SEARCH · Geology Reports

Results for “Anaerobe”

Search indexed USGS publications on groundwater, aquifers, geologic maps, mineral resources and earthquakes. Explore source records by subject and place.

SourceResearch connectionFrom yearThrough year

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 325 records · Page 18Linked to original sources

USGS1990

Rates of microbial metabolism in deep coastal plain aquifers

Rates of microbial metabolism in deep anaerobic aquifers of the Atlantic coastal plain of South Carolina were investigated by both microbiological and geochemical techniques. Rates of [2- 14 C]acetate and [U- 14 C]glucose oxidation as well as geochemical evidence indicated that metabolic rates were faster in the sandy sediments composing the aquifers than in the clayey sediments of the confining layers. In the sandy aquifer sediments, estimates of the rates of CO 2 production (millimoles of CO 2 per liter per year) based on the oxidation of [2- 14 C] acetate were 9.4 × 10 −3 to 2.4 × 10 −1 for the Black Creek aquifer, 1.1 × 10 −2 for the Middendorf aquifer, and <7 × 10 −5 for the Cape Fear aquifer. These estimates were at least 2 orders of magnitude lower than previously published estimates that were based on the accumulation of CO 2 in laboratory incubations of similar deep subsurface sediments. In contrast, geochemical modeling of groundwater chemistry changes along aquifer flowpaths gave rate estimates that ranged from 10 −4 to 10 −6 mmol of CO 2 per liter per year. The age of these sediments (ca. 80 million years) and their organic carbon content suggest that average rates of CO 2 production could have been no more than 10 −4 mmol per liter per year. Thus, laboratory incubations may greatly overestimate the in situ rates of microbial metabolism in deep subsurface environments. This has important implications for the use of laboratory incubations in attempts to estimate biorestoration capacities of deep aquifers. The rate estimates from geochemical modeling indicate that deep aquifers are among the most oligotrophic aquatic environments in which there is ongoing microbial metabolism.

South Carolina↗

USGS1991

Electron transport in the dissimilatory iron reducer, GS-15

Mechanisms for electron transport to Fe(III) were investigated in GS-15, a novel anaerobic microorganism which can obtain energy for growth hy coupling the complete oxidation of organic acids or aromatic compounds to the reduction of Fe(III) to Fe(II). The results indicate that Fe(III) reduction proceeds through a type b cytochrome and a membrane-bound Fe(III) reductase which is distinct from the nitrate reductase.

Applied and Environmental Microbiology↗

USGS1996

Isolation of Geobacter species from diverse sedimentary environments

In an attempt to better understand the microorganisms responsible for Fe(III) reduction in sedimentary environments, Fe(III)-reducing microorganisms were enriched for and isolated from freshwater aquatic sediments, a pristine deep aquifer, and a petroleum-contaminated shallow aquifer. Enrichments were initiated with acetate or toluene as the electron donor and Fe(III) as the electron acceptor. Isolations were made with acetate or benzoate. Five new strains which could obtain energy for growth by dissimilatory Fe(III) reduction were isolated. All five isolates are gram- negative strict anaerobes which grow with acetate as the electron donor and Fe(III) as the electron acceptor. Analysis of the 16S rRNA sequence of the isolated organisms demonstrated that they all belonged to the genus Geobacter in the delta subdivision of the Proteobacteria . Unlike the type strain, Geobacter metallireducens , three of the five isolates could use H 2 as an electron donor for Fe(III) reduction. The deep subsurface isolate is the first Fe(III) reducer shown to completely oxidize lactate to carbon dioxide, while one of the freshwater sediment isolates is only the second Fe(III) reducer known that can oxidize toluene. The isolation of these organisms demonstrates that Geobacter species are widely distributed in a diversity of sedimentary environments in which Fe(III) reduction is an important process.

Applied and Environmental Microbiology↗

USGS1996

Microbial degradation of hydrochlorofluorocarbons (CHCl2F and CHCl2CF3) in soils and sediments

The ability of microorganisms to degrade trace levels of the hydrochlorofluorocarbons HCFC-21 and HCFC-123 was investigated. Methanotroph- linked oxidation of HCFC-21 was observed in aerobic soils, and anaerobic degradation of HCFC-21 occurred in freshwater and salt marsh sediments. Microbial degradation of HCFC-123 was observed in anoxic freshwater and salt marsh sediments, and the recovery of 1,1,1-trifluoro-2-chloroethane indicated the involvement of reductive dechlorination. No degradation of HCFC-123 was observed in aerobic soils. In same experiments, HCFCs were degraded at low (parts per billion) concentrations, raising the possibility that bacteria in nature remove HCFCs from the atmosphere.

