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Abundance and distribution of eelgrass (Zostera marina) and seaweeds at Izembek National Wildlife Refuge, Alaska, 2007–10

Eelgrass ( Zostera marina ) meadows are expansive along the lower Alaska Peninsula, supporting a rich diversity of marine life, yet little is known about their status and trends in the region. We tested techniques to inventory and monitor trends in the spatial extent and abundance of eelgrass in lagoons of the Izembek National Wildlife Refuge. We determined if Landsat imagery could be used to assess eelgrass spatial extent in shallow (less than 4 meter water depth) coastal waters of the refuge. We determined that this seagrass could be differentiated using Landsat imagery from other cover types (that is, channels and unvegetated tidal flats) with a high degree of accuracy (greater than 80 percent) in Izembek and Kinzarof Lagoons. Eelgrass meadows represented the largest cover type in Izembek (about 16,000 hectares) and Kinzarof (about 900 hectares) Lagoons, comprising between 45 and 50 percent of the spatial extent of these lagoons, respectively. When compared to estimates of spatial extent of eelgrass from previous studies, our results suggest little change in the spatial extent of eelgrass in Izembek Lagoon during the 28-year period 1978 through 2006. Preliminary mapping of eelgrass in other embayments indicated that this seagrass was also expansive in Big Lagoon (about 900 hectares; or 34 percent of the lagoon area) and Hook Bay (about 900 hectares; or 36 percent of the bay area) but not in Cold Bay (about 100 hectares; less than 5 percent of the bay area). We conducted an embayment-wide point sampling technique to assess aboveground biomass and distribution of eelgrass and seaweeds and presence of six macro-invertebrates during a 4-year period (2007–10). We determined that, when present, mean aboveground biomass of eelgrass was greater in Kinzarof Lagoon (182.5 plus or minus 12.1 grams dry weight per square meter) than in Izembek Lagoon (152.1 plus or minus 7.1 grams dry weight per square meter) in 2008–10, possibly reflecting the warmer sea temperatures and higher salinities found on the Gulf of Alaska side of the Alaska Peninsula. Seaweeds were more abundant in Kinzarof Lagoon than in Izembek Lagoon, surpassing aboveground biomass of eelgrass in both lagoons in 2008. Gastropods (4 percent of all points) and Caprella shrimp (25 percent) were the most common of the six macro-invertebrates surveyed in Izembek Lagoon, and Telmessus crab was the most common macro-invertebrate in Kinzarof Lagoon.

Alaska↗

Age, Growth and Reproduction of the Eastern Mudminnow (Umbra pygmaea) at the Great Swamp National Wildlife Refuge, New Jersey.

Umbra pygmaea DeKay (Eastern Mudminnow) is one of four species of Umbridae in North America. There is little published life-history information on the species within its native range, particularly on age, growth, and reproduction. This study focuses on these aspects of the life history of this fish at the Great Swamp National Wildlife Refuge in Morris County, NJ. A total of 336 fish of seven species was collected from June 1978 through May 1979, with the Eastern Mudminnow comprising 74% of the total. The average annual growth increment in total length for the Eastern Mudminnow was 15.3 2.06 mm, with age-1 fish averaging 40 mm total length and age-5 fish, the oldest collected, averaging 107 mm total length. The length-weight relationship was log10W = -5.291 + 3.182 log10TL mm for males and log10W = -4.999 + 3.032 log10TL mm for females. We observed no statistically significant sexually dimorphic differences in length-weight relationships in this population. The ratio of females to males increased from a low of 0.6 (predominance of male fish) at age-1 to a high of 4.6 (predominance of females) at age-5. Annual mortality for age 2–5 fish ranged from 40–76% with a mean of 59 13%. Age-specific fecundity estimates ranged from 250 eggs/female at age-1 to 2168 eggs/female at age-5. The relationship of number of mature ova to age was best described by the exponential function y = 149.29e0.5287x, where y = age-specific fecundity and x = age in years. Ova ranged from 0.1–0.2 mm in diameter in June and July and averaged 1.41 0.1 mm (range = 1.29–1.62 mm) in early February prior to spawning. Peak spawning occurred in mid-April at temperatures of 9–12 °C, and all females were spent by late April (13–15 °C).

