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Assemblages of breeding birds as indicators of grassland condition

We developed a measure of biological integrity for grasslands (GI) based on the most influential habitat types in the Prairie Pothole Region of North Dakota. GI is based on proportions of habitat types and the relationships of these habitat types to breeding birds. Habitat types were identified by digital aerial photography, verified on the ground, and quantified using GIS, We then developed an index to GI based on the presence or abundance of breeding bird species. Species abundance data were obtained from 3 min roadside point counts at 889 points in 44, 4050 ha study plots over a 2-year period. Using a modified North American Breeding Bird Survey protocol, species were recorded in each of four quadrants at each point. Fifty species selected for analysis included all grassland species that occurred in at least 15 quadrants and all other bird species that occurred in at least 1 % of quadrants. We constructed preliminary models using data from each of the 2 years, then tested their predictive ability by cross-validation with data from the other year. These cross-validation tests indicated that the index consistently predicted grassland integrity. The final four models (presence and abundance models at 200 and 400 m scales) included only those species that were statistically significant (P ??? 0.05) in all preliminary models. Finally, we interpreted the components of the indices by examining associations between individual species and habitat types. Logistic regression identified 386 statistically significant relationships between species and habitat types at 200 and 400m scales. This method, though labor-intensive, successfully uses the presence of grassland-dependent species and absence of species associated with woody vegetation or cropland to provide an index to grassland integrity. Once regional associations of species with habitat types have been identified, such indices can be applied relatively inexpensively to monitor grassland integrity over large geographic areas. Indices like the ones presented here could be applied widely using bird abundance data that are currently being collected across the United States and southern Canada through the North American Breeding Bird Survey. ?? 2002 Elsevier Science Ltd. All rights reserved.

Ecological Indicators

Reduction of taxonomic bias in diatom species data

Inconsistency in taxonomic identification and analyst bias impede the effective use of diatom data in regional and national stream and lake surveys. In this study, we evaluated the effect of existing protocols and a revised protocol on the precision of diatom species counts. The revised protocol adjusts four elements of sample preparation, taxon identification and enumeration, and quality control (QC). We used six independent datasets to assess the effect of the adjustments on analytical outcomes. The first dataset was produced by three laboratories with a total of five analysts following established protocols (Charles et al. 2002), or their slight variations. The remaining datasets were produced by 1-3 laboratories with a total of 2-3 analysts following a revised protocol. The revised protocol included the following modifications: 1) development of coordinated pre-count voucher floras based on morphological operational taxonomic units (mOTUs), 2) random assignment of samples to analysts, 3) post-count identification and documentation of taxa (as opposed to an approach in which analysts assign names while they enumerate), and 4) increased use of QC samples. The revised protocol reduced taxonomic bias, as measured by reduction in analyst signal, and improved similarity among QC samples. Reduced taxonomic bias improves the performance of biological assessments, facilitates transparency across studies, and refines estimates of diatom species distributions.

Limnology and Oceanography: Methods

Field trials of line transect methods applied to estimation of desert tortoise abundance

We examine the degree to which field observers can meet the assumptions underlying line transect sampling to monitor populations of desert tortoises ( Gopherus agassizii ). We present the results of 2 field trials using artificial tortoise models in 3 size classes. The trials were conducted on 2 occasions on an area south of Las Vegas, Nevada, where the density of the test population was known. In the first trials, conducted largely by experienced biologists who had been involved in tortoise surveys for many years, the density of adult tortoise models was well estimated (-3.9% bias), while the bias was higher (-20%) for subadult tortoise models. The bias for combined data was -12.0%. The bias was largely attributed to the failure to detect all tortoise models on or near the transect centerline. The second trials were conducted with a group of largely inexperienced student volunteers and used somewhat different searching methods, and the results were similar to the first trials. Estimated combined density of subadult and adult tortoise models had a negative bias (-7.3%), again attributable to failure to detect some models on or near the centerline. Experience in desert tortoise biology, either comparing the first and second trials or in the second trial with 2 experienced biologists versus 16 novices, did not have an apparent effect on the quality of the data or the accuracy of the estimates. Observer training, specific to line transect sampling, and field testing are important components of a reliable survey. Line transect sampling represents a viable method for largescale monitoring of populations of desert tortoise; however, field protocol must be improved to assure the key assumptions are met.

