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USGS2013

Effects of depletion sampling by standard three-pass pulsed DC electrofishing on blood chemistry parameters of fishes from Appalachian streams

Adverse effects on fishes captured by electrofishing techniques have long been recognized, although the extent of associated physical injury and behavioral alterations are highly variable and dependent on a number of factors. We examined the effects of three-pass pulsed DC (PDC) electrofishing on two salmonid species (Rainbow Trout Oncorhynchus mykiss and Brook Trout Salvelinus fontinalis) and five other genera (Green Sunfish Lepomis cyanellus, Potomac Sculpin Cottus girardi, Fathead Minnow Pimephales promelas, Largemouth Bass Micropterus salmoides, and Channel Catfish Ictalurus punctatus) common to Appalachian streams. We examined the corresponding effects of PDC electroshock on the following physiological indicators of stress and trauma: blood glucose and serum lactate, as well as on other blood chemistry, namely, enzymes, electrolytes, minerals, and proteins. All species demonstrated physiological responses to PDC electroshock, indicated by the biochemical differences in blood parameters in unshocked and shocked groups of fish with or without gross evidence of hemorrhagic trauma. Serum lactate was the most consistent indicator of these effects. Significant differences in whole blood glucose levels were also noted in treatment groups in all species except Green Sunfish, although the patterns observed were not as consistent as for serum lactate. Elevations in the serum enzymes, aspartate aminotransferase and creatine kinase, in the electroshocked fish occurred only in the two salmonid species. In many instances, although blood parameters were elevated in electroshocked fish compared with the unshocked controls for a given species, there were no differences in those levels in electroshocked fish based on the presence of gross hemorrhagic trauma to axial musculature. While some of the blood parameters examined correlated with both the occurrence of electroshock and the resultant tissue injury, there was no apparent link between the altered blood chemistry and increased mortality 30 d after electrofishing.

Appalachian Mountains↗

USGS1989

Comparison of whole body and tissue blood volumes in rainbow trout (Salmo gairdneri) with 125 I bovine serum albumin and 51 Cr-erythrocyte tracers

Total, packed cell and, plasma volume estimates were made for the whole body and selected tissues of rainbow trout by the simultaneous injection of radiolabelled trout erythrocyte ( 51 Cr-RBC) and radioiodinated bovine serum albumin ( 125 I-BSA) tracers. Blood volumes were estimated with both markers separately by the tracer-hematocrit method and as the combination of the 51 Cr-RBC packed cell and 125 I-BSA plasma volumes. Mean whole body blood volume was significantly less when calculated from the 51 Cr-RBC tracer data (3.52±0.78 ml/100 g; ±SD) than when calculated with the 125 I-BSA tracer (5.06±0.86 ml/100 g) or as the sum of the two volumes combined (4.49±0.60 ml/100 g). The whole body hematocrit (28±5%), estimated as the quotient of the 51 Cr-RBC volume divided by the sum of the 125 I-BSA and the 51 Cr-RBC volumes, also was significantly less than the dorsal aortic microhematocrit (36±4%). Estimates of total blood volumes in most tissues were significantly smaller when calculated from the 51 Cr-RBC data than when calculated by the other two methods. Tissue blood volumes were greatest in highly vascularized and well perfused tissues and least in poorly vascularized tissues. The relative degree of vascularization among tissues generally remained the same regardless of whether the red cell or the plasma tracer was used to calculated blood volume. It is not clear whether the expanded plasma volume is the result of the distribution of erythrocyte-poor blood into the secondary circulation or the result of extravascular exchange of plasma proteins.

Fish Physiology and Biochemistry↗

USGS2005

Concentrations of cadmium, lead, and zinc in fish from mining-influenced waters of northeastern Oklahoma: Sampling of blood, carcass, and liver for aquatic biomonitoring