Northeastern Naturalist↗

Estimating the effect of tidal marsh restoration on housing prices: A hedonic analysis in the Nisqually National Wildlife Refuge, Washington, USA

This study employs the hedonic pricing method and a rich spatial and temporal dataset from two counties in Washington, USA to determine the effect of the 2009 Nisqually Restoration project (NRP) on housing prices in adjacent communities. The NRP restored 308 hectares of wetlands via dike removal in the Billy Frank Jr. Nisqually National Wildlife Refuge (NNWR), leading to improvements in salmon and bird abundance and recreational opportunities. We find that the ecological improvements made by the NRP increased the value of homes within 0.5 mile of the refuge by $ 37,631; homes 0.5 to 1 mile by $ 10,489; and homes 1 to 1.5 miles by $ 31,186. Our findings are consistent with previous wetland hedonic price analyses and may be useful inputs in natural resource management and policy decision-making.

Washington↗

Hydrology Prior to Wetland and Prairie Restoration in and around the Glacial Ridge National Wildlife Refuge, Northwestern Minnesota, 2002-5

The Nature Conservancy (TNC) owned and managed 24,795 acres of mixed wetland, native prairie, farmland and woods east of Crookston, in northwestern Minnesota. The original wetlands and prairies that once occupied this land are being restored by TNC in cooperation with many partners and are becoming part of the Glacial Ridge National Wildlife Refuge. Results of this study indicate that these restorations are likely to have a substantial effect on the local hydrology. Water occurs within the study area on the land surface, in surficial aquifers, and in buried aquifers of various depths, the tops of which are 50 to several hundred feet below the land surface. Surficial aquifers are generally thin (about 20 feet), narrow (several hundred feet), and long (tens of miles). Estimates of the horizontal hydraulic conductivity of surficial aquifers were 2.7?300 feet per day. Buried aquifers underlie much of the study area, but interact with surficial aquifers only in isolated areas. In these areas, water flows directly from buried to surficial aquifers and forms a single aquifer as much as 78 feet thick. The surface?water channel network is modified by several manmade ditches that were installed to remove excess water seasonally and to drain wetlands. The channels of the network lie primarily parallel to the beach ridges but cut through them in places. Back?beach basin wetlands delay and reduce direct runoff to ditches. Recharge to the surficial aquifers (10.97?25.08 inches per year during 2003?5) is from vertical infiltration of rainfall and snowmelt (areal recharge); from surface waters (particularly ephemeral wetlands); and from upward leakage of water from buried aquifers through till confining units (estimated at about 1 inch per year). Areal recharge is highly variable in space and time. Water leaves (discharges from) the surficial aquifers as flow to surface waters (closed basins and ditches), evapotranspiration, and withdrawals from wells. Unmeasured losses (primarily discharge to ungaged (closed) basins) were 53?115 percent of areal recharge during 2003?5, while discharge to ditches that leave the study area was 17?41 percent. Discharge over 100 percent of areal recharge indicates a loss in ground?water storage. During the dry year of 2003, substantial ground water (about one?third of annual areal recharge) was released from aquifer storage but was replenished quickly during the subsequent normal year. Shallow ground?water flow is complex, with water in surficial aquifers, ditches, and wetlands part of a single hydrologic system. The ages determined for surficial ground?water samples were less than 15 years old, and one?third (8 of 24) were less than 5 years old, substantiating the close connection of surficial ground water to the land surface. During the study, 68?81 percent of water left the area through unmeasured surface?water losses (primarily evapotranspiration), which is 2? to 4?times that leaving through the ditch system. Base flow in ditches (ground?water discharge) was 30 to 71 percent of all ditch flow. Mean annual runoff in all gaged basins except SW3 (2.26 inches per year) was similar (3.69?4.12 inches per year). The quality of water samples from surficial aquifers and surface water collected in the study area was generally suitable for most uses but was variable. Most ground? and surface?water samples were dominated by calcium, magnesium, and bicarbonate ions. About one?quarter of surficial ground?water samples contained nitrate at concentrations greater than the U.S. Environmental Protection Agency?s (USEPA) Maximum Contaminant Level for human consumption. The median concentration of dissolved phosphorus ranged from 0.0108 milligrams per liter as phosphorus (mg/L?P) to 0.0293 mg/L?P. Nutrient concentrations in ditches were generally above the USEPA nutrient guidelines for reference streams in the area. Water samples contained detectable concentrations of atrazine, acetachlor, metolachlor, pendimethalin

Scientific Investigations Report↗

Atlantic white cedar plantings in St. Tammany Parish, Louisiana, and the Bogue Chitto National Wildlife Refuge, Mississippi

