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Effects of flood control alternatives on fish and wildlife resources of the Malheur-Harney lakes basin

Malheur Lake is the largest freshwater marsh in the western contiguous United States and is one of the main management units of the Malheur National Wildlife Refuge in southeastern Oregon. The marsh provides excellent waterfowl production habitat as well as vital migration habitats for birds in the Pacific flyway. Water shortages have typically been a problem in this semiarid area; however, record snowfalls and cool summers have recently caused Malheur Lake to rise to its highest level in recorded history. This has resulted in the loss of approximately 57,000 acres of important wildlife habitat as well as extensive flooding of local ranches, roads, and railroad lines. Because of the importance of the Refuge, any water management plan for the Malheur-Harney Lakes Basin needs to consider the impact of management alternatives on the hydrology of Malheur Lake. The facilitated modeling workshop described in this report was conducted January 14-18, 1985, under the joint sponsorship of the Portland Ecological Services Field Office and the Malheur National Wildlife Refuge, Region 1, U.S. Fish and Wildlife Service (FWS). The Portland Field Office is responsible for FWS reporting requirements on Federal water resource projects while the Refuge staff has management responsibility for much of the land affected by high water levels in the Malheur-Harney Lakes Basin. The primary objective of the workshop was to begin gathering and analyzing information concerning potential fish and wildlife impacts, needs, and opportunities associated with proposed U.S. Army Corps of Engineers (COE) flood control alternatives for Malheur Lake. The workshop was structured around the formulation of a computer model that would simulate the hydrologic effects of the various alternatives and any concommitant changes in vegetation communities and wildlife use patterns. The simulation model is composed of three connected submodels. The Hydrology submodel calculates changes in lake volume, elevation, and surface area, as well as changes in water quality, that result from the proposed water management projects (upstream storage, upstream diversions, drainage canals) and the no action alternative. The Vegetation submodel determines associated changes in the areal extent of wetland and upland vegetation communities. Finally, the Wildlife submodel calculates indices of abundance or habitat suitability for colonial nesting birds (great egret, double-crested cormorant, white-faced ibis), greater sandhill crane, diving ducks, tundra swan, dabbling ducks, and Canada goose based on hydrologic and vegetation conditions. The model represents the Malheur-Harney Lakes Basin, but provides water quantity and quality indicators associated with additional flows that might occur in the Malheur River Basin. Several management scenarios, representing various flood control alternatives and assumptions concerning future runoff, were run to analyze model behavior. Scenario results are not intended as an analysis of all potential management actions or assumptions concerning future runoff. Rather, they demonstrate the type of analysis that could be conducted if the model was sufficiently refined and tested. Early in a model development project, the process of building the model is usually of greater benefit than the model itself. The model building process stimulates interaction among agencies, assists in integrating existing information, and helps identify research needs. These benefits usually accrue even in the absence of real predictive power in the resulting model. This workshop initiated interaction among the primary State and Federal resource and development agencies in a nonadversarial forum. The exchange of information and expertise among agencies provided the FWS with the best information currently available for use in the Planning Aid Letter it will develop at the Reconnaissance state of the COE study. If the COE subsequently initiates a Feasability Study, this information will be refined further and will aid the FWS in preparing its Coordination Act Report on any flood control alternative proposed by the COE. The model building and testing process also helped identify model limitations and more general information needs that should be evaluated for further study prior to preparation of an FWS Coordination Act Report. Major needs associated with the Hydrology submodel include a more detailed representation of hydrologic units (separately consider Harney Lake, Mud Lake, and Malheur Lake or the three hydrological units within Malheur Lake, rather than a combined lake system) and explicitly representation of groundwater storage and discharge in water budget calculations. A better representation of the hydrological units will require more detailed topographic data for the basin, capacity-elevation and elevation-surface area curves for each unit, and better water flow data between the units. Additional water quality parameters and constraints on proposed canal operation due to conditions in the Malheur River might also be added. Key Vegetation submodel needs include fine-tuning existing vegetation relationships in the model and adding relationships to address the influence of historical conditions on vegetation development, effects of very rapid changes in lake level, effects of wildlife populations (e.g., carp, muskrat), responses of vegetation to habitat management actions (e.g, haying, grazing, burning), and better representation of sago pondweed dynamics. A complementary geographic information system might also be developed for spatial analyses. Major needs that should be evaluated for the Wildlife submodel include addition of other wildlife species that have important effects on habitat on the Refuge (e.g., carp, muskrat) and consideration of additional life-cycle requisites and controlling variable for species presently in the model. Some of these limitations could perhaps be overcome if historical data on habitat conditions were developed to use with historical data on wildlife populations.

