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Toxicity of silicon carbide nanowires to sediment-dwelling invertebrates in water or sediment exposures

Silicon carbide nanowires (SiCNW) are insoluble in water. When released into an aquatic environment, SiCNW would likely accumulate in sediment. The objective of this study was to assess the toxicity of SiCNW to four freshwater sediment-dwelling organisms: amphipods ( Hyalella azteca ), midges ( Chironomus dilutus ), oligochaetes ( Lumbriculus variegatus ), and mussels ( Lampsilis siliquoidea ). Amphipods were exposed to either sonicated or nonsonicated SiCNW in water (1.0 g/L) for 48 h. Midges, mussels, and oligochaetes were exposed only to sonicated SiCNW in water for 96 h. In addition, amphipods were exposed to sonicated SiCNW in whole sediment for 10 d (44% SiCNW on dry wt basis). Mean 48-h survival of amphipods exposed to nonsonicated SiCNW in water was not significantly different from the control, whereas mean survival of amphipods exposed to sonicated SiCNW in two 48-h exposures (0 or 15% survival) was significantly different from the control (90 or 98% survival). In contrast, no effect of sonicated SiCNW was observed on survival of midges, mussels, or oligochaetes. Survival of amphipods was not significantly reduced in 10-d exposures to sonicated SiCNW either mixed in the sediment or layered on the sediment surface. However, significant reduction in amphipod biomass was observed with the SiCNW either mixed in sediment or layered on the sediment surface, and the reduction was more pronounced for SiCNW layered on the sediment. These results indicated that, under the experimental conditions, nonsonicated SiCNW in water were not acutely toxic to amphipods, sonicated SiCNW in water were acutely toxic to the amphipods, but not to other organisms tested, and sonicated SiCNW in sediment affected the growth but not the survival of amphipods.

Environmental Toxicology and Chemistry

Conceptual models governing leaching behavior and their long-term predictive capability

Six models that may be used to describe the interaction of radioactive waste solids with aqueous solutions are as follows: Simple linear mass transfer; Simple parabolic mass transfer; Parabolic mass transfer with the formation of a diffusion-limiting surface layer at an arbitrary time; Initial parabolic mass transfer followed by linear mass transfer at an arbitrary time; Parabolic (or linear) mass transfer and concomitant surface sorption; and Parabolic (or linear) mass transfer and concomitant chemical precipitation. Some of these models lead to either illogical or unrealistic predictions when published data are extrapolated to long times. These predictions result because most data result from short-term experimentation. Probably for longer times, processes will occur that have not been observed in the shorter experiments. This hypothesis has been verified by mass-transfer data from laboratory experiments using natural volcanic glass to predict the composition of groundwater. That such rate-limiting mechanisms do occur is reassuring, although now it is not possible to deduce a single mass-transfer limiting mechanism that could control the solution concentration of all components of all waste forms being investigated. Probably the most reasonable mechanisms are surface sorption and chemical precipitation of the species of interest. Another is limiting of mass transfer by chemical precipitation on the waste form surface of a substance not containing the species of interest, that is, presence of a diffusion-limiting layer. The presence of sorption and chemical precipitation as factors limiting mass transfer has been verified in natural groundwater systems, whereas the diffusion-limiting mechanism has not been verified yet.

Nuclear and Chemical Waste Management

Biological nitrogen fixation across major biomes in Latin America: Patterns and global change effects