Nevada

An approach to regional wetland digital elevation model development using a differential global positioning system and a custom-built helicopter-based surveying system

Accurate topographic data are critical to restoration science and planning for the Everglades region of South Florida, USA. They are needed to monitor and simulate water level, water depth and hydroperiod and are used in scientific research on hydrologic and biologic processes. Because large wetland environments and data acquisition challenge conventional ground-based and remotely sensed data collection methods, the United States Geological Survey (USGS) adapted a classical data collection instrument to global positioning system (GPS) and geographic information system (GIS) technologies. Data acquired with this instrument were processed using geostatistics to yield sub-water level elevation values with centimetre accuracy (±15 cm). The developed database framework, modelling philosophy and metadata protocol allow for continued, collaborative model revision and expansion, given additional elevation or other ancillary data.

Florida

Estimating raptor nesting success: old and new approaches

Studies of nesting success can be valuable in assessing the status of raptor populations, but differing monitoring protocols can present unique challenges when comparing populations of different species across time or geographic areas. We used large datasets from long-term studies of 3 raptor species to compare estimates of apparent nest success (ANS, the ratio of successful to total number of nesting attempts), Mayfield nesting success, and the logistic-exposure model of nest survival. Golden eagles (Aquila chrysaetos), prairie falcons (Falco mexicanus), and American kestrels (F. sparverius) differ in their breeding biology and the methods often used to monitor their reproduction. Mayfield and logistic-exposure models generated similar estimates of nesting success with similar levels of precision. Apparent nest success overestimated nesting success and was particularly sensitive to inclusion of nesting attempts discovered late in the nesting season. Thus, the ANS estimator is inappropriate when exact point estimates are required, especially when most raptor pairs cannot be located before or soon after laying eggs. However, ANS may be sufficient to assess long-term trends of species in which nesting attempts are highly detectable.

Journal of Wildlife Management

Assessing biological effects from highway-runoff constituents

Increased emphasis on evaluation of nonpoint-source pollution has intensified the need for techniques that can be used to discern the toxicological effects of complex chemical mixtures. In response, the use of biological assessment techniques is receiving increased regulatory emphasis. When applied with documented habitat assessment and chemical analysis, these techniques can increase our understanding of the influence of environmental contaminants on the biological integrity and ecological function of aquatic communities. The contaminants of greatest potential concern in highway runoff are those that arise from highway construction, maintenance, and use. The major contaminants of interest are deicers; nutrients; metals; petroleum-related organic compounds, such as polycyclic aromatic hydrocarbons (PAHs), benzene, toluene, ethylbenzene, and xylene (BTEX), and methyl tert -butyl ether (MTBE); sediment washed off the road surface; and agricultural chemicals used in highway maintenance. Hundreds, if not thousands, of biological endpoints (measurable responses of living organisms) may be either directly or associatively affected by contaminant exposure. Measurable effects can occur throughout ecosystem processes across the wide range of biological complexity, ranging from responses at the biochemical level to the community level. The challenge to the environmental scientist is to develop an understanding of the relationship of effects at various levels of biological organization in order to determine whether a causal relationship exists between chemical exposure and substantial ecological impairment. This report provides a brief history of the evolution of biological assessment techniques, a description of the major classes of contaminants that are of particular interest in highway runoff, an overview of representative biological assessment techniques, and a discussion of data-quality considerations. Published reports with a focus on the effects of highway runoff on the local ecosystem were reviewed to provide information on (1) the suitability of the existing data for a quantitative national synthesis, (2) the methods available to study the effects of highway runoff on local ecosystems, and (3) the potential for adverse effects on the roadside environment and receiving waters. Although many biological studies have been done, the use of different methods and a general lack of sufficient documentation precludes a quantitative national synthesis on the basis of the existing data. The Federal Highway Administration, the U.S. Environmental Protection Agency, the U.S. Geological Survey, the Intergovernmental Task Force on Monitoring Water Quality, and the National Resources Conservation Service all have developed and documented methods for assessing the effects of contaminants on ecosystems in receiving waters. These published methods can be used to formulate a set of protocols to provide consistent information from highway-runoff studies. Review of the literature indicates (qualitatively) that highway runoff (even from highways with high traffic volume) may not usually be acutely toxic. Tissue analysis and community assessments, however, indicate effects from highway- runoff sediments near discharge points (even from sites near highways with relatively low traffic volumes). At many sites, elevated concentrations of highway-runoff constituents were measured in tissues of species associated with aquatic sediments. Community assessments also indicate decreases in the diversity and productivity of aquatic ecosystems at some sites receiving highway runoff. These results are not definitive, however, and depend on many site-specific criteria that were not sufficiently documented in most of the studies reviewed.