Populations of Atlantic white cedar (Chamaecyparis thyoides (L.) B.S.P.) growing at the extreme western range of the species are in danger of being lost, and information on the ecology of these populations is limited. Seeds and seedlings ("wildlings") were collected near Vancleave, MS. The wildlings were transplanted to bay-head sites on Bogue Chitto National Wildlife Refuge where canopy gaps had been created in early 1989. Seeds were germinated, grown in a greenhouse, and transplanted a year later to three locations within a slash pine plantation: a bedded and fertilized site, the margin of a cypress pond, and a wet gap, in St. Tammany Parish, Lousiana. These plantings were established to study survival and growth in different habitat types, to compare seedlings grown from seed versus transplanted wildlings, and to attempt to generate some interest in planting Atlantic white-cedar in the extreme western portion of its natural range. Overall seedling survival averaged 94 percent after five growing seasons, although significant differences were detected among sites. Mean height per site in 1994 was significantly greater at the bedded and fertilized site (233 centimeters), and lowest at the slash pine site (91 centimeters). Growth at the cypress pond site was intermediate (158 centimeters). Wildlings at the bay-head sites had lower survival rates (75 and 64 percent), but average heights for the two sites were similar to those of the seedlings (265 and 165 centimeters). These results show that plantings involving either local seed or wildlings are viable choices for regenerating Atlantic white-cedar in southern Mississippi and eastern Louisiana.

Louisiana; Mississippi↗

Pathology of mouse (Mus musculus) predation on Laysan albatross (Phoebastria immutabilis) on Midway Atoll National Wildlife Refuge

Invasive rodents on islands have adverse effects on native birds in island ecosystems, and rats are the most common culprits. Recently, house mice ( Mus musculus ) in the South Atlantic were found preying on three species of albatross chicks. Here, we show that house mice can also prey on nesting adult Laysan Albatross ( Phoebastria immutabilis ) on Midway Atoll National Wildlife Refuge (US). In contrast to mouse attacks on albatross in the South Atlantic, where mice targeted the rump and crown of chicks, on Midway, mice targeted nesting adults mainly on the back. For both regions, the outcome was similar with reduced nesting success. In the case of Midway, reduced nesting success was due to nest abandonment or mortality of one or both parents because of secondary bacterial infections. Mouse-induced mortality of adult albatross has the potential to have a more potent demographic effect because of their much higher natural survivorship once they reach adulthood.

Midway Atoll↗

Distribution of eelgrass (Zostera marina) in coastal waters adjacent to Togiak National Wildlife Refuge, Alaska

Declines in the distribution and abundance of seagrasses worldwide have prompted a need for baseline distribution maps of eelgrass ( Zostera marina ) in Alaska. We used high-resolution digital-color aerial photography and multi-spectral satellite imagery to map the distribution and spatial extent of eelgrass at 21 sites in coastal waters adjacent to Togiak National Wildlife Refuge (TNWR) in northwestern Bristol Bay and southern Kuskokwim Bay. The total spatial extent of eelgrass meadows was estimated to be 6,489 hectare (ha) almost equally divided between Bristol Bay (3,001 ha) and Kuskokwim Bay (3,488 ha). The four largest eelgrass beds occurred in Chagvan Bay (1,933 ha), the north side of Hagemeister Island (1,168 ha), Goodnews Bay (874 ha), and Nanvak Bay (599 ha). This report provides key baseline data useful for establishing a monitoring plan to assess trends in eelgrass along the coast of TNWR.

Alaska↗

Five hydrologic and landscape databases for selected National Wildlife Refuges in the Southeastern United States

This report serves as metadata and a user guide for five out of six hydrologic and landscape databases developed by the U.S. Geological Survey, in cooperation with the U.S. Fish and Wildlife Service, to describe data-collection, data-reduction, and data-analysis methods used to construct the databases and provides statistical and graphical descriptions of the databases. Six hydrologic and landscape databases were developed: (1) the Cache River and White River National Wildlife Refuges (NWRs) and contributing watersheds in Arkansas, Missouri, and Oklahoma, (2) the Cahaba River NWR and contributing watersheds in Alabama, (3) the Caloosahatchee and J.N. “Ding” Darling NWRs and contributing watersheds in Florida, (4) the Clarks River NWR and contributing watersheds in Kentucky, Tennessee, and Mississippi, (5) the Lower Suwannee NWR and contributing watersheds in Georgia and Florida, and (6) the Okefenokee NWR and contributing watersheds in Georgia and Florida. Each database is composed of a set of ASCII files, Microsoft Access files, and Microsoft Excel files. The databases were developed as an assessment and evaluation tool for use in examining NWR-specific hydrologic patterns and trends as related to water availability and water quality for NWR ecosystems, habitats, and target species. The databases include hydrologic time-series data, summary statistics on landscape and hydrologic time-series data, and hydroecological metrics that can be used to assess NWR hydrologic conditions and the availability of aquatic and riparian habitat. Landscape data that describe the NWR physiographic setting and the locations of hydrologic data-collection stations were compiled and mapped. Categories of landscape data include land cover, soil hydrologic characteristics, physiographic features, geographic and hydrographic boundaries, hydrographic features, and regional runoff estimates. The geographic extent of each database covers an area within which human activities, climatic variation, and hydrologic processes can potentially affect the hydrologic regime of the NWRs and adjacent areas. The hydrologic and landscape database for the Cache and White River NWRs and contributing watersheds in Arkansas, Missouri, and Oklahoma has been described and documented in detail (Buell and others, 2012). This report serves as a companion to the Buell and others (2012) report to describe and document the five subsequent hydrologic and landscape databases that were developed: Chapter A—the Cahaba River NWR and contributing watersheds in Alabama, Chapter B—the Caloosahatchee and J.N. “Ding” Darling NWRs and contributing watersheds in Florida, Chapter C—the Clarks River NWR and contributing watersheds in Kentucky, Tennessee, and Mississippi, Chapter D—the Lower Suwannee NWR and contributing watersheds in Georgia and Florida, and Chapter E—the Okefenokee NWR and contributing watersheds in Georgia and Florida.

