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Application of a fluidized bed reactor charged with aragonite for control of alkalinity, pH and carbon dioxide in marine recirculating aquaculture systems

Control of alkalinity, dissolved carbon dioxide (dCO 2 ), and pH are critical in marine recirculating aquaculture systems (RAS) in order to maintain health and maximize growth. A small-scale prototype aragonite sand filled fluidized bed reactor was tested under varying conditions of alkalinity and dCO 2 to develop and model the response of dCO 2 across the reactor. A large-scale reactor was then incorporated into an operating marine recirculating aquaculture system to observe the reactor as the system moved toward equilibrium. The relationship between alkalinity dCO 2 , and pH across the reactor are described by multiple regression equations. The change in dCO 2 across the small-scale reactor indicated a strong likelihood that an equilibrium alkalinity would be maintained by using a fluidized bed aragonite reactor. The large-scale reactor verified this observation and established equilibrium at an alkalinity of approximately 135 mg/L as CaCO 3 , dCO 2 of 9 mg/L, and a pH of 7.0 within 4 days that was stable during a 14 day test period. The fluidized bed aragonite reactor has the potential to simplify alkalinity and pH control, and aid in dCO 2 control in RAS design and operation. Aragonite sand, purchased in bulk, is less expensive than sodium bicarbonate and could reduce overall operating production costs.

Aquacultural Engineering

Phosphorus removal from aquaculture effluents at the Northeast Fishery Center in Lamar, Pennsylvania using iron oxide sorption media

Three different iron oxide-based sorption media samples were tested for removal of phosphorus (P) from fish hatchery effluents using fixed bed processing. Two of the media samples were derived from residuals produced by the treatment of acid mine drainage, which were then compared to granular ferric hydroxide (GFH), a commercially available sorption medium. All of the media types removed from 50 to 70% of the P from the incoming aquaculture wastewater over 70–175 days of operation without regeneration. In some of the sorption trials, the GFH media showed superior adsorption in the earlier stages of the trial, but the GFH appeared to reach saturation more quickly, so that media performance was similar – at about 60% removal of P – over a longer time period of 175 days. Media regeneration tests were also conducted for both the commercial and mine drainage media, and demonstrated longer term performance, with overall P removal of 50–55%, over 223 days of total operation, with the advantages of phosphorus recycle and media reuse.

Pennsylvania

Comparison of hydraulics and particle removal efficiencies in a mixed cell raceway and Burrows pond rearing system

We compared the hydrodynamics of replicate experimental mixed cell and replicate standard Burrows pond rearing systems at the Dworshak National Fish Hatchery, ID, in an effort to identify methods for improved solids removal. We measured and compared the hydraulic residence time, particle removal efficiency, and measures of velocity using several tools. Computational fluid dynamics was used first to characterize hydraulics in the proposed retrofit that included removal of the traditional Burrows pond dividing wall and establishment of four counter rotating cells with appropriate drains and inlet water jets. Hydraulic residence time was subsequently established in the four full scale test tanks using measures of conductivity of a salt tracer introduced into the systems both with and without fish present. Vertical and horizontal velocities were also measured with acoustic Doppler velocimetry in transects across each of the rearing systems. Finally, we introduced ABS sinking beads that simulated fish solids then followed the kinetics of their removal via the drains to establish relative purge rates. The mixed cell raceway provided higher mean velocities and a more uniform velocity distribution than did the Burrows pond. Vectors revealed well-defined, counter-rotating cells in the mixed cell raceway, and were likely contributing factors in achieving a relatively high particle removal efficiency-88.6% versus 8.0% during the test period. We speculate retrofits of rearing ponds to mixed cell systems will improve both the rearing environments for the fish and solids removal, improving the efficiency and bio-security of fish culture. We recommend further testing in hatchery production trials to evaluate fish physiology and growth.