Applied and Environmental Microbiology↗

USGS1998

Microbial diversity in a hydrocarbon- and chlorinated-solvent- contaminated aquifer undergoing intrinsic bioremediation

A culture-independent molecular phylogenetic approach was used to survey constituents of microbial communities associated with an aquifer contaminated with hydrocarbons (mainly jet fuel) and chlorinated solvents undergoing intrinsic bioremediation. Samples were obtained from three redox zones: methanogenic, methanogenic-sulfate reducing, and iron or sulfate reducing. Small-subunit rRNA genes were amplified directly from aquifer material DNA by PCR with universally conserved or Bacteria - or Archaea -specific primers and were cloned. A total of 812 clones were screened by restriction fragment length polymorphisms (RFLP), approximately 50% of which were unique. All RFLP types that occurred more than once in the libraries, as well as many of the unique types, were sequenced. A total of 104 (94 bacterial and 10 archaeal) sequence types were determined. Of the 94 bacterial sequence types, 10 have no phylogenetic association with known taxonomic divisions and are phylogenetically grouped in six novel division level groups (candidate divisions WS1 to WS6); 21 belong to four recently described candidate divisions with no cultivated representatives (OP5, OP8, OP10, and OP11); and 63 are phylogenetically associated with 10 well-recognized divisions. The physiology of two particularly abundant sequence types obtained from the methanogenic zone could be inferred from their phylogenetic association with groups of microorganisms with a consistent phenotype. One of these sequence types is associated with the genus Syntrophus ; Syntrophus spp. produce energy from the anaerobic oxidation of organic acids, with the production of acetate and hydrogen. The organism represented by the other sequence type is closely related to Methanosaeta spp., which are known to be capable of energy generation only through aceticlastic methanogenesis. We hypothesize, therefore, that the terminal step of hydrocarbon degradation in the methanogenic zone of the aquifer is aceticlastic methanogenesis and that the microorganisms represented by these two sequence types occur in syntrophic association.

Applied and Environmental Microbiology↗

USGS1999

Estimation of methanogen biomass via quantitation of coenzyme M

Determination of the role of methanogenic bacteria in an anaerobic ecosystem often requires quantitation of the organisms. Because of the extreme oxygen sensitivity of these organisms and the inherent limitations of cultural techniques, an accurate biomass value is very difficult to obtain. We standardized a simple method for estimating methanogen biomass in a variety of environmental matrices. In this procedure we used the thiol biomarker coenzyme M (CoM) (2-mercaptoethanesulfonic acid), which is known to be present in all methanogenic bacteria. A high-performance liquid chromatography-based method for detecting thiols in pore water (A. Vairavamurthy and M. Mopper, Anal. Chim. Acta 78:363–370, 1990) was modified in order to quantify CoM in pure cultures, sediments, and sewage water samples. The identity of the CoM derivative was verified by using liquid chromatography-mass spectroscopy. The assay was linear for CoM amounts ranging from 2 to 2,000 pmol, and the detection limit was 2 pmol of CoM/ml of sample. CoM was not adsorbed to sediments. The methanogens tested contained an average of 19.5 nmol of CoM/mg of protein and 0.39 ± 0.07 fmol of CoM/cell. Environmental samples contained an average of 0.41 ± 0.17 fmol/cell based on most-probable-number estimates. CoM was extracted by using 1% tri-( N )-butylphosphine in isopropanol. More than 90% of the CoM was recovered from pure cultures and environmental samples. We observed no interference from sediments in the CoM recovery process, and the method could be completed aerobically within 3 h. Freezing sediment samples resulted in 46 to 83% decreases in the amounts of detectable CoM, whereas freezing had no effect on the amounts of CoM determined in pure cultures. The method described here provides a quick and relatively simple way to estimate methanogenic biomass.