Alabama, Arkansas, Florida, Georgia, Kentucky, Mis↗

Sedimentation History of Halfway Creek Marsh, Upper Mississippi River National Wildlife and Fish Refuge, Wisconsin, 1846-2006

The history of overbank sedimentation in the vicinity of Halfway Creek Marsh near La Crosse, Wis., was examined during 2005?06 by the U.S. Geological Survey and University of Wisconsin?Madison as part of a broader study of sediment and nutrient loadings to the Upper Mississippi River bottomlands by the U.S. Environmental Protection Agency, U.S. Fish and Wildlife Service, and U.S. Geological Survey. Historical sedimentation patterns and rates were interpreted from field-scale topographic surveys and sediment cores collected from the marsh and upstream flood plains. Historical maps and aerial photographs were used to establish the timing of disturbances and to document changes in channel patterns after Euro-American settlement (post 1846). Episodic overbank sedimentation patterns and rates were linked to watershed agricultural activity, large floods, artificial levee construction, channel alterations, and dam failures over the past 160 years. These forces affected sedimentation on and between levees, the development of alluvial fans and flood-plain splays, and the general pattern of flood-plain sedimentation through the upper and lower marsh. Historical overbank deposits, episodically deposited after about 1860, are as much as 6 feet thick in the upper marsh and as much as 4 feet thick in the lower marsh, representing a total volume of approximately 1.8 million cubic yards. These stratified deposits consist of multiple layers of silt and clay, very fine to fine sand, and some medium to very coarse sand. Coarse-grained deposits are associated with flood-plain splays caused by breaches in artificial levees during large floods. Estimated sedimentation rates were highest from 1919 to 1936 [26,890 cubic yards per year (yd3/yr)] and exceeded by about 30 times the 1846?85 rate of 920 yd3/yr and exceeded by 7 times the 1994?2006 rate of 3,740 yd3/yr. The 1994?2006 sedimentation rate was the lowest since Euro-American settlement, but natural levees along the 1994?2006 channel of Halfway Creek through the lower marsh continued to form and are currently (2006) about 1 foot higher than the surrounding marsh. Natural levee building in the lower marsh from 1994?2006 was accentuated by the lack of overbank sediment storage in the upper marsh. The historical storage of sediment in the upper and lower marsh affects modern streamflow and sediment transport processes of Halfway Creek and Sand Lake Coulee through the marsh, and it also affects marsh vegetation and wildlife habitat. Results from this investigation will help improve the understanding of how past overbank sedimentation patterns continue to influence modern and future water quality, sediment transport, nutrient loads, and water-related resources in riparian habitats common to the Upper Mississippi River National Wildlife and Fish Refuge.

Scientific Investigations Report↗

Modeling potential impacts of the Garrison Diversion Unit project on Sand Lake and Arrowwood National Wildlife Refuges: a feasibility analysis