Idaho

Investigating the influence of nitrate nitrogen on post-smolt Atlantic salmon Salmo salar reproductive physiology in water recirculation aquaculture systems

A major issue affecting land-based, closed containment Atlantic salmon Salmo salar growout production in water recirculation aquaculture systems (RAS) is precocious male maturation, which can negatively impact factors such as feed conversion, fillet yield, and product quality. Along with other water quality parameters, elevated nitrate nitrogen (NO 3 -N) has been shown to influence the reproductive development and endogenous sex steroid production in a number of aquatic animal species, including Atlantic salmon. We sought to determine whether elevated NO 3 -N in RAS can influence early maturation in post-smolt Atlantic salmon in an 8-month trial in replicated freshwater RAS. Post-smolt Atlantic salmon (102 ± 1 g) were stocked into six RAS, with three RAS randomly selected for dosing with high NO 3 -N (99 ± 1 mg/L) and three RAS set for low NO 3 -N (10 ± 0 mg/L). At 2-, 4-, 6-, and 8-months post-stocking, 5 fish were randomly sampled from each RAS, gonadosomatic index(GSI) data were collected, and plasma was sampled for 11-ketotestosterone (11-KT) quantification. At 4- and 8-months post-stocking, samples of culture tank and spring water (used as “makeup” or replacement water) were collected and tested for a suite of 42 hormonally active compounds using liquid chromatography/mass spectrometry , as well as for estrogenicity using the bioluminescent yeast estrogen screen (BLYES) reporter system. Finally, at 8-months post-stocking 8–9 salmon were sampled from each RAS for blood gas and chemistry analyses, and multiple organ tissues were sampled for histopathology evaluation. Overall, sexually mature males were highly prevalent in both NO 3 -N treatment groups by study’s end, and there did not appear to be an effect of NO 3 -N on male maturation prevalence based on grilse identification, GSI, and 11-KT results, indicating that other culture parameters likely instigated early maturation. No important differences were noted between treatment groups for whole blood gas and chemistry parameters, and no significant tissue changes were noted on histopathology. No hormones, hormone conjugates, or mycotoxins were detected in any water samples; phytoestrogens were generally detected at low levels but were unrelated to NO 3 -N treatment. Finally, low-level estrogenicity was detected in RAS water, but a NO 3 -N treatment effect could not be determined. The major findings of this study are i) the NO 3 -N treatments did not appear to be related to the observed male maturation, and ii) the majority of hormonally active compounds were not detectable in RAS water.

Aquacultural Engineering

Performance and application of a fluidized bed limestone reactor designed for control of alkalinity, hardness and pH at the Warm Springs Regional Fisheries Center

Springs serving the Warm Springs Regional Fisheries Center, Warm Springs, Georgia, have pH, alkalinity, and hardness levels thatlie under the range required for successful fish propagation while free CO 2 is well above allowable targets. We evaluate a pretreatment process that exploits limestone’s (CaCO 3 ) ability to react away hydrogen ions (H + ) and carbon dioxide (CO 2 ) while increasing alkalinity (HCO 3 − ) and calcium (Ca2+) concentrations, i.e. CaCO 3 + H + ↔ HCO 3 − + Ca 2+ CaCO 3 + CO 2 + H 2 O ↔ Ca 2+ + 2HCO 3 − Limestone sand was tested in both pilot and full scale fluidized bed reactors (CycloBio®). We first established the bed expansion characteristics of three commercial limestone products then evaluated the effect of hydraulic flux and bed height on dissolution rate of a single selected product (Type A16 × 120). Pilot scale testing at 18C showed limestone dissolution rates were relatively insensitive to flux over the range 1.51–3.03 m 3 /min/m 2 but were sensitive (P < 0.001; R 2 = 0.881) to changes in bed height (BH, cm) over the range 83–165 cm following the relation: (Alkalinity, mg/L) = 123.51 − (3788.76 (BH)). Differences between filtered and non-filtered alkalinity were small(P > 0.05) demonstrating that limestone was present in the reactor effluent primarily in the form of dissolved Ca(HCO 3 ) 2 . Effluent alkalinity exceeded our target level of 50 mg/L under most operating conditions evaluated with typical pilot scale values falling within the range of 90–100 mg/L despite influent concentrations of about 4 mg/L. Concurrently, CO 2 fell from an average of 50.6 mg/L to 8.3 mg/L (90%), providing for an increase in pH from 5.27 to a mean of 7.71. The ability of the test reactor to provide changes in water chemistry variables that exceeded required changes allowed for a dilution ratio of 0.6. Here, alkalinity still exceeded 50 mg/L, the CO 2 concentration remained well below our limit of 20 mg/L (15.4 mg/L) and the pH was near neutral (7.17). Applying the dilution ratio of 0.6 in a full scale treatment plant at the site reduced by 40% the volume of spring water that is directed through each of three parallel reactors that combined react away 49,000 kg of limestone/yr.