Biological nitrogen fixation (BNF) supports terrestrial primary productivity and plays key roles in mediating human-induced changes in global nitrogen (N) and carbon cycling. However, there are still critical uncertainties in our understanding of the amount of BNF occurring across terrestrial ecosystems, and of how terrestrial BNF will respond to global change. We synthesized BNF data from Latin America, a region reported to sustain some of the highest BNF rates on Earth, but that is underrepresented in previous data syntheses. We used meta-analysis and modeling approaches to estimate BNF rates across Latin America's major biomes and to evaluate the potential effects of increased N deposition and land-use change on these rates. Unmanaged tropical and subtropical moist forests sustained observed and predicted total BNF rates of 10 ± 1 and 14 ± 1 kg N ha −1 y −1 , respectively, supporting the hypothesis that these forests sustain lower BNF rates than previously thought. Free-living BNF accounted for two-thirds of the total BNF in these forests. Despite an average 30% reduction of free-living BNF in response to experimental N-addition, our results suggest free-living BNF rate responses to current and projected N deposition across tropical and subtropical moist forests are small. In contrast, the conversion of unmanaged ecosystems to crop and pasture lands increased BNF rates across all terrestrial biomes, mostly in savannas, grasslands, and dry forests, increasing BNF rates 2-fold. The information obtained here provides a more comprehensive understanding of BNF patterns for Latin America.

Science of the Total Environment

Behavioral responses of Silver Carp to underwater acoustic deterrent sounds

Objective Invasive carps continue to spread across the Mississippi River basin, posing significant ecological risk. Identifying technologies to slow their dispersal is critical. The use of sound has been proposed as a method to modify the behavior of Silver Carp Hypophthalmichthys molitrix , offering a nonstructural deterrent strategy. Methods Silver Carp implanted with acoustic transmitters were released into earthen ponds equipped with telemetry arrays. The fish were exposed to a 30-min playback of three underwater sounds (chirp saw, chirp square, and 100-hp boat motor). Movement trajectories were analyzed using a two-state hidden Markov model to estimate the effects of environmental and experimental variables on fish behavior. Results The results of the hidden Markov model supported two behavioral states. State 1 was characterized by longer step lengths (distance between positions) and greater directional persistence in turning angle (change in direction between two intervals), indicative of heighted activity. State 2 was defined by shorter step lengths and less directional persistence, suggesting reduced activity. Silver Carp that were exposed to the chirp square sound had an increased likelihood of entering state 1, whereas the 100-hp boat motor sound promoted transitions to state 2. Conclusions Underwater sounds distinctly influenced the movement of Silver Carp in earthen ponds. The chirp square sound elicited heightened activity levels, demonstrating potential for use in acoustic deterrent applications. However, the response of Silver Carp to these sounds may be influenced by the size of the study environment or the absence of natural drivers of fish behavior, such as food or reproduction. This study contributes to the development of nonstructural, species-specific deterrent systems by identifying sounds that influence the behavior of invasive carps. The application of sound-based methods may play a critical role in integrated pest management strategies for invasive carps, potentially limiting their spread while minimizing effects on native species.

Transactions of the American Fisheries Society

Permeameter data verify new turbulence process for MODFLOW

A sample of Key Largo Limestone from southern Florida exhibited turbulent flow behavior along three orthogonal axes as reported in recently published permeameter experiments. The limestone sample was a cube measuring 0.2 m on edge. The published nonlinear relation between hydraulic gradient and discharge was simulated using the turbulent flow approximation applied in the Conduit Flow Process (CFP) for MODFLOW-2005 mode 2, CFPM2. The good agreement between the experimental data and the simulated results verifies the utility of the approach used to simulate the effects of turbulent flow on head distributions and flux in the CFPM2 module of MODFLOW-2005.

Ground Water

Predicting performance for ecological restoration: A case study using Spartina altemiflora

