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At least 325 records · Page 18Linked to original sources

Use of real-time PCR to detect canine parvovirus in feces of free-ranging wolves

Using real-time PCR, we tested 15 wolf ( Canis lupus ) feces from the Superior National Forest (SNF), Minnesota, USA, and 191 from Yellowstone National Park (YNP), USA, collected during summer and 13 during winter for canine parvovirus (CPV)-2 DNA. We also tested 20 dog feces for CPV-2 DNA. The PCR assay was 100% sensitive and specific with a minimum detection threshold of 10 4 50% tissue culture infective dose. Virus was detected in two winter specimens but none of the summer specimens. We suggest applying the technique more broadly especially with winter feces.

Minnesota, Wyoming

Serologic and molecular evidence for testudinid herpesvirus 2 infection in wild Agassiz’s desert tortoise, Gopherus agassizii

Following field observations of wild Agassiz’s desert tortoises ( Gopherus agassizii ) with oral lesions similar to those seen in captive tortoises with herpesvirus infection, we measured the prevalence of antibodies to Testudinid herpesvirus (TeHV) 3 in wild populations of desert tortoises in California. The survey revealed 30.9% antibody prevalence. In 2009 and 2010, two wild adult male desert tortoises, with gross lesions consistent with trauma and puncture wounds, respectively, were necropsied. Tortoise 1 was from the central Mojave Desert and tortoise 2 was from the northeastern Mojave Desert. We extracted DNA from the tongue of tortoise 1 and from the tongue and nasal mucosa of tortoise 2. Sequencing of polymerase chain reaction products of the herpesviral DNA-dependent DNA polymerase gene and the UL39 gene respectively showed 100% nucleotide identity with TeHV2, which was previously detected in an ill captive desert tortoise in California. Although several cases of herpesvirus infection have been described in captive desert tortoises, our findings represent the first conclusive molecular evidence of TeHV2 infection in wild desert tortoises. The serologic findings support cross-reactivity between TeHV2 and TeHV3. Further studies to determine the ecology, prevalence, and clinical significance of this virus in tortoise populations are needed.

California;Nevada

Immunization of fishes: A review

Immune response in fishes, which are ectothermic animals, depends on temperature. The optimum response is at the optimum temperature for the species. It is, however, slower in coldwater fishes, such as salmonids, and faster in warmwater fishes. Serum of fishes contains proteins very similar, but not identical, with those of mammals. Immune bodies are contained in gamma, beta, and alpha globulins. Gamma globulin is absent in some fishes. Fishes can be effectively immunized by injection of antigens, however, this is not a practical method. For this reason oral immunization has been attempted repeatedly but the effectiveness is variable.

Journal of Wildlife Diseases

Serum protein changes in immune and nonimmune pigeons infected with various strains of Trichomonas gallinae

Serum protein changes were studied in immune and nonimmune pigeons infected with three different strains of Trichomonas gallinae . Strain I (nonvirulent) produced no change in the relative concentration of serum components. Strains II (oral canker) and III (Jones' Barn) produced decreases in albumin and alpha globulins, and increases in beta and gamma globulins between the 7th and 20th days post infection. Birds infected with strain II began to return to normal by the 20th day, while all those infected with strain III were dead between 10 and 14 days post infection. Two serum protein patterns resulted from infection of immune birds with the Jones' Barn strain. One showed no change in relative protein concentrations and no tissue invasion by the parasite while the other was similar to that seen in nonimmune birds infected with a strain producing oral canker. These also showed evidence of tissue invasion by the parasite. It was concluded that tissue invasion was necessary to evoke a quantitative change in serum protein concentrations.

Journal of Wildlife Diseases

An outbreak of fowl cholera in waterfowl on the Chesapeake Bay

An outbreak of fowl cholera ( Pasteurella multocida infection) occurred in waterfowl wintering on the Chesapeake Bay during February to March 1970. Losses were primarily confined to sea ducks: oldsquaws ( Clangula hyemalis ), white-winged scoters ( Melanitta deglandi ), golden-eyes ( Bucephala clangula ), and buffleheads ( Bucephala albeola ).

Maryland

Avian cholera in cedar waxwings in Ohio

Avian cholera ( Pasteurella multocida infection) was responsible for a localized die-off of cedar waxwings ( Bombycilla cedrorum ) at Steubenville, Ohio in late June, 1968.

Ohio

Some physiologic blood values of wild diving ducks

Blood samples were obtained from 54 canvasbacks ( Aythya valisineria ), 30 lesser scaup ( A. affinis ), 3 ring-necks ( A. collaris ), and 3 buffleheads ( Bucephala albeola ), which were wintering on Chesapeake Bay. These blood samples were used for: red blood cell counts (cans. 2.56 × 10 6 /mm 3 ; scaup 2.45 × 10 6 /mm 3 ; ring-necks 2.50 × 10 6 /mm 3 ; bufflehead 2.64 × 10 6 /mm 3 ), packed cell volume (cans. 52.2%; scaup 57.1%; ring-neck 49.1%; buffleheads 54.3%), total protein (cans. 4.4 g/100 ml; scaup 4.4 g/100 ml; ring-neck 3.5 g/100 ml; bufflehead 3.8 g/100 ml), erythrocyte measurements, and electrophoretic analysis of plasma and serum. Variations between individuals of the same species and sex were slight but a possible difference between male and female erythrocyte numbers and packed cell volumes was evident in the canvasback and scaup samples.

Maryland