Aquacultural Engineering

Hydraulic characteristics of a rectangular mixed-cell rearing unit

We describe a fish rearing unit modification that establishes mixed flow reactor (MFR) behavior in a rectangular vessel so as to eliminate metabolite concentration gradients, increase current velocities and improve solids scour at low water exchange rates. A standard raceway section 14.5 m long was modified to create six counter-rotating mixed cells, each 2.4 m wide by 2.4 m long. Cells receive water from vertical pipe sections extending to the tank floor and positioned in the corners of the cells. Vertical pipe sections were fabricated with jet ports that direct water into the cells tangentially to establish rotary circulation. Water exits each cell through a centrally located floor drain. Hydraulic characteristics of the tank are described based on an analysis of residence time distribution, current velocities and cell-to-cell water exchange rates. The latter was calculated based on a proposed hydraulics model and tracer concentrations established at a steady state. Vessel dispersion numbers measured with water exchange rates of 1.0 and 1.3 per hour, indicate tank performance approximates that of a MFR with the active tank volume representing 77.9–100% of total tank volume. Water velocities measured at 216 stations averaged 0.14, 0.12 and 0.12 m/s for the tank surface, mid-depth and near bottom regions. The benthic shear stress calculated given surface velocity was 0.04 N/m 2 . This was sufficient to scour and purge fecal solids produced by 2010 kg of lake trout that averaged 44.3 cm in total length. Cell interaction was significant with cell-to-cell exchange rates averaging 3.9 times the tank inflow rate. Energy requirements of the rearing unit were kept low (1.32 m water gauge pressure; 6.7 W/m 3 ) through use of a large number of low velocity jets.

Aquacultural Engineering

Evaluation of chemical coagulation-flocculation aids for the removal of suspended solids and phosphorus from intensive recirculating aquaculture effluent discharge

An evaluation of two commonly used coagulation-flocculation aids (alum and ferric chloride) was conducted for the supernatant overflow from settling cones used to treat the effluent from microscreen filters in an intensive recirculating aquaculture system. In addition to determining the effectiveness of these aids in removing both suspended solids and phosphorus, a systematic testing of the variables normally encountered in the coagulation-flocculation process was performed. Tests were carried out to evaluate the dosages and conditions (mixing and flocculation stirring speeds, durations, and settling times) required to achieve optimum waste capture. The orthophosphate removal efficiency for alum and ferric chloride were 89 and 93%, respectively, at a dosage of 90 mg/l. Optimum turbidity removal was achieved with a 60 mg/l dosage for both alum and ferric chloride. Both alum and ferric. chloride demonstrated excellent removal of suspended solids from initial TSS values of approximately 100-10 mg/l at a dosage of 90 mg/l. Flocculation and mixing speed played only a minor role in the removal efficiencies for both orthophosphates and suspended solids. Both coagulation-flocculation aids also exhibited excellent settling characteristics, with the majority of the floc quickly settling out in the first 5 min. ?? 2003 Elsevier B.V. All rights reserved.

