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Effects of experimental overgrowth on survival and change in the turf assemblage of a giant kelp forest

Crustose coralline algae were the prevalent cover among sessile organisms that paved or grew near the substratum, and also the most commonly overgrown species in a giant kelp Macrocystis pyrifera (L.) C.A. Agardh forest located off San Nicolas Island, California. Giant kelp was the largest and most conspicuous species that overgrew large patches of the substrata; overgrowth among turf organisms also appeared common. To determine the effects of giant kelp holdfasts on crustose coralline algae and other turf organisms, “artificial holdfasts” were placed on 0.125-m 2 plots for 5, 8 and 12 months. In these treatments, 50–57% of the crustose coralline algae survived. Because these algae also recruited while covered, the total cover (survivorship plus recruitment) differed by only 7–26% from that sampled at the start of the study. The decline of these algae in control plots was similar to that in the treatment plots mostly because of overgrowth by sessile invertebrates. Bryozoans increased markedly on the control plots, whereas 0–12% survived in the treatment plots. Bryozoans and sponges also recruited under the artificial holdfasts. Some arborescent turf algae survived in the 5- and 8-month treatments; articulated coralline algae survived better than did foliose algae. High survival recruitment of crustose coralline algae while overgrown contributed to their prevalence in benthic communities.

Journal of Experimental Marine Biology and Ecology

Diflubenzuron Hazards to Fish, Wildlife and Invertebrates: A Synoptic Review

Diflubenzuron (1(4chlorophenyl)3(2,6difluorobenzoyl)urea), also known as dimilin, is a potent broad spectrum insect growth regulator that interferes with chitin synthesis at time of molting and is effective in controlling immature stages of insects. Diflubenzuron was approved for domestic use in 1976 to control gypsy moth (Lymantria dispar), and in 1979 against the cotton boll weevil (Anthonomus grandis). By 1989 this compound was also registered for domestic use against mosquitos, forest Lepidoptera, mushroom flies, and leaf-eating insect pests of citrus, woody ornamentals, vegetables, and fruit. Diflubenzuron seldom persists for more than a few days in soil and water. When used properly in forest management, it is unlikely to be leached into ground water from the application site. Degradation in water and soil is most rapid when small particle formulations are applied; microorganisms are abundant; and at elevated pH, temperature, and organic loading. Chemical and biological processes initially yield 2,6difluorobenzoic acid and 4chlorophenylurea.

Contaminant Hazard Reviews

A comparison of selected diversity, similarity, and biotic indices for detecting changes in benthic-invertebrate community structure and stream quality

Implementation of advanced wastewater treatment at the two municipal wastewater-treatment plants for Indianapolis, Indiana, resulted in substantial improvement in the quality of the receiving stream and significant changes in the benthic-invertebrate community. Diversity, similarity, and biotic indices were compared to determine which indices best reflected changes in the composition of the biota in the river. None of the indices perfectly reflected the changes in river quality or community structure. Similarity indices, especially percentage similarity, exhibit the most promise of the three classes of indices. Diversity indices were least useful, wrongly indicating that water quality deteriorated after the upgrade of the wastewater-treatment plants. The most descriptive tool in analyzing the data was the percentage of Ephemeroptera, Plecoptera, and Trichoptera (EPT) taxa present. Using a mixture of indices and other analytical tools, such as EPT, in the analysis of biological data will ensure the most effective investigations of water quality.

Archives of Environmental Contamination and Toxico

Effect of a levee setback on aquatic resources using two-dimensional flow and bioenergetics models

Watershed restoration is the focus of many resource managers and can include a multitude of restoration actions each with specific restoration objectives. For the White River flowing through the cities of Pacific and Sumner, Washington, a levee setback has been proposed to reconnect the river with its historical floodplain to help reduce flood risks, as well as provide increased habitat for federally listed species of salmonids. The study presented here documents the use of a modeling framework that integrates two-dimensional hydraulic modeling with process-based bioenergetics modeling for predicting how changes in flow from reconnecting the river with its floodplain affects invertebrate drift density and the net rate of energy intake of juvenile salmonids. Modeling results were calculated for flows of 25.9 and 49.3 cubic meters per second during the spring, summer, and fall. Predicted hypothetical future mean velocities and depths were significantly lower and more variable when compared to current conditions. The abundance of low energetic cost and positive growth locations for salmonids were predicted to increase significantly in the study reach following floodplain reconnection, particularly during the summer. This modeling framework presents a viable approach for evaluating the potential fisheries benefits of reconnecting a river to its historical floodplain that integrates our understanding of hydraulic, geomorphology, and organismal biology.