Oregon

Changes in fish diets and food web mercury bioaccumulation induced by an invasive planktivorous fish

The invasion, boom, collapse, and reestablishment of a population of the planktivorous threadfin shad in Clear Lake, California, USA, were documented over a 20-year period, as were the effects of changing shad populations on diet and mercury (Hg) bioaccumulation in nearshore fishes. Threadfin shad competitively displaced other planktivorous fish in the lake, such as inland silversides, young-of-year (YOY) largemouth bass, and YOY bluegill, by reducing zooplankton abundance. As a result, all three species shifted from a diet that was dominated by zooplankton to one that was almost entirely zoobenthos. Stable carbon isotopes corroborated this pattern with each species becoming enriched in δ 13 C, which is elevated in benthic vs. pelagic organisms. Concomitant with these changes, Hg concentrations increased by ∼50% in all three species. In contrast, obligate benthivores such as prickly sculpin showed no relationship between diet or δ 13 C and the presence of threadfin shad, suggesting that effects of the shad were not strongly linked to the benthic fish community. There were also no changes in Hg concentrations of prickly sculpin. The temporary extirpation of threadfin shad from the lake resulted in zooplankton densities, foraging patterns, isotope ratios, and Hg concentrations in pelagic fishes returning to pre-shad values. These results indicate that even transient perturbations of the structure of freshwater food webs can result in significant alterations in the bioaccumulation of Hg and that food webs in lakes can be highly resilient.

Ecological Applications

Coastal habitat and biological community response to dam removal on the Elwha River

Habitat diversity and heterogeneity play a fundamental role in structuring ecological communities. Dam emplacement and removal can fundamentally alter habitat characteristics, which in turn can affect associated biological communities. Beginning in the early 1900s, the Elwha and Glines Canyon dams in Washington, USA, withheld an estimated 30 million tonnes of sediment from river, coastal, and nearshore habitats. During the staged removal of these dams—the largest dam removal project in history—over 14 million tonnes of sediment were released from the former reservoirs. Our interdisciplinary study in coastal habitats—the first of its kind—shows how the physical changes to the river delta and estuary habitats during dam removal were linked to responses in biological communities. Sediment released during dam removal resulted in over a meter of sedimentation in the estuary and over 400 m of expansion of the river mouth delta landform. These changes increased the amount of supratidal and intertidal habitat, but also reduced the influx of seawater into the pre-removal estuary complex. The effects of these geomorphic and hydrologic changes cascaded to biological systems, reducing the abundance of macroinvertebrates and fish in the estuary and shifting community composition from brackish to freshwater-dominated species. Vegetation did not significantly change on the delta, but pioneer vegetation increased during dam removal, coinciding with the addition of newly available habitat. Understanding how coastal habitats respond to large-scale human stressors—and in some cases the removal of those stressors—is increasingly important as human uses and restoration activities increase in these habitats.