Aquacultural Engineering

Modeling gas transfer in a spray tower oxygen absorber

A computer model characterizing the performance of a spray tower oxygen absorption system was developed based on finite difference mass transfer calculations. Performance was assessed in terms of oxygen utilization, transfer efficiency, and economy. Pilot scale tests verified model assumptions and performance predictions. Simulation runs indicated spray tower head and oxygen feed requirements for desired changes in dissolved oxygen (DO) exceeded those required for packed column equipment. Spray tower performance was improved by increasing hydraulic loading from 35 to 85 kg m −2 s −1 and by increasing tower height from 1·25 to 2·50 m. The effluent DO concentration that minimized variable costs of oxygen transfer was lower in the spray tower than in the packed tower, indicating clean water use of the spray tower will be limited to moderate effluent DO requirement applications (DO <20 mg l −1 ).

Aquacultural Engineering

Importance of rearing-unit design and stocking density to the behavior, growth and metabolism of lake trout (Salvelinus namaycush)

Juvenile lake trout ( Salvelinus namaycush ) were held at different stocking densities (48 and 96 kg m −3 ) in rearing-units of different design (rectangular plug-flow, circular and cylindrical cross-flow) and the effects on behavior, growth and metabolism were examined. Ambient light levels and current velocities were measured in each of three tank sectors (upstream, middle and downstream) to determine their relative contributions to fish behavior and distribution. Rearing-unit design affected orientation to current, contact with surfaces, agonistic responses, turn rates and avoidance of or contact with other fish. Stocking density primarily affected contact with tank surfaces, avoidance and contact with other fish. Fish in circular tanks distributed themselves uniformly, whereas those in plug-flow tanks crowded upstream and those in cross-flow tanks congregated downstream. Stocking density affected fish distribution most in cross-flow and least in circular tanks. Ambient light, current velocity and total in situ aggression modified fish distribution at low stocking density, but at high density the effects of current velocity and total aggression were undetectable. Growth and food conversion were best in plug-flow, followed by cross-flow and then circular tanks. Net ammonia excretion and oxygen consumption were highest in circular tanks and lowest in cross-flow (NH 3 ) or cross-flow and plug-flow (O 2 ) tanks. Ammonia production was correlated with stocking density, but oxygen consumption was unrelated to density. Results demonstrate the interrelatedness of abiotic and biotic factors in fish culture and the ability to control fish behavior, growth and metabolism through the alteration of several physical and biological variables in the rearing unit.

Aquacultural Engineering

Evaluating deterrent locations and sequence in the Tennessee and Cumberland Rivers and the Tennessee–Tombigbee Waterway to minimize invasive carp occupancy and abundance

Invasive carps, specifically silver carp ( Hypophthalmichthys molitrix ), bighead carp ( H . nobilis ), grass carp ( Ctenopharyngodon idella ), and black carp ( Mylopharyngodon piceus ), have proliferated in the Mississippi River Basin owing to escapes from aquaculture facilities and intentional releases. In the Water Resources and Development Act (WRDA) of 2020 Sec. 509, Congress directed the U.S. Army Corps of Engineers to work with the Tennessee Valley Authority and other relevant agencies with deterrent projects to implement as many as 10 deterrent projects intended to manage and prevent the spread of invasive carp in the Tennessee and Cumberland River subbasins. The WRDA was amended in 2022 to include that at least one location must be situated on the Tennessee–Tombigbee Waterway. This report documents a structured decision-making process that engaged State and Federal agencies to evaluate alternative deterrent site sequences at specified lock and dam complexes on the Tennessee River, Cumberland River, and the Tennessee–Tombigbee Waterway. State and Federal agencies participated in a series of virtual and face-to-face meetings to structure the problem, expand the models used in previous decision analyses for the Tennessee River, and define management objectives. Potential deterrent sites were restricted to the downstream locations on the Tennessee River ( n =3), Cumberland River ( n =2), and the Tennessee–Tombigbee Waterway ( n =10). Only considering 15 sites allowed all feasible deterrent site combinations and sequences to be evaluated. Invasive carp relative abundance was projected for the Tennessee River, Cumberland River, and Tennessee–Tombigbee Waterway management units for 20 years using a simulation model. The deterrent site sequences were ranked based on the system-level invasive carp relative abundance and distribution in year 20. The unique downstream expansion of invasive carp through the Tennessee–Tombigbee Waterway was important to the interest group, but downstream movement rates were unknown; therefore, several downstream movement rates were evaluated, and the outcomes were used to rank deterrent site sequences. Additionally, the analysis incorporated two scenarios involving the retention and removal of an experimental deterrent at Barkley Lock on the Cumberland River. The results of the deterrent site sequences varied among downstream movement rates, with Tennessee–Tombigbee Waterway deterrent locations installed earlier in highly ranked sequences with increasing downstream movement rates. This analysis was time-limited owing to agency needs and represents Phase 1 of this project. Phase 2 expands Phase 1 to address additional uncertainties and more holistic management objectives and strategies.