The Garrison Diversion Unit (GDU) of the Pick-Sloan Missouri Basin program was authorized in 1965, with the purpose of diverting Missouri River water to the James River for irrigation, municipal and industrial water supply, fish and wildlife habitat, recreation, and flood control. The project was reauthorized in 1986, with the specification that comprehensive studies be conducted to address a variety of issues. One of these ongoing studies addresses potential impacts of GDU construction and operation on lands of the National Wildlife Refuge (NWR) system, including Arrowwood and Sand Lake Refuges (the Refuges) on the James River. A number of concerns at these Refuges have been identified; the primary concerns addressed in this report include increased winter return flows, which would limit control of rough fish; increased turbidity during project construction, which would decrease production of sago pondweed; and increased water level fluctuations in the late spring and early summer, which would destroy the nests of some over-water nesting birds. The facilitated workshop described in this report was conducted February 18-20, 1987, under the joint sponsorship of the U.S. Bureau of Reclamation, the U.S. Fish and Wildlife Service, and the North Dakota Game and Fish Department. The primary objectives of the workshop were to evaluate the feasibility of using simulation modeling techniques to estimate GDU impacts on Arrowwood and Sand Lake Refuges and to suggest enhancements to the James River Refuge monitoring program. The workshop was structured around the formulation of four submodels: a Hydrology and Water Quality submodel to simulate changes in Refuge pool elevations, turnover rates, and water quality parameters (e.g., total dissolved solids, turbidity, dissolved oxygen, nutrients, water temperature, pesticides) due to GDU construction and operation; a Vegetation submodel to simulate concomitant changes in wetland communities (e.g., sago pondweed, wet meadows, deep and shallow marsh); a Fish submodel to estimate changes in abundance or biomass of rough fish (carp, buffalo) and sportfish (northern pike); and a Wildlife submodel to calculate indices of waterfowl abundance or habitat suitability (e.g., for mallards, western grebes, migrating diving ducks, white-faced ibis, egrets, over-water nesters). Submodels considered weekly to monthly changes in pools within a Refuge over a time horizon of 30-50 years. Based on workshop discussions and past experience with impact analysis modeling, a phased modeling approach was recommended for the James River Refuges analysis. The first phase would involve two modeling efforts. The existing Sand Lake hydrology model, and a similar one developed for Arrowwood NWR, would be validated and used to predict changes on pool elevations and winter inflows to each pool for a variety of GDU alternatives. Outputs from simulations would then be evaluated in terms of potential fish and wildlife impacts. For example, the models could generate indices comparing the magnitude and timing of winter inflows for pre- and postproject conditions; fisheries biologists could then use these indices to better quantify their concerns relative to potential changes in the frequency of rough-fish control. The other modeling effort in the first phase would involve developing a sago pondweed growth model to integrate Refuge monitoring data and existing literature and perhaps to address some questions concerning turbidity impacts. A second phase of simulation modeling would be undertaken only if the initial analyses of hydrologic outputs indicated significant potential problems and if monitoring and research projects had clarified some of the biological and physical processes that cannot be modeled reliably at the present time (e.g., resuspension of sediments by carp, immigration and winter mortality of fish, loss of waterfowl nests due to wave action). The second phase would attempt to develop an integrated impact assessment model. In order to address some of the biological and physical processes that presently are not well understood, a number of studies and enhancements to the Refuge monitoring program were suggested. The Hydrology and Water Quality workgroup recommended increasing turbidity and dissolved oxygen sampling, dropping expensive analysis of some trace elements, adding more pesticide analysis (including some biological monitoring), and developing better area-capacity data for the Sand Lake hydrology model. The Vegetation workgroup suggested expanding the number of monitoring stations, monitoring photosynthetically active radiation by depth, and modifying the biomass sampling procedure and schedule. Also suggested were additional analyses of existing Refuge monitoring data and additional field studies concerning sago growth under a variety of environmental conditions and effects of rough fish density on sago. A careful examination of Refuge narrative reports was recommended by the Fish workgroup to characterize conditions that led to various rates of winter-kill. Monitoring enhancement related to a better understanding of fish population dynamics included increasing dissolved oxygen monitoring, continuing present monitoring of fish movement upstream from Jamestown Reservoir into Arrowwood NWR, initiating similar efforts for upstream movement into Sand Lake NWR and downstream movements into both Refuges, and augmenting the present gillnetting program (or replacing it) with sampling for population and age/size structure estimates. The Wildlife workgroup suggested estimating the relative density of mallard nests in over-water and wet meadow nesting areas, estimating the number of western grebe nests lost due to wave action, delineating wet meadows on the Refuge vegetation maps, estimating annual tuber consumption by birds, and monitoring insect/macroinvertebrate abundance. The workgroup also suggested research studies to better understand the relationships between food supplies and the growth and survival of ducklings and young grebes. the workshop discussions also helped identify some suggestions for modifying project features that, if feasible from an engineering and operational standpoint, would reduce impacts on Refuge lands. These suggestions included: designing drains with control structures or small "reregulation" reservoirs to hold winter return flows that might adversely affect rough fish control, spreading construction activities over a number of years to reduce potential impacts of turbidity on sago pondweed in any single year, scheduling construction to occur after the spring sprouting and elongation growth stages to reduce impacts on sago pondweed, and installing "quick acting" control structures at Arrowwood NWR to reduce pool level fluctuations that might destroy nests of some over-water nesting waterfowl.

