and potential invaders, could be applied to prevent future fish invasions in North America.

Laurentian Great Lakes Region, Lower Colorado Rive

Thermal transfer rate is slower in bigger fish: How does body size affect response time of small, implantable temperature recording tags?

The recent miniaturisation of implantable temperature recording tags has made measuring the water temperatures fish experience in the wild possible, but there may be a body size-dependent delay in implanted tag response time to changes in external temperature. To determine whether fish body size affects the response rate of implanted temperature tags, we implanted 20 Salvelinus fontinalis (127–228 mm fork length (FL), 15.1–120.4 g) with temperature recording tags and subjected them to rapid temperature changes (±8°C in less than 2 seconds) in the laboratory. We found that thermal transfer rates, and the lag in temperature tag response rate, was positively correlated with fish size, but the direction of temperature change (colder or warmer) had no significant effect. In fish exposed to a slower rate of temperature change (2°C h −1 ) implanted tags did not show a response lag. Understanding the limitations of this important technology is crucial to determining the utility of the data it produces and its ability to accurately measure fish thermal experience in the wild.

Ecology of Freshwater Fish

Hierarchical faunal filters: An approach to assessing effects of habitat and nonnative species on native fishes

Understanding factors related to the occurrence of species across multiple spatial and temporal scales is critical to the conservation and management of native fishes, especially for those species at the edge of their natural distribution. We used the concept of hierarchical faunal filters to provide a framework for investigating the influence of habitat characteristics and normative piscivores on the occurrence of 10 native fishes in streams of the North Platte River watershed in Wyoming. Three faunal filters were developed for each species: (i) large-scale biogeographic, (ii) local abiotic, and (iii) biotic. The large-scale biogeographic filter, composed of elevation and stream-size thresholds, was used to determine the boundaries within which each species might be expected to occur. Then, a local abiotic filter (i.e., habitat associations), developed using binary logistic-regression analysis, estimated the probability of occurrence of each species from features such as maximum depth, substrate composition, submergent aquatic vegetation, woody debris, and channel morphology (e.g., amount of pool habitat). Lastly, a biotic faunal filter was developed using binary logistic regression to estimate the probability of occurrence of each species relative to the abundance of nonnative piscivores in a reach. Conceptualising fish assemblages within a framework of hierarchical faunal filters is simple and logical, helps direct conservation and management activities, and provides important information on the ecology of fishes in the western Great Plains of North America. ?? Blackwell Munksgaard, 2004.

Ecology of Freshwater Fish

Instream cover and shade mediate avian predation on trout in semi-natural streams

Piscivory by birds can be significant, particularly on fish in small streams and during seasonal low flow when available cover from predators can be limited. Yet, how varying amounts of cover may change the extent of predation mortality from avian predators on fish is not clear. We evaluated size-selective survival of coastal cutthroat trout (Oncorhynchus clarkii clarkii) in replicated semi-natural stream sections. These sections provided high (0.01 m2 of cover per m2 of stream) or low (0.002 m2 of cover per m2 of stream) levels of instream cover available to trout and were closed to emigration. Each fish was individually tagged, allowing us to track retention of individuals during the course of the 36-day experiment, which we attributed to survival from predators, because fish had no other way to leave the streams. Although other avian predators may have been active in our system and not detected, the only predator observed was the belted kingfisher Megaceryle alcyon, which is known to prey heavily on fish. In both treatments, trout >20.4 cm were not preyed upon indicating an increased ability to prey upon on smaller individuals. Increased availability of cover improved survival of trout by 12% in high relative to low cover stream sections. Trout also survived better in stream sections with greater shade, a factor we could not control in our system. Collectively, these findings indicate that instream cover and shade from avian predators can play an important role in driving survival of fish in small streams or during periods of low flow.

