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Fish misidentification and potential implications to monitoring within the San Francisco Estuary, California

Fish monitoring programs often rely on the collection, species identification, and counting of individual fish over time to inform natural resource management decisions. Thus, the utility of the data used to inform these decisions can be negatively affected by species misidentification. Fish species misidentification bias can be minimized by confirming identification using genetic techniques, training observers, or adjusting monitoring data using estimates of incomplete detection and false-positive misidentification. Despite the existence of well-established fish identification training and quality control programs, there is considerable uncertainty about fish species false-positive misidentification rates and the effectiveness of fish identification training programs within the San Francisco Estuary. We evaluated the misidentification of fish species among Delta Juvenile Fish Monitoring Program observers by conducting five fish identification exams under controlled conditions at the Lodi Fish and Wildlife Office in Lodi, California, between 2012 and 2014. To assess the variability in false-positive misidentification, we fitted data to species and observer characteristics using hierarchical logistic regression. We found that fish species misidentification was fairly common, averaging 17% among 155 test specimens and 32 observers. False-positive misidentification varied considerably among species and was negatively related to fish size, the abundance of the species within monitoring samples, and observer experience. In addition, observers who were not formally trained or used as full-time observers were, on average, 6.0 times more likely to falsely identify a species. However, false-positive misidentification rates among observers and specimens still varied considerably after controlling for observer experience and training, and species and size, respectively. Our results could be used to improve fish identification training and testing, increase the accuracy of fish occupancy or abundance estimation, and justify the allocation of resources to continually use and formally train full-time observers within long-term monitoring programs operating in the system.

California

Life history, genetics, range expansion and new frontiers of the lionfish (Pterois volitans, Perciformes: Pteroidae) in Latin America

Pterois volitans (lionfish) is a midsize predatory fish commonly found in waters of the western Pacific and Indian Ocean. The species was first documented in Dania Beach, Florida (northwestern Caribbean) in 1985. Since that time the species has expanded its range rapidly to the Northwestern Atlantic Ocean, Gulf of Mexico, and Caribbean Sea. Since its introduction P. volitans has changed community structure and biodiversity of Caribbean reef communities and other coastal tropical ecosystems. Continuous introductions (accidental or intentional), limited natural predators, naïve-range prey behavior, high predation rates on competitors, continuous reproduction, and an extended period of larval dispersal have been the keys for successful invasion and rapid range extension of P. volitans . This invasion has become so severe that it has been recognized as one of the world’s top conservation issues. Here, we review the life history, behavior, and historical and contemporary genetic patterns that facilitate expansion and the colonization process. A greater understanding of lionfish biology, ecology, and the changes related to its present condition as a super-invader could improve current and future management strategies and new detection and response methodologies. We also examine new invasion frontiers that this species has the potential to colonize such as the eastern Pacific. This information will provide managers, the scientific community, and the civil society better tools for eradication, control and management of future invasions of this and other invasive species.

Caribbean Sea, Latin America

Hydrothermal vents and methane seeps: Rethinking the sphere of influence

Although initially viewed as oases within a barren deep ocean, hydrothermal vent and methane seep communities are now recognized to interact with surrounding ecosystems on the sea floor and in the water column, and to affect global geochemical cycles. The importance of understanding these interactions is growing as the potential rises for disturbance from oil and gas extraction, seabed mining and bottom trawling. Here we synthesize current knowledge of the nature, extent and time and space scales of vent and seep interactions with background systems. We document an expanded footprint beyond the site of local venting or seepage with respect to elemental cycling and energy flux, habitat use, trophic interactions, and connectivity. Heat and energy are released, global biogeochemical and elemental cycles are modified, and particulates are transported widely in plumes. Hard and biotic substrates produced at vents and seeps are used by “benthic background” fauna for attachment substrata, shelter, and access to food via grazing or through position in the current, while particulates and fluid fluxes modify planktonic microbial communities. Chemosynthetic production provides nutrition to a host of benthic and planktonic heterotrophic background species through multiple horizontal and vertical transfer pathways assisted by flow, gamete release, animal movements, and succession, but these pathways remain poorly known. Shared species, genera and families indicate that ecological and evolutionary connectivity exists among vents, seeps, organic falls and background communities in the deep sea; the genetic linkages with inactive vents and seeps and background assemblages however, are practically unstudied. The waning of venting or seepage activity generates major transitions in space and time that create links to surrounding ecosystems, often with identifiable ecotones or successional stages. The nature of all these interactions is dependent on water depth, as well as regional oceanography and biodiversity. Many ecosystem services are associated with the interactions and transitions between chemosynthetic and background ecosystems, for example carbon cycling and sequestration, fisheries production, and a host of non-market and cultural services. The quantification of the sphere of influence of vents and seeps could be beneficial to better management of deep-sea environments in the face of growing industrialization.

