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Activities and preliminary results of nearshore benthic habitat mapping in southern California, 1998

The nearshore benthic habitat of the Santa Barbara coast and Channel Islands supports a diversity of marine life that are commercially, recreationally, and intrinsically valuable. Some of these resources are known to be endangered including a variety of rockfish and the White Abalone. State and National agencies have been mandated to preserve and enhance these resources and require detailed habitat characterization in order to do so. This project will characterize and map the benthic habitat in areas that have been selected because they have been set aside as National Sanctuaries or State Preserves, or are areas of ongoing or planned fish population studies. Various management strategies are being developed to protect marine resources in the Santa Barbara Channel Islands Region. One approach under investigation is to implement no-take marine reserves (Agardy, T., 1997; Bohnsack, 1998; Roberts, 1997). One small reserve presently exists on Anacapa Island and there is a growing momentum to add additional reserves to form a reserve network (Lafferty et al., 2000). Reserves may provide relatively pristine marine communities in a wild state for study and appreciation. In addition, they may buffer some species from over-fishing. A key feature of marine reserve design is to protect a representation of the existing habitats in a region (Roberts, 1997). Unfortunately, the distribution of habitats is not well known in this area since the underwater equivalent of soils and vegetation maps that are widely available for terrestrial systems do not yet exist. Managers need habitat maps to help determine the most appropriate boundaries for reserves in a network in order to meet various criteria and goals (such as habitat representation, reserve size, habitat heterogeneity, reserve spacing, inclusion of sensitive habitats, etc.). Another use for habitat mapping is to better understand the distribution of those habitats that are particularly important to fished species or sensitive species. Combining habitat mapping with ongoing studies of egg and larval fish counts by the National Marine Fisheries Service (Russell Vetter), rockfish population studies by the California Department of Fish and Game (Dave VenTresca), and white abalone (Kevin Lafferty and others, USGS) will extend the ability to predict the distribution of these species and identify areas with appropriate habitat that might be suitable for restoration. Additional uses for habitat mapping include managing visitor use, kelp distribution, and archeological resources.

California

Survival and migration behavior of juvenile coho salmon in the Klamath River relative to discharge at Iron Gate Dam, Northern California, 2007