The success of population-based ecological restoration relies on the growth and reproductive performance of selected donor materials, whether consisting of whole plants or seed. Accurately predicting performance requires an understanding of a variety of underlying processes, particularly gene flow and selection, which can be measured, at least in part, using surrogates such as neutral marker genetic distances and simple latitudinal effects. Here we apply a structural equation modeling approach to understanding and predicting performance in a widespread salt marsh grass, Spartina alterniflora, commonly used for ecological restoration throughout its native range in North America. We collected source materials from throughout this range, consisting of eight clones each from 23 populations, for transplantation to a common garden site in coastal Louisiana and monitored their performance. We modeled performance as a latent process described by multiple indicator variables (e.g., clone diameter, stem number) and estimated direct and indirect influences of geographic and genetic distances on performance. Genetic distances were determined by comparison of neutral molecular markers with those from a local population at the common garden site. Geographic distance metrics included dispersal distance (the minimum distance over water between donor and experimental sites) and latitude. Model results indicate direct effects of genetic distance and latitude on performance variation among the donor sites. Standardized effect strengths indicate that performance was roughly twice as sensitive to variation in genetic distance as to latitudinal variation. Dispersal distance had an indirect influence on performance through effects on genetic distance, indicating a typical pattern of genetic isolation by distance. Latitude also had an indirect effect on genetic distance through its linear relationship with dispersal distance. Three performance indicators had significant loadings on performance alone (mean clone diameter, mean number of stems, mean number of inflorescences), while the performance indicators mean stem height and mean stem width were also influenced by latitude. We suggest that dispersal distance and latitude should provide an adequate means of predicting performance in future S. alterniflora restorations and propose a maximum sampling distance of 300 km (holding latitude constant) to avoid the sampling of inappropriate ecotypes. ?? 2010 by the Ecological Society of America.

Ecological Applications

Water movement in the unsaturated zone at a low-level radioactive-waste burial site near Barnwell, South Carolina

Four unsaturated zone monitoring sites and a meteorologic station were installed at the low level radioactive waste burial site near Barnwell, South Carolina, to investigate the geohydrologic and climatologic factors affecting water movement in the unsaturated zone. The study site is located in the Atlantic Coastal Plain. The unsaturated zone consists of a few centimeters to > 1 m of surface sand, underlain by up to 15 m of clayey sand. Two monitoring sites were installed in experimental trenches and two were installed in radioactive waste trenches. Two different trench designs were evaluated at the monitoring sites. A meteorologic station was used to measure precipitation and to calculate actual evapotranspiration using the Bowen ratio method. Soil-moisture tensiometers, soil-moisture conductance probes, and temperature sensors were used to monitor soil-water movement in and adjacent to the trenches. Tracer tests using sodium chloride were conducted at each monitoring site. Data collection at the monitoring sites began in January 1982 and continued until early May 1984. Tensiometer data show that the unsaturated materials had their highest percent saturations in the winter and spring. Saturations in the backfill sand varied from 20 to 100%. They varied from about 75 to 100% in the adjacent undisturbed and overlying compacted clayey sand. Additionally, because tensiometer data indicate negligible water storage changes in the unsaturated zone, it is estimated that approximately 43 cm of recharge reached the water table. During 1984, the rise and fall of ponded water in an experimental trench was continuously monitored with a digital recorder. A cross-sectional finite element model of variably saturated flow was used to test the conceptual model of water movement in the unsaturated zone and to illustrate the effect of trench design on water movement into the experimental trenches. Monitoring and model results show that precipitation on trenches infiltrated the trench cap and moved vertically into the trench backfill material. The trench construction practice of placing a compacted clayey-sand barrier around the trench greatly inhibits soil water from entering the trench. (Author 's abstract)

South Carolina

[Book review] The Eastern Screech Owl: Life History, Ecology and Behavior in the Suburbs and Countryside, by Frederick Gehlback