Frontiers in Marine Science

Implementation of genetic conservation practices in a muskellunge propagation and stocking program

Conservation of genetic resources is a challenging issue for agencies managing popular sport fishes. To address the ongoing potential for genetic risks, we developed a comprehensive set of recommendations to conserve genetic diversity of muskellunge ( Esox masquinongy ) in Wisconsin, and evaluated the extent to which the recommendations can be implemented. Although some details are specific to Wisconsin's muskellunge propagation program, many of the practical issues affecting implementation are applicable to other species and production systems. We developed guidelines to restrict future broodstock collection operations to lakes with natural reproduction and to develop a set of brood lakes to use on a rotational basis within regional stock boundaries, but implementation will require considering lakes with variable stocking histories. Maintaining an effective population size sufficient to minimize the risk of losing alleles requires limiting broodstock collection to large lakes. Recommendations to better approximate the temporal distribution of spawning in hatchery operations and randomize selection of brood fish are feasible. Guidelines to modify rearing and distribution procedures face some logistic constraints. An evaluation of genetic diversity of hatchery-produced fish during 2008 demonstrated variable success representing genetic variation of the source population. Continued evaluation of hatchery operations will optimize operational efficiency while moving toward genetic conservation goals.

Fisheries

A population on the rise: The origin of deepwater sculpin in Lake Ontario

Deepwater sculpin, Myoxocephalus thompsonii , were thought to have been extirpated from Lake Ontario. However, in recent years, abundance has increased and recruitment has been documented. There are two hypotheses concerning the origin of the current Lake Ontario deepwater sculpin population. First, individuals from the upper Great Lakes may have recolonized Lake Ontario. Alternatively, the Lake Ontario population may have not been extirpated, and the remnant population has recovered naturally. To test these hypotheses, eight microsatellite loci were used to analyze samples from the current Lake Ontario population, museum specimens from the historic Lake Ontario population, and current upper Great Lakes populations. The genetic data suggest that historically throughout the Great Lakes, deepwater sculpin exhibited low levels of spatial genetic structure. Approximate Bayesian Computation analyses support the hypothesis that the current Lake Ontario population is more closely related to populations in the upper Great Lakes than to the historic Lake Ontario samples, indicating that the current Lake Ontario population likely resulted from recolonization from the Upper Great Lakes. The current Lake Ontario population has reduced allelic diversity relative to upper Great Lakes populations, indicating a possible founder effect. This study demonstrates the role life history variation can play in recolonization success. The pelagic larval phase of the deepwater sculpin allowed recolonization of Lake Ontario via passive larval drift.