The Tri-States Mining District (TSMD) of Missouri (MO), Kansas (KS), and Oklahoma (OK), USA, was mined for lead (Pb) and zinc (Zn) for more than a century. Mining ceased more than 30 years ago, but wastes remain widely distributed in the region, and there is evidence of surface- and groundwater contamination in the Spring River-Neosho River (SR-NR) system of northeastern OK. In October 2001, we collected a total of 74 fish from six locations in the SR-NR system that included common carp (Cyprinus carpio), channel- and flathead catfish (Ictalurus punctatus and Pylodictis olivaris), largemouth- and spotted bass (Micropterus salmoides and Micropterus punctulatus), and white crappie (Pomoxis annularis). We obtained additional fish from locations in MO that included three reference sites and one site that served as a "positive control" (heavily contaminated by Pb). Blood, carcass (headed, eviscerated, and scaled) and liver (carp only) samples were analyzed for cadmium (Cd), Pb, and Zn. Our objectives were to assess the degree to which fish from the OK portion of the SR-NR system are contaminated by these elements and to evaluate fish blood sampling for biomonitoring. Concentrations of Cd and Pb in carp and catfish from OK sites were elevated and Pb concentrations of some approached those of the highly contaminated site in MO, but concentrations in bass and crappie were relatively low. For Zn, correlations were weak among concentrations in the three tissues and none of the samples appeared to reflect site contamination. Variability was high for Cd in all three tissues of carp; differences between sites were statistically significant (p < 0.05) only for blood even though mean liver concentrations were at least 100-fold greater than those in blood. Blood concentrations of Cd and Pb were positively correlated (r 2 = 0.49 to 0.84) with the concentration of the same element in carp and catfish carcasses or in carp livers, and the corresponding multiple regression models were highly significant (p < 0.001). Our data indicate that potentially nonlethal blood sampling can be useful for monitoring of selected metals in carp, catfish, and perhaps other fishes. ?? 2005 Springer Science+Business Media, Inc.

Archives of Environmental Contamination and Toxico↗

USGS2009

Concentrations of cadmium, Cobalt, Lead, Nickel, and Zinc in Blood and Fillets of Northern Hog Sucker (Hypentelium nigricans) from streams contaminated by lead-Zinc mining: Implications for monitoring

Lead (Pb) and other metals can accumulate in northern hog sucker (Hypentelium nigricans) and other suckers (Catostomidae), which are harvested in large numbers from Ozark streams by recreational fishers. Suckers are also important in the diets of piscivorous wildlife and fishes. Suckers from streams contaminated by historic Pb-zinc (Zn) mining in southeastern Missouri are presently identified in a consumption advisory because of Pb concentrations. We evaluated blood sampling as a potentially nonlethal alternative to fillet sampling for Pb and other metals in northern hog sucker. Scaled, skin-on, bone-in "fillet" and blood samples were obtained from northern hog suckers (n = 75) collected at nine sites representing a wide range of conditions relative to Pb-Zn mining in southeastern Missouri. All samples were analyzed for cadmium (Cd), cobalt (Co), Pb, nickel (Ni), and Zn. Fillets were also analyzed for calcium as an indicator of the amount of bone, skin, and mucus included in the samples. Pb, Cd, Co, and Ni concentrations were typically higher in blood than in fillets, but Zn concentrations were similar in both sample types. Concentrations of all metals except Zn were typically higher at sites located downstream from active and historic Pb-Zn mines and related facilities than at nonmining sites. Blood concentrations of Pb, Cd, and Co were highly correlated with corresponding fillet concentrations; log-log linear regressions between concentrations in the two sample types explained 94% of the variation for Pb, 73-83% of the variation for Co, and 61% of the variation for Cd. In contrast, relations for Ni and Zn explained <12% of the total variation. Fillet Pb and calcium concentrations were correlated (r = 0.83), but only in the 12 fish from the most contaminated site; concentrations were not significantly correlated across all sites. Conversely, fillet Cd and calcium were correlated across the range of sites (r = 0.78), and the inclusion of calcium in the fillet-to-blood relation explained an additional 12% of the total variation in fillet Cd. Collectively, the results indicate that blood sampling could provide reasonably accurate and precise estimates of fillet Pb, Co, and Cd concentrations that would be suitable for identifying contaminated sites and for monitoring, but some fillet sampling might be necessary at contaminated sites for establishing consumption advisories. ?? 2009 US Government.