Review of: Eastern Screech Owl: Life History, Ecology, and Behavior in the Suburbs and Countryside. Frederick R. Gehlbach. Issue 16; Issue 2008 of W. L. Moody Jr. Natural History Series. Texas A&M University Press; 1st edition (November 1994). ISBN: 0890966095. For ornithologists and ecologists alike, Fred Gehlbach's book promises to hold both interest and information value as a comprehensive study of the eastern screech owl (Otus asio hasbroucki). Gehlbach was intrigued with screech owls as a boy and encouraged as an undergraduate by William Hamilton, who underscored that in-depth studies of familiar backyard species can be as fascinating as those in exotic sites. Correspondence with another owl-aficionado, the late H. N. Southern, inspired the author's long-term study of screech owls in a woodland landscape in central Texas and led him to provide nest boxes to enhance his access and sample size. This book is based on observations over a 25-year period-beginning in 1967, with intensive study during an 11-year period (1976-1987) in Texas south of Waco, where Gehlbach teaches at Baylor University. The study represents observations on 659 screech owls, covering several generations of birds and entire lives of many individuals. Gehlbach compares screech owl nesting behavior in a rural versus suburban setting and includes chapters on food supplies and predation tactics; egg-laying, incubation, and parental behavior; vocalizations; and population structure and flux. He discusses why screech owls are widespread across the eastern half of North America and why they succeed among people in suburban environments, where they adapt as easily to mailboxes and porch columns as to natural tree cavities. The book mixes two approaches: on the one hand the dense style of a technical book in which the professional biologist can find information on many aspects of screech owl behavior, life history, and population, including tables, figures, summary statistics, results of statistical comparisons, and experimental tests of hypotheses. On the other hand, each chapter begins with and includes vivid visual images, personal experiences, and thoughtful reflections to draw in the general reader, bridging the interesting topic to the technical details and making the information accessible to a wider audience. The reader is impressed with Dr. Gehlbach's love of his subject, his devotion to long-term study, and his care in presenting the work to his fellow ornithologists, ecologists, and all those interested in wildlife and conservation. It will be a useful reference because of its wealth of information on all aspects of screech owl life history. It is well documented and, in addition to its 10 chapters, includes 10 appendices with information on types of food supplies, bird species that mob screech owls, a daily chronology of nestling development, and a selection of edited field notes to provide background for each chapter. The book is thoroughly referenced and indexed. The Eastern Screech Owl will be read with interest by those with an interest in long-term, in-depth studies and those who love charming stories about remarkable birds with strong personalities and interesting behavior.

Wildlife Review

Dual-domain mass-transfer parameters from electrical hysteresis: Theory and analytical approach applied to laboratory, synthetic streambed, and groundwater experiments

Models of dual‐domain mass transfer (DDMT) are used to explain anomalous aquifer transport behavior such as the slow release of contamination and solute tracer tailing. Traditional tracer experiments to characterize DDMT are performed at the flow path scale (meters), which inherently incorporates heterogeneous exchange processes; hence, estimated “effective” parameters are sensitive to experimental design (i.e., duration and injection velocity). Recently, electrical geophysical methods have been used to aid in the inference of DDMT parameters because, unlike traditional fluid sampling, electrical methods can directly sense less‐mobile solute dynamics and can target specific points along subsurface flow paths. Here we propose an analytical framework for graphical parameter inference based on a simple petrophysical model explaining the hysteretic relation between measurements of bulk and fluid conductivity arising in the presence of DDMT at the local scale. Analysis is graphical and involves visual inspection of hysteresis patterns to (1) determine the size of paired mobile and less‐mobile porosities and (2) identify the exchange rate coefficient through simple curve fitting. We demonstrate the approach using laboratory column experimental data, synthetic streambed experimental data, and field tracer‐test data. Results from the analytical approach compare favorably with results from calibration of numerical models and also independent measurements of mobile and less‐mobile porosity. We show that localized electrical hysteresis patterns resulting from diffusive exchange are independent of injection velocity, indicating that repeatable parameters can be extracted under varied experimental designs, and these parameters represent the true intrinsic properties of specific volumes of porous media of aquifers and hyporheic zones.

Water Resources Research

Experimental deformation of polycrystalline H2O ice at high pressure and low temperature: Preliminary results