Oregon

Seasonal shifts in diel vertical migrations by lake-dwelling coastal cutthroat trout, Oncorhynchus clarkii clarkii, reflect thermal regimes and prey distributions

Lakes provide important habitat for salmonids that may use them as a primary feeding area between periods of reproduction. The seasonal changes in vertical thermal structure in lakes can affect the distribution of salmonids on seasonal and diel time scales as they search for, consume, and digest prey that also exploits the water column's distribution of food, temperature and light. Our goal was to analyse the vertical distribution of wild, native coastal cutthroat trout ( Oncorhynchus clarkii clarkii ) in Lake Washington on daily and seasonal time scales. This lake is stratified in the summer and isothermal in winter, allowing us to compare vertical movements between periods with and without thermal structure in water 50 m deep. We predicted that trout would be deeper in the water column during stratified months and shallower during isothermal months, and shallower at night than in the day. Overall, the trout showed these patterns in the depths and temperatures they occupied, tending to be within or below the thermocline in the summer but not in the coolest water available, and closer to the surface when the lake was isothermal. The trout were also closer to the surface at night and deeper during the day. The vertical range of these diel movements shifted with the seasons–deepest in October, as the thermocline deepened and weakened, and shallowest in January when the lake was isothermal. These seasonal and diel vertical distribution patterns by the trout optimise metabolism for growth, and facilitate feeding on planktivorous fishes that also show seasonal and diel vertical distribution changes.

Washington

Effects of constant and cyclical thermal regimes on growth and feeding of juvenile cutthroat trout of variable sizes

The effects of constant (12, 18, and 24 °C) and cyclical (daily variation of 15–21 and 12–24 °C) thermal regimes on the growth and feeding of Lahontan cutthroat trout ( Oncorhynchus clarki henshawi ) of variable sizes were examined. Higher constant temperatures (i.e., 24 °C) and more variable daily temperatures (i.e., 12–24 °C daily cycle) negatively affected growth rates. As fish mass increased (from 0.24 to 15.52 g) the effects of different thermal regimes on mass growth became more pronounced. Following 14 days exposure to the thermal regimes, feeding rates of individual fish were assessed during acute exposure (40 min) to test temperatures of 12, 18, and 24 °C. Feeding rate was depressed during acute exposure to 24 °C, but was not significantly affected by the preceding thermal regime. Our results indicate that even brief daily exposure to higher temperatures (e.g., 24 °C) can have considerable sublethal effects on cutthroat trout, and that fish size should be considered when examining the effects of temperature.

Ecology of Freshwater Fish

Environmental factors regulating the recruitment of walleye Sander vitreus and white bass Morone chrysops in irrigation reservoirs

Understanding the environmental factors that regulate fish recruitment is essential for effective management of fisheries. Generally, first-year survival, and therefore recruitment, is inherently less consistent in systems with high intra- and interannual variability. Irrigation reservoirs display sporadic patterns of annual drawdown, which can pose a substantial challenge to recruitment of fishes. We developed species-specific models using an 18-year data set compiled from state and federal agencies to investigate variables that regulate the recruitment of walleye Sander vitreus and white bass Morone chrysops in irrigation reservoirs in south-west Nebraska, USA. The candidate model set for walleye included only abiotic variables (water-level elevation, minimum daily air temperature during winter prior to hatching, annual precipitation, spring warming rate and May reservoir discharge), and the candidate model set for white bass included primarily biotic variables (catch per unit effort (CPUE) of black crappie Pomoxis nigromaculatus , CPUE of age-0 walleye, CPUE of bluegill Lepomis macrochirus and CPUE of age-3 and older white bass), each of which had a greater relative importance than the single abiotic variable (minimum daily air temperature during winter after hatching). Our findings improve the understanding of the recruitment of fishes in irrigation reservoirs and the relative roles of abiotic and biotic factors.