Report↗

Breeding shorebird surveys in the Arctic National Wildlife Refuge, Alaska, suggest population declines over two decades for most species

Shorebird populations are declining globally but it generally remains unclear how those declines translate to changes at the regional scale. We conducted the first longitudinal surveys of breeding shorebirds in Alaska under the Program for Regional and International Shorebird Monitoring (PRISM), resurveying the Coastal Plain (1002 Area) of the Arctic National Wildlife Refuge (NWR) in 2019 and 2022 to compare with initial surveys conducted in 2002 and 2004. Our goals were to (1) estimate contemporary population sizes of breeding shorebirds across this 6,249 km 2 area, and (2) assess population trends for the species detected in both survey periods. We estimated population sizes for 16 species, with a combined total of 135,178 (95% CI: 113,532–156,824) in 2019 and 2022—a decline of approximately 17% (90% CI: –34% to + 3%) from 2002 and 2004 when the same survey methods were used. Four species showed a statistically significant decrease (α = 0.10): Calidris alpina arcticola (Dunlin), Limnodromus scolopaceus (Long-billed Dowitcher), Phalaropus lobatus (Red-necked Phalarope), and P. fulicarius (Red Phalarope). Only C. melanotos (Pectoral Sandpiper) showed a significant increase. Overall, 5 of 10 species—and all species combined—had a > 90% probability of decline. Population changes for the polygamous species (i.e., Phalaropus sp. and C. melanotos ), which show irruptive breeding and low breeding site fidelity, may reflect temporary immigration or emigration driven by annual environmental variation, rather than true population change. Nevertheless, the overall pattern of declines aligns with migration surveys outside the Arctic. These findings highlight the vulnerability of Arctic-breeding shorebirds to threats throughout their annual cycles and underscore the potential for sustained long-term monitoring in this rapidly changing region to inform effective, flyway-scale conservation strategies across the Western Hemisphere.

Alaska↗

Precipitation, density, and population dynamics of desert bighorn sheep on San Andres National Wildlife Refuge, New Mexico

Understanding the determinants of population size and performance for desert bighorn sheep (Ovis canadensis mexicana) is critical to develop effective recovery and management strategies. In arid environments, plant communities and consequently herbivore populations are strongly dependent upon precipitation, which is highly variable seasonally and annually. We conducted a retrospective exploratory analysis of desert bighorn sheep population dynamics on San Andres National Wildlife Refuge (SANWR), New Mexico, 1941-1976, by modeling sheep population size as a function of previous population sizes and precipitation. Population size and trend of desert bighorn were best and well described (R 2=0.89) by a model that included only total annual precipitation as a covariate. Models incorporating density-dependence, delayed density-dependence, and combinations of density and precipitation were less informative than the model containing precipitation alone (??AlCc=8.5-22.5). Lamb:female ratios were positively related to precipitation (current year: F1,34=7.09, P=0.012; previous year: F1,33=3.37, P=0.075) but were unrelated to population size (current year. F1,34=0.04, P=0.843; previous year: F1,33 =0.14, P=0.715). Instantaneous population rate of increase (r) was related to population size (F1,33=5.55; P=0.025). Precipitation limited populations of desert bighorn sheep on SANWR primarily in a density-independent manner by affecting production or survival of lambs, likely through influences on forage quantity and quality. Habitat evaluations and recovery plans for desert bighorn sheep need to consider fundamental influences on desert bighorn populations such as precipitation and food, rather than focus solely on proximate issues such as security cover, predation, and disease. Moreover, the concept of carrying capacity for desert bighorn sheep may need re-evaluation in respect to highly variable (CV =35.6%) localized precipitation patterns. On SANWR carrying capacity for desert bighorn sheep was zero when total annual precipitation was <28.2 cm.

Wildlife Society Bulletin↗

Summary of oceanographic and water-quality measurements in Rachel Carson National Wildlife Refuge, Wells, Maine, in 2013

Suspended-sediment transport is a critical element controlling the geomorphology of tidal wetland complexes. Wetlands rely on organic material and inorganic sediment deposition to maintain their elevation relative to sea level. The U.S. Geological Survey performed observational deployments to measure suspended-sediment concentration and water flow rates in the tidal channels of the wetlands in the Rachel Carson National Wildlife Refuge in Wells, Maine. The objective was to characterize the sediment-transport mechanisms that contribute to the net sediment budget of the wetland complex. We deployed a meteorological tower, optical turbidity sensors, and acoustic velocity meters at sites on Stephens Brook and the Ogunquit River between March 27 and December 9, 2013. This report presents the time-series oceanographic and atmospheric data collected during those field studies. The oceanographic parameters include water velocity, depth, turbidity, salinity, temperature, and pH. The atmospheric parameters include wind direction, speed, and gust; air temperature; air pressure; relative humidity; short wave radiation; and photosynthetically active radiation.