Lake Ontario

Mangroves and people: Impacts and interactions

Mangroves have long been associated with human populations, as coastal communities rely on the various ecosystem services that mangroves provide. However, human degradation and destruction of mangrove forests is common, despite and because of our reliance on them as valuable ecosystems. Mangrove research and management must elucidate and reconcile these conflicts to maintain mangrove forests and the services that humans depend on. To better understand the complex dynamics, interactions and relationships that exist between mangroves and coastal communities, the 5th Mangrove Macrobenthos and Management (MMM5) conference was held in Singapore in July 2019, with the theme “ Mangroves and People: Impacts and Interactions”. This theme was chosen because Southeast Asia is the epicentre of ongoing mangrove loss, and a large number of coastal communities in the region rely on the ecosystem services that mangroves provide. The 32 papers published in this Special Issue represent the breadth of mangrove research presented at MMM5, including topics in faunal biology, ecosystem ecology, genetics, physical geography, biogeochemistry, remote sensing and the social sciences. The articles are characterized under the following topics: 1) mangrove ecosystem functioning; 2) range expansion of mangroves; 3) ecosystem services of mangroves; 4) anthropogenic and natural threats to mangroves; and 5) the management and social-ecology of mangroves. This Special Issue highlights the current state of the art of mangrove research and application, and describes a number of emerging new ideas and research opportunities that will continue to advance mangrove ecosystem science into the future.

Estuarine, Coastal and Shelf Science

Genetic population structure of Shoal Bass within their native range

Endemic to the Apalachicola River basin of the southeastern USA, the Shoal Bass Micropterus cataractae is a fluvial‐specialist sport fish that is imperiled because of anthropogenic habitat alteration. To counter population declines, restorative stocking efforts are becoming an increasingly relevant management strategy. However, population genetic structure within the species is currently unknown, but it could influence management decisions, such as brood source location. Leveraging a collaborative effort to collect and genotype specimens with 16 microsatellite loci, our objective was to characterize hierarchical population structure and genetic differentiation of the Shoal Bass across its native range, including an examination of structuring mechanisms, such as relatedness and inbreeding levels. Specimens identified as Shoal Bass were collected from 13 distinct sites ( N ranged from 17 to 209 per location) and were then taxonomically screened to remove nonnative congeners and hybrids (pure Shoal Bass N ranged from 13 to 183 per location). Our results revealed appreciable population structure, with five distinct Shoal Bass populations identifiable at the uppermost hierarchical level that generally corresponded with natural geographic features and anthropogenic barriers. Substructure was recovered within several of these populations, wherein differences appeared related to spatial isolation and local population dynamics. An analysis of molecular variance revealed that 3.6% of the variation in our data set was accounted for among three larger river drainages, but substructure within each river drainage also explained an additional 8.9% of genetic variation, demonstrating that management at a scale lower than the river drainage level would likely best conserve genetic diversity. Results provide a population genetic framework that can inform future management decisions, such as brood source location, so that genetic diversity within and among populations is conserved and overall adaptability of the species is maintained.

Apalachicola–Chattahoochee–Flint River Basin

Evolutionary and ecological connectivity in westslope cutthroat trout (Oncorhynchus clarkii lewisi) and mountain whitefish (Prosopium williamsoni) in relation to the potential influences of Boundary Dam, Washington, Idaho, and parts of British Columbia

In this report, we consider evolutionary and ecological connectivity for westslope cutthroat trout ( Oncorhynchus clarkii lewisi ) and mountain whitefish ( Prosopium williamsoni ) within the Pend Oreille River in northeastern Washington State, northern Idaho, and adjacent portions of southeastern British Columbia, Canada. Specifically, we focused on the rationale for active translocation of individuals of these species upstream from Boundary Dam both in the context of natural patterns of pre-dam evolutionary connectivity as well as preserving contemporary ecological and evolutionary characteristics of local extant populations. Boundary Dam impounds the Pend Oreille River (called the Pend d’Oreille River in Canada) with the resulting reservoir inundating two historical barriers to upstream movement of fish (Metaline Falls and Z Canyon). Historically, it was thought these barriers impeded the upstream movement of westslope cutthroat trout and mountain whitefish, as well as Pacific salmon ( Oncorhynchus spp.), steelhead trout ( O. mykiss ), and other resident species such as bull trout ( Salvelinus confluentus ). To address connectivity, we consider historical and contemporary processes and features. This review includes an assessment of postglacial processes within the Pend Oreille River and systems upstream that include Priest Lake, Lake Pend Oreille, the Clark Fork River, features of Boundary Reservoir and its tributaries, and areas downstream in the Pend Oreille River such as the Salmo River. Based on this information, we then give a more detailed review of existing genetic and ecological data to summarize what is known about connectivity for westslope cutthroat trout and mountain whitefish. Our assessment of the collective evidence leads us to conclude that moving fish upstream over Boundary Dam is not warranted if the management objective is to maintain natural patterns of evolutionary and ecological connectivity or to conserve unique ecological and evolutionary characteristics of extant local populations of these species in the system. These findings parallel that of a previous analysis of bull trout. Although we were able to arrive at well-supported conclusions in relation to Boundary Dam, we suggest that more work on connectivity further upstream in the Pend Oreille River would help to better understand the role of historical processes and dams further up in the system.