Washington

Patterns of use and distribution of king eiders and black scoters during the annual cycle in northeastern Bristol Bay, Alaska

Northeastern Bristol Bay, Alaska, which includes three large estuaries, is used by multiple sea duck species during the annual cycle. Limited aerial surveys indicate that this area supports tens of thousands of king eiders and black scoters during spring migration and the autumn molt. Existing satellite telemetry data were used to assess the temporal patterns of habitat use and spatial distribution of king eiders and black scoters in northeastern Bristol Bay throughout the annual cycle. King eiders used northeastern Bristol Bay during all months of the annual cycle and black scoters used the area during spring through fall. Both species exhibited a similar seasonal pattern of use that corresponded with the timing of life-cycle stages. Abundance of both species was highest during spring migration and the autumn molting period and lowest during summer. Use by king eiders did not occur during all winter months in every year of the study. King eiders were more broadly distributed than black scoters and were located farther from shore in deeper water. Core use areas had minimal overlap, suggesting a degree of spatial segregation between species and a preference for different habitats in northeastern Bristol Bay. Further study of potential variation in invertebrate community structure that may correlate with the observed interspecific spatial segregation in habitat use is needed to determine preferred forage and describe habitat requirements for each species. Such information is necessary to assess the potential impact that future anthropogenic or environmental changes may have on habitat quality of northeastern Bristol Bay and demography of Pacific sea duck populations that use this area.

Alaska

Vectors, hosts, and control measures for Zika virus in the Americas

We examine Zika virus (ZIKV) from an ecological perspective and with a focus on the Americas. We assess (1) the role of wildlife in ZIKV disease ecology, (2) how mosquito behavior and biology influence disease dynamics, and (3) how nontarget species and ecosystems may be impacted by vector control programs. Our review suggests that free-ranging, non-human primates may be involved in ZIKV transmission in the Old World; however, other wildlife species likely play a limited role in maintaining or transmitting ZIKV. In the Americas, a zoonotic cycle has not yet been definitively established. Understanding behaviors and habitat tolerances of Aedes aegypti and Aedes albopictus , two ZIKV competent vectors in the Americas, will allow more accurate modeling of disease spread and facilitate targeted and effective control efforts. Vector control efforts may have direct and indirect impacts to wildlife, particularly invertebrate feeding species; however, strategies could be implemented to limit detrimental ecological effects.

EcoHealth

Linking the agricultural landscape of the Midwest to stream health with structural equation modeling

Multiple physical and chemical stressors can simultaneously affect the biological condition of streams. To better understand the complex interactions of land-use practices, water quality, and ecological integrity of streams, the U.S. Geological Survey National Water Quality Assessment Project is conducting regional-scale assessments of stream condition across the United States. In the summer of 2013, weekly water samples were collected from 100 streams in the Midwestern United States. Employing watershed theory, we used structural equation modeling (SEM) to represent a general hypothesis for how 16 variables (previously identified to be important to stream condition) might be inter-related. Again, using SEM, we evaluated the ability of this “stressor network” to explain variations in multimetrics of algal, invertebrate, and fish community health, trimming away any environmental variables not contributing to an explanation of the ecological responses. Seven environmental variables—agricultural and urban land use, sand content of soils, basin area, percent riparian area as forest, channel erosion, and relative bed stability—were found to be important for all three-community metrics. The algal and invertebrate models included water-chemistry variables not included in the fish model. Results suggest that ecological integrity of Midwest streams are affected by both agricultural and urban land uses and by the natural geologic setting, as indicated by the sand content of soils. Chemicals related to crops (pesticides and nutrients) and residential uses (pyrethroids) were found to be more strongly related to ecological integrity than were natural factors (riparian forest, watershed soil character).