Washington

Diel and seasonal variation in food habits of Atlantic salmon parr in a small stream

The diel and seasonal food habits of young-of-year (YOY) and post-young-of-year (PYOY) Atlantic salmon ( Salmo salar ) parr were assayed over the course of 11 months in the West Brook, Massachusetts USA. Gut fullness of YOY salmon did not vary significantly among months. PYOY salmon exhibited significant seasonal differences in gut fullness, with peak fullness occurring in the spring and late fall. Significant diel differences in PYOY gut fullness occurred in June and April, with peak fullness always occurring at dawn. Prey composition varied substantially among months. Dominant prey items of PYOY salmon were baetid mayflies in June, July, and August, limnephilid caddisflies in October and November, and ephemerellid mayflies in February and April. Few differences in prey composition between PYOY and YOY salmon were observed. Fish growth was unrelated to prey availability, but gut fullness explained up to 97% of growth variation across seasons. Results suggest that spring and fall are critical periods of feeding for PYOY salmon and that diel feeding intensity shifts seasonally.

Journal of Freshwater Ecology

Modeling seasonal dynamics of small fish cohorts in fluctuating freshwater marsh landscapes

Small-bodied fishes constitute an important assemblage in many wetlands. In wetlands that dry periodically except for small permanent waterbodies, these fishes are quick to respond to change and can undergo large fluctuations in numbers and biomasses. An important aspect of landscapes that are mixtures of marsh and permanent waterbodies is that high rates of biomass production occur in the marshes during flooding phases, while the permanent waterbodies serve as refuges for many biotic components during the dry phases. The temporal and spatial dynamics of the small fishes are ecologically important, as these fishes provide a crucial food base for higher trophic levels, such as wading birds. We develop a simple model that is analytically tractable, describing the main processes of the spatio-temporal dynamics of a population of small-bodied fish in a seasonal wetland environment, consisting of marsh and permanent waterbodies. The population expands into newly flooded areas during the wet season and contracts during declining water levels in the dry season. If the marsh dries completely during these times (a drydown), the fish need refuge in permanent waterbodies. At least three new and general conclusions arise from the model: (1) there is an optimal rate at which fish should expand into a newly flooding area to maximize population production; (2) there is also a fluctuation amplitude of water level that maximizes fish production, and (3) there is an upper limit on the number of fish that can reach a permanent waterbody during a drydown, no matter how large the marsh surface area is that drains into the waterbody. Because water levels can be manipulated in many wetlands, it is useful to have an understanding of the role of these fluctuations.

Landscape Ecology

A mosaic-based approach is needed to conserve biodiversity in disturbed freshwater ecosystems

Conserving native biodiversity in the face of human‐ and climate‐related impacts is a challenging and globally important ecological problem that requires an understanding of spatially connected, organismal‐habitat relationships. Globally, a suite of disturbances (e.g., agriculture, urbanization, climate change) degrades habitats and threatens biodiversity. A mosaic approach (in which connected, interacting collections of juxtaposed habitat patches are examined) provides a scientific foundation for addressing many disturbance‐related, ecologically based conservation problems. For example, if specific habitat types disproportionately increase biodiversity, these keystones should be incorporated into research and management plans. Our sampling of fish biodiversity and aquatic habitat along ten 3‐km sites within the Upper Neosho River subdrainage, KS, from June‐August 2013 yielded three generalizable ecological insights. First, specific types of mesohabitat patches (i.e., pool, riffle, run, and glide) were physically distinct and created unique mosaics of mesohabitats that varied across sites. Second, species richness was higher in riffle mesohabitats when mesohabitat size reflected field availability. Furthermore, habitat mosaics that included more riffles had greater habitat diversity and more fish species. Thus, riffles (<5% of sampled area) acted as keystone habitats. Third, additional conceptual development, which we initiate here, can broaden the identification of keystone habitats across ecosystems and further operationalize this concept for research and conservation. Thus, adopting a mosaic approach can increase scientific understanding of organismal‐habitat relationships, maintain natural biodiversity, advance spatial ecology, and facilitate effective conservation of native biodiversity in human‐altered ecosystems.