British Columbia, Idaho, Washington

Using fecal DNA and closed-capture models to estimate feral horse population size

Accurate population estimates provide the foundation for managing feral horses ( Equus caballus ferus ) across the western United States. Certain feral horse populations are protected by the Wild and Free-Roaming Horses and Burros Act of 1971 and managed by the Bureau of Land Management (BLM) or the United States Forest Service on designated herd management areas (HMAs) or wild horse territories, respectively. Horses are managed to achieve an appropriate management level (AML), which represents the number of horses determined by BLM to contribute to a thriving natural ecological balance and avoid deterioration of the range. To achieve AML for each HMA, BLM resource managers need accurate and precise population estimates. We tested the use of non-invasive fecal samples in a genetic capture-recapture framework to estimate population size in a closed horse population at the Little Book Cliffs HMA, Colorado, USA, with a known size of 153 individuals. We collected 1,957 samples over 3 independent sampling periods in 2014 and amplified them at 8 microsatellite loci. We applied mark-recapture models to determine population size using 954 samples that amplified at all 8 loci. We subsampled and reanalyzed our dataset to simulate different data collection protocols and evaluated effects on accuracy and precision of estimates using N-mixture modeling, full likelihood closed-capture modeling, and capwire single-occasion modeling that used data from all 3 sampling periods. Our model results were accurate and precise for analyses that used data from all 3 occasions; however, capwire single-occasion modeling was not accurate when we analyzed each sampling period separately. For all subsampling analysis scenarios, reducing sample size decreased precision, whether by reducing number of field staff, field days, or geographic areas surveyed on each period. Reducing spatial coverage of the survey area did not result in accurate population estimates and only marginally lowered the number of samples that would need to be collected to maintain accuracy. Because laboratory analysis contributes the greatest expense for this method ($80 U.S./sample), reducing fecal sample size is advantageous. Our results demonstrate that non-invasive sampling combined with good survey design and careful genetic and capture-recapture analyses can provide an alternative method to estimate the number of feral horses in a closed population. This method may be especially appropriate in situations where aerial inventories are not practical or accurate because of low sighting conditions. But the higher costs associated with laboratory sample analyses may reduce the method's feasibility compared to helicopter surveys.

Colorado

Bacterial Cycling of Methyl Halides

This chapter focuses on the monohalogenated methanes methyl chloride (MeCl) and methyl bromide (MeBr), their natural and anthropogenic sources, and their degradation by microorganisms, specifically by aerobic bacteria that can use MeBr and MeCl as sole source of carbon and energy. The biogeochemical cycle of methyl halides and the microbiology, biochemistry, genetics, and biotechnological potential of methyl halide-degrading microorganisms are discussed in the chapter. Methyl halides are the dominant halocarbons in the atmosphere. They play an important role in regulating stratospheric ozone concentrations and global warming as well, two factors governing planetary habitability. The monohalomethanes—methyl chloride (MeCl), methyl bromide (MeBr), and methyl iodide (MeI)—are trace gases in the atmosphere with average tropospheric-mixing ratios of 600, 10, and 2 parts per trillion (ppt), respectively. However, methyl halides are radiatively active and hence contribute to global warming by absorbing radiation in the infrared region. This is evident in their elevated global warming potential (GWP), a value calculated to quantify each compound's warming effect (on a mass basis) relative to the same mass of CO 2 . Compounds with high GWP including those like methyl halides with low concentrations may have considerable impact on atmospheric warming when compared with other “greenhouse” gases with low GWP.