Applied and Environmental Microbiology↗

USGS2004

Structural and spectral features of selenium nanospheres produced by Se-respiring bacteria

Certain anaerobic bacteria respire toxic selenium oxyanions and in doing so produce extracellular accumulations of elemental selenium [Se(0)]. We examined three physiologically and phylogenetically diverse species of selenate- and selenite-respiring bacteria, Sulfurospirillum barnesii, Bacillus selenitireducens, and Selenihalanaerobacter shriftii, for the occurrence of this phenomenon. When grown with selenium oxyanions as the electron acceptor, all of these organisms formed extracellular granules consisting of stable, uniform nanospheres (diameter, ???300 nm) of Se(0) having monoclinic crystalline structures. Intracellular packets of Se(0) were also noted. The number of intracellular Se(0) packets could be reduced by first growing cells with nitrate as the electron acceptor and then adding selenite ions to washed suspensions of the nitrate-grown cells. This resulted in the formation of primarily extracellular Se nanospheres. After harvesting and cleansing of cellular debris, we observed large differences in the optical properties (UV-visible absorption and Raman spectra) of purified extracellular nanospheres produced in this manner by the three different bacterial species. The spectral properties in turn differed substantially from those of amorphous Se(0) formed by chemical oxidation of H2Se and of black, vitreous Se(0) formed chemically by reduction of selenite with ascorbate. The microbial synthesis of Se(0) nanospheres results in unique, complex, compacted nanostructural arrangements of Se atoms. These arrangements probably reflect a diversity of enzymes involved in the dissimilatory reduction that are subtly different in different microbes. Remarkably, these conditions cannot be achieved by current methods of chemical synthesis.

Applied and Environmental Microbiology↗

USGS2004

Redox transformations of arsenic oxyanions in periphyton communities

Periphyton ( Cladophora sp.) samples from a suburban stream lacking detectable dissolved As were able to reduce added As(V) to As(III) when incubated under anoxic conditions and, conversely, oxidized added As(III) to As(V) with aerobic incubation. Both types of activity were abolished in autoclaved controls, thereby demonstrating its biological nature. The reduction of As(V) was inhibited by chloramphenicol, indicating that it required the synthesis of new protein. Nitrate also inhibited As(V) reduction, primarily because it served as a preferred electron acceptor to which the periphyton community was already adapted. However, part of the inhibition was also caused by microbial reoxidation of As(III) linked to nitrate. Addition of [ 14 C]glucose to anoxic samples resulted in the production of 14 CO 2 , suggesting that the observed As(V) reduction was a respiratory process coupled to the oxidation of organic matter. The population density of As(V)-reducing bacteria within the periphyton increased with time and with the amount of As(V) added, reaching values as high as ∼10 6 cells ml −1 at the end of the incubation. This indicated that dissimilatory As(V) reduction in these populations was linked to growth. However, As(V)-respiring bacteria were found to be present, albeit at lower numbers (∼10 2 ml −1 ), in freshly sampled periphyton. These results demonstrate the presence of a bacterial population within the periphyton communities that is capable of two key arsenic redox transformations that were previously studied in As-contaminated environments, which suggests that these processes are widely distributed in nature. This assumption was reinforced by experiments with estuarine samples of Cladophora sericea in which we detected a similar capacity for anaerobic As(V) reduction and aerobic As(III) oxidation.

Applied and Environmental Microbiology↗

USGS2005

Chloroethene biodegradation in sediments at 4°C

Microbial reductive dechlorination of [1,2- 14 C]trichloroethene to [ 14 C] cis -dichloroethene and [ 14 C]vinyl chloride was observed at 4°C in anoxic microcosms prepared with cold temperature-adapted aquifer and river sediments from Alaska. Microbial anaerobic oxidation of [1,2- 14 C] cis -dichloroethene and [1,2- 14 C]vinyl chloride to 14 CO 2 also was observed under these conditions.

Applied and Environmental Microbiology↗

USGS2010

Identification of a novel arsenite oxidase gene, arxA, in the haloalkaliphilic, arsenite-oxidizing bacterium alkalilimnicola ehrlichii strain MLHE-1