Alabama, Georgia, Kentucky, Mississippi, Tennessee

Invasive crayfish in the Pacific Northwest

Invasive species directly threaten freshwater biodiversity, particularly in regions of high aquatic richness like the Pacific Northwest (PNW). Crayfish are among the most impactful of aquatic invasive species. Invasive crayfish are considered ecosystem engineers due to their ability to alter basic wetland properties, such as reducing vegetation and bank integrity and increasing turbidity. In areas where invasion is advanced, crayfish pose major economic and ecological problems. Crayfish have been widely introduced for aquaculture and can become established in a wide range of habitat conditions. They also may be spread by anglers who use them as bait. Several non-native crayfish are established in the PNW, but the extent of their invasion is not well known. At least two groups are known from scattered sites in the PNW, and both have proven problematic for native species in other parts of the world: Red swamp crayfish ( Procambarus clarkii ) and several members of the genus Orconectes . Both groups are native to areas of the eastern United States. Both are identified globally as invasives of high concern and appear on the Oregon Department of Fish and Wildlife's "10 Most Unwanted" and the U.S. Forest Service's "Primary Species of Concern" lists for stream systems in the PNW. Despite the presence of introduced crayfish in the PNW and their high potential for negative effects, the scope of their invasion and effects on aquatic systems are not well known. The U.S. Geological Survey (USGS), along with local groups and state agencies, is working to clarify crayfish distribution and to outline which basins may not yet be invaded. Other goals are to improve understanding of habitat associations of invasive crayfish and their potential effects on native crayfish.

Pacific Northwest

Methods for evaluating potential sources of chloride in surface waters and groundwaters of the conterminous United States

Chloride exists as a major ion in most natural waters, but many anthropogenic sources are increasing concentrations of chloride in many receiving waters. Although natural concentrations in continental waters can be as high as 200,000 milligrams per liter, chloride concentrations that are suitable for freshwater ecology, human consumption, and agricultural and industrial water uses commonly are on the order of 10 to 1,000 milligrams per liter. &ldquo;Road salt&rdquo; frequently is identified as the sole source of anthropogenic chloride, but only about 30 percent of the salt consumed and released to the environment is used for deicing. Furthermore, several studies in Southern States where the use of deicing salt is minimal also show anthropogenic chloride in rising concentrations and in strong correlation to imperviousness and road density. This is because imperviousness and road density also are strongly correlated to population density. The term &ldquo;road salt&rdquo; is a misnomer because deicers applied to parking lots, sidewalks, and driveways can be a substantial source of chloride in some catchments because these land covers are comparable to roadways as a percentage of the total impervious area and commonly receive higher salt application rates than some roadways. Other sources of anthropogenic chloride include wastewater, dust control on unpaved roads, fertilizer, animal waste, irrigation, aquaculture, energy production wastes, and landfill leachates. The assumption that rising chloride concentrations in surface water or groundwater is indicative of contamination by deicing chemicals rather than one or more other potential sources may preclude the identification of toxic, carcinogenic, mutagenic, or endocrine-disrupting contaminants that are associated with many sources of elevated chloride concentrations. Once the sources of anthropogenic chloride in an area of interest have been identified and measured, water and solute budgets can be estimated to guide decisionmakers to identify and apply potential mitigation measures that can reduce the problem. Scientists, engineers, regulators, and decisionmakers need information about potential sources of chloride, water and solute budgets, and methods for collecting water-quality data to help identify potential sources. This information is needed to evaluate potential sources of chloride in areas where chloride may have adverse ecological effects or may degrade water supplies used for drinking water, agriculture, or industry. Knowledge of potential sources will help decisionmakers identify the best mitigation measures to reduce the total background chloride load, thereby reducing the potential for water-quality exceedances that occur because of superposition on rising background concentrations. Also, knowledge of potential sources may help decisionmakers identify the potential for the presence of contaminants that have toxic, carcinogenic, mutagenic, or endocrine-disrupting effects at concentrations that are lower by orders of magnitude than the chloride concentrations in the source water. This report is a comprehensive synthesis of relevant information, but it is not the result of an exhaustive search for literature on each topic. The potential adverse effects of chloride on infrastructure and the environment are not discussed in this report because these issues have been extensively documented elsewhere.