Nebraska

Links between riparian landcover, instream environment and fish assemblages in headwater streams of south-eastern Brazil

We hypothesised and tested a hierarchical organisation model where riparian landcover would influence bank composition and light availability, which in turn would influence instream environments and control fish assemblages. The study was conducted during the dry season in 11 headwater tributaries of the Sorocaba River in the upper Paraná River Basin, south-eastern Brazil. We focused on seven environmental factors each represented by one or multiple environmental variables and seven fish functional traits each represented by two or more classes. Multivariate direct gradient analyses suggested that riparian zone landcover can be considered a higher level causal factor in a network of relations that control instream characteristics and fish assemblages. Our results provide a framework for a hierarchical conceptual model that identifies singular and collective influences of variables from different scales on each other and ultimately on different aspects related to stream fish functional composition. This conceptual model is focused on the relationships between riparian landcover and instream variables as causal factors on the organisation of stream fish assemblages. Our results can also be viewed as a model for headwater stream management in that landcover can be manipulated to influence factors such as bank composition, substrates and water quality, whereas fish assemblage composition can be used as indicators to monitor the success of such efforts.

Sorocaba River Basin

Brook trout (Salvelinus fontinalis) movement and survival after removal of two dams on the West Branch of the Wolf River, Wisconsin

Dam removals allow fish to access habitats that may provide ecological benefits and risks, but the extent of fish movements through former dam sites has not been thoroughly evaluated for many species. We installed stationary PIT antennas in 2016 and 2017 to evaluate movements and survival of brook trout Salvelinus fontinalis in the West Branch of the Wolf River (WBWR) in central Wisconsin following removal of two dams and channel modifications designed to promote fish movement. These changes provided access to lacustrine habitats that might provide suitable winter habitat or act as ecological sinks. We used multistate models to estimate transition probabilities between river sections, to determine whether brook trout: (a) moved between multiple river sections and (b) entered lacustrine habitats as seasonal refuges, but eventually returned to lotic habitat. We also used a Cormack-Jolly-Seber model to evaluate whether apparent survival of brook trout in the WBWR was comparable to other populations. Few fish moved among river sections or used lacustrine habitat (<5% of tagged fish); most brook trout remained in sections where they were initially tagged, potentially due to quality habitat located throughout the river. Like other studies, brook trout in the WBWR appear to experience high mortality based on low number of detections, few physical recaptures and an estimated eight-month apparent survival rate of 0.27. In scenarios where fish can already access suitable habitat, removal of dams may not result in substantial increases in fish movement and colonisation of newly accessible habitat may not occur immediately.

Wisconsin

Shift in piscivory by salmonids following invasion of a minnow in an oligotrophic reservoir

Predation can play an important role in structuring ecological communities, and predator–prey dynamics can be altered following the introduction of new species. An unauthorized introduction of redside shiner ( Richardsonius balteatus ) into reservoirs in the Upper Skagit River, Washington, USA created concern that a consequent shift in predator–prey dynamics in the reservoirs could reduce recruitment and production of native salmonids in the basin. We estimated predation mortality in Ross Lake on nonnative redside shiner and juvenile native salmonids to evaluate the potential role of predation in regulating these populations and limiting survival of native species of concern. We used bioenergetics modelling and stable isotope analysis combined with directed field measurements of growth, seasonal diet and thermal experience of piscivorous salmonids to quantify their consumption demand on prey fishes to evaluate the relative magnitude of predation mortality on invasive redside shiners and native salmonids. While redside shiner are the dominant prey fish species in Ross Lake, the modest biomass of native salmonids consumed could translate into substantial mortality, the magnitude of which depended on the timing and size at which prey fishes were eaten. This information provides important context for how nonnative species may indirectly impact native species through shared predation (apparent competition) and can inform conservation decisions surrounding nonnative species control, sustainability of native salmonids and introductions of anadromous fishes.