Although arsenic is highly toxic to most organisms, certain prokaryotes are known to grow on and respire toxic metalloids of arsenic (i.e., arsenate and arsenite). Two enzymes are known to be required for this arsenic-based metabolism: (i) the arsenate respiratory reductase (ArrA) and (ii) arsenite oxidase (AoxB). Both catalytic enzymes contain molybdopterin cofactors and form distinct phylogenetic clades (ArrA and AoxB) within the dimethyl sulfoxide (DMSO) reductase family of enzymes. Here we report on the genetic identification of a “new” type of arsenite oxidase that fills a phylogenetic gap between the ArrA and AoxB clades of arsenic metabolic enzymes. This “new” arsenite oxidase is referred to as ArxA and was identified in the genome sequence of the Mono Lake isolate Alkalilimnicola ehrlichii MLHE-1, a chemolithoautotroph that can couple arsenite oxidation to nitrate reduction. A genetic system was developed for MLHE-1 and used to show that arxA (gene locus ID mlg _ 0216 ) was required for chemoautotrophic arsenite oxidation. Transcription analysis also showed that mlg _ 0216 was only expressed under anaerobic conditions in the presence of arsenite. The mlg _ 0216 gene is referred to as arxA because of its greater homology to arrA relative to aoxB and previous reports that implicated Mlg_0216 (ArxA) of MLHE-1 in reversible arsenite oxidation and arsenate reduction in vitro . Our results and past observations support the position that ArxA is a distinct clade within the DMSO reductase family of proteins. These results raise further questions about the evolutionary relationships between arsenite oxidases (AoxB) and arsenate respiratory reductases (ArrA). Arsenic is toxic to most organisms and is known to cause cancer in humans. However, bacteria have adapted several biotransformation pathways that function to either couple the reduction or oxidation of arsenicals to energy conservation and growth (1). The enzymologies of these two pathways have several features in common. The arsenate respiratory reductase (ArrAB) and arsenite oxidase (AoxAB) enzymes are usually composed of at least two subunits, a small iron-sulfur cluster-containing subunit (ArrB and AoxA) and a larger molybdopterin-containing catalytic subunit (ArrA and AoxB). Although they catalyze arsenic redox chemistry, ArrA and AoxB form distinct phylogenetic clades within the dimethyl sulfoxide (DMSO) reductase family of molybdenum-containing enzymes (16, 24). Culture-dependent approaches have resulted in the isolation of a variety of diverse bacteria that metabolize arsenic (reviewed in reference 26). Many of these isolates have had their genomes sequenced, which has been insightful for understanding the composition and diversity of arr and aox gene clusters. In the arsenite-oxidizing nitrate reducer Alkalilimnicola ehrlichii strain MLHE-1 (a haloalkaliphile isolated from Mono Lake [CA]) (10, 15), bioinformatic analysis of its genome revealed the absence of genes homologous to the arsenite oxidase genes of the aoxB type. Instead, two genes ( mlg _ 0216 and mlg _ 2426 ) were identified that better resembled the catalytic subunit of the arsenate respiratory reductase (20); however, MLHE-1 has not been shown to respire (or reduce) arsenate (15). Recent work by Richey et al. (20) showed that the Mlg_0216 protein (and not Mlg_2426) was expressed under chemolithoautotrophic (10 mM arsenite and 10 mM nitrate) growth conditions. Moreover, it was shown that Mlg_0216 exhibits both arsenate reductase and arsenite oxidase activities in vitro . These observations raised the question, is the mlg _ 0216 gene required for arsenite oxidation in vivo ? In this report, we addressed this question by developing a genetic system in MLHE-1, generating strains with mutations in mlg _ 0216 and mlg _ 2426 , and physiologically characterizing the resulting strains. Our results implicate mlg _ 0216 in chemolithoautotrophic arsenite oxidation coupled to nitrate respiration.

Journal of Bacteriology↗

USGS1996

Phylogenetic analysis of dissimilatory Fe(III)-reducing bacteria

Evolutionary relationships among strictly anaerobic dissimilatory Fe(III)-reducing bacteria obtained from a diversity of sedimentary environments were examined by phylogenetic analysis of 16S rRNA gene sequences. Members of the genera Geobacter, Desulfuromonas, Pelobacter, and Desulfuromusa formed a monophyletic group within the delta subdivision of the class Proteobacteria. On the basis of their common ancestry and the shared ability to reduce Fe(III) and/or S0, we propose that this group be considered a single family, Geobacteraceae. Bootstrap analysis, characteristic nucleotides, and higher-order secondary structures support the division of Geobacteraceae into two subgroups, designated the Geobacter and Desulfuromonas clusters. The genus Desulfuromusa and Pelobacter acidigallici make up a distinct branch within the Desulfuromonas cluster. Several members of the family Geobacteraceae, none of which reduce sulfate, were found to contain the target sequences of probes that have been previously used to define the distribution of sulfate-reducing bacteria and sulfate-reducing bacterium-like microorganisms. The recent isolations of Fe(III)-reducing microorganisms distributed throughout the domain Bacteria suggest that development of 16S rRNA probes that would specifically target all Fe(III) reducers may not be feasible. However, all of the evidence suggests that if a 16S rRNA sequence falls within the family Geobacteraceae, then the organism has the capacity for Fe(III) reduction. The suggestion, based on geological evidence, that Fe(III) reduction was the first globally significant process for oxidizing organic matter back to carbon dioxide is consistent with the finding that acetate-oxidizing Fe(III) reducers are phylogenetically diverse.