Open-File Report

Recommended methods for range-wide monitoring of prairie dogs in the United States

One of the greatest challenges for conserving grassland, prairie scrub, and shrub-steppe ecosystems is maintaining prairie dog populations across the landscape. Of the four species of prairie dogs found in the United States, the Utah prairie dog (Cynomys parvidens) is listed under the Endangered Species Act (ESA) as threatened, the Gunnison's prairie dog (C. gunnisoni) is a candidate for listing in a portion of its range, and the black-tailed prairie dog (C. ludovicianus) and white-tailed prairie dog (C. leucurus) have each been petitioned for listing at least once in recent history. Although the U.S. Fish and Wildlife Service (USFWS) determined listing is not warranted for either the black-tailed prairie dog or white-tailed prairie dog, the petitions and associated reviews demonstrated the need for the States to monitor and manage for self-sustaining populations. In response to these findings, a multi-State conservation effort was initiated for the nonlisted species which included the following proposed actions: (1) completing an assessment of each prairie dog species in each State, (2) developing a range-wide monitoring protocol for each species using a statistically valid sampling procedure that would allow comparable analyses across States, and (3) monitoring prairie dog status every 3-5 years depending upon the species. To date, each State has completed an assessment and currently is monitoring prairie dog status; however, for some species, the inconsistency in survey methodology has made it difficult to compare data year-to-year or State-to-State. At the Prairie Dog Conservation Team meeting held in November 2008, there was discussion regarding the use of different methods to survey prairie dogs. A recommendation from this meeting was to convene a panel in a workshop-type forum and have the panel review the different methods being used and provide recommendations for range-wide monitoring protocols for each species of prairie dog. Consequently, the Western Association of Fish and Wildlife Agencies (WAFWA), in coordination with USFWS and U.S. Geological Survey (USGS), hosted a prairie dog species survey methodology workshop January 25-28, 2010 in Fort Collins, Colorado. The workshop provided all WAFWA partners and interested parties the opportunity to present their survey methodology to a review panel made up of experts in the fields of quantitative biology, population biology, species biology, and biostatistics. This report presents the panel's survey methodology recommendations for each of the four species of prairie dogs found in the United States and, for the black-tailed prairie dog, a list of action items to facilitate implementation of the recommended methodology.

Scientific Investigations Report

Inventory of Amphibians and Reptiles at Manzanar National Historic Site, California

We conducted a baseline inventory for amphibians and reptiles at Manzanar National Historic Site (MANZ), Inyo County, California, in 2002-3. Objectives for this inventory were to: 1) inventory and document the occurrence of reptile and amphibian species at MANZ, with the goal of documenting at least 90% of the species present; 2) provide one voucher specimen for each species identified; 3) provide a GIS-referenced list of sensitive species that are known to be federally- or state-listed, rare, or worthy of special consideration that occur at MANZ; 4) describe park-wide distribution of federally- or state-listed, rare, or special concern species; 5) enter all species data into the National Park Service NPSpecies database; and 6) provide all deliverables as outlined in the Mojave Network Biological Inventory Study Plan. Survey methods included time-area constrained searches, lizard line transects, general surveys, nighttime road driving, and pitfall trapping. We documented the occurrence of ten reptile species (seven lizards and three snakes), but found no amphibians. Based on our findings, as well as literature review and searches for museum specimen records, we estimate inventory completeness for Manzanar to be 50%. Although the distribution and relative abundance of common lizard species is now known well enough to begin development of a monitoring protocol for that group, additional inventory work is needed in order to establish a baseline of species occurrence of amphibians and snakes at Manzanar. Key Words: amphibians, reptiles, Manzanar National Historic Site, Inyo County, California, Owens Valley, Mojave Desert, Great Basin Desert, inventory.