This report describes a study of survival and migration behavior of juvenile coho salmon in the Klamath River relative to discharge at Iron Gate Dam in 2006. This was the second year of a multi-year study with the goal of determining the effects of discharge at Iron Gate Dam on survival of juvenile coho salmon downstream. The study was a collaborative effort among U.S. Geological Survey (USGS), U.S. Fish and Wildlife Service (USFWS), and the Yurok and Karuk Tribal Fisheries Departments. The goals of the study included: 1) estimating the survival of wild and hatchery juvenile coho salmon in the Klamath River downstream from Iron Gate Dam, 2) determining the effects of discharge and other covariates on their survival and migration, and 3) determining if fish from Iron Gate Hatchery could be used as surrogates for the limited source of wild fish. The major findings of the study in 2006 include: River discharges during the 2006 study period (4 April through 21 July 2006) were among the greatest on record. Average daily discharge at Iron Gate Dam was 3,956 cubic feet per second (cfs) and ranged from 997 to 10,300 cfs. Discharge at Iron Gate Dam was positively correlated with discharges of tributaries downstream due to the above average water year and frequent occurrence of spill at Iron Gate Dam. Average daily discharge near the estuary was 25,789 cfs and ranged from 4,740 to 50,600 cfs. This study was based on hatchery fish taken directly from a tank at Iron Gate Hatchery and wild fish captured in a rotary trap on the Shasta River. Releases of both groups began on 4 April when the catch of wild fish in California Department of Fish and Game‟s Shasta River rotary trap increased, but trap catches varied throughout the study period, resulting in differences in release dates of hatchery and wild fish. A total of 211 hatchery fish were released from 4 April through 26 May. Wild and hatchery fish released on a regular schedule between 25 April and 16 May 2006 were used in comparisons of the survival and migration of hatchery (N = 120) and wild (N = 162) fish. Additional analyses were performed using hatchery fish from all dates. The data and models did not support clear differences between survivals of hatchery and wild fish released on common dates, so estimates of reach survivals were made after pooling these data. Estimates of survival were lowest in the Iron Gate Dam to Scott River reach (0.813) and greatest in the Salmon River to Trinity River reach (1.000). The overall survival from river kilometer 309 (Iron Gate Hatchery) to river kilometer 33 was 0.653 (95% CI 0.578 to 0.729). Estimates of survival based on all hatchery fish releases were similar to those from release dates common to hatchery and wild fish and are similar to those in other river systems over similar distances. The migrations of hatchery and wild fish were different in the uppermost sections of the study area and were similar thereafter. A lag between release and migration, primarily upstream from the Scott River (river kilometer 234), was present in hatchery fish to a greater extent than in wild fish, resulting in differences in migration rates. Fish from both origins spent more time between release and the Scott River than in individual reaches downstream, and this was the only reach in which travel times of fish increased as discharge decreased. The travel times of hatchery and wild fish between sites were statistically similar downstream from Indian Creek (river kilometer 178). There were differences and similarities in the analyses of the effects of covariates on survivals of hatchery and wild fish. The models of covariate effects based on hatchery and wild fish released on common dates indicated effects on wild fish survival that were not supported in data from hatchery fish. However, when the entire suite of hatchery fish releases were used the results of the analyses were similar to those based on wild fish. In both instances the effects of temperature and release date were primarily in the first reach, the reach fish of both origins spent most of their time within. The signs of the effects of these covariates differed among the fish origins (negative for wild and positive for hatchery fish), presumably due to differences in their migrations in the first reach. The effects of dam discharge on survivals of hatchery and wild fish were generally similar (positive relation), and the effects on hatchery, and to a lesser extent wild, fish were largely downstream from the Scott River. This is likely due to the prolonged residence of the naïve hatchery fish, and to a lesser extent, migrant wild fish between release and the Scott River. Inasmuch as the differences between hatchery and wild fish we observed were likely those of migrants vs. non-migrants, the use of hatchery fish captured as they are migrating downstream, rather than those directly from hatchery tanks (i.e., naïve), may improve similarities between hatchery and wild fish in future studies. The data and models used in 2006 do not support the use of naïve hatchery fish as surrogates for migrant wild fish in determining the effects of discharge on survival upstream from the Scott River. This conclusion is based on the different effects of covariates in this reach that were likely attributable to the differences in hatchery and wild migration behaviors in this reach. The results of this second year of research provide insight to the migration and survival of hatchery and wild juvenile coho salmon in the Klamath River, but the results are from a single unusual water year. The results may be different during other water year types. The current information supports a positive relation between discharge at Iron Gate Dam and survival of juvenile coho salmon downstream, but additional data should be used to refine this relation. Discharge at the dam was correlated with discharges of Klamath River tributaries during this above average water year. The data and models from the 2006 study provide the first estimates of survival of these fish in the Klamath River and can be used with data from years with other water year types to examine the effects of discharge on survival. This will only be possible over a period of years in which the correlations between discharge and other factors, such as water temperature and date, are diminished. An experimental approach in which discharges are varied at Iron Gate Dam is the most direct method to determine if survivals are affected by discharge, but this may not be feasible given the limited storage capacity of the project.

California

Final report: Baseline selenium monitoring of agricultural drains operated by the Imperial Irrigation District in the Salton Sea Basin

This report summarizes comprehensive findings from a 4-year-long field investigation to document baseline environmental conditions in 29 agricultural drains and ponds operated by the Imperial Irrigation District along the southern border of the Salton Sea. Routine water-quality collections and fish community assessments were conducted on as many as 16 sampling dates at roughly quarterly intervals from July 2005 to April 2009. The water-quality measurements included total suspended solids and total (particulate plus dissolved) selenium. With one exception, fish were surveyed with baited minnow traps at quarterly intervals during the same time period. However, in July 2007, fish surveys were not conducted because we lacked permission from the California Department of Fish and Game for incidental take of desert pupfish ( Cyprinodon macularius ), an endangered species. During April and October 2006–08, water samples also were collected from seven intensively monitored drains (which were selected from the 29 total drains) for measurement of particulate and dissolved selenium, including inorganic and organic fractions. In addition, sediment, aquatic food chain matrices [particulate organic detritus, filamentous algae, net plankton, and midge (chironomid) larvae], and two fish species (western mosquitofish, Gambusia affinis ; and sailfin molly, Poecilia latipinna ) were sampled from the seven drains for measurement of total selenium concentrations. The mosquitofish and mollies were intended to serve as surrogates for pupfish, which we were not permitted to sacrifice for selenium determinations. Water quality (temperature, dissolved oxygen, pH, specific conductance, and turbidity) values were typical of surface waters in a hot, arid climate. A few drains exhibited brackish, near-anoxic conditions, especially during summer and fall when water temperatures occasionally exceeded 30 degrees Celsius. Total selenium concentrations in water were directly correlated with salinity and inversely correlated with total suspended-solids concentrations. Although pupfish were found in several drains, sometimes in relatively high numbers, the fish faunas of most drains and ponds were dominated by nonnative species, especially mosquitofish, mollies, and red shiner ( Cyprinella lutrensis ). Dissolved selenium in water samples from the seven intensively monitored drains ranged from 0.700 to 32.8 micrograms per liter (?g/L), with selenate as the major constituent. Selenium concentrations in other matrices varied widely among drains and ponds, with one drain (Trifolium 18) exhibiting especially high concentrations in food chain matrices [particulate organic detritus, 5.98–58.0 micrograms of selenium per gram (?g Se/g); midge larvae, 12.7–50.6 ?g Se/g] and in fish (mosquitofish, 13.2–20.2 ?g Se/g; sailfin mollies, 12.8–30.4 ?g Se/g; all concentrations are based on dry weights). Although selenium was accumulated by all trophic levels, biomagnification (defined as a progressive increase in selenium concentration from one trophic level to the next higher level) in midge larvae and fish occurred only at lower exposure concentrations. Judging mostly from circumstantial evidence, the health and wellbeing of poeciliids and pupfish are not believed to be threatened by ambient exposure to selenium in the drains and ponds.