Journal of Bacteriology↗

USGS2021

Acetylene-fueled trichloroethene reductive dechlorination in a groundwater enrichment culture

In aquifers, acetylene (C 2 H 2 ) is a product of abiotic degradation of trichloroethene (TCE) catalyzed by in situ minerals. C 2 H 2 can, in turn, inhibit multiple microbial processes including TCE dechlorination and metabolisms that commonly support dechlorination, in addition to supporting the growth of acetylenotrophic microorganisms. Previously, C 2 H 2 was shown to support TCE reductive dechlorination in synthetic, laboratory-constructed cocultures containing the acetylenotroph Pelobacter sp. strain SFB93 and Dehalococcoides mccartyi strain 195 or strain BAV1. In this study, we demonstrate TCE and perchloroethene (PCE) reductive dechlorination by a microbial community enriched from contaminated groundwater and amended with C 2 H 2 as the sole electron donor and organic carbon source. The metagenome of the stable, enriched community was analyzed to elucidate putative community functions. A novel anaerobic acetylenotroph in the phylum Actinobacteria was identified using metagenomic analysis. These results demonstrate that the coupling of acetylenotrophy and reductive dechlorination can occur in the environment with native bacteria and broaden our understanding of biotransformation at contaminated sites containing both TCE and C 2 H 2 .

mBio↗

USGS1966

Tropical lakes, copropel, and oil shale

During a long-continued study of the lacustrine beds of the Eocene Green River Formation, I have tried to interpret past events from observation of present-day processes. After a search of some 40 years, four lakes have been found that are producing a kind of organic ooze judged to be a modern analogue of the precursors of rich oil shale. Two of the lakes are in central Africa and two are in Florida. All four are shallow. The ooze in all four is predominantly algal, entirely in the form of minute fecal pellets, and does not decay in warm, wet, oxidizing environments. Several of the most unusual, mummified microorganisms found in the oil shale of the Green River are illustrated as testament to the inference that the Eocene organic oozes also were resistant to decay. Studies to determine why these algal oozes do not decay are in progress, but as yet no satisfactory explanation is available. The ooze from Mud Lake, Florida, contains very few living bacteria but a great many bacterial spores, suggesting some active inhibitor. Gentle and slow anaerobic decay takes place in the ooze 1 foot or more below the mud-water interface. The algal ooze accumulates slowly. That at a depth of 3 feet below the mud-water interface has a C 14 age of 2280 ± 200 years. If compacted, this 3-foot layer would amount to a layer only about 0.5 inch thick. The air-dried algal mud (from Mud Lake) looks much like oil shale and has a C-H ratio essentially like that of the organic matter in oil shale. The oxygen of the mud, however, is roughly 5 times as high as in oil shale. The calorific value of dried Mud Lake algal ooze is about 6600 cal/g, whereas the organic matter from Green River oil shale averages about 9500 cal/g. Analyses show that the dried algal ooze from Mud Lake contains small quantities of higher fatty acids (C 12 –C 34 ), with C 16 being dominant. It also contains some unsaturated fatty acids and about 0.3 per cent of n-alkanes, predominantly odd carbons, with C 29 being dominant. A few qualitative analyses show that carotenoid pigments and terpenes are also present. A possible source of hydrocarbons is the long branched side chain of the chlorophyll molecule. Small crustaceans liberate this in the form of phytol, which, by dehydration, can go over into a series of saturated and unsaturated hydrocarbons, including phytanc and pristane, both of which are common in Green River oil shale. Search for other precursors of hydrocarbons continues.