Interest in the mechanical properties of water ice under the conditions in which it exists in the outer solar system has motivated the development and use of a new high‐pressure, low‐temperature triaxial deformation apparatus. Constant displacement rate tests on 70 samples of pure polycrystalline water ice have been performed at temperatures 77≤≤258 K, confining pressures 0.1≤≤350 MPa, and strain rates 3.5×10≤ ≤3.5×10 s. In most cases, the ice polymorph tested was ice . Both brittle and ductile behavior have been observed. Brittle behavior of ice, promoted by lower pressure, lower temperature, and higher strain rate, is analogous to that in rocks, with the important exception that brittle fracture strength becomes independent of confining pressure above 50 MPa pressure and the fracture angle is approximately 45° to the loading direction (i.e., the coefficient of internal friction is approximately zero). Ductile flow, the predominant behavior in our tests at ≥195 K, follows a law of form = σ exp (−*/) (σ is stress; is the gas constant; , * are material constants). Three sets of material constants are required to fit the data, with changes in sets (or mechanisms) occurring near 243 K and 195 K. The value of remains near 4 throughout the measured ductile field, but * drops from 91 to 61 to 31 /mole as temperature decreases. The maximum brittle strength measured was 171 MPa; the maximum ductile strength measured was 91 MPa. At confining pressures near the phase transition pressure of ice → ice II, the ductile strength is observed to drop dramatically. Some overlap with previous work occurs at higher temperatures and lower pressures. Agreement with present work is generally good, both quantitatively in the values of and *, and qualitatively in the mechanism of deformation. Although the ductile strengths measured here are somewhat higher than expected on the basis of extrapolations of previous work, the low value of * at <195 K indicates that the ice layer on icy bodies in the solar system is much weaker than has generally been predicted.

Journal of Geophysical Research B: Solid Earth

Tropical understory herbaceous community responds more strongly to hurricane disturbance than to experimental warming

The effects of climate change on tropical forests may have global consequences due to the forests’ high biodiversity and major role in the global carbon cycle. In this study, we document the effects of experimental warming on the abundance and composition of a tropical forest floor herbaceous plant community in the Luquillo Experimental Forest, Puerto Rico. This study was conducted within Tropical Responses to Altered Climate Experiment (TRACE) plots, which use infrared heaters under free‐air, open‐field conditions, to warm understory vegetation and soils + 4°C above nearby control plots. Hurricanes Irma and María damaged the heating infrastructure in the second year of warming, therefore, the study included one pretreatment year, one year of warming, and one year of hurricane response with no warming. We measured percent leaf cover of individual herbaceous species, fern population dynamics, and species richness and diversity within three warmed and three control plots. Results showed that one year of experimental warming did not significantly affect the cover of individual herbaceous species, fern population dynamics, species richness, or species diversity. In contrast, herbaceous cover increased from 20% to 70%, bare ground decreased from 70% to 6%, and species composition shifted pre to posthurricane. The negligible effects of warming may have been due to the short duration of the warming treatment or an understory that is somewhat resistant to higher temperatures. Our results suggest that climate extremes that are predicted to increase with climate change, such as hurricanes and droughts, may cause more abrupt changes in tropical forest understories than longer‐term sustained warming.

Puerto Rico

Nutritional requirements and feeding of selected coolwater fishes: A review

A review was made of published and unpublished studies on nutritional requirements , diets, and feeding of selected coolwater species of fishes : yellow perch (Perca flavescens), European perch (P. fluviatilis), walleye (Stizostedion vitreum), smallmouth bass (Micropterus dolomieui), northern pike (Esox lucius), muskellunge (E. masquinongy), and the northern pike x muskellunge hybrid. The published information on nutritional requirements was meager, including only preliminary studies of iodine and vitamin A. Most of the results of the feeding trials were unpublished and lacked experimental control so that the effect of diet was not always clearly demonstrated; however, the results were tabulated and summarized. Results indicated that considerable research is needed. The compositions of diets were given when available.

Progressive Fish-Culturist

Quantifying differences in the impact of variable chemistry on equilibrium uranium(VI) adsorption properties of aquifer sediments

Uranium adsorption-desorption on sediment samples collected from the Hanford 300-Area, Richland, WA varied extensively over a range of field-relevant chemical conditions, complicating assessment of possible differences in equilibrium adsorption properties. Adsorption equilibrium was achieved in 500-1000 h although dissolved uranium concentrations increased over thousands of hours owing to changes in aqueous chemical composition driven by sediment-water reactions. A nonelectrostatic surface complexation reaction, >SOH + UO 2 2+ + 2CO 3 2- = >SOUO 2 (CO 3 HCO 3 ) 2- , provided the best fit to experimental data for each sediment sample resulting in a range of conditional equilibrium constants (log K c ) from 21.49 to 21.76. Potential differences in uranium adsorption properties could be assessed in plots based on the generalized mass-action expressions yielding linear trends displaced vertically by differences in log K c values. Using this approach, log K c values for seven sediment samples were not significantly different. However, a significant difference in adsorption properties between one sediment sample and the fines (<0.063 mm) of another could be demonstrated despite the fines requiring a different reaction stoichiometry. Estimates of log K c uncertainty were improved by capturing all data points within experimental errors. The mass-action expression plots demonstrate that applying models outside the range of conditions used in model calibration greatly increases potential errors.