California

A conceptual model for site-level ecology of the giant gartersnake ( Thamnophis gigas ) in the Sacramento Valley, California

Giant gartersnakes (Thamnophis gigas) comprise a species of semi-aquatic snakes precinctive to marshes in the Central Valley of California (Hansen and Brode, 1980; Rossman and others, 1996). Because more than 90 percent of their historical wetland habitat has been converted to other uses (Frayer and others, 1989; Garone, 2007), giant gartersnakes have been listed as threatened by the State of California (California Department of Fish and Game Commission , 1971) and the United States (U.S. Fish and Wildlife Service, 1993). Giant gartersnakes currently occur in a highly modified landscape, with most extant populations occurring in the rice - growing regions of the Sacramento Valley, especially near areas that historically were tule marsh habitat (Halstead and others, 2010, 2014). In ricelands and managed marshes, many operational decisions likely affect the health and viability of giant gartersnake populations. Land-use decisions, including the management of water, aquatic vegetation, terrestrial vegetation, and co-occurring species, have the potential to affect giant gartersnakes. Little is known, however, about the effects of these types of decisions on the viability of giant gartersnake populations. Bayesian network models are a useful tool to help guide decisions with uncertain outcomes. These models require the articulation of what experts think they know about a system, and facilitate learning about the hypothesized relations (Marcot and others, 2001; Uusitalo , 2007). Bayesian networks further provide a clear visual display of the model that facilitates understanding among various stakeholders (Marcot and others, 2001; Uusitalo , 2007). Empirical data and expert judgment can be combined, as continuous or categorical variables, to update knowledge about the system (Marcot and others, 2001; Uusitalo , 2007). Importantly, Bayesian network models allow inference from causes to consequences, but also from consequences to causes, so that data can inform the states of nodes (values of different random variables) in either direction (Marcot and others, 2001; Uusitalo , 2007). Because they can incorporate both decision nodes that represent management actions and utility nodes that quantify the costs and benefits of outcomes, Bayesian networks are ideally suited to risk analysis and adaptive management (Nyberg and others, 2006; Howes and others, 2010). Thus, Bayesian network models are useful in situations where empirical data are not available, such as questions concerning the responses of giant gartersnakes to management.

California

Desert tortoise annotated bibliography, 1991-2015

Introduction Agassiz’s desert tortoise, Gopherus agassizii , was considered a single species for 150 years after its discovery by James Cooper (1861), with a geographic range extending from southeastern California, southern Nevada, and southwestern Utah southward into northern Sinaloa, Mexico (Murphy and others, 2011). What was once G. agassizii is now recognized as a complex composed of three sister species, G. agassizii , G. morafkai , and G. evgoodei (Murphy and others, 2011; Edwards and others, 2016) (fig. 1). The geographic range of Agassiz’s Desert Tortoise ( G. agassizii ) is now limited to north and west of the Colorado River (Murphy and others, 2011), with the exception of a small population in northwestern Arizona (Edwards and others, 2015). This annotated bibliography is based on peer-reviewed journal articles published between January 1991 and December 2015 on Agassiz’s Desert Tortoise, with the geographic range as defined by Murphy and others (2011). Studies pertaining to other species of Gopherus (e.g., G. morafkai ), were included only when associated with G. agassizii . In addition to articles pertaining directly to desert tortoises, we compiled articles concerning threats to desert tortoises and the habitats they occupy. Similarly, we only included studies that encompass other habitat types when they were directly compared with habitats of G. agassizii . Agassiz’s Desert Tortoise (hereinafter called desert tortoise) is a state- and federally-listed threatened species (U.S. Fish and Wildlife Service, 1990; California Department of Fish and Game, 2015). The first population federally listed as threatened occurred on the Beaver Dam Slope, Utah (U.S. Fish and Wildlife Service, 1980). In 1990, the entire geographic range north and west of the Colorado River was federally listed as threatened (U.S. Fish and Wildlife Service, 1990), with the exception being a small population in northwestern Arizona. The purpose of this annotated bibliography is to support recovery efforts for the species, because populations have continued to decline in spite of designation of critical habitat and publication of a recovery plan (U.S. Fish and Wildlife Service, 1994). For example, between 2005 and 2014, populations in critical habitats declined about 50% (U.S. Fish and Wildlife Service, 2015).