GSA Bulletin↗

USGS1991

Upper Devonian biostratigraphy of Michigan Basin

The Late Devonian Michigan Basin was floored by the Middle and Upper Devonian Squaw Bay Limestone, which was deposited during the downwarping that produced the basin within a former Middle Devonian carbonate platform. The Squaw Bay comprises three beds, each having a different conodont fauna. The two upper beds, deposited during the transitans Zone, have different conodont biofacies that reflect this deepening. The basin was largely filled by the deep-water, anaerobic to dysaerobic, organic-rich, black Antrim Shale, which has a facies relationship with the prodeltaic, greenish gray Ellsworth Shale that prograded into the basin from the west. The Upper Devonian (Frasnian to Famennian) Antrim Shale is divided into four members, from base to top: the Norwood, Paxton, Lachine, and upper members. These members are more or less precisely dated by conodonts. The Norwood was deposited during the transitans Zone to Ancyrognathus triangularis Zone, and the Paxton was deposited from that zone probably through the linguiformis Zone at the end of the Frasnian. The overlying Lachine was deposited during the early Famennian and has yielded faunas of the Upper crepida and Lower rhomboidea Zones. Only the lower part of the upper member is exposed, and near Norwood, Michigan, it yielded conodonts of the Lower marginifera Zone. The widespread Famennian floating plant Protosalvinia (Foerstia) has not yet been found in outcrops of the Antrim, and should not be expected to occur except in the upper member or highest part of the Lachine Member. Its range in terms of conodont zones is from the Upper trachytera Zone through the Lower expansa Zone and possibly into the Middle expansa Zone. One known subsurface occurrence might be datable as rhomboidea or Lower marginifera Zone, depending on gamma ray correlations to outcrops. Black shale deposition ended when the Late Devonian mud delta of the Bedford Shale prograded across the Michigan Basin from the east and then retreated as the regressive Berea Sandstone was being deposited during the major eustatic sea-level fall that ended the Devonian. The Bedford was deposited during the Upper expansa to Lower praesulcata Zones, and the Berea was deposited during the Middle to Upper praesulcata Zones. Both formations contain the spore Retispora lepidophyta, which is a global indicator of latest Devonian age.

Michigan↗

USGS1999

Diving and foraging patterns of Marbled Murrelets (Brachyramphus marmoratus): Testing predictions from optimal-breathing models

The diving behavior of Marbled Murrelets (Brachyramphus marmoratus) was studied using telemetry along the Oregon coast during the 1995 and 1996 breeding seasons and examined in relation to predictions from optimal-breathing models. Duration of dives, pauses, dive bouts, time spent under water during dive bouts, and nondiving intervals between successive dive bouts were recorded. Most diving metrics differed between years but not with oceanographic conditions or shore type. There was no effect of water depth on mean dive time or percent time spent under water even though dive bouts occurred in depths from 3 to 36 m. There was a significant, positive relationship between mean dive time and mean pause time at the dive-bout scale each year. At the dive-cycle scale, there was a significant positive relationship between dive time and preceding pause time in each year and a significant positive relationship between dive time and ensuing pause time in 1996. Although it appears that aerobic diving was the norm, there appeared to be an increase in anaerobic diving in 1996. The diving performance of Marbled Murrelets in this study appeared to be affected by annual changes in environmental conditions and prey resources but did not consistently fit predictions from optimal-breathing models.

Canadian Journal of Zoology↗

USGS2022

Formation and distribution of coal balls in the Herrin Coal (Pennsylvanian), Franklin County, Illinois Basin, USA

Large areas of concentrated coal balls (permineralized peat) up to 4 m thick obstructed longwall mining in the Herrin Coal at the Old Ben No. 24 mine. The largest coal‐ball area mapped contained >1500 m 3 ; several areas contained >400 m 3 of coal balls. In‐mine mapping established that there were two types of roof (freshwater and marine), and that the coal balls were spatially correlated with the marine roof units. Regional studies and local data revealed that the younger, freshwater Energy Shale (mud) originally covered all of the peat deposited at the mine, but the mud was locally removed during a period of erosion. The great majority of coal balls are found within the coal seam, where they were permineralized in situ by carbonates. Some coal balls are found exposed on the eroded coal surface and others are within the channel fill associated with the erosion, which predates any marine sedimentation. Thus, the mineralization of the coal balls was synchronous with the erosion of the Energy Shale mud. Data from C‐and O‐isotope, geochemical, and mineralogical analyses of coal balls and associated materials were used to refine a depositional model of coal‐ball formation. The concentrated coal‐ball areas were created by the triggered degassing of CO 2 from partially decomposed peat in the presence of cations from fresh waters; 13 C/ 12 C ratios in these coal balls average −23.9‰. 13 C/ 12 C ratios as low as −34‰ occurring in the most concentrated coal balls are consistent with CO 2 produced by anaerobic oxidation of methane. Top‐of‐seam coal balls were formed later and show slight to strong marine influence; their 13 C/ 12 C ratios average −10.8‰.