Environmental Science & Technology

Rivers and streams: Ecosystem dynamics and integrating paradigms

Full understanding of running waters requires an ecosystem perspective, which encompasses the physical and chemical setting in interaction with dependent biological communities. Several conceptual models or paradigms of river and stream ecosystems that capture critical components of lotic ecosystems have been developed, including the ‘river continuum concept’, to describe fluxes of matter and energy within the stream or river channel together with exchanges between the channel and its terrestrial setting. A complete ecosystem perspective includes consideration of hierarchical spatial scales in a temporal context. Flow of energy in lotic ecosystems is driven by two alternative energy sources: sunlight regulating in-stream photosynthesis and plant litter derived from the stream-side riparian corridor or floodplain. Energy transfers within the ecosystem pass through micro- and macroproducers (algae and vascular hydrophytes) and micro- and macroconsumers (microorganisms, invertebrates, and vertebrates). Material fluxes encompass the cycling of key nutrients, such as nitrogen and phosphorus, and the transport, storage, and metabolism of dissolved (DOM) and particulate (POM) organic matter (OM). Growth of lotic periphyton (algae and associated microbes, microzoans, and detritus) and coarse (CPOM) and fine (FPOM) particulate organic matter constitute the food resources of nonpredaceous running-water invertebrates (e.g., shredders that consume CPOM and collectors that feed on FPOM and associated microbes of both).

Book chapter

Characteristics of water, sediment, and benthic communities of the Wolf River, Menominee Indian Reservation, Wisconsin, water years 1986-98

Analyses and interpretation of water quality, sediment, and biological data from water years 1986 through 1998 indicated that land use and other human activities have had only minimal effects on water quality in the Wolf River upstream from and within the Menominee Indian Reservation in northeastern Wisconsin. Relatively high concentrations of calcium and magnesium (natural hardness), iron, manganese, and aluminum were measured in Wolf River water samples during water years 1986-98 from the three sampled sites and attributed to presence of highly mineralized geologic materials in the basin. Average calcium and magnesium concentrations varied from 22-26 milligrams per liter (mg/L) and 11-13 mg/L, respectively. Average iron concentrations ranged from 290-380 micrograms per liter ( μ g/L); average manganese concentrations ranged from 53-56 mg/L. Average aluminum concentrations ranged from 63-67 μ g/L. Mercury was present in water samples but concentrations were not at levels of concern. Levels of Kjeldahl nitrogen, ammonia, nitrite plus nitrate, total phosphorus, and orthophosphorus in water samples were often low or below detection limits (0.01- 0.10 mg/L). Trace amounts of atrazine (maximum concentration of 0.031 μ g/L), deethylatrazine (maximum 0.032 μ g/L), and alachlor (maximum of 0.002 μ g/L) were detected. Low concentrations of most trace elements were found in streambed sediment. Tissues of fish and aquatic invertebrates collected once each year from 1995 through 1998 at the Langlade and Keshena sites, near the northern and southern boundaries of the Reservation, respectively, were low in concentrations of most trace elements. Arsenic and silver in fish livers from both sites were less than or equal to 2 μ g/g arsenic and less than 1 μ g/g silver for dry weight analysis, and concentrations of antimony, beryllium, cadmium, cobalt, lead, nickel, and uranium were all below detection limits (less than 1 μ g/g dry weight). Concentrations of most other trace elements in fish were low, with the exceptions of chromium, copper, mercury, and selenium; however, these concentrations are not at levels of concern. Concentrations of all trace elements analyzed in whole caddisfly larvae also were low compared to those reported in the literature. During 1998, a total of 48 species of macroinvertebrates were identified at each of two sampled sites, with similar numbers of genera represented at both: 41 at Keshena and 44 at Langlade. The percentage EPT (Ephemeroptera, Plecoptera, and Trichoptera) was 52 at Keshena and 77 at Langlade; these relatively large percentages suggest very good to excellent water quality at these sites. A total of 52 algal taxa were identified at the Wolf River near Langlade. Diatoms made up 96 percent of the algal biomass. A total of 58 algal taxa were identified at Keshena, including 48 diatom taxa (83 percent). Although diatoms accounted for just 22 percent of the algal relative abundance, in cells per square centimeter, diatoms contributed 91 percent of the total algal biomass. The overall biological integrity of the Keshena and Langlade sites, based on diversity, siltation, and pollution indexes for diatoms is excellent.