Open-File Report

Mortality monitoring design for utility-scale solar power facilities

Introduction Solar power represents an important and rapidly expanding component of the renewable energy portfolio of the United States (Lovich and Ennen, 2011; Hernandez and others, 2014). Understanding the impacts of renewable energy development on wildlife is a priority for the U.S. Fish and Wildlife Service (FWS) in compliance with Department of Interior Order No. 3285 (U.S. Department of the Interior, 2009) to “develop best management practices for renewable energy and transmission projects on the public lands to ensure the most environmentally responsible development and delivery of renewable energy.” Recent studies examining effects of renewable energy development on mortality of migratory birds have primarily focused on wind energy (California Energy Commission and California Department of Fish and Game, 2007), and in 2012 the FWS published guidance for addressing wildlife conservation concerns at all stages of land-based wind energy development (U.S. Fish and Wildlife Service, 2012). As yet, no similar guidelines exist for solar development, and no published studies have directly addressed the methodology needed to accurately estimate mortality of birds and bats at solar facilities. In the absence of such guidelines, ad hoc methodologies applied to solar energy projects may lead to estimates of wildlife mortality rates that are insufficiently accurate and precise to meaningfully inform conversations regarding unintended consequences of this energy source and management decisions to mitigate impacts. Although significant advances in monitoring protocols for wind facilities have been made in recent years, there remains a need to provide consistent guidance and study design to quantify mortality of bats, and resident and migrating birds at solar power facilities (Walston and others, 2015). In this document, we suggest methods for mortality monitoring at solar facilities that are based on current methods used at wind power facilities but adapted for the unique conditions encountered at solar facilities. In particular, unlike at wind-power facilities, the unimpeded access to almost all areas within the facilities, the typically flat terrain, and general absence of thick vegetation allow distance-sampling techniques (Buckland and others, 2001, 2004) to be exploited to advantage at industrial solar sites. These protocols build on the work of Nicolai and others (2011), and as our understanding and techniques for monitoring improve, the methods may be further modified to incorporate improvements in the future. We present case studies based on monitoring methods currently implemented at different utility-scale solar facilities to illustrate how distance-sampling techniques may improve overall detectability without substantially increasing costs. Every facility is unique, and the protocols presented may be adapted based on specific monitoring objectives and conditions at each site. We provide guidance for designing monitoring programs whose objective it is to estimate the total number of bird and bat fatalities occurring at a facility over an extended period of time. We address spatial variation in causes of mortality, as well as potential sources of imperfect detection, for example, animals falling in or moving to unsearched areas, carcasses removed by predators, and carcasses missed by searchers. We suggest methods to estimate and account for each source of imperfect detection. This document focuses on monitoring design only and does not discuss approaches for estimating mortality from collected data. The development of statistically sound estimators relevant to the solar context is a current topic of research, although there are already strong foundations for estimation with distance-sampling methods in similar open, arid environments (Anderson and others, 2001; Freilich and others, 2005). Nonetheless, if protocols described in this document are followed, the resulting data will be adequate and sufficient for estimating mortality using newly formulated estimators.

Open-File Report

Klamath River Basin Hydrologic Conditions Prior to the September 2002 Die-Off of Salmon and Steelhead

More than 33,000 salmon and steelhead died in the lower Klamath River in late September 2002 on their way to spawning areas upstream. According to the California Department of Fish and Game, the cause of death was infection by protozoan and bacterial pathogens. Two factors that may have contributed to the disease incidence are low streamflow and high water temperature. September streamflows throughout the Klamath Basin were low, among the four lowest September flows recorded on the main stem since 1960. The low streamflows were caused by below-average snowpack and long-term drought, with resulting reduced ground-water discharge to streams. On the basis of historical climate data from the Klamath Basin and historical water temperature data from an adjacent basin, September 2002 water temperatures were above the long-term average. Temperatures in the Klamath River above the fish die-off reach exceeded 65 degrees Fahrenheit for nearly all of September; multiple days of exposure by fish to temperatures at or above that level can greatly increase disease incidence. This report characterizes streamflow and water temperature conditions during the period leading up to the die-off and compares them to historical conditions in the Klamath River. This report is not an exploration of the causative mechanism of the die-off; rather, it is intended to provide detailed documentation of these conditions to be used by those examining the cause(s) of the die-off and to provide information that can contribute to decisions about future water management in the Klamath Basin.