Ecology of Freshwater Fish

Deposited sediment influences occurrence of functional traits of stream fishes

To better understand stream-fish sensitivity to fine sediment, we documented assemblage-wide responses by selected traits along a sedimentation gradient. We then discuss the management implications of these ‘dose–response’ relations in the contexts of biotic assessments and conservation of sediment-sensitive species. We identified a spatial gradient in sediment deposition among streams within the upper Piedmont of the Roanoke River basin in North Carolina and Virginia. We assessed fine-sediment sensitivity of 81 species based on eight species traits stratified by four attributes: food preference, feeding location, spawning substrate and spawning behaviour. We then ranked each trait and scored each species with respect to its sediment sensitivity. Using data from electrofishing surveys during 2018–2019, we calculated proportional abundances of traits observed at 30 sites throughout the study area and grouped species by their aggregate sensitivity scores. We assessed relations between embeddedness and silt cover and occurrences of species and traits using a combination of regression and ordination approaches. All traits tested responded to embeddedness or silt cover, or both. Feeding traits exhibited the strongest responses to embeddedness, while reproductive traits exhibited the strongest responses to silt cover. Our findings indicate that negative responses of the probability of presence for high-sensitivity traits to embeddedness and silt cover were linear, with no apparent thresholds. Additionally, proportional abundances of species with multiple high-sensitivity traits were inversely related to embeddedness and silt cover. Overall, our findings regarding population-level responses to sedimentation were consistent with our findings for trait-specific responses. Our analysis of species sensitivity to fine sediment corroborated the patterns we saw in our trait-specific analyses, indicating that population responses to sedimentation can be predicted from combinations of species traits. The ‘dose–response’ relations we documented may be applicable to managing sediment impacts on fishes, especially in the contexts of biotic assessments and conservation of sediment-sensitive species.

North Carolina, Virginia

Seasonal variation in habitat use by marsh fishes

We used I-m 2 throw traps to examine habitat use by smallsized fishes within a mosaic of wet prairies and sloughs in the headwaters of the St. Johns River, Florida between August 1992 and November 1995. Estimates of total fish density and biomass varied temporally, but did not differ significantly between habitats. Patterns of habitat use, however, differed among the five numerically dominant species. Bluefin killifish, mosquitofish, and golden topminnows were more abundant in sloughs than in wet prairies. In contrast, Everglades pygmy sunfish were more abundant in wet prairies than in sloughs. Finally, the abundance of least killifish did not differ between habitats. Fish densities were positively correlated with plant biomass (i. e., habitat complexity) and negatively correlated with water depth (i. e., hydrology). Species richness and composition were similar among habitats. However, consistent differences in the relative abundance of numerically dominant species between habitats indicated some degree of habitat-specific assemblage structure. Most species were concentrated into deeper sloughs during drying events. This assemblage of small-sized fishes appears to respond relatively rapidly to changes in habitat structure and hydrologic conditions. We therefore recommend that resource managers consider using fishes as indicator taxa to evaluate the efficacy of ongoing restoration and management efforts in wetland systems.

Florida

Climate-induced seasonal changes in smallmouth bass growth rate potential at the southern range extent

Temperature increases due to climate change over the coming century will likely affect smallmouth bass ( Micropterus dolomieu ) growth in lotic systems at the southern extent of their native range. However, the thermal response of a stream to warming climate conditions could be affected by the flow regime of each stream, mitigating the effects on smallmouth bass populations. We developed bioenergetics models to compare change in smallmouth bass growth rate potential (GRP) from present to future projected monthly stream temperatures across two flow regimes: runoff and groundwater-dominated. Seasonal differences in GRP between stream types were then compared. The models were developed for fourteen streams within the Ozark–Ouachita Interior Highlands in Arkansas, Oklahoma and Missouri, USA, which contain smallmouth bass. In our simulations, smallmouth bass mean GRP during summer months decreased by 0.005 g g −1 day −1 in runoff streams and 0.002 g g −1 day −1 in groundwater streams by the end of century. Mean GRP during winter, fall and early spring increased under future climate conditions within both stream types (e.g., 0.00019 g g −1 day −1 in runoff and 0.0014 g g −1 day −1 in groundwater streams in spring months). We found significant differences in change in GRP between runoff and groundwater streams in three seasons in end-of-century simulations (spring, summer and fall). Potential differences in stream temperature across flow regimes could be an important habitat component to consider when investigating effects of climate change as fishes from various flow regimes that are relatively close geographically could be affected differently by warming climate conditions.

Ecology of Freshwater Fish