Advances in Applied Microbiology

Loss of dendritic connectivity in southern California's urban riverscape facilitates decline of an endemic freshwater fish

Life history adaptations and spatial configuration of metapopulation networks allow certain species to persist in extreme fluctuating environments, yet long-term stability within these systems relies on the maintenance of linkage habitat. Degradation of such linkages in urban riverscapes can disrupt this dynamic in aquatic species, leading to increased extinction debt in local populations experiencing environment-related demographic flux. We used microsatellites and mtDNA to examine the effects of collapsed network structure in the endemic Santa Ana sucker Catostomus santaanae of southern California, a threatened species affected by natural flood-drought cycles, ‘boom-and-bust’ demography, hybridization, and presumed artificial transplantation. Our results show a predominance of drift-mediated processes in shaping population structure, and that reverse mechanisms for counterbalancing the genetic effects of these phenomena have dissipated with the collapse of dendritic connectivity. We use approximate Bayesian models to support two cases of artificial transplantation, and provide evidence that one of the invaded systems better represents the historic processes that maintained genetic variation within watersheds than any remaining drainages where C. santaanae is considered native. We further show that a stable dry gap in the northern range is preventing genetic dilution of pure C. santaanae persisting upstream of a hybrid assemblage involving a non-native sucker, and that local accumulation of genetic variation in the same drainage is influenced by position within the network. This work has important implications for declining species that have historically relied on dendritic metapopulation networks to maintain source-sink dynamics in phasic environments, but no longer possess this capacity in urban-converted landscapes.

California

Discovery of a novel hepatovirus (Phopivirus of seals) related to human hepatitis A virus

Describing the viral diversity of wildlife can provide interesting and useful insights into the natural history of established human pathogens. In this study, we describe a previously unknown picornavirus in harbor seals (tentatively named phopivirus) that is related to human hepatitis A virus (HAV). We show that phopivirus shares several genetic and phenotypic characteristics with HAV, including phylogenetic relatedness across the genome, a specific and seemingly quiescent tropism for hepatocytes, structural conservation in a key functional region of the type III internal ribosomal entry site (IRES), and a codon usage bias consistent with that of HAV.

mBio

Development of genomic markers for monitoring and research on plethodontid salamanders

Despite the importance of plethodontid salamanders and their vulnerability to ongoing environmental change, they are inherently difficult to monitor due to their cryptic nature. Recent advances in genomics have created new opportunities for monitoring of populations and their responses to environmental perturbations. In this study, we developed a new target capture-based genomic panel for the purposes of genetic monitoring in plethodontid salamanders. We demonstrate its utility in several distantly related species and present an example application in two representative species with co-occurring distributions but different ecological attributes and expected patterns of population structure: Plethodon jordani and Desmognathus wrighti . Although the number of successfully assembled loci declined with phylogenetic distance from the original reference species ( Desmognathus spp), we obtained high-quality data from thousands of loci from species in all four genera tested ( Desmognathus , Plethodon , Eurycea , and Gyrinophilus ), which span the deepest split in Plethodontidae. Landscape genetic analyses detected weak but statistically significant geographic structure in P. jordani , and much stronger geographic structure in D. wrighti , as expected based on the lower population density and likely lower dispersal ability of D. wrighti . Our target capture panel is broadly applicable across salamanders in Plethodontidae and has the potential to provide data for a wide range of phylogenetic, biogeographic, and population genetics research questions.

North Carolina, Tennessee

Spatial capture–recapture for categorically marked populations with an application to genetic capture–recapture