Water-Resources Investigations Report

Mechanical suppression of northern pike (Esox lucius) populations in small Arizona reservoirs

Introduced populations of northern pike Esox lucius have provided angling opportunities in the western United States (McMahon and Bennett 1996). However, the northern pike is a voracious piscivore and its large size, high fecundity, and broad physiological tolerance make it capable of drastically altering ecosystems it invades (Marchetti et al. 2004). Indeed, predation by northern pike has been shown to significantly alter fish community structure and put native fishes at a higher extinction risk (He and Kitchell 1990, Findlay et al. 2000). Predation by northern pike is viewed as a significant threat to native stocks of salmonids in Washington, British Columbia, and California (McMahon and Bennett 1996, California Department of Fish and Game [CDFG] 2003).

Arizona

Potential exposure of larval and juvenile delta smelt to dissolved pesticides in the Sacramento-San Joaquin Delta, California

The San Francisco Estuary is critical habitat for delta smelt Hypomesus transpacificus, a fish whose abundance has declined greatly since 1983 and is now listed as threatened. In addition, the estuary receives drainage from the Central Valley, an urban and agricultural region with intense and diverse pesticide usage. One possible factor of the delta smelt population decline is pesticide toxicity during vulnerable larval and juvenile stages, but pesticide concentrations are not well characterized in delta smelt spawning and nursery habitat. The objective of this study was to estimate the potential exposure of delta smelt during their early life stages to dissolved pesticides. For 3 years (1998-2000), water samples from the Sacramento-San Joaquin Delta were collected during April-June in coordination with the California Department of Fish and Game's delta smelt early life stage monitoring program. Samples were analyzed for pesticides using solid-phase extraction and gas chromatography/mass spectrometry. Water samples contained multiple pesticides, ranging from 2 to 14 pesticides in each sample. In both 1999 and 2000, elevated concentrations of pesticides overlapped in time and space with peak densities of larval and juvenile delta smelt. In contrast, high spring outflows in 1998 transported delta smelt away from the pesticide sampling sites so that exposure could not be estimated. During 2 years, larval and juvenile delta smelt were potentially exposed to a complex mixture of pesticides for a minimum of 2-3 weeks. Although the measured concentrations were well below short-term (96-h) LC50 values for individual pesticides, the combination of multiple pesticides and lengthy exposure duration could potentially have lethal or sublethal effects on delta smelt, especially during early larval development.

American Fisheries Society Symposium

The ecology of the soft-bottom benthos of San Francisco Bay: a community profile

This profile, part of a series of profiles concerning coastal habitats of the United States, is a detailed examination of the soft-bottom benthos of San Francisco Bay. A U.S. Fish and Wildlife Service and California Department of Fish and Game report (1979) entitled "Protection and Restoration of San Francisco Bay Fish and Wildlife Habitat" provides clear recognition of the importance of intertidal and subtidal soft-bottom habitats and their associated organisms to the bay's birds and fishes and to the overall functioning of the estuary. The purpose of this profile is to provide a description of the structure and functioning of the benthic community in San Francisco Bay (exclusive of its tidal marshes, which are discussed by M. Josselyn [1983] in another profile). The habitats covered in this volume include all nonvegetated soft-bottom intertidal and subtidal areas of the bay between the Golden Gate and the mouths of the Sacramento and San Joaquin Rivers to the northeast, and to the southern extremity of the bay. The profile provides a reference to the scientific information concerning the animals and plants of the bay's benthic communities, their importance to the bay ecosystem, and their value as a resource measured in human terms. Because there have been few process-oriented studies of the benthos of San Francisco Bay (e.g., field and laboratory rate-measurement experiments), the material presented herein is largely descriptive. Nonetheless, we have described the processes that interconnect the various physical, chemical, and biological components of the benthic environment, and the important couplings between this environment and the water column above, with reference to research results from other estuaries where necessary. We consider the role of the benthic community as a food source for fish, aquatic birds, and humans; as a consumer or degrader of organic materials including wastes; as a recycler of minerals and nutrients; and as an accumulator of pollutants.

California

Re-introduction of tule elk to Point Reyes National Seashore, California, USA

Tule elk (Cervus elaphus nannodes), a subspecies endemic to California, was historically found in large herds throughout much of central and coastal California. Market hunting during the California Gold Rush decimated these herds, and by 1895, only two to 10 elk remained. This remnant group was protected and served as the source for early relocation efforts (McCullough, 1971). Early efforts were generally unsuccessful but did establish a herd in California’s Owens Valley, outside their historical range, in 1933. The herd grew rapidly and supported six controversial hunts between 1943 and 1969. In an effort to limit hunting, concerned preservationists formed the Committee for the Preservation of Tule Elk in 1960. Public pressure resulted in the California State Legislature passing a law in 1971 that halted hunting until either state-wide numbers reached 2,000, or no further unoccupied elk habitat existed. This law prompted the California Department of Fish and Game to begin reintroducing tule elk throughout their former range. In 1976, the U.S. Congress passed a resolution that concurred with state law and directed federal agencies to make lands available for reintroductions within the subspecies’ historical range. Point Reyes National Seashore was identified as a potential translocation site.