Washington

Multiple deleterious effects of experimentally aged sperm in a monogamous bird

Sperm aging is known to be detrimental to reproductive performance. However, this apparently general phenomenon has seldom been studied in an evolutionary context. The negative impact of sperm aging on parental fitness should constitute a strong selective pressure for adaptations to avoid its effects. We studied the impact of sperm aging on black-legged kittiwakes ( Rissa tridactyla ), a monogamous seabird. Kittiwakes comprise a model system because ( i ) of evidence that females eject their mates' sperm to prevent fertilization by sperm that would be old and degraded by the time of fertilization and result in reduced reproductive performance and ( ii ) the lack of extra-pair fertilization in this species makes cryptic female choice an unlikely explanation of postcopulatory sperm ejection by females. We experimentally manipulated the age of the sperm fertilizing kittiwake eggs by fitting males with anti-insemination rings for variable periods of time preceding egg-laying. We found evidence that sperm aging negatively affected four sequential stages of reproduction: fertilization potential, rate of embryonic development, embryonic mortality, and chick condition at hatching. These results may be produced by a continuum of a single process of sperm aging that differentially affects various aspects of development, depending on the degree of damage incurred to the spermatozoa. The marked impact of sperm age on female fitness may thus drive postcopulatory sperm ejection by females. These results provide experimental evidence of deleterious effects of sperm aging on a nondomestic vertebrate, underlining its taxonomic generality and its potential to select for a wide array of adaptations.

PNAS

Differential responses of coolwater fishes to a whole-lake reduction of a warmwater thermal guild

Climate change is transforming the ecology of lakes at a rapid pace, shifting some lakes toward warmwater-dominant habitats. As a result, warmwater fishes are increasingly becoming more prevalent in lakes where they already existed, altering the patterning and strength of species interactions. Understanding shifting species interactions (e.g., competition, predation), and the role of lake management in shaping these interactions, will be critical for lake stewardship in response to climate change. Here, we present results from an intensive 5-year experimental removal of ~285,000 warmwater fishes from a north-temperate lake. The goal of the experiment was to test whether warmwater fish reduction is effective for rewiring lake food webs to reverse undesirable conditions for coolwater species, leading to increased recruitment and abundance of coolwater fishes. Throughout the experiment, warmwater fishes were resilient to reductions, with biomass declines of 23% averaged across five species. Among coolwater fishes, the top predator walleye showed no biomass response, while yellow perch biomass increased by ~914%. Fish species biomass changes translated to food web shifts, including a yellow perch trophic position decline of 0.4, decreased zooplankton abundances, and increased zoobenthos abundances. Our results highlight differential species responses to a management action aimed at adapting to climate change. Despite similar thermal tolerances, two coolwater species responded differently to removal of warmwater fishes, highlighting the characteristics (e.g., life history strategies, adaptive capacity) that contribute to species resilience. Given the importance of biotic interactions, climate adaptation may need to go beyond a “one-size-fits-all” approach even when species have similar thermal tolerances.