Illinois↗

USGS2015

Rapid maturation of the muscle biochemistry that supports diving in Pacific walruses ( Odobenus rosmarus divergens )

Physiological constraints dictate animals’ ability to exploit habitats. For marine mammals, it is important to quantify physiological limits that influence diving and their ability to alter foraging behaviors. We characterized age-specific dive limits of walruses by measuring anaerobic (acid-buffering capacity) and aerobic (myoglobin content) capacities of the muscles that power hind (longissimus dorsi) and fore (supraspinatus) flipper propulsion. Mean buffering capacities were similar across muscles and age classes (a fetus, five neonatal calves, a 3 month old and 20 adults), ranging from 41.31 to 54.14 slykes and 42.00 to 46.93 slykes in the longissimus and supraspinatus, respectively. Mean myoglobin in the fetus and neonatal calves fell within a narrow range (longissimus: 0.92–1.68 g 100 g −1 wet muscle mass; supraspinatus: 0.88–1.64 g 100 g −1 wet muscle mass). By 3 months post-partum, myoglobin in the longissimus increased by 79%, but levels in the supraspinatus remained unaltered. From 3 months post-partum to adulthood, myoglobin increased by an additional 26% in the longissimus and increased by 126% in the supraspinatus; myoglobin remained greater in the longissimus compared with the supraspinatus. Walruses are unique among marine mammals because they are born with a mature muscle acid-buffering capacity and attain mature myoglobin content early in life. Despite rapid physiological development, small body size limits the diving capacity of immature walruses and extreme sexual dimorphism reduces the diving capacity of adult females compared with adult males. Thus, free-ranging immature walruses likely exhibit the shortest foraging dives while adult males are capable of the longest foraging dives.

Journal of Experimental Biology↗

USGS2010

Micromorphology and stable-isotope geochemistry of historical pedogenic siderite formed in PAH-contaminated alluvial clay soils, Tennessee, U.S.A

Alluvial clay soil samples from six boreholes advanced to depths of 400-450 cm (top of limestone bedrock) from the Chattanooga Coke Plant (CCP) site were examined micromorphologically and geochemically in order to determine if pedogenic siderite (FeCO3) was present and whether siderite occurrence was related to organic contaminant distribution. Samples from shallow depths were generally more heavily contaminated with polycyclic aromatic hydrocarbons (PAHs) than those at greater depth. The upper 1 m in most boreholes consisted of mixtures of anthropogenically remolded clay soil fill containing coal clinker, cinder grains, and limestone gravel; most layers of coarse fill were impregnated with creosote and coal tar. Most undisturbed soil (below 1 m depth) consisted of highly structured clays exhibiting fine subangular blocky ped structures, as well as redox-related features. Pedogenic siderite was abundant in the upper 2 m of most cores and in demonstrably historical (< 100 years old) soil matrices. Two morphologies were identified: (1) sphaerosiderite crystal spherulites ranging from 10 to 200 um in diameter, and (2) coccoid siderite comprising grape-like "clusters" of crystals 5-20 ??n in diameter. The siderite, formed in both macropores and within fine-grained clay matrices, indicates development of localized anaerobic, low-Eh conditions, possibly due to microbial degradation of organic contaminants. Stable-isotope compositions of the siderite have ??13C values spanning over 25%o (+7 to - 18%o VPDB) indicating fractionation of DIC by multiple microbial metabolic pathways, but with relatively constant ??18O values from (-4.8 ?? 0.66%o VPDB) defining a meteoric sphaerosiderite line (MSL). Calculated isotope equilibrium water ??18O values from pedogenic siderites at the CCP site are from 1 to 5 per mil lighter than the groundwater ??18O values that we estimate for the site. If confirmed by field studies in progress, this observation might call for a reevaluation of low-temperature siderite-water 18O fractionations. Investigations at the CCP site thus provide valuable information on the geochemical conditions under which siderite can form in modern soils, and thus insight on controls on siderite formation in ancient soils. Copyright ?? 2010, SEPM (Society for Sedimentary Geology).

Journal of Sedimentary Research↗