California

Ecotypic variation in recruitment of reintroduced bighorn sheep: implications for translocation

European settlement led to extirpation of native Audubon's bighorn sheep (formerly Ovis canadensis auduboni ) from North Dakota during the early 20th century. The North Dakota Game and Fish Department subsequently introduced California bighorn sheep (formerly O. c. californiana ) that were indigenous to the Williams Lake region of British Columbia, Canada, and Rocky Mountain bighorn sheep ( O. c. canadensis ) that were indigenous to the Sun River region of Montana. Although California bighorn sheep are no longer recognized as a distinct subspecies, they are smaller and adapted to a milder climate than either the native bighorn sheep of North Dakota or introduced bighorn sheep from Montana. Because reintroductions still play a key role in the management of bighorn sheep and because local adaptation may have substantial demographic consequences, we evaluated causes of variation in recruitment of bighorn sheep reintroduced in North Dakota. During 2006–2011, Montana stock recruited 0.54 juveniles/adult female (n = 113), whereas British Columbia stock recruited 0.24 juveniles/adult female (n = 562). Our most plausible mixed-effects logistic regression model (53% of model weight) attributed variation in recruitment to differences between source populations (odds ratio = 4.5; 90% CI = 1.5, 15.3). Greater recruitment of Montana stock (fitted mean = 0.56 juveniles/adult female; 90% CI = 0.41, 0.70) contributed to a net gain in abundance (r = 0.15), whereas abundance of British Columbia stock declined (fitted mean = 0.24 juveniles/adult female; 90% CI = 0.09, 0.41; r = − 0.04). Translocations have been the primary tool used to augment and restore populations of wild sheep but often have failed to achieve objectives. Our results show that ecotypic differences among source stocks may have long-term implications for recruitment and demographic performance of reintroduced populations.

North Dakota

Policy comparison of lead hunting ammunition bans and voluntary nonlead programs for California condors

The endangered California condor ( Gymnogyps californianus ) is negatively affected by lead poisoning from spent lead-based hunting ammunition. Because lead poisoning is the primary mortality factor affecting condors, the California Fish and Game Commission banned lead hunting ammunition during 2008 in the southern California condor range followed by a statewide ban implemented in 2019. In contrast, the Arizona Game and Fish Department instituted an outreach and awareness program encouraging voluntary use of nonlead hunting ammunition in the northern portion of the state during 2005 and a similar program was launched in Utah during 2012. The juxtaposition of policy tools provided a unique opportunity to evaluate the intended efforts to mitigate lead exposure in condors and their respective positive and negative effects. Herein we reflect upon the effectiveness of lead policy actions in the 3-state region on the basis of condor blood-lead levels, population status, and hunter awareness of the issue and use of nonlead hunting ammunition.