Open-File Report

Technological innovations in aquaculture

Technology in aquaculture has expanded at a phenomenal rate during the last 50 to 100 years. Based on both this past progress and the present technological advances being made in our society, it is reasonable to expect aquaculturists to profit from the rapid technological advances being made in both hardware and biological systems. These advances will result as spinoffs from medicine, from analytical chemistry, and most importantly, from the evolution of new consumer products such as auto-focus cameras, electronic watches, and microprocessor-monitored automobiles. We can expect to use economical, reliable instrumentation capable of environmental monitoring for predictive purposes and instrumentation that will contain alarm and control circuits. Genetic engineering will result in biological organisms that produce hormones, drugs, and other biological materials as well as detoxify pollutants on demand. When we as aquaculturists identify the need for a product, that product will be developed.

Fisheries

Water use in the Apalachicola-Chattahoochee-Flint River Basin, Alabama, Florida, and Georgia, 2010, and water-use trends, 1985-2010

The Apalachicola-Chattahoochee-Flint (ACF) River Basin encompasses about 20,230 square miles in parts of Alabama, Florida, and Georgia. Increasing population growth and agricultural production from the 1970s to 2010 has prompted increases in water-resources development and substantially increased water demand in the basin. Since the 1980s, Alabama, Florida, Georgia, and the U.S. Army Corps of Engineers are parties to litigation concerning water management in the ACF River Basin. Estimating the 2010 water use in the ACF River Basin is one aspect of a multipart water resources study on the ACF River Basin that began in 2011. This ACF River Basin study is one focus area of the U.S. Geological Survey&rsquo;s National Water Census program. The 2010 water-use estimates for the ACF River Basin are presented in this report. These estimates include an inventory of the quantity and sources of water withdrawn by category of use and location (State and river basin), and the surface-water returns in the ACF River Basin during 2010. Water-use trends from 1985 to 2010 in the basin also are presented. Offstream water-withdrawal data in the ACF River Basin are presented for each of the following categories: public supply, self-supplied domestic, self-supplied commercial, industrial, mining, agricultural (including crop irrigation, livestock, and aquaculture uses), and thermoelectric-power generation. Water-use data are compiled for the 14 subbasins in the ACF River Basin. For the counties in Alabama, Florida, and Georgia that are partially within the ACF River Basin, data are presented for only that part of the county that lies within the basin. A variety of Federal, State, local, private, and online sources in Alabama, Florida, and Georgia were used to gather surface-water and groundwater withdrawal, surface-water discharges (return flows), and water-use data for the ACF River Basin in 2010. The population in the ACF River Basin was 3.835 million in 2010, a 45-percent increase from the 1990 population of nearly 2.636 million. About 92 percent of the 2010 ACF population resided in Georgia with nearly 75 percent living in the Atlanta metropolitan area. In 2010, 1,645 million gallons per day (Mgal/d) of water were withdrawn from groundwater (576 Mgal/d) and surface-water (1,069 Mgal/d) sources in the ACF River Basin. About 89 percent of the groundwater and 83 percent of the surface-water withdrawals were from Georgia. About 5.6 percent of the total groundwater and nearly 4 percent of the total surface-water withdrawals in the ACF River Basin were from Florida, whereas about 5.3 percent of groundwater and nearly 16 percent of surface water were withdrawn in Alabama. Total water use (withdrawals plus public-supplied deliveries) in the ACF River Basin was 1,593 Mgal/d in 2010. About 56 Mgal/d of water withdrawn in the ACF River Basin was delivered (interbasin transfer) to basins beyond the ACF River Basin. About 564 Mgal/d of water was returned to surface-water bodies in the ACF River Basin. Most of that amount, 63 percent, was treated wastewater discharged by public wastewater-treatment facilities. Water used for once-through cooling by thermoelectric-power facilities accounted for nearly 24 percent of the surface-water returns in the basin. About 70 percent of all water withdrawals in the ACF River Basin were by self-supplied agricultural water users and public water suppliers. Agricultural withdrawals were greatest in the Flint River Basin (501 Mgal/d) with ground-water representing 84 percent of the withdrawals from that basin. Within the Flint River Basin, agricultural withdrawals were greatest in the Lower Flint River and Spring Creek subbasins. About 3.52 million people were served by public water suppliers in the ACF River Basin during 2010, and 88 percent of that population used surface water. Georgia had the largest public-supplied population, representing nearly 93 percent (3.17 million) of the public-supplied population in the ACF River Basin. Public water suppliers served 193,700 people (5.7 percent) in Alabama and 31,880 people in Florida (1.3 percent). Public-supply losses were estimated at 101 Mgal/d. Withdrawals for public supply (483 Mgal/d) and self-supplied industry (141 Mgal/d) were greatest in the Chattahoochee River Basin. Surface water accounted for 96 percent of all withdrawals in the Chattahoochee River Basin. Withdrawals for public supply were greatest in the Upper Chattahoochee River subbasin (366 Mgal/d), whereas self-supplied industrial withdrawals were greatest in the Lower Chattahoochee River subbasin (110 Mgal/d). Water-use trends in the ACF River Basin have varied during the 25 years between 1985 and 2010. Surface-water withdrawals declined between 1985 and 2000, sharply increased in 2000, and declined again between 2000 and 2010. In contrast, groundwater withdrawals increased between 1985 and 2000, declined in 2005, and increased between 2005 and 2010.