Archives of Environmental Contamination and Toxico↗

USGS2016

Mercury correlations among blood, muscle, and hair of northern elephant seals during the breeding and molting fasts

Mercury (Hg) biomonitoring and toxicological risk assessments for marine mammals commonly sample different tissues, making comparisons to toxicity benchmarks and among species and regions difficult. Few studies have examined how life history events, such as fasting, influence the relationship between total Hg (THg) concentrations in different tissues. We evaluated the relationships between THg concentrations in blood, muscle, and hair of female and male northern elephant seals ( Mirounga angustirostris ) at the start and end of the breeding and molting fasts. The relationships between tissues varied among tissue pairs and differed by sampling period and sex. Blood and muscle were generally related at all time periods; however, hair, an inert tissue, did not strongly represent the metabolically active tissues (blood and muscle) at all times of year. The strongest relationships between THg concentrations in hair and those in blood or muscle were observed during periods of active hair growth (end of the molting period) or during time periods when internal body conditions were similar to those when the hair was grown (end of the breeding fast). Our results indicate that THg concentrations in blood or muscle can be translated to the other tissue type using the equations we developed, but that THg concentrations in hair were generally a poor index of internal THg concentrations except during the end of fasting periods.

California↗

USGS2009

Predicting mercury concentrations in mallard eggs from mercury in the diet or blood of adult females and from duckling down feathers

Measurements of Hg concentrations in avian eggs can be used to predict possible harm to reproduction, but it is not always possible to sample eggs. When eggs cannot be sampled, some substitute tissue, such as female blood, the diet of the breeding female, or down feathers of hatchlings, must be used. When female mallards (Anas platyrhynchos) were fed diets containing methylmercury chloride, the concentration of Hg in a sample of their blood was closely correlated with the concentration of Hg in the egg they laid the day they were bled (r 2 =0.88; p<0.001). Even when the blood sample was taken more than two weeks after an egg was laid, there was a strong correlation between Hg concentrations in female blood and eggs (r 2 =0.67; p<0.0002). When we plotted the dietary concentrations of Hg we fed to the egg-laying females against the concentrations of Hg in their eggs, the r 2 value was 0.96 (p<0.0001). When the concentrations of Hg in the down feathers of newly hatched ducklings were plotted against Hg in the whole ducklings, the r 2 value was 0.99 ( p<0.0003). Although measuring Hg in eggs may be the most direct way of predicting possible embryotoxicity, our findings demonstrate that measuring Hg in the diet of breeding birds, in the blood of egg-laying females, or in down feathers of hatchlings all can be used to estimate what concentration of Hg may have been in the egg.

Environmental Toxicology and Chemistry↗

USGS2011

Bi-phasic trends in mercury concentrations in blood of Wisconsin common loons during 1992-2010

We assessed the ecological risk of mercury (Hg) in aquatic systems by monitoring common loon ( Gavia immer ) population dynamics and blood Hg concentrations. We report temporal trends in blood Hg concentrations based on 334 samples collected from adults recaptured in subsequent years (resampled 2–9 times) and from 421 blood samples of chicks collected at lakes resampled 2–8 times 1992–2010. Temporal trends were identified with generalized additive mixed effects models and mixed effects models to account for the potential lack of independence among observations from the same loon or same lake. Trend analyses indicated that Hg concentrations in the blood of Wisconsin loons declined over the period 1992–2000, and increased during 2002–2010, but not to the level observed in the early 1990s. The best fitting linear mixed effects model included separate trends for the two time periods. The estimated trend in Hg concentration among the adult loon population during 1992–2000 was −2.6% per year, and the estimated trend during 2002–2010 was +1.8% per year; chick blood Hg concentrations decreased −6.5% per year during 1992–2000, but increased 1.8% per year during 2002–2010. This bi-phasic pattern is similar to trends observed for concentrations of methylmercury and SO 4 in lake water of an intensely studied seepage lake (Little Rock Lake, Vilas County) within our study area. A cause-effect relationship between these independent trends is hypothesized.

Wisconsin↗

USGS1981

An evaluation of blood plasma for monitoring DDE in birds of prey

Laboratory and field studies show that DDE residues in blood plasma are highly correlated with DDE in the brain, the rate of DDE exposure and the amount of DDE in eggs of free-living birds of prey. A blood sampling approach is proposed to supplement existing environmental contaminant methods available for birds of prey. Residues (ΣDDT) in plasma provide some direct information; however, a method is proposed to adjust blood plasma residues from four species of birds of prey to the estimated residues in eggs for the purpose of residue interpretation. The blood plasma-egg relationship appears to be species-independent in the four raptors studied. Two predicting equations are presented for estimating egg residues, one for plasma samples collected prior to, or within a few days of, egg laying, and another for the post-laying period. Limitations and advantages of the blood plasma monitoring approach are discussed. The approach may be particularly suitable for endangered species research.