Recently introduced unmarked spatial capture–recapture (SCR), spatial mark–resight (SMR), and 2‐flank spatial partial identity models (SPIMs) extend the domain of SCR to populations or observation systems that do not always allow for individual identity to be determined with certainty. For example, some species do not have natural marks that can reliably produce individual identities from photographs, and some methods of observation produce partial identity samples as is the case with remote cameras that sometimes produce single‐flank photographs. Unmarked SCR, SMR, and SPIM share the feature that they probabilistically resolve the uncertainty in individual identity using the spatial location where samples were collected. Spatial location is informative of individual identity in spatially structured populations because a sample is more likely to have been produced by an individual living near the trap where it was recorded than an individual living further away from the trap. Further, the level of information about individual identity that a spatial location contains is related to two key ecological concepts, population density and home range size, which we quantify using a proposed Identity Diversity Index (IDI). We show that latent and partial identity SCR models produce imprecise and biased density estimates in many high IDI scenarios when data are sparse. We then extend the unmarked SCR model to incorporate categorical, partially identifying covariates, which reduce the level of uncertainty in individual identity, increasing the reliability and precision of density estimates, and allowing reliable density estimation in scenarios with higher IDI values and with more sparse data. We illustrate the performance of this “categorical SPIM” via simulations and by applying it to a black bear data set using microsatellite loci as categorical covariates, where we reproduce the full data set estimates with only slightly less precision using fewer loci than necessary for confident individual identification. We then discuss how the categorical SPIM can be applied to other wildlife sampling scenarios such as remote camera surveys, where natural or researcher‐applied partial marks can be observed in photographs. Finally, we discuss how the categorical SPIM can be added to SMR, 2‐flank SPIM, or other latent identity SCR models.

Ecosphere

Estimating the power of a standardized monitoring program for sportfish in Georgia, USA

Objective Biological monitoring is a major component of management decisions and operating budgets of many natural resource management agencies. Given the scientific and financial commitments to monitoring, it is critical to estimate the ability to detect trends through time (i.e., power). Methods The Georgia Department of Natural Resources has monitored reservoir sport fish populations since the 1980s. We estimated the power to detect simulated long-term (≥10 years) changes in relative abundance (CPUE) for Largemouth Bass (some of which are a potential genetic admixture of the recently described species Micropterus nigricans [now known as Largemouth Bass] and M. salmoides [now known as Florida Bass)] and Black Crappie Pomoxis nigromaculatus sampled with electrofishing and gill nets, respectively, across multiple reservoirs ( n = 21). Reservoir-specific simulations were parameterized using 13 years (∼2010–2022) of monitoring data. Power was calculated as the proportion of simulations ( n = 1,000) resulting in significant ( P ≤ 0.1) temporal trends across a 10-year period. We considered power ≥0.8 (i.e., 80% of simulations with significant trends) as the threshold for sufficient power across reservoirs. For both species, we estimated power under three scenarios: (1) declining CPUE, (2) increasing CPUE, and (3) reduction to biennial sampling effort with a 50% decline in CPUE. Results Most reservoirs had sufficient power to detect either a 50% decline or a 100% increase in CPUE of Largemouth Bass across a 10-year period. Switching from annual to biennial sampling for Largemouth Bass reduced the number of reservoirs with sufficient power by half. Power was generally lower for Black Crappie until larger declines (75%) or increases (400%) were imposed. Conclusions We found that Largemouth Bass monitoring was generally near or beyond our reference threshold, but post hoc correlation analyses suggested that the power of Black Crappie data could be increased with more within-reservoir station replication. Overall, using data simulation to estimate power proved a valuable tool in assessing the potential ability of common monitoring approaches to detect change.

Georgia

SNP discovery in candidate adaptive genes using exon capture in a free-ranging alpine ungulate

Identification of genes underlying genomic signatures of natural selection is key to understanding adaptation to local conditions. We used targeted resequencing to identify SNP markers in 5321 candidate adaptive genes associated with known immunological, metabolic and growth functions in ovids and other ungulates. We selectively targeted 8161 exons in protein-coding and nearby 5′ and 3′ untranslated regions of chosen candidate genes. Targeted sequences were taken from bighorn sheep ( Ovis canadensis ) exon capture data and directly from the domestic sheep genome ( Ovis aries v. 3; oviAri3). The bighorn sheep sequences used in the Dall's sheep ( Ovis dalli dalli ) exon capture aligned to 2350 genes on the oviAri3 genome with an average of 2 exons each. We developed a microfluidic qPCR-based SNP chip to genotype 476 Dall's sheep from locations across their range and test for patterns of selection. Using multiple corroborating approaches ( lositan and bayescan ), we detected 28 SNP loci potentially under selection. We additionally identified candidate loci significantly associated with latitude, longitude, precipitation and temperature, suggesting local environmental adaptation. The three methods demonstrated consistent support for natural selection on nine genes with immune and disease-regulating functions (e.g. Ovar-DRA, APC, BATF2, MAGEB18), cell regulation signalling pathways (e.g. KRIT1, PI3K, ORRC3), and respiratory health (CYSLTR1). Characterizing adaptive allele distributions from novel genetic techniques will facilitate investigation of the influence of environmental variation on local adaptation of a northern alpine ungulate throughout its range. This research demonstrated the utility of exon capture for gene-targeted SNP discovery and subsequent SNP chip genotyping using low-quality samples in a nonmodel species.