Open-File Report

2023-2024 Coastal sage scrub and chaparral community monitoring for western San Diego County

Western San Diego County is dominated by shrublands supporting biologically diverse native plant and animal communities. Widespread urbanization has led to regional habitat loss and fragmentation, and many species in these shrubland communities are rare, threatened, or endangered. Large-scale, multiple-species conservation planning has resulted in a regional preserve system that focuses on these shrubland communities. Several large-scale threats are leading to type conversion from shrub-dominated to non-native invasive annual grass-dominated vegetation. To understand the changes that are occurring to native shrublands, we have developed a vegetation monitoring program with several components at multiple spatial scales, focused on quantifying coastal sage scrub (CSS) and chaparral vegetation community characteristics. Several drivers of change associated with type conversion of native shrubland to non-native annual grassland have been identified by previous research including increasing fire frequency, nitrogen deposition from air pollution, and prolonged and intense drought associated with changing climate. Loss of ecological integrity indices have been proposed as useful measures of the threat of degradation and type conversion of shrublands in San Diego County. For this study, ecological integrity is defined as a system’s ability to maintain species’ relationships and functions comparable to natural habitat in the region. Previous studies have identified the percent cover of invasive non-native annual grasses as a proxy for overall ecological degradation (loss of integrity) that is consistent across native taxonomic groups. Increased cover of non-native grass is associated with lower integrity of the shrubland vegetation community as shrub-associated plant and animal species are replaced by species preferring grassy and disturbed habitats. The objectives of this CSS and chaparral vegetation community monitoring plan are to: 1) Determine the distribution, composition, structure, and integrity of CSS and chaparral vegetation communities on conserved lands in western San Diego County, 2) Identify whether these attributes of the vegetation communities are changing over time, and 3) Evaluate relationships of known drivers of change (threats) and environmental factors in association with changes in vegetation community attributes. The CSS and chaparral vegetation community monitoring program is divided into four components: 1) vegetation mapping, 2) GIS/remote sensing office analysis of landscape-scale data, 3) permanent field plots using Unmanned Aerial Systems (UAS) and field data collections, and 4) animal and target species surveys and rapid assessment protocols. The first component, which is not detailed in this vegetation monitoring plan, aims to map vegetation communities every 10-15 years based on a classification developed for western San Diego County. High resolution aerial imagery will be used to update the 2012 vegetation map and expand it across the entire study area. The second component uses remote sensing models to annually track ecological integrity of shrublands across the study area and will include a map of areas of change and areas of stability over several decades. These landscape-scale integrity classifications will be used to analyze ecological processes, threats, and abiotic factors relative to changes to shrubland ecological integrity over time and space. The third component includes field surveys of 100 permanent plots across areas historically mapped as shrublands. Surveys in the 1930s mapped vegetation types using plot data. By using this historical classification map, we included areas that have already type-converted from shrubland to non-native annual grassland. The plots were split between CSS (55 plots) and chaparral (45 plots) and stratified into four geographical eco-subregions to guarantee coverage over small patches of habitat along the heavily developed coast. Surveys will include the collection of UAS imagery at a very high resolution. Species-level identifications will be made from the imagery based on a plant list compiled of all species detected in the plot during a thorough field survey by botanists, combined with geo-referenced samples of plant species locations. In addition, herbaceous cover will be estimated in the field using nine 1-m diameter circles (one per subplot) to obtain ocular estimates of cover for each plant species within the circle frame. Soil samples will be collected and analyzed for important element compositions. These data will be analyzed to evaluate plot-level ecological integrity based on species composition and cover. Repeated monitoring will allow evaluation of changes in vegetation attributes over time with known drivers or threats and other environmental factors. In addition, analyses will focus on indicator species and various measures of biological diversity for the vegetation communities. Finally, animal species and rare plants will be assessed using either taxa-based rapid assessment protocols or specialized species-specific protocols for rarer species. The purpose of these assessments is to document the status, habitat, and threat covariates of specific species and confirm the species composition and diversity of animal taxonomic groups (e.g., pollinators). Diversity and abundance of animal species at vegetation plots will be used to refine measures and thresholds of ecological integrity. Rapid assessment protocols for animal taxonomic groups can include multiple detection methods such as, camera traps, cover boards, and bird point counts. Pollinators will be monitored at plots using a protocol currently being developed in conjunction with but separately from this plan. Target rare plant species will be monitored using the regional Inspect and Manage (IMG) protocol that measures the status of rare plant occurrences and habitat and threat covariates over time. Species-specific animal survey methods will be refined as these species are prioritized for future monitoring. The goal of this monitoring program is to classify CSS and chaparral vegetation community integrity, identify areas of degradation across western San Diego County, and characterize drivers, and environmental factors associated with loss of ecological integrity. A combination of vegetation mapping, landscape-scale remote sensing, and field plots will be used to address all the aspects of our research questions. Data compiled and collected will be available to conservation partners to help inform future management decisions.