Environmental Pollution (Series A)↗

USGS2016

Blood selenium concentrations in female Pacific black brant molting in Arctic Alaska: Relationships with age and habitat salinity

Blood samples collected from 81 female Pacific black brant ( Branta bernicla nigricans ) molting near Teshekpuk Lake, Alaska, were analyzed for selenium concentration. The concentration of selenium in blood of after second year (hatched two or more years ago) females (0.84 μg/g wet weight) was significantly greater than the concentration in second year (hatched the previous year) females (0.61 μg/g wet weight). The concentrations of selenium we found in blood of black brant were 1.5 to 2 times greater than baseline values typical of freshwater birds, but considerably lower than reported in other marine waterfowl sampled in Alaska. This finding may be attributable in part to the nearly exclusive herbivorous diet of black brant. No relationship was noted between blood selenium concentration and molting habitat salinity. We are unaware of any previous reports of blood selenium concentrations in black brant.

Alaska↗

USGS2022

Blood biochemistry and hematology of adult and chick brown pelicans in the northern Gulf of Mexico: Baseline health values and ecological relationships

The northern Gulf of Mexico supports a diverse community of nearshore seabirds during both breeding and nonbreeding periods of the annual cycle and is also a highly industrialized marine ecosystem with substantial levels of oil and gas development particularly in the west and central regions. Stakeholders in the region often assess risk to species of interest based on these differing levels of development. We collected blood samples from 81 adult and 35 chick eastern brown pelicans ( Pelecanus occidentalis carolinensis ) from 10 colonies across the northern Gulf of Mexico and used these to establish baseline values for hematology and blood biochemistry. We assessed the potential influence of body condition, sex and home range size on hematology and blood biochemistry. We also assessed potential influences of oil and gas activity by considering differing levels of oil and gas development that occur regionally throughout the study area. Although blood analyte concentrations of adults and chicks were often associated with these regional differences, the pattern we observed was not entirely consistent with the differing levels of oil and gas activity across the Gulf, suggesting that regional levels of oil and gas activity around breeding sites may not be the primary drivers of hematology and blood biochemistry. We note that baseline values or reference intervals are not available for other nearshore seabirds that breed in the northern Gulf. Given that exposure and risk may differ among this suite of species based on diet, foraging strategies and life history strategies, similar assessments and monitoring may be warranted.

Alabama, Florida, Louisiana, Mississippi, Texas↗

USGS1994

Temperature and CO2 Effects on Blood O2 Equilibria in Northern Squawfish, Ptychocheilus oregonensis

In vitro blood O 2 equilibrium curves were constructed at 9, 15, 18, and 21 °C from temperature-acclimated northern squawfish, Ptychocheilus oregonensis . At low (<1 mm Hg, 1 mm Hg = 133.32 Pa), P 50 s generally showed variable increases with temperature from 3.6 mm Hg at 9 °C to 8.7 mm Hg at 21 °C, leading to whole-blood temperature effects (Δ H , kilocalories per mole O 2 ) ranging from a low +4.4 at 15–18 °C to a peak −21.2 at 18–21 °C. High- (7.6 mm Hg) conditions decreased blood pH and increased P 50 s at each temperature (Bohr factor). Bohr factors (Φ) ranged from −0.46 at 21 °C to −0.70 at 18 °C. Considered together, Δ H and Φ values suggest an optimal temperature range of 15–18 °C for hemoglobin O 2 loading and unloading in northern squawfish. Nonbicarbonate buffer values ranged from −10.04 at 21 °C to −14.13 at 9 °C. Overall, the high O 2 affinities and hyperbolic blood O 2 equilibrium curves of northern squawfish resemble those of other large cyprinids (e.g., common carp, Cyprinus carpio , tench, Tinca tinca , Sacramento blackfish, Orthodon microlepidotus ) indicating a better ability to tolerate hypoxic environments than sympatric rainbow trout, Oncorhynchus mykiss . High northern squawfish blood O 2 capacities and Φs suggest high aerobic capacity, especially at temperatures <21 °C.