Wisconsin

Contaminant Hazard Reviews (compilation)

This compact disc (CD) contains the 35 reports in the Contaminant Hazard Reviews (CHR) that were published originally between 1985 and 1999 in the U.S. Department of the Interior Biological Report series. The CD was produced because printed supplies of these reviews--a total of 105,000--became exhausted and demand remained high. Each review was prepared at the request of environmental specialists of the U.S. Fish and Wildlife Service and each contained specific information on the following: mirex, cadmium, carbofuran, toxaphene, selenium, chromium, polychlorinated biphenyls, dioxins, diazinon, mercury, polycyclic aromatic hydrocarbons, arsenic, chlorpyrifos, lead, tin, index issue, pentachlorophenol, atrazine, molybdenum, boron, chlordane, paraquat, cyanide, fenvalerate, diflubenzuron, zinc, famphur, acrolein, radiation, sodium monofluoroacetate, planar PCBs, silver, copper, nickel, and a cumulative index to chemicals and species. Each report reviewed and synthesized the technical literature on a single contaminant and its effects on terrestrial plants and invertebrates, aquatic plants and animals, avian and mammalian wildlife, and other natural resources. The subtopics include contaminant sources and uses; physical, chemical, and metabolic properties; concentrations in field collections of abiotic materials and living organisms; deficiency effects, where appropriate; lethal and sublethal effects, including effects on survival, growth, reproduction, metabolism, mutagenicity, teratogenicity, and carcinogenicity; proposed criteria for the protection of human health and sensitive natural resources; and recommendations for additional research.

Contaminant Hazard Reviews

Impacts of agricultural land use on biological integrity: A causal analysis

Agricultural land use has often been linked to nutrient enrichment, habitat degradation, hydrologic alteration, and loss of biotic integrity in streams. The U.S. Geological Survey's National Water Quality Assessment Program sampled 226 stream sites located in eight agriculture‐dominated study units across the United States to investigate the geographic variability and causes of agricultural impacts on stream biotic integrity. In this analysis we used structural equation modeling (SEM) to develop a national and set of regional causal models linking agricultural land use to measured instream conditions. We then examined the direct, indirect, and total effects of agriculture on biotic integrity as it acted through multiple water quality and habitat pathways. In our nation‐wide model, cropland affected benthic communities by both altering structural habitats and by imposing water quality‐related stresses. Region‐specific modeling demonstrated that geographic context altered the relative importance of causal pathways through which agricultural activities affected stream biotic integrity. Cropland had strong negative total effects on the invertebrate community in the national, Midwest, and Western models, but a very weak effect in the Eastern Coastal Plain model. In the Western Arid and Eastern Coastal Plain study regions, cropland impacts were transmitted primarily through dissolved water quality contaminants, but in the Midwestern region, they were transmitted primarily through particulate components of water quality. Habitat effects were important in the Western Arid model, but negligible in the Midwest and Eastern Coastal Plain models. The relative effects of riparian forested wetlands also varied regionally, having positive effects on biotic integrity in the Eastern Coastal Plain and Western Arid region models, but no statistically significant effect in the Midwest. These differences in response to cropland and riparian cover suggest that best management practices and planning for the mitigation of agricultural land use impacts on stream ecosystems should be regionally focused.

Ecological Applications

Benthos and plankton community data for selected rivers and harbors along Wisconsin's Lake Michigan shoreline, 2012

Four river systems on the Wisconsin shoreline of Lake Michigan are designated Areas of Concern (AOCs) because of severe environmental degradation: the Lower Menominee River, Lower Green Bay and Fox River, Sheboygan River, and Milwaukee Estuary. Each AOC has one or more Beneficial Use Impairments (BUIs) that form the basis of the AOC designation and that must be remediated or otherwise addressed before the AOC designation can be removed. All four of these AOCs have BUIs for benthos (bottom-dwelling or benthic invertebrates), and all but the Menominee River have a BUI for plankton (free-floating algae and invertebrates, or phytoplankton and zooplankton, respectively). The U.S. Geological Survey collected samples in 2012 at these four AOCs and at six non-AOCs to support the evaluation of the status of aquatic communities in the benthos and plankton at the AOCs. Samples were collected during three periods representing spring, summer, and fall. Benthos samples were collected using a dredge grab sampler and artificial substrates; plankton samples were collected using a tow net for zooplankton and a vertical water sampler for phytoplankton. Benthos and plankton were identified to the lowest possible taxonomic category and counted; samples for documenting water temperature, pH, and specific conductance, as well as sediment particle size and organic carbon were also collected during biological sampling.