Kansas

Egg size scales negatively with system size in a periodic fish species

Optimal egg size theory implies that female organisms balance between fecundity and individual offspring investment according to their environment. Past interspecific studies suggest that fishes in large marine systems generally produce smaller eggs than those in small freshwater systems. We tested whether intraspecific egg size variation reflected a similar pattern by comparing egg size among yellow perch ( Perca flavescens ) populations inhabiting a range of system sizes. In 2018, 2019, and 2023, we collected yellow perch egg samples from 12 locations in systems ranging in surface area from 37 to 5,390,492 ha. First, we found that egg diameter significantly increased with maternal total length in five of eight individually tested populations. After accounting for these maternal effects, we found a significant interaction, where females inhabiting larger lakes, such as the main basins of Lakes Erie and Michigan, produced smaller eggs than those in smaller inland lakes, and the greatest differences were demonstrated among females of greater total length. This egg size variation in the largest females is consistent with interspecific egg size comparisons between marine and freshwater fishes. However, by examining a single species across vastly different environments, we were able to support theoretical expectations that maternal investment in offspring should vary with environmental conditions controlling early-life resource acquisition and competition.

Ecology and Evolution

Diel periodicity of drift of larval fishes in tributaries of Lake Ontario

Diel patterns of downstream drift were examined during mid-June in three tributaries of Lake Ontario. Larval fishes were collected in drift nets that were set in each stream for 72 consecutive hours and emptied at 4-h intervals. Fantail darter (Ethostoma flabellare) and blacknose dace (Rhinichthys atractulus) were the two most abundant native stream fishes and were two of the three species collected in the ichthyoplankton drift. Fantail darter larvae comprised 100%, 98.9%, and 70.2% of the ichthyoplankton in the three streams. Most larval fishes (96%) drifted at night with peak catches occurring at 2400h in Orwell Brook and Trout Brook and 0400h in Little Sandy Creek. Based on stream temperatures, peak spawning and larval drift of blacknose dace probably occurred later in the season.

Journal of Freshwater Ecology

Climate, invasive species and land use drive population dynamics of a cold-water specialist

Climate change is an additional stressor in a complex suite of threats facing freshwater biodiversity, particularly for cold-water fishes. Research addressing the consequences of climate change on cold-water fish has generally focused on temperature limits defining spatial distributions, largely ignoring how climatic variation influences population dynamics in the context of other existing stressors. We used long-term data from 92 populations of bull trout Salvelinus confluentus – one of North America's most cold-adapted fishes – to quantify additive and interactive effects of climate, invasive species and land use on population dynamics (abundance, variability and growth rate). Populations were generally depressed, more variable and declining where spawning and rearing stream habitat was limited, invasive species and land use were prevalent and stream temperatures were highest. Increasing stream temperature acted additively and independently, whereas land use and invasive species had additive and interactive effects (i.e. the impact of one stressor depended on exposure to the other stressor). Most (58%–78%) of the explained variation in population dynamics was attributed to the presence of invasive species, differences in life history and management actions in foraging habitats in rivers, lakes and reservoirs. Although invasive fishes had strong negative effects on populations in foraging habitats, proactive control programmes appeared to effectively temper their negative impact. Synthesis and applications . Long-term demographic data emphasize that climate warming will exacerbate imperilment of cold-water specialists like bull trout, yet other stressors – especially invasive fishes – are immediate threats that can be addressed by proactive management actions. Therefore, climate-adaptation strategies for freshwater biodiversity should consider existing abiotic and biotic stressors, some of which provide potential and realized opportunity for conservation of freshwater biodiversity in a warming world.