Canadian Journal of Fisheries and Aquatic Sciences↗

USGS2021

Species and population specific gene expression in blood transcriptomes of marine turtles

Background Transcriptomic data has demonstrated utility to advance the study of physiological diversity and organisms’ responses to environmental stressors. However, a lack of genomic resources and challenges associated with collecting high-quality RNA can limit its application for many wild populations. Minimally invasive blood sampling combined with de novo transcriptomic approaches has great potential to alleviate these barriers. Here, we advance these goals for marine turtles by generating high quality de novo blood transcriptome assemblies to characterize functional diversity and compare global transcriptional profiles between tissues, species, and foraging aggregations. Results We generated high quality blood transcriptome assemblies for hawksbill ( Eretmochelys imbricata ) , loggerhead ( Caretta caretta ), green ( Chelonia mydas ), and leatherback ( Dermochelys coriacea ) turtles. The functional diversity in assembled blood transcriptomes was comparable to those from more traditionally sampled tissues. A total of 31.3% of orthogroups identified were present in all four species, representing a core set of conserved genes expressed in blood and shared across marine turtle species. We observed strong species-specific expression of these genes, as well as distinct transcriptomic profiles between green turtle foraging aggregations that inhabit areas of greater or lesser anthropogenic disturbance. Conclusions Obtaining global gene expression data through non-lethal, minimally invasive sampling can greatly expand the applications of RNA-sequencing in protected long-lived species such as marine turtles. The distinct differences in gene expression signatures between species and foraging aggregations provide insight into the functional genomics underlying the diversity in this ancient vertebrate lineage. The transcriptomic resources generated here can be used in further studies examining the evolutionary ecology and anthropogenic impacts on marine turtles.

BMC Genomics↗

USGS2005

Anesthesia and blood sampling of wild big brown bats (Eptesicus fuscus) with an assessment of impacts on survival

We anesthetized and blood sampled wild big brown bats ( Eptesicus fuscus ) in Fort Collins, Colorado (USA) in 2001 and 2002 and assessed effects on survival. Inhalant anesthesia was delivered into a specially designed restraint and inhalation capsule that minimized handling and bite exposures. Bats were immobilized an average of 9.1±5.1 (SD) min (range 1–71, n =876); blood sample volumes averaged 58±12 μl (range 13–126, n =718). We randomly selected control (subject to multiple procedures before release) and treatment (control procedures plus inhalant anesthesia and 1% of body weight blood sampling) groups in 2002 to assess treatment effects on daily survival over a 14-day period for adult female and volant juvenile bats captured at maternity roosts in buildings. We monitored survival after release using passive integrated transponder tag detection hoops placed at openings to selected roosts. Annual return rates of bats sampled in 2001 were used to assess long-term outcomes. Comparison of 14-day maximum-likelihood daily survival estimates from control (86 adult females, 92 volant juveniles) and treated bats (187 adult females, 87 volant juveniles) indicated no adverse effect from anesthesia and blood sampling (juveniles: χ 2 =22.22, df=27, P >0.05; adults: χ 2 =9.72, df=18, P >0.05). One-year return rates were similar among adult female controls (81%, n =72, 95% confidence interval [CI] =70–91%), females treated once (82%, n =276, 95% CI=81–84%), and females treated twice (84%, n =50, 95% CI=74–94%). Lack of an effect was also noted in 1-yr return rates of juvenile female controls (55%, n =29, 95% CI=37–73%), juveniles treated once (66%, n =113, 95% CI=58–75%), and juveniles treated twice (71%, n =17, 95% CI=49–92%). These data suggest that anesthesia and blood sampling for health monitoring did not measurably affect survival of adult female and volant juvenile big brown bats.

Journal of Wildlife Diseases↗

USGS2021

Assessment of variation in the detection and prevalence of blood parasites among sympatrically breeding geese in western Alaska, USA