California

Forestry practices and aquatic biodiversity: Fish

In the Pacific Northwest, fish communities are found in a diverse array of aquatic habitats ranging from the large coastal rivers of the temperate rainforests, to the fragmented and sometimes ephemeral streams of the xeric interior basins, and high-elevation streams and lakes in the mountainous areas (Rieman et al. 2003). Only high-elevation lakes and streams isolated above barriers to fish passage remained historically devoid of fish because they were never invaded following Pleistocene glaciation (Smith 1981). Despite this widespread distribution and once great population abundances, taxonomic diversity of fishes in these forested systems is naturally lower than in aquatic habitats in the eastern U.S. (Reeves, Bisson, and Dambacher 1998). Interactions among factors that influence species richness in aquatic systems (e.g., basin size, long-term stability of habitat, and barriers to colonization; Smith 1981) continue to influence the occurrence and persistence of fishes in these systems today. Consequently, the larger low-elevation rivers and estuaries support the greatest variety of fish species. In the high-elevation tributary streams, fish communities are less complex because these aquatic systems were less climatically and geologically stable, and fish populations were smaller and more prone to local extirpation. Furthermore, barriers to fish passage inhibited dispersal and colonization (Smith 1981). Streams in forested landscapes generally support salmon and trout, Oncorhynchus spp., whitefish Prosopium spp., sculpins Cottus spp., suckers Catostomus spp., and minnows (Cyprinidae), but in some of the colder streams, chars (e.g., Salvelinus confluentus and Salvelinus malma ) and lampreys (Petromyzontidae)may also occur (Rieman et al. 2003). Although biodiversity defined in terms of fish species richness is low in the Pacific Northwest, intraspecific variability is high, and polytypic fish species are common in the diverse aquatic habitats of the region. For example, the salmonids in the coastal rivers and streams, and the larger interconnected streams, rivers, and lakes of the interior exhibit a variety of ecotypes and migratory life histories (Healey 1986; Trotter 1989; Larson and McIntire 1993; Northcote 1997). This life-history variation appears to be associated with adaptation to spatial and temporal variation in environment (e.g., Schaffer and Elson 1975; Carl and Healey 1984; Beacham and Murray 1987), and there is some evidence of the genetic heritability of life-history traits (Carl and Healey 1984; Gharrett and Smoker 1993; Hankin, Nicholas, and Downey 1993). Persistence of any level of biological organization (e.g., life-history type, population, metapopulation, subspecies, species, community) is related to the interaction of environmental and biological components, and intraspecific diversity is a means of spreading risk ( sensu den Boer 1968) of extirpation in dynamic environments (Gresswell 1999). Unfortunately, despite the broad distribution and extensive intraspecific diversity, persistence of native fishes is uncertain in the Pacific Northwest. Many populations of anadromous salmonids, once synonymous with vigorous biological communities throughout the region, are threatened with extinction (Nehlsen, Williams, and Lichatowich 1991; Frissell 1993; Thurow, Lee, and Rieman 1997). Furthermore, over half of the native taxa in the Columbia River Basin are either listed under the Endangered Species Act, are being considered for listing, or are deemed sensitive by the management agencies (Lee et al. 1997; Thurow, Lee, and Rieman 1997). Potamodromous species like bull trout Salvelinus confluentus are estimated to occur as strong populations in less than 5% of their potential range (Rieman, Lee, and Thurow 1997). Although not currently listed under the endangered species list, the coastal cutthroat trout Oncorhynchus clarki is managed as a sensitive species in Oregon and California (Hall, Bisson, and Gresswell 1997). Native non-game fishes have rarely been monitored, but populations of species such as large-scale suckers ( Catostomus macrocheilus ), squawfish ( Ptychocheilus umpquae ), and Pacific lamprey ( Lampetra tridentata ) also are declining in some drainages (Oregon Department of Fish and Wildlife, unpublished data).

Pacific Northwest

Translocated sea otter populations off the coasts of Oregon and Washington

The historical distribution of sea otters extended from the northern islands of Japan north and east across the Aleutian chain to the mainland of North America then south along the west coast to central Baja California, Mexico (Riedman and Estes 1990). By the beginning of the twentieth century, after 150 years of being intensively hunted for their valuable fur, sea otters had been extirpated from most of their range (Kenyon 1969). In 1911 sea otters were protected by the passage of the International Fur Seal Treaty. Unfortunately, only 13 remnant populations survived the fur-hunting period, and two of those, British Columbia and Mexico, would also ultimately disappear, leaving only a small group of sea otters south of Alaska, along the rugged Big Sur coast of California (Kenyon 1969). The earliest attempts to reestablish sea otters to unoccupied habitat were begun in the early 1950’s by R. D. (Sea Otter) Jones, then manager of the Aleutian National Wildlife Refuge (Kenyon 1969). These early efforts were experimental, and all failed to establish populations. However, the knowledge gained from Jones’s efforts and the seminal work of Kenyon (1969) and others during the 1950’s and early 1960’s ultimately led to the successful efforts to come. During the mid-1960’s the Alaska Department of Fish and Game began translocating sea otters to sites where the species had occurred before the fur-trade period. The first translocations were restricted to Alaska, but beginning in 1969 and continuing through 1972, the effort expanded beyond Alaska. During this period, 241 sea otters were translocated to sites in British Columbia, Washington, and Oregon (Jameson et al. 1982). The work was done cooperatively between state and provincial conservation agencies, with much of the financial support for the Oregon and Washington efforts coming from the Atomic Energy Commission (now ERDA). Followup studies of the Oregon population began in 1971 and continued through 1975. After 1975, surveys in Oregon occurred infrequently. In Washington no follow-up surveys were conducted until 1977, although the population has been monitored closely since then (Jameson et al. 1982, 1986; Jeffries and Jameson 1995).