California

Methods of analysis by the U.S. Geological Survey National Water Quality Laboratory-Processing, taxonomy, and quality control of benthic macroinvertebrate samples

Qualitative and quantitative methods to process benthic macroinvertebrate (BMI) samples have been developed and tested by the U.S. Geological Survey?s National Water Quality Laboratory Biological Group. The qualitative processing method is based on visually sorting a sample for up to 2 hours. Sorting focuses on attaining organisms that are likely to result in taxonomic identifications to lower taxonomic levels (for example, Genus or Species). Immature and damaged organisms are also sorted when they are likely to result in unique determinations. The sorted sample remnant is scanned briefly by a second person to determine if obvious taxa were missed. The quantitative processing method is based on a fixed-count approach that targets some minimum count, such as 100 or 300 organisms. Organisms are sorted from randomly selected 5.1- by 5.1-centimeter parts of a gridded subsampling frame. The sorted remnant from each sample is resorted by a second individual for at least 10 percent of the original sort time. A large-rare organism search is performed on the unsorted remnant to sort BMI taxa that were not likely represented in the sorted grids. After either qualitatively or quantitatively sorting the sample, BMIs are identified by using one of three different types of taxonomic assessment. The Standard Taxonomic Assessment is comparable to the U.S. Environmental Protection Agency Rapid Bioassessment Protocol III and typically provides Genus- or Species-level taxonomic resolution. The Rapid Taxonomic Assessment is comparable to the U.S. Environmental Protection Agency Rapid Bioassessment Protocol II and provides Familylevel and higher taxonomic resolution. The Custom Taxonomic Assessment provides Species-level resolution whenever possible for groups identified to higher taxonomic levels by using the Standard Taxonomic Assessment. The consistent use of standardized designations and notes facilitates the interpretation of BMI data within and among water-quality studies. Taxonomic identifications are quality assured by verifying all referenced taxa and randomly reviewing 10 percent of the taxonomic identifications performed weekly by Biological Group taxonomists. Taxonomic errors discovered during this review are corrected. BMI data are reviewed for accuracy and completeness prior to release. BMI data are released phylogenetically in spreadsheet format and unprocessed abundances are corrected for laboratory and field subsampling when necessary.

Open-File Report

Report of the Interagency biological methods workshop

The U.S. Geological Survey hosted the Interagency Biological Methods Workshop in Reston, Virginia, during June 22-23, 1993. The purposes of the workshop were to (1) promote better communication among Federal agencies that are using or developing biological methods in water-quality assessment programs for streams and rivers, and (2) facilitate the sharing of data and interagency collaboration. The workshop was attended by 45 biologists representing numerous Federal agencies and programs, and a few regional and State programs that were selected to provide additional perspectives. The focus of the workshop was community assessment methods for fish, invertebrates, and algae; physical habitat characterization; and chemical analyses of biological tissues. Charts comparing program objectives, design features, and sampling methods were compiled from materials that were provided by participating agencies prior to the workshop and formed the basis for small workgroup discussions. Participants noted that differences in methods among programs were often necessitated by differences in program objectives. However, participants agreed that where programs have identified similar data needs, the use of common methods is beneficial. Opportunities discussed for improving data compatibility and information sharing included (1) modifying existing methods, (2) adding parameters, (3) improving access to data through shared databases (potentially with common database structures), and (4) future collaborative efforts that range from research on selected protocol questions to followup meetings and continued discussions.