Haemosporidian parasites may impact avian health and are subject to shifts in distribution and abundance with changing ecologic conditions. Therefore, understanding variation in parasite prevalence is important for evaluating biologically meaningful changes in infection patterns and associated population level impacts. Previous research in western Alaska, US, indicated a possible increase in Leucocytozoon spp. infection between Emperor Geese ( Anser canagicus ) sampled in 1996 (<1%, n =134) and during 2011–12 (19.9%, 95% confidence interval [CI]: 3.0–36.8%, n =77); however, different detection methods were used for these estimates. Prior research in this same region identified a lack of Leucocytozoon spp. parasites (0%, n =117) in sympatrically breeding Cackling Geese ( Branta hutchinsii minima ) in 2011. We molecularly screened blood samples collected from sympatrically breeding Emperor and Cackling Geese in western Alaska during additional breeding seasons to better assess temporal and species-specific variation in the prevalence of blood parasites. We found similar prevalence estimates for Leucocytozoon spp. parasites in Emperor Goose blood samples collected in 1998 and 2014, suggesting consistent infection of Emperor Geese with blood parasites at these time points. Using samples from sympatric geese sampled during 2014, we found evidence for a higher incidence of parasites among Emperor Geese (20.3%, 95% CI: 11.8–32.7%) compared to Cackling Geese (3.6%, 95% CI: 1.1–11.0%), reinforcing the previous finding of species-specific differences in infection. Furthermore, we detected Leucocytozoon , Haemoproteus , and Plasmodium spp. blood parasites in unflighted goslings of both species, supporting the possible transmission of these parasites at western Alaska breeding grounds. Our results help to clarify that prevalence of Leucocytozoon spp. parasites have probably remained consistent among Emperor Geese breeding in western Alaska since the late 1990s and that this species may disproportionally harbor Leucocytozoon spp. compared to sympatrically breeding Cackling Geese.

Alaska↗

USGS2005

The effect of varying protein levels on blood chemistry, food consumption, and behavior of captive seaducks

The Chesapeake Bay is a primary wintering area for scoters and the long-tailed ducks (Clangia hyemalis) that migrate along the Atlantic Flyway. Recently, the Chesapeake Bay had undergone an ecosystem shift and little is known about how this is affecting the seaduck populations. We are determining what are the preferred food sources of the seaducks wintering on the Bay and analyzing the factors influencing prey selection whether it is prey composition, energy assimilated, prey availability, or a combination of any or all of these factors. We have established a captive colony of surf (Melanitta perspicillata) and white-winged scoters (Melanitta fusca) as well as long-tailed ducks at Patuxent Wildlife Research Center to allow us to examine these factors in a more controlled environment. This project contains a multitude of experiments and the resultant data will be compiled into a compartmental model on the feeding ecology of seaducks wintering on the Bay. The first experiment entailed feeding groups of each species (four ducks per pen of equal sex ratio, if possible, and four pens per species) three diets varying in percent protein levels from November to February. Each diet was randomly assigned to each pen and the amount of food consumed was recorded each day. New feed was given when all existing food was consumed. Behavioral trials and blood profiles were completed on all study birds to determine the effects of the varying diets. There were no significant differences in food consumption, blood chemistry, and behavior detected at the 5% level among the diets for all three species of interest. There was a seasonal effect determined based on the food consumption data for white-winged scoters, but not for surf scoters or long-tailed ducks. The blood profiles of the surf scoters were compared to blood profiles of wild surf scoters and a there was no difference detected at the 5% level. As a health check of the ducks an aspergillosis test was run on the blood obtained during the experiment and it was found that surf scoters are more resistant to the disease than the other species. In the next two winters natural prey items available to seaducks wintering in the Chesapeake Bay will be offered on palettes to examine preference without the additional energetic costs of diving. Assimilation efficiency trials will be run on all three species to determine amount of energy the ducks obtain from each food source. Finally, using two large aquariums, prey preference will be analyzed with the additional energetic costs of diving and searching for prey. In addition, we will determine the effect of availability of a prey item on the prey selection of seaducks. We hope the model created from these experiments will allow managers to examine the effects of changes in the benthos on the seaduck populations wintering in the Chesapeake Bay.

Book chapter↗

USGS1959

Plasmoptysis and gelatin of erythrocytes in coagulation of blood of freshwater bony fishes

1. A clot-like material which results from very rapid plasmoptysis and hydrophyllic swelling of karyoplasm of erythrocytes from freshwater fish was reported. This attribute was found to a lesser degree in erythrocytes from an amphibian, but it was almost lacking in those of an aquatic reptile. 2. Rapid clotting of fish blood with the usual clinical methods was confirmed in this work. Fish blood clotted more quickly with the slide test than with the capillary tube test. In the presence of water, complete coagulation of fish blood occurred in only 65 per cent of the time required for clotting to be completed in air. This speed is attributed to the water-erythrocyte reaction, and not to a quality of the plasma. 3. An attempt was made to correlate the findings with the need for hemostasis and blood coagulation that is peculiar to aquatic vertebrates.