Alabama, Florida, Georgia

Water-quality assessment of the upper Snake River Basin, Idaho and western Wyoming — Summary of aquatic biological data for surface water through 1992

The 35,800-square-mile upper Snake River Basin in eastern Idaho and western Wyoming was one of 20 areas selected for water-quality study under the National Water-Quality Assessment Program. As part of the initial phase of the study, data were compiled to describe the current (1992) and historical aquatic biological conditions of surface water in the basin. This description of natural and human environmental factors that affect aquatic life provides the framework for evaluating the status and trends of aquatic biological conditions in streams of the basins. Water resource development and stream alterations, irrigated agriculture, grazing, aquaculture, and species introductions have affected stream biota in the upper Snake River Basin. Cumulative effects of these activities have greatly altered cold-water habitat and aquatic life in the middle Snake River reach (Milner Dam to King Hill). Most of the aquatic Species of Special Concern in the basin , consisting of eight native mollusks and three native fish species, are in this reach of the Snake River. Selected long-term studies, including comprehensive monitoring on Rock Creek, have shown reduced pollutant loadings as a result of implementing practice on cropland; however, aquatic life remains affected by agricultural land use. Community level biological data are lacking for most of the streams in the basin, especially for large river. Aquatic life used to assess water quality of the basin includes primarily macroinvertebrate and fish communities. At least 26 different macroinvertebrate and fish community metrics have been utilized to assess water quality of the basin. Eight species of macroinvertebrates and fish are recognized as Species of Special Concern. The native fish faunas of the basin are composed primarily of cold-water species representing 5 families and 26 species. An additional 13 fish species have been introduced to the basin. Concentrations of synthetic organic compounds and trace-element contaminants in whole fish collected in the basin during 1970-90 generally did not exceed National Academy of Sciences and National Academy of Engineering concentration guidelines or the 1980-81 geometric mean concentrations from samples collected as part of the U.S. Fish and Wildlife Service National Contaminant Biomonitoring Program. Currently, there are no State fish consumption advisories on any streams in the basin, The organochlorine compounds DDT and PCB's were the most frequently detected fish tissue contaminant. Selected long-term data on DDT, its metabolites, and PCB's indicate decreasing concentrations of these compounds. Arsenic, mercury, and selenium were slightly elevated compared with nationwide baseline concentrations and may indicate bioaccumularion in the food chain. Concentrations of most other trace elements in fish tissue were below levels of concerns for the protection of humans and wildlife.