Oregon, Washington

North American Bat Monitoring Program regional protocol for surveying with stationary deployments of echolocation recording devices: Narrative version 1.0, Pacific Northwestern US

The outbreak of white-nose syndrome (WNS) and the growing awareness of the risks to bats from wind power generating facilities have driven radical changes to North American bat conservation. Over the last decade, formerly common species such as the little brown myotis (Myotis lucifugus) and hoary bat (Lasiurus cinereus) have experienced unprecedented mortality rates and are now facing non-trivial extinction risk. In response to this change, federal land management agencies such as the US National Park Service, US Fish and Wildlife Service, US Forest Service, US Bureau of Land Management and state wildlife management agencies such as the Oregon Department of Fish and Wildlife and Idaho Fish and Game have invested in collaborative, interagency bat monitoring to close the gap in information about bat welfare and to inform bat conservation strategies. Bats are notoriously difficult to track and study and there remains a paucity of fundamental information about the seasonal patterns of bat activity and habitat use and population distributions and abundances. Moreover, because bats are so highly mobile and difficult to survey (e.g., nocturnal flight), this information needs to be contextualized at broad regional (e.g., 10,000 km2) and range-wide extents. Delimiting bat populations at local scales (e.g., 100 km2) is very difficult and it is not clear, for example, how a declining trend in local (e.g., a small park unit) patterns of bat activity or relative abundance should be interpreted without broader context. In recognition of these challenges, a plan for coordinated continental-scale monitoring of bats, the North American Bat Monitoring Program (NABat) was developed (Loeb et al. 2015). The centerpiece of the plan is the use of a spatially-balanced randomized master sample of grid-cell sample units from a grid-based sampling frame to provide the architecture for collaboration and the statistical foundation for making inferences about bat populations across broad regions and entire bat geographic ranges. The plan outlines general goals, survey design, and field methods for both summertime acoustic surveys of bats as well as winter and summer counts of bats in hibernacula and maternity colonies but it does not provide field-level protocol and standard operating procedures for consistent and efficient implementation. This regional protocol provides these details for one component of NABat, the deployment of stationary acoustic detectors to record bats during summer, as is called for by the NABat plan. This protocol was written specifically to provide guidance and consistency across the Pacific Northwestern US (N. California [California Department of Fish and Wildlife Northern Region], Idaho, Washington, and Oregon; US Fish and Wildlife Service Region 1 and portion of Region 8 [in Northern California and Klamath Basin]; US Forest Service Region 6 and portions of Regions 1 and 5 in Idaho; and the Upper Columbia Basin, North Coast Cascades, and Klamath Networks of the National Park Service). This region has internal cohesion, sharing a distinct bat faunal assemblage of 15 species (with several additional species occurring on the southern periphery of the region), and a long history of collaborative bat monitoring beginning with the interagency Bat Grid Program which operated from 2003-2010 across Oregon and Washington (US Forest Service Region 6). This protocol will be coordinated and implemented by the Northwestern Bat Hub, on behalf of the collective interagency partnership. The Northwestern Bat Hub is housed on the Oregon State University-Cascades campus and leverages pooled partner funds and resources to maintain a small staff that coordinates and conducts monitoring, provides training and oversight, ensures high-quality data quality and control, and analyzes data and reports on results.

California, Idaho, Oregon, Washington

Observations of the spawning ecology of the imperiled Clear Lake Hitch Lavinia exilicauda chi

Migrations for the purposes of reproduction are widely documented across the animal kingdom and are particularly common in fishes and other aquatic organisms (Dingle 2014). One important migration strategy in fishes is potamodromy, which is the movement from one location to another entirely within freshwater (Morais and Daverat 2016). Thurow (2016) estimated that worldwide there are approximately 13,000 potamodromous fish species. Potamodromous species as a group are also relatively imperiled, owing to the loss or destruction of the diversity of habitats often required for successful reproduction and recruitment (Thurow 2016). The Clear Lake Hitch Lavinia exilicauda chi is an imperiled potamodromous cyprinid that is endemic to a single freshwater lake: Clear Lake, Lake County, California, USA. The species lives to approximately six years of age and attains a maximum size of approximately 350 mm fork length. As juveniles and adults, it feeds primarily on macroinvertebrates, including insects and zooplankton (Geary and Moyle 1980). Formerly highly abundant and a staple food for the Pomo tribes of the Clear Lake region, Clear Lake Hitch abundance is believed to have declined substantially from historical levels (California Department of Fish and Wildlife [CDFW] 2014). Presently, Clear Lake Hitch is listed as threatened under the California Endangered Species Act and has been petitioned for listing under the U.S. Endangered Species Act. The purpose of this paper is to document fortuitous observations of Clear Lake Hitch spawning and holding in stream habitat to generate baseline information that is needed to manage the species. The observations facilitated addressing the following questions (1) under what water temperature and flow conditions does spawning occur?, (2) what are the major habitat features where spawning takes place?, (3) what are the fundamental aspects of spawning behavior?, (4) what is the immediate fate of eggs deposited during spawning?, and (5) when not engaged in spawning, what type of stream habitat is used by Clear Lake Hitch and do they actively feed?

California