Blood↗

USGS2014

Toxicity of Pb-contaminated soil to Japanese quail (Coturnix japonica) and the use of the blood-dietary Pb slope in risk assessment

This study relates tissue concentrations and toxic effects of Pb in Japanese quail ( Coturnix japonica ) to the dietary exposure of soil-borne Pb associated with mining and smelting. From 0% to 12% contaminated soil, by weight, was added to 5 experimental diets (0.12 to 382 mg Pb/kg, dry wt) and fed to the quail for 6 weeks. Benchmark doses associated with a 50% reduction in delta-aminolevulinic acid dehydratase activity were 0.62 mg Pb/kg in the blood, dry wt, and 27 mg Pb/kg in the diet. Benchmark doses associated with a 20% increase in the concentration of erythrocyte protoporphyrin were 2.7 mg Pb/kg in the blood and 152 mg Pb/kg in the diet. The quail showed no other signs of toxicity (histopathological lesions, alterations in plasma–testosterone concentration, and body and organ weights). The relation of the blood Pb concentration to the soil Pb concentration was linear, with a slope of 0.013 mg Pb/kg of blood (dry wt) divided by mg Pb/kg of diet. We suggest that this slope is potentially useful in ecological risk assessments on birds in the same way that the intake slope factor is an important parameter in risk assessments of children exposed to Pb. The slope may also be used in a tissue-residue approach as an additional line of evidence in ecological risk assessment, supplementary to an estimate of hazard based on dietary toxicity reference values.

Missouri↗

USGS2007

Concentrations of metals in blood and feathers of nestling ospreys (Pandion haliaetus) in Chesapeake and Delaware Bays

In 2000, 2001, and 2002, blood and feather samples were collected from 40–45-day-old nestling ospreys ( Pandion haliaetus ) from Chesapeake Bay and Delaware Bay and River. Concentrations of 18 metals, metalloids, and other elements were determined in these samples by inductively coupled plasma-mass spectroscopy, and Hg concentrations were measured by cold vapor atomic absorption spectroscopy. When compared to concurrent reference areas (South, West, and Rhode Rivers), mean As and Hg concentrations in blood were greater ( p < 0.05) in two of three Chesapeake Bay regions of concern (Baltimore Harbor [As: 1.18 vs. 0.548 μg/g dw], Anacostia River [Hg: 0.305 vs. 0.178 μg/g dw], and Elizabeth River [As: 0.876 vs. 0.663 μg/g dw; Hg: 0.260 vs. 0.180 μg/g dw]). Lead was detected more frequently in blood of nestlings from the highly industrialized Elizabeth River compared to the rural reference area. When compared to the concurrent reference area, mean Al, Ba, Hg, Mn, and Pb concentrations in feathers were substantially greater ( p < 0.05) in one or more Chesapeake regions of concern (Anacostia River [Al: 206 vs. 62.1 μg/g dw; Ba: 3.31 vs. 0.823 μg/g dw; Mn: 65.4 vs. 22.9 μg/g dw] and Elizabeth River [Al: 165 vs. 63.5 μg/g dw; Hg: 1.24 vs. 0.599 μg/g dw; Pb 1.47 vs. 0.543 μg/g dw]). When compared to the coastal Inland Bays reference area, feathers of nestlings from northern Delaware Bay and River had greater concentrations ( p < 0.05) of Ba (1.90 vs. 0.660 μg/g dw), Fe (258 vs. 109 μg/g dw), Mn (18.5 vs. 4.66 μg/g dw), Mo (0.130 vs. 0.040 μg/g dw), Pb (1.96 vs. 0.624 μg/g dw), and V (0.671 vs. 0.325 μg/g dw), presumably due to extensive metal-working and petroleum refinery activities. Concentrations of Hg in nestling feathers from Delaware were frequently greater than in the Chesapeake. The present findings and those of related reproductive studies suggest that concentrations of several heavy metals (e.g., Cd, Hg, Pb) in nestling blood and feathers from Chesapeake and Delaware Bays were below toxicity thresholds and do not seem to be affecting chick survival during the nestling period.

Delaware, Maryland, New Jersey, Virginia↗