Idaho, Wyoming

Guidance documents: Continued support to improve operations of fish hatcheries and field sites to reduce the impact or prevent establishment of New Zealand Mudsnails and other invasive mollusks

This project tested and revised a risk assessment/management tool authored by Moffitt and Stockton designed to provide hatchery biologists and others a structure to measure risk and provide tools to control, prevent or eliminate invasive New Zealand mudsnails (NZMS) and other invasive mollusks in fish hatcheries and hatchery operations. The document has two parts: the risk assessment tool, and an appendix that summarizes options for control or management. The framework of the guidance document for risk assessment/hatchery tool combines approaches used by the Hazard Analysis and Critical Control Points (HACCP) process with those developed by the Commission for Environmental Cooperation (CEC), of Canada, Mexico, and the United States, in the Tri-National Risk Assessment Guidelines for Aquatic Alien Invasive Species. The framework approach for this attached first document assesses risk potential with two activities: probability of infestation and consequences of infestation. Each activity is treated equally to determine the risk potential. These two activities are divided into seven basic elements that utilize scientific, technical, and other relevant information in the process of the risk assessment. To determine the probability of infestation four steps are used that have scores reported or determined and averaged. This assessment follows a familiar HACCP process to assess pathways of entry, entry potential, colonization potential, spread potential. The economic, environmental and social consequences are considered as economic impact, environmental impact, and social and cultural influences. To test this document, the Principal Investigator worked to identify interested hatchery managers through contacts at regional aquaculture meetings, fish health meetings, and through the network of invasive species managers and scientists participating in the Western Regional Panel on Aquatic Nuisance Species and the 100th Meridian Initiative's Columbia River Basin Team, and the Western New Zealand Mudsnail Conference in Seattle. Targeted hatchery workshops were conducted with staff at Dworshak National Fish Hatchery Complex (ID), Similkameen Pond, Oroville WA, and Ringold Springs State Hatchery (WA). As a result of communications with hatchery staff, invasive species managers, and on site assessments of hatchery facilities, the document was modified and enhanced. Additional resources were added to keep it up to date. The result is a more simplified tool that can lead hatchery or management personnel through the process of risk assessment and provide an introduction to the risk management and communication process. In addition to the typical HACCP processes, this tool adds steps to rate and consider uncertainty and the weight of evidence regarding options and monitoring results . Uncertainty of outcome exists in most tools that can be used to control or prevent NZMS or other invasive mollusks from infesting an area. In additional this document emphasizes that specific control tools and plans must be tailored to each specific setting to consider the economic, environmental and social influences. From the testing and evaluation process, there was a strong recognition that a number of control and prevention tools previously suggested and reported in the literature from laboratory and small scale trials may not be compatible with regional and national regulations, economic constraints, social or cultural constraints, engineering or water chemistry characteristics of each facility. The options for control are summarized in the second document, Review of Control Measures for Hatcheries Infested with NZMS (Appendix A) that provides sources for additional resources and specific tools, and guidance regarding the feasibility and success of each approach. This tool also emphasizes that management plans need to be adaptive and incorporate oversight from professionals familiar with measuring risks of fish diseases, and treatments (e.g. the fish health practitioners and water quality and effluent management teams). Finally, with such a team, the adaptive management approach must be ongoing, and become a regular component of hatchery operations. Although it was the intent that this two part document would be included as part of the revised National Management and Control Plan for the NZMS proposed by the U.S. Fish and Wildlife Service (USFWS) and others, it is provided as a stand-alone document.

Cooperator Science Series