Wisconsin

The story of the Penobscot River Ecology Mural: A 10-step process for scientists to create public art

Rivers are home to a wide variety of biota, including fishes, amphibians, reptiles, birds, freshwater mussels, aquatic insects, and microscopic organisms that fill unique niches to support broader ecosystem functions. While the general public may be aware of recreationally relevant biological life (e.g., fishes and insects to model flyfishing flies from), they may not be aware of more obscure yet important taxa. For example, freshwater mussels (Unionidae) improve water quality and clarity of rivers through filtration ( Vaughn, 2018), which is vital to riverine ecosystems. Despite this importance, lack of public knowledge about freshwater mussels is the second biggest challenge to their management ( Bouska et al., 2018). How do we best get the public to see value in underappreciated taxa to support their conservation? Public art installations can spread awareness about science effectively because they distill information for broader audiences, are eye-catching, and reach people through an emotional, aesthetic, and personal lens ( Thompson et al., 2023). While invertebrates are generally viewed by the public with fear and aversion ( Kellert, 1993) this can be ameliorated through interaction and learning, particularly for children ( Miller et al., 2025). Through cross-disciplinary collaboration between artists, scientists and the local community, we created the “Penobscot River Ecology Mural,” a scientifically informed artwork featuring underappreciated taxa like mussels and insects. In this article, we will elucidate the story of how we successfully made this piece through 10 steps, which provide a blueprint for future endeavors.

Maine

Distribution and effects of acidic deposition on wildlife and ecosystems

Acidic deposition occurs over most of the United States and the deposition patterns and theoretical vulnerabilities of aquatic ecosystems to chemical changes can be delineated, but few data exist on concomitant biological effects. Hypothetical direct effects are limited primarily to toxicity of various heavy metals mobilized at reduced pH. Results of studies in Scandinavia suggest that aluminum interferes with avian reproduction near acidified lakes. Some amphibian populations located on acid-vulnerable substrates may be adversely affected by reduced pH in the vernal pools used for egg laying and larval growth. Indirect effects on populations are difficult to detect because few historical data exist for wildlife populations and trophic relationships in vulnerable areas. Current research in the U.S.A. focuses on measuring habitat characteristics, food availability, and avian use of vulnerable wetland habitats. Results of Scandinavian studies suggest that some species of waterfowl may prefer acidified, I fish-free habitats because invertebrates essential for meeting nutritional requirements are more easily obtained in the absence of competition from fish. However, avian species dependent on fish would be absent from these habitats. Alteration of either the vegetative structure or primary productivity of wetlands might indirectly affect avian populations by causing decreased invertebrate productivity and consequent food limitations for birds.

Book chapter

Breakpoint analysis and assessment of selected stressor variables on benthic macroinvertebrate and fish communities in Indiana streams: Implications for developing nutrient criteria

Water chemistry, periphyton and seston chlorophyll a (CHL a ), and biological community data were collected from 321 sites from 2001 through 2005 to (1) determine statistically and ecologically significant relations among the stressor (total nitrogen, total phosphorus, periphyton and seston CHL a , and turbidity) variables and response (biological community) variables; and, (2) determine the breakpoint of biological community attributes and metrics in response to changes in stressor variables. Because of the typically weak relations among the stressor and response variables, methods were developed to reduce the effects of non-nutrient biological stressors that could mask the effect of nutrients. Stressor variable concentrations ranged from 0.30 to 11.0 milligrams per liter (mg/L) for total nitrogen, 0.025 to 1.33 mg/L for total phosphorus, 2.9 to 768 milligrams per square meter (mg/m 2 ) for periphyton CHL a , and 0.37 to 42 micrograms per liter (µg/L) for seston CHL a . Turbidity, another stressor variable, ranged from 0.8 to 65.4 Nephelometric turbidity units (NTUs). When the nutrient and CHL a data were compared to Dodds’ trophic classifications, 75.0 percent of the values for total nitrogen, 46.6 percent of the values for total phosphorus, 35.8 percent of the values for periphyton CHL a , and 3.5 percent of the values for seston CHL a , were eutrophic. The invertebrate communities were dominated by families considered highly nutrient tolerant, Chironimidae, (41.7 percent relative abundance), Hydropsychidae, (17.3 percent relative abundance), and Baetidae, (10.2 percent relative abundance). Fish communities were dominated by algivores and nutrient-tolerant species, specifically central stonerollers (13.3 percent relative abundance), creek chubs (9.9 percent relative abundance), and bluntnose minnows (9.3 percent relative abundance). Although not the dominant taxa, white sucker, spotted sucker, green sunfish, and bluegill species were correlated (p ‹0.05) with the stressor variables. The median breakpoints ranged from 2.4 to 3.3 mg/L for total nitrogen, from 0.042 to 0.129 mg/L for total phosphorus, from 54 to 68 mg/m 2 for periphyton CHL a , from 4.5 to 7.5 µg/L for seston CHL a , and from 14.1 to 16.1 NTU for turbidity. The breakpoints determined in this study, in addition to Dodds’ trophic classifications, were used as multiple lines of evidence to show changes in fish and invertebrate community and attributes based on annual exposure to nutrients.