Journal of Applied Ecology

Size of age-0 crappies ( Pomoxis spp.) relative to reservoir habitats and water levels

Variable year-class strength is common in crappie Pomoxis spp. populations in many reservoirs, yet the mechanisms behind this variability are poorly understood. Size-dependent mortality of age-0 fishes has long been recognized in the population ecology literature; however, investigations about the effects of environmental factors on age-0 crappie size are lacking. The objective of this study was to determine if differences existed in total length of age-0 crappies between embayment and floodplain habitats in reservoirs, while accounting for potential confounding effects of water level and crappie species. To this end, we examined size of age-0 crappies in four flood-control reservoirs in northwest Mississippi over 4years. Age-0 crappies inhabiting uplake floodplain habitats grew to a larger size than fish in downlake embayments, but this trend depended on species, length of time a reservoir was dewatered in the months preceding spawning, and reservoir water level in the months following spawning. The results from our study indicate that water-level management may focus not only on allowing access to quality nursery habitat, but that alternating water levels on a multiyear schedule could increase the quality of degraded littoral habitats.

Journal of Freshwater Ecology

Impacts of dams on river herring populations through their native range: What’s left?

Introduction: River herring (alewife, Alosa pseudoharengus , and blueback herring, Alosa aestivalis ) are native along the East Coast of North America. These fish are culturally important and provide critical ecosystem linkages between marine and freshwater habitats. Due to overfishing, and habitat loss (through degradation and damming), river herring populations are at historical lows across their native range. In the last 200 years, dams and other impoundments have reduced access to spawning habitat for these and other anadromous fish. Methods: To assess the theoretical coast-wide spawning potential for river herring, we quantified historically accessible spawning habitat (pre-dam) in coastal freshwater rivers using physical river characteristics (width and gradient). To assess the impact of dams on spawning habitat, we used a dams database to segment river reaches. This allowed us to characterize spawning habitat upstream and downstream of dams. River-specific population models were then used to estimate the number of potential spawners for each species, based on habitat estimates and life history parameters. We investigated three scenarios: no dams, favorable dam passage, and no dam passage. We use these simulations to compare the theoretical spawning potential for river herring prior to and after dam construction coastwide. Results: We estimated that 52% of alewife and 51% of blueback herring habitat is located upstream of dams throughout the East Coast of North America. This results in a theoretical loss of ~880 million alewife and ~100 million blueback herring potential spawners due to dams, reducing both the ecological connection and fishery potential of these species. Discussion: Even with the best-case “current” scenario for fish passage, we observed marginal increases in spawner abundance. These results highlight the relatively small theoretical influence current passage structures may have on restoring river herring to their historical abundances compared to dam removal. Our sensitivity analyses demonstrate that upstream passage to access additional habitat is only advantageous when effective juvenile and adult downstream passage are in place.

East Coast of North America

Factors affecting swimming performance of fasted rainbow trout with implications of exhaustive exercise on overwinter mortality

We evaluated the effects of body size, water temperature, and sustained swimming activity on swimming performance and the effects of exhaustive exercise on mortality of fasted juvenile rainbow trout. Fasting caused swimming performance to decline more rapidly for small fish than large fish, and warmer water temperatures and sustained swimming activity further decreased swimming performance. Exhaustive exercise increased mortality among fasted fish. Our observations suggest that juvenile rainbow trout with little or no food intake during winter can swim for long periods of time with little effect on mortality, but swimming to exhaustion can enhance mortality, especially among the smallest juveniles.