Molecular Ecology Resources

Movement patterns of Antillean manatees in Chetumal Bay (Mexico) and coastal Belize: A challenge for regional conservation

Information from 15 satellite-tracked Antillean manatees (Trichechus manatus manatus) was analyzed in order to assess individual movements, home ranges, and high-use areas for conservation decisions. Manatees were captured in Chetumal Bay, Mexico, and tagged with Argos-monitored satellite transmitters. Location of the manatees and physical characteristics were assessed to describe habitat properties. Most manatees traveled to freshwater sources. The Maximum Area Size (MAS) for each manatee was determined using the observation-area method. Additional kernel densities of 95% home range and 50% Center of Activity (COA) were also calculated, with manatees having 1–3 COAs. Manatees exhibited two different movement patterns: remaining in Chetumal Bay, and long-distance (up to 240 km in 89 d). The residence time in Chetumal Bay was higher for females (89.6% of time) than for males (72.0%), but the daily travel rate (0.4–0.5 km/d) was similar for both sexes. Most of the COAs fell within Natural Protected Areas (NPA). However, manatees also travel for long distances into unprotected areas, where they face uncontrolled boat traffic, fishing activities, and habitat loss. Conservation of movement corridors may promote long-distance movements and facilitate genetic exchange.

Chetumal Bay

Pb-Sr-Nd isotopes in surficial materials at the Pebble Porphyry Cu-Au-Mo Deposit, Southwestern Alaska: can the mineralizing fingerprint be detected through cover?

The Cretaceous Pebble porphyry Cu-Au-Mo deposit is covered by tundra and glacigenic sediments. Pb-Sr-Nd measurements were done on sediments and soils to establish baseline conditions prior to the onset of mining operations and contribute to the development of exploration methods for concealed base metal deposits of this type. Pebble rocks have a moderate range for 206 Pb/ 204 Pb = 18.574 to 18.874, 207 Pb/ 204 Pb = 15.484 to 15.526, and 208, Pb/ 204 Pb = 38.053 to 38.266. Mineralized granodiorite shows a modest spread in 87 Sr/ 86 Sr (0.704354–0.707621) and 143 Nd/ 144 Nd (0.512639–0.512750). Age-corrected (89 Ma) values for the granodiorite yield relatively unradiogenic Pb (e.g., 207 Pb/ 204 Pb <15.52), low values of 87 Sr/ 86 Sr, and positive values of ɛNd (1.00–4.52) that attest to a major contribution of mantle-derived source rocks. Pond sediments and soils have similar Pb isotope signatures and 87 Sr/ 86 Sr and 143 Nd/ 144 Nd values that resemble the mineralized granodiorites. Glacial events have obscured the recognition of isotope signatures of mineralized rocks in the sediments and soils. Baseline radiogenic isotope compositions, prior to the onset of mining operations, reflect natural erosion, transport and deposition of heterogeneous till sheets that included debris from barren rocks, mineralized granodiorite and sulfides from the Pebble deposit, and other country rocks that pre- and postdate the mineralization events. Isotopic variations suggest that natural weathering of the deposit is generally reflected in these surficial materials. The isotope data provide geochemical constraints to glimpse through the extensive cover and together with other geochemical observations provide a vector to concealed mineralized rocks genetically linked with the Pebble deposit.

Alaska