Open-File Report

The potential for citizen science to produce reliable and useful information in ecology

We examined features of citizen science that influence data quality, inferential power, and usefulness in ecology. As background context for our examination, we considered topics such as ecological sampling (probability based, purposive, opportunistic), linkage between sampling technique and statistical inference(designbased,modelbased),andscientificparadigms(confirmatory,exploratory).Wedistinguished several types of citizen science investigations, from intensive research with rigorous protocols targeting clearly articulated questions to mass-participation internet-based projects with opportunistic data collection lacking samplingdesign,andexaminedoverarchingobjectives,design,analysis,volunteertraining,andperformance. We identified key features that influence data quality: project objectives, design and analysis, and volunteer training and performance. Projects with good designs, trained volunteers, and professional oversight can meet statistical criteria to produce high-quality data with strong inferential power and therefore are well suited for ecological research objectives. Projects with opportunistic data collection, little or no sampling design, and minimal volunteer training are better suited for general objectives related to public education or data exploration because reliable statistical estimation can be difficult or impossible. In some cases, statistically robust analytical methods, external data, or both may increase the inferential power of certain opportunistically collected data. Ecological management, especially by government agencies, frequently requires data suitable for reliable inference. With standardized protocols, state-of-the-art analytical methods, and well-supervised programs, citizen science can make valuable contributions to conservation by increasing the scope of species monitoring efforts. Data quality can be improved by adhering to basic principles of data collection and analysis, designing studies to provide the data quality required, and including suitable statistical expertise, thereby strengthening the science aspect of citizen science and enhancing acceptance by the scientific community and decision makers.

Conservation Biology

Review and development of best practices for toxicity tests with dreissenid mussels

Since their introduction to North America in the 1980s, research to develop effective control tools for invasive mussels ( Dreissena polymorpha and D. rostriformis bugensis ) has been ongoing across various research institutions using a range of testing methods. Inconsistencies in experimental methods and reporting present challenges for comparing data, repeating experiments, and applying results. The Invasive Mussel Collaborative established the Toxicity Testing Work Group (TTWG) in 2019 to identify “best practices” and guide development of a standard framework for dreissenid mussel toxicity testing protocols. We reviewed the literature related to laboratory-based dreissenid mussel toxicity tests and determined the degree to which standard guidelines have been used and their applicability to dreissenid mussel testing. We extracted detailed methodology from 99 studies from the peer-reviewed and gray literature and conducted a separate analysis for studies using presettlement and postsettlement mussels. We identified specific components of methods and approaches that could be refined or standardized for dreissenid mussels. These components included species identification, collection methods, size/age class distinction, maintenance practices, testing criteria, sample size, response measures, reporting parameters, exposure methods, and mortality criteria. We consulted experts in the field of aquatic toxicology and dreissenid mussel biology on our proposed. The final recommendations contained in the present review are based on published standard guidelines, methods reported in the published and gray literature, and the expertise of TTWG members and an external panel. In addition, our review identifies research needs for dreissenid mussel testing including improved methods for early–life stage testing, comparative data on life stages and between dreissenid mussel species, inclusion of a reference toxicant, and additional testing of nontarget species (i.e., other aquatic organisms). Environ Toxicol Chem 2023;42:1649–1666. © 2023 His Majesty the King in Right of Canada. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. Reproduced with the permission of the Minister of Environment and Climate Change Canada. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.