Journal of Freshwater Ecology

Blueprints for riverine cod nest boxes draw from multiple design considerations

Designing aquatic nest boxes is rarely afforded detailed scientific account. Here we provide some historical context for nest boxes used in production of large-bodied fishes of the Australian freshwater cod genus Maccullochella . Our experience with eastern freshwater cod is used as a case study to: (a) convey aspects of the complexity of the nest box design process and to (b) demonstrate the importance of visual literacy in project communication across the variety of contributors to the eco-design process. Specifically, we describe a new, two-variant, triangular nest box design for application in rivers and modifications to a standard stainless steel nest box for hatchery-pond-based spawning of eastern freshwater cod M. ikei . We designed the boxes to test adult preference for single versus double entrance/exits to cavities in hatchery and field environments. An important consideration specific to hatchery production is harvesting demersal, adhesive eggs prior to hatching to minimise fungal infection of eggs and physical loss of larvae, in addition to providing critical first feeding of larvae. In contrast, field nest box design incorporated multiple factors and associated trade-offs related to both internal and external design, ranging from manufacturer capability, material types, cost, transportability, hydrological performance, biodegradability, retrievability, as well as biological and ecological function. Only preliminary findings from field nest box deployments are provided here, and we focus primarily on elements of visual language in the form of conceptual drawings, sketches and final schematics which have been central to our process. We emphasise the benefit of harnessing input from multiple fields of expertise and documenting and testing designs of nest boxes for cavity nesting fishes, under both controlled hatchery and more complex field conditions.

Aquaculture, Fish and Fisheries

Modeling diverse environmental responses of reservoirs to floating photovoltaic systems

Floating photovoltaic (FPV) systems are emerging as a promising strategy for large-scale clean energy production worldwide. However, by altering key physical drivers such as solar radiation and wind mixing, FPV installations may have also unintended consequences for lakes and reservoirs. Given the wide diversity of freshwater systems globally, understanding the consistency in direction and magnitude of environmental responses to FPV deployment is critical for informed regulatory oversight and sustainable energy development. Here, we used process-based models to simulate the effects of FPV coverage on 11 reservoirs across the United States. This is the first multi-reservoir analysis using a laterally averaged 2D process-based modeling framework to systematically evaluate FPV impacts across diverse climatic and morphometric contexts, enabling direct comparison of magnitude and direction of responses among systems. Specifically, we evaluated changes in (1) surface and outflow temperature, (2) thermocline depth, (3) water column stability, (4) dissolved oxygen concentrations, and (5) potential suitable habitat availability for warm- and cold-water fishes. We quantified changes in these response variables by an iterative approach that simulates increases in FPV coverage and compares them with reference conditions. We summarized responses for winter (January–February) and summer (July–August). As expected, our simulations show that increasing FPV coverage consistently cooled surface waters and altered thermal stratification patterns, but the magnitude and environmental implications of these changes varied among reservoirs. Notably, greater FPV coverage led to increased variability in habitat suitability for aquatic species, with some reservoirs exhibiting distinct and sometimes divergent responses. These findings underscore the importance of considering local environmental contexts when assessing FPV impacts. While large-scale FPV systems offer potential benefits for climate mitigation, their ecological effects, particularly on thermally sensitive biota, require careful site-specific evaluation to avoid unintended consequences to local freshwater biodiversity.

Arkansas, Idaho, Ohio, Oregon, Tennessee, Washingt

Managing climate refugia for freshwater fishes under an expanding human footprint

Within the context of climate adaptation, the concept of climate refugia has emerged as a framework for addressing future threats to freshwater fish populations. We evaluated recent climate‐refugia management associated with water use and landscape modification by comparing efforts in the US states of Oregon and Massachusetts, for which there are contrasting resource use patterns. Using these examples, we discuss tools and principles that can be applied more broadly. Although many early efforts to identify climate refugia have focused on water temperature, substantial gains in evaluating other factors and processes regulating climate refugia (eg stream flow, groundwater availability) are facilitating refined mapping of refugia and assessment of their ecological value. Major challenges remain for incorporating climate refugia into water‐quality standards, evaluating trade‐offs among policy options, addressing multiple species’ needs, and planning for uncertainty. However, with a procedurally transparent and conceptually sound framework to build upon, recent efforts have revealed a promising path forward.