Maine↗

Effects of persistent energy-related brine contamination on amphibian abundance in national wildlife refuge wetlands

To inform sustainable energy development, it is important to understand the ecological effects of historical and current production practices and the persistence of those effects. The Williston Basin is one of North America's largest oil production areas and overlaps the Prairie Pothole Region, an area densely populated with wetlands that provide important wildlife habitat. Although historical disposal practices that released chloride-rich waters (brines) produced during oil extraction into the environment are no longer used, brine spills still occur frequently. We sampled 33 wetlands for three amphibian species in Montana and North Dakota during 2015–2017, primarily on National Wildlife Refuges, and used N-mixture models to determine how abundance varied with evidence of brine contamination. To provide insight into effects of historical versus contemporary contamination, we also estimated the association of well density and age with water quality and amphibian abundance. Abundance of boreal chorus frog ( Pseudacris maculata ) larvae declined most rapidly in response to increased chloride (range: 0.04–17,500 mg/L), followed by the northern leopard frog ( Lithobates [ Rana ] pipiens ) and barred tiger salamander ( Ambystoma mavortium ). Water quality and population- and community-level abundance of amphibians were more strongly related to nearby wells (≤800 m) installed before 1982 than to wells installed since 1982. These results suggest historical brine management practices were the primary driver of contamination and reduced amphibian abundance in wetlands we sampled, reflecting multi-decadal ecological effects. These persistent effects also underscore the critical need for tools to restore landscapes affected by brine contamination.

Montana, North Dakota, South Dakota↗

Quantifying post-colonial peat carbon loss from a drained forested peatland, Great Dismal Swamp National Wildlife Refuge, USA

Peatland carbon storage is increasingly threatened by the combination of land-use change and climate variability, though carbon losses from land-use changes that span centuries are difficult to quantify, particularly in systems where little undisturbed area remains. Here we use a combination of vegetation change, fire history, and calculations of excess ash mass to quantify carbon loss in the Great Dismal Swamp National Wildlife Refuge (GDS NWR), USA, a highly impacted oligotrophic temperate peat swamp. Our results indicate that ditch construction that began in the Colonial Era in the late 1700s and continued into the mid-20th century across the swamp resulted in shifts from cypress-tupelo swamps to a combination of maple-gum and pine pocosin forests, consistent with drying surface conditions. Two large smoldering fires (2008, 2011) that were exacerbated by surface drainage, shifted vegetation from swamp to marsh, consumed peat over 25 km 2 , and caused losses of 1.05–1.34 Tg C due to peat burning. Across the Refuge as a whole, up to 48.2 Tg C has been lost to peat oxidation since ditch construction. Both stocks and rates of carbon loss remain higher than post-disturbance accumulation across most of GDS NWR, suggesting that existing efforts to block drainages to elevate water tables may not be enough to offset carbon losses. Rewetting heavily impacted surface peats may reduce peat oxidation and carbon loss, and shift vegetation toward hydrologic conditions preferred by pre-disturbance cypress-tupelo swamps.

North Carolina, Virginia↗

Trends and causes of historical wetland loss, Sabine National Wildlife Refuge, southwest Louisiana

Prior U.S. Geological Survey studies (Open-File Reports 2005-1216 and 2009-1158) examined historical land- and water-area changes and estimated magnitudes of land subsidence and erosion at 10 wetland sites in the Mississippi River delta plain. The present study extends that work by analyzing interior wetland loss and relative magnitudes of subsidence and erosion at five additional wetland sites in Sabine National Wildlife Refuge (SNWR) in the western chenier plain. The study sites were selected because their geologic setting differed from that of the delta plain; also, although the refuge marshes had been managed partly to minimize wetland loss, interior wetland losses there were extensive. Historical aerial photography, datum-corrected marsh elevations and water depths, and sediment cores were integrated to evaluate historical land- and water-area changes at SNWR. The thickness of the uppermost Holocene sediments (peat and organic-rich mud) and the elevation of stratigraphic contacts were compared at marsh and open-water sites across areas of formerly continuous marsh to estimate magnitudes of recent elevation loss caused by vertical erosion and subsidence. Results of these analyses indicate that erosion greatly exceeded subsidence at most of the core sites, although both processes have contributed to historical wetland loss. Comparison of these results with results of our prior studies indicates that magnitudes of subsidence and total accommodation space that formed in the western chenier plain were less than those in the delta plain. Compared with the delta plain, where subsidence generally exceeded erosion and peat thicknesses were so great that peat was preserved even where erosion was greater than subsidence, the SNWR peats are thin and were absent (eroded) at most open-water sites. Although historical subsidence rates in the chenier plain are substantially lower than most of the same rates in the delta plain, the temporal and spatial trends of rapid wetland loss, highest rates of land-surface subsidence, and high rates of oil-and-gas production are similar, indicating that historical wetland loss was likely initiated by similar processes (deep-subsurface subsidence) in both regions.