Environmental Toxicology and Chemistry

A Synopsis of Technical Issues of Concern for Monitoring Trace Elements in Highway and Urban Runoff

Trace elements, which are regulated for aquatic life protection, are a primary concern in highway- and urban-runoff studies because stormwater runoff may transport these constituents from the land surface to receiving waters. Many of these trace elements are essential for biological activity and become detrimental only when geologic or anthropogenic sources exceed concentrations beyond ranges typical of the natural environment. The Federal Highway Administration and State Transportation Agencies are concerned about the potential effects of highway runoff on the watershed scale and for the management and protection of watersheds. Transportation agencies need information that is documented as valid, current, and scientifically defensible to support planning and management decisions. There are many technical issues of concern for monitoring trace elements; therefore, trace-element data commonly are considered suspect, and the responsibility to provide data-quality information to support the validity of reported results rests with the data-collection agency. Paved surfaces are fundamentally different physically, hydraulically, and chemically from the natural surfaces typical of most freshwater systems that have been the focus of many traceelement- monitoring studies. Existing scientific conceptions of the behavior of trace elements in the environment are based largely upon research on natural systems, rather than on systems typical of pavement runoff. Additionally, the logistics of stormwater sampling are difficult because of the great uncertainty in the occurrence and magnitude of storm events. Therefore, trace-element monitoring programs may be enhanced if monitoring and sampling programs are automated. Automation would standardize the process and provide a continuous record of the variations in flow and water-quality characteristics. Great care is required to collect and process samples in a manner that will minimize potential contamination or attenuation of trace elements and other sources of bias and variability in the sampling process. Trace elements have both natural and anthropogenic sources that may affect the sampling process, including the sample-collection and handling materials used in many trace-element monitoring studies. Trace elements also react with these materials within the timescales typical for collection, processing and analysis of runoff samples. To study the characteristics and potential effects of trace elements in highway and urban runoff, investigators typically sample one or more operationally defined matrixes including: whole water, dissolved (filtered water), suspended sediment, bottom sediment, biological tissue, and contaminant sources. The sampling and analysis of each of these sample matrixes can provide specific information about the occurrence and distribution of trace elements in runoff and receiving waters. There are, however, technical concerns specific to each matrix that must be understood and addressed through use of proper collection and processing protocols. Valid protocols are designed to minimize inherent problems and to maximize the accuracy, precision, comparability, and representativeness of data collected. Documentation, including information about monitoring protocols, quality assurance and quality control efforts, and ancillary data also is necessary to establish data quality. This documentation is especially important for evaluation of historical traceelement monitoring data, because trace-element monitoring protocols and analysis methods have been constantly changing over the past 30 years.

Open-File Report

Survey protocols drive heterogeneity in occupancy estimates that cannot be standardized using post hoc analyses

Site occupancy models are widely used to estimate species distributions from presence–nondetection data, yet for mobile animals, the true proportion of sites occupied varies in time. Thus, true occupancy and estimates generated from different protocols are affected by temporal characteristics of survey design. Here we tested whether we could standardize occupancy estimates across different sampling protocols with a post hoc statistical test. Using an individual-based movement model of Wood Thrush ( Hylocichla mustelina ), we simulated point count surveys across 3000 populations under 90 spatially and temporally variable sampling protocols. For each simulation, we fit a standard occupancy model, then used minute-level detections to truncate surveys and generate occupancy estimates across increasing survey lengths. We fit asymptotic models to these series to estimate intercepts (instantaneous occupancy) and asymptotes (longer-term occupancy) and evaluated how well they approximated true instantaneous, daily and seasonal occupancy. We were unable to recover instantaneous or seasonal occupancy from any approach. Both standard occupancy models and asymptotic regression (the top-performing asymptotic model) most closely approximated daily occupancy; although asymptotic regression produced slightly less biased estimates, it showed substantially greater variability, resulting in greater overall error. Our results show that post hoc analytical approaches cannot, at present, reliably standardize occupancy estimates across heterogeneous survey designs. Instead, ensuring biologically informative and comparable estimates will depend on standardized survey protocols. Practical implication : Monitoring programs using presence–nondetection data should prioritize consistent survey protocols when estimating occupancy for mobile species, with a focus on daily occupancy as the most attainable and useful metric. Although the highest accuracy and precision were achieved under the most intensive survey protocols, for point counts of Wood Thrush and similar species, four consecutive 15–20 min surveys provide near-optimal precision and can be completed in a single visit, making them well suited for estimating relative occupancy under logistical constraints. Three 20-min surveys spaced ~24 h apart closely approximate daily occupancy while maintaining reasonable precision. Developing similar protocols for other species and survey methods will be critical to ensuring comparable occupancy estimates across space and time.