Indiana

Examining historical mercury sources in the Saint Louis River estuary: How legacy contamination influences biological mercury levels in Great Lakes coastal regions

Industrial chemical contamination within coastal regions of the Great Lakes can pose serious risks to wetland habitat and offshore fisheries, often resulting in fish consumption advisories that directly affect human and wildlife health. Mercury (Hg) is a contaminant of concern in many of these highly urbanized and industrialized coastal regions, one of which is the Saint Louis River estuary (SLRE), the second largest tributary to Lake Superior. The SLRE has legacy Hg contamination that drives high Hg concentrations within sediments, but it is unclear whether legacy-derived Hg actively cycles within the food web. To understand the relative contributions of legacy versus contemporary Hg sources in coastal zones, Hg, carbon, and nitrogen stable isotope ratios were measured in sediments and food webs of SLRE and the Bad River, an estuarine reference site. Hg stable isotope values revealed that legacy contamination of Hg was widespread and heterogeneously distributed in sediments of SLRE, even in areas lacking industrial Hg sources. Similar isotope values were found in benthic invertebrates, riparian spiders, and prey fish from SLRE, confirming legacy Hg reaches the SLRE food web. Direct comparison of prey fish from SLRE and the Bad River confirmed that Hg isotope differences between the sites were not attributable to fractionation associated with rapid Hg bioaccumulation at estuarine mouths, but due to the presence of industrial Hg within SLRE. The Hg stable isotope values of game fish in both estuaries were dependent on fish migration and diet within the estuaries and extending into Lake Superior. These results indicate that Hg from legacy contamination is actively cycling within the SLRE food web and, through migration, this Hg also extends into Lake Superior via game fish. Understanding sources and the movement of Hg within the estuarine food web better informs restoration strategies for other impaired Great Lakes coastal zones.

Minnesota, Wisconsin

Diatom enumeration method influences biological assessments of southeastern USA streams

Current fixed-count enumeration methods for benthic diatoms are likely inadequate for most research and monitoring objectives. These methods underestimate taxa richness and may fail to detect losses of species caused by human impacts. Consequently, the full potential of diatoms is not realized in current assessments of biological integrity or species diversity. In this study, we hypothesize that alternative enumeration methods differ in their ability to quantify species composition. Furthermore, we hypothesize that an alternative to the traditional fixed-count method will improve both performance of observed/expected (O/E) indices derived from River Inver- tebrate Prediction and Classification System models and the discrimination of reference-quality and human-modified sites by other standard metrics used in biological assessments. To test these hypotheses, we assessed 1) how well 3 counting methods characterized species richness in a subset of 15 samples of stream benthic diatoms and 2) how counting method affected the performance of O/E indices and metrics by comparing the traditional fixed- count method against the best-performing alternative method. These latter comparisons were based on samples collected from 68 reference-quality streams and 20 streams located along an urban disturbance gradient. We dem- onstrate that traditional fixed counts failed to detect >1⁄2 of species present in most of the 68 reference-quality sites. Instead, timed-presence data produced the O/E index with the best performance and a level of precision similar to published invertebrate O/E indices. Furthermore, the O/E index based on the timed-presence data allowed us to determine which species are most often lost with urbanization. We found that traditional fixed-count and alter- native timed-presence data produce metrics that are nearly equally able to discriminate between reference and dis- turbed sites. This study demonstrates that alternative counting methods improve species detection and require up to ∼30% less effort.