Open-File Report↗

Differential changes in the onset of spring across US National Wildlife Refuges and North American migratory bird flyways

Warming temperatures associated with climate change can have indirect effects on migratory birds that rely on seasonally available food resources and habitats that vary across spatial and temporal scales. We used two heat-based indices of spring onset, the First Leaf Index (FLI) and the First Bloom Index (FBI), as proxies of habitat change for the period 1901 to 2012 at three spatial scales: the US National Wildlife Refuge System; the four major bird migratory flyways in North America; and the seasonal ranges (i.e., breeding and non-breeding grounds) of two migratory bird species, Blue-winged Warbler ( Vermivora cyanoptera ) and Whooping Crane ( Grus americana ). Our results show that relative to the historical range of variability, the onset of spring is now earlier in 76% of all wildlife refuges and extremely early (i.e., exceeding 95% of historical conditions) in 49% of refuges. In all flyways but the Pacific, the rate of spring advance is generally greater at higher latitudes than at lower latitudes. This differential rate of advance in spring onset is most pronounced in the Atlantic flyway, presumably because of a “warming hole” in the southeastern US. Both FLI and FBI have advanced markedly in the breeding ranges–but not the non-breeding ranges–of the two selected bird species, albeit with considerable intra-range variation. Differences among species in terms of migratory patterns and the location and extent of seasonal habitats, as well as shifts in habitat conditions over time, may complicate predictions of the vulnerability of migratory birds to climate change effects. This study provides insight into how differential shifts in the phenology of disparate but linked habitats could inform local- to landscape-scale management strategies for the conservation of migratory bird populations.

PLoS ONE↗

Changes in the prevalence of avian disease and mosquito vectors at Hakalau Forest National Wildlife Refuge: a 14-year perspective and assessment of future risk

Throughout the main Hawaiian Islands, introduced mosquito-borne disease has had, and continues to have, a profound impact on the distributions and abundance of native Hawaiian forest birds. Populations of remaining native forest birds are largely restricted to high elevation forests where mean temperatures are marginal for vector and parasite development and limited availability of larval mosquito habitat constrains mosquito populations and disease transmission. Hakalau Forest National Wildlife Refuge (HFNWR) was established for the preservation of endemic avifauna in 1985. Since its creation, native bird communities there have remained intact and most species populations are stable or increasing. However, avian malaria had been detected at HFNWR in the past and, in light of documented climate change, new concerns have been raised regarding the long-term fate of the refuge&rsquo;s forest birds. To examine the possible changes in avian malaria transmission at HFNWR we sampled forest birds for blood parasites, trapped adult mosquitoes and surveyed larval mosquito habitat at three sites during 2012 and compared our results with similar data collected between 1998 and 1999. We tested blood samples by polymerase chain reaction (PCR), immunoblotting, and microscopy to determine prevalence of acute and chronic infection and used attractive gravid traps to sample the vector mosquito Culex quinquefasciatus. Our study documented spatial trends and temporal changes in the prevalence of avian malaria, mosquito presence, larval mosquito habitat and feral pig activity at HFNWR. We found evidence of local transmission in high elevation forests, a general pattern of increasing prevalence at lower elevations and along a South to North gradient and a two-fold decrease in the prevalence of avian malaria in the intervening 14 years. Despite considerable effort, we were unable to detect larval C. quinquefasciatus and captured only one adult indicating that the vector of avian malaria has a very limited presence at HFNWR. We did, however, document the establishment of another invasive mosquito, Aedes japonicus japonicus , and its occurrence in tree fern cavities and rock pools as larval habitat in the lower forests of HFNWR. We suggest that interspecific competition by A. j. japonicus and predation by a suite of native predators may provide biotic resistance to the establishment of permanent C. quinquefasciatus populations. While current predictions of climate change in the Hawaiian Islands include a gradual warming and enhanced transmission by mid-century, the current cooling trend recorded at high elevation HFNWR illustrates the importance of monitoring to document fine scale temporal and site specific changes in prevalence. Long term changes in precipitation may have a more profound effect on local transmission of malaria than temperature and we may have already seen some potential impacts of an extended drought at HFNWR with a decrease in feral pig activity and pig associated larval mosquito habitat and increases in stream-associated larval mosquito habitat.

Hawaii↗