Massachusetts, Oregon

Effects of episodic stream dewatering on brook trout spatial population structure

Stream dewatering is expected to become more prevalent due to climate change, and we explored the potential consequences for brook trout ( Salvelinus fontinalis) within a temperate forest ecosystem in eastern North America. We estimated fish density within stream pools ( n = 386) from electrofishing surveys over 10 years (2012–2021) to compare a stream that exhibits episodic dewatering (Paine Run) against a stream of similar size that remains flow-connected (Staunton River) within Shenandoah National Park, Virginia (U.S.A.). Annual surveys encompassed fluvial distances ranging from 2.6 to 4.4 km in each stream. Mean annual fish density (fish/pool m 2 ) was not different between streams for juvenile or adult age classes, but spatial variation in density was greater in Paine Run for both age classes of fish. Paine Run also included a greater proportion of unoccupied pools than Staunton River and exhibited stronger spatial autocorrelation in fish density among nearby pools, suggesting dispersal limitation due to surface flow fragmentation. Fish density in pools increased during years with low summer precipitation, and this effect was observed in both streams but was stronger in Paine Run than Staunton River, further indicating the importance of fish movement into pools in response to low-flow thresholds. Our results indicate the importance of pools as ecological refuges during low-flow conditions and that episodic dewatering may affect extirpation risks for brook trout by sequestering more fish into fewer areas. Our findings also highlight the importance of hydrological variation within stream networks because downstream river gages could not predict the observed spatial heterogeneity in fish density or pool occupancy.

Freshwater Biology

Species traits predict stream-fish invaders in an Appalachian (U.S.A.) river basin

We compared the influence of biological traits (morphology, physiology, reproduction, and life history), ecological traits (geographic distribution, habitat associations, food habits), and introduction attributes (propagule pressure, human use of a species, residence time) on invasion success of native and introduced stream fishes during the colonisation and spread stages in an Appalachian (U.S.A.) river basin. Colonisation success was positively related to residence time, benthic feeding, an equilibrium life-history strategy, and nest spawning. Successful spread was associated with tolerance to increased temperature and an equilibrium life-history strategy. The spread of introduced fishes was negatively related to gamefish status. No effect of propagule pressure was detected. Traits linked to invasion success were consistent with the hypothesis that human land-use practices increase the invasibility of highland catchments by creating novel conditions better suited to lowland and equilibrium invaders. We found biological traits to be more useful than ecological traits in predicting invasion success and suggesting invasion mechanisms. Parental care and nest association can facilitate invasions over large spatial extents for both native and introduced fishes. Analyses of suites of traits can reveal mechanisms of invasions and tactics for controlling them; such mechanisms and tactics may be system-specific and scale-dependent.

North Carolina, Virginia

Development and assessment of fluorescent-dyed, preserved invasive grass carp (Ctenopharyngodon idella) eggs as surrogates for live eggs in transport and dispersal control experiments

Invasive species such as grass carp ( Ctenopharyngodon idella ) pose substantial ecological threats to North American freshwater ecosystems. Understanding their early life stage behavior is critical for management efforts. From spawning to hatching, invasive carp eggs must remain suspended in the water column while drifting downstream for the best chance of survival. This highly vulnerable life stage is a potential target for population control to reduce recruitment. However, studying egg transport and potential dispersal control techniques is challenging, because the availability of live eggs and time period for experimentation are extremely limited. Additionally, accurately replicating the physical characteristics and transport mechanisms of fish eggs using surrogates in laboratory and field studies is not trivial. This study presents a novel method to create fluorescein-dyed, preserved grass carp eggs as surrogates for live eggs in transport and dispersal control experiments. This technique enables year-round studies of grass carp egg transport, offering managers a reliable tool for developing and testing dispersal control and passive sampling methods for invasive carp eggs. In this study, we rehydrate and dye preserved grass carp eggs in varying concentrations of aqueous fluorescein for a range of rehydration times, evaluate dye retention and egg visibility under ultraviolet light (UV-A), and measure diameters and settling velocities for comparison with live eggs. Eggs rehydrated in 0.100 g per liter fluorescein for 30 min maintain adequate brightness for up to 40 min in mixed conditions and exhibit mean settling velocities and densities similar to live eggs, making them ideal for laboratory experiments using quantitative imaging techniques.

River Research and Applications