Wisconsin

The accuracy of capture per unit effort in predicting density of a cryptic snake was more sensitive to reductions in spatial than temporal coverage

A critical component of monitoring wildlife populations is understanding changes in population size or abundance. However, for most populations a complete census is not possible; thus, trends or abundance need to be estimated through alternative means, such as indexes. An important aspect of using indexes, such as capture per unit effort (CPUE), is validating them as accurate or precise predictors of population trends or abundance. We completed such analyses using data collected from visual surveys and trapping for brown treesnakes ( Boiga irregularis ) within a 5-ha enclosure that was undergoing a continuous population decline. During a ~ 6-year period, we censused and marked the snake population to fully enumerated the population, with new individuals resulting from births and removals resulting only from mortality (natural or experimental). From trapping and visual surveys, we were also able to calculate CPUE as a function of trap nights or km surveyed and used regressions to forecast snake density (snakes/km) in the enclosure from CPUE. We also rarefied the true dataset to measure whether reductions in sampling intensity, either temporally or spatially, affected the accuracy or precision in predicting snake density from CPUE. We found that trap CPUE demonstrated no statistical relationship to density based on our study methods. CPUE during visual surveys did predict actual density, with sufficient spatial and temporal sampling intensity. CPUE from visual surveys was relatively robust against reductions in temporal sampling when spatial intensity remained high. However, reductions in the spatial area covered to less than 50% of the enclosure rapidly reduced the accuracy and precision in using CPUE to forecast density. Our results indicate that visual surveys are a relatively accurate measure of true density for brown treesnakes, given sufficient spatial sampling effort. The spatial area of coverage required for CPUE to accurately predict changes in abundance was, however, intense with > 50% of the spatial area required to be sampled on a given sampling night. Our results indicate that CPUE is only reliable as an index of abundance or population trends for cryptic snakes, if sampling effort covers most of the landscape over which populations are being estimated.

Guam

Chloroethene dechlorination in acidic groundwater: Implications for combining fenton's treatment with natural attenuation

A sulfuric acid leak in 1988 at a chloroethene-contaminated groundwater site at the Naval Air Station Pensacola has resulted in a long-term record of the behavior of chloroethene contaminants at low pH and a unique opportunity to assess the potential impact of source area treatment technologies, which involve acidification of the groundwater environment (e.g., Fenton's-based in situ chemical oxidation), on downgradient natural attenuation processes. The greater than 75 percent decrease in trichloroethene (TCE) concentrations and the shift in contaminant composition toward predominantly reduced daughter products (dichloroethene [DCE] and vinyl chloride [VC]) that were observed along a 30-m groundwater flow path characterized by highly acidic conditions (pH = 3.5 ± 0.4) demonstrated that chloroethene reductive dechlorination can continue to be efficient under persistent acidic conditions. The detection of Dehalococcoides -type bacteria within the sulfuric acid/chloroethene co-contaminant plume was consistent with biotic chloroethene reductive dechlorination. Microcosm studies conducted with 14 C-TCE and 14 C-VC confirmed biotic reductive dechlorination in sediment collected from within the sulfuric acid/chloroethene co-contaminant plume. Microcosms prepared with sediment from two other locations within the acid plume, however, demonstrated only a limited mineralization to 14 CO 2 and 14 CO, which was attributed to abiotic degradation because no significant differences were observed between experimental and autoclaved control treatments. These results indicated that biotic and abiotic mechanisms contributed to chloroethene attenuation in the acid plume at NAS Pensacola and that remediation techniques involving acidification of the groundwater environment (e.g., Fenton's-based source area treatment) do not necessarily preclude efficient chloroethene degradation.

Florida

Flat-plate techniques for measuring reflectance of macro-algae ( Ulva curvata )

We tested the consistency and accuracy of flat-plate spectral measurements (400&ndash;1000 nm) of the marine macrophyte Ulva curvata . With sequential addition of Ulva thallus layers, the reflectance progressively increased from 6% to 9% with six thalli in the visible (VIS) and from 5% to 19% with ten thalli in the near infrared (NIR). This progressive increase was simulated by a mathematical calculation based on an Ulva thallus diffuse reflectance weighted by a transmittance power series. Experimental and simulated reflectance differences that were particularly high in the NIR most likely resulted from residual water and layering structure unevenness in the experimental progression. High spectral overlap existed between fouled and non-fouled Ulva mats and the coexistent lagoon mud in the VIS, whereas in the NIR, spectral contrast was retained but substantially dampened by fouling.

International Journal of Remote Sensing