Arkansas, Alabama, Georgia, Louisiana, Maryland, M

Cruise Report; RV Moana Wave cruise M1-01-GM; the bathymetry and acoustic backscatter of the mid shelf to upper slope off Panama City, Florida, northeastern Gulf of Mexico; September 3, through October 12, 2001, Panama City, FL to Panama City, FL

A zone of deep-water reefs is thought to extend from the mid and outer shelf south of Mississippi and Alabama to at least the northwestern Florida shelf off Panama City, Florida (Figure 1, 67kb). The reefs off Mississippi and Alabama are found in water depths of 60 to 120 m (Ludwick and Walton, 1957; Gardner et al., in press) and were the focus of a multibeam echosounder (MBES) mapping survey by the U.S. Geological Survey (USGS) in 2000 (Gardner et al., 2000; in press). If this deep-water-reef trend does exist along the northwestern Florida shelf, then it is critical to determine the accurate geomorphology and type of the reefs that occur because of their importance as benthic habitats for fisheries. Precisely georeferenced high-resolution mapping of bathymetry is a fundamental first step in the study of areas suspected to be critical habitats. Morphology is thought to be critical to defining the distribution of dominant demersal plankton/planktivores communities. Fish faunas of shallow hermatypic reefs have been well studied, but those of deep ahermatypic reefs have been relatively ignored. The ecology of deep-water ahermatypic reefs is fundamentally different from hermatypic reefs because autochthonous intracellular symbiotic zooxanthellae (the carbon source for hermatypic corals) do not form the base of the trophic web in ahermatypic reefs. Instead, exogenous plankton, transported to the reef by currents, serves as the primary carbon source. Thus, one of the principle uses of the morphology data will be to identify whether any reefs found are hermatypic or ahermatypic in origin. Community structure and trophodynamics of demersal fishes of the outer continental of the northeastern Gulf of Mexico presently are the focus of a major USGS reseach project. A goal of the project is to answer questions concerning the relative roles played by morphology and surficial geology in controling biological differentiation. Deep-water reefs are important because they are fish havens, key spawning sites, and are critical early larval and juvenile habitats for economically important sport/food fishes. It is known that deep-water reefs function as a key source for re-population (via seasonal and ontogenetic migration) of heavily impacted inshore reefs. The deep-water reefs south of Mississippi and Alabama support a lush fauna of ahermatypic hard corals, soft corals, black corals, sessile crinoids and sponges, that together form a living habitat for a well-developed fish fauna. The fish fauna comprises typical Caribbean reef fishes and Carolinian shelf fishes, plus epipelagic fishes, and a few deep-sea fishes. The base of the megafaunal invertebrate food web is plankton, borne by essentially continuous semi-laminar currents generated by eddies, spawned off the Loop Current, that periodically travel across the shelf edge. A few, sidescan-sonar surveys have been made of areas locally identified as Destin Pinnacles, Steamboat Lumps Marine Reserve (Koenig et al., 2000; Scanlon, et al., 2000; 2001), Twin Ridges (Briere, et al., 2000; Scanlon, et al., 2000), and Madison-Swanson Marine Reserve (Koenig et al., 2000; Scanlon, et al., 2000; 2001). However, no quantitative and little qualitative information about the geomorphology and surficial geology can be gained from these data. Existing bathymetry along the northwestern Florida shelf suggests the existence of areas of possible isolated deep-water reefs. NOAA bathymetric maps NOS NH16-9 and NG16-12 show geomorphic expressions that hint of the presence of reefs in isolated areas rather than in a continuous zone. There has been no systematic, high-resolution bathymetry collected in this area, prior to this cruise. After the successful mapping of the deep-water reefs on the Mississippi and Alabama shelf (Gardner et al., 2000; in press), a partnership composed of the USGS, Minerals Management Service, and NOAA was formed to continue the deep-reef mapping to the northwest Florida mid shelf and upper slope. This cruise is the first fruit of that partnership.

Florida