Ecological Solutions and Evidence

A proposed international watershed research network

An “International Watershed Research Network” is to be an initial project of the Sino-U. S. Centers for Soil and Water Conservation and Environmental Protection. The Network will provide a fundamental database for research personnel of the Centers, as well as of the global research community, and is viewed as an important resource for their successful operation. Efforts are under way to (a) identify and select candidate watersheds, (b) develop standards and protocols for data collection and dissemination, and (c) specify other data sources on erosion, sediment transport, hydrology, and ancillary information of probable interest and use to participants of the Centers. The initial focus of the Network will be on water-deficient areas. Candidate watersheds for the Network are yet to be determined although likely selections include the Ansai Research Station, northern China, and the Walnut Gulch Experimental Watershed, Arizona, USA. The Network is to be patterned after the Vigil Network, an open-ended group of global sites and small drainage basins for which Internet-accessible geomorphic, hydrologic, and biological data are periodically collected or updated. Some types of data, using similar instruments and observation methods, will be collected at all watersheds selected for the Network. Other data from the watersheds that may reflect individual watershed characteristics and research objectives will be collected as well.

Conference Paper

Scaling-up camera traps: monitoring the planet's biodiversity with networks of remote sensors

Countries committed to implementing the Convention on Biological Diversity's 2011–2020 strategic plan need effective tools to monitor global trends in biodiversity. Remote cameras are a rapidly growing technology that has great potential to transform global monitoring for terrestrial biodiversity and can be an important contributor to the call for measuring Essential Biodiversity Variables. Recent advances in camera technology and methods enable researchers to estimate changes in abundance and distribution for entire communities of animals and to identify global drivers of biodiversity trends. We suggest that interconnected networks of remote cameras will soon monitor biodiversity at a global scale, help answer pressing ecological questions, and guide conservation policy. This global network will require greater collaboration among remote-camera studies and citizen scientists, including standardized metadata, shared protocols, and security measures to protect records about sensitive species. With modest investment in infrastructure, and continued innovation, synthesis, and collaboration, we envision a global network of remote cameras that not only provides real-time biodiversity data but also serves to connect people with nature.

Frontiers in Ecology and the Environment

Natural resource mitigation, adaptation and research needs related to climate change in the Great Basin and Mojave Desert

This report synthesizes the knowledge, opinions, and concerns of many Federal and State land managers, scientists, stakeholders, and partners from a workshop, held at the University of Nevada, Las Vegas, on April 20-22, 2010. Land managers, research scientists, and resource specialists identified common concerns regarding the potential effects of climate change on public lands and natural resources in the Great Basin and Mojave Desert and developed recommendations for mitigation, adaptation, and research needs. Water and, conversely, the effects of drought emerged as a common theme in all breakout sessions on terrestrial and aquatic species at risk, managing across boundaries, monitoring, and ecosystem services. Climate change models for the southwestern deserts predict general warming and drying with increasing precipitation variability year to year. Scientists noted that under these changing conditions the past may no longer be a guide to the future in which managers envision increasing conflicts between human water uses and sustaining ecosystems. Increasing environmental stress also is expected as a consequence of shifting ecosystem boundaries and species distributions, expansion of non-native species, and decoupling of biotic mutualisms, leading to increasingly unstable biologic communities. Managers uniformly expressed a desire to work across management and agency boundaries at a landscape scale but conceded that conflicting agency missions and budgetary constraints often impede collaboration. More and better science is needed to cope with the effects of climate change but, perhaps even more important is the application of science to management issues using the methods of adaptive management based on long-term monitoring to assess the merits of management actions. Access to data is essential for science-based land management. Basic inventories, spatial databases, baseline condition assessments, data quality assurance, and data sharing were identified as top information priorities by all participants at this workshop. Optimizing the utility of ecosystem monitoring data will require standardizing monitoring protocols across agencies. Better communication among researchers and managers and cooperation through partnerships to manage resources across boundaries were emphasized as necessary for adapting to changing climatic conditions. However, even these strategies may be insufficient unless policy mandates, agency missions, and funding are coordinated at a high level.